Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG014448 | XP_024634655.1 | 69.178 | 146 | 33 | 3 | 89 | 231 | 56 | 192 | 3.54e-54 | 183 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG014448 | sp|F4IN23|BZP34_ARATH | 41.053 | 95 | 56 | 0 | 125 | 219 | 186 | 280 | 7.47e-14 | 72.8 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG014448 | tr|A0A1S3E8J2|A0A1S3E8J2_CICAR | 61.321 | 106 | 41 | 0 | 123 | 228 | 148 | 253 | 5.87e-31 | 125 |
Gene ID | Type | Classification |
---|---|---|
MsaG014448 | TF | bZIP |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000705 | MsaG014448 | 0.816487 | 9.363138e-52 | 1.879605e-49 |
MsaG001092 | MsaG014448 | 0.804751 | 3.139149e-49 | 4.731684e-47 |
MsaG004445 | MsaG014448 | 0.805925 | 1.786429e-49 | 2.767366e-47 |
MsaG004455 | MsaG014448 | 0.817256 | 6.304222e-52 | 1.290897e-49 |
MsaG006284 | MsaG014448 | 0.839252 | 3.263542e-57 | 1.244459e-54 |
MsaG010113 | MsaG014448 | 0.806602 | 1.287860e-49 | 2.027362e-47 |
MsaG010807 | MsaG014448 | 0.820965 | 9.110145e-53 | 2.055551e-50 |
MsaG011213 | MsaG014448 | 0.802264 | 1.022836e-48 | 1.455717e-46 |
MsaG011678 | MsaG014448 | 0.813293 | 4.744560e-51 | 8.786445e-49 |
MsaG012026 | MsaG014448 | 0.812403 | 7.417008e-51 | 1.343488e-48 |
MsaG012396 | MsaG014448 | 0.803302 | 6.260872e-49 | 9.125523e-47 |
MsaG044869 | MsaG014448 | 0.826630 | 4.347919e-54 | 1.144259e-51 |
MsaG046278 | MsaG014448 | 0.825922 | 6.397286e-54 | 1.650908e-51 |
MsaG002644 | MsaG014448 | 0.803449 | 5.837744e-49 | 8.537866e-47 |
MsaG014448 | MsaG013395 | 0.801902 | 1.213556e-48 | 1.712961e-46 |
MsaG014448 | MsaG018949 | 0.809466 | 3.185170e-50 | 5.369931e-48 |
MsaG014448 | MsaG020111 | 0.812270 | 7.928402e-51 | 1.431299e-48 |
MsaG014448 | MsaG031416 | 0.814004 | 3.315037e-51 | 6.249751e-49 |
MsaG014448 | MsaG030749 | 0.806337 | 1.463836e-49 | 2.289965e-47 |
MsaG014448 | MsaG044124 | 0.805119 | 2.631042e-49 | 3.999874e-47 |
MsaG016372 | MsaG014448 | 0.800665 | 2.167635e-48 | 2.975205e-46 |
MsaG017558 | MsaG014448 | 0.800253 | 2.627768e-48 | 3.573166e-46 |
MsaG017561 | MsaG014448 | 0.837991 | 6.885355e-57 | 2.524550e-54 |
MsaG022579 | MsaG014448 | 0.803162 | 6.689634e-49 | 9.719206e-47 |
MsaG024877 | MsaG014448 | 0.804990 | 2.799578e-49 | 4.243312e-47 |
MsaG026280 | MsaG014448 | 0.807759 | 7.346480e-50 | 1.188630e-47 |
MsaG026826 | MsaG014448 | 0.816780 | 8.053517e-52 | 1.628946e-49 |
MsaG026901 | MsaG014448 | 0.831062 | 3.722930e-55 | 1.110695e-52 |
MsaG029621 | MsaG014448 | 0.816949 | 7.381304e-52 | 1.499531e-49 |
MsaG033531 | MsaG014448 | 0.818242 | 3.786030e-52 | 7.951676e-50 |
MsaG033589 | MsaG014448 | 0.845345 | 8.076339e-59 | 3.742949e-56 |
MsaG033651 | MsaG014448 | 0.810326 | 2.084503e-50 | 3.588211e-48 |
MsaG036580 | MsaG014448 | 0.810177 | 2.244174e-50 | 3.848875e-48 |
MsaG038522 | MsaG014448 | 0.805784 | 1.911149e-49 | 2.950861e-47 |
MsaG039823 | MsaG014448 | 0.829597 | 8.455800e-55 | 2.419174e-52 |
MsaG041854 | MsaG014448 | 0.807799 | 7.206072e-50 | 1.167031e-47 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG014448 | MtrunA17_Chr4g0040891 | 41.053 | 95 | 56 | 0 | 125 | 219 | 194 | 288 | 6.69e-15 | 72.8 |
MsaG014448 | MtrunA17_Chr2g0325131 | 40.000 | 95 | 57 | 0 | 125 | 219 | 191 | 285 | 5.59e-14 | 70.1 |
MsaG014448 | MtrunA17_Chr2g0316551 | 35.398 | 113 | 68 | 1 | 107 | 219 | 195 | 302 | 1.40e-13 | 68.9 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG014448 | AT2G42380.2 | 41.053 | 95 | 56 | 0 | 125 | 219 | 186 | 280 | 7.59e-15 | 72.8 |
MsaG014448 | AT5G07160.1 | 42.105 | 114 | 65 | 1 | 104 | 217 | 28 | 140 | 1.44e-14 | 69.3 |
MsaG014448 | AT3G58120.1 | 36.441 | 118 | 65 | 1 | 102 | 219 | 189 | 296 | 1.53e-14 | 72.0 |
MsaG014448 | AT2G42380.5 | 41.772 | 79 | 46 | 0 | 125 | 203 | 186 | 264 | 5.01e-12 | 64.3 |
MsaG014448 | AT3G58120.2 | 36.275 | 102 | 55 | 1 | 102 | 203 | 189 | 280 | 5.60e-12 | 64.3 |
Find 32 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
CAGCAATTTCAAGTCTTAAT+TGG | 0.217186 | 3:-73346224 | None:intergenic |
ACTCGATTATATTGCAGATT+TGG | 0.254957 | 3:+73345569 | MsaT014448.1:CDS |
CTGCTCGGGATTCACGGTTT+TGG | 0.270588 | 3:-73339283 | None:intergenic |
ATCTGCTTGGATCACAATTT+AGG | 0.298827 | 3:+73339314 | MsaT014448.1:CDS |
GAATAGGCTGAGGGAGCTTC+AGG | 0.311474 | 3:+73346388 | MsaT014448.1:CDS |
GCACTGCTCATTAGCTTAGT+TGG | 0.388270 | 3:-73339083 | None:intergenic |
TGCTTGTTCCTTCAACATCA+TGG | 0.393720 | 3:-73339151 | None:intergenic |
ATAAGAAAGCTGAGGTGAAT+AGG | 0.428995 | 3:+73346372 | MsaT014448.1:CDS |
TATTGCAGATTTGGAACAGA+TGG | 0.461511 | 3:+73345578 | MsaT014448.1:CDS |
TTTCATAGGCTTCAACTTGT+GGG | 0.474934 | 3:-73346170 | None:intergenic |
AAATTAACACCAGCAGCATC+AGG | 0.482919 | 3:-73339248 | None:intergenic |
CTTCAAACCCTCCTCTTCCA+TGG | 0.485844 | 3:+73339058 | MsaT014448.1:CDS |
AAGAAGAGCCATGATGTTGA+AGG | 0.491822 | 3:+73339143 | MsaT014448.1:CDS |
AGCAGGAAAATCTATCTGCT+TGG | 0.498692 | 3:+73339301 | MsaT014448.1:CDS |
TTTGCAACTCCTGATGCTGC+TGG | 0.512964 | 3:+73339239 | MsaT014448.1:CDS |
CTTAGTTGGAGCCATGGAAG+AGG | 0.515801 | 3:-73339069 | None:intergenic |
CAATTTCAAGTCTTAATTGG+TGG | 0.516892 | 3:-73346221 | None:intergenic |
AAGCTGAGGTGAATAGGCTG+AGG | 0.536145 | 3:+73346378 | MsaT014448.1:CDS |
CATTAGCTTAGTTGGAGCCA+TGG | 0.548196 | 3:-73339075 | None:intergenic |
CTAGTTCATCCTTTCAAGTG+TGG | 0.562525 | 3:+73339105 | MsaT014448.1:CDS |
AGGCTTCAACTTGTGGGCGA+AGG | 0.567374 | 3:-73346164 | None:intergenic |
AAGCAGCAAGATCAACATAG+TGG | 0.577317 | 3:+73339169 | MsaT014448.1:CDS |
AGTTGGAGCCATGGAAGAGG+AGG | 0.583574 | 3:-73339066 | None:intergenic |
GTTGGAGCCATGGAAGAGGA+GGG | 0.592854 | 3:-73339065 | None:intergenic |
CAAAACCGTGAATCCCGAGC+AGG | 0.606708 | 3:+73339284 | MsaT014448.1:CDS |
AAACAAAGGATACTTCAAGA+AGG | 0.610927 | 3:+73346255 | MsaT014448.1:CDS |
TTTCTGAAGCACAAAACAAG+AGG | 0.611292 | 3:+73346420 | MsaT014448.1:CDS |
AGAGGAGGGTTTGAAGCTCA+CGG | 0.618456 | 3:-73339051 | None:intergenic |
AGCTGAGGTGAATAGGCTGA+GGG | 0.623733 | 3:+73346379 | MsaT014448.1:CDS |
GATTGAGAATAAGAAAGCTG+AGG | 0.643843 | 3:+73346364 | MsaT014448.1:CDS |
AAGCCTATGAAAATCAGCAG+CGG | 0.717215 | 3:+73346181 | MsaT014448.1:CDS |
GGAGGGTTTGAAGCTCACGG+TGG | 0.736332 | 3:-73339048 | None:intergenic |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr3 | gene | 73338978 | 73346552 | 73338978 | ID=MsaG014448 |
Chr3 | mRNA | 73338978 | 73346552 | 73338978 | ID=MsaT014448.1;Parent=MsaG014448 |
Chr3 | exon | 73338978 | 73339369 | 73338978 | ID=MsaT014448.1.exon1;Parent=MsaT014448.1 |
Chr3 | CDS | 73338978 | 73339369 | 73338978 | ID=cds.MsaT014448.1;Parent=MsaT014448.1 |
Chr3 | exon | 73345524 | 73345614 | 73345524 | ID=MsaT014448.1.exon2;Parent=MsaT014448.1 |
Chr3 | CDS | 73345524 | 73345614 | 73345524 | ID=cds.MsaT014448.1;Parent=MsaT014448.1 |
Chr3 | exon | 73346150 | 73346276 | 73346150 | ID=MsaT014448.1.exon3;Parent=MsaT014448.1 |
Chr3 | CDS | 73346150 | 73346276 | 73346150 | ID=cds.MsaT014448.1;Parent=MsaT014448.1 |
Chr3 | exon | 73346360 | 73346441 | 73346360 | ID=MsaT014448.1.exon4;Parent=MsaT014448.1 |
Chr3 | CDS | 73346360 | 73346441 | 73346360 | ID=cds.MsaT014448.1;Parent=MsaT014448.1 |
Chr3 | exon | 73346540 | 73346552 | 73346540 | ID=MsaT014448.1.exon5;Parent=MsaT014448.1 |
Chr3 | CDS | 73346540 | 73346552 | 73346540 | ID=cds.MsaT014448.1;Parent=MsaT014448.1 |
Gene Sequence |
Protein sequence |