AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014448


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014448 MtrunA17_Chr4g0040891 41.053 95 56 0 125 219 194 288 6.69e-15 72.8
MsaG014448 MtrunA17_Chr2g0325131 40.000 95 57 0 125 219 191 285 5.59e-14 70.1
MsaG014448 MtrunA17_Chr2g0316551 35.398 113 68 1 107 219 195 302 1.40e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014448 AT2G42380.2 41.053 95 56 0 125 219 186 280 7.59e-15 72.8
MsaG014448 AT5G07160.1 42.105 114 65 1 104 217 28 140 1.44e-14 69.3
MsaG014448 AT3G58120.1 36.441 118 65 1 102 219 189 296 1.53e-14 72.0
MsaG014448 AT2G42380.5 41.772 79 46 0 125 203 186 264 5.01e-12 64.3
MsaG014448 AT3G58120.2 36.275 102 55 1 102 203 189 280 5.60e-12 64.3

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCAATTTCAAGTCTTAAT+TGG 0.217186 3:-73346224 None:intergenic
ACTCGATTATATTGCAGATT+TGG 0.254957 3:+73345569 MsaT014448.1:CDS
CTGCTCGGGATTCACGGTTT+TGG 0.270588 3:-73339283 None:intergenic
ATCTGCTTGGATCACAATTT+AGG 0.298827 3:+73339314 MsaT014448.1:CDS
GAATAGGCTGAGGGAGCTTC+AGG 0.311474 3:+73346388 MsaT014448.1:CDS
GCACTGCTCATTAGCTTAGT+TGG 0.388270 3:-73339083 None:intergenic
TGCTTGTTCCTTCAACATCA+TGG 0.393720 3:-73339151 None:intergenic
ATAAGAAAGCTGAGGTGAAT+AGG 0.428995 3:+73346372 MsaT014448.1:CDS
TATTGCAGATTTGGAACAGA+TGG 0.461511 3:+73345578 MsaT014448.1:CDS
TTTCATAGGCTTCAACTTGT+GGG 0.474934 3:-73346170 None:intergenic
AAATTAACACCAGCAGCATC+AGG 0.482919 3:-73339248 None:intergenic
CTTCAAACCCTCCTCTTCCA+TGG 0.485844 3:+73339058 MsaT014448.1:CDS
AAGAAGAGCCATGATGTTGA+AGG 0.491822 3:+73339143 MsaT014448.1:CDS
AGCAGGAAAATCTATCTGCT+TGG 0.498692 3:+73339301 MsaT014448.1:CDS
TTTGCAACTCCTGATGCTGC+TGG 0.512964 3:+73339239 MsaT014448.1:CDS
CTTAGTTGGAGCCATGGAAG+AGG 0.515801 3:-73339069 None:intergenic
CAATTTCAAGTCTTAATTGG+TGG 0.516892 3:-73346221 None:intergenic
AAGCTGAGGTGAATAGGCTG+AGG 0.536145 3:+73346378 MsaT014448.1:CDS
CATTAGCTTAGTTGGAGCCA+TGG 0.548196 3:-73339075 None:intergenic
CTAGTTCATCCTTTCAAGTG+TGG 0.562525 3:+73339105 MsaT014448.1:CDS
AGGCTTCAACTTGTGGGCGA+AGG 0.567374 3:-73346164 None:intergenic
AAGCAGCAAGATCAACATAG+TGG 0.577317 3:+73339169 MsaT014448.1:CDS
AGTTGGAGCCATGGAAGAGG+AGG 0.583574 3:-73339066 None:intergenic
GTTGGAGCCATGGAAGAGGA+GGG 0.592854 3:-73339065 None:intergenic
CAAAACCGTGAATCCCGAGC+AGG 0.606708 3:+73339284 MsaT014448.1:CDS
AAACAAAGGATACTTCAAGA+AGG 0.610927 3:+73346255 MsaT014448.1:CDS
TTTCTGAAGCACAAAACAAG+AGG 0.611292 3:+73346420 MsaT014448.1:CDS
AGAGGAGGGTTTGAAGCTCA+CGG 0.618456 3:-73339051 None:intergenic
AGCTGAGGTGAATAGGCTGA+GGG 0.623733 3:+73346379 MsaT014448.1:CDS
GATTGAGAATAAGAAAGCTG+AGG 0.643843 3:+73346364 MsaT014448.1:CDS
AAGCCTATGAAAATCAGCAG+CGG 0.717215 3:+73346181 MsaT014448.1:CDS
GGAGGGTTTGAAGCTCACGG+TGG 0.736332 3:-73339048 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 73338978 73346552 73338978 ID=MsaG014448
Chr3 mRNA 73338978 73346552 73338978 ID=MsaT014448.1;Parent=MsaG014448
Chr3 exon 73338978 73339369 73338978 ID=MsaT014448.1.exon1;Parent=MsaT014448.1
Chr3 CDS 73338978 73339369 73338978 ID=cds.MsaT014448.1;Parent=MsaT014448.1
Chr3 exon 73345524 73345614 73345524 ID=MsaT014448.1.exon2;Parent=MsaT014448.1
Chr3 CDS 73345524 73345614 73345524 ID=cds.MsaT014448.1;Parent=MsaT014448.1
Chr3 exon 73346150 73346276 73346150 ID=MsaT014448.1.exon3;Parent=MsaT014448.1
Chr3 CDS 73346150 73346276 73346150 ID=cds.MsaT014448.1;Parent=MsaT014448.1
Chr3 exon 73346360 73346441 73346360 ID=MsaT014448.1.exon4;Parent=MsaT014448.1
Chr3 CDS 73346360 73346441 73346360 ID=cds.MsaT014448.1;Parent=MsaT014448.1
Chr3 exon 73346540 73346552 73346540 ID=MsaT014448.1.exon5;Parent=MsaT014448.1
Chr3 CDS 73346540 73346552 73346540 ID=cds.MsaT014448.1;Parent=MsaT014448.1
Gene Sequence

>MsaT014448.1

ATGCGAGAAGAAAATGCAAAGAAAATCAATTTTCTGAGCTCAAAAAAGGATAAGTTATGTGCTGTGAAATCCACCGTGAGCTTCAAACCCTCCTCTTCCATGGCTCCAACTAAGCTAATGAGCAGTGCTAGTTCATCCTTTCAAGTGTGGAAAAATGCTTTACAAAAGAAGAGCCATGATGTTGAAGGAACAAGCAGCAAGATCAACATAGTGGAAAACCCTATTCTTGATCAAGCTAAAAAGAATATCATCAATCTTAGCTTTGCAACTCCTGATGCTGCTGGTGTTAATTTAATTAACACCAACCAAAACCGTGAATCCCGAGCAGGAAAATCTATCTGCTTGGATCACAATTTAGGCAAAACCGAAGAAGATCCAAAAATACTTAAGCGGATTGAAGCAAATCGAGCTGCTGCACAAAAATCGAGATTGAAAAAACTCGATTATATTGCAGATTTGGAACAGATGGAAAAATATTTGCAGGCTGAGTTATCTTTCCTTCGCCCACAAGTTGAAGCCTATGAAAATCAGCAGCGGTTGTTGAGTCTTGAACACCACCAATTAAGACTTGAAATTGCTGTAAGAGAAAAACAAAGGATACTTCAAGAAGTTGAGATTGAGAATAAGAAAGCTGAGGTGAATAGGCTGAGGGAGCTTCAGGAGAAACTAGTTTCTGAAGCACAAAACAAGAGGTTTGTGTATTAA

Protein sequence

>MsaT014448.1

MREENAKKINFLSSKKDKLCAVKSTVSFKPSSSMAPTKLMSSASSSFQVWKNALQKKSHDVEGTSSKINIVENPILDQAKKNIINLSFATPDAAGVNLINTNQNRESRAGKSICLDHNLGKTEEDPKILKRIEANRAAAQKSRLKKLDYIADLEQMEKYLQAELSFLRPQVEAYENQQRLLSLEHHQLRLEIAVREKQRILQEVEIENKKAEVNRLRELQEKLVSEAQNKRFVY*