AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014458


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014458 MtrunA17_Chr3g0105791 93.007 143 10 0 29 171 1 143 2.39e-96 275
MsaG014458 MtrunA17_Chr4g0031421 90.210 143 14 0 29 171 1 143 1.40e-91 263
MsaG014458 MtrunA17_Chr3g0107461 78.261 161 35 0 1 161 1 161 4.37e-89 257
MsaG014458 MtrunA17_Chr3g0107381 53.179 173 76 3 1 170 1 171 9.15e-58 180
MsaG014458 MtrunA17_Chr2g0283941 46.154 169 91 0 1 169 1 169 3.99e-49 159
MsaG014458 MtrunA17_Chr3g0110131 50.000 158 78 1 1 158 1 157 1.99e-44 146
MsaG014458 MtrunA17_Chr5g0432691 41.875 160 86 2 1 157 1 156 9.98e-42 137
MsaG014458 MtrunA17_Chr4g0014321 40.000 165 99 0 1 165 1 165 4.34e-41 137
MsaG014458 MtrunA17_Chr8g0351751 41.317 167 96 1 1 165 1 167 5.26e-40 135
MsaG014458 MtrunA17_Chr2g0296861 45.806 155 83 1 1 155 1 154 9.46e-39 129
MsaG014458 MtrunA17_Chr3g0106501 47.407 135 71 0 29 163 1 135 2.09e-38 128
MsaG014458 MtrunA17_Chr4g0014031 42.424 165 95 0 1 165 1 165 5.40e-37 127
MsaG014458 MtrunA17_Chr5g0420351 41.772 158 90 2 1 158 1 156 8.38e-37 124
MsaG014458 MtrunA17_Chr1g0188681 42.138 159 90 2 1 159 1 157 8.51e-35 119
MsaG014458 MtrunA17_Chr4g0013041 36.842 171 105 2 1 170 1 169 1.03e-34 120
MsaG014458 MtrunA17_Chr4g0014131 41.818 165 96 0 1 165 1 165 1.13e-34 121
MsaG014458 MtrunA17_Chr3g0107331 40.000 155 92 1 3 157 2 155 8.61e-34 117
MsaG014458 MtrunA17_Chr3g0106421 41.844 141 82 0 29 169 1 141 1.38e-33 118
MsaG014458 MtrunA17_Chr2g0296831 48.718 117 60 0 1 117 1 117 1.72e-32 112
MsaG014458 MtrunA17_Chr4g0014121 38.994 159 97 0 1 159 29 187 1.53e-31 112
MsaG014458 MtrunA17_Chr1g0197461 39.474 152 90 2 3 154 2 151 2.44e-31 110
MsaG014458 MtrunA17_Chr4g0012761 41.509 159 91 2 1 159 1 157 2.62e-31 110
MsaG014458 MtrunA17_Chr1g0193311 41.139 158 91 2 1 158 1 156 2.63e-31 110
MsaG014458 MtrunA17_Chr1g0188661 41.509 159 91 2 1 159 1 157 5.77e-31 109
MsaG014458 MtrunA17_Chr3g0107361 38.710 155 94 1 3 157 2 155 6.30e-31 109
MsaG014458 MtrunA17_Chr7g0218151 40.881 159 92 2 1 159 1 157 1.78e-30 108
MsaG014458 MtrunA17_Chr1g0188651 40.994 161 91 2 1 161 1 157 5.59e-30 107
MsaG014458 MtrunA17_Chr3g0107351 37.419 155 96 1 3 157 2 155 3.12e-29 105
MsaG014458 MtrunA17_Chr1g0197321 37.013 154 96 1 1 154 1 153 3.86e-28 102
MsaG014458 MtrunA17_Chr3g0107341 37.419 155 96 1 3 157 2 155 4.94e-28 102
MsaG014458 MtrunA17_Chr1g0197261 36.538 156 93 4 1 154 1 152 1.60e-26 98.6
MsaG014458 MtrunA17_Chr4g0008701 31.288 163 95 5 1 154 1 155 7.56e-17 77.0
MsaG014458 MtrunA17_Chr7g0267601 31.034 145 82 4 1 139 1 133 2.42e-16 75.5
MsaG014458 MtrunA17_Chr3g0128721 32.323 99 67 0 5 103 8 106 6.00e-14 68.6
MsaG014458 MtrunA17_Chr6g0457851 26.875 160 102 4 1 154 11 161 8.76e-14 67.8
MsaG014458 MtrunA17_Chr6g0449851 28.750 160 99 4 1 154 1 151 4.02e-12 63.2
MsaG014458 MtrunA17_Chr1g0212151 29.915 117 75 3 1 111 1 116 5.70e-12 62.8
MsaG014458 MtrunA17_Chr6g0449861 28.750 160 99 4 1 154 9 159 6.57e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014458 AT5G48670.1 50.955 157 72 3 1 155 1 154 3.91e-40 138
MsaG014458 AT3G05860.2 42.763 152 82 2 1 150 1 149 1.55e-32 115
MsaG014458 AT3G05860.3 39.535 172 99 2 1 170 1 169 3.93e-32 115
MsaG014458 AT3G05860.1 39.535 172 99 2 1 170 1 169 5.15e-32 115
MsaG014458 AT1G65330.1 41.176 153 89 1 3 155 2 153 1.59e-31 115
MsaG014458 AT1G65300.1 39.623 159 94 2 5 163 4 160 5.36e-29 108
MsaG014458 AT5G27810.1 52.809 89 42 0 29 117 1 89 5.59e-29 103
MsaG014458 AT5G26650.1 36.306 157 91 2 5 157 3 154 5.36e-27 104
MsaG014458 AT1G31630.1 37.333 150 90 3 3 151 2 148 2.77e-26 102
MsaG014458 AT5G27960.1 44.954 109 60 0 5 113 3 111 3.07e-26 102
MsaG014458 AT5G26630.1 37.662 154 95 1 1 154 1 153 4.72e-25 96.7
MsaG014458 AT1G22590.2 39.823 113 68 0 1 113 1 113 5.18e-25 95.1
MsaG014458 AT1G31640.1 38.365 159 84 4 3 155 2 152 9.66e-25 99.8
MsaG014458 AT2G28700.1 39.806 103 62 0 1 103 1 103 1.48e-19 84.0
MsaG014458 AT5G06500.1 36.036 111 67 1 1 111 1 107 2.79e-19 82.0
MsaG014458 AT2G40210.1 36.634 101 64 0 1 101 1 101 6.34e-15 71.6
MsaG014458 AT5G26580.1 37.255 102 50 1 1 102 1 88 8.39e-14 68.2
MsaG014458 AT5G55690.1 36.275 102 58 3 1 95 1 102 5.14e-11 60.1
MsaG014458 AT5G55690.2 36.275 102 58 3 1 95 1 102 5.14e-11 60.1

Find 41 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCATCTTTC+TGG 0.077675 3:+73487095 None:intergenic
ATTCAAACCATAGAATTTAA+TGG 0.173791 3:-73487057 MsaT014458.1:CDS
CGAATTTCCATTAAATTCTA+TGG 0.218755 3:+73487050 None:intergenic
GAGGTTTGGCCATCAGATTC+AGG 0.258145 3:-73487252 MsaT014458.1:CDS
CGCTACCATGACTAGAAATA+AGG 0.322931 3:-73487649 None:intergenic
AAAATCATTAAGATCAGATT+TGG 0.348340 3:+73487023 None:intergenic
TCTTCTTGAGGCAAAACATT+AGG 0.355934 3:-73487155 MsaT014458.1:CDS
AATATAATGATCAATGATAT+TGG 0.368342 3:+73487077 None:intergenic
ATCAAGAGGAGGTTGGAAAT+GGG 0.388178 3:-73486936 MsaT014458.1:CDS
GATCAAGAGGAGGTTGGAAA+TGG 0.411170 3:-73486937 MsaT014458.1:CDS
GAAGTGTGATATCTAGGTTT+AGG 0.426765 3:-73487224 MsaT014458.1:CDS
GGGTCAGAAGTGTGATATCT+AGG 0.436600 3:-73487230 MsaT014458.1:CDS
TCTAGGTTTAGGAGTTTGCC+TGG 0.445921 3:-73487213 MsaT014458.1:CDS
AGAATTTGAAGGAGGTTGAT+CGG 0.478161 3:-73486984 MsaT014458.1:CDS
ACAACCCTCGAGTAGAGGTT+TGG 0.487846 3:-73487266 MsaT014458.1:CDS
TCAAGAGGAGGTTGGAAATG+GGG 0.500358 3:-73486935 MsaT014458.1:CDS
TGATCGGAAGATGAAAGAAA+TGG 0.504501 3:-73486968 MsaT014458.1:CDS
AGGTTTGGCCATCAGATTCA+GGG 0.511068 3:-73487251 MsaT014458.1:CDS
ACGAGCAGTTGAAGAAGCAA+AGG 0.512962 3:-73487125 MsaT014458.1:CDS
AAGGGAGGAAACCAGAAAGA+TGG 0.513695 3:-73487106 MsaT014458.1:CDS
GGAGGATAACATACAGAAAG+AGG 0.518632 3:-73487593 MsaT014458.1:CDS
TTTCACCTTATTTCTAGTCA+TGG 0.525434 3:+73487644 None:intergenic
ATCAAAGATCAAGAGGAGGT+TGG 0.551560 3:-73486943 MsaT014458.1:CDS
GAGGAGGTTGGAAATGGGGT+GGG 0.563227 3:-73486931 MsaT014458.1:CDS
AGAGGAGGTTGGAAATGGGG+TGG 0.565910 3:-73486932 MsaT014458.1:CDS
CGAGCAGTTGAAGAAGCAAA+GGG 0.580872 3:-73487124 MsaT014458.1:CDS
ACATGCTTCGACTCCACATA+GGG 0.581479 3:+73487308 None:intergenic
AATGGCAATCAAAGATCAAG+AGG 0.588543 3:-73486950 MsaT014458.1:CDS
TATTGATGAGAATTTGAAGG+AGG 0.592173 3:-73486992 MsaT014458.1:CDS
CACATGCTTCGACTCCACAT+AGG 0.603214 3:+73487307 None:intergenic
AATGAAATTAGCACCCTATG+TGG 0.604835 3:-73487321 MsaT014458.1:CDS
GCAAAACAACCCTCGAGTAG+AGG 0.609086 3:-73487271 MsaT014458.1:CDS
ACATAACATGTGACTCGAAG+AGG 0.616371 3:-73487614 MsaT014458.1:CDS
CTTCTGACCCCTGAATCTGA+TGG 0.624024 3:+73487243 None:intergenic
GATGGCCAAACCTCTACTCG+AGG 0.634480 3:+73487261 None:intergenic
GGCAATCAAAGATCAAGAGG+AGG 0.656411 3:-73486947 MsaT014458.1:CDS
GGTTTGGCCATCAGATTCAG+GGG 0.657992 3:-73487250 MsaT014458.1:CDS
ATGGCCAAACCTCTACTCGA+GGG 0.659257 3:+73487262 None:intergenic
TAACATGTGACTCGAAGAGG+AGG 0.659370 3:-73487611 MsaT014458.1:CDS
GCAGTTGAAGAAGCAAAGGG+AGG 0.693727 3:-73487121 MsaT014458.1:CDS
TGGATCAGGAGGTCTTCTTG+AGG 0.700337 3:-73487167 MsaT014458.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 73486924 73487664 73486924 ID=MsaG014458
Chr3 mRNA 73486924 73487664 73486924 ID=MsaT014458.1;Parent=MsaG014458
Chr3 exon 73486924 73487360 73486924 ID=MsaT014458.1.exon2;Parent=MsaT014458.1
Chr3 CDS 73486924 73487360 73486924 ID=cds.MsaT014458.1;Parent=MsaT014458.1
Chr3 exon 73487586 73487664 73487586 ID=MsaT014458.1.exon1;Parent=MsaT014458.1
Chr3 CDS 73487586 73487664 73487586 ID=cds.MsaT014458.1;Parent=MsaT014458.1
Gene Sequence

>MsaT014458.1

ATGACTAGAAATAAGGTGAAACTAGCATACATAACATGTGACTCGAAGAGGAGGATAACATACAGAAAGAGGAAAAATGGAATTATGAAGAAGATTAATGAAATTAGCACCCTATGTGGAGTCGAAGCATGTGCAATAATTTCAGAGCAAAACAACCCTCGAGTAGAGGTTTGGCCATCAGATTCAGGGGTCAGAAGTGTGATATCTAGGTTTAGGAGTTTGCCTGGACATGAACAAAGTAAAAAGATGGTGGATCAGGAGGTCTTCTTGAGGCAAAACATTAGGAAAGTCTACGAGCAGTTGAAGAAGCAAAGGGAGGAAACCAGAAAGATGGAAATGACCAATATCATTGATCATTATATTCAAACCATAGAATTTAATGGAAATTCGATGACCAAATCTGATCTTAATGATTTTTCATCTTTTATTGATGAGAATTTGAAGGAGGTTGATCGGAAGATGAAAGAAATGGCAATCAAAGATCAAGAGGAGGTTGGAAATGGGGTGGGATTATGA

Protein sequence

>MsaT014458.1

MTRNKVKLAYITCDSKRRITYRKRKNGIMKKINEISTLCGVEACAIISEQNNPRVEVWPSDSGVRSVISRFRSLPGHEQSKKMVDQEVFLRQNIRKVYEQLKKQREETRKMEMTNIIDHYIQTIEFNGNSMTKSDLNDFSSFIDENLKEVDRKMKEMAIKDQEEVGNGVGL*