AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014510 MtrunA17_Chr3g0106501 84.516 155 24 0 29 183 1 155 1.02e-84 246
MsaG014510 MtrunA17_Chr3g0107381 50.323 155 76 1 1 155 1 154 2.94e-49 159
MsaG014510 MtrunA17_Chr3g0107461 46.875 160 85 0 1 160 1 160 1.44e-44 145
MsaG014510 MtrunA17_Chr2g0283941 43.636 165 93 0 1 165 1 165 2.29e-42 142
MsaG014510 MtrunA17_Chr3g0106421 47.482 139 73 0 29 167 1 139 9.35e-40 134
MsaG014510 MtrunA17_Chr4g0014321 39.024 164 100 0 1 164 1 164 3.20e-38 130
MsaG014510 MtrunA17_Chr3g0107331 42.593 162 86 3 3 161 2 159 3.08e-37 126
MsaG014510 MtrunA17_Chr3g0110131 42.038 157 90 1 1 157 1 156 1.08e-36 127
MsaG014510 MtrunA17_Chr3g0107361 42.038 157 88 2 3 158 2 156 2.21e-36 124
MsaG014510 MtrunA17_Chr3g0107351 42.675 157 87 2 3 158 2 156 4.78e-36 123
MsaG014510 MtrunA17_Chr8g0351751 40.244 164 96 1 1 162 1 164 3.26e-34 121
MsaG014510 MtrunA17_Chr4g0031421 43.382 136 77 0 29 164 1 136 1.38e-33 116
MsaG014510 MtrunA17_Chr4g0013041 37.662 154 94 1 1 154 1 152 3.94e-33 117
MsaG014510 MtrunA17_Chr3g0105791 43.382 136 77 0 29 164 1 136 1.09e-32 114
MsaG014510 MtrunA17_Chr3g0107341 40.764 157 90 2 3 158 2 156 2.79e-32 113
MsaG014510 MtrunA17_Chr5g0432691 37.013 154 96 1 1 154 1 153 1.25e-31 112
MsaG014510 MtrunA17_Chr4g0014031 39.873 158 95 0 1 158 1 158 6.60e-31 112
MsaG014510 MtrunA17_Chr1g0197321 41.615 161 87 4 1 158 1 157 2.14e-29 106
MsaG014510 MtrunA17_Chr4g0014131 37.654 162 101 0 1 162 1 162 1.28e-26 101
MsaG014510 MtrunA17_Chr5g0420351 36.875 160 93 3 1 157 1 155 2.91e-26 98.2
MsaG014510 MtrunA17_Chr2g0296861 36.943 157 94 2 1 155 1 154 4.01e-26 97.8
MsaG014510 MtrunA17_Chr2g0296831 43.860 114 64 0 1 114 1 114 4.35e-26 96.7
MsaG014510 MtrunA17_Chr1g0188681 35.714 154 97 2 1 154 1 152 1.04e-25 96.7
MsaG014510 MtrunA17_Chr1g0197461 38.994 159 89 5 3 158 2 155 3.52e-25 95.5
MsaG014510 MtrunA17_Chr4g0014121 35.484 155 100 0 1 155 29 183 8.25e-25 95.1
MsaG014510 MtrunA17_Chr1g0197261 34.177 158 102 2 1 158 1 156 2.73e-24 93.2
MsaG014510 MtrunA17_Chr1g0188651 38.312 154 91 2 1 154 1 150 3.09e-23 90.5
MsaG014510 MtrunA17_Chr1g0193311 36.943 157 97 2 1 157 1 155 1.15e-22 89.0
MsaG014510 MtrunA17_Chr1g0188661 34.416 154 99 2 1 154 1 152 9.67e-21 84.0
MsaG014510 MtrunA17_Chr7g0218151 34.416 154 99 2 1 154 1 152 1.36e-20 83.6
MsaG014510 MtrunA17_Chr4g0012761 34.416 154 99 2 1 154 1 152 5.57e-19 79.3
MsaG014510 MtrunA17_Chr7g0267601 29.060 117 83 0 3 119 6 122 7.89e-17 77.4
MsaG014510 MtrunA17_Chr4g0008701 30.769 117 81 0 3 119 6 122 2.15e-16 76.3
MsaG014510 MtrunA17_Chr6g0457851 32.759 116 71 3 1 110 11 125 2.26e-15 72.8
MsaG014510 MtrunA17_Chr1g0212151 31.897 116 72 3 1 110 1 115 1.22e-14 70.5
MsaG014510 MtrunA17_Chr6g0449861 31.858 113 70 3 1 107 9 120 3.56e-14 69.3
MsaG014510 MtrunA17_Chr4g0059631 34.259 108 67 3 1 105 1 107 4.18e-14 68.2
MsaG014510 MtrunA17_Chr3g0128721 24.359 156 117 1 3 158 6 160 5.46e-13 66.2
MsaG014510 MtrunA17_Chr6g0449851 30.088 113 72 3 1 107 1 112 7.00e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014510 AT3G05860.2 36.601 153 92 2 1 151 1 150 1.70e-27 103
MsaG014510 AT5G48670.1 39.623 159 91 2 1 157 1 156 3.84e-27 104
MsaG014510 AT3G05860.3 36.364 154 93 2 1 152 1 151 5.52e-27 102
MsaG014510 AT3G05860.1 36.364 154 93 2 1 152 1 151 6.22e-27 102
MsaG014510 AT1G22590.2 32.335 167 97 2 1 161 1 157 2.70e-24 93.6
MsaG014510 AT1G65300.1 39.130 115 70 0 3 117 2 116 4.24e-24 95.9
MsaG014510 AT1G65330.1 32.317 164 108 2 3 165 2 163 5.00e-24 95.9
MsaG014510 AT5G26650.1 37.719 114 71 0 4 117 2 115 3.84e-22 92.0
MsaG014510 AT2G28700.1 32.258 155 98 2 1 152 1 151 4.20e-22 91.3
MsaG014510 AT5G27960.1 36.842 114 72 0 4 117 2 115 5.21e-22 90.9
MsaG014510 AT5G06500.1 33.333 144 79 3 1 142 1 129 1.85e-21 88.2
MsaG014510 AT1G31630.1 35.652 115 73 1 3 117 2 115 5.92e-20 85.9
MsaG014510 AT5G27810.1 42.697 89 51 0 29 117 1 89 5.95e-19 78.6
MsaG014510 AT5G26630.1 33.742 163 95 3 1 157 1 156 3.87e-18 79.0
MsaG014510 AT1G31640.1 34.783 115 74 1 3 117 2 115 3.84e-17 78.6
MsaG014510 AT2G40210.1 37.000 100 63 0 1 100 1 100 4.73e-16 75.1
MsaG014510 AT5G26580.1 32.479 117 65 2 1 117 1 103 5.58e-15 72.0
MsaG014510 AT1G77980.1 30.065 153 88 4 1 152 1 135 2.22e-11 61.6
MsaG014510 AT2G03710.3 42.254 71 41 0 1 71 1 71 2.42e-11 60.1
MsaG014510 AT5G13790.1 42.466 73 40 1 1 73 1 71 3.79e-11 60.5
MsaG014510 AT5G13790.2 42.466 73 40 1 1 73 1 71 3.83e-11 60.8
MsaG014510 AT1G22130.1 38.554 83 50 1 1 83 1 82 5.01e-11 60.8
MsaG014510 AT2G03710.1 42.254 71 41 0 1 71 1 71 6.68e-11 59.7
MsaG014510 AT2G03710.2 42.254 71 41 0 1 71 1 71 6.96e-11 59.7

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACCAAAAGTTGGATTTAA+TGG 0.132212 3:-74138212 MsaT014510.1:CDS
AATCCATTAAATCCAACTTT+TGG 0.339004 3:+74138209 None:intergenic
ATCAAGTCCGTAACTTCATT+AGG 0.342103 3:+74138256 None:intergenic
GAAGCTAGATAAAATTCATT+TGG 0.361908 3:+74138139 None:intergenic
GAGGTTTGGCCTTCTCCAAC+TGG 0.364214 3:-74138496 MsaT014510.1:CDS
GAGCATCCTTTGTGGTATAG+AGG 0.426750 3:-74138557 MsaT014510.1:CDS
ACCATCATGAAGTTGAGGTT+TGG 0.438425 3:-74138510 MsaT014510.1:CDS
AACGAATTGAGCATCCTTTG+TGG 0.463741 3:-74138565 MsaT014510.1:CDS
CAATTGAATCCTCTTGAGCT+TGG 0.465024 3:+74138185 None:intergenic
GTCCGTAACTTCATTAGGAT+TGG 0.474726 3:+74138261 None:intergenic
CTTGATTAATGAGAAATTGA+AGG 0.475026 3:-74138239 MsaT014510.1:CDS
GACCAATCCTAATGAAGTTA+CGG 0.478007 3:-74138263 MsaT014510.1:CDS
GGAAAACAAGAAGAAAGAAA+TGG 0.479640 3:-74138344 MsaT014510.1:CDS
GAAGGAAGTCTACCAAAAGT+TGG 0.485133 3:-74138221 MsaT014510.1:CDS
TGAAAACCATCATGAAGTTG+AGG 0.549600 3:-74138515 MsaT014510.1:CDS
GCCAAACCTCAACTTCATGA+TGG 0.552073 3:+74138509 None:intergenic
ATCATCAAAATTAAATTCAG+TGG 0.561119 3:+74138294 None:intergenic
CACTACTATGGGCAGAAGGA+AGG 0.561138 3:-74140780 None:intergenic
AGATCAGTATCACAAACTAA+AGG 0.570022 3:-74138368 MsaT014510.1:CDS
GAGTAGTAAGTGTGTTGTCA+AGG 0.577943 3:-74138474 MsaT014510.1:CDS
CTTACTACTCCAGTTGGAGA+AGG 0.592421 3:+74138487 None:intergenic
TCAGTATCACAAACTAAAGG+TGG 0.593746 3:-74138365 MsaT014510.1:CDS
AACACACTTACTACTCCAGT+TGG 0.596457 3:+74138481 None:intergenic
TACTATGGGCAGAAGGAAGG+TGG 0.600474 3:-74140777 None:intergenic
TCATCACTACTATGGGCAGA+AGG 0.612227 3:-74140784 None:intergenic
AATGGATTTCCAAGCTCAAG+AGG 0.613698 3:-74138194 MsaT014510.1:CDS
TAGTCAACAACTCAAAGCGG+AGG 0.677810 3:-74140742 MsaT014510.1:CDS
CACAAGCCTCTATACCACAA+AGG 0.687096 3:+74138551 None:intergenic
ACATAGTCAACAACTCAAAG+CGG 0.715101 3:-74140745 MsaT014510.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 74138132 74140795 74138132 ID=MsaG014510
Chr3 mRNA 74138132 74140795 74138132 ID=MsaT014510.1;Parent=MsaG014510
Chr3 exon 74138132 74138604 74138132 ID=MsaT014510.1.exon2;Parent=MsaT014510.1
Chr3 CDS 74138132 74138604 74138132 ID=cds.MsaT014510.1;Parent=MsaT014510.1
Chr3 exon 74140717 74140795 74140717 ID=MsaT014510.1.exon1;Parent=MsaT014510.1
Chr3 CDS 74140717 74140795 74140717 ID=cds.MsaT014510.1;Parent=MsaT014510.1
Gene Sequence

>MsaT014510.1

ATGGGCAGAAGGAAGGTGGAACTCGCATACATAGTCAACAACTCAAAGCGGAGGTTAACATTCAGAACAAGAAAAAATGGGTTATTGAAGATGATCAACGAATTGAGCATCCTTTGTGGTATAGAGGCTTGTGCAATCATTTTTGATGAAAACCATCATGAAGTTGAGGTTTGGCCTTCTCCAACTGGAGTAGTAAGTGTGTTGTCAAGGTTCTCGAATTTGTCCGAACATGAAAAAAGAACAAGGATGCTGGATTTAGAGAGCTACTTGAAACAAAGAATTGAGAAAGCTCAAGATCAGTATCACAAACTAAAGGTGGAAAACAAGAAGAAAGAAATGGCTGAATATATAAACAAATATATGTGTGCCACTGAATTTAATTTTGATGATGCTCACTTGACCAATCCTAATGAAGTTACGGACTTGATTAATGAGAAATTGAAGGAAGTCTACCAAAAGTTGGATTTAATGGATTTCCAAGCTCAAGAGGATTCAATTGATTTTGATGATATTGATTTCGATCCAAATGAATTTTATCTAGCTTCTCCATAA
Protein sequence

>MsaT014510.1

MGRRKVELAYIVNNSKRRLTFRTRKNGLLKMINELSILCGIEACAIIFDENHHEVEVWPSPTGVVSVLSRFSNLSEHEKRTRMLDLESYLKQRIEKAQDQYHKLKVENKKKEMAEYINKYMCATEFNFDDAHLTNPNEVTDLINEKLKEVYQKLDLMDFQAQEDSIDFDDIDFDPNEFYLASP*