Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014515 | XP_013460360.1 | 96.460 | 226 | 8 | 0 | 1 | 226 | 1 | 226 | 4.22e-160 | 452 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014515 | sp|Q96S44|PRPK_HUMAN | 49.091 | 220 | 109 | 2 | 10 | 226 | 34 | 253 | 4.45e-64 | 202 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014515 | tr|I3SWE5|I3SWE5_MEDTR | 96.460 | 226 | 8 | 0 | 1 | 226 | 1 | 226 | 2.02e-160 | 452 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG001810 | MsaG014515 | 0.815400 | 1.632411e-51 | 3.187686e-49 |
| MsaG002481 | MsaG014515 | 0.813294 | 4.742578e-51 | 8.783006e-49 |
| MsaG002871 | MsaG014515 | 0.814956 | 2.046295e-51 | 3.951291e-49 |
| MsaG003662 | MsaG014515 | 0.816213 | 1.077297e-51 | 2.147602e-49 |
| MsaG004070 | MsaG014515 | 0.817249 | 6.325510e-52 | 1.295031e-49 |
| MsaG004096 | MsaG014515 | 0.855249 | 1.385086e-61 | 9.028863e-59 |
| MsaG004773 | MsaG014515 | 0.827066 | 3.425152e-54 | 9.124097e-52 |
| MsaG004856 | MsaG014515 | 0.816557 | 9.029979e-52 | 1.815986e-49 |
| MsaG006033 | MsaG014515 | 0.826149 | 5.655747e-54 | 1.468730e-51 |
| MsaG006657 | MsaG014515 | 0.821378 | 7.326777e-53 | 1.671255e-50 |
| MsaG006765 | MsaG014515 | 0.820098 | 1.437889e-52 | 3.170755e-50 |
| MsaG011484 | MsaG014515 | 0.831329 | 3.202961e-55 | 9.629882e-53 |
| MsaG013211 | MsaG014515 | 0.852440 | 8.840141e-61 | 5.215076e-58 |
| MsaG013340 | MsaG014515 | 0.962122 | 6.147783e-120 | 3.711372e-114 |
| MsaG014515 | MsaG014848 | 0.800481 | 2.362187e-48 | 3.228700e-46 |
| MsaG014515 | MsaG015445 | 0.811576 | 1.121090e-50 | 1.989676e-48 |
| MsaG014515 | MsaG016304 | 0.802447 | 9.383647e-49 | 1.341127e-46 |
| MsaG014515 | MsaG016873 | 0.800063 | 2.870729e-48 | 3.886982e-46 |
| MsaG014515 | MsaG016974 | 0.811453 | 1.191841e-50 | 2.108894e-48 |
| MsaG014515 | MsaG017136 | 0.804567 | 3.426662e-49 | 5.143156e-47 |
| MsaG014515 | MsaG017396 | 0.819525 | 1.940453e-52 | 4.214429e-50 |
| MsaG014515 | MsaG017503 | 0.831234 | 3.377564e-55 | 1.012677e-52 |
| MsaG014515 | MsaG018598 | 0.813012 | 5.466076e-51 | 1.005217e-48 |
| MsaG014515 | MsaG021395 | 0.832642 | 1.522948e-55 | 4.757313e-53 |
| MsaG014515 | MsaG021556 | 0.815114 | 1.888252e-51 | 3.660582e-49 |
| MsaG014515 | MsaG022325 | 0.813592 | 4.082280e-51 | 7.617245e-49 |
| MsaG014515 | MsaG026934 | 0.805196 | 2.535223e-49 | 3.861176e-47 |
| MsaG014515 | MsaG027006 | 0.832127 | 2.040210e-55 | 6.279047e-53 |
| MsaG014515 | MsaG027590 | 0.812975 | 5.568760e-51 | 1.023138e-48 |
| MsaG014515 | MsaG027950 | 0.834718 | 4.640020e-56 | 1.541259e-53 |
| MsaG014515 | MsaG028619 | 0.805376 | 2.326313e-49 | 3.557742e-47 |
| MsaG014515 | MsaG028661 | 0.814228 | 2.960419e-51 | 5.612727e-49 |
| MsaG014515 | MsaG029464 | 0.840130 | 1.934401e-57 | 7.582143e-55 |
| MsaG014515 | MsaG030590 | 0.805837 | 1.863106e-49 | 2.880247e-47 |
| MsaG014515 | MsaG039781 | 0.818971 | 2.591365e-52 | 5.547104e-50 |
| MsaG014515 | MsaG044546 | 0.800908 | 1.935004e-48 | 2.670339e-46 |
| MsaG014515 | MsaG044916 | 0.817769 | 4.835198e-52 | 1.003229e-49 |
| MsaG014515 | MsaG045104 | 0.808808 | 4.401516e-50 | 7.303549e-48 |
| MsaG014515 | MsaG045331 | 0.807959 | 6.665299e-50 | 1.083614e-47 |
| MsaG014515 | MsaG045986 | 0.806007 | 1.717109e-49 | 2.665045e-47 |
| MsaG014515 | MsaG046536 | 0.817801 | 4.757191e-52 | 9.878455e-50 |
| MsaG014515 | MsaG046497 | 0.827640 | 2.499146e-54 | 6.765572e-52 |
| MsaG014515 | MsaG046660 | 0.806033 | 1.695371e-49 | 2.633041e-47 |
| MsaG014515 | MsaG046780 | 0.805080 | 2.681460e-49 | 4.072776e-47 |
| MsaG014515 | MsaG012667 | 0.810717 | 1.717998e-50 | 2.985559e-48 |
| MsaG014515 | MsaG013546 | 0.800026 | 2.921131e-48 | 3.951888e-46 |
| MsaG014515 | MsaG031638 | 0.825018 | 1.045158e-53 | 2.630438e-51 |
| MsaG014515 | MsaG036116 | 0.802004 | 1.156207e-48 | 1.635750e-46 |
| MsaG014515 | MsaG038591 | 0.807174 | 9.761735e-50 | 1.557511e-47 |
| MsaG014515 | MsaG037725 | 0.803886 | 4.742766e-49 | 7.006830e-47 |
| MsaG014515 | MsaG037447 | 0.811013 | 1.483268e-50 | 2.596361e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014515 | MtrunA17_Chr3g0106561 | 96.460 | 226 | 8 | 0 | 1 | 226 | 1 | 226 | 3.88e-164 | 452 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014515 | AT5G26110.3 | 78.761 | 226 | 48 | 0 | 1 | 226 | 1 | 226 | 7.84e-134 | 375 |
| MsaG014515 | AT5G26110.1 | 78.761 | 226 | 48 | 0 | 1 | 226 | 1 | 226 | 7.84e-134 | 375 |
| MsaG014515 | AT5G26110.2 | 79.012 | 162 | 34 | 0 | 65 | 226 | 2 | 163 | 7.13e-95 | 275 |
| MsaG014515 | AT1G08120.1 | 73.684 | 57 | 15 | 0 | 1 | 57 | 5 | 61 | 4.60e-22 | 87.0 |
Find 43 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTACAGACATCCGATTT+TGG | 0.315543 | 3:-74163688 | MsaT014515.1:CDS |
| AAAGATGTATTTCTTGAATT+CGG | 0.327087 | 3:-74163111 | MsaT014515.1:CDS |
| CACGATGGTGGTCTTGTTCA+TGG | 0.374963 | 3:-74163018 | MsaT014515.1:CDS |
| GGCAAGATTGCATCTCAAAT+TGG | 0.384705 | 3:-74163060 | MsaT014515.1:CDS |
| CAACCAAGGCAAGGCGACTT+GGG | 0.419060 | 3:-74163212 | MsaT014515.1:CDS |
| GTGTAGGTTCTTATTGACTT+TGG | 0.438221 | 3:-74162838 | MsaT014515.1:intron |
| ACAACCAAGGCAAGGCGACT+TGG | 0.438223 | 3:-74163213 | MsaT014515.1:CDS |
| GTCAACTTAGCATCCAAAAT+CGG | 0.456518 | 3:+74163675 | None:intergenic |
| AAATCGACGGCTTTATCTTC+TGG | 0.462118 | 3:+74162793 | None:intergenic |
| ACCACTGTTTCGATGATTTG+CGG | 0.463118 | 3:+74161914 | None:intergenic |
| AACCCCAAGTCGCCTTGCCT+TGG | 0.476565 | 3:+74163209 | None:intergenic |
| AAAGAATGATACTGATCAGT+TGG | 0.526434 | 3:-74162968 | MsaT014515.1:intron |
| TTAACATTTGAATTTGTCGA+TGG | 0.536270 | 3:-74163144 | MsaT014515.1:CDS |
| TACCCCTGTGTTATATGCTG+TGG | 0.540309 | 3:-74163184 | MsaT014515.1:CDS |
| GTTTCGATGCATTCTTCATG+TGG | 0.542927 | 3:-74162748 | MsaT014515.1:CDS |
| AGAAATACATCTTTGACTGA+AGG | 0.543009 | 3:+74163120 | None:intergenic |
| ATGAAGAATGCATCGAAACA+AGG | 0.554852 | 3:+74162752 | None:intergenic |
| GTCTGCAAGCTTGTTCATTG+TGG | 0.559060 | 3:+74161888 | None:intergenic |
| TTCTAAAACATACAAATCGA+CGG | 0.561624 | 3:+74162780 | None:intergenic |
| AATGTTAAAGTGTGCAACAC+AGG | 0.565875 | 3:+74163159 | None:intergenic |
| TTTCTTGAATTCGGATCATG+TGG | 0.569490 | 3:-74163102 | MsaT014515.1:CDS |
| GGACGAAAGAGGACCATGGT+TGG | 0.570787 | 3:-74161770 | MsaT014515.1:CDS |
| GGCATCAATGAAGAGCGGTT+AGG | 0.593530 | 3:-74163081 | MsaT014515.1:CDS |
| CGAGGTGCACAACCAAGGCA+AGG | 0.594894 | 3:-74163221 | MsaT014515.1:CDS |
| AACCAAGGCAAGGCGACTTG+GGG | 0.602601 | 3:-74163211 | MsaT014515.1:CDS |
| TGCGACAAAGAGGACGAAAG+AGG | 0.603308 | 3:-74161781 | MsaT014515.1:CDS |
| TTGTTTGATGAGAATGAGAG+AGG | 0.606756 | 3:+74164179 | None:intergenic |
| GGATCCACAGCATATAACAC+AGG | 0.609862 | 3:+74163180 | None:intergenic |
| TGAAGAATGCATCGAAACAA+GGG | 0.628508 | 3:+74162753 | None:intergenic |
| GGAGGCGAGGTGCACAACCA+AGG | 0.637554 | 3:-74163226 | MsaT014515.1:CDS |
| GATCCACAGCATATAACACA+GGG | 0.645198 | 3:+74163181 | None:intergenic |
| CAACTTGTAGTGCGACAAAG+AGG | 0.647624 | 3:-74161791 | MsaT014515.1:intron |
| ACAATGAACAAGCTTGCAGA+CGG | 0.653580 | 3:-74161886 | MsaT014515.1:intron |
| ATTGCAAAGTTACACGATGG+TGG | 0.654578 | 3:-74163030 | MsaT014515.1:CDS |
| GCATTCTTCATGTGGTAATG+TGG | 0.655251 | 3:-74162740 | MsaT014515.1:intron |
| TCTCTCATTCTCATCAAACA+AGG | 0.656080 | 3:-74164177 | MsaT014515.1:CDS |
| GACACTCAAGCGCTTAAATG+CGG | 0.662510 | 3:-74163655 | MsaT014515.1:intron |
| ACCGCAAATCATCGAAACAG+TGG | 0.688110 | 3:-74161915 | MsaT014515.1:CDS |
| AACTCAGAATCATCCAACCA+TGG | 0.691341 | 3:+74161757 | None:intergenic |
| CATGTGGCATCAATGAAGAG+CGG | 0.710732 | 3:-74163086 | MsaT014515.1:CDS |
| AAGAGGACGAAAGAGGACCA+TGG | 0.717008 | 3:-74161774 | MsaT014515.1:CDS |
| GTAATTGCAAAGTTACACGA+TGG | 0.807657 | 3:-74163033 | MsaT014515.1:CDS |
| ATCCACAGCATATAACACAG+GGG | 0.823142 | 3:+74163182 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 74161766 | 74164229 | 74161766 | ID=MsaG014515 |
| Chr3 | mRNA | 74161766 | 74164229 | 74161766 | ID=MsaT014515.1;Parent=MsaG014515 |
| Chr3 | exon | 74161766 | 74161803 | 74161766 | ID=MsaT014515.1.exon6;Parent=MsaT014515.1 |
| Chr3 | CDS | 74161766 | 74161803 | 74161766 | ID=cds.MsaT014515.1;Parent=MsaT014515.1 |
| Chr3 | exon | 74161887 | 74161959 | 74161887 | ID=MsaT014515.1.exon5;Parent=MsaT014515.1 |
| Chr3 | CDS | 74161887 | 74161959 | 74161887 | ID=cds.MsaT014515.1;Parent=MsaT014515.1 |
| Chr3 | exon | 74162741 | 74162854 | 74162741 | ID=MsaT014515.1.exon4;Parent=MsaT014515.1 |
| Chr3 | CDS | 74162741 | 74162854 | 74162741 | ID=cds.MsaT014515.1;Parent=MsaT014515.1 |
| Chr3 | exon | 74162969 | 74163247 | 74162969 | ID=MsaT014515.1.exon3;Parent=MsaT014515.1 |
| Chr3 | CDS | 74162969 | 74163247 | 74162969 | ID=cds.MsaT014515.1;Parent=MsaT014515.1 |
| Chr3 | exon | 74163656 | 74163769 | 74163656 | ID=MsaT014515.1.exon2;Parent=MsaT014515.1 |
| Chr3 | CDS | 74163656 | 74163769 | 74163656 | ID=cds.MsaT014515.1;Parent=MsaT014515.1 |
| Chr3 | exon | 74164167 | 74164229 | 74164167 | ID=MsaT014515.1.exon1;Parent=MsaT014515.1 |
| Chr3 | CDS | 74164167 | 74164229 | 74164167 | ID=cds.MsaT014515.1;Parent=MsaT014515.1 |
| Gene Sequence |
| Protein sequence |
