AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014515


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014515 MtrunA17_Chr3g0106561 96.460 226 8 0 1 226 1 226 3.88e-164 452
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014515 AT5G26110.3 78.761 226 48 0 1 226 1 226 7.84e-134 375
MsaG014515 AT5G26110.1 78.761 226 48 0 1 226 1 226 7.84e-134 375
MsaG014515 AT5G26110.2 79.012 162 34 0 65 226 2 163 7.13e-95 275
MsaG014515 AT1G08120.1 73.684 57 15 0 1 57 5 61 4.60e-22 87.0

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGTACAGACATCCGATTT+TGG 0.315543 3:-74163688 MsaT014515.1:CDS
AAAGATGTATTTCTTGAATT+CGG 0.327087 3:-74163111 MsaT014515.1:CDS
CACGATGGTGGTCTTGTTCA+TGG 0.374963 3:-74163018 MsaT014515.1:CDS
GGCAAGATTGCATCTCAAAT+TGG 0.384705 3:-74163060 MsaT014515.1:CDS
CAACCAAGGCAAGGCGACTT+GGG 0.419060 3:-74163212 MsaT014515.1:CDS
GTGTAGGTTCTTATTGACTT+TGG 0.438221 3:-74162838 MsaT014515.1:intron
ACAACCAAGGCAAGGCGACT+TGG 0.438223 3:-74163213 MsaT014515.1:CDS
GTCAACTTAGCATCCAAAAT+CGG 0.456518 3:+74163675 None:intergenic
AAATCGACGGCTTTATCTTC+TGG 0.462118 3:+74162793 None:intergenic
ACCACTGTTTCGATGATTTG+CGG 0.463118 3:+74161914 None:intergenic
AACCCCAAGTCGCCTTGCCT+TGG 0.476565 3:+74163209 None:intergenic
AAAGAATGATACTGATCAGT+TGG 0.526434 3:-74162968 MsaT014515.1:intron
TTAACATTTGAATTTGTCGA+TGG 0.536270 3:-74163144 MsaT014515.1:CDS
TACCCCTGTGTTATATGCTG+TGG 0.540309 3:-74163184 MsaT014515.1:CDS
GTTTCGATGCATTCTTCATG+TGG 0.542927 3:-74162748 MsaT014515.1:CDS
AGAAATACATCTTTGACTGA+AGG 0.543009 3:+74163120 None:intergenic
ATGAAGAATGCATCGAAACA+AGG 0.554852 3:+74162752 None:intergenic
GTCTGCAAGCTTGTTCATTG+TGG 0.559060 3:+74161888 None:intergenic
TTCTAAAACATACAAATCGA+CGG 0.561624 3:+74162780 None:intergenic
AATGTTAAAGTGTGCAACAC+AGG 0.565875 3:+74163159 None:intergenic
TTTCTTGAATTCGGATCATG+TGG 0.569490 3:-74163102 MsaT014515.1:CDS
GGACGAAAGAGGACCATGGT+TGG 0.570787 3:-74161770 MsaT014515.1:CDS
GGCATCAATGAAGAGCGGTT+AGG 0.593530 3:-74163081 MsaT014515.1:CDS
CGAGGTGCACAACCAAGGCA+AGG 0.594894 3:-74163221 MsaT014515.1:CDS
AACCAAGGCAAGGCGACTTG+GGG 0.602601 3:-74163211 MsaT014515.1:CDS
TGCGACAAAGAGGACGAAAG+AGG 0.603308 3:-74161781 MsaT014515.1:CDS
TTGTTTGATGAGAATGAGAG+AGG 0.606756 3:+74164179 None:intergenic
GGATCCACAGCATATAACAC+AGG 0.609862 3:+74163180 None:intergenic
TGAAGAATGCATCGAAACAA+GGG 0.628508 3:+74162753 None:intergenic
GGAGGCGAGGTGCACAACCA+AGG 0.637554 3:-74163226 MsaT014515.1:CDS
GATCCACAGCATATAACACA+GGG 0.645198 3:+74163181 None:intergenic
CAACTTGTAGTGCGACAAAG+AGG 0.647624 3:-74161791 MsaT014515.1:intron
ACAATGAACAAGCTTGCAGA+CGG 0.653580 3:-74161886 MsaT014515.1:intron
ATTGCAAAGTTACACGATGG+TGG 0.654578 3:-74163030 MsaT014515.1:CDS
GCATTCTTCATGTGGTAATG+TGG 0.655251 3:-74162740 MsaT014515.1:intron
TCTCTCATTCTCATCAAACA+AGG 0.656080 3:-74164177 MsaT014515.1:CDS
GACACTCAAGCGCTTAAATG+CGG 0.662510 3:-74163655 MsaT014515.1:intron
ACCGCAAATCATCGAAACAG+TGG 0.688110 3:-74161915 MsaT014515.1:CDS
AACTCAGAATCATCCAACCA+TGG 0.691341 3:+74161757 None:intergenic
CATGTGGCATCAATGAAGAG+CGG 0.710732 3:-74163086 MsaT014515.1:CDS
AAGAGGACGAAAGAGGACCA+TGG 0.717008 3:-74161774 MsaT014515.1:CDS
GTAATTGCAAAGTTACACGA+TGG 0.807657 3:-74163033 MsaT014515.1:CDS
ATCCACAGCATATAACACAG+GGG 0.823142 3:+74163182 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 74161766 74164229 74161766 ID=MsaG014515
Chr3 mRNA 74161766 74164229 74161766 ID=MsaT014515.1;Parent=MsaG014515
Chr3 exon 74161766 74161803 74161766 ID=MsaT014515.1.exon6;Parent=MsaT014515.1
Chr3 CDS 74161766 74161803 74161766 ID=cds.MsaT014515.1;Parent=MsaT014515.1
Chr3 exon 74161887 74161959 74161887 ID=MsaT014515.1.exon5;Parent=MsaT014515.1
Chr3 CDS 74161887 74161959 74161887 ID=cds.MsaT014515.1;Parent=MsaT014515.1
Chr3 exon 74162741 74162854 74162741 ID=MsaT014515.1.exon4;Parent=MsaT014515.1
Chr3 CDS 74162741 74162854 74162741 ID=cds.MsaT014515.1;Parent=MsaT014515.1
Chr3 exon 74162969 74163247 74162969 ID=MsaT014515.1.exon3;Parent=MsaT014515.1
Chr3 CDS 74162969 74163247 74162969 ID=cds.MsaT014515.1;Parent=MsaT014515.1
Chr3 exon 74163656 74163769 74163656 ID=MsaT014515.1.exon2;Parent=MsaT014515.1
Chr3 CDS 74163656 74163769 74163656 ID=cds.MsaT014515.1;Parent=MsaT014515.1
Chr3 exon 74164167 74164229 74164167 ID=MsaT014515.1.exon1;Parent=MsaT014515.1
Chr3 CDS 74164167 74164229 74164167 ID=cds.MsaT014515.1;Parent=MsaT014515.1
Gene Sequence

>MsaT014515.1

ATGGGGACTGAAATTGAGTCAACAGATACCTCTCTCATTCTCATCAAACAAGGAGCTGAAGCTAGGGTTTTTGAGACTTCTTTTGTGGGAAGAAGGTGTGTTGTCAAAGAGAGATTCTCAAAGAAGTACAGACATCCGATTTTGGATGCTAAGTTGACACTCAAGCGCTTAAATGCGGAGGCGAGGTGCACAACCAAGGCAAGGCGACTTGGGGTTTGTACCCCTGTGTTATATGCTGTGGATCCTGTGTTGCACACTTTAACATTTGAATTTGTCGATGGACCTTCAGTCAAAGATGTATTTCTTGAATTCGGATCATGTGGCATCAATGAAGAGCGGTTAGGCAAGATTGCATCTCAAATTGGTGATGTAATTGCAAAGTTACACGATGGTGGTCTTGTTCATGGTGATTTAACAACATCAAACATGTTACTAAAGAATGATACTGATCAGTTGGTTCTTATTGACTTTGGTTTGAGCTTCACTTCAACTCTACCAGAAGATAAAGCCGTCGATTTGTATGTTTTAGAAAGAGCCCTTGTTTCGATGCATTCTTCATGTGGTAATGTGATGGATCAGATACTTGCTGCTTACCGCAAATCATCGAAACAGTGGTCATCCACAATGAACAAGCTTGCAGACGTGCGACAAAGAGGACGAAAGAGGACCATGGTTGGATGA

Protein sequence

>MsaT014515.1

MGTEIESTDTSLILIKQGAEARVFETSFVGRRCVVKERFSKKYRHPILDAKLTLKRLNAEARCTTKARRLGVCTPVLYAVDPVLHTLTFEFVDGPSVKDVFLEFGSCGINEERLGKIASQIGDVIAKLHDGGLVHGDLTTSNMLLKNDTDQLVLIDFGLSFTSTLPEDKAVDLYVLERALVSMHSSCGNVMDQILAAYRKSSKQWSSTMNKLADVRQRGRKRTMVG*