AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014586

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014586 MtrunA17_Chr3g0107331 94.410 161 9 0 1 161 1 161 3.70e-110 310
MsaG014586 MtrunA17_Chr3g0107361 79.375 160 33 0 1 160 1 160 6.25e-91 261
MsaG014586 MtrunA17_Chr3g0107351 75.625 160 39 0 1 160 1 160 3.13e-86 249
MsaG014586 MtrunA17_Chr3g0107341 75.625 160 39 0 1 160 1 160 5.12e-85 246
MsaG014586 MtrunA17_Chr3g0107381 47.059 153 81 0 2 154 3 155 5.30e-49 157
MsaG014586 MtrunA17_Chr3g0110131 53.247 154 72 0 2 155 3 156 1.28e-48 157
MsaG014586 MtrunA17_Chr8g0351751 47.853 163 82 2 2 161 3 165 6.10e-48 155
MsaG014586 MtrunA17_Chr4g0014321 42.593 162 90 2 2 161 3 163 4.39e-44 145
MsaG014586 MtrunA17_Chr4g0013041 43.671 158 84 3 3 158 4 158 3.05e-41 137
MsaG014586 MtrunA17_Chr4g0014031 47.205 161 84 1 2 161 3 163 4.03e-41 137
MsaG014586 MtrunA17_Chr2g0296831 53.782 119 55 0 2 120 3 121 1.96e-39 129
MsaG014586 MtrunA17_Chr1g0197261 43.226 155 87 1 2 156 3 156 6.29e-39 129
MsaG014586 MtrunA17_Chr1g0197461 43.312 157 88 1 1 157 1 156 2.82e-38 128
MsaG014586 MtrunA17_Chr4g0014131 46.914 162 85 1 1 161 2 163 1.44e-37 128
MsaG014586 MtrunA17_Chr4g0014121 49.020 153 77 1 1 152 30 182 1.87e-37 127
MsaG014586 MtrunA17_Chr5g0432691 43.506 154 87 0 3 156 4 157 1.94e-36 123
MsaG014586 MtrunA17_Chr5g0420351 43.506 154 86 1 2 155 3 155 8.69e-36 121
MsaG014586 MtrunA17_Chr1g0188681 44.737 152 83 1 1 152 2 152 3.19e-34 117
MsaG014586 MtrunA17_Chr1g0197321 39.744 156 92 2 2 156 3 157 6.95e-33 114
MsaG014586 MtrunA17_Chr2g0283941 41.497 147 85 1 3 148 4 150 8.44e-32 114
MsaG014586 MtrunA17_Chr7g0218151 45.223 157 85 1 2 158 3 158 1.57e-30 108
MsaG014586 MtrunA17_Chr3g0106501 44.030 134 72 2 28 159 1 133 3.89e-30 107
MsaG014586 MtrunA17_Chr3g0107461 36.842 152 95 1 2 152 3 154 2.45e-29 105
MsaG014586 MtrunA17_Chr1g0188661 45.223 157 85 1 2 158 3 158 2.45e-29 105
MsaG014586 MtrunA17_Chr1g0193311 44.156 154 85 1 2 155 3 155 3.16e-29 105
MsaG014586 MtrunA17_Chr4g0012761 42.038 157 90 1 2 158 3 158 6.28e-28 101
MsaG014586 MtrunA17_Chr1g0188651 43.671 158 86 2 1 158 2 156 8.10e-26 96.3
MsaG014586 MtrunA17_Chr2g0296861 38.667 150 92 0 3 152 4 153 8.62e-25 93.6
MsaG014586 MtrunA17_Chr3g0106421 37.594 133 82 1 28 159 1 133 1.58e-22 89.4
MsaG014586 MtrunA17_Chr4g0008701 31.788 151 102 1 2 152 6 155 4.27e-19 83.2
MsaG014586 MtrunA17_Chr3g0105791 32.558 129 86 1 28 155 1 129 6.34e-18 75.5
MsaG014586 MtrunA17_Chr7g0267601 30.921 152 104 1 2 153 6 156 9.41e-18 79.3
MsaG014586 MtrunA17_Chr4g0031421 32.558 129 86 1 28 155 1 129 6.02e-17 73.2
MsaG014586 MtrunA17_Chr3g0128721 27.389 157 114 0 2 158 6 162 1.04e-15 73.6
MsaG014586 MtrunA17_Chr6g0457851 35.455 110 64 3 2 105 13 121 2.72e-13 66.2
MsaG014586 MtrunA17_Chr6g0449861 33.636 110 66 3 2 105 11 119 2.55e-12 63.5
MsaG014586 MtrunA17_Chr6g0449851 35.714 112 61 4 2 105 3 111 4.28e-12 62.8
MsaG014586 MtrunA17_Chr1g0212151 32.727 110 67 3 2 105 3 111 1.05e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014586 AT5G48670.1 44.654 159 88 0 2 160 3 161 2.21e-37 130
MsaG014586 AT1G65300.1 39.474 152 92 0 1 152 1 152 2.24e-33 119
MsaG014586 AT1G65330.1 40.789 152 90 0 1 152 1 152 2.63e-33 119
MsaG014586 AT3G05860.2 38.776 147 90 0 2 148 3 149 9.47e-29 105
MsaG014586 AT3G05860.3 38.158 152 94 0 2 153 3 154 3.22e-28 105
MsaG014586 AT3G05860.1 38.158 152 94 0 2 153 3 154 4.47e-28 105
MsaG014586 AT5G26650.1 35.333 150 97 0 3 152 2 151 1.64e-25 100
MsaG014586 AT5G27960.1 39.831 118 71 0 3 120 2 119 4.87e-25 98.6
MsaG014586 AT5G27810.1 44.681 94 52 0 28 121 1 94 6.84e-25 93.2
MsaG014586 AT5G26630.1 38.411 151 93 0 2 152 3 153 7.19e-25 95.9
MsaG014586 AT1G31630.1 30.921 152 104 1 1 152 1 151 6.16e-22 90.5
MsaG014586 AT2G28700.1 36.975 119 75 0 2 120 3 121 2.93e-21 88.6
MsaG014586 AT1G31640.1 31.788 151 102 1 1 151 1 150 4.41e-21 89.0
MsaG014586 AT1G22590.2 29.747 158 108 1 2 159 3 157 4.71e-20 82.0
MsaG014586 AT5G26580.1 37.815 119 58 2 3 120 4 107 3.17e-18 80.5
MsaG014586 AT2G40210.1 39.394 99 60 0 2 100 3 101 6.27e-14 68.6
MsaG014586 AT5G55690.1 31.847 157 85 3 2 151 3 144 2.04e-13 66.2
MsaG014586 AT5G55690.2 31.847 157 85 3 2 151 3 144 2.04e-13 66.2
MsaG014586 AT5G06500.1 29.661 118 83 0 2 119 3 120 2.83e-11 60.1

Find 34 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTTCATCAATTGCTCTT+TGG 0.234493 3:+75559091 None:intergenic
CAAAGAGCAATTGATGAAAT+TGG 0.277019 3:-75559090 MsaT014586.1:CDS
TGGTAATCATAATCTCTAAT+TGG 0.297332 3:+75558899 None:intergenic
GAAGGTGAAACTTGCATTCA+TGG 0.348876 3:-75559369 MsaT014586.1:CDS
TGAAATTGGAGAAGAACAAT+TGG 0.354245 3:-75559076 MsaT014586.1:CDS
CTGAGGTTTGGCCTTCTCCA+TGG 0.412577 3:-75559217 MsaT014586.1:CDS
TTAACTTTGTGAGAACCCTT+TGG 0.427011 3:+75559189 None:intergenic
TTCATTCAGAGCCTGAGGTT+TGG 0.430822 3:-75559229 MsaT014586.1:CDS
TCTCCCTCAAATAGCTCTCT+TGG 0.434445 3:+75559126 None:intergenic
CTTCTCCATGGGAGGTCCAA+AGG 0.434530 3:-75559205 MsaT014586.1:CDS
ACCACCCTATGTGGGATTGA+TGG 0.443138 3:-75559275 MsaT014586.1:CDS
TGAGGTTTGGCCTTCTCCAT+GGG 0.458648 3:-75559216 MsaT014586.1:CDS
TTCTCCATGGGAGGTCCAAA+GGG 0.475139 3:-75559204 MsaT014586.1:CDS
GAAATTGGAGAAGAACAATT+GGG 0.477120 3:-75559075 MsaT014586.1:CDS
AAGAGAGCTATTTGAGGGAG+AGG 0.486973 3:-75559124 MsaT014586.1:CDS
AAGTTTCACCTTCTTTCTCA+TGG 0.490030 3:+75559379 None:intergenic
CATGGAGAAGGCCAAACCTC+AGG 0.496084 3:+75559218 None:intergenic
AATGAACTCACCACCCTATG+TGG 0.505218 3:-75559284 MsaT014586.1:CDS
GATGAAGGAGATTACTTCAA+AGG 0.505350 3:-75558946 MsaT014586.1:CDS
GGAAATTAATGAGAAGATGA+AGG 0.515316 3:-75558961 MsaT014586.1:CDS
AATTATTCAGAATTCGAGCT+AGG 0.540322 3:-75559164 MsaT014586.1:CDS
GCATCCATCAATCCCACATA+GGG 0.545713 3:+75559271 None:intergenic
GAACCAAGAGAGCTATTTGA+GGG 0.547706 3:-75559129 MsaT014586.1:CDS
AGAACCCTTTGGACCTCCCA+TGG 0.549154 3:+75559200 None:intergenic
AAGACATTGAAACAAGATCA+AGG 0.553196 3:+75559040 None:intergenic
TGAACCAAGAGAGCTATTTG+AGG 0.555069 3:-75559130 MsaT014586.1:CDS
CTTTGGACCTCCCATGGAGA+AGG 0.564387 3:+75559206 None:intergenic
CGAATTTCATTCAGAGCCTG+AGG 0.566026 3:-75559234 MsaT014586.1:CDS
CACACTGACCATGAGAAAGA+AGG 0.576636 3:-75559387 None:intergenic
CGCATCCATCAATCCCACAT+AGG 0.580138 3:+75559270 None:intergenic
GGTTTGGCCTTCTCCATGGG+AGG 0.605628 3:-75559213 MsaT014586.1:CDS
TCCATCAATCCCACATAGGG+TGG 0.620094 3:+75559274 None:intergenic
ATGAACTCACCACCCTATGT+GGG 0.624743 3:-75559283 MsaT014586.1:CDS
TCATGGTCAGTGATTCTGCA+AGG 0.648846 3:-75559352 MsaT014586.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 75558914 75559399 75558914 ID=MsaG014586
Chr3 mRNA 75558914 75559399 75558914 ID=MsaT014586.1;Parent=MsaG014586
Chr3 exon 75558914 75559399 75558914 ID=MsaT014586.1.exon1;Parent=MsaT014586.1
Chr3 CDS 75558914 75559399 75558914 ID=cds.MsaT014586.1;Parent=MsaT014586.1
Gene Sequence

>MsaT014586.1

ATGAGAAAGAAGGTGAAACTTGCATTCATGGTCAGTGATTCTGCAAGGAAGATAACATACAACAAAAGAAAAAAGAGTTTGATTAAGAAGGTTAATGAACTCACCACCCTATGTGGGATTGATGGATGCGCTATAGTTTATAGCGAATTTCATTCAGAGCCTGAGGTTTGGCCTTCTCCATGGGAGGTCCAAAGGGTTCTCACAAAGTTAAAAAATTATTCAGAATTCGAGCTAGGAAAGAAGAAGCTGAACCAAGAGAGCTATTTGAGGGAGAGGATTATGAAGTCCAAAGAGCAATTGATGAAATTGGAGAAGAACAATTGGGAGACAGAGAAGTCCTTGATCTTGTTTCAATGTCTTGTCAAAGAAAATTTTGTTGACACTTTGAACACAAATGTTTTGAATGATCTCGCATGGGAAATTAATGAGAAGATGAAGGAGATTACTTCAAAGGTCAATGAACTAGACACAAGTGCAACCAATTAG
Protein sequence

>MsaT014586.1

MRKKVKLAFMVSDSARKITYNKRKKSLIKKVNELTTLCGIDGCAIVYSEFHSEPEVWPSPWEVQRVLTKLKNYSEFELGKKKLNQESYLRERIMKSKEQLMKLEKNNWETEKSLILFQCLVKENFVDTLNTNVLNDLAWEINEKMKEITSKVNELDTSATN*