AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014587


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014587 MtrunA17_Chr3g0107341 96.894 161 5 0 1 161 1 161 1.36e-111 313
MsaG014587 MtrunA17_Chr3g0107351 93.168 161 11 0 1 161 1 161 4.42e-109 307
MsaG014587 MtrunA17_Chr3g0107361 90.683 161 15 0 1 161 1 161 3.45e-105 297
MsaG014587 MtrunA17_Chr3g0107331 78.125 160 35 0 1 160 1 160 8.13e-90 258
MsaG014587 MtrunA17_Chr3g0110131 49.045 157 80 0 2 158 3 159 9.87e-46 149
MsaG014587 MtrunA17_Chr8g0351751 43.750 160 87 2 2 158 3 162 5.65e-43 142
MsaG014587 MtrunA17_Chr3g0107381 41.830 153 89 0 2 154 3 155 1.79e-41 138
MsaG014587 MtrunA17_Chr4g0014321 40.881 159 91 2 2 158 3 160 2.92e-40 135
MsaG014587 MtrunA17_Chr4g0014031 44.025 159 88 1 2 159 3 161 1.26e-39 133
MsaG014587 MtrunA17_Chr1g0197261 43.871 155 86 1 2 156 3 156 1.36e-39 131
MsaG014587 MtrunA17_Chr4g0013041 41.333 150 85 2 4 152 5 152 8.14e-38 128
MsaG014587 MtrunA17_Chr1g0197461 42.038 157 90 1 1 157 1 156 9.44e-38 126
MsaG014587 MtrunA17_Chr5g0432691 43.038 158 90 0 4 161 5 162 1.92e-36 123
MsaG014587 MtrunA17_Chr2g0296831 47.899 119 62 0 2 120 3 121 1.41e-35 120
MsaG014587 MtrunA17_Chr4g0014131 44.654 159 87 1 2 159 3 161 1.54e-35 123
MsaG014587 MtrunA17_Chr4g0014121 45.752 153 82 1 1 152 30 182 1.96e-35 121
MsaG014587 MtrunA17_Chr5g0420351 41.558 154 89 1 2 155 3 155 1.70e-33 115
MsaG014587 MtrunA17_Chr1g0197321 41.667 156 89 2 2 156 3 157 5.07e-33 114
MsaG014587 MtrunA17_Chr4g0012761 42.857 154 87 1 2 155 3 155 3.39e-30 107
MsaG014587 MtrunA17_Chr7g0218151 42.857 154 87 1 2 155 3 155 3.90e-30 107
MsaG014587 MtrunA17_Chr1g0188681 40.132 152 90 1 1 152 2 152 4.73e-30 107
MsaG014587 MtrunA17_Chr1g0188661 42.857 154 87 1 2 155 3 155 3.58e-29 105
MsaG014587 MtrunA17_Chr2g0283941 38.000 150 92 1 4 152 5 154 4.56e-29 107
MsaG014587 MtrunA17_Chr3g0106501 42.308 130 72 2 29 156 2 130 3.55e-28 102
MsaG014587 MtrunA17_Chr1g0193311 40.909 154 90 1 2 155 3 155 4.10e-28 102
MsaG014587 MtrunA17_Chr3g0107461 35.484 155 99 1 2 155 3 157 4.88e-27 99.4
MsaG014587 MtrunA17_Chr1g0188651 39.216 153 88 3 1 152 2 150 8.49e-23 88.6
MsaG014587 MtrunA17_Chr3g0106421 35.385 130 83 1 29 157 2 131 9.38e-23 89.7
MsaG014587 MtrunA17_Chr2g0296861 36.486 148 94 0 5 152 6 153 2.52e-22 87.4
MsaG014587 MtrunA17_Chr7g0267601 31.373 153 104 1 2 154 6 157 4.58e-19 83.2
MsaG014587 MtrunA17_Chr4g0008701 30.065 153 106 1 2 154 6 157 2.19e-18 81.3
MsaG014587 MtrunA17_Chr3g0105791 33.594 128 84 1 29 155 2 129 1.27e-17 74.7
MsaG014587 MtrunA17_Chr3g0128721 28.387 155 111 0 2 156 6 160 3.75e-17 77.4
MsaG014587 MtrunA17_Chr4g0031421 32.812 128 85 1 29 155 2 129 2.43e-16 71.2
MsaG014587 MtrunA17_Chr6g0457851 31.677 161 94 7 2 155 13 164 4.37e-14 68.6
MsaG014587 MtrunA17_Chr6g0449861 33.043 115 70 3 2 110 11 124 2.76e-12 63.2
MsaG014587 MtrunA17_Chr1g0212151 33.621 116 68 4 2 110 3 116 4.47e-12 62.8
MsaG014587 MtrunA17_Chr6g0449851 32.759 116 71 3 2 111 3 117 8.85e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014587 AT5G48670.1 45.033 151 83 0 2 152 3 153 2.97e-36 127
MsaG014587 AT1G65300.1 36.842 152 96 0 1 152 1 152 3.74e-31 113
MsaG014587 AT1G65330.1 37.342 158 99 0 1 158 1 158 3.84e-30 110
MsaG014587 AT5G26630.1 36.306 157 100 0 2 158 3 159 5.52e-28 103
MsaG014587 AT5G27960.1 39.316 117 71 0 4 120 3 119 1.94e-26 102
MsaG014587 AT5G26650.1 33.548 155 103 0 4 158 3 157 2.41e-26 102
MsaG014587 AT3G05860.2 35.948 153 98 0 2 154 3 155 2.50e-25 96.7
MsaG014587 AT3G05860.3 35.948 153 98 0 2 154 3 155 6.70e-25 96.7
MsaG014587 AT3G05860.1 35.948 153 98 0 2 154 3 155 9.20e-25 96.7
MsaG014587 AT5G27810.1 44.565 92 51 0 29 120 2 93 1.56e-23 89.7
MsaG014587 AT1G31630.1 29.814 161 112 1 1 161 1 160 2.38e-22 91.7
MsaG014587 AT1G31640.1 30.464 151 104 1 1 151 1 150 2.46e-20 87.0
MsaG014587 AT5G26580.1 32.886 149 86 1 4 152 5 139 7.95e-20 84.7
MsaG014587 AT2G28700.1 31.579 152 104 0 2 153 3 154 1.89e-18 80.9
MsaG014587 AT1G22590.2 34.906 106 68 1 2 107 3 107 2.25e-16 72.4
MsaG014587 AT2G40210.1 37.374 99 62 0 2 100 3 101 7.66e-13 65.5
MsaG014587 AT5G58890.1 39.130 92 48 3 5 89 6 96 9.01e-11 59.3

Find 33 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAAGTCAAAAGAGAAAT+TGG 0.291455 3:-75577943 MsaT014587.1:CDS
AAAAGAGAAATTGGTGAAAG+TGG 0.384195 3:-75577934 MsaT014587.1:CDS
GAAGGTGAAACTTGCATTCA+TGG 0.384908 3:-75578213 MsaT014587.1:CDS
GAACCAAGAGAGCTATTTGA+TGG 0.414546 3:-75577973 MsaT014587.1:CDS
CTTGTCGGAGACATTCAAAC+AGG 0.421968 3:+75577877 None:intergenic
GGACTTCTCCATCTCCCAAT+TGG 0.425670 3:+75577905 None:intergenic
TTCATTCAGAGCCTGAGGTC+TGG 0.433093 3:-75578073 MsaT014587.1:CDS
AACGGGGAAGGCCAGACCTC+AGG 0.443199 3:+75578062 None:intergenic
TCAAAAGTTATTCAGAAGTC+GGG 0.444131 3:-75578013 MsaT014587.1:CDS
TCTCCATCAAATAGCTCTCT+TGG 0.453856 3:+75577970 None:intergenic
TTCAAAAGTTATTCAGAAGT+CGG 0.458207 3:-75578014 MsaT014587.1:CDS
TAATCCTTTGAACCTCCAAC+GGG 0.460280 3:+75578045 None:intergenic
CTTCCCCGTTGGAGGTTCAA+AGG 0.471467 3:-75578049 MsaT014587.1:CDS
GGTCTGGCCTTCCCCGTTGG+AGG 0.473960 3:-75578057 MsaT014587.1:CDS
TGAGGTCTGGCCTTCCCCGT+TGG 0.479692 3:-75578060 MsaT014587.1:CDS
TGAAAGTGGAGAAAACCAAT+TGG 0.503387 3:-75577920 MsaT014587.1:CDS
ATTCAGAAGTCGGGCAAGGA+AGG 0.517455 3:-75578004 MsaT014587.1:CDS
GATGAACTCACTACCCTATG+TGG 0.541478 3:-75578128 MsaT014587.1:CDS
GAAAGTGGAGAAAACCAATT+GGG 0.551602 3:-75577919 MsaT014587.1:CDS
GCATACATCAATCCCACATA+GGG 0.556455 3:+75578115 None:intergenic
GAGACATTCAAACAGGATCA+AGG 0.560270 3:+75577884 None:intergenic
AAGAGAGCTATTTGATGGAG+AGG 0.560879 3:-75577968 MsaT014587.1:CDS
ATAATCCTTTGAACCTCCAA+CGG 0.563355 3:+75578044 None:intergenic
CTTTGAACCTCCAACGGGGA+AGG 0.577453 3:+75578050 None:intergenic
CGCATACATCAATCCCACAT+AGG 0.579304 3:+75578114 None:intergenic
TGAAATTAATGAGAAGCTGA+AGG 0.586053 3:-75577805 MsaT014587.1:CDS
GGAGAAAACCAATTGGGAGA+TGG 0.587398 3:-75577913 MsaT014587.1:CDS
ATGAACTCACTACCCTATGT+GGG 0.601587 3:-75578127 MsaT014587.1:CDS
AGTTATTCAGAAGTCGGGCA+AGG 0.603949 3:-75578008 MsaT014587.1:CDS
CGAATTTCATTCAGAGCCTG+AGG 0.605230 3:-75578078 MsaT014587.1:CDS
TCATGGTCAATGATTCTGCA+AGG 0.642899 3:-75578196 MsaT014587.1:CDS
TACATTGAGGATGAGAACGA+AGG 0.687755 3:-75578231 None:intergenic
AATCCTTTGAACCTCCAACG+GGG 0.766026 3:+75578046 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 75577758 75578243 75577758 ID=MsaG014587
Chr3 mRNA 75577758 75578243 75577758 ID=MsaT014587.1;Parent=MsaG014587
Chr3 exon 75577758 75578243 75577758 ID=MsaT014587.1.exon1;Parent=MsaT014587.1
Chr3 CDS 75577758 75578243 75577758 ID=cds.MsaT014587.1;Parent=MsaT014587.1
Gene Sequence

>MsaT014587.1

ATGAGAACGAAGGTGAAACTTGCATTCATGGTCAATGATTCTGCAAGGAAAATAACTTACAACAAAAGAAAAAAGAGTCTGAAAAAGATGGTTGATGAACTCACTACCCTATGTGGGATTGATGTATGCGCTATACTGTATAGCGAATTTCATTCAGAGCCTGAGGTCTGGCCTTCCCCGTTGGAGGTTCAAAGGATTATCACAAAGTTCAAAAGTTATTCAGAAGTCGGGCAAGGAAGGAAGAAGTTGAACCAAGAGAGCTATTTGATGGAGAGGATTGTGAAGTCAAAAGAGAAATTGGTGAAAGTGGAGAAAACCAATTGGGAGATGGAGAAGTCCTTGATCCTGTTTGAATGTCTCCGACAAGAAAATTTTATCAACACTTTGAACACAAACGTTTTGAATGATCTAGCATATGAAATTAATGAGAAGCTGAAGGAAATTACTTCGAAGATGAATCAGCTTGACACAAATGAAAGCACTTAG

Protein sequence

>MsaT014587.1

MRTKVKLAFMVNDSARKITYNKRKKSLKKMVDELTTLCGIDVCAILYSEFHSEPEVWPSPLEVQRIITKFKSYSEVGQGRKKLNQESYLMERIVKSKEKLVKVEKTNWEMEKSLILFECLRQENFINTLNTNVLNDLAYEINEKLKEITSKMNQLDTNEST*