AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014588


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014588 MtrunA17_Chr3g0107341 96.273 161 6 0 1 161 1 161 1.39e-111 313
MsaG014588 MtrunA17_Chr3g0107351 91.925 161 13 0 1 161 1 161 1.77e-106 301
MsaG014588 MtrunA17_Chr3g0107361 89.441 161 17 0 1 161 1 161 1.61e-103 293
MsaG014588 MtrunA17_Chr3g0107331 76.875 160 37 0 1 160 1 160 2.02e-87 252
MsaG014588 MtrunA17_Chr3g0110131 48.408 157 81 0 2 158 3 159 1.14e-44 147
MsaG014588 MtrunA17_Chr8g0351751 43.125 160 88 2 2 158 3 162 5.18e-42 140
MsaG014588 MtrunA17_Chr3g0107381 41.176 153 90 0 2 154 3 155 2.68e-40 135
MsaG014588 MtrunA17_Chr4g0014321 40.252 159 92 2 2 158 3 160 1.46e-39 133
MsaG014588 MtrunA17_Chr1g0197261 43.226 155 87 1 2 156 3 156 3.94e-39 130
MsaG014588 MtrunA17_Chr4g0014031 43.396 159 89 1 2 159 3 161 1.33e-38 131
MsaG014588 MtrunA17_Chr4g0013041 40.667 150 86 2 4 152 5 152 7.57e-37 125
MsaG014588 MtrunA17_Chr1g0197461 41.401 157 91 1 1 157 1 156 7.95e-37 124
MsaG014588 MtrunA17_Chr2g0296831 47.059 119 63 0 2 120 3 121 1.78e-35 119
MsaG014588 MtrunA17_Chr5g0432691 41.772 158 92 0 4 161 5 162 5.61e-35 119
MsaG014588 MtrunA17_Chr4g0014121 45.098 153 83 1 1 152 30 182 1.16e-34 119
MsaG014588 MtrunA17_Chr4g0014131 44.025 159 88 1 2 159 3 161 1.72e-34 120
MsaG014588 MtrunA17_Chr1g0197321 42.308 156 88 2 2 156 3 157 1.29e-33 116
MsaG014588 MtrunA17_Chr5g0420351 40.909 154 90 1 2 155 3 155 8.21e-33 114
MsaG014588 MtrunA17_Chr1g0188681 40.132 152 90 1 1 152 2 152 5.16e-30 107
MsaG014588 MtrunA17_Chr4g0012761 42.208 154 88 1 2 155 3 155 7.96e-30 106
MsaG014588 MtrunA17_Chr7g0218151 42.208 154 88 1 2 155 3 155 8.40e-30 106
MsaG014588 MtrunA17_Chr2g0283941 38.000 150 92 1 4 152 5 154 3.24e-29 107
MsaG014588 MtrunA17_Chr1g0188661 42.208 154 88 1 2 155 3 155 1.83e-28 103
MsaG014588 MtrunA17_Chr3g0106501 42.308 130 72 2 29 156 2 130 8.33e-28 101
MsaG014588 MtrunA17_Chr1g0193311 40.260 154 91 1 2 155 3 155 8.65e-28 101
MsaG014588 MtrunA17_Chr3g0107461 34.194 155 101 1 2 155 3 157 3.69e-25 94.7
MsaG014588 MtrunA17_Chr2g0296861 36.486 148 94 0 5 152 6 153 1.99e-22 87.4
MsaG014588 MtrunA17_Chr1g0188651 38.562 153 89 3 1 152 2 150 3.82e-22 86.7
MsaG014588 MtrunA17_Chr3g0106421 34.615 130 84 1 29 157 2 131 6.82e-22 87.8
MsaG014588 MtrunA17_Chr7g0267601 31.373 153 104 1 2 154 6 157 2.09e-19 84.0
MsaG014588 MtrunA17_Chr4g0008701 30.065 153 106 1 2 154 6 157 7.57e-19 82.4
MsaG014588 MtrunA17_Chr3g0128721 28.387 155 111 0 2 156 6 160 2.76e-17 77.8
MsaG014588 MtrunA17_Chr3g0105791 32.812 128 85 1 29 155 2 129 4.33e-17 73.2
MsaG014588 MtrunA17_Chr4g0031421 32.031 128 86 1 29 155 2 129 3.97e-16 70.9
MsaG014588 MtrunA17_Chr6g0457851 31.677 161 94 7 2 155 13 164 1.99e-14 69.3
MsaG014588 MtrunA17_Chr1g0212151 33.621 116 68 4 2 110 3 116 1.92e-11 60.8
MsaG014588 MtrunA17_Chr6g0449861 32.174 115 71 3 2 110 11 124 2.03e-11 60.8
MsaG014588 MtrunA17_Chr6g0449851 36.441 118 64 5 2 111 3 117 4.36e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014588 AT5G48670.1 43.709 151 85 0 2 152 3 153 1.65e-34 123
MsaG014588 AT1G65300.1 36.184 152 97 0 1 152 1 152 1.48e-30 112
MsaG014588 AT1G65330.1 36.709 158 100 0 1 158 1 158 1.56e-29 109
MsaG014588 AT5G26630.1 35.032 157 102 0 2 158 3 159 3.43e-26 99.4
MsaG014588 AT5G27960.1 32.903 155 104 0 4 158 3 157 3.87e-26 101
MsaG014588 AT5G26650.1 32.903 155 104 0 4 158 3 157 3.95e-26 102
MsaG014588 AT3G05860.2 35.294 153 99 0 2 154 3 155 8.01e-25 95.5
MsaG014588 AT3G05860.3 35.294 153 99 0 2 154 3 155 1.65e-24 95.5
MsaG014588 AT3G05860.1 35.294 153 99 0 2 154 3 155 2.40e-24 95.5
MsaG014588 AT5G27810.1 43.478 92 52 0 29 120 2 93 3.02e-23 89.0
MsaG014588 AT1G31630.1 29.814 161 112 1 1 161 1 160 9.33e-23 92.4
MsaG014588 AT1G31640.1 30.464 151 104 1 1 151 1 150 1.55e-20 87.4
MsaG014588 AT5G26580.1 32.215 149 87 1 4 152 5 139 1.70e-19 84.0
MsaG014588 AT2G28700.1 30.921 152 105 0 2 153 3 154 6.50e-18 79.3
MsaG014588 AT1G22590.2 34.906 106 68 1 2 107 3 107 9.75e-17 73.2
MsaG014588 AT2G40210.1 36.364 99 63 0 2 100 3 101 1.05e-12 65.1
MsaG014588 AT5G58890.1 33.125 160 78 9 5 151 6 149 9.64e-12 62.0

Find 34 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAAGTCAAAAGAGAAAT+TGG 0.291455 3:-75602795 MsaT014588.1:CDS
AAAAGAGAAATTGGTGAAAG+TGG 0.384195 3:-75602786 MsaT014588.1:CDS
GAAGGTGAAACTTGCATTCA+TGG 0.384908 3:-75603065 MsaT014588.1:CDS
GAACCAAGAGAGCTATTTGA+TGG 0.414546 3:-75602825 MsaT014588.1:CDS
CTTGTCGGAGACATTCAAAC+AGG 0.421968 3:+75602729 None:intergenic
GGACTTCTCCATCTCCCAAT+TGG 0.425670 3:+75602757 None:intergenic
AGAGGTCTGGCCTTCCCCAT+TGG 0.437778 3:-75602912 MsaT014588.1:CDS
TCAAAAGTTATTCAGAAGTC+GGG 0.444131 3:-75602865 MsaT014588.1:CDS
TCTCCATCAAATAGCTCTCT+TGG 0.453856 3:+75602822 None:intergenic
TTCAAAAGTTATTCAGAAGT+CGG 0.458207 3:-75602866 MsaT014588.1:CDS
CTTCCCCATTGGAGGTTCAA+AGG 0.464729 3:-75602901 MsaT014588.1:CDS
GGTCTGGCCTTCCCCATTGG+AGG 0.467393 3:-75602909 MsaT014588.1:CDS
TTCAATCAGAGCCAGAGGTC+TGG 0.467622 3:-75602925 MsaT014588.1:CDS
AATGGGGAAGGCCAGACCTC+TGG 0.470946 3:+75602914 None:intergenic
TAATCCTTTGAACCTCCAAT+GGG 0.502458 3:+75602897 None:intergenic
TGAAAGTGGAGAAAACCAAT+TGG 0.503387 3:-75602772 MsaT014588.1:CDS
ATTCAGAAGTCGGGCAAGGA+AGG 0.517455 3:-75602856 MsaT014588.1:CDS
ATAATCCTTTGAACCTCCAA+TGG 0.537452 3:+75602896 None:intergenic
GATGAACTCACTACCCTATG+TGG 0.541478 3:-75602980 MsaT014588.1:CDS
GAAAGTGGAGAAAACCAATT+GGG 0.551602 3:-75602771 MsaT014588.1:CDS
GAGACATTCAAACAGGATCA+AGG 0.560270 3:+75602736 None:intergenic
AAGAGAGCTATTTGATGGAG+AGG 0.560879 3:-75602820 MsaT014588.1:CDS
CAGAAGTCGGGCAAGGAAGG+AGG 0.561788 3:-75602853 MsaT014588.1:CDS
GCACACATCAATCCCACATA+GGG 0.576358 3:+75602967 None:intergenic
TGAAATTAATGAGAAGCTGA+AGG 0.586463 3:-75602657 MsaT014588.1:CDS
GGAGAAAACCAATTGGGAGA+TGG 0.587398 3:-75602765 MsaT014588.1:CDS
ATGAACTCACTACCCTATGT+GGG 0.601587 3:-75602979 MsaT014588.1:CDS
AGTTATTCAGAAGTCGGGCA+AGG 0.603949 3:-75602860 MsaT014588.1:CDS
CGCACACATCAATCCCACAT+AGG 0.604773 3:+75602966 None:intergenic
CTTTGAACCTCCAATGGGGA+AGG 0.605253 3:+75602902 None:intergenic
TCATGGTCAATGATTCTGCA+AGG 0.642899 3:-75603048 MsaT014588.1:CDS
CGAATTTCAATCAGAGCCAG+AGG 0.648177 3:-75602930 MsaT014588.1:CDS
TACATTGAGGATGAGAACGA+AGG 0.657193 3:-75603083 None:intergenic
AATCCTTTGAACCTCCAATG+GGG 0.726583 3:+75602898 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 75602610 75603095 75602610 ID=MsaG014588
Chr3 mRNA 75602610 75603095 75602610 ID=MsaT014588.1;Parent=MsaG014588
Chr3 exon 75602610 75603095 75602610 ID=MsaT014588.1.exon1;Parent=MsaT014588.1
Chr3 CDS 75602610 75603095 75602610 ID=cds.MsaT014588.1;Parent=MsaT014588.1
Gene Sequence

>MsaT014588.1

ATGAGAACGAAGGTGAAACTTGCATTCATGGTCAATGATTCTGCAAGGAAAATAACTTACAACAAAAGAAAAAAGAGTCTGAAAAAGATGGTTGATGAACTCACTACCCTATGTGGGATTGATGTGTGCGCTATACTTTATAGCGAATTTCAATCAGAGCCAGAGGTCTGGCCTTCCCCATTGGAGGTTCAAAGGATTATCACAAAGTTCAAAAGTTATTCAGAAGTCGGGCAAGGAAGGAGGAAGTTGAACCAAGAGAGCTATTTGATGGAGAGGATTGTGAAGTCAAAAGAGAAATTGGTGAAAGTGGAGAAAACCAATTGGGAGATGGAGAAGTCCTTGATCCTGTTTGAATGTCTCCGACAAGAAAATTTTATCAACACTTTGAACACAAACGTTTTGAATGATCTAGCATGTGAAATTAATGAGAAGCTGAAGGAAATTACTTCAAAGATGAATCAGCTTGACACAAATGAAAGCACTTAG

Protein sequence

>MsaT014588.1

MRTKVKLAFMVNDSARKITYNKRKKSLKKMVDELTTLCGIDVCAILYSEFQSEPEVWPSPLEVQRIITKFKSYSEVGQGRRKLNQESYLMERIVKSKEKLVKVEKTNWEMEKSLILFECLRQENFINTLNTNVLNDLACEINEKLKEITSKMNQLDTNEST*