AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014589

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014589 MtrunA17_Chr3g0107351 91.304 161 14 0 1 161 1 161 6.87e-107 301
MsaG014589 MtrunA17_Chr3g0107341 88.820 161 18 0 1 161 1 161 4.87e-103 292
MsaG014589 MtrunA17_Chr3g0107361 86.957 161 21 0 1 161 1 161 1.15e-100 286
MsaG014589 MtrunA17_Chr3g0107331 78.750 160 34 0 1 160 1 160 2.71e-89 257
MsaG014589 MtrunA17_Chr3g0110131 49.682 157 79 0 2 158 3 159 1.17e-44 147
MsaG014589 MtrunA17_Chr4g0014031 47.799 159 82 1 2 159 3 161 1.52e-42 141
MsaG014589 MtrunA17_Chr8g0351751 46.250 160 83 2 2 158 3 162 2.57e-42 141
MsaG014589 MtrunA17_Chr3g0107381 42.405 158 91 0 2 159 3 160 8.69e-41 136
MsaG014589 MtrunA17_Chr4g0013041 42.484 153 79 3 4 152 5 152 1.79e-38 130
MsaG014589 MtrunA17_Chr4g0014121 49.020 153 77 1 1 152 30 182 2.26e-38 129
MsaG014589 MtrunA17_Chr4g0014321 40.252 159 92 2 2 158 3 160 5.51e-38 129
MsaG014589 MtrunA17_Chr1g0197261 44.516 155 85 1 2 156 3 156 8.19e-38 127
MsaG014589 MtrunA17_Chr4g0014131 46.541 159 84 1 2 159 3 161 5.20e-37 127
MsaG014589 MtrunA17_Chr2g0296831 49.167 120 61 0 2 121 3 122 1.28e-35 120
MsaG014589 MtrunA17_Chr1g0197461 42.038 157 90 1 1 157 1 156 4.39e-35 120
MsaG014589 MtrunA17_Chr5g0432691 41.772 158 92 0 4 161 5 162 2.28e-34 118
MsaG014589 MtrunA17_Chr1g0197321 42.308 156 88 2 2 156 3 157 1.56e-31 110
MsaG014589 MtrunA17_Chr5g0420351 40.909 154 90 1 2 155 3 155 7.63e-31 108
MsaG014589 MtrunA17_Chr2g0283941 41.781 146 84 1 4 148 5 150 7.90e-30 108
MsaG014589 MtrunA17_Chr1g0188681 40.132 152 90 1 1 152 2 152 2.92e-28 102
MsaG014589 MtrunA17_Chr4g0012761 42.857 154 87 1 2 155 3 155 3.25e-28 102
MsaG014589 MtrunA17_Chr3g0107461 34.839 155 100 1 2 155 3 157 1.70e-27 100
MsaG014589 MtrunA17_Chr1g0188661 42.857 154 87 1 2 155 3 155 2.61e-27 100
MsaG014589 MtrunA17_Chr7g0218151 42.208 154 88 1 2 155 3 155 4.07e-27 99.8
MsaG014589 MtrunA17_Chr3g0106501 40.769 130 74 2 29 156 2 130 1.29e-25 95.9
MsaG014589 MtrunA17_Chr1g0193311 40.260 154 91 1 2 155 3 155 2.02e-25 95.1
MsaG014589 MtrunA17_Chr3g0106421 36.923 130 81 1 29 157 2 131 2.56e-23 91.3
MsaG014589 MtrunA17_Chr1g0188651 39.216 153 88 3 1 152 2 150 4.85e-21 84.0
MsaG014589 MtrunA17_Chr2g0296861 35.811 148 95 0 5 152 6 153 1.61e-20 82.8
MsaG014589 MtrunA17_Chr7g0267601 31.373 153 104 1 2 154 6 157 2.81e-17 77.8
MsaG014589 MtrunA17_Chr4g0008701 30.719 153 105 1 2 154 6 157 3.69e-17 77.4
MsaG014589 MtrunA17_Chr3g0105791 32.090 134 78 2 29 155 2 129 3.04e-16 71.2
MsaG014589 MtrunA17_Chr3g0128721 28.387 155 111 0 2 156 6 160 3.48e-16 74.7
MsaG014589 MtrunA17_Chr4g0031421 30.597 134 80 2 29 155 2 129 1.76e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014589 AT5G48670.1 43.396 159 90 0 2 160 3 161 7.37e-34 121
MsaG014589 AT1G65300.1 37.500 152 95 0 1 152 1 152 1.61e-29 109
MsaG014589 AT1G65330.1 37.975 158 98 0 1 158 1 158 1.93e-28 106
MsaG014589 AT5G26630.1 37.580 157 98 0 2 158 3 159 3.56e-27 101
MsaG014589 AT3G05860.2 35.948 153 98 0 2 154 3 155 2.68e-24 94.0
MsaG014589 AT3G05860.3 35.948 153 98 0 2 154 3 155 8.85e-24 93.6
MsaG014589 AT3G05860.1 35.948 153 98 0 2 154 3 155 1.21e-23 93.6
MsaG014589 AT5G27960.1 31.613 155 106 0 4 158 3 157 4.18e-23 93.2
MsaG014589 AT5G26650.1 31.613 155 106 0 4 158 3 157 5.13e-23 93.6
MsaG014589 AT5G27810.1 43.478 92 52 0 29 120 2 93 8.12e-22 85.1
MsaG014589 AT1G31630.1 28.571 161 114 1 1 161 1 160 1.55e-18 81.3
MsaG014589 AT1G31640.1 29.139 151 106 1 1 151 1 150 2.30e-17 78.6
MsaG014589 AT5G26580.1 31.544 149 88 1 4 152 5 139 3.32e-17 77.4
MsaG014589 AT2G28700.1 30.921 152 105 0 2 153 3 154 9.47e-17 76.3
MsaG014589 AT1G22590.2 34.615 104 67 1 2 105 3 105 7.01e-15 68.6
MsaG014589 AT2G40210.1 36.364 99 63 0 2 100 3 101 1.13e-11 62.0

Find 35 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAAGTTATTCAGAATT+CGG 0.234784 3:-75650373 MsaT014589.1:CDS
TCAAAAGTTATTCAGAATTC+GGG 0.321565 3:-75650372 MsaT014589.1:CDS
TGTGAAGTCAAAGGAGAAAT+TGG 0.334788 3:-75650302 MsaT014589.1:CDS
GAAGGTGAAACTTGCATTCA+TGG 0.384908 3:-75650572 MsaT014589.1:CDS
GGAAATTAATGACAAGCTTA+AGG 0.410494 3:-75650164 MsaT014589.1:CDS
TTTGCATCAAATAGCTCTCT+TGG 0.411513 3:+75650329 None:intergenic
AAAGGAGAAATTGGTGAAAG+TGG 0.424135 3:-75650293 MsaT014589.1:CDS
GGACTTCTCCATCTCCCAAT+TGG 0.458797 3:+75650264 None:intergenic
GAAAGTGGAGAAGGCCAATT+GGG 0.464215 3:-75650278 MsaT014589.1:CDS
CTTCCCCATTGGAGGTTCAA+AGG 0.464729 3:-75650408 MsaT014589.1:CDS
TGAAAGTGGAGAAGGCCAAT+TGG 0.469746 3:-75650279 MsaT014589.1:CDS
GGTATGGCCTTCCCCATTGG+AGG 0.482213 3:-75650416 MsaT014589.1:CDS
TAATCCTTTGAACCTCCAAT+GGG 0.502458 3:+75650404 None:intergenic
AATGGGGAAGGCCATACCTC+TGG 0.503415 3:+75650421 None:intergenic
AGAGGTATGGCCTTCCCCAT+TGG 0.513167 3:-75650419 MsaT014589.1:CDS
AAGAGAGCTATTTGATGCAA+AGG 0.518848 3:-75650327 MsaT014589.1:CDS
ATAATCCTTTGAACCTCCAA+TGG 0.537452 3:+75650403 None:intergenic
GATGAACTCACTACCCTATG+TGG 0.541478 3:-75650487 MsaT014589.1:CDS
GCCTACATCAATCCCACATA+GGG 0.544450 3:+75650474 None:intergenic
ATTCAGAATTCGGGCAAGGA+AGG 0.545789 3:-75650363 MsaT014589.1:CDS
TTCATTCAGAGCCAGAGGTA+TGG 0.547696 3:-75650432 MsaT014589.1:CDS
ACCCTATGTGGGATTGATGT+AGG 0.551331 3:-75650475 MsaT014589.1:CDS
AAGACACTGTAACAAGATCA+AGG 0.558997 3:+75650243 None:intergenic
GGAGAAGGCCAATTGGGAGA+TGG 0.573197 3:-75650272 MsaT014589.1:CDS
AGTTATTCAGAATTCGGGCA+AGG 0.591523 3:-75650367 MsaT014589.1:CDS
CGCCTACATCAATCCCACAT+AGG 0.593049 3:+75650473 None:intergenic
GAAATTGGTGAAAGTGGAGA+AGG 0.595364 3:-75650287 MsaT014589.1:CDS
TGGTTATCGAAATCTCTAAG+TGG 0.599795 3:+75650102 None:intergenic
GCAAAGGATTGTGAAGTCAA+AGG 0.600220 3:-75650311 MsaT014589.1:CDS
ATGAACTCACTACCCTATGT+GGG 0.601587 3:-75650486 MsaT014589.1:CDS
CTTTGAACCTCCAATGGGGA+AGG 0.605253 3:+75650409 None:intergenic
CGAATTTCATTCAGAGCCAG+AGG 0.606659 3:-75650437 MsaT014589.1:CDS
TCATGGTCAATGATTCTGCA+AGG 0.642899 3:-75650555 MsaT014589.1:CDS
TACATTGAGGATGAGAACGA+AGG 0.700174 3:-75650590 None:intergenic
AATCCTTTGAACCTCCAATG+GGG 0.726583 3:+75650405 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 75650117 75650602 75650117 ID=MsaG014589
Chr3 mRNA 75650117 75650602 75650117 ID=MsaT014589.1;Parent=MsaG014589
Chr3 exon 75650117 75650602 75650117 ID=MsaT014589.1.exon1;Parent=MsaT014589.1
Chr3 CDS 75650117 75650602 75650117 ID=cds.MsaT014589.1;Parent=MsaT014589.1
Gene Sequence

>MsaT014589.1

ATGAGAACGAAGGTGAAACTTGCATTCATGGTCAATGATTCTGCAAGGAAAATAACTTACAACAAAAGAAAAAAGAGTCTGAAGAAGATGGTTGATGAACTCACTACCCTATGTGGGATTGATGTAGGCGCTATAGTTTATAGCGAATTTCATTCAGAGCCAGAGGTATGGCCTTCCCCATTGGAGGTTCAAAGGATTATCACAAAGTTCAAAAGTTATTCAGAATTCGGGCAAGGAAGGAAGAAGTTGAACCAAGAGAGCTATTTGATGCAAAGGATTGTGAAGTCAAAGGAGAAATTGGTGAAAGTGGAGAAGGCCAATTGGGAGATGGAGAAGTCCTTGATCTTGTTACAGTGTCTTAATCAAGAAAATTATGTCAGCACTTTGAACACAAATGTTTTGAATGATCTAGCATGGGAAATTAATGACAAGCTTAAGGAAATTACTTCAAAGATGAATCAACTTGACACAAATGAAACCACTTAG
Protein sequence

>MsaT014589.1

MRTKVKLAFMVNDSARKITYNKRKKSLKKMVDELTTLCGIDVGAIVYSEFHSEPEVWPSPLEVQRIITKFKSYSEFGQGRKKLNQESYLMQRIVKSKEKLVKVEKANWEMEKSLILLQCLNQENYVSTLNTNVLNDLAWEINDKLKEITSKMNQLDTNETT*