AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014591

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014591 MtrunA17_Chr3g0107461 87.578 161 20 0 1 161 1 161 3.21e-103 292
MsaG014591 MtrunA17_Chr4g0031421 77.444 133 30 0 29 161 1 133 1.60e-71 211
MsaG014591 MtrunA17_Chr3g0105791 75.188 133 33 0 29 161 1 133 5.03e-69 205
MsaG014591 MtrunA17_Chr3g0107381 54.545 154 69 1 1 154 1 153 8.74e-56 174
MsaG014591 MtrunA17_Chr3g0110131 49.032 155 78 1 1 155 1 154 2.20e-41 138
MsaG014591 MtrunA17_Chr2g0283941 42.308 156 90 0 5 160 5 160 2.93e-40 135
MsaG014591 MtrunA17_Chr2g0296861 46.452 155 82 1 1 155 1 154 2.27e-39 130
MsaG014591 MtrunA17_Chr8g0351751 41.509 159 91 1 1 157 1 159 2.39e-36 125
MsaG014591 MtrunA17_Chr4g0014321 37.662 154 96 0 1 154 1 154 3.87e-36 124
MsaG014591 MtrunA17_Chr4g0014131 44.099 161 90 0 1 161 1 161 8.88e-36 124
MsaG014591 MtrunA17_Chr5g0432691 38.961 154 93 1 1 154 1 153 1.43e-35 121
MsaG014591 MtrunA17_Chr4g0014031 43.590 156 88 0 1 156 1 156 2.79e-35 122
MsaG014591 MtrunA17_Chr3g0107331 41.722 151 87 1 3 153 2 151 1.34e-34 119
MsaG014591 MtrunA17_Chr3g0106421 44.697 132 73 0 29 160 1 132 1.00e-33 118
MsaG014591 MtrunA17_Chr4g0014121 40.385 156 93 0 1 156 29 184 1.62e-32 114
MsaG014591 MtrunA17_Chr2g0296831 48.361 122 63 0 1 122 1 122 1.61e-31 109
MsaG014591 MtrunA17_Chr3g0106501 45.736 129 70 0 29 157 1 129 2.89e-31 110
MsaG014591 MtrunA17_Chr3g0107351 37.419 155 96 1 3 157 2 155 1.64e-30 108
MsaG014591 MtrunA17_Chr4g0013041 37.209 172 87 4 1 157 1 166 1.75e-30 109
MsaG014591 MtrunA17_Chr5g0420351 38.217 157 95 2 1 157 1 155 2.48e-30 107
MsaG014591 MtrunA17_Chr3g0107341 38.158 152 93 1 3 154 2 152 1.57e-28 103
MsaG014591 MtrunA17_Chr3g0107361 38.158 152 93 1 3 154 2 152 1.81e-28 103
MsaG014591 MtrunA17_Chr1g0188681 38.217 157 95 2 1 157 1 155 2.71e-28 102
MsaG014591 MtrunA17_Chr1g0197461 34.194 155 100 2 3 157 2 154 5.55e-27 99.4
MsaG014591 MtrunA17_Chr1g0188661 37.342 158 95 3 1 157 1 155 8.53e-26 96.3
MsaG014591 MtrunA17_Chr4g0012761 37.580 157 96 2 1 157 1 155 1.58e-25 95.5
MsaG014591 MtrunA17_Chr1g0197261 32.903 155 100 3 1 154 1 152 2.13e-24 92.4
MsaG014591 MtrunA17_Chr1g0197321 33.333 159 101 3 1 157 1 156 5.83e-24 91.7
MsaG014591 MtrunA17_Chr7g0218151 35.669 157 99 2 1 157 1 155 6.91e-24 91.3
MsaG014591 MtrunA17_Chr1g0188651 36.025 161 99 2 1 161 1 157 1.03e-23 90.9
MsaG014591 MtrunA17_Chr1g0193311 36.076 158 97 3 1 157 1 155 3.62e-23 89.4
MsaG014591 MtrunA17_Chr4g0008701 28.188 149 100 3 3 148 6 150 1.48e-14 70.1
MsaG014591 MtrunA17_Chr7g0267601 26.452 155 106 4 3 154 6 155 5.19e-14 68.6
MsaG014591 MtrunA17_Chr3g0128721 24.183 153 107 2 5 153 8 155 7.83e-12 62.4
MsaG014591 MtrunA17_Chr6g0457851 29.204 113 73 3 1 107 11 122 2.42e-11 60.5
MsaG014591 MtrunA17_Chr1g0212151 28.448 116 76 3 1 110 1 115 4.25e-11 60.1
MsaG014591 MtrunA17_Chr2g0279821 30.973 113 76 1 1 113 1 111 5.91e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014591 AT5G48670.1 48.718 156 77 2 1 155 1 154 3.93e-37 130
MsaG014591 AT3G05860.2 48.718 117 56 1 1 113 1 117 1.30e-30 110
MsaG014591 AT3G05860.3 47.107 121 60 1 1 117 1 121 2.91e-30 110
MsaG014591 AT3G05860.1 47.107 121 60 1 1 117 1 121 3.83e-30 110
MsaG014591 AT1G65330.1 41.216 148 86 1 3 150 2 148 5.17e-30 110
MsaG014591 AT1G65300.1 39.490 157 93 2 5 161 4 158 2.36e-29 108
MsaG014591 AT5G27810.1 51.685 89 43 0 29 117 1 89 3.85e-27 99.0
MsaG014591 AT5G26650.1 35.294 153 98 1 5 157 3 154 1.13e-23 95.5
MsaG014591 AT5G27960.1 37.226 137 85 1 5 141 3 138 1.65e-22 91.7
MsaG014591 AT1G31630.1 35.625 160 85 5 3 154 2 151 1.21e-21 89.7
MsaG014591 AT1G22590.2 32.500 160 91 2 1 157 1 146 1.45e-21 85.9
MsaG014591 AT1G31640.1 38.365 159 84 4 3 155 2 152 2.70e-21 89.7
MsaG014591 AT5G26630.1 34.194 155 101 1 1 155 1 154 8.14e-21 85.1
MsaG014591 AT2G28700.1 32.667 150 100 1 1 150 1 149 8.24e-21 87.0
MsaG014591 AT5G06500.1 33.333 147 88 3 1 147 1 137 1.09e-19 82.8
MsaG014591 AT5G26580.1 36.634 101 50 1 1 101 1 87 2.83e-13 66.6
MsaG014591 AT5G26865.1 43.056 72 40 1 24 94 2 73 1.79e-12 61.2
MsaG014591 AT2G40210.1 32.673 101 68 0 1 101 1 101 2.27e-12 63.9
MsaG014591 AT5G27090.1 39.024 82 49 1 14 94 30 111 1.54e-11 60.1
MsaG014591 AT5G27944.1 43.056 72 40 1 24 94 41 112 2.61e-11 60.1

Find 34 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCTGCTTTC+TGG 0.066665 3:-75687068 None:intergenic
ATTCATACCACAGAATTTAA+TGG 0.196701 3:+75687106 MsaT014591.1:CDS
CAGGTTTGGCCATCAGATTC+AGG 0.232242 3:+75686911 MsaT014591.1:CDS
GTTCAAGAGTTTGCATGAAC+TGG 0.380322 3:+75686955 MsaT014591.1:CDS
GATGAAATTAGCACTCTTTG+TGG 0.382102 3:+75686842 MsaT014591.1:CDS
AATGAAATGATGAATGATAT+TGG 0.442815 3:-75687086 None:intergenic
TGATCCTCACTATCATGGCT+AGG 0.463763 3:+75686538 None:intergenic
ACAATCCTCAAGTACAGGTT+TGG 0.465441 3:+75686897 MsaT014591.1:CDS
TATTCTTGAGGCAAACCATT+GGG 0.481117 3:+75687008 MsaT014591.1:CDS
AGGTTTGGCCATCAGATTCA+GGG 0.491311 3:+75686912 MsaT014591.1:CDS
GGGTTAGAAGTGTGATATCC+AGG 0.506627 3:+75686933 MsaT014591.1:CDS
GATCCTCACTATCATGGCTA+GGG 0.513050 3:+75686539 None:intergenic
AAGGAAGGAAACCAGAAAGC+AGG 0.520416 3:+75687057 MsaT014591.1:CDS
CAAATTCTCAGCAATAAAAG+AGG 0.520681 3:-75687164 None:intergenic
GGAGGATGACATTCAGAAAG+AGG 0.520726 3:+75686601 MsaT014591.1:CDS
CTATTCTTGAGGCAAACCAT+TGG 0.534098 3:+75687007 MsaT014591.1:CDS
TAAATATCCATTAAATTCTG+TGG 0.554522 3:-75687113 None:intergenic
TTGCTCGTAAATTATTGCAC+CGG 0.562136 3:-75686873 None:intergenic
GCAAAACAATCCTCAAGTAC+AGG 0.563776 3:+75686892 MsaT014591.1:CDS
CTGCTCATAGACTTTCCCAA+TGG 0.579151 3:-75687023 None:intergenic
TTCATGCAAACTCTTGAACC+TGG 0.581655 3:-75686951 None:intergenic
ACTCTTTGTGGAATAGAAGC+CGG 0.588115 3:+75686854 MsaT014591.1:CDS
GAGGTCGATCAAAAGATGAA+AGG 0.605953 3:+75687190 MsaT014591.1:CDS
CTTCTAACCCCTGAATCTGA+TGG 0.608216 3:-75686920 None:intergenic
GATGGCCAAACCTGTACTTG+AGG 0.609083 3:-75686902 None:intergenic
ATGAGCAGATGATGAAACAA+AGG 0.611511 3:+75687038 MsaT014591.1:CDS
ACATAACATGTGACTCGAAG+AGG 0.616371 3:+75686580 MsaT014591.1:CDS
GGTTTGGCCATCAGATTCAG+GGG 0.628974 3:+75686913 MsaT014591.1:CDS
TATTGCTGAGAATTTGAGGG+AGG 0.639350 3:+75687171 MsaT014591.1:CDS
GCAGATGATGAAACAAAGGA+AGG 0.642572 3:+75687042 MsaT014591.1:CDS
TAACATGTGACTCGAAGAGG+AGG 0.651743 3:+75686583 MsaT014591.1:CDS
ATCCTCACTATCATGGCTAG+GGG 0.657091 3:+75686540 None:intergenic
TCCTCACTATCATGGCTAGG+GGG 0.683399 3:+75686541 None:intergenic
TGGATCAAGAGCTATTCTTG+AGG 0.708311 3:+75686996 MsaT014591.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 75686552 75687231 75686552 ID=MsaG014591
Chr3 mRNA 75686552 75687231 75686552 ID=MsaT014591.1;Parent=MsaG014591
Chr3 exon 75686552 75686630 75686552 ID=MsaT014591.1.exon1;Parent=MsaT014591.1
Chr3 CDS 75686552 75686630 75686552 ID=cds.MsaT014591.1;Parent=MsaT014591.1
Chr3 exon 75686825 75687231 75686825 ID=MsaT014591.1.exon2;Parent=MsaT014591.1
Chr3 CDS 75686825 75687231 75686825 ID=cds.MsaT014591.1;Parent=MsaT014591.1
Gene Sequence

>MsaT014591.1

ATGGCTAGGGGGAAGGTGAAACTAGCATACATAACATGTGACTCGAAGAGGAGGATGACATTCAGAAAGAGGAAAAATGGAATTATGAAGAAGATTGATGAAATTAGCACTCTTTGTGGAATAGAAGCCGGTGCAATAATTTACGAGCAAAACAATCCTCAAGTACAGGTTTGGCCATCAGATTCAGGGGTTAGAAGTGTGATATCCAGGTTCAAGAGTTTGCATGAACTGGAACAAAGTAAAAAGATGGTGGATCAAGAGCTATTCTTGAGGCAAACCATTGGGAAAGTCTATGAGCAGATGATGAAACAAAGGAAGGAAACCAGAAAGCAGGAAATGACCAATATCATTCATCATTTCATTCATACCACAGAATTTAATGGATATTTAATGAGCAAACATGATCTTAATGATTTGTCCTCTTTTATTGCTGAGAATTTGAGGGAGGTCGATCAAAAGATGAAAGGAATGCTAATCGAAGATTGA
Protein sequence

>MsaT014591.1

MARGKVKLAYITCDSKRRMTFRKRKNGIMKKIDEISTLCGIEAGAIIYEQNNPQVQVWPSDSGVRSVISRFKSLHELEQSKKMVDQELFLRQTIGKVYEQMMKQRKETRKQEMTNIIHHFIHTTEFNGYLMSKHDLNDLSSFIAENLREVDQKMKGMLIED*