AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014594


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014594 MtrunA17_Chr3g0107461 91.304 161 14 0 1 161 1 161 1.27e-108 306
MsaG014594 MtrunA17_Chr4g0031421 79.699 133 27 0 29 161 1 133 1.52e-73 217
MsaG014594 MtrunA17_Chr3g0105791 78.947 133 28 0 29 161 1 133 6.74e-73 215
MsaG014594 MtrunA17_Chr3g0107381 55.484 155 66 2 1 154 1 153 4.75e-56 175
MsaG014594 MtrunA17_Chr3g0110131 50.323 155 76 1 1 155 1 154 1.07e-42 142
MsaG014594 MtrunA17_Chr2g0283941 43.590 156 88 0 5 160 5 160 4.92e-41 137
MsaG014594 MtrunA17_Chr2g0296861 47.742 155 80 1 1 155 1 154 1.35e-40 134
MsaG014594 MtrunA17_Chr5g0432691 40.260 154 91 1 1 154 1 153 2.45e-38 128
MsaG014594 MtrunA17_Chr8g0351751 41.509 159 91 1 1 157 1 159 1.21e-36 126
MsaG014594 MtrunA17_Chr4g0014321 38.312 154 95 0 1 154 1 154 1.58e-36 125
MsaG014594 MtrunA17_Chr3g0107331 41.060 151 88 1 3 153 2 151 4.89e-34 117
MsaG014594 MtrunA17_Chr4g0014031 43.590 156 88 0 1 156 1 156 1.36e-33 118
MsaG014594 MtrunA17_Chr4g0014131 43.478 161 91 0 1 161 1 161 1.58e-33 118
MsaG014594 MtrunA17_Chr3g0106421 44.697 132 73 0 29 160 1 132 1.86e-33 117
MsaG014594 MtrunA17_Chr2g0296831 51.282 117 57 0 1 117 1 117 3.17e-33 114
MsaG014594 MtrunA17_Chr3g0106501 44.961 129 71 0 29 157 1 129 2.13e-31 110
MsaG014594 MtrunA17_Chr4g0014121 39.744 156 94 0 1 156 29 184 6.95e-31 110
MsaG014594 MtrunA17_Chr5g0420351 38.854 157 94 2 1 157 1 155 1.23e-30 108
MsaG014594 MtrunA17_Chr4g0013041 36.364 154 96 1 1 154 1 152 1.61e-30 109
MsaG014594 MtrunA17_Chr3g0107351 36.774 155 97 1 3 157 2 155 5.82e-30 107
MsaG014594 MtrunA17_Chr1g0188681 40.000 160 88 4 1 157 1 155 1.35e-28 103
MsaG014594 MtrunA17_Chr3g0107341 36.774 155 97 1 3 157 2 155 5.51e-28 102
MsaG014594 MtrunA17_Chr3g0107361 36.774 155 97 1 3 157 2 155 6.99e-28 101
MsaG014594 MtrunA17_Chr1g0197461 34.839 155 99 2 3 157 2 154 3.30e-27 99.8
MsaG014594 MtrunA17_Chr4g0012761 38.217 157 95 2 1 157 1 155 1.09e-25 95.9
MsaG014594 MtrunA17_Chr1g0188661 37.975 158 94 3 1 157 1 155 1.12e-25 95.9
MsaG014594 MtrunA17_Chr7g0218151 36.306 157 98 2 1 157 1 155 4.49e-24 92.0
MsaG014594 MtrunA17_Chr1g0197261 32.911 158 102 3 1 157 1 155 6.47e-24 91.3
MsaG014594 MtrunA17_Chr1g0188651 36.646 161 98 2 1 161 1 157 8.97e-24 90.9
MsaG014594 MtrunA17_Chr1g0193311 36.709 158 96 3 1 157 1 155 1.92e-23 90.1
MsaG014594 MtrunA17_Chr1g0197321 33.333 159 101 3 1 157 1 156 2.05e-23 90.1
MsaG014594 MtrunA17_Chr4g0008701 27.703 148 103 1 3 150 6 149 1.32e-14 70.1
MsaG014594 MtrunA17_Chr7g0267601 28.947 114 81 0 5 118 8 121 2.44e-13 66.6
MsaG014594 MtrunA17_Chr3g0128721 25.490 153 105 2 5 153 8 155 3.33e-12 63.2
MsaG014594 MtrunA17_Chr6g0457851 29.204 113 73 3 1 107 11 122 5.45e-12 62.4
MsaG014594 MtrunA17_Chr1g0212151 28.448 116 76 3 1 110 1 115 1.13e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014594 AT5G48670.1 50.000 156 75 2 1 155 1 154 7.52e-39 134
MsaG014594 AT3G05860.2 41.447 152 84 2 1 150 1 149 1.05e-30 110
MsaG014594 AT1G65330.1 41.892 148 85 1 3 150 2 148 1.56e-30 112
MsaG014594 AT3G05860.3 53.535 99 46 0 1 99 1 99 2.25e-30 110
MsaG014594 AT3G05860.1 53.535 99 46 0 1 99 1 99 3.51e-30 110
MsaG014594 AT1G65300.1 40.127 157 92 2 5 161 4 158 1.24e-29 109
MsaG014594 AT5G27810.1 53.933 89 41 0 29 117 1 89 5.07e-29 103
MsaG014594 AT5G26650.1 36.601 153 96 1 5 157 3 154 1.39e-25 100
MsaG014594 AT5G27960.1 35.294 153 98 1 5 157 3 154 3.07e-24 96.3
MsaG014594 AT1G31640.1 39.623 159 82 4 3 155 2 152 3.63e-23 94.7
MsaG014594 AT1G31630.1 36.129 155 91 4 3 154 2 151 3.70e-23 93.6
MsaG014594 AT1G22590.2 33.750 160 89 2 1 157 1 146 4.40e-23 89.7
MsaG014594 AT2G28700.1 32.667 150 100 1 1 150 1 149 3.98e-21 88.2
MsaG014594 AT5G26630.1 33.548 155 102 1 1 155 1 154 7.10e-21 85.5
MsaG014594 AT5G06500.1 36.364 110 66 1 1 110 1 106 1.79e-18 79.7
MsaG014594 AT2G40210.1 34.653 101 66 0 1 101 1 101 2.81e-14 69.3
MsaG014594 AT5G26580.1 37.624 101 49 1 1 101 1 87 1.23e-13 67.4
MsaG014594 AT3G57230.1 29.012 162 100 4 1 161 1 148 3.54e-12 62.8
MsaG014594 AT3G57230.4 29.012 162 100 4 1 161 1 148 3.54e-12 62.8
MsaG014594 AT3G57230.2 29.825 114 76 2 1 113 1 111 8.83e-11 57.4

Find 35 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCTGCTTTC+TGG 0.066665 3:+75703995 None:intergenic
ATTCATACCACAGAATTTAA+TGG 0.196701 3:-75703957 MsaT014594.1:CDS
CAGGTTTGGCCATCAGATTC+AGG 0.224706 3:-75704152 MsaT014594.1:CDS
GTTCAGGAGTTTGCCTGGAC+TGG 0.382826 3:-75704108 MsaT014594.1:CDS
CTGAATCTGATGGCCAAACC+TGG 0.394792 3:+75704153 None:intergenic
GAAGTGTGATATCCAGGTTC+AGG 0.406864 3:-75704124 MsaT014594.1:CDS
GATGAAATTAGCACTCTTTG+CGG 0.413383 3:-75704221 MsaT014594.1:CDS
AATGAAATGATGAATGATAT+TGG 0.442815 3:+75703977 None:intergenic
AGGTTTGGCCATCAGATTCA+GGG 0.444978 3:-75704151 MsaT014594.1:CDS
TCCAGGTTCAGGAGTTTGCC+TGG 0.447666 3:-75704113 MsaT014594.1:CDS
ACAATCCTCAAGCCCAGGTT+TGG 0.451431 3:-75704166 MsaT014594.1:CDS
TATTCTTGAGGCAAAGCATT+GGG 0.452810 3:-75704055 MsaT014594.1:CDS
GAGGACAAATCATTAAGATC+AGG 0.474701 3:+75703918 None:intergenic
GGGTTAGAAGTGTGATATCC+AGG 0.506627 3:-75704130 MsaT014594.1:CDS
CACTATCATGGCTAGAGGTA+AGG 0.508320 3:-75704519 None:intergenic
TCCAGGCAAACTCCTGAACC+TGG 0.517673 3:+75704112 None:intergenic
AAGGAAGGAAACCAGAAAGC+AGG 0.520416 3:-75704006 MsaT014594.1:CDS
CAAATTCTCAGCAATAAAAG+AGG 0.520681 3:+75703899 None:intergenic
TAAATATCCATTAAATTCTG+TGG 0.554522 3:+75703950 None:intergenic
GTATTCTTGAGGCAAAGCAT+TGG 0.555152 3:-75704056 MsaT014594.1:CDS
GATGGCCAAACCTGGGCTTG+AGG 0.558655 3:+75704161 None:intergenic
TTGCTCGTAAATTATTGCAC+CGG 0.562136 3:+75704190 None:intergenic
GCAAAACAATCCTCAAGCCC+AGG 0.573782 3:-75704171 MsaT014594.1:CDS
TGAATCTGATGGCCAAACCT+GGG 0.592740 3:+75704154 None:intergenic
GAGGTCGATCAAAAGATGAA+AGG 0.605953 3:-75703873 MsaT014594.1:CDS
CTTCTAACCCCTGAATCTGA+TGG 0.608216 3:+75704143 None:intergenic
ATGAGCAGTTGATGAAACAA+AGG 0.608842 3:-75704025 MsaT014594.1:CDS
ACTCTTTGCGGAATAGAAGC+CGG 0.612299 3:-75704209 MsaT014594.1:CDS
GGTTTGGCCATCAGATTCAG+GGG 0.621539 3:-75704150 MsaT014594.1:CDS
GCAGTTGATGAAACAAAGGA+AGG 0.627441 3:-75704021 MsaT014594.1:CDS
ACATAACATGTGATTCGAAG+AGG 0.635453 3:-75704484 MsaT014594.1:CDS
TATTGCTGAGAATTTGAGGG+AGG 0.639350 3:-75703892 MsaT014594.1:CDS
TAACATGTGATTCGAAGAGG+AGG 0.648629 3:-75704481 MsaT014594.1:CDS
ATCCTCACTATCATGGCTAG+AGG 0.659018 3:-75704524 None:intergenic
TGGATCAAGAGGTATTCTTG+AGG 0.709854 3:-75704067 MsaT014594.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 75703854 75704534 75703854 ID=MsaG014594
Chr3 mRNA 75703854 75704534 75703854 ID=MsaT014594.1;Parent=MsaG014594
Chr3 exon 75703854 75704260 75703854 ID=MsaT014594.1.exon2;Parent=MsaT014594.1
Chr3 CDS 75703854 75704260 75703854 ID=cds.MsaT014594.1;Parent=MsaT014594.1
Chr3 exon 75704456 75704534 75704456 ID=MsaT014594.1.exon1;Parent=MsaT014594.1
Chr3 CDS 75704456 75704534 75704456 ID=cds.MsaT014594.1;Parent=MsaT014594.1
Gene Sequence

>MsaT014594.1

ATGGCTAGAGGTAAGGTGAAACTAGCATACATAACATGTGATTCGAAGAGGAGGATGACATTCAGAAAGAGAAAAAATGGTATTATGAAGAAGATTGATGAAATTAGCACTCTTTGCGGAATAGAAGCCGGTGCAATAATTTACGAGCAAAACAATCCTCAAGCCCAGGTTTGGCCATCAGATTCAGGGGTTAGAAGTGTGATATCCAGGTTCAGGAGTTTGCCTGGACTGGAACAAAGTAAAAAGATGGTGGATCAAGAGGTATTCTTGAGGCAAAGCATTGGGAAAGTCTATGAGCAGTTGATGAAACAAAGGAAGGAAACCAGAAAGCAGGAAATGACCAATATCATTCATCATTTCATTCATACCACAGAATTTAATGGATATTTAATGAGCAAACCTGATCTTAATGATTTGTCCTCTTTTATTGCTGAGAATTTGAGGGAGGTCGATCAAAAGATGAAAGGAATGTTAATCGAAGATTGA

Protein sequence

>MsaT014594.1

MARGKVKLAYITCDSKRRMTFRKRKNGIMKKIDEISTLCGIEAGAIIYEQNNPQAQVWPSDSGVRSVISRFRSLPGLEQSKKMVDQEVFLRQSIGKVYEQLMKQRKETRKQEMTNIIHHFIHTTEFNGYLMSKPDLNDLSSFIAENLREVDQKMKGMLIED*