AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014595

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014595 MtrunA17_Chr2g0296861 66.456 158 53 0 1 158 1 158 5.21e-63 191
MsaG014595 MtrunA17_Chr3g0107461 51.553 161 77 1 1 160 1 161 5.62e-50 157
MsaG014595 MtrunA17_Chr3g0107381 45.625 160 87 0 1 160 1 160 3.10e-46 150
MsaG014595 MtrunA17_Chr2g0283941 45.181 166 84 3 1 161 1 164 5.59e-39 132
MsaG014595 MtrunA17_Chr5g0432691 35.948 153 98 0 1 153 1 153 2.36e-36 123
MsaG014595 MtrunA17_Chr3g0105791 46.269 134 71 1 29 161 1 134 6.33e-35 119
MsaG014595 MtrunA17_Chr3g0110131 42.857 154 88 0 1 154 1 154 1.33e-34 121
MsaG014595 MtrunA17_Chr4g0031421 44.444 135 74 1 29 162 1 135 4.19e-34 117
MsaG014595 MtrunA17_Chr4g0014321 35.714 154 98 1 1 153 1 154 7.90e-32 114
MsaG014595 MtrunA17_Chr4g0014031 40.645 155 91 1 1 154 1 155 1.90e-30 110
MsaG014595 MtrunA17_Chr3g0107331 37.671 146 91 0 7 152 6 151 3.78e-29 105
MsaG014595 MtrunA17_Chr3g0106421 44.697 132 72 1 29 159 1 132 3.66e-28 103
MsaG014595 MtrunA17_Chr8g0351751 38.217 157 94 2 1 154 1 157 3.86e-28 104
MsaG014595 MtrunA17_Chr1g0197261 34.615 156 101 1 1 156 1 155 4.37e-27 99.4
MsaG014595 MtrunA17_Chr5g0420351 35.256 156 100 1 1 156 1 155 5.37e-27 99.4
MsaG014595 MtrunA17_Chr4g0013041 33.766 154 99 2 1 153 1 152 8.85e-27 100
MsaG014595 MtrunA17_Chr1g0188681 34.641 153 99 1 1 153 1 152 3.05e-25 94.7
MsaG014595 MtrunA17_Chr4g0012761 37.908 153 94 1 1 153 1 152 4.73e-25 94.4
MsaG014595 MtrunA17_Chr1g0197461 32.895 152 101 1 7 158 6 156 4.68e-24 91.7
MsaG014595 MtrunA17_Chr4g0014121 37.419 155 96 1 1 154 29 183 6.52e-24 92.4
MsaG014595 MtrunA17_Chr2g0296831 38.655 119 73 0 1 119 1 119 2.44e-23 89.0
MsaG014595 MtrunA17_Chr7g0218151 35.948 153 97 1 1 153 1 152 4.53e-23 89.4
MsaG014595 MtrunA17_Chr1g0188661 33.987 153 100 1 1 153 1 152 4.78e-23 89.4
MsaG014595 MtrunA17_Chr4g0014131 37.580 157 97 1 7 162 7 163 5.61e-23 90.9
MsaG014595 MtrunA17_Chr3g0107361 35.948 153 86 3 7 153 6 152 6.22e-23 89.0
MsaG014595 MtrunA17_Chr3g0107351 31.973 147 100 0 7 153 6 152 1.09e-22 88.2
MsaG014595 MtrunA17_Chr1g0193311 37.013 154 96 1 1 154 1 153 1.51e-22 87.8
MsaG014595 MtrunA17_Chr1g0197321 33.333 156 100 3 1 154 1 154 3.11e-22 87.0
MsaG014595 MtrunA17_Chr3g0107341 31.469 143 98 0 7 149 6 148 5.37e-20 81.3
MsaG014595 MtrunA17_Chr3g0106501 37.037 135 82 2 29 160 1 135 6.63e-20 80.9
MsaG014595 MtrunA17_Chr1g0188651 33.125 160 104 2 1 160 1 157 7.10e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014595 AT5G48670.1 43.226 155 86 2 1 154 1 154 2.06e-31 115
MsaG014595 AT1G65330.1 40.385 156 91 2 7 161 6 160 1.51e-30 112
MsaG014595 AT3G05860.2 36.913 149 94 0 1 149 1 149 7.24e-29 105
MsaG014595 AT3G05860.3 36.913 149 94 0 1 149 1 149 2.25e-28 105
MsaG014595 AT3G05860.1 36.913 149 94 0 1 149 1 149 2.49e-28 105
MsaG014595 AT1G65300.1 35.948 153 98 0 4 156 3 155 3.01e-25 98.2
MsaG014595 AT1G31640.1 35.948 153 93 2 6 156 5 154 2.97e-22 92.4
MsaG014595 AT5G27810.1 43.820 89 50 0 29 117 1 89 5.74e-22 85.5
MsaG014595 AT5G27960.1 31.613 155 106 0 7 161 5 159 8.62e-19 81.6
MsaG014595 AT5G26650.1 31.613 155 106 0 7 161 5 159 1.24e-18 81.6
MsaG014595 AT1G31630.1 33.094 139 86 2 7 142 6 140 1.52e-18 81.3
MsaG014595 AT2G28700.1 34.951 103 67 0 1 103 1 103 4.38e-18 79.7
MsaG014595 AT1G22590.2 29.878 164 100 3 1 158 1 155 6.68e-18 76.3
MsaG014595 AT5G26630.1 29.814 161 113 0 1 161 1 161 5.40e-17 75.1
MsaG014595 AT5G06500.1 30.000 130 83 2 1 126 1 126 2.26e-15 71.2
MsaG014595 AT2G40210.1 28.058 139 99 1 1 139 1 138 4.84e-13 65.9
MsaG014595 AT5G26580.1 25.478 157 103 1 1 157 1 143 2.81e-11 60.8

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTCATTTCATTCTTCT+TGG 0.187611 3:+75714372 None:intergenic
CTCCATTTCAATCTTCATCT+TGG 0.285395 3:+75714221 None:intergenic
GAAAGGCAACATTTGGAAAA+AGG 0.291917 3:-75714854 MsaT014595.1:CDS
TTTGTTTCTTTAACTTCTCT+AGG 0.340953 3:+75714404 None:intergenic
ATTCATTCATACGGGTGAAT+TGG 0.359950 3:-75714338 MsaT014595.1:CDS
TGCTTATTGTTGAGAACCTT+AGG 0.364399 3:-75714276 MsaT014595.1:CDS
TCCATTTCAATCTTCATCTT+GGG 0.367020 3:+75714222 None:intergenic
TCGAAGAGAAAGGCAACATT+TGG 0.402575 3:-75714861 MsaT014595.1:CDS
TGAACTTGTCCAACACCATA+TGG 0.431567 3:+75714503 None:intergenic
TTGTTTCTTTAACTTCTCTA+GGG 0.453550 3:+75714405 None:intergenic
AATTTGTCAATTCATTCATA+CGG 0.461709 3:-75714347 MsaT014595.1:CDS
AATGAAATCACCACCTTATG+TGG 0.471722 3:-75714598 MsaT014595.1:CDS
ACATACATCAATTCCACATA+AGG 0.491397 3:+75714585 None:intergenic
TTCTTTGTTCGACTTCCCTA+AGG 0.503187 3:+75714260 None:intergenic
ATTTGTCAATTCATTCATAC+GGG 0.508251 3:-75714346 MsaT014595.1:CDS
ATTCATACGGGTGAATTGGA+TGG 0.523018 3:-75714334 MsaT014595.1:CDS
GCTTATTGTTGAGAACCTTA+GGG 0.536620 3:-75714275 MsaT014595.1:CDS
AACTGATGACTCGAAGAGAA+AGG 0.544142 3:-75714871 MsaT014595.1:CDS
AAAATCGATGGTGGATCATG+AGG 0.544806 3:-75714455 MsaT014595.1:CDS
GGTGAATTGGATGGAAATGT+TGG 0.586534 3:-75714325 MsaT014595.1:CDS
AAGTCGAACAAAGAATCGAA+TGG 0.606367 3:-75714252 MsaT014595.1:CDS
AAATCGATGGTGGATCATGA+GGG 0.613825 3:-75714454 MsaT014595.1:CDS
TGTATGTGGTATAATCTATG+AGG 0.618109 3:-75714569 MsaT014595.1:CDS
TACATCAATTCCACATAAGG+TGG 0.624843 3:+75714588 None:intergenic
GGAAAACAACACTCAAGCAA+TGG 0.638025 3:-75714548 MsaT014595.1:CDS
ATATGAACGAAGAAAATCGA+TGG 0.638659 3:-75714467 MsaT014595.1:CDS
TGAACGAAGAAAATCGATGG+TGG 0.640332 3:-75714464 MsaT014595.1:CDS
TTATGTGGAATTGATGTATG+TGG 0.644295 3:-75714583 MsaT014595.1:CDS
TGGATCATGAGGGCTTCTTG+AGG 0.670340 3:-75714444 MsaT014595.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 75714228 75714925 75714228 ID=MsaG014595
Chr3 mRNA 75714228 75714925 75714228 ID=MsaT014595.1;Parent=MsaG014595
Chr3 exon 75714228 75714637 75714228 ID=MsaT014595.1.exon2;Parent=MsaT014595.1
Chr3 CDS 75714228 75714637 75714228 ID=cds.MsaT014595.1;Parent=MsaT014595.1
Chr3 exon 75714847 75714925 75714847 ID=MsaT014595.1.exon1;Parent=MsaT014595.1
Chr3 CDS 75714847 75714925 75714847 ID=cds.MsaT014595.1;Parent=MsaT014595.1
Gene Sequence

>MsaT014595.1

ATGGCTGCTAGAAATTCGAAGATTGAATACATAACTGATGACTCGAAGAGAAAGGCAACATTTGGAAAAAGGAAACAAGGCATAATTAAGAAGATTAATGAAATCACCACCTTATGTGGAATTGATGTATGTGGTATAATCTATGAGGAAAACAACACTCAAGCAATGGTTTGTCCATCCAAAGAAAAAGTCCATATGGTGTTGGACAAGTTCATGAGTTTTCCTGAATATGAACGAAGAAAATCGATGGTGGATCATGAGGGCTTCTTGAGGCAATCCATAGCAAAAGCCCTAGAGAAGTTAAAGAAACAAAAGAACAACTCCAAGAAGAATGAAATGAATGATCTAATTTGTCAATTCATTCATACGGGTGAATTGGATGGAAATGTTGGTGAGAGTGATCTTGAAGATCTGTTGTTGCTTATTGTTGAGAACCTTAGGGAAGTCGAACAAAGAATCGAATGGATTCAAGCCCAAGATGAAGATTGA
Protein sequence

>MsaT014595.1

MAARNSKIEYITDDSKRKATFGKRKQGIIKKINEITTLCGIDVCGIIYEENNTQAMVCPSKEKVHMVLDKFMSFPEYERRKSMVDHEGFLRQSIAKALEKLKKQKNNSKKNEMNDLICQFIHTGELDGNVGESDLEDLLLLIVENLREVEQRIEWIQAQDED*