AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014598

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014598 MtrunA17_Chr3g0107491 94.695 377 12 2 1 369 1 377 0.0 733
MsaG014598 MtrunA17_Chr5g0432701 71.598 338 68 6 32 369 49 358 3.68e-168 473
MsaG014598 MtrunA17_Chr3g0128781 47.093 344 135 11 34 369 7 311 2.88e-87 266
MsaG014598 MtrunA17_Chr2g0329551 50.709 282 98 10 34 289 72 338 4.27e-69 223
MsaG014598 MtrunA17_Chr6g0463571 46.595 279 117 14 32 301 26 281 4.09e-54 181
MsaG014598 MtrunA17_Chr4g0036071 39.423 312 113 5 30 266 147 457 1.10e-52 182
MsaG014598 MtrunA17_Chr1g0156051 40.385 260 96 6 61 261 220 479 4.29e-32 126
MsaG014598 MtrunA17_Chr2g0316551 46.316 95 51 0 167 261 206 300 3.64e-20 90.5
MsaG014598 MtrunA17_Chr1g0202051 34.969 163 92 3 104 266 73 221 2.45e-19 87.0
MsaG014598 MtrunA17_Chr2g0325131 47.312 93 49 0 169 261 191 283 3.19e-19 87.8
MsaG014598 MtrunA17_Chr4g0040891 41.379 116 57 1 169 273 194 309 1.57e-18 85.5
MsaG014598 MtrunA17_Chr2g0306581 63.265 49 18 0 157 205 1 49 8.20e-12 62.0
MsaG014598 MtrunA17_Chr8g0380611 41.935 93 49 1 169 261 234 321 1.04e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014598 AT2G40620.1 47.772 404 135 14 5 369 1 367 1.22e-99 300
MsaG014598 AT1G06850.1 64.929 211 63 3 148 358 127 326 9.68e-85 261
MsaG014598 AT2G31370.5 49.632 272 110 10 34 287 58 320 4.93e-66 214
MsaG014598 AT2G31370.2 49.632 272 110 10 34 287 58 320 9.82e-66 214
MsaG014598 AT2G31370.4 49.632 272 110 10 34 287 58 320 9.82e-66 214
MsaG014598 AT2G31370.1 49.632 272 110 10 34 287 58 320 9.82e-66 214
MsaG014598 AT2G31370.7 49.632 272 110 10 34 287 58 320 9.82e-66 214
MsaG014598 AT2G31370.3 49.632 272 110 10 34 287 58 320 9.82e-66 214
MsaG014598 AT2G31370.6 49.632 272 110 10 34 287 58 320 9.82e-66 214
MsaG014598 AT1G06070.1 51.383 253 107 7 34 277 73 318 3.11e-62 205
MsaG014598 AT4G38900.2 40.643 342 104 13 30 276 165 502 1.11e-49 175
MsaG014598 AT4G38900.3 40.643 342 104 13 30 276 165 502 1.11e-49 175
MsaG014598 AT4G38900.1 39.943 348 104 14 30 276 165 508 4.17e-47 168
MsaG014598 AT1G43700.1 62.687 134 49 1 157 289 182 315 9.31e-45 157
MsaG014598 AT2G21230.4 67.826 115 37 0 152 266 353 467 1.70e-42 155
MsaG014598 AT2G21230.1 67.826 115 37 0 152 266 353 467 1.70e-42 155
MsaG014598 AT1G06850.2 87.500 80 10 0 148 227 127 206 2.91e-41 145
MsaG014598 AT2G21230.3 63.636 121 38 1 152 266 353 473 1.77e-39 147
MsaG014598 AT2G21230.2 68.687 99 31 0 152 250 353 451 3.35e-36 137
MsaG014598 AT2G12900.1 41.346 104 60 1 161 264 109 211 3.25e-20 89.7
MsaG014598 AT2G42380.2 45.745 94 51 0 168 261 185 278 2.01e-19 88.2
MsaG014598 AT3G58120.1 46.237 93 50 0 169 261 202 294 2.09e-19 88.6
MsaG014598 AT2G13150.1 41.584 101 58 1 161 261 142 241 2.20e-17 81.6
MsaG014598 AT2G12940.1 39.423 104 62 1 160 263 140 242 5.75e-17 80.9
MsaG014598 AT2G42380.5 45.238 84 46 0 167 250 184 267 1.63e-16 79.3
MsaG014598 AT2G21235.1 34.028 144 63 2 169 280 359 502 1.84e-16 81.3
MsaG014598 AT3G58120.2 46.835 79 42 0 169 247 202 280 1.68e-15 76.6
MsaG014598 AT2G42380.1 40.426 94 45 1 168 261 185 267 5.13e-13 69.7
MsaG014598 AT1G58110.1 27.491 291 149 10 19 261 45 321 2.44e-12 68.2
MsaG014598 AT1G58110.2 27.491 291 149 10 19 261 45 321 2.44e-12 68.2

Find 125 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCTCTGAATCTTATAATA+TGG 0.191240 3:-75746614 MsaT014598.1:CDS
AACATACAATTTCCCCAATT+TGG 0.232116 3:-75746517 MsaT014598.1:CDS
GGAGGATCCGCCGCCGGTTA+AGG 0.280693 3:+75748927 None:intergenic
CAAAGCTCAGCGAGTTTATC+AGG 0.287151 3:+75748622 None:intergenic
CCGTCTCCCCGACGACATTA+TGG 0.308655 3:-75748968 MsaT014598.1:CDS
CGATCAGCTCGGTCGTTTGC+AGG 0.321109 3:-75746419 MsaT014598.1:CDS
TACGGTAATTGCGCCGGCGC+CGG 0.338646 3:-75748796 MsaT014598.1:CDS
AGCTGAGATTTGGTGAGAAT+TGG 0.342600 3:+75746453 None:intergenic
AGATGGGGAGTGACCAAATT+GGG 0.344045 3:+75746504 None:intergenic
GTTTGTTGCAACTGATAATT+CGG 0.347347 3:+75746475 None:intergenic
TGACCTGGTATAATGTCAAC+TGG 0.348388 3:+75747552 None:intergenic
ACTCGTTCCGGCGCCGGCGC+CGG 0.351806 3:+75748783 None:intergenic
CTATGTCTATGCCTCGTTCT+CGG 0.357586 3:+75748718 None:intergenic
CAGATGGGGAGTGACCAAAT+TGG 0.358510 3:+75746503 None:intergenic
AATTCGAATCAGACCGGTTA+CGG 0.377222 3:-75748814 MsaT014598.1:CDS
CCACAACATTCAGGTCCTTC+AGG 0.383951 3:-75746547 MsaT014598.1:CDS
CCCTCTGAATCTTATAATAT+GGG 0.390160 3:-75746613 MsaT014598.1:CDS
GTCGTTATTCCCACTCGTTC+CGG 0.393983 3:+75748771 None:intergenic
TTTGCAACATAGCTGAGATT+TGG 0.394152 3:+75746443 None:intergenic
ACTTCGACGACGAATATGTT+TGG 0.402900 3:-75748676 MsaT014598.1:CDS
GGGGAGACGGTAGTTGATTT+CGG 0.404834 3:+75748981 None:intergenic
TCCCCTTCCGATCCCTTAAC+CGG 0.405073 3:-75748940 MsaT014598.1:CDS
ATGCACCAGATGCAATTTGC+AGG 0.409287 3:-75746589 MsaT014598.1:CDS
GAGGATCCGCCGCCGGTTAA+GGG 0.411594 3:+75748928 None:intergenic
CGTTGATGAAGGAAGTTGAT+AGG 0.413861 3:-75746666 MsaT014598.1:CDS
CTGATAAACTCGCTGAGCTT+TGG 0.417393 3:-75748621 MsaT014598.1:CDS
AATTGCGCCGGCGCCGGCGC+CGG 0.426206 3:-75748790 MsaT014598.1:CDS
CTCGTCGAGGCTAGCGGTGG+AGG 0.435014 3:+75748909 None:intergenic
ATTCCCACTCGTTCCGGCGC+CGG 0.435287 3:+75748777 None:intergenic
GGCGCCGGCGCCGGAACGAG+TGG 0.435854 3:-75748781 MsaT014598.1:CDS
GGAGCGACGGTGGTGAGAGT+GGG 0.442897 3:+75749008 None:intergenic
TTGAACGCGCAGCAGATTGC+CGG 0.443274 3:+75747654 None:intergenic
TGATAATTCGGCACGTCAGA+TGG 0.444587 3:+75746487 None:intergenic
GCCGGCGCAATTACCGTAAC+CGG 0.446445 3:+75748801 None:intergenic
GAGTTGTAGCCTCTGTTTGA+AGG 0.453582 3:+75747582 None:intergenic
GGGAGCGACGGTGGTGAGAG+TGG 0.456660 3:+75749007 None:intergenic
TTCATCCGCGGCAGCGTCAA+CGG 0.465598 3:-75749051 MsaT014598.1:CDS
GATCAGCTCGGTCGTTTGCA+GGG 0.472645 3:-75746418 MsaT014598.1:CDS
GAAAGGTCCATAATGTCGTC+GGG 0.474927 3:+75748961 None:intergenic
TCTGCAATCCATGGAACAAC+AGG 0.475201 3:-75746920 MsaT014598.1:CDS
TTGATCCAAAACGTGCTAAG+AGG 0.475774 3:-75748594 MsaT014598.1:intron
ACTCTCACTTGCAGAGATTG+AGG 0.477229 3:+75746348 None:intergenic
GTCAACGGCGTCTCCGTCGC+CGG 0.483318 3:-75749036 MsaT014598.1:CDS
TTGACCCTGCAAATTGCATC+TGG 0.487667 3:+75746584 None:intergenic
ATTGGGGAAATTGTATGTTC+TGG 0.495354 3:+75746521 None:intergenic
GACGTTGGAAGAAGGTGCAT+TGG 0.495650 3:+75749074 None:intergenic
CTCTCACTTGCAGAGATTGA+GGG 0.495905 3:+75746349 None:intergenic
GAATATGTTTGGAGAGATCA+TGG 0.496464 3:-75748665 MsaT014598.1:CDS
CCATAATGTCGTCGGGGAGA+CGG 0.497730 3:+75748968 None:intergenic
ACGTTGGAAGAAGGTGCATT+GGG 0.501336 3:+75749075 None:intergenic
GATAGGCTTAAGATTGCTAC+CGG 0.505157 3:-75746649 MsaT014598.1:CDS
GCCGGTTAAGGGATCGGAAG+GGG 0.507894 3:+75748939 None:intergenic
CAAGCTCCGTCTGCAATCCA+TGG 0.511411 3:-75746929 MsaT014598.1:CDS
ACGGTAGTTGATTTCGGAGT+GGG 0.511786 3:+75748987 None:intergenic
AGAGTGGGGTTCGCCGGCGA+CGG 0.512976 3:+75749023 None:intergenic
TTCAAGCTCTTGTATATAGC+GGG 0.514078 3:+75747619 None:intergenic
TACATTGACGTCGAGAAGCT+CGG 0.518334 3:-75748856 MsaT014598.1:CDS
TCCGATCCCTTAACCGGCGG+CGG 0.520683 3:-75748934 MsaT014598.1:CDS
GGTGAAGAAGAGATCGTCTT+CGG 0.522787 3:+75748879 None:intergenic
GATAATTCGGCACGTCAGAT+GGG 0.528159 3:+75746488 None:intergenic
AAGGTGTGCCTGTTGTTCCA+TGG 0.533755 3:+75746912 None:intergenic
AGAGGGGTTCATTGCCTCCC+CGG 0.534741 3:+75746630 None:intergenic
GAGCTTCTCGACGTCAATGT+AGG 0.534935 3:+75748858 None:intergenic
ATCAGCTCGGTCGTTTGCAG+GGG 0.540633 3:-75746417 MsaT014598.1:CDS
TCCGCCGCCGGTTAAGGGAT+CGG 0.540686 3:+75748933 None:intergenic
ACCGCTAGCCTCGACGAGAT+CGG 0.544429 3:-75748904 MsaT014598.1:CDS
TGTGCAGAATACTTGCAAAC+CGG 0.544736 3:-75747673 MsaT014598.1:intron
CGGATGAAGACGTTGGAAGA+AGG 0.546097 3:+75749066 None:intergenic
TCTTAATGATGCGTTGATGA+AGG 0.548957 3:-75746677 MsaT014598.1:CDS
TGCCCAGTTGACATTATACC+AGG 0.552743 3:-75747555 MsaT014598.1:intron
CCCATATTATAAGATTCAGA+GGG 0.552973 3:+75746613 None:intergenic
TCCCATATTATAAGATTCAG+AGG 0.556236 3:+75746612 None:intergenic
TGCGCGTTCAAAAGAGAGAA+AGG 0.557307 3:-75747642 MsaT014598.1:CDS
AATAACGACGGAGATAAGAG+TGG 0.559082 3:-75748757 MsaT014598.1:CDS
TTAAGGGATCGGAAGGGGAA+AGG 0.559188 3:+75748944 None:intergenic
GTGGTGAGAGTGGGGTTCGC+CGG 0.559617 3:+75749017 None:intergenic
AGTTGTAGCCTCTGTTTGAA+GGG 0.560730 3:+75747583 None:intergenic
ACCGGTTACGGTAATTGCGC+CGG 0.565327 3:-75748802 MsaT014598.1:CDS
AGACATAGCTACTCGGTGGA+TGG 0.573309 3:-75748703 MsaT014598.1:CDS
AGCGGTGGAGGATCCGCCGC+CGG 0.574294 3:+75748921 None:intergenic
GCGCCGGCGCCGGAACGAGT+GGG 0.574879 3:-75748780 MsaT014598.1:CDS
CCGCCGGTTAAGGGATCGGA+AGG 0.578667 3:+75748937 None:intergenic
TATACAAGAGCTTGAACGCA+AGG 0.583220 3:-75747612 MsaT014598.1:CDS
GTTGTTCCATGGATTGCAGA+CGG 0.584302 3:+75746923 None:intergenic
TCCGATCTCGTCGAGGCTAG+CGG 0.585549 3:+75748903 None:intergenic
GGAAAGGTCCATAATGTCGT+CGG 0.585789 3:+75748960 None:intergenic
GAGCGACGGTGGTGAGAGTG+GGG 0.593146 3:+75749009 None:intergenic
GGGCTTGACATCAGTAGCAA+AGG 0.594432 3:-75746397 MsaT014598.1:CDS
TCTCACTTGCAGAGATTGAG+GGG 0.600065 3:+75746350 None:intergenic
AAGCTCGGTGGAGTAACAAA+CGG 0.602305 3:-75748841 MsaT014598.1:CDS
ATGTTACGACGCCGAGAACG+AGG 0.610427 3:-75748729 MsaT014598.1:CDS
TTTCGGAGTGGGAGCGACGG+TGG 0.611309 3:+75748998 None:intergenic
CCTTCCGATCCCTTAACCGG+CGG 0.614531 3:-75748937 MsaT014598.1:CDS
GTTCAAGCTCTTGTATATAG+CGG 0.616568 3:+75747618 None:intergenic
CAACAGGCACACCTTCGAGA+TGG 0.617131 3:-75746904 MsaT014598.1:intron
AGACGCCGTTGACGCTGCCG+CGG 0.622769 3:+75749046 None:intergenic
CTCACTTGCAGAGATTGAGG+GGG 0.623339 3:+75746351 None:intergenic
ATAGGCTTAAGATTGCTACC+GGG 0.624945 3:-75746648 MsaT014598.1:CDS
TGCACCAGATGCAATTTGCA+GGG 0.627466 3:-75746588 MsaT014598.1:CDS
GGTTCGAATTCGAATCAGAC+CGG 0.629064 3:-75748820 MsaT014598.1:CDS
TGATTTCGGAGTGGGAGCGA+CGG 0.630845 3:+75748995 None:intergenic
ATAATTCGGCACGTCAGATG+GGG 0.631949 3:+75746489 None:intergenic
GATGGGGAGTGACCAAATTG+GGG 0.632562 3:+75746505 None:intergenic
GATCTCGTCGAGGCTAGCGG+TGG 0.633559 3:+75748906 None:intergenic
AATAGTAGCTACCATCTCGA+AGG 0.635096 3:+75746893 None:intergenic
TCTTCCAACGTCTTCATCCG+CGG 0.635241 3:-75749063 MsaT014598.1:CDS
GACGGTAGTTGATTTCGGAG+TGG 0.642345 3:+75748986 None:intergenic
ATAACGACGGAGATAAGAGT+GGG 0.644922 3:-75748756 MsaT014598.1:CDS
GACCTGGTATAATGTCAACT+GGG 0.646695 3:+75747553 None:intergenic
GCTGCCGCGGATGAAGACGT+TGG 0.650000 3:+75749059 None:intergenic
CGCCGGTTAAGGGATCGGAA+GGG 0.652220 3:+75748938 None:intergenic
TGTATGTTCTGGTGACCTGA+AGG 0.654943 3:+75746532 None:intergenic
TAGGCTTAAGATTGCTACCG+GGG 0.656168 3:-75746647 MsaT014598.1:CDS
CCTGAAGGACCTGAATGTTG+TGG 0.674365 3:+75746547 None:intergenic
GGTTCAGACCCTTCAAACAG+AGG 0.679114 3:-75747591 MsaT014598.1:CDS
CTTCGGATCCGATCTCGTCG+AGG 0.680866 3:+75748896 None:intergenic
GAGGCATAGACATAGCTACT+CGG 0.681656 3:-75748710 MsaT014598.1:CDS
GCTTAAGATTGCTACCGGGG+AGG 0.689095 3:-75746644 MsaT014598.1:CDS
CCATATTATAAGATTCAGAG+GGG 0.694210 3:+75746614 None:intergenic
ATTGACGTCGAGAAGCTCGG+TGG 0.694586 3:-75748853 MsaT014598.1:CDS
AAAGGTCCATAATGTCGTCG+GGG 0.698449 3:+75748962 None:intergenic
AGCTCAGCGAGTTTATCAGG+AGG 0.701452 3:+75748625 None:intergenic
GCATAGACATAGCTACTCGG+TGG 0.704637 3:-75748707 MsaT014598.1:CDS
GGAACGAGTGGGAATAACGA+CGG 0.709896 3:-75748769 MsaT014598.1:CDS
ATGTTGCAAAACGATCAGCT+CGG 0.741818 3:-75746430 MsaT014598.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 75746333 75749118 75746333 ID=MsaG014598
Chr3 mRNA 75746333 75749118 75746333 ID=MsaT014598.1;Parent=MsaG014598
Chr3 exon 75746333 75746700 75746333 ID=MsaT014598.1.exon4;Parent=MsaT014598.1
Chr3 CDS 75746333 75746700 75746333 ID=cds.MsaT014598.1;Parent=MsaT014598.1
Chr3 exon 75746905 75746989 75746905 ID=MsaT014598.1.exon3;Parent=MsaT014598.1
Chr3 CDS 75746905 75746989 75746905 ID=cds.MsaT014598.1;Parent=MsaT014598.1
Chr3 exon 75747556 75747688 75747556 ID=MsaT014598.1.exon2;Parent=MsaT014598.1
Chr3 CDS 75747556 75747688 75747556 ID=cds.MsaT014598.1;Parent=MsaT014598.1
Chr3 exon 75748595 75749118 75748595 ID=MsaT014598.1.exon1;Parent=MsaT014598.1
Chr3 CDS 75748595 75749118 75748595 ID=cds.MsaT014598.1;Parent=MsaT014598.1
Gene Sequence

>MsaT014598.1

ATGGCAACACAAATGCAAGATCCCAATGCACCTTCTTCCAACGTCTTCATCCGCGGCAGCGTCAACGGCGTCTCCGTCGCCGGCGAACCCCACTCTCACCACCGTCGCTCCCACTCCGAAATCAACTACCGTCTCCCCGACGACATTATGGACCTTTCCCCTTCCGATCCCTTAACCGGCGGCGGATCCTCCACCGCTAGCCTCGACGAGATCGGATCCGAAGACGATCTCTTCTTCACCTACATTGACGTCGAGAAGCTCGGTGGAGTAACAAACGGTTCGAATTCGAATCAGACCGGTTACGGTAATTGCGCCGGCGCCGGCGCCGGAACGAGTGGGAATAACGACGGAGATAAGAGTGGGAATGATGTTACGACGCCGAGAACGAGGCATAGACATAGCTACTCGGTGGATGGATTAACTTCGACGACGAATATGTTTGGAGAGATCATGGATGCGAAGAAAGCTATGCCTCCTGATAAACTCGCTGAGCTTTGGACTATTGATCCAAAACGTGCTAAGAGAATACTTGCAAACCGGCAATCTGCTGCGCGTTCAAAAGAGAGAAAGGCCCGCTATATACAAGAGCTTGAACGCAAGGTTCAGACCCTTCAAACAGAGGCTACAACTCTCTCTGCCCAGTTGACATTATACCAGAGGGACACAACTGGTCTGAGTTCTGAAAATACTGAGCTCAAGCTCCGTCTGCAATCCATGGAACAACAGGCACACCTTCGAGATGCTCTTAATGATGCGTTGATGAAGGAAGTTGATAGGCTTAAGATTGCTACCGGGGAGGCAATGAACCCCTCTGAATCTTATAATATGGGAATGCACCAGATGCAATTTGCAGGGTCAAATTTCTTTTCAATGCCACAACATTCAGGTCCTTCAGGTCACCAGAACATACAATTTCCCCAATTTGGTCACTCCCCATCTGACGTGCCGAATTATCAGTTGCAACAAACCAATTCTCACCAAATCTCAGCTATGTTGCAAAACGATCAGCTCGGTCGTTTGCAGGGGCTTGACATCAGTAGCAAAGGATCAACCCTCGTGAAATCCGAAACCCCCTCAATCTCTGCAAGTGAGAGTAGCACTACATTTTGA
Protein sequence

>MsaT014598.1

MATQMQDPNAPSSNVFIRGSVNGVSVAGEPHSHHRRSHSEINYRLPDDIMDLSPSDPLTGGGSSTASLDEIGSEDDLFFTYIDVEKLGGVTNGSNSNQTGYGNCAGAGAGTSGNNDGDKSGNDVTTPRTRHRHSYSVDGLTSTTNMFGEIMDAKKAMPPDKLAELWTIDPKRAKRILANRQSAARSKERKARYIQELERKVQTLQTEATTLSAQLTLYQRDTTGLSSENTELKLRLQSMEQQAHLRDALNDALMKEVDRLKIATGEAMNPSESYNMGMHQMQFAGSNFFSMPQHSGPSGHQNIQFPQFGHSPSDVPNYQLQQTNSHQISAMLQNDQLGRLQGLDISSKGSTLVKSETPSISASESSTTF*