AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014764

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014764 MtrunA17_Chr3g0109791 91.986 287 22 1 1 286 2 288 0.0 512
MsaG014764 MtrunA17_Chr5g0433851 60.000 285 104 5 18 293 4 287 4.08e-97 293
MsaG014764 MtrunA17_Chr6g0481061 45.583 283 135 8 1 269 1 278 9.25e-51 174
MsaG014764 MtrunA17_Chr1g0181771 57.143 98 34 1 45 142 36 125 5.49e-35 129
MsaG014764 MtrunA17_Chr8g0348391 40.984 183 87 3 16 196 24 187 2.07e-33 125
MsaG014764 MtrunA17_Chr6g0469431 53.922 102 39 1 36 137 30 123 4.61e-32 119
MsaG014764 MtrunA17_Chr7g0276421 66.019 103 33 1 37 137 7 109 1.02e-31 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014764 AT5G17300.1 49.813 267 97 4 15 271 4 243 6.00e-58 191
MsaG014764 AT5G37260.1 46.614 251 59 6 33 272 12 198 1.18e-51 172
MsaG014764 AT1G18330.1 52.899 138 54 1 44 181 38 164 3.04e-34 128
MsaG014764 AT3G10113.1 52.518 139 55 1 43 181 52 179 3.49e-34 127
MsaG014764 AT1G18330.2 52.518 139 55 1 43 181 63 190 6.61e-34 127
MsaG014764 AT3G09600.1 55.446 101 37 1 37 137 24 116 2.36e-33 124
MsaG014764 AT3G09600.7 55.446 101 37 1 37 137 24 116 2.36e-33 124
MsaG014764 AT3G09600.2 55.446 101 37 1 37 137 24 116 2.47e-33 124
MsaG014764 AT3G09600.6 55.446 101 37 1 37 137 24 116 2.47e-33 124
MsaG014764 AT3G09600.4 55.446 101 37 1 37 137 24 116 2.67e-33 124
MsaG014764 AT1G01520.2 42.045 176 82 5 20 195 26 181 2.82e-32 120
MsaG014764 AT1G01520.4 42.045 176 82 5 20 195 26 181 2.82e-32 120
MsaG014764 AT1G01520.3 42.045 176 82 5 20 195 26 181 3.93e-32 121
MsaG014764 AT1G01520.1 42.045 176 82 5 20 195 26 181 4.45e-32 121
MsaG014764 AT4G01280.1 53.913 115 43 2 23 137 28 132 1.08e-31 120
MsaG014764 AT4G01280.2 53.913 115 43 2 23 137 28 132 1.10e-31 120
MsaG014764 AT5G02840.4 56.989 93 32 1 45 137 37 121 2.53e-31 118
MsaG014764 AT5G02840.1 56.989 93 32 1 45 137 37 121 2.54e-31 119
MsaG014764 AT5G02840.2 56.989 93 32 1 45 137 37 121 2.54e-31 119
MsaG014764 AT5G02840.3 56.989 93 32 1 45 137 37 121 2.55e-31 119
MsaG014764 AT5G52660.1 54.455 101 38 1 37 137 53 145 3.91e-31 119
MsaG014764 AT5G52660.2 54.455 101 38 1 37 137 53 145 4.03e-31 119
MsaG014764 AT5G02840.5 56.989 93 32 1 45 137 37 121 4.05e-31 118
MsaG014764 AT1G01060.8 57.937 126 50 2 45 167 13 138 1.40e-30 122
MsaG014764 AT1G01060.2 57.937 126 50 2 45 167 13 138 1.40e-30 122
MsaG014764 AT1G01060.3 57.937 126 50 2 45 167 13 138 1.40e-30 122
MsaG014764 AT1G01060.1 57.937 126 50 2 45 167 13 138 1.40e-30 122
MsaG014764 AT1G01060.7 57.937 126 50 2 45 167 13 138 1.40e-30 122
MsaG014764 AT1G01060.4 57.937 126 50 2 45 167 13 138 1.45e-30 121
MsaG014764 AT2G46830.1 57.851 121 45 2 37 155 5 121 2.47e-30 120
MsaG014764 AT5G17300.2 43.979 191 80 4 81 271 5 168 5.10e-23 97.4
MsaG014764 AT3G09600.3 51.562 64 23 1 74 137 5 60 2.64e-16 77.4
MsaG014764 AT3G09600.8 51.562 64 23 1 74 137 5 60 2.64e-16 77.4
MsaG014764 AT3G09600.5 51.562 64 23 1 74 137 5 60 2.85e-16 77.0
MsaG014764 AT3G09600.9 51.562 64 23 1 74 137 5 60 2.85e-16 77.0

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCCGGCCATACAGCTTTA+AGG 0.174526 3:-78064576 None:intergenic
CGCGAATGATTATGCTCTTA+AGG 0.273107 3:+78064374 MsaT014764.1:CDS
CTCTGTTTCGATATAGTATA+TGG 0.282091 3:-78064512 None:intergenic
AGAAATTTCAATCCCGAAAA+AGG 0.302937 3:+78065100 MsaT014764.1:CDS
GGAGGAAAATTGGAACTTCT+TGG 0.327774 3:+78065643 MsaT014764.1:CDS
TAGAAAGGGAGGAGGAAAAT+TGG 0.340113 3:+78065633 MsaT014764.1:CDS
AGGAATTTCAATTGATTCCT+TGG 0.346980 3:-78065009 None:intergenic
AAGTGTAGATAAAACTGATT+TGG 0.347735 3:-78065177 None:intergenic
GCCTCAATTGTTGGAAAAGA+TGG 0.348970 3:-78065584 None:intergenic
TCTTACTCTTAATTGAGATA+TGG 0.349705 3:-78065692 None:intergenic
TCTGAGCATGACTTCGAATC+TGG 0.349813 3:-78064759 None:intergenic
ATAAAACTGATTTGGGAGAT+TGG 0.361687 3:-78065169 None:intergenic
TCTAACATGTATGTCATGAT+TGG 0.376918 3:-78065614 None:intergenic
CTGAGTTGAATATGAGTAAC+AGG 0.381118 3:-78064336 None:intergenic
TCAACTAGCTTGCGAGGATA+AGG 0.387638 3:-78065062 None:intergenic
ATCGGATGAACCAAGGATTT+CGG 0.400444 3:-78065204 None:intergenic
TCATGATGAGAAACCTCTTC+TGG 0.400783 3:+78065349 MsaT014764.1:CDS
CGGGCCTGGCGACGCATTGA+AGG 0.426529 3:+78064593 MsaT014764.1:CDS
GGGTGCATAGGCTTACGTTT+TGG 0.427838 3:-78065041 None:intergenic
AGTGTAGATAAAACTGATTT+GGG 0.427868 3:-78065176 None:intergenic
CTTGAAGCCTTAAAGCTGTA+TGG 0.441266 3:+78064569 MsaT014764.1:CDS
TCCGTTTGGTGTATCTGAAT+CGG 0.446379 3:-78065222 None:intergenic
GGTTTGCATGCCTCAATTGT+TGG 0.453490 3:-78065593 None:intergenic
CAGGTCTGAAAACACTTGCT+TGG 0.457820 3:-78065274 None:intergenic
CAACTAGCTTGCGAGGATAA+GGG 0.462388 3:-78065061 None:intergenic
TTCAAAATGTAGGATCAAGA+TGG 0.466323 3:+78064261 MsaT014764.1:intron
TAAAGCTGTATGGCCGGGCC+TGG 0.467187 3:+78064579 MsaT014764.1:CDS
CATACAGCTTTAAGGCTTCA+AGG 0.472400 3:-78064568 None:intergenic
TGGACACAACTAATCGATAT+CGG 0.474217 3:-78065254 None:intergenic
ATCGGAGATAAACTTCCGTT+TGG 0.476291 3:-78065236 None:intergenic
CCAGCTACATTTCCTACTGC+AGG 0.478856 3:-78064306 None:intergenic
CTTCAATGCGTCGCCAGGCC+CGG 0.484298 3:-78064592 None:intergenic
TCATGACATACATGTTAGAA+AGG 0.487347 3:+78065618 MsaT014764.1:CDS
CATACCTTCAATGCGTCGCC+AGG 0.491595 3:-78064597 None:intergenic
AAGCCTTAAAGCTGTATGGC+CGG 0.499730 3:+78064573 MsaT014764.1:CDS
ACACTTGGTTCCGAAATCCT+TGG 0.507598 3:+78065194 MsaT014764.1:CDS
AGGAAGTTAGTTACCAGAAG+AGG 0.529786 3:-78065362 None:intergenic
GATGGCGCTTTGAATTCAGC+AGG 0.532238 3:-78065293 None:intergenic
AGCCTAGCTTCTTCCTCTGA+TGG 0.534421 3:-78065311 None:intergenic
TATCGAAACAGAGAGAAAGA+TGG 0.552512 3:+78064522 MsaT014764.1:CDS
CGCCATCAGAGGAAGAAGCT+AGG 0.552539 3:+78065309 MsaT014764.1:CDS
TCCTACTGCAGGGCTAACAC+TGG 0.552965 3:-78064295 None:intergenic
AGCCTTAAAGCTGTATGGCC+GGG 0.555613 3:+78064574 MsaT014764.1:CDS
CATGACATACATGTTAGAAA+GGG 0.558691 3:+78065619 MsaT014764.1:CDS
TCCTCGCAAGCTAGTTGAGA+TGG 0.569053 3:+78065067 MsaT014764.1:CDS
TCCGATTCAGATACACCAAA+CGG 0.581293 3:+78065221 MsaT014764.1:CDS
TTGCGAGGATAAGGGTGCAT+AGG 0.596615 3:-78065053 None:intergenic
GACATACATGTTAGAAAGGG+AGG 0.598447 3:+78065622 MsaT014764.1:CDS
TTTCTCGACACAGAATATGT+CGG 0.599822 3:+78064721 MsaT014764.1:intron
CCTGCAGTAGGAAATGTAGC+TGG 0.607570 3:+78064306 MsaT014764.1:CDS
CGCAAGCTAGTTGAGATGGT+CGG 0.633361 3:+78065071 MsaT014764.1:CDS
ATACATGTTAGAAAGGGAGG+AGG 0.634259 3:+78065625 MsaT014764.1:CDS
ACCATCTCAACTAGCTTGCG+AGG 0.635742 3:-78065068 None:intergenic
CAGCTACATTTCCTACTGCA+GGG 0.637750 3:-78064305 None:intergenic
TGAATTCAAAGCGCCATCAG+AGG 0.638951 3:+78065298 MsaT014764.1:CDS
TATCTGAATCGGATGAACCA+AGG 0.663936 3:-78065211 None:intergenic
TCCAGTGTTAGCCCTGCAGT+AGG 0.679374 3:+78064294 MsaT014764.1:CDS
TGCAAGCATTACAAACACCA+AGG 0.703682 3:+78064992 MsaT014764.1:CDS
ATATGTCGGTTCAAAGACTG+CGG 0.730672 3:+78064735 MsaT014764.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 78062435 78065706 78062435 ID=MsaG014764
Chr3 mRNA 78062435 78065706 78062435 ID=MsaT014764.1;Parent=MsaG014764
Chr3 exon 78062435 78062446 78062435 ID=MsaT014764.1.exon1;Parent=MsaT014764.1
Chr3 CDS 78062435 78062446 78062435 ID=cds.MsaT014764.1;Parent=MsaT014764.1
Chr3 exon 78064273 78064395 78064273 ID=MsaT014764.1.exon2;Parent=MsaT014764.1
Chr3 CDS 78064273 78064395 78064273 ID=cds.MsaT014764.1;Parent=MsaT014764.1
Chr3 exon 78064503 78064614 78064503 ID=MsaT014764.1.exon3;Parent=MsaT014764.1
Chr3 CDS 78064503 78064614 78064503 ID=cds.MsaT014764.1;Parent=MsaT014764.1
Chr3 exon 78064734 78064795 78064734 ID=MsaT014764.1.exon4;Parent=MsaT014764.1
Chr3 CDS 78064734 78064795 78064734 ID=cds.MsaT014764.1;Parent=MsaT014764.1
Chr3 exon 78064975 78065370 78064975 ID=MsaT014764.1.exon5;Parent=MsaT014764.1
Chr3 CDS 78064975 78065370 78064975 ID=cds.MsaT014764.1;Parent=MsaT014764.1
Chr3 exon 78065467 78065706 78065467 ID=MsaT014764.1.exon6;Parent=MsaT014764.1
Chr3 CDS 78065467 78065706 78065467 ID=cds.MsaT014764.1;Parent=MsaT014764.1
Gene Sequence

>MsaT014764.1

ATGGCGAATCAAGATCAAGATGGATTAACTTGCTCCAGTGTTAGCCCTGCAGTAGGAAATGTAGCTGGATCGCATCCTGTTACTCATATTCAACTCAGCGATCAGTTATCTTGCGCGAATGATTATGCTCTTAAGGCAAGGAAACCATATACTATATCGAAACAGAGAGAAAGATGGACAGATGAAGAACATAAGAAGTTCCTTGAAGCCTTAAAGCTGTATGGCCGGGCCTGGCGACGCATTGAAGAATATGTCGGTTCAAAGACTGCGGTCCAGATTCGAAGTCATGCTCAGAAGTTTTTTTCTAAGGTTCTTCGCGACACGAGTGCAAGCATTACAAACACCAAGGAATCAATTGAAATTCCTCCTCCTAGACCAAAACGTAAGCCTATGCACCCTTATCCTCGCAAGCTAGTTGAGATGGTCGGTAGTAAAGAAATTTCAATCCCGAAAAAGGCAATGAACTCTAATTCTCTGAAGACATCAGATTTTGATCAAGCAAACCAATCTCCCAAATCAGTTTTATCTACACTTGGTTCCGAAATCCTTGGTTCATCCGATTCAGATACACCAAACGGAAGTTTATCTCCGATATCGATTAGTTGTGTCCAAGCAAGTGTTTTCAGACCTGCTGAATTCAAAGCGCCATCAGAGGAAGAAGCTAGGCTAGATGCTAACTCAGCTCATGATGAGAAACCTCTTCTGAAACTCGAGATTCTCTCTGATGAAAGTGTTTCTGCAAAAGAAAGCATAGCAGAAGAATCGTCTCGTCAAACTCTTAAACTTTTTGGGACGACTCTATTTATAAAAGATTTCTGCAAACCATCTTTTCCAACAATTGAGGCATGCAAACCAATCATGACATACATGTTAGAAAGGGAGGAGGAAAATTGGAACTTCTTGGAAAAAGTACACTATGCAAAACATCAGCCATATCTCAATTAA
Protein sequence

>MsaT014764.1

MANQDQDGLTCSSVSPAVGNVAGSHPVTHIQLSDQLSCANDYALKARKPYTISKQRERWTDEEHKKFLEALKLYGRAWRRIEEYVGSKTAVQIRSHAQKFFSKVLRDTSASITNTKESIEIPPPRPKRKPMHPYPRKLVEMVGSKEISIPKKAMNSNSLKTSDFDQANQSPKSVLSTLGSEILGSSDSDTPNGSLSPISISCVQASVFRPAEFKAPSEEEARLDANSAHDEKPLLKLEILSDESVSAKESIAEESSRQTLKLFGTTLFIKDFCKPSFPTIEACKPIMTYMLEREEENWNFLEKVHYAKHQPYLN*