AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014786


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014786 MtrunA17_Chr3g0110131 96.694 242 5 1 1 239 1 242 6.86e-175 480
MsaG014786 MtrunA17_Chr3g0107381 54.357 241 83 4 1 234 1 221 5.60e-83 247
MsaG014786 MtrunA17_Chr8g0351751 56.735 245 95 6 1 235 1 244 1.03e-79 239
MsaG014786 MtrunA17_Chr4g0014321 50.847 236 107 4 1 235 1 228 5.25e-77 232
MsaG014786 MtrunA17_Chr4g0014031 49.794 243 97 6 1 235 1 226 1.74e-65 202
MsaG014786 MtrunA17_Chr4g0013041 61.039 154 57 2 1 153 1 152 3.65e-61 191
MsaG014786 MtrunA17_Chr4g0014131 50.206 243 98 6 1 235 1 228 2.38e-59 187
MsaG014786 MtrunA17_Chr3g0107331 51.899 158 76 0 3 160 2 159 4.43e-55 174
MsaG014786 MtrunA17_Chr4g0014121 61.290 155 59 1 1 154 29 183 7.39e-54 171
MsaG014786 MtrunA17_Chr2g0296831 68.033 122 39 0 1 122 1 122 4.53e-52 164
MsaG014786 MtrunA17_Chr3g0107351 49.682 157 79 0 3 159 2 158 7.88e-51 163
MsaG014786 MtrunA17_Chr3g0107341 49.682 157 79 0 3 159 2 158 3.49e-49 159
MsaG014786 MtrunA17_Chr3g0107361 49.682 157 79 0 3 159 2 158 5.94e-49 158
MsaG014786 MtrunA17_Chr3g0107461 49.032 155 78 1 1 154 1 155 9.47e-49 157
MsaG014786 MtrunA17_Chr5g0420351 50.000 156 77 1 1 156 1 155 1.23e-46 152
MsaG014786 MtrunA17_Chr5g0432691 42.593 162 93 0 1 162 1 162 4.73e-46 150
MsaG014786 MtrunA17_Chr2g0283941 41.294 201 106 3 1 197 1 193 1.38e-42 144
MsaG014786 MtrunA17_Chr1g0188681 46.497 157 83 1 1 157 1 156 4.82e-42 140
MsaG014786 MtrunA17_Chr7g0218151 50.955 157 76 1 1 157 1 156 1.72e-41 139
MsaG014786 MtrunA17_Chr1g0197261 42.949 156 88 1 1 156 1 155 6.08e-41 137
MsaG014786 MtrunA17_Chr1g0193311 50.000 156 77 1 1 156 1 155 4.54e-40 135
MsaG014786 MtrunA17_Chr1g0188661 50.318 157 77 1 1 157 1 156 6.36e-40 135
MsaG014786 MtrunA17_Chr4g0031421 48.529 136 69 1 29 163 1 136 7.72e-39 132
MsaG014786 MtrunA17_Chr4g0012761 48.408 157 80 1 1 157 1 156 5.44e-38 130
MsaG014786 MtrunA17_Chr1g0197321 40.127 157 92 2 1 156 1 156 5.75e-37 127
MsaG014786 MtrunA17_Chr1g0197461 42.384 151 86 1 3 153 2 151 8.23e-37 127
MsaG014786 MtrunA17_Chr3g0105791 46.923 130 68 1 29 157 1 130 3.06e-36 125
MsaG014786 MtrunA17_Chr2g0296861 43.506 154 87 0 1 154 1 154 7.29e-36 124
MsaG014786 MtrunA17_Chr1g0188651 48.408 157 78 2 1 157 1 154 1.06e-34 121
MsaG014786 MtrunA17_Chr3g0106421 37.748 151 89 2 29 178 1 147 8.37e-31 113
MsaG014786 MtrunA17_Chr3g0106501 38.060 134 82 1 29 161 1 134 2.90e-27 102
MsaG014786 MtrunA17_Chr6g0457851 29.630 243 133 9 1 233 11 225 2.59e-24 99.4
MsaG014786 MtrunA17_Chr4g0008701 38.462 117 72 0 3 119 6 122 7.83e-24 99.4
MsaG014786 MtrunA17_Chr7g0267601 38.525 122 72 1 1 119 1 122 2.83e-23 97.8
MsaG014786 MtrunA17_Chr1g0212151 26.639 244 141 8 1 234 1 216 2.74e-20 88.2
MsaG014786 MtrunA17_Chr3g0128721 27.564 156 113 0 3 158 6 161 3.42e-20 89.0
MsaG014786 MtrunA17_Chr6g0449861 26.749 243 142 9 1 234 9 224 6.71e-20 87.0
MsaG014786 MtrunA17_Chr2g0296851 50.562 89 43 1 74 161 1 89 1.77e-19 80.5
MsaG014786 MtrunA17_Chr6g0449851 26.087 184 120 5 1 178 1 174 8.78e-16 75.1
MsaG014786 MtrunA17_Chr4g0022641 24.623 199 132 5 1 189 1 191 2.15e-12 65.9
MsaG014786 MtrunA17_Chr4g0059631 26.627 169 113 4 1 166 1 161 1.04e-11 62.8
MsaG014786 MtrunA17_Chr1g0212471 26.250 160 107 3 1 156 1 153 2.32e-11 60.8
MsaG014786 MtrunA17_Chr2g0317481 27.615 239 147 10 3 226 10 237 8.98e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014786 AT5G48670.1 56.164 219 83 3 1 206 1 219 2.28e-70 218
MsaG014786 AT1G65300.1 43.678 174 98 0 3 176 2 175 1.07e-50 167
MsaG014786 AT1G65330.1 47.778 180 92 1 3 180 2 181 1.50e-50 166
MsaG014786 AT3G05860.3 44.505 182 96 2 1 177 1 182 4.14e-48 159
MsaG014786 AT5G26630.1 47.120 191 99 1 1 191 1 189 4.23e-48 158
MsaG014786 AT3G05860.2 51.007 149 73 0 1 149 1 149 4.47e-48 157
MsaG014786 AT3G05860.1 41.709 199 111 2 1 194 1 199 8.56e-48 159
MsaG014786 AT5G26650.1 44.654 159 88 0 4 162 2 160 1.41e-43 150
MsaG014786 AT5G27960.1 44.025 159 89 0 4 162 2 160 1.26e-42 147
MsaG014786 AT1G31630.1 42.405 158 90 1 3 160 2 158 1.10e-41 145
MsaG014786 AT5G27810.1 66.667 93 31 0 29 121 1 93 5.75e-40 134
MsaG014786 AT1G31640.1 41.875 160 92 1 3 162 2 160 6.29e-39 140
MsaG014786 AT2G28700.1 35.714 182 108 4 1 176 1 179 6.45e-34 124
MsaG014786 AT5G26580.1 39.375 160 83 1 1 160 1 146 4.85e-32 120
MsaG014786 AT1G22590.2 35.714 154 96 1 1 154 1 151 2.22e-26 100
MsaG014786 AT2G40210.1 43.902 123 69 0 1 123 1 123 6.14e-24 99.0
MsaG014786 AT5G06500.1 37.815 119 74 0 1 119 1 119 1.37e-21 90.1
MsaG014786 AT5G55690.1 28.365 208 113 4 1 187 1 193 1.63e-17 79.7
MsaG014786 AT5G55690.2 28.365 208 113 4 1 187 1 193 1.63e-17 79.7
MsaG014786 AT5G58890.1 30.625 160 96 3 1 154 1 151 1.45e-13 68.9
MsaG014786 AT1G77950.3 37.895 95 58 1 1 95 1 94 5.26e-11 61.2
MsaG014786 AT1G77950.2 37.895 95 58 1 1 95 1 94 5.26e-11 61.2
MsaG014786 AT1G77950.1 37.895 95 58 1 1 95 1 94 5.26e-11 61.2
MsaG014786 AT1G77950.4 37.895 95 58 1 1 95 1 94 5.26e-11 61.2
MsaG014786 AT5G35120.1 32.967 91 61 0 84 174 1 91 6.82e-11 58.2

Find 55 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCCATTCTCTTCAATCTT+AGG 0.228194 3:+78491826 None:intergenic
TCTATTATCCTTTCTTTGTT+TGG 0.241148 3:+78492059 None:intergenic
GATTTGGCCAAAACCCATTT+GGG 0.290435 3:+78491680 None:intergenic
TTTATGAACTTGATGAATAA+TGG 0.322309 3:-78491742 MsaT014786.1:CDS
ATGCTTATATTGTGCATAAC+TGG 0.326699 3:+78491991 None:intergenic
GAAGAAAGGTCTAATGAAAA+AGG 0.328619 3:-78492292 MsaT014786.1:CDS
CATGGTTGATTTGAATGATA+TGG 0.333296 3:-78491971 MsaT014786.1:CDS
GAGTCATGGTCAACATGTTT+TGG 0.338044 3:-78491800 MsaT014786.1:CDS
GGATTTGGCCAAAACCCATT+TGG 0.342692 3:+78491679 None:intergenic
ACCTCTGGTTGAGGATCATA+TGG 0.368481 3:+78492216 None:intergenic
GAGGTTTGGCCGTCTCCTTT+AGG 0.389676 3:-78492198 MsaT014786.1:CDS
TGGGGATCAGGCACCTCCTA+TGG 0.395072 3:-78491716 MsaT014786.1:CDS
GAATAATGGTGGTGGGGATC+AGG 0.411629 3:-78491728 MsaT014786.1:CDS
AATGGTCGTAGCACCAACTA+TGG 0.416139 3:-78491863 MsaT014786.1:CDS
AGTATTTGAAAGATATCAAT+AGG 0.431729 3:-78491933 MsaT014786.1:CDS
ATGATCCTCAACCAGAGGTT+TGG 0.448024 3:-78492212 MsaT014786.1:CDS
GCACCAACTATGGTGGCTAA+TGG 0.475430 3:-78491853 MsaT014786.1:CDS
AAAGGAGACGGCCAAACCTC+TGG 0.476094 3:+78492201 None:intergenic
GTCTCCTTTAGGAGTTCAAA+GGG 0.477655 3:-78492187 MsaT014786.1:CDS
CGTCTCCTTTAGGAGTTCAA+AGG 0.479690 3:-78492188 MsaT014786.1:CDS
GGAAGGCAACATTCAAGAAA+AGG 0.489208 3:-78492314 MsaT014786.1:CDS
TCAATTGACCAAACAAAGAA+AGG 0.490968 3:-78492067 MsaT014786.1:CDS
TTTGAGGCAGCGGATTCAAA+AGG 0.492900 3:-78492097 MsaT014786.1:CDS
GGAGGGTTGAAATGTTGAGC+AGG 0.493102 3:-78491912 MsaT014786.1:CDS
AGGTCAAGGTCAAACTCAAA+TGG 0.513312 3:-78491881 MsaT014786.1:CDS
AGCACCCTTTGAACTCCTAA+AGG 0.514637 3:+78492183 None:intergenic
GTTGAAATGTTGAGCAGGAA+TGG 0.522645 3:-78491907 MsaT014786.1:CDS
GTACCTAAGATTGAAGAGAA+TGG 0.524167 3:-78491829 MsaT014786.1:CDS
GGGGATCAGGCACCTCCTAT+GGG 0.527561 3:-78491715 MsaT014786.1:CDS
AATCAACGACTCTGCGAGGA+AGG 0.536639 3:-78492331 MsaT014786.1:CDS
ACTCCATTAGCCACCATAGT+TGG 0.543974 3:+78491850 None:intergenic
TTTGAAAGATATCAATAGGA+GGG 0.549226 3:-78491929 MsaT014786.1:CDS
AACTTGATGAATAATGGTGG+TGG 0.552012 3:-78491736 MsaT014786.1:CDS
AGCAGGAATGGTCAAGGTCA+AGG 0.553703 3:-78491895 MsaT014786.1:CDS
GAGAATGGACAAGGGAGTCA+TGG 0.556512 3:-78491814 MsaT014786.1:CDS
ACATTCAAGAAAAGGAAGAA+AGG 0.560908 3:-78492306 MsaT014786.1:CDS
GACGGCCAAACCTCTGGTTG+AGG 0.565198 3:+78492207 None:intergenic
GGATTGACATTTCCCATAGG+AGG 0.571905 3:+78491703 None:intergenic
ATTTGGCCAAAACCCATTTG+GGG 0.587386 3:+78491681 None:intergenic
TTTGGCCAAAACCCATTTGG+GGG 0.595235 3:+78491682 None:intergenic
ATTTGAAAGATATCAATAGG+AGG 0.602830 3:-78491930 MsaT014786.1:CDS
GCATAACTGGATCAGCACTA+AGG 0.605501 3:+78492004 None:intergenic
AAGATTGAAGAGAATGGACA+AGG 0.608283 3:-78491823 MsaT014786.1:CDS
ACTTGATGAATAATGGTGGT+GGG 0.616834 3:-78491735 MsaT014786.1:CDS
ATGAACTTGATGAATAATGG+TGG 0.624781 3:-78491739 MsaT014786.1:CDS
AGTGAACTCAGTACTCTATG+TGG 0.630043 3:-78492267 MsaT014786.1:CDS
CTTGATGAATAATGGTGGTG+GGG 0.636670 3:-78491734 MsaT014786.1:CDS
CTTTGAACTCCTAAAGGAGA+CGG 0.641014 3:+78492189 None:intergenic
AGTTATGCACAATATAAGCA+TGG 0.643634 3:-78491989 MsaT014786.1:CDS
TTGATTTGAATGATATGGCA+TGG 0.644556 3:-78491966 MsaT014786.1:CDS
ATGTTGAGCAGGAATGGTCA+AGG 0.649347 3:-78491901 MsaT014786.1:CDS
GGTCGTAGCACCAACTATGG+TGG 0.652064 3:-78491860 MsaT014786.1:CDS
TCCATATGATCCTCAACCAG+AGG 0.660033 3:-78492217 MsaT014786.1:CDS
TCATAATCAACGACTCTGCG+AGG 0.671801 3:-78492335 MsaT014786.1:CDS
AGATTGAAGAGAATGGACAA+GGG 0.691703 3:-78491822 MsaT014786.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 78491666 78492385 78491666 ID=MsaG014786
Chr3 mRNA 78491666 78492385 78491666 ID=MsaT014786.1;Parent=MsaG014786
Chr3 exon 78491666 78492385 78491666 ID=MsaT014786.1.exon1;Parent=MsaT014786.1
Chr3 CDS 78491666 78492385 78491666 ID=cds.MsaT014786.1;Parent=MsaT014786.1
Gene Sequence

>MsaT014786.1

ATGACTAGAAAAAAGGTGAAACTCGCATTCATAATCAACGACTCTGCGAGGAAGGCAACATTCAAGAAAAGGAAGAAAGGTCTAATGAAAAAGGTTAGTGAACTCAGTACTCTATGTGGAATTGATGCTTGTGCTATAATCTATAGTCCATATGATCCTCAACCAGAGGTTTGGCCGTCTCCTTTAGGAGTTCAAAGGGTGCTTTCTAAGTTCCGAAAAATGCCTGAATTGGAACAAAGCAAAAAGATGGTGAACCAAGAGAGTTTTTTGAGGCAGCGGATTCAAAAGGCGAAAGATCAATTGACCAAACAAAGAAAGGATAATAGAGAAAAAGAAATGACTCAGTTAATGTTTCAGTACCTTAGTGCTGATCCAGTTATGCACAATATAAGCATGGTTGATTTGAATGATATGGCATGGTTGATTGATCAGTATTTGAAAGATATCAATAGGAGGGTTGAAATGTTGAGCAGGAATGGTCAAGGTCAAGGTCAAACTCAAATGGTCGTAGCACCAACTATGGTGGCTAATGGAGTACCTAAGATTGAAGAGAATGGACAAGGGAGTCATGGTCAACATGTTTTGGACATGAACATGGATGTTATGCAAAAGCAACATTTTTTTATGAACTTGATGAATAATGGTGGTGGGGATCAGGCACCTCCTATGGGAAATGTCAATCCCCCAAATGGGTTTTGGCCAAATCCATTTTTTCGTTGA

Protein sequence

>MsaT014786.1

MTRKKVKLAFIINDSARKATFKKRKKGLMKKVSELSTLCGIDACAIIYSPYDPQPEVWPSPLGVQRVLSKFRKMPELEQSKKMVNQESFLRQRIQKAKDQLTKQRKDNREKEMTQLMFQYLSADPVMHNISMVDLNDMAWLIDQYLKDINRRVEMLSRNGQGQGQTQMVVAPTMVANGVPKIEENGQGSHGQHVLDMNMDVMQKQHFFMNLMNNGGGDQAPPMGNVNPPNGFWPNPFFR*