AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014834

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014834 MtrunA17_Chr3g0110971 97.636 550 8 3 1 548 48 594 0.0 1107
MsaG014834 MtrunA17_Chr8g0376381 62.569 545 150 11 7 542 24 523 0.0 664
MsaG014834 MtrunA17_Chr2g0306641 46.667 540 209 12 13 541 16 487 4.34e-159 463
MsaG014834 MtrunA17_Chr7g0224271 40.842 404 192 9 164 541 291 673 2.48e-91 294
MsaG014834 MtrunA17_Chr8g0357601 35.338 399 214 12 167 542 176 553 4.96e-75 248
MsaG014834 MtrunA17_Chr5g0447691 37.436 390 207 8 170 541 174 544 7.80e-74 244
MsaG014834 MtrunA17_Chr1g0159461 36.554 383 212 8 174 541 226 592 2.23e-69 234
MsaG014834 MtrunA17_Chr3g0125811 32.870 432 248 10 136 541 155 570 7.25e-67 226
MsaG014834 MtrunA17_Chr5g0445331 35.443 395 212 10 170 541 158 532 2.44e-66 224
MsaG014834 MtrunA17_Chr3g0101141 36.504 389 212 11 170 541 172 542 2.44e-66 224
MsaG014834 MtrunA17_Chr4g0057891 34.085 399 218 11 167 541 143 520 6.86e-66 223
MsaG014834 MtrunA17_Chr4g0076091 33.681 383 224 8 173 540 175 542 8.81e-66 223
MsaG014834 MtrunA17_Chr7g0245601 37.566 378 200 11 177 543 442 794 2.96e-65 227
MsaG014834 MtrunA17_Chr2g0319091 34.872 390 215 9 170 540 209 578 1.70e-63 218
MsaG014834 MtrunA17_Chr1g0183641 35.401 387 214 10 165 540 115 476 4.54e-63 214
MsaG014834 MtrunA17_Chr4g0038761 36.340 377 206 9 177 541 88 442 4.72e-60 205
MsaG014834 MtrunA17_Chr2g0294461 29.602 402 252 6 160 542 197 586 1.11e-55 197
MsaG014834 MtrunA17_Chr2g0294471 29.750 400 251 7 160 542 191 577 2.76e-55 196
MsaG014834 MtrunA17_Chr4g0057291 32.995 394 226 11 167 540 286 661 8.42e-55 196
MsaG014834 MtrunA17_Chr4g0031701 31.340 418 254 9 140 539 222 624 2.15e-53 191
MsaG014834 MtrunA17_Chr2g0290241 29.847 392 235 9 170 541 54 425 2.54e-53 186
MsaG014834 MtrunA17_Chr4g0031731 30.900 411 251 9 147 539 229 624 7.26e-53 190
MsaG014834 MtrunA17_Chr2g0328391 31.156 398 234 6 164 540 290 668 1.58e-52 189
MsaG014834 MtrunA17_Chr4g0031711 31.387 411 249 8 148 540 337 732 1.82e-52 190
MsaG014834 MtrunA17_Chr4g0039661 34.286 385 221 12 170 541 190 555 2.01e-52 187
MsaG014834 MtrunA17_Chr5g0424131 33.985 409 210 15 167 540 115 498 1.35e-50 181
MsaG014834 MtrunA17_Chr3g0114841 31.818 418 235 12 161 547 108 506 1.41e-50 181
MsaG014834 MtrunA17_Chr7g0238891 30.612 392 236 8 168 541 367 740 9.19e-50 183
MsaG014834 MtrunA17_Chr1g0200401 32.117 411 218 17 167 541 74 459 9.91e-50 178
MsaG014834 MtrunA17_Chr4g0031721 30.233 387 242 7 168 540 311 683 2.72e-49 181
MsaG014834 MtrunA17_Chr4g0031741 30.964 394 232 10 166 540 277 649 1.20e-48 178
MsaG014834 MtrunA17_Chr2g0328341 31.122 392 240 8 163 540 262 637 3.90e-48 177
MsaG014834 MtrunA17_Chr2g0328411 29.847 392 244 8 167 544 283 657 2.93e-47 175
MsaG014834 MtrunA17_Chr2g0328431 30.155 388 240 9 167 540 283 653 3.46e-47 174
MsaG014834 MtrunA17_Chr2g0328381 30.000 390 243 8 165 540 267 640 1.54e-44 167
MsaG014834 MtrunA17_Chr7g0270031 26.835 395 262 5 160 542 176 555 2.06e-43 162
MsaG014834 MtrunA17_Chr2g0328361 29.337 392 222 6 163 540 264 614 2.53e-41 157
MsaG014834 MtrunA17_Chr5g0401131 28.894 398 247 6 166 541 107 490 2.74e-41 155
MsaG014834 MtrunA17_Chr2g0328371 35.317 252 141 6 245 490 11 246 4.74e-41 148
MsaG014834 MtrunA17_Chr2g0328401 29.167 360 217 7 166 507 283 622 4.66e-40 154
MsaG014834 MtrunA17_Chr8g0383981 32.642 386 219 14 170 540 136 495 1.30e-39 150
MsaG014834 MtrunA17_Chr1g0194621 25.672 409 258 9 159 542 132 519 2.84e-37 144
MsaG014834 MtrunA17_Chr5g0396491 34.685 222 131 3 161 373 44 260 4.12e-36 140
MsaG014834 MtrunA17_Chr5g0396491 38.182 165 89 3 377 541 327 478 2.93e-30 124
MsaG014834 MtrunA17_Chr4g0038851 33.463 257 149 6 127 373 8 252 3.45e-35 137
MsaG014834 MtrunA17_Chr4g0038851 36.364 165 92 2 377 541 318 469 3.61e-28 117
MsaG014834 MtrunA17_Chr7g0236851 29.187 418 250 12 143 540 265 656 5.63e-35 139
MsaG014834 MtrunA17_Chr6g0468561 30.256 390 231 11 172 541 294 662 5.95e-35 139
MsaG014834 MtrunA17_Chr7g0230771 28.684 380 243 8 170 541 372 731 5.54e-34 137
MsaG014834 MtrunA17_Chr7g0230731 28.947 380 242 8 170 541 369 728 3.49e-33 134
MsaG014834 MtrunA17_Chr4g0012071 26.649 379 243 9 174 541 197 551 2.62e-31 127
MsaG014834 MtrunA17_Chr3g0085361 41.615 161 79 4 382 542 206 351 1.05e-29 120
MsaG014834 MtrunA17_Chr3g0085361 33.577 137 86 2 237 373 13 144 2.66e-20 92.4
MsaG014834 MtrunA17_Chr5g0401421 25.935 428 238 11 174 540 36 445 3.81e-29 120
MsaG014834 MtrunA17_Chr2g0329591 27.342 395 242 9 174 541 75 451 3.85e-29 120
MsaG014834 MtrunA17_Chr3g0085681 39.130 161 84 2 382 542 291 437 1.26e-28 118
MsaG014834 MtrunA17_Chr3g0085681 30.622 209 131 3 174 373 26 229 1.50e-28 118
MsaG014834 MtrunA17_Chr4g0053901 25.352 426 241 11 174 540 60 467 2.85e-28 117
MsaG014834 MtrunA17_Chr8g0373561 26.943 386 245 13 170 541 179 541 9.58e-25 108
MsaG014834 MtrunA17_Chr4g0068471 53.333 105 39 2 160 264 23 117 1.60e-24 98.6
MsaG014834 MtrunA17_Chr8g0344101 24.372 398 252 10 174 542 224 601 2.66e-23 103
MsaG014834 MtrunA17_Chr2g0323111 27.778 162 102 2 379 540 159 305 3.37e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014834 AT2G01570.1 60.832 577 168 10 1 542 29 582 0.0 669
MsaG014834 AT3G03450.1 63.586 541 145 11 15 542 45 546 0.0 665
MsaG014834 AT1G14920.1 60.573 558 158 7 6 545 20 533 0.0 662
MsaG014834 AT1G66350.1 61.111 540 151 10 10 546 28 511 0.0 646
MsaG014834 AT5G17490.1 58.256 539 162 12 13 542 33 517 0.0 588
MsaG014834 AT5G48150.1 37.984 387 202 9 174 541 123 490 1.05e-74 245
MsaG014834 AT5G48150.4 37.984 387 202 9 174 541 123 490 1.05e-74 245
MsaG014834 AT5G48150.2 37.984 387 202 9 174 541 123 490 1.05e-74 245
MsaG014834 AT5G48150.3 37.984 387 202 9 174 541 123 490 1.05e-74 245
MsaG014834 AT3G54220.1 36.047 430 234 11 122 540 250 649 9.69e-69 234
MsaG014834 AT2G04890.1 32.787 427 240 9 138 541 11 413 2.75e-67 224
MsaG014834 AT1G50600.3 35.065 385 217 10 173 541 159 526 8.58e-67 226
MsaG014834 AT1G50600.2 35.065 385 217 10 173 541 234 601 3.35e-66 226
MsaG014834 AT1G50600.1 35.065 385 217 10 173 541 230 597 3.69e-66 226
MsaG014834 AT5G41920.1 36.061 391 205 11 168 541 38 400 1.97e-64 216
MsaG014834 AT1G21450.1 33.766 385 225 8 172 541 224 593 1.15e-60 211
MsaG014834 AT4G17230.1 34.103 390 220 7 170 541 155 525 2.17e-59 206
MsaG014834 AT4G17230.2 34.103 390 220 7 170 541 155 525 2.17e-59 206
MsaG014834 AT5G59450.1 32.134 389 237 8 168 541 222 598 8.61e-56 198
MsaG014834 AT1G07530.1 31.818 396 237 10 162 540 385 764 1.64e-52 191
MsaG014834 AT3G46600.2 30.971 381 236 7 174 541 5 371 4.63e-51 179
MsaG014834 AT1G63100.1 31.328 399 227 12 170 540 249 628 9.99e-51 184
MsaG014834 AT1G63100.2 31.328 399 227 12 170 540 274 653 1.71e-50 184
MsaG014834 AT1G55580.1 32.267 344 204 6 211 541 118 445 2.99e-50 179
MsaG014834 AT3G46600.1 31.008 387 240 8 168 541 207 579 4.87e-50 181
MsaG014834 AT3G46600.3 30.749 387 241 7 168 541 175 547 5.42e-50 181
MsaG014834 AT1G07520.2 29.275 386 242 7 174 540 155 528 5.58e-48 175
MsaG014834 AT1G07520.4 29.275 386 242 7 174 540 155 528 5.58e-48 175
MsaG014834 AT5G66770.1 33.854 384 226 11 170 541 217 584 5.88e-48 176
MsaG014834 AT1G07520.1 29.275 386 242 7 174 540 319 692 2.29e-47 176
MsaG014834 AT1G07520.3 29.275 386 242 7 174 540 320 693 2.29e-47 176
MsaG014834 AT2G29060.2 30.026 383 239 9 174 540 285 654 1.19e-46 173
MsaG014834 AT2G29060.1 30.026 383 239 9 174 540 322 691 1.80e-46 173
MsaG014834 AT2G29065.1 28.682 387 244 5 174 540 253 627 1.45e-45 170
MsaG014834 AT2G37650.2 30.077 389 240 9 168 541 330 701 6.23e-45 169
MsaG014834 AT2G37650.1 30.077 389 240 9 168 541 342 713 7.94e-45 169
MsaG014834 AT4G08250.1 31.000 400 240 15 164 544 98 480 8.23e-44 162
MsaG014834 AT3G50650.1 33.141 347 207 9 207 541 209 542 1.89e-43 162
MsaG014834 AT4G37650.1 28.392 398 240 7 174 541 147 529 2.10e-43 162
MsaG014834 AT5G52510.1 29.146 398 230 9 172 541 267 640 4.77e-41 157
MsaG014834 AT1G50420.1 34.711 242 136 5 143 373 33 263 5.96e-35 137
MsaG014834 AT1G50420.1 36.527 167 91 3 376 541 327 479 3.51e-28 118
MsaG014834 AT3G49950.1 27.020 396 241 11 174 540 31 407 1.68e-30 124
MsaG014834 AT4G00150.1 27.273 407 242 13 146 541 191 554 1.29e-29 123
MsaG014834 AT3G60630.1 29.747 316 186 12 239 541 330 622 8.05e-28 118
MsaG014834 AT4G36710.1 25.831 391 252 11 167 542 119 486 1.82e-27 116
MsaG014834 AT2G45160.1 27.500 360 216 10 200 541 307 639 1.12e-25 112
MsaG014834 AT3G13840.1 25.455 385 247 11 174 541 149 510 1.00e-19 92.8

Find 142 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGACCCTTTAGCTTCTT+TGG 0.137522 3:+79095940 MsaT014834.1:CDS
TTGCTGGTGGTGATGGTTAT+AGG 0.196762 3:+79097217 MsaT014834.1:CDS
TCGTCTTGTTCATACTTTAA+TGG 0.200580 3:+79096192 MsaT014834.1:CDS
AGAGTTCATGTTATTGATTT+TGG 0.230040 3:+79096493 MsaT014834.1:CDS
TTCGCTAGAGGGTTCGAATT+CGG 0.258385 3:+79096957 MsaT014834.1:CDS
TCGCTAGAGGGTTCGAATTC+GGG 0.265073 3:+79096958 MsaT014834.1:CDS
GATGAATCGATTGTTTCTTC+TGG 0.269432 3:-79096364 None:intergenic
TGGGTTCGAACCGGTTCATT+TGG 0.283884 3:+79097152 MsaT014834.1:CDS
AACTCGAACTCGACCGGGTT+GGG 0.288531 3:+79096994 MsaT014834.1:CDS
TGCTGGTGGTGATGGTTATA+GGG 0.290318 3:+79097218 MsaT014834.1:CDS
ACTTTGTTAGCTTTGTTTGC+TGG 0.304267 3:+79097201 MsaT014834.1:CDS
AATAATGGGTGTCTTATGTT+AGG 0.308660 3:+79097249 MsaT014834.1:CDS
GGTCGGATCTCAAGCATATT+CGG 0.315210 3:-79096733 None:intergenic
TATAGGGTTGAGGAAAATAA+TGG 0.321686 3:+79097234 MsaT014834.1:CDS
AGCAAGTGCTTGTGCGAAAT+AGG 0.327440 3:-79096323 None:intergenic
AATGACCCTTTAGCTTCTTT+GGG 0.347195 3:+79095941 MsaT014834.1:CDS
TGAACAGCTTGAGATGGTTA+TGG 0.351890 3:+79095796 MsaT014834.1:CDS
GACATCGCCACCGCCGGTTC+CGG 0.353495 3:-79096103 None:intergenic
CGGTCTAGGATGGGTTCAGC+TGG 0.354100 3:+79097132 MsaT014834.1:CDS
ATCAATAACATGAACTCTTC+CGG 0.369829 3:-79096488 None:intergenic
GGGTTCGAACCGGTTCATTT+GGG 0.373116 3:+79097153 MsaT014834.1:CDS
GAGAACTTTCTCAACTGAAC+CGG 0.373578 3:-79096812 None:intergenic
CTTTGACTCAATGGCGGTCT+AGG 0.374930 3:+79097118 MsaT014834.1:CDS
CGGGTTGGGTTCACCGAGTC+AGG 0.389915 3:+79097008 MsaT014834.1:CDS
GAACAGCTTGAGATGGTTAT+GGG 0.390884 3:+79095797 MsaT014834.1:CDS
ATGAATCGATTGTTTCTTCT+GGG 0.391052 3:-79096363 None:intergenic
CTTGTTGATACACAAGAAAC+TGG 0.397184 3:+79096166 MsaT014834.1:CDS
GGTGGGTATGCAGCCATTCC+AGG 0.406758 3:-79096034 None:intergenic
TATGATCTTAGCGCTATTCC+TGG 0.412789 3:+79096016 MsaT014834.1:CDS
TCATTGATTTGAGAAGAATC+AGG 0.415906 3:-79095923 None:intergenic
GACTCAATGGCGGTCTAGGA+TGG 0.416785 3:+79097122 MsaT014834.1:CDS
GCGGTCCAATACCGGTTAAC+CGG 0.417218 3:-79096588 None:intergenic
CTCGAAGCCTTCGCCGGTGC+CGG 0.419305 3:+79096469 MsaT014834.1:CDS
ACGTTCCGGTTAACCGGTAT+TGG 0.428730 3:+79096583 MsaT014834.1:CDS
GACGAGTTGTTAGCAGCATT+AGG 0.430561 3:+79095728 MsaT014834.1:CDS
GTCTGAACCGGAACCGGCGG+TGG 0.430629 3:+79096096 MsaT014834.1:CDS
ACCCAACCGATCTATATTCA+TGG 0.435726 3:+79095873 MsaT014834.1:CDS
GTCGGATCTCAAGCATATTC+GGG 0.436067 3:-79096732 None:intergenic
GACTTGGTCTGAACCGGAAC+CGG 0.436364 3:+79096090 MsaT014834.1:CDS
CCTCCTACGTTCCGGTTAAC+CGG 0.440392 3:+79096577 MsaT014834.1:CDS
TTCTCCATCACCGCCGGTGG+TGG 0.440610 3:-79096127 None:intergenic
CGCCTGCGGCGGTCCAATAC+CGG 0.444818 3:-79096596 None:intergenic
TTATTAGCTTCATTACAAAC+TGG 0.451250 3:+79096280 MsaT014834.1:CDS
GCTTTGTTTGCTGGTGGTGA+TGG 0.452468 3:+79097210 MsaT014834.1:CDS
AATGAAGACTTGGTCTGAAC+CGG 0.458221 3:+79096084 MsaT014834.1:CDS
GTTAACAGCAACAGCTTCAC+CGG 0.459180 3:-79096755 None:intergenic
GCATTTGAACCCAAATGAAC+CGG 0.461370 3:-79097162 None:intergenic
AATGTAAAGCTTCAGTAAAC+CGG 0.462320 3:-79096915 None:intergenic
TTTCTCAACTGAACCGGGTC+GGG 0.464834 3:-79096806 None:intergenic
CACTCATTGCTACTTCTGCT+TGG 0.477277 3:+79097286 MsaT014834.1:CDS
CAGCTAAGCGAATGAAGACT+TGG 0.478448 3:+79096074 MsaT014834.1:CDS
AGTTTATTCGATTCGCTAGA+GGG 0.479255 3:+79096946 MsaT014834.1:CDS
AACTCTTCCGGCACCGGCGA+AGG 0.479429 3:-79096476 None:intergenic
GGTGTTCAGTTCGAATTTCG+TGG 0.481901 3:+79096679 MsaT014834.1:CDS
GGGTTCAGCTGGGTTCGAAC+CGG 0.481901 3:+79097143 MsaT014834.1:CDS
CAAGCGCTGGCATTACGCCC+CGG 0.482592 3:+79096550 MsaT014834.1:CDS
GTCACCACCACCGGCGGTGA+TGG 0.483554 3:+79096123 MsaT014834.1:CDS
ACCCATGAATATAGATCGGT+TGG 0.487659 3:-79095875 None:intergenic
GACATGAGACTTTGACTCAA+TGG 0.488320 3:+79097109 MsaT014834.1:CDS
TTGATGTCAGAGATTTATCT+CGG 0.494129 3:+79097036 MsaT014834.1:CDS
GCCAGCGCTTATGCAAGCGC+TGG 0.499517 3:+79096537 MsaT014834.1:CDS
AATTCAAACTCGAACTCGAC+CGG 0.499871 3:+79096988 MsaT014834.1:CDS
GTTAGCAGCATTAGGTTACA+AGG 0.501092 3:+79095736 MsaT014834.1:CDS
CAAGGTTCGTTCTTCAGACA+TGG 0.505343 3:+79095754 MsaT014834.1:CDS
GCCAGCGCTTGCATAAGCGC+TGG 0.505414 3:-79096538 None:intergenic
ATAATGGGTGTCTTATGTTA+GGG 0.505618 3:+79097250 MsaT014834.1:CDS
GAAGAACCCAAAGAAGCTAA+AGG 0.507363 3:-79095947 None:intergenic
AGAAACCAACGGTGGTGGAA+TGG 0.507648 3:+79095706 MsaT014834.1:CDS
ATAGGGTTGAGGAAAATAAT+GGG 0.509069 3:+79097235 MsaT014834.1:CDS
TTATGTTAGGGTGGCATACA+AGG 0.510723 3:+79097262 MsaT014834.1:CDS
AACATGAACTCTTCCGGCAC+CGG 0.512483 3:-79096482 None:intergenic
CATCATTAAAAGTGTTGTTG+AGG 0.512754 3:-79095985 None:intergenic
GTCTTAAACAAGGGATGCAA+TGG 0.515161 3:+79096515 MsaT014834.1:CDS
ATGCTTTGCAACAAGTGGGC+TGG 0.515330 3:+79096629 MsaT014834.1:CDS
CGGAACGTAGGAGGACCGCC+GGG 0.517761 3:-79096568 None:intergenic
GTAAACCGGTCTACGAAAAC+CGG 0.519994 3:-79096901 None:intergenic
GCTCAGCTTGCTCAGACAAT+CGG 0.520538 3:+79096658 MsaT014834.1:CDS
GAGTTTATTCGATTCGCTAG+AGG 0.521091 3:+79096945 MsaT014834.1:CDS
AAGAACCCAAAGAAGCTAAA+GGG 0.524765 3:-79095946 None:intergenic
AAACTCGAACTCGACCGGGT+TGG 0.526219 3:+79096993 MsaT014834.1:CDS
TGGTGATGGTTATAGGGTTG+AGG 0.526589 3:+79097224 MsaT014834.1:CDS
CCGGCGGTCCTCCTACGTTC+CGG 0.526841 3:+79096569 MsaT014834.1:CDS
GGTGGTGACATCGCCACCGC+CGG 0.527720 3:-79096109 None:intergenic
TACCGATGCTTTGCAACAAG+TGG 0.527843 3:+79096624 MsaT014834.1:CDS
CATTGATTTGAGAAGAATCA+GGG 0.530327 3:-79095922 None:intergenic
AACCGGTATTGGACCGCCGC+AGG 0.530912 3:+79096594 MsaT014834.1:CDS
AGAACTTTCTCAACTGAACC+GGG 0.532923 3:-79096811 None:intergenic
ACTCAATGGCGGTCTAGGAT+GGG 0.535484 3:+79097123 MsaT014834.1:CDS
GAACAGTATCAGAAGAAAGA+TGG 0.536773 3:-79095844 None:intergenic
AGGGTTGAGTTCAGTGAGCA+TGG 0.538956 3:-79095903 None:intergenic
GGTGGCGATGTCACCACCAC+CGG 0.546557 3:+79096114 MsaT014834.1:CDS
CCGGTTAACCGGAACGTAGG+AGG 0.547199 3:-79096577 None:intergenic
TGGCTTATGAAGGAGTGGAC+CGG 0.547664 3:+79097079 MsaT014834.1:CDS
GAACAAGAAGCGAATCATAA+CGG 0.547991 3:+79096877 MsaT014834.1:CDS
CCGGAACGTAGGAGGACCGC+CGG 0.550263 3:-79096569 None:intergenic
CATACTATGTTAGCCCGACC+CGG 0.551742 3:+79096793 MsaT014834.1:CDS
ATGGGTAGTGCTCAAGAAGA+AGG 0.552354 3:+79095815 MsaT014834.1:CDS
GTACCCTGAGTTAACTCACT+TGG 0.552518 3:-79097328 None:intergenic
GCCCACTTGTTGCAAAGCAT+CGG 0.552582 3:-79096626 None:intergenic
CTCGTCCATTCCACCACCGT+TGG 0.556294 3:-79095711 None:intergenic
GGTCTAGGATGGGTTCAGCT+GGG 0.557426 3:+79097133 MsaT014834.1:CDS
TTGTTAGCTTTGTTTGCTGG+TGG 0.558150 3:+79097204 MsaT014834.1:CDS
TGTAATGTGGTGGCTTATGA+AGG 0.560033 3:+79097069 MsaT014834.1:CDS
GGCTTATGAAGGAGTGGACC+GGG 0.562784 3:+79097080 MsaT014834.1:CDS
AAATGAGCGAATTTGAGATA+AGG 0.566361 3:-79096427 None:intergenic
AAAGCTTGAACAGCTTGAGA+TGG 0.568021 3:+79095790 MsaT014834.1:CDS
GCTATTCTCGAAGCCTTCGC+CGG 0.569462 3:+79096463 MsaT014834.1:CDS
CCCAACCGATCTATATTCAT+GGG 0.569926 3:+79095874 MsaT014834.1:CDS
TGATGTCAGAGATTTATCTC+GGG 0.573037 3:+79097037 MsaT014834.1:CDS
TTGAACCCATGAATATAGAT+CGG 0.574469 3:-79095879 None:intergenic
ATTTGTTTGTAACAGTATTG+CGG 0.580712 3:+79096702 MsaT014834.1:CDS
ATTCAAACTCGAACTCGACC+GGG 0.585112 3:+79096989 MsaT014834.1:CDS
TCTTAGCGCTATTCCTGGAA+TGG 0.585827 3:+79096021 MsaT014834.1:CDS
CTTGTATTCTCCATCACCGC+CGG 0.586626 3:-79096133 None:intergenic
GACATCAACAAATCCTGACT+CGG 0.588551 3:-79097021 None:intergenic
CTTTCTCAACTGAACCGGGT+CGG 0.589796 3:-79096807 None:intergenic
GCACTTGCTAGAAGAATCTA+CGG 0.594505 3:+79096337 MsaT014834.1:CDS
TCGGTATTGTCCGCCTGCGG+CGG 0.603762 3:-79096607 None:intergenic
CCCATGAATATAGATCGGTT+GGG 0.605216 3:-79095874 None:intergenic
CTGACTCGGTGAACCCAACC+CGG 0.607910 3:-79097007 None:intergenic
TTGGTCTGAACCGGAACCGG+CGG 0.617912 3:+79096093 MsaT014834.1:CDS
GTATTCTCCATCACCGCCGG+TGG 0.619409 3:-79096130 None:intergenic
ACCGATGCTTTGCAACAAGT+GGG 0.624749 3:+79096625 MsaT014834.1:CDS
TGTGTATCAACAAGAACCAC+CGG 0.627324 3:-79096157 None:intergenic
AATATGCTTGAGATCCGACC+CGG 0.629333 3:+79096736 MsaT014834.1:CDS
GGCGATGTCACCACCACCGG+CGG 0.632737 3:+79096117 MsaT014834.1:CDS
CGGTATTGGACCGCCGCAGG+CGG 0.635024 3:+79096597 MsaT014834.1:CDS
GCGCTGGCATTACGCCCCGG+CGG 0.637073 3:+79096553 MsaT014834.1:CDS
AAGTCTCATGTCTTTCAACC+CGG 0.637365 3:-79097098 None:intergenic
ATGGGTGTCTTATGTTAGGG+TGG 0.639065 3:+79097253 MsaT014834.1:CDS
GCATCGGTATTGTCCGCCTG+CGG 0.640917 3:-79096610 None:intergenic
CGGGAGACAGATATGTAATG+TGG 0.642807 3:+79097056 MsaT014834.1:CDS
CAGCAACAGCTTCACCGGGT+CGG 0.649987 3:-79096750 None:intergenic
TTAACAGCAACAGCTTCACC+GGG 0.654245 3:-79096754 None:intergenic
GGTGATGGAGAATACAAGAC+CGG 0.654883 3:+79096138 MsaT014834.1:CDS
GAGACAGATATGTAATGTGG+TGG 0.655986 3:+79097059 MsaT014834.1:CDS
TGTGGTGGCTTATGAAGGAG+TGG 0.663059 3:+79097074 MsaT014834.1:CDS
AGAAGCGAATCATAACGGAC+CGG 0.675008 3:+79096882 MsaT014834.1:CDS
ATGAGACTTTGACTCAATGG+CGG 0.698553 3:+79097112 MsaT014834.1:CDS
GATGGAGAATACAAGACCGG+TGG 0.704648 3:+79096141 MsaT014834.1:CDS
ATACCGGTTAACCGGAACGT+AGG 0.707495 3:-79096580 None:intergenic
GGAACGTAGGAGGACCGCCG+GGG 0.728118 3:-79096567 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 79095689 79097335 79095689 ID=MsaG014834
Chr3 mRNA 79095689 79097335 79095689 ID=MsaT014834.1;Parent=MsaG014834
Chr3 exon 79095689 79097335 79095689 ID=MsaT014834.1.exon1;Parent=MsaT014834.1
Chr3 CDS 79095689 79097335 79095689 ID=cds.MsaT014834.1;Parent=MsaT014834.1
Gene Sequence

>MsaT014834.1

ATGTGGAGAGAAGAAAAAGAAACCAACGGTGGTGGAATGGACGAGTTGTTAGCAGCATTAGGTTACAAGGTTCGTTCTTCAGACATGGCTGATGTAGCTCAAAAGCTTGAACAGCTTGAGATGGTTATGGGTAGTGCTCAAGAAGAAGGTATCAACCATCTTTCTTCTGATACTGTTCACTATGACCCAACCGATCTATATTCATGGGTTCAAACCATGCTCACTGAACTCAACCCTGATTCTTCTCAAATCAATGACCCTTTAGCTTCTTTGGGTTCTTCTTCTTCTTCTTCAATCCTCAACAACACTTTTAATGATGATTCTGAGTATGATCTTAGCGCTATTCCTGGAATGGCTGCATACCCACCTCAAGAAGAAAACATAACAGCTAAGCGAATGAAGACTTGGTCTGAACCGGAACCGGCGGTGGCGATGTCACCACCACCGGCGGTGATGGAGAATACAAGACCGGTGGTTCTTGTTGATACACAAGAAACTGGTGTTCGTCTTGTTCATACTTTAATGGCTTGTGCAGAAGCGATTCAGCAAGAGAATCTGAAACTTGCAGAAGCGCTTGTGAAGCATATAAGCTTATTAGCTTCATTACAAACTGGTGCGATGAGAAAAGTTGCTTCCTATTTCGCACAAGCACTTGCTAGAAGAATCTACGGTAACCCAGAAGAAACAATCGATTCATCATTCTCAGAAATTCTTCACATGCATTTTTACGAATCTTCACCTTATCTCAAATTCGCTCATTTCACTGCTAATCAAGCTATTCTCGAAGCCTTCGCCGGTGCCGGAAGAGTTCATGTTATTGATTTTGGTCTTAAACAAGGGATGCAATGGCCAGCGCTTATGCAAGCGCTGGCATTACGCCCCGGCGGTCCTCCTACGTTCCGGTTAACCGGTATTGGACCGCCGCAGGCGGACAATACCGATGCTTTGCAACAAGTGGGCTGGAAATTAGCTCAGCTTGCTCAGACAATCGGTGTTCAGTTCGAATTTCGTGGATTTGTTTGTAACAGTATTGCGGATCTTGACCCGAATATGCTTGAGATCCGACCCGGTGAAGCTGTTGCTGTTAACTCTGTTTTTGAGCTTCATACTATGTTAGCCCGACCCGGTTCAGTTGAGAAAGTTCTCAACACTGTTAAGAAGATTAACCCTAAAATCGTAACAATCGTTGAACAAGAAGCGAATCATAACGGACCGGTTTTCGTAGACCGGTTTACTGAAGCTTTACATTATTACTCGAGTTTATTCGATTCGCTAGAGGGTTCGAATTCGGGTTTGAATAATTCAAACTCGAACTCGACCGGGTTGGGTTCACCGAGTCAGGATTTGTTGATGTCAGAGATTTATCTCGGGAGACAGATATGTAATGTGGTGGCTTATGAAGGAGTGGACCGGGTTGAAAGACATGAGACTTTGACTCAATGGCGGTCTAGGATGGGTTCAGCTGGGTTCGAACCGGTTCATTTGGGTTCAAATGCGTTTAAACAAGCTAGTACTTTGTTAGCTTTGTTTGCTGGTGGTGATGGTTATAGGGTTGAGGAAAATAATGGGTGTCTTATGTTAGGGTGGCATACAAGGTCACTCATTGCTACTTCTGCTTGGAAGCTTCCACAAAATGAGTCCAAGTGA
Protein sequence

>MsaT014834.1

MWREEKETNGGGMDELLAALGYKVRSSDMADVAQKLEQLEMVMGSAQEEGINHLSSDTVHYDPTDLYSWVQTMLTELNPDSSQINDPLASLGSSSSSSILNNTFNDDSEYDLSAIPGMAAYPPQEENITAKRMKTWSEPEPAVAMSPPPAVMENTRPVVLVDTQETGVRLVHTLMACAEAIQQENLKLAEALVKHISLLASLQTGAMRKVASYFAQALARRIYGNPEETIDSSFSEILHMHFYESSPYLKFAHFTANQAILEAFAGAGRVHVIDFGLKQGMQWPALMQALALRPGGPPTFRLTGIGPPQADNTDALQQVGWKLAQLAQTIGVQFEFRGFVCNSIADLDPNMLEIRPGEAVAVNSVFELHTMLARPGSVEKVLNTVKKINPKIVTIVEQEANHNGPVFVDRFTEALHYYSSLFDSLEGSNSGLNNSNSNSTGLGSPSQDLLMSEIYLGRQICNVVAYEGVDRVERHETLTQWRSRMGSAGFEPVHLGSNAFKQASTLLALFAGGDGYRVEENNGCLMLGWHTRSLIATSAWKLPQNESK*