AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014932


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014932 MtrunA17_Chr3g0112181 93.462 260 16 1 1 259 1 260 0.0 511
MsaG014932 MtrunA17_Chr5g0435711 47.674 258 93 13 8 259 11 232 1.06e-51 168
MsaG014932 MtrunA17_Chr4g0009461 57.282 103 30 3 33 121 36 138 4.64e-30 114
MsaG014932 MtrunA17_Chr5g0400571 71.930 57 15 1 33 89 59 114 2.79e-23 95.1
MsaG014932 MtrunA17_Chr2g0315591 49.398 83 39 1 10 89 2 84 2.32e-19 84.0
MsaG014932 MtrunA17_Chr1g0184381 80.952 42 8 0 49 90 29 70 7.40e-18 79.0
MsaG014932 MtrunA17_Chr7g0239441 70.000 50 15 0 55 104 37 86 6.69e-16 72.8
MsaG014932 MtrunA17_Chr4g0032111 62.500 48 18 0 54 101 20 67 7.96e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014932 AT2G37740.1 75.439 57 11 1 33 89 18 71 5.93e-24 98.2
MsaG014932 AT4G17810.1 30.804 224 113 4 30 253 19 200 1.23e-23 95.1
MsaG014932 AT5G06070.1 37.295 244 104 10 25 259 22 225 6.59e-23 94.0
MsaG014932 AT4G17810.2 69.091 55 17 0 30 84 19 73 1.09e-22 92.0
MsaG014932 AT3G23130.1 82.927 41 7 0 47 87 37 77 5.00e-18 80.1
MsaG014932 AT2G42410.2 78.049 41 9 0 48 88 40 80 3.05e-17 78.6
MsaG014932 AT2G42410.1 78.049 41 9 0 48 88 40 80 3.05e-17 78.6
MsaG014932 AT2G42410.3 78.049 41 9 0 48 88 66 106 3.27e-17 79.0
MsaG014932 AT3G09290.1 63.636 44 16 0 55 98 33 76 1.19e-12 65.1
MsaG014932 AT3G53820.1 68.571 35 11 0 55 89 19 53 3.88e-11 60.1

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTCTTCTTCAATTAGA+TGG 0.176609 3:-80405710 MsaT014932.1:CDS
TGTTGAAACAAGTTTGTCTA+TGG 0.217128 3:-80405598 MsaT014932.1:CDS
TTCCCAAGACTTGTTGTTGA+TGG 0.233876 3:+80406097 None:intergenic
TTTAGGTCTGCTCAAGCTTT+AGG 0.265540 3:-80405984 MsaT014932.1:CDS
GCTTAGCTCTATCTCTTCTA+TGG 0.294197 3:+80405946 None:intergenic
AATGAAGTTCTAATTCATTA+TGG 0.297493 3:+80405904 None:intergenic
GGACAAAATAATTCAATGTT+TGG 0.329063 3:-80405576 MsaT014932.1:CDS
AGATCCAAGTCTTCTATTCC+AGG 0.334055 3:+80405387 None:intergenic
GGATACTTGGTGGATGTATA+TGG 0.348621 3:-80406046 MsaT014932.1:CDS
GAAGAAATGGAAGCTGTCTT+TGG 0.357856 3:+80405492 None:intergenic
GAAGAGTTATCTCTGCTTGT+TGG 0.403326 3:+80405789 None:intergenic
CAGTCTGCAGAGTTTGTGGT+TGG 0.405826 3:-80405417 MsaT014932.1:CDS
TAAATATGATGTGGATGAAA+AGG 0.412314 3:-80406166 MsaT014932.1:CDS
GACAGGCAGTGAAGAAGAAA+TGG 0.412947 3:+80405479 None:intergenic
TCTTGTGTAGATGCAGTTGA+TGG 0.423703 3:+80405759 None:intergenic
GAAAATTTGTTCCCTTGCAT+TGG 0.427727 3:+80405644 None:intergenic
AATGAAGGAGTGCCATCATT+TGG 0.429411 3:-80406122 MsaT014932.1:CDS
GAAGAATATGAAGACATGTT+TGG 0.429891 3:+80405723 None:intergenic
ATATTCTTCTTCAATTAGAT+GGG 0.438910 3:-80405709 MsaT014932.1:CDS
GAAGATGCAGCTAGGATACT+TGG 0.443307 3:-80406059 MsaT014932.1:CDS
GAACCATCAACAACAAGTCT+TGG 0.447185 3:-80406100 MsaT014932.1:CDS
ACTTCTGTAAAAGAGAGTTT+AGG 0.451370 3:-80406001 MsaT014932.1:CDS
TTGGATCTTGAACTCAGGCT+TGG 0.454061 3:-80405372 MsaT014932.1:CDS
TGGATCTTGAACTCAGGCTT+GGG 0.459255 3:-80405371 MsaT014932.1:CDS
AAACATGGCTTCAGCAAGAC+AGG 0.470524 3:+80405462 None:intergenic
AAGTGACATGAGTAAGATCT+TGG 0.483411 3:+80406020 None:intergenic
TTTGTGGTTGGAGTTAAGCC+TGG 0.483977 3:-80405405 MsaT014932.1:CDS
TTGTTGATGGTTCCAAATGA+TGG 0.490417 3:+80406110 None:intergenic
AACCATCAACAACAAGTCTT+GGG 0.510991 3:-80406099 MsaT014932.1:CDS
GTTGAAACAAGTTTGTCTAT+GGG 0.512192 3:-80405597 MsaT014932.1:CDS
AAGACTTGGATCTTGAACTC+AGG 0.538252 3:-80405377 MsaT014932.1:CDS
GAGTCTCTTGATTCTGAACA+AGG 0.543085 3:-80405672 MsaT014932.1:CDS
AGGTCTGCTCAAGCTTTAGG+TGG 0.544624 3:-80405981 MsaT014932.1:CDS
AACAAGGGCTAGCCAATGCA+AGG 0.549159 3:-80405656 MsaT014932.1:CDS
TAAGCCTGGAATAGAAGACT+TGG 0.563435 3:-80405391 MsaT014932.1:CDS
ATCTTGCACTCACACAATGA+AGG 0.564898 3:-80406137 MsaT014932.1:CDS
GCAGAGATAACTCTTCTACT+TGG 0.579663 3:-80405782 MsaT014932.1:CDS
TATTCTTCTTCAATTAGATG+GGG 0.582180 3:-80405708 MsaT014932.1:CDS
GATGCAGCTAGGATACTTGG+TGG 0.596955 3:-80406056 MsaT014932.1:CDS
TGACATGAGTAAGATCTTGG+AGG 0.599612 3:+80406023 None:intergenic
ACTGATGATGATTCATGTGG+TGG 0.616045 3:-80405540 MsaT014932.1:CDS
CTGATGATGATTCATGTGGT+GGG 0.621213 3:-80405539 MsaT014932.1:CDS
ATTCTTCTTCAATTAGATGG+GGG 0.625385 3:-80405707 MsaT014932.1:CDS
AGGAATCACTAAATATGATG+TGG 0.652487 3:-80406175 MsaT014932.1:CDS
AAACATCTATCATTCAAACA+TGG 0.662081 3:+80405447 None:intergenic
TGATGATGATTCATGTGGTG+GGG 0.671193 3:-80405538 MsaT014932.1:CDS
ACAAGGGCTAGCCAATGCAA+GGG 0.692695 3:-80405655 MsaT014932.1:CDS
AGTCTCTTGATTCTGAACAA+GGG 0.707551 3:-80405671 MsaT014932.1:CDS
CAAACTGATGATGATTCATG+TGG 0.716039 3:-80405543 MsaT014932.1:CDS
CAGAATCAAGAGACTCCATG+TGG 0.748859 3:+80405679 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 80405350 80406201 80405350 ID=MsaG014932
Chr3 mRNA 80405350 80406201 80405350 ID=MsaT014932.1;Parent=MsaG014932
Chr3 exon 80405350 80405813 80405350 ID=MsaT014932.1.exon2;Parent=MsaT014932.1
Chr3 CDS 80405350 80405813 80405350 ID=cds.MsaT014932.1;Parent=MsaT014932.1
Chr3 exon 80405886 80406201 80405886 ID=MsaT014932.1.exon1;Parent=MsaT014932.1
Chr3 CDS 80405886 80406201 80405886 ID=cds.MsaT014932.1;Parent=MsaT014932.1
Gene Sequence

>MsaT014932.1

ATGGAGGAATCACTAAATATGATGTGGATGAAAAGGAAACAAATCTTGCACTCACACAATGAAGGAGTGCCATCATTTGGAACCATCAACAACAAGTCTTGGGAAGAAAAAGCTTTTGCAGAAGATGCAGCTAGGATACTTGGTGGATGTATATGGCCTCCAAGATCTTACTCATGTCACTTCTGTAAAAGAGAGTTTAGGTCTGCTCAAGCTTTAGGTGGTCACATGAATGTCCATAGAAGAGATAGAGCTAAGCTCAAACAAAGTCTTAGCAACCATAATGAATTAGAACTTCATTCTCATCACAAAAATCATTTTCCAACAAGCAGAGATAACTCTTCTACTTGGCCATCAACTGCATCTACACAAGAAAAGTGTTTTGAGCCAAACATGTCTTCATATTCTTCTTCAATTAGATGGGGGAATAACCACATGGAGTCTCTTGATTCTGAACAAGGGCTAGCCAATGCAAGGGAACAAATTTTCAAAGGTTATGGCTGTAATGATTATGTTGAAACAAGTTTGTCTATGGGACAAAATAATTCAATGTTTGGCCAAAAATTGCAAACTGATGATGATTCATGTGGTGGGGACAAAGACATAAGTTATAAAAGACCAAAGACAGCTTCCATTTCTTCTTCACTGCCTGTCTTGCTGAAGCCATGTTTGAATGATAGATGTTTAACTTTTCAGTCTGCAGAGTTTGTGGTTGGAGTTAAGCCTGGAATAGAAGACTTGGATCTTGAACTCAGGCTTGGGAAACTAACACAGAAAGTTTAG

Protein sequence

>MsaT014932.1

MEESLNMMWMKRKQILHSHNEGVPSFGTINNKSWEEKAFAEDAARILGGCIWPPRSYSCHFCKREFRSAQALGGHMNVHRRDRAKLKQSLSNHNELELHSHHKNHFPTSRDNSSTWPSTASTQEKCFEPNMSSYSSSIRWGNNHMESLDSEQGLANAREQIFKGYGCNDYVETSLSMGQNNSMFGQKLQTDDDSCGGDKDISYKRPKTASISSSLPVLLKPCLNDRCLTFQSAEFVVGVKPGIEDLDLELRLGKLTQKV*