AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015005


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015005 MtrunA17_Chr3g0113121 91.667 216 18 0 1 216 1 216 1.79e-139 389
MsaG015005 MtrunA17_Chr5g0436501 41.401 157 78 6 21 171 48 196 9.67e-21 87.4
MsaG015005 MtrunA17_Chr5g0429521 37.209 129 68 2 49 171 80 201 1.88e-17 79.0
MsaG015005 MtrunA17_Chr5g0440731 57.143 77 27 4 92 167 102 173 4.25e-17 77.8
MsaG015005 MtrunA17_Chr3g0104071 35.583 163 85 4 20 171 63 216 1.01e-16 77.4
MsaG015005 MtrunA17_Chr5g0409841 36.879 141 78 4 38 174 32 165 1.22e-16 75.9
MsaG015005 MtrunA17_Chr7g0241761 40.708 113 53 3 59 171 111 209 6.60e-16 74.7
MsaG015005 MtrunA17_Chr5g0440741 58.904 73 24 4 96 167 101 168 6.68e-16 74.7
MsaG015005 MtrunA17_Chr5g0409831 43.023 86 43 2 61 140 37 122 9.62e-16 73.2
MsaG015005 MtrunA17_Chr3g0135671 38.136 118 57 2 49 166 57 158 4.23e-15 71.6
MsaG015005 MtrunA17_Chr1g0210471 32.639 144 79 3 27 170 67 192 9.48e-14 68.2
MsaG015005 MtrunA17_Chr4g0059701 33.333 117 66 2 24 140 25 129 1.21e-13 68.2
MsaG015005 MtrunA17_Chr1g0162081 45.570 79 36 2 92 170 119 190 1.04e-12 65.9
MsaG015005 MtrunA17_Chr2g0331031 39.759 83 43 1 86 168 100 175 1.57e-12 65.5
MsaG015005 MtrunA17_Chr6g0488471 51.724 58 26 1 83 140 87 142 2.76e-12 64.3
MsaG015005 MtrunA17_Chr4g0055931 36.607 112 52 2 59 170 71 163 3.45e-12 64.3
MsaG015005 MtrunA17_Chr4g0062741 44.304 79 37 2 92 170 135 206 4.21e-12 63.9
MsaG015005 MtrunA17_Chr5g0415191 47.368 57 30 0 84 140 91 147 5.53e-12 63.9
MsaG015005 MtrunA17_Chr5g0432921 45.833 96 43 5 64 158 85 172 6.71e-12 62.8
MsaG015005 MtrunA17_Chr8g0389241 44.000 75 34 1 92 166 102 168 1.04e-11 62.8
MsaG015005 MtrunA17_Chr8g0365011 35.294 153 65 7 32 170 42 174 1.98e-11 62.0
MsaG015005 MtrunA17_Chr6g0452791 56.522 46 20 0 95 140 80 125 9.84e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015005 AT5G05790.2 42.188 128 62 3 49 172 82 201 1.54e-20 87.4
MsaG015005 AT5G05790.1 42.188 128 62 3 49 172 82 201 1.54e-20 87.4
MsaG015005 AT3G11280.2 38.854 157 78 7 21 172 54 197 1.16e-19 84.7
MsaG015005 AT3G11280.1 38.854 157 78 7 21 172 54 197 1.16e-19 84.7
MsaG015005 AT5G01200.1 45.299 117 50 4 56 171 112 215 2.17e-18 81.6
MsaG015005 AT2G38090.1 37.069 116 63 2 59 171 102 210 1.94e-16 76.6
MsaG015005 AT5G58900.1 44.681 94 45 1 78 171 124 210 4.86e-16 75.5
MsaG015005 AT5G08520.1 36.207 174 84 9 12 169 23 185 9.23e-16 74.7
MsaG015005 AT4G09450.1 39.837 123 61 4 70 186 66 181 3.47e-14 68.9
MsaG015005 AT5G04760.1 49.367 79 33 2 92 170 96 167 1.46e-13 67.4
MsaG015005 AT1G19000.2 50.877 57 28 0 84 140 91 147 5.00e-13 67.0
MsaG015005 AT1G19000.1 50.877 57 28 0 84 140 91 147 5.00e-13 67.0
MsaG015005 AT1G70000.1 41.096 73 41 1 70 140 71 143 1.62e-12 65.1
MsaG015005 AT1G70000.2 41.096 73 41 1 70 140 71 143 1.62e-12 65.1
MsaG015005 AT5G47390.1 44.444 81 38 1 86 166 87 160 1.83e-12 65.9
MsaG015005 AT3G10580.2 46.835 79 33 1 92 170 92 161 2.23e-12 64.7
MsaG015005 AT3G10580.1 46.835 79 33 1 92 170 92 161 2.37e-12 65.1
MsaG015005 AT1G49010.1 45.570 79 36 2 92 170 131 202 3.23e-12 64.7
MsaG015005 AT5G56840.1 52.632 57 27 0 92 148 88 144 1.87e-11 62.0
MsaG015005 AT5G61620.1 45.570 79 36 1 92 170 105 176 3.86e-11 61.6
MsaG015005 AT1G74840.2 55.102 49 22 0 92 140 93 141 6.15e-11 60.5
MsaG015005 AT1G74840.1 55.102 49 22 0 92 140 93 141 6.50e-11 60.8
MsaG015005 AT3G16350.1 48.148 54 28 0 87 140 129 182 8.78e-11 60.8

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATCTTGATCACATTCT+TGG 0.190525 3:-81446769 None:intergenic
TCAACCAATAATCATCTTCT+TGG 0.216049 3:-81446106 None:intergenic
ATTTCCAAGAAGATGATTAT+TGG 0.238910 3:+81446102 MsaT015005.1:CDS
GACTCATATGCGTGGTTATT+TGG 0.243758 3:+81446686 MsaT015005.1:CDS
ATAACTACTCTTGATCTTCT+TGG 0.251399 3:+81446656 MsaT015005.1:CDS
AGTTCTATTGTTATTATTAT+CGG 0.281602 3:-81446273 None:intergenic
TTTGAGCATGGCTTGCTACT+TGG 0.356659 3:-81446553 None:intergenic
AAAATATCAAAAGGAAACTT+AGG 0.373972 3:+81446618 MsaT015005.1:CDS
CATTCAAGAAAGTGGTTCAT+TGG 0.387227 3:+81446324 MsaT015005.1:CDS
AGAATGGTAGAGAATATTCT+AGG 0.388611 3:+81446053 None:intergenic
TTGAGCATGGCTTGCTACTT+GGG 0.417939 3:-81446552 None:intergenic
TATTTGTAGGCGTTTCTTGA+AGG 0.450413 3:+81446460 MsaT015005.1:intron
AAATATCAAAAGGAAACTTA+GGG 0.461487 3:+81446619 MsaT015005.1:CDS
AGATGATTATTGGTTGATGA+AGG 0.467158 3:+81446112 MsaT015005.1:CDS
AAAGTGGAAAGAAATCTCTA+AGG 0.472931 3:+81446505 MsaT015005.1:CDS
AAAGAAATCATTCAAGAAAG+TGG 0.476326 3:+81446316 MsaT015005.1:CDS
ATTTGTAGGCGTTTCTTGAA+GGG 0.494586 3:+81446461 MsaT015005.1:intron
GATCAAGAGTAGTTATATCA+TGG 0.499404 3:-81446649 None:intergenic
TACACGGCAAAGACGGAAAG+TGG 0.504210 3:+81446489 MsaT015005.1:CDS
GCATGGCTTGCTACTTGGGA+TGG 0.505418 3:-81446548 None:intergenic
TCTTGTCGTCTTCAATCACT+TGG 0.511899 3:-81446712 None:intergenic
GGACTTCTACACGGCAAAGA+CGG 0.559413 3:+81446482 MsaT015005.1:CDS
ATACATATGAACATATCAAG+TGG 0.590413 3:-81446804 None:intergenic
TTCTTGAAGGGACTTCTACA+CGG 0.594579 3:+81446473 MsaT015005.1:CDS
GAAGACGACAAGAATAACAA+TGG 0.599172 3:+81446722 MsaT015005.1:CDS
TCAGCACAACATCGATGAAG+TGG 0.609150 3:+81446166 MsaT015005.1:CDS
TTATTATTATCGGTTGCATG+AGG 0.632701 3:-81446263 None:intergenic
ATGCTCAAAAGTACGAACAA+AGG 0.658529 3:+81446567 MsaT015005.1:CDS
GCACAACATCGATGAAGTGG+TGG 0.668571 3:+81446169 MsaT015005.1:CDS
AATATCAAAAGGAAACTTAG+GGG 0.670754 3:+81446620 MsaT015005.1:CDS
GAAGTGATGACTCATATGCG+TGG 0.764866 3:+81446678 MsaT015005.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 81446056 81446811 81446056 ID=MsaG015005
Chr3 mRNA 81446056 81446811 81446056 ID=MsaT015005.1;Parent=MsaG015005
Chr3 exon 81446056 81446363 81446056 ID=MsaT015005.1.exon1;Parent=MsaT015005.1
Chr3 CDS 81446056 81446363 81446056 ID=cds.MsaT015005.1;Parent=MsaT015005.1
Chr3 exon 81446469 81446811 81446469 ID=MsaT015005.1.exon2;Parent=MsaT015005.1
Chr3 CDS 81446469 81446811 81446469 ID=cds.MsaT015005.1;Parent=MsaT015005.1
Gene Sequence

>MsaT015005.1

ATGGTAGAGAATATTCTAGGTTACAGTCTTCATAGCATGATGAATAATTTCCAAGAAGATGATTATTGGTTGATGAAGGAAACTATGCTATGTAGTGTTGATCAAATCAATCAGCACAACATCGATGAAGTGGTGGTAGAAAATAATATTGCATCGACGAGCGTTTCACTTTTTGGATATGATTCAGTTGATTCTTTCAACTTCTCTCCTCATGCAACCGATAATAATAACAATAGAACTACGAATATCGTAGAATCCAAAAAGAAATCATTCAAGAAAGTGGTTCATTGGTCACGTGATGAACACATGCGTTTCTTGAAGGGACTTCTACACGGCAAAGACGGAAAGTGGAAAGAAATCTCTAAGGACTATGTAAAAACAAAGACTCCATCCCAAGTAGCAAGCCATGCTCAAAAGTACGAACAAAGGCAAAAGCAAAGACTTGATGATGATAGCAAAAATATCAAAAGGAAACTTAGGGGTAGCATCCATGATATAACTACTCTTGATCTTCTTGGAAGTGATGACTCATATGCGTGGTTATTTGGTGACCAAGTGATTGAAGACGACAAGAATAACAATGGATCACTCTCAGTCACAAATTTCAACCAAGAATGTGATCAAGATCATGAAGTTGTTGCTTCCACTTGA

Protein sequence

>MsaT015005.1

MVENILGYSLHSMMNNFQEDDYWLMKETMLCSVDQINQHNIDEVVVENNIASTSVSLFGYDSVDSFNFSPHATDNNNNRTTNIVESKKKSFKKVVHWSRDEHMRFLKGLLHGKDGKWKEISKDYVKTKTPSQVASHAQKYEQRQKQRLDDDSKNIKRKLRGSIHDITTLDLLGSDDSYAWLFGDQVIEDDKNNNGSLSVTNFNQECDQDHEVVAST*