AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015091

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015091 MtrunA17_Chr3g0114161 84.683 1247 65 6 21 1164 25 1248 0.0 2077
MsaG015091 MtrunA17_Chr5g0437621 48.655 1338 470 29 5 1163 4 1303 0.0 1099
MsaG015091 MtrunA17_Chr7g0224051 67.644 853 158 11 412 1163 281 1116 0.0 1084
MsaG015091 MtrunA17_Chr7g0224051 72.696 293 73 2 47 338 1 287 1.79e-131 430
MsaG015091 MtrunA17_Chr1g0191141 62.073 878 207 14 387 1163 250 1102 0.0 990
MsaG015091 MtrunA17_Chr1g0191141 67.100 231 72 2 21 251 8 234 6.45e-88 308
MsaG015091 MtrunA17_Chr7g0233031 32.353 714 379 19 528 1150 532 1232 7.55e-86 304
MsaG015091 MtrunA17_Chr7g0225791 32.107 707 378 18 528 1144 574 1268 3.32e-82 294
MsaG015091 MtrunA17_Chr4g0047081 32.286 700 361 17 549 1154 548 1228 4.04e-82 293
MsaG015091 MtrunA17_Chr7g0233021 33.333 708 329 21 549 1144 537 1213 6.25e-81 289
MsaG015091 MtrunA17_Chr7g0232971 29.907 749 386 21 525 1164 580 1298 9.23e-79 283
MsaG015091 MtrunA17_Chr7g0224031 63.014 219 48 2 796 982 25 242 1.10e-78 258
MsaG015091 MtrunA17_Chr7g0232991 30.464 604 288 16 669 1154 61 650 1.39e-65 235
MsaG015091 MtrunA17_Chr2g0296421 28.678 673 339 20 530 1119 387 1001 1.30e-61 229
MsaG015091 MtrunA17_Chr8g0352361 29.942 688 356 27 507 1119 216 852 2.15e-59 221
MsaG015091 MtrunA17_Chr7g0233001 31.507 511 263 11 731 1154 3 513 2.01e-53 196
MsaG015091 MtrunA17_Chr7g0224021 73.267 101 25 1 650 750 1 99 6.59e-44 155
MsaG015091 MtrunA17_Chr2g0320781 48.214 112 58 0 1001 1112 129 240 1.79e-27 112
MsaG015091 MtrunA17_Chr5g0403921 24.129 373 226 7 825 1147 312 677 4.41e-19 93.2
MsaG015091 MtrunA17_Chr1g0190391 29.358 218 138 7 674 879 576 789 9.51e-19 92.4
MsaG015091 MtrunA17_Chr1g0190381 32.778 180 117 2 946 1124 179 355 1.32e-18 90.9
MsaG015091 MtrunA17_Chr4g0052341 24.932 365 212 13 824 1138 881 1233 1.70e-18 92.0
MsaG015091 MtrunA17_Chr8g0384531 39.216 102 61 1 1010 1111 780 880 5.06e-16 83.6
MsaG015091 MtrunA17_Chr5g0393461 32.639 144 93 2 1016 1157 585 726 1.66e-15 82.0
MsaG015091 MtrunA17_Chr4g0066741 23.094 446 289 10 715 1121 210 640 4.35e-14 77.4
MsaG015091 MtrunA17_Chr4g0040301 34.266 143 80 3 998 1133 414 549 5.25e-14 77.0
MsaG015091 MtrunA17_Chr3g0114111 88.889 36 4 0 1117 1152 1 36 9.54e-14 67.4
MsaG015091 MtrunA17_Chr2g0286291 24.330 448 281 14 715 1121 213 643 2.28e-13 75.1
MsaG015091 MtrunA17_Chr1g0183681 23.563 348 203 12 823 1119 548 883 1.98e-12 72.0
MsaG015091 MtrunA17_Chr3g0137091 33.775 151 79 4 1016 1153 657 799 1.84e-11 68.9
MsaG015091 MtrunA17_Chr1g0168031 35.514 107 68 1 996 1102 1013 1118 3.59e-11 67.8
MsaG015091 MtrunA17_Chr2g0320751 37.383 107 66 1 996 1102 876 981 4.91e-11 67.4
MsaG015091 MtrunA17_Chr7g0255821 38.298 94 57 1 1010 1102 690 783 5.26e-11 67.0
MsaG015091 MtrunA17_Chr5g0394201 33.333 135 83 1 1012 1146 714 841 8.09e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015091 AT5G20420.1 52.157 788 253 15 487 1163 486 1260 0.0 734
MsaG015091 AT5G20420.1 39.521 334 157 12 5 321 3 308 5.50e-56 213
MsaG015091 AT3G42670.2 49.706 849 272 17 442 1163 434 1254 0.0 729
MsaG015091 AT3G42670.2 38.667 300 163 7 5 302 3 283 1.02e-51 199
MsaG015091 AT3G42670.3 49.706 849 272 17 442 1163 434 1254 0.0 729
MsaG015091 AT3G42670.3 38.667 300 163 7 5 302 3 283 1.02e-51 199
MsaG015091 AT3G42670.1 49.706 849 272 17 442 1163 434 1254 0.0 729
MsaG015091 AT3G42670.1 38.667 300 163 7 5 302 3 283 1.02e-51 199
MsaG015091 AT3G42670.4 49.589 851 272 17 442 1163 434 1256 0.0 728
MsaG015091 AT3G42670.4 38.667 300 163 7 5 302 3 283 1.04e-51 199
MsaG015091 AT3G24340.1 32.078 717 382 20 509 1152 430 1114 2.61e-82 293
MsaG015091 AT1G05490.1 30.447 716 360 21 547 1148 693 1384 4.44e-71 261
MsaG015091 AT2G16390.1 28.916 415 234 14 478 879 177 543 1.78e-33 140
MsaG015091 AT2G16390.1 47.899 119 59 2 1001 1119 732 847 4.84e-22 103
MsaG015091 AT2G16390.2 28.916 415 234 14 478 879 180 546 1.99e-33 140
MsaG015091 AT2G16390.2 47.899 119 59 2 1001 1119 735 850 4.27e-22 103
MsaG015091 AT2G21450.1 30.723 332 180 10 583 896 183 482 1.31e-32 137
MsaG015091 AT2G21450.1 46.018 113 60 1 1007 1119 662 773 1.23e-21 102
MsaG015091 AT2G21450.3 30.723 332 180 10 583 896 92 391 1.34e-32 136
MsaG015091 AT2G21450.3 46.018 113 60 1 1007 1119 571 682 1.33e-21 101
MsaG015091 AT2G21450.2 30.723 332 180 10 583 896 190 489 1.80e-32 137
MsaG015091 AT2G21450.2 46.018 113 60 1 1007 1119 669 780 1.17e-21 102
MsaG015091 AT1G08600.4 30.047 213 133 6 679 879 718 926 3.90e-19 94.7
MsaG015091 AT1G08600.4 37.903 124 76 1 1002 1124 1159 1282 4.26e-17 87.8
MsaG015091 AT1G08600.3 30.047 213 133 6 679 879 718 926 3.90e-19 94.7
MsaG015091 AT1G08600.3 37.903 124 76 1 1002 1124 1159 1282 4.26e-17 87.8
MsaG015091 AT1G08600.2 30.047 213 133 6 679 879 718 926 3.90e-19 94.7
MsaG015091 AT1G08600.2 37.903 124 76 1 1002 1124 1159 1282 4.26e-17 87.8
MsaG015091 AT1G08600.1 37.903 124 76 1 1002 1124 1138 1261 4.42e-17 87.8
MsaG015091 AT3G19210.1 35.156 128 81 1 1016 1143 581 706 6.58e-16 83.6
MsaG015091 AT3G19210.2 35.156 128 81 1 1016 1143 579 704 7.47e-16 83.6
MsaG015091 AT3G19210.3 35.156 128 81 1 1016 1143 581 706 1.04e-15 82.8
MsaG015091 AT2G25170.3 27.848 316 183 12 825 1102 445 753 8.79e-15 80.1
MsaG015091 AT2G25170.1 27.848 316 183 12 825 1102 419 727 8.81e-15 80.1
MsaG015091 AT2G25170.4 27.848 316 183 12 825 1102 419 727 8.81e-15 80.1
MsaG015091 AT2G25170.2 27.848 316 183 12 825 1102 436 744 8.92e-15 80.1
MsaG015091 AT1G03750.1 33.566 143 81 3 998 1133 562 697 1.50e-13 75.9
MsaG015091 AT5G66750.1 34.228 149 96 2 1008 1155 562 709 1.59e-13 75.9
MsaG015091 AT5G63950.2 35.398 113 72 1 999 1111 744 855 4.66e-13 74.3
MsaG015091 AT5G63950.1 35.398 113 72 1 999 1111 761 872 4.73e-13 74.3
MsaG015091 AT3G32330.1 25.652 230 124 6 680 909 23 205 1.67e-12 70.5
MsaG015091 AT2G18760.3 38.542 96 58 1 1024 1119 840 934 4.39e-12 71.2
MsaG015091 AT2G18760.4 38.542 96 58 1 1024 1119 781 875 4.70e-12 71.2
MsaG015091 AT2G18760.1 38.542 96 58 1 1024 1119 781 875 4.70e-12 71.2
MsaG015091 AT2G18760.2 38.542 96 58 1 1024 1119 781 875 4.70e-12 71.2
MsaG015091 AT2G18760.7 38.542 96 58 1 1024 1119 781 875 4.70e-12 71.2
MsaG015091 AT2G18760.5 38.542 96 58 1 1024 1119 781 875 4.70e-12 71.2
MsaG015091 AT2G18760.6 38.542 96 58 1 1024 1119 781 875 4.70e-12 71.2
MsaG015091 AT2G28290.2 41.935 93 54 0 1010 1102 1113 1205 5.49e-11 68.2
MsaG015091 AT2G28290.3 41.935 93 54 0 1010 1102 1113 1205 5.58e-11 68.2
MsaG015091 AT2G28290.4 41.935 93 54 0 1010 1102 1113 1205 5.58e-11 68.2
MsaG015091 AT2G28290.6 41.935 93 54 0 1010 1102 1114 1206 5.58e-11 68.2
MsaG015091 AT2G28290.1 41.935 93 54 0 1010 1102 1113 1205 6.18e-11 67.8
MsaG015091 AT2G28290.5 41.935 93 54 0 1010 1102 1114 1206 6.18e-11 67.8

Find 169 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTGTTTCAGAACAATTT+TGG 0.187519 3:-82604507 MsaT015091.1:CDS
ATTAGTCAAATAATGATATT+TGG 0.190711 3:+82606655 None:intergenic
TGACGGTGATGGCAAGTTTA+AGG 0.211148 3:-82606702 MsaT015091.1:CDS
CCTCTGAAATTAAGCCTTAT+AGG 0.223041 3:-82607270 MsaT015091.1:CDS
TATAGATTGGATGAAGAAAT+TGG 0.225183 3:-82605742 MsaT015091.1:CDS
AACAAGCATGTTGGAGAAAA+AGG 0.240380 3:-82606231 MsaT015091.1:CDS
TCATCACTTGATATTGTTGT+AGG 0.263435 3:+82606572 None:intergenic
AACCTTGATTTAGTGCTTCT+AGG 0.271299 3:+82604582 None:intergenic
ACTATCCTTGGATTCTTTCT+TGG 0.272245 3:+82605777 None:intergenic
CATATAAGTTGTTCTCTTGT+AGG 0.274870 3:+82606838 None:intergenic
TTATAATCTTCATCAACTTT+TGG 0.276698 3:+82605253 None:intergenic
GCATAACATTGCTGGCTCTA+TGG 0.281856 3:-82605095 MsaT015091.1:CDS
CCACGTGTAGAGTGTGGTTT+TGG 0.284196 3:+82604926 None:intergenic
ACTATCTATTATTTCCTTGC+AGG 0.289125 3:+82606319 None:intergenic
GCAAAATGGCAAAGAGATTA+TGG 0.292136 3:-82603847 MsaT015091.1:CDS
ACATCAAGTTATCTTCTTGA+TGG 0.297384 3:-82606122 MsaT015091.1:CDS
CAGAAGAGGATGGTGGTAAG+AGG 0.300780 3:-82606790 MsaT015091.1:CDS
AGAACTTCATGAACAAATTT+CGG 0.305114 3:+82604453 None:intergenic
CTTACTGGAATAGACGTAAA+TGG 0.306756 3:+82608018 None:intergenic
ATTCAATGGCGCCCATAAAA+AGG 0.307589 3:-82609176 None:intergenic
TCGACCGGGCCAGCAGAAAA+TGG 0.318508 3:-82603625 MsaT015091.1:CDS
GGAAGAAAATCCTGATGATT+TGG 0.323484 3:-82606177 MsaT015091.1:CDS
TTCTCTGCTTGCCATTTAGA+AGG 0.325243 3:+82603489 None:intergenic
CAAGATTGTGTATCAAGTTA+TGG 0.325678 3:-82607113 MsaT015091.1:CDS
TGGTGCTGATGTGTTGAATT+TGG 0.328233 3:-82606609 MsaT015091.1:CDS
ATTGATATATCTATGCTATT+AGG 0.336391 3:-82608066 MsaT015091.1:CDS
ACTTCATGTTGTGGATGATA+TGG 0.339055 3:-82606480 MsaT015091.1:CDS
TCTGATGAATCCCACCTATA+AGG 0.345661 3:+82607256 None:intergenic
CTCAACCTCAAGAGGGTAAC+TGG 0.345817 3:+82604121 None:intergenic
AGGGCTTTCTCTCAATGCTT+TGG 0.345836 3:+82604635 None:intergenic
AGCAAATTTGATTTGAGAAA+AGG 0.352698 3:-82603996 MsaT015091.1:CDS
GCACTTTGTCATCAGATATT+CGG 0.354010 3:-82608143 MsaT015091.1:CDS
TCAACTTCATGTGATGGCTT+TGG 0.355507 3:+82606956 None:intergenic
TTAATGAAATCGACAACGTT+TGG 0.355745 3:-82605183 MsaT015091.1:CDS
AAGTATTATGTTGAAGCCTT+TGG 0.358364 3:+82607320 None:intergenic
ACAGAAACTTCAGAAGAAAA+TGG 0.360997 3:-82604153 MsaT015091.1:intron
TCATTAATCAATGGTTCATC+AGG 0.361796 3:+82605199 None:intergenic
TTGTGGAAGATCCTTCTAAA+TGG 0.362063 3:-82603500 MsaT015091.1:CDS
GATAGCTGGAATGCCCAAAA+AGG 0.366087 3:-82605327 MsaT015091.1:intron
TTTCTTTGGCATAACATTGC+TGG 0.370580 3:-82605103 MsaT015091.1:CDS
GAAATGTATCCCTTCCCATT+TGG 0.379249 3:+82606448 None:intergenic
ACAGTGCAGTGAAGAAAATA+AGG 0.381838 3:-82605290 MsaT015091.1:CDS
AAGATTGTGTATCAAGTTAT+GGG 0.381846 3:-82607112 MsaT015091.1:CDS
TCATCAAGTATCAAGATTCC+AGG 0.400291 3:+82604615 None:intergenic
TAATGAAATCGACAACGTTT+GGG 0.400568 3:-82605182 MsaT015091.1:CDS
AAAGAGATTATGGTACTAAC+AGG 0.411733 3:-82603837 MsaT015091.1:CDS
CACAATCTAGATTACTCGTT+GGG 0.425426 3:-82607216 MsaT015091.1:CDS
AGCATAGATATATCAATTCC+AGG 0.429264 3:+82608072 None:intergenic
GCATTTGAAGCAGTATTGAA+TGG 0.430979 3:-82608246 MsaT015091.1:CDS
ATCATGTTCACAACTATCCT+TGG 0.438359 3:+82605765 None:intergenic
AGGACCAGAGGTCGTTTCCT+TGG 0.438685 3:+82604953 None:intergenic
ATATAAGTTGTTCTCTTGTA+GGG 0.439124 3:+82606839 None:intergenic
ATTTCTTGTGCAAAGAAGAA+TGG 0.444673 3:-82606629 MsaT015091.1:CDS
TTGTCGATTTCATTAATCAA+TGG 0.444704 3:+82605190 None:intergenic
TGATGATTTGGATGCCTTGT+GGG 0.445174 3:-82606165 MsaT015091.1:CDS
ATACTGATATTATTAGTCCT+TGG 0.448089 3:-82607084 MsaT015091.1:CDS
TCAAGTGATGAAGAAGTTGA+TGG 0.452513 3:-82606560 MsaT015091.1:CDS
CACAAGGCATCCAAATCATC+AGG 0.457690 3:+82606167 None:intergenic
AGTAATATTCTCAATTGCAT+TGG 0.461042 3:+82604537 None:intergenic
AAGAAAAGAATTGATGAGAA+AGG 0.461369 3:+82604997 None:intergenic
GTTAACTTATATGATGATCA+AGG 0.462568 3:-82607415 MsaT015091.1:CDS
CATGATTATGAAGAATGAAA+AGG 0.465758 3:-82603409 MsaT015091.1:CDS
ATCAAAAGCTCAACCTCAAG+AGG 0.468504 3:+82604113 None:intergenic
CATGAAGAACTAGATCATAA+AGG 0.470683 3:-82606365 MsaT015091.1:CDS
ACATCAAATTGAGAAACAAT+AGG 0.472158 3:+82607016 None:intergenic
ATATCACATGCTCCTGGAGC+AGG 0.473334 3:-82605025 MsaT015091.1:CDS
TCTTTAAAGAGAAGATAAAG+AGG 0.473356 3:-82606508 MsaT015091.1:CDS
GATTGAAGATGATATACTTA+GGG 0.473462 3:-82603466 MsaT015091.1:CDS
GTGTATCAGTTATTGACGAC+CGG 0.474170 3:-82603597 MsaT015091.1:CDS
AGATTGAAGATGATATACTT+AGG 0.475140 3:-82603467 MsaT015091.1:CDS
ACACAACCTACGTAACGTTC+AGG 0.477670 3:+82606899 None:intergenic
ACACCCTAGTGTTCTTGTCA+TGG 0.484111 3:-82604724 MsaT015091.1:CDS
ATTTCCAAGGAAACGACCTC+TGG 0.484818 3:-82604957 MsaT015091.1:CDS
TGGTGGTAAGAGGGATCAAA+AGG 0.487310 3:-82606780 MsaT015091.1:CDS
CTGGGGAGGTTCGACGGCCA+TGG 0.487953 3:+82604856 None:intergenic
CACCCTAGTGTTCTTGTCAT+GGG 0.490664 3:-82604723 MsaT015091.1:CDS
GTGCCGGTTTATCTTATCCA+TGG 0.492001 3:-82604873 MsaT015091.1:CDS
TTACGATAATTAGCATCAAA+TGG 0.492300 3:+82609132 None:intergenic
ACAGTAGGCTCTTCTTCAGT+TGG 0.493427 3:+82606272 None:intergenic
GAGCTAGACTTGTTTGAAAG+AGG 0.495712 3:-82603813 MsaT015091.1:CDS
TATTATGTTGAAGCCTTTGG+AGG 0.500254 3:+82607323 None:intergenic
GTTACTTGAAGTTATTTCCA+AGG 0.501704 3:-82604970 MsaT015091.1:CDS
AAGCAGTATTGAATGGATCA+TGG 0.507736 3:-82608239 MsaT015091.1:CDS
AAAATGGAAGATTCCTGTGC+CGG 0.511195 3:-82604889 MsaT015091.1:CDS
TATTGATGAGGAGAAGATGC+AGG 0.512826 3:-82604307 MsaT015091.1:CDS
AAGCCATCACATGAAGTTGA+AGG 0.513509 3:-82606953 MsaT015091.1:CDS
CAGATATTCGGATAAGATCA+AGG 0.513601 3:-82608131 MsaT015091.1:CDS
TTCAGTATCAATGAAACGTA+AGG 0.514290 3:+82608176 None:intergenic
ACTTCATGTGATGGCTTTGG+TGG 0.514522 3:+82606959 None:intergenic
CTTCAACATAATACTTGTGA+AGG 0.515406 3:-82607313 MsaT015091.1:CDS
TCAATCAATGCTTGTGCTGA+AGG 0.515842 3:-82603735 MsaT015091.1:CDS
AACACAGCCACCACTTGAGT+TGG 0.516402 3:+82605048 None:intergenic
GAAGAACTACTTGGAAAGAG+TGG 0.517977 3:-82603551 MsaT015091.1:CDS
AGGTTGTGTTGTTGAGAAGT+TGG 0.518892 3:-82606885 MsaT015091.1:CDS
CGGCCATGGATAAGATAAAC+CGG 0.521874 3:+82604870 None:intergenic
TTCACAACATTTATAAGCCA+AGG 0.522728 3:+82607067 None:intergenic
GTTATTGACGACCGGGTCGA+TGG 0.524989 3:-82603589 MsaT015091.1:CDS
CATGGCCGTCGAACCTCCCC+AGG 0.526398 3:-82604855 MsaT015091.1:intron
CCATGGATGTATTGATCCGA+GGG 0.527482 3:+82604088 None:intergenic
AAGCCTTCAACTTCATGTGA+TGG 0.530172 3:+82606950 None:intergenic
TTGTGAACATGATTATAGAT+TGG 0.534056 3:-82605755 MsaT015091.1:CDS
TTCCACTTGTCATTTACAGT+AGG 0.536954 3:+82606257 None:intergenic
AGATGATATACTTAGGGAGA+TGG 0.537029 3:-82603460 MsaT015091.1:CDS
GTTGTTGAGAAGTTGGAAGT+TGG 0.537957 3:-82606878 MsaT015091.1:CDS
ATCCTAGAAGCACTAAATCA+AGG 0.543534 3:-82604584 MsaT015091.1:CDS
AGCTAGACTTGTTTGAAAGA+GGG 0.545005 3:-82603812 MsaT015091.1:CDS
TGTGTTATATCACATGCTCC+TGG 0.545385 3:-82605031 MsaT015091.1:CDS
AAATTGACATGAACATTGAT+CGG 0.547067 3:+82607441 None:intergenic
GGAATCTTGATACTTGATGA+AGG 0.550715 3:-82604612 MsaT015091.1:CDS
CTCTATGGAACCATCACTCA+TGG 0.552151 3:-82605080 MsaT015091.1:CDS
TTGAACCTGGGGAGGTTCGA+CGG 0.553335 3:+82604850 None:intergenic
TGGATAAGATAAACCGGCAC+AGG 0.556571 3:+82604876 None:intergenic
AGCCTACTGTAAATGACAAG+TGG 0.559328 3:-82606259 MsaT015091.1:CDS
ATAAGTATAGAAGAACTACT+TGG 0.563508 3:-82603560 MsaT015091.1:CDS
ACCATGGATGTATTGATCCG+AGG 0.564240 3:+82604087 None:intergenic
CCCTCGGATCAATACATCCA+TGG 0.564663 3:-82604088 MsaT015091.1:CDS
AAGAACTACTTGGAAAGAGT+GGG 0.567137 3:-82603550 MsaT015091.1:CDS
ATTCAATGCTGATGCCTGCA+AGG 0.569248 3:-82606333 MsaT015091.1:CDS
TATGATGATCAAGGTTCACT+TGG 0.572239 3:-82607406 MsaT015091.1:CDS
AGAAGAGGATGGTGGTAAGA+GGG 0.572455 3:-82606789 MsaT015091.1:CDS
TGAAATTAAGCCTTATAGGT+GGG 0.572501 3:-82607266 MsaT015091.1:CDS
CCTATAAGGCTTAATTTCAG+AGG 0.573942 3:+82607270 None:intergenic
GATTCTAAATACAGAAGGAA+AGG 0.574058 3:-82604420 MsaT015091.1:CDS
GTGGAAGGAAACAAGCATGT+TGG 0.575605 3:-82606240 MsaT015091.1:CDS
CATCAAGTATCAAGATTCCA+GGG 0.577717 3:+82604616 None:intergenic
GAGTACCAGTTACCCTCTTG+AGG 0.579454 3:-82604126 MsaT015091.1:CDS
TCACAATCTAGATTACTCGT+TGG 0.580048 3:-82607217 MsaT015091.1:CDS
ACTTAGGGAGATGGTTGAAG+AGG 0.582988 3:-82603451 MsaT015091.1:CDS
CGTCGAACTTGAACCTGGGG+AGG 0.583803 3:+82604842 None:intergenic
CAATTGAGAATATTACTCTC+CGG 0.585991 3:-82604531 MsaT015091.1:CDS
GCTTGCGACGTTCGAAACGT+AGG 0.587148 3:-82606931 MsaT015091.1:CDS
GAAGCACTAAATCAAGGTTG+AGG 0.587727 3:-82604578 MsaT015091.1:CDS
ATTGTTCTGAAACAATGTGC+CGG 0.589502 3:+82604512 None:intergenic
TGATACCAAGAAAGAATCCA+AGG 0.593538 3:-82605782 MsaT015091.1:CDS
ATTGACGACCGGGTCGATGG+AGG 0.593637 3:-82603586 MsaT015091.1:CDS
TAGCCCATGACAAGAACACT+AGG 0.596138 3:+82604720 None:intergenic
CTGAAATTAAGCCTTATAGG+TGG 0.602209 3:-82607267 MsaT015091.1:CDS
AGCATCGAAAAGCGATGACG+AGG 0.602778 3:-82606198 MsaT015091.1:CDS
GAAAGAACGTCATTTGCTTG+AGG 0.606173 3:-82604382 MsaT015091.1:CDS
AGTTAGATTCTAAATACAGA+AGG 0.607419 3:-82604425 MsaT015091.1:CDS
AAAGCATTGAGAGAAAGCCC+TGG 0.610887 3:-82604633 MsaT015091.1:CDS
ATTCGCACACAGAAAATACA+TGG 0.611483 3:-82604658 MsaT015091.1:CDS
CTGATGATTTGGATGCCTTG+TGG 0.612273 3:-82606166 MsaT015091.1:CDS
TACTGTAAATGACAAGTGGA+AGG 0.615462 3:-82606255 MsaT015091.1:CDS
CTTATCTTCCTCCATCGACC+CGG 0.615595 3:+82603578 None:intergenic
GGAATATACTGTCTTCGATG+TGG 0.617949 3:-82605721 MsaT015091.1:CDS
TGTATTCATTTCTTCCCACA+AGG 0.620617 3:+82606151 None:intergenic
GAGGAAGAGACTTCATGTTG+TGG 0.623646 3:-82606489 MsaT015091.1:CDS
AAACTCTTCAGATTCAGAAG+AGG 0.627550 3:-82606804 MsaT015091.1:CDS
TCTAAATACAGAAGGAAAGG+CGG 0.627794 3:-82604417 MsaT015091.1:CDS
TGTATCAGTTATTGACGACC+GGG 0.631867 3:-82603596 MsaT015091.1:CDS
TCTTCAGATTCAGAAGAGGA+TGG 0.632676 3:-82606800 MsaT015091.1:CDS
TCAAGGTTCACTTGGTACAG+AGG 0.634309 3:-82607398 MsaT015091.1:CDS
GATGTGTTGAATTTGGAGTA+CGG 0.641704 3:-82606602 MsaT015091.1:CDS
AATTAACTCAGATATTGATG+AGG 0.647649 3:-82604319 MsaT015091.1:CDS
AAATTTGAAGATCAAAGCAG+CGG 0.654526 3:-82603777 MsaT015091.1:CDS
ATTGATGAGGAGAAGATGCA+GGG 0.655538 3:-82604306 MsaT015091.1:CDS
GAATGGATCATGGCAACCTG+TGG 0.660516 3:-82608229 MsaT015091.1:CDS
CCAAAACCACACTCTACACG+TGG 0.662197 3:-82604926 MsaT015091.1:CDS
GTCTTGGCAAGATACAGAAG+TGG 0.663388 3:-82604752 MsaT015091.1:intron
TCAAAAGCTCAACCTCAAGA+GGG 0.663408 3:+82604114 None:intergenic
AGATTGTGTATCAAGTTATG+GGG 0.667010 3:-82607111 MsaT015091.1:CDS
TTATCTTCTTGATGGAACTG+CGG 0.671963 3:-82606114 MsaT015091.1:intron
TCAGATTCAGAAGAGGATGG+TGG 0.672715 3:-82606797 MsaT015091.1:CDS
AAGCTATTAATGTGAACACA+AGG 0.677127 3:-82606736 MsaT015091.1:CDS
GAACATTGATCGGATTTGTG+AGG 0.698234 3:+82607451 None:intergenic
AGCCCATGACAAGAACACTA+GGG 0.709112 3:+82604721 None:intergenic
GTACAGCCTGAACGTTACGT+AGG 0.720601 3:-82606905 MsaT015091.1:CDS
GAAGAACTAGATCATAAAGG+CGG 0.724609 3:-82606362 MsaT015091.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 82603395 82609193 82603395 ID=MsaG015091
Chr3 mRNA 82603395 82609193 82603395 ID=MsaT015091.1;Parent=MsaG015091
Chr3 exon 82603395 82603871 82603395 ID=MsaT015091.1.exon8;Parent=MsaT015091.1
Chr3 CDS 82603395 82603871 82603395 ID=cds.MsaT015091.1;Parent=MsaT015091.1
Chr3 exon 82603989 82604171 82603989 ID=MsaT015091.1.exon7;Parent=MsaT015091.1
Chr3 CDS 82603989 82604171 82603989 ID=cds.MsaT015091.1;Parent=MsaT015091.1
Chr3 exon 82604266 82604763 82604266 ID=MsaT015091.1.exon6;Parent=MsaT015091.1
Chr3 CDS 82604266 82604763 82604266 ID=cds.MsaT015091.1;Parent=MsaT015091.1
Chr3 exon 82604856 82605344 82604856 ID=MsaT015091.1.exon5;Parent=MsaT015091.1
Chr3 CDS 82604856 82605344 82604856 ID=cds.MsaT015091.1;Parent=MsaT015091.1
Chr3 exon 82605681 82605827 82605681 ID=MsaT015091.1.exon4;Parent=MsaT015091.1
Chr3 CDS 82605681 82605827 82605681 ID=cds.MsaT015091.1;Parent=MsaT015091.1
Chr3 exon 82606115 82607512 82606115 ID=MsaT015091.1.exon3;Parent=MsaT015091.1
Chr3 CDS 82606115 82607512 82606115 ID=cds.MsaT015091.1;Parent=MsaT015091.1
Chr3 exon 82608023 82608267 82608023 ID=MsaT015091.1.exon2;Parent=MsaT015091.1
Chr3 CDS 82608023 82608267 82608023 ID=cds.MsaT015091.1;Parent=MsaT015091.1
Chr3 exon 82609136 82609193 82609136 ID=MsaT015091.1.exon1;Parent=MsaT015091.1
Chr3 CDS 82609136 82609193 82609136 ID=cds.MsaT015091.1;Parent=MsaT015091.1
Gene Sequence

>MsaT015091.1

ATGGCGCCCATAAAAAGGCATTTGCATCAATGGAAGCATCCATTTGATGCTAATTATCCATTTGAAGCAGTATTGAATGGATCATGGCAACCTGTGGAACTCTTAAAAATAGAGTCAGGAAAAACTACCTTACGTTTCATTGATACTGAACAAATTCAAAGCACTTTGTCATCAGATATTCGGATAAGATCAAGGAAAGCTACTTCATCTGATTGCTCCAGCTTTTTACGACCTGGAATTGATATATCTATGCTATTAGGCTATCGATACAATGATAATTCAGATCCATTTACGTCTATTCCAATGTGGATTGATGCAAGAATAAATTCTATACATAGACAGCCTCACAAATCCGATCAATGTTCATGTCAATTTTATGTTAACTTATATGATGATCAAGGTTCACTTGGTACAGAGGTAAAAACTCTCAATAAAGAGGTTAATGCAATTGAAATAGATCAAATTTTCATCCTCCAAAGGCTTCAACATAATACTTGTGAAGGCTTGTTAAAAGAAAATGATACCTCTGAAATTAAGCCTTATAGGTGGGATTCATCAGAAGATTGCTCTTCATTATCACAATCTAGATTACTCGTTGGGAAATTTTCATCTGATCTTTCGTGGTTTGTAATTACATCTTTTTTAAAGAAGGTTTCATTCCATATAAGATCTGTGCAAAACAAGATTGTGTATCAAGTTATGGGGAATGATACTGATATTATTAGTCCTTGGCTTATAAATGTTGTGAACTTCAGCTTAAAAAAGGAATCACTAGTACCTATTGTTTCTCAATTTGATGTATTTTATGATTATGATGGAGAAGAAGATGAAGCGCCACCAAAGCCATCACATGAAGTTGAAGGCTTGCGACGTTCGAAACGTAGGAATGTACAGCCTGAACGTTACGTAGGTTGTGTTGTTGAGAAGTTGGAAGTTGGTAATTTTCGAACGTGTCCCTACAAGAGAACAACTTATATGATAGAAGATGAAAACTCTTCAGATTCAGAAGAGGATGGTGGTAAGAGGGATCAAAAGGTTAATAAGCTAAATCATTGCAAAGCTATTAATGTGAACACAAGGAACAAAAATTCTGACGGTGATGGCAAGTTTAAGGTACATAAAACTGATGACATTTCTTCCAAATATCATTATTTGACTAATTATATTTCTTGTGCAAAGAAGAATGGTGCTGATGTGTTGAATTTGGAGTACGGCAATGAGCCTACAACAATATCAAGTGATGAAGAAGTTGATGGTAATGTTACTTTGAGAAATTATAATAGTTTCTTTAAAGAGAAGATAAAGAGGAAGAGACTTCATGTTGTGGATGATATGGATTTTGAAACCAAATGGGAAGGGATACATTTCAAAAAAGAAGCTCCAACAGAAAATTTTCATTCGAGTTATTCGTCTAACAGAAGTCATGTTCATGAAGAACTAGATCATAAAGGCGGTAGAACATTCAATGCTGATGCCTGCAAGGAAATAATAGATAGTTACATGAAGAATTTTGATAGTCTACCAACTGAAGAAGAGCCTACTGTAAATGACAAGTGGAAGGAAACAAGCATGTTGGAGAAAAAGGAAGAAGAAAAAGCATCGAAAAGCGATGACGAGGAAGAAAATCCTGATGATTTGGATGCCTTGTGGGAAGAAATGAATACAGCTTTGACATCAAGTTATCTTCTTGATGGAACTGCGGGTTCAACTGCAGAGTTTGTAGCTGATACCAAGAAAGAATCCAAGGATAGTTGTGAACATGATTATAGATTGGATGAAGAAATTGGAATATACTGTCTTCGATGTGGATTTGTGAAAACTGCAATACGAGACATTTCTGAGCCATTTCTGGAATGCCCAAAAAGGTACAAAGAGGAGAAACAGTGCAGTGAAGAAAATAAGGAACATAAATCAGAGCCAAAAGTTGATGAAGATTATAATAATGATATGTTCTCGACTCATGCTACTGATCCTGATGAACCATTGATTAATGAAATCGACAACGTTTGGGAATTGATTCCTGAACTTGAAGAAAAGATGCATGAACACCAGAAAAAAGCTTTTGAATTTCTTTGGCATAACATTGCTGGCTCTATGGAACCATCACTCATGGAAGAAAAATCCAACTCAAGTGGTGGCTGTGTTATATCACATGCTCCTGGAGCAGGAAAAACCTTTCTCATCAATTCTTTTCTTGTCAGTTACTTGAAGTTATTTCCAAGGAAACGACCTCTGGTCCTAGCTCCAAAACCACACTCTACACGTGGCGAAAAGAGTTCAAAAAATGGAAGATTCCTGTGCCGGTTTATCTTATCCATGGCCGTCGAACCTCCCCAGATACAGAAGTGGCACTCACACCCTAGTGTTCTTGTCATGGGCTATACTTCATTTTTACAATTAATGAGATCAAAAGATACAAAATTCGCACACAGAAAATACATGGCCAAAGCATTGAGAGAAAGCCCTGGAATCTTGATACTTGATGAAGGACACAATCCTAGAAGCACTAAATCAAGGTTGAGGAAATGTTTAATGAAACTTCCAATGCAATTGAGAATATTACTCTCCGGCACATTGTTTCAGAACAATTTTGGTGAATATTTCAACACACTTTGTTTAGCTAGACCGAAATTTGTTCATGAAGTTCTACAAGAGTTAGATTCTAAATACAGAAGGAAAGGCGGAAAAGTTGCGAAGAAAGAACGTCATTTGCTTGAGGCGCGCGCAAGAAAATTCTTCTTGAACAACATTGAAAAGAAAATTAACTCAGATATTGATGAGGAGAAGATGCAGGGTCTATATGTGCTGCGAAAAATCACTTCGGGTTTTATTGATAAACTTCAGAAGAAAATGGCTGAGAGTACCAGTTACCCTCTTGAGGTTGAGCTTTTGATAACCCTCGGATCAATACATCCATGGTTGATCAAAACAGCTGCATCATGTGCAACTAAGTTTTTTGCGGAGGAAGAATTGAAGAAACTAGAAACAAGCAAATTTGATTTGAGAAAAGGTTCCAAATGGCAAAATGGCAAAGAGATTATGGTACTAACAGGTGAGCTAGACTTGTTTGAAAGAGGGAAAGTGATAGATAAATTTGAAGATCAAAGCAGCGGTTCAAAGATACTTCTTGCTTCAATCAATGCTTGTGCTGAAGGTATAAGTTTAACAGCAGCTTCTAGAGTGATTTTTCTGGACTCTGAGTGGAATCCTTCAAAAACAAAGCAAGCTATTGCACGCGCTTTTCGACCGGGCCAGCAGAAAATGGTTTATGTGTATCAGTTATTGACGACCGGGTCGATGGAGGAAGATAAGTATAGAAGAACTACTTGGAAAGAGTGGGTTTCTTGCATGATTTTTAGTGAGGAGTTTGTGGAAGATCCTTCTAAATGGCAAGCAGAGAAGATTGAAGATGATATACTTAGGGAGATGGTTGAAGAGGATAAGTCTAAAGCTATTCACATGATTATGAAGAATGAAAAGGCTTCAACAATCTAA
Protein sequence

>MsaT015091.1

MAPIKRHLHQWKHPFDANYPFEAVLNGSWQPVELLKIESGKTTLRFIDTEQIQSTLSSDIRIRSRKATSSDCSSFLRPGIDISMLLGYRYNDNSDPFTSIPMWIDARINSIHRQPHKSDQCSCQFYVNLYDDQGSLGTEVKTLNKEVNAIEIDQIFILQRLQHNTCEGLLKENDTSEIKPYRWDSSEDCSSLSQSRLLVGKFSSDLSWFVITSFLKKVSFHIRSVQNKIVYQVMGNDTDIISPWLINVVNFSLKKESLVPIVSQFDVFYDYDGEEDEAPPKPSHEVEGLRRSKRRNVQPERYVGCVVEKLEVGNFRTCPYKRTTYMIEDENSSDSEEDGGKRDQKVNKLNHCKAINVNTRNKNSDGDGKFKVHKTDDISSKYHYLTNYISCAKKNGADVLNLEYGNEPTTISSDEEVDGNVTLRNYNSFFKEKIKRKRLHVVDDMDFETKWEGIHFKKEAPTENFHSSYSSNRSHVHEELDHKGGRTFNADACKEIIDSYMKNFDSLPTEEEPTVNDKWKETSMLEKKEEEKASKSDDEEENPDDLDALWEEMNTALTSSYLLDGTAGSTAEFVADTKKESKDSCEHDYRLDEEIGIYCLRCGFVKTAIRDISEPFLECPKRYKEEKQCSEENKEHKSEPKVDEDYNNDMFSTHATDPDEPLINEIDNVWELIPELEEKMHEHQKKAFEFLWHNIAGSMEPSLMEEKSNSSGGCVISHAPGAGKTFLINSFLVSYLKLFPRKRPLVLAPKPHSTRGEKSSKNGRFLCRFILSMAVEPPQIQKWHSHPSVLVMGYTSFLQLMRSKDTKFAHRKYMAKALRESPGILILDEGHNPRSTKSRLRKCLMKLPMQLRILLSGTLFQNNFGEYFNTLCLARPKFVHEVLQELDSKYRRKGGKVAKKERHLLEARARKFFLNNIEKKINSDIDEEKMQGLYVLRKITSGFIDKLQKKMAESTSYPLEVELLITLGSIHPWLIKTAASCATKFFAEEELKKLETSKFDLRKGSKWQNGKEIMVLTGELDLFERGKVIDKFEDQSSGSKILLASINACAEGISLTAASRVIFLDSEWNPSKTKQAIARAFRPGQQKMVYVYQLLTTGSMEEDKYRRTTWKEWVSCMIFSEEFVEDPSKWQAEKIEDDILREMVEEDKSKAIHMIMKNEKASTI*