Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015111 | XP_003601024.1 | 93.640 | 283 | 8 | 1 | 1 | 283 | 1 | 273 | 0.0 | 546 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015111 | sp|Q9LYL9|Y3623_ARATH | 47.273 | 275 | 142 | 3 | 1 | 274 | 1 | 273 | 3.62e-83 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015111 | tr|G7J2Y2|G7J2Y2_MEDTR | 93.640 | 283 | 8 | 1 | 1 | 283 | 1 | 273 | 0.0 | 546 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG015111 | TR | TRAF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015111 | MtrunA17_Chr3g0114411 | 93.640 | 283 | 8 | 1 | 1 | 283 | 1 | 273 | 0.0 | 546 |
| MsaG015111 | MtrunA17_Chr3g0125321 | 27.317 | 205 | 140 | 4 | 69 | 268 | 36 | 236 | 2.06e-19 | 85.1 |
| MsaG015111 | MtrunA17_Chr5g0447111 | 32.484 | 157 | 102 | 3 | 87 | 243 | 142 | 294 | 1.23e-15 | 75.9 |
| MsaG015111 | MtrunA17_Chr3g0103031 | 32.143 | 168 | 106 | 4 | 76 | 243 | 134 | 293 | 5.16e-14 | 71.2 |
| MsaG015111 | MtrunA17_Chr5g0447191 | 33.333 | 126 | 81 | 2 | 100 | 225 | 16 | 138 | 1.90e-11 | 62.0 |
| MsaG015111 | MtrunA17_Chr1g0159831 | 33.103 | 145 | 92 | 3 | 97 | 241 | 154 | 293 | 4.04e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015111 | AT3G56230.1 | 47.273 | 275 | 142 | 3 | 1 | 274 | 1 | 273 | 3.68e-84 | 254 |
| MsaG015111 | AT1G01640.1 | 47.150 | 193 | 96 | 4 | 87 | 278 | 1 | 188 | 1.26e-54 | 176 |
| MsaG015111 | AT1G01640.2 | 47.674 | 172 | 84 | 4 | 108 | 278 | 1 | 167 | 2.37e-47 | 157 |
| MsaG015111 | AT2G40450.1 | 40.000 | 185 | 106 | 3 | 90 | 269 | 4 | 188 | 3.92e-27 | 105 |
| MsaG015111 | AT4G04090.1 | 33.523 | 176 | 115 | 2 | 100 | 273 | 14 | 189 | 2.38e-25 | 100 |
| MsaG015111 | AT5G48510.1 | 36.111 | 180 | 111 | 3 | 99 | 274 | 13 | 192 | 3.69e-25 | 100 |
| MsaG015111 | AT2G05330.1 | 37.762 | 143 | 87 | 2 | 129 | 269 | 34 | 176 | 5.38e-22 | 91.7 |
| MsaG015111 | AT4G08455.1 | 29.245 | 212 | 134 | 6 | 69 | 268 | 41 | 248 | 4.32e-20 | 87.8 |
| MsaG015111 | AT2G05330.2 | 37.879 | 132 | 80 | 2 | 140 | 269 | 1 | 132 | 2.54e-18 | 80.9 |
| MsaG015111 | AT3G29740.1 | 41.860 | 86 | 46 | 2 | 88 | 170 | 3 | 87 | 8.12e-13 | 63.5 |
| MsaG015111 | AT1G21780.2 | 34.058 | 138 | 86 | 3 | 104 | 241 | 155 | 287 | 1.44e-12 | 67.4 |
| MsaG015111 | AT1G21780.1 | 34.058 | 138 | 86 | 3 | 104 | 241 | 155 | 287 | 1.44e-12 | 67.4 |
| MsaG015111 | AT1G55760.1 | 33.333 | 138 | 87 | 3 | 106 | 243 | 160 | 292 | 8.06e-11 | 62.0 |
Find 44 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAGGCCTTAATATTAA+TGG | 0.173072 | 3:+82826571 | None:intergenic |
| TGCACAAAAGCTTCAAATTT+AGG | 0.202933 | 3:+82825330 | None:intergenic |
| ATTCTAGTTCTTCATGGTTT+AGG | 0.258680 | 3:+82825607 | None:intergenic |
| CAACTATGCCATTAATATTA+AGG | 0.307718 | 3:-82826579 | MsaT015111.1:CDS |
| TAGTGTCTCAAATGCATTAG+AGG | 0.315759 | 3:+82825440 | None:intergenic |
| CTCGAATTTCGCCGCGAAAT+AGG | 0.318434 | 3:+82826442 | None:intergenic |
| AGGCTGTCTCCTTCAAATCT+TGG | 0.335162 | 3:+82825394 | None:intergenic |
| GGAGAGATTCTAGTTCTTCA+TGG | 0.351873 | 3:+82825601 | None:intergenic |
| ACCTGCACATAAATCCGTTT+TGG | 0.358823 | 3:-82826154 | MsaT015111.1:intron |
| TTACAGTGGGACATTGCCTT+TGG | 0.363490 | 3:-82825568 | MsaT015111.1:CDS |
| GCCCCACATATTGTATTCCT+TGG | 0.412240 | 3:+82826553 | None:intergenic |
| AGTTCTTCATGGTTTAGGTC+AGG | 0.422615 | 3:+82825612 | None:intergenic |
| ACGGATTTATGTGCAGGTAT+AGG | 0.430061 | 3:+82826159 | None:intergenic |
| GGGCATGTTATGAAGGAGTT+AGG | 0.432141 | 3:-82826534 | MsaT015111.1:CDS |
| TGTGTGAATTTGTTCTTTGA+AGG | 0.437509 | 3:+82826215 | None:intergenic |
| GTTTCAAGAGCATTTGTGAA+TGG | 0.440008 | 3:-82825279 | MsaT015111.1:CDS |
| TTGTTCAAACCAAGATTTGA+AGG | 0.441270 | 3:-82825403 | MsaT015111.1:CDS |
| TCCTGGAGTTTCTTTACAGT+GGG | 0.444038 | 3:-82825581 | MsaT015111.1:CDS |
| CCACATATTGTATTCCTTGG+AGG | 0.460958 | 3:+82826556 | None:intergenic |
| AAAACAATCACTACTCCAAA+TGG | 0.473612 | 3:-82826469 | MsaT015111.1:CDS |
| CCTCCAAGGAATACAATATG+TGG | 0.508077 | 3:-82826556 | MsaT015111.1:CDS |
| TTCGCGGCGAAATTCGAGTA+AGG | 0.516513 | 3:-82826437 | MsaT015111.1:CDS |
| ATAGGTGGACCATGATTGCC+TGG | 0.517957 | 3:+82826177 | None:intergenic |
| ATAGTTATTGTGTTACAAGT+TGG | 0.522203 | 3:+82825636 | None:intergenic |
| CTCCTGGAGTTTCTTTACAG+TGG | 0.523154 | 3:-82825582 | MsaT015111.1:CDS |
| ACCAAAACGGATTTATGTGC+AGG | 0.533046 | 3:+82826153 | None:intergenic |
| ATATGTGGGGCATGTTATGA+AGG | 0.533797 | 3:-82826541 | MsaT015111.1:CDS |
| TCACAGTATTTCAGCAAATG+TGG | 0.541324 | 3:+82825489 | None:intergenic |
| TTGATAAGTCCAGGCAATCA+TGG | 0.543484 | 3:-82826186 | MsaT015111.1:CDS |
| CGCGAAATAGGAGAACCATT+TGG | 0.543566 | 3:+82826454 | None:intergenic |
| CAAGATTTGGCTTATCTCAG+AGG | 0.546377 | 3:-82826249 | MsaT015111.1:CDS |
| CAAATGGTTCTCCTATTTCG+CGG | 0.549333 | 3:-82826453 | MsaT015111.1:CDS |
| TGAAGAACTAGAATCTCTCC+TGG | 0.552448 | 3:-82825598 | MsaT015111.1:CDS |
| AAAACAAGAACTCACCAAAA+CGG | 0.553720 | 3:+82826140 | None:intergenic |
| ACAGATATATTGATAAGTCC+AGG | 0.558976 | 3:-82826195 | MsaT015111.1:CDS |
| CATTAATATTAAGGCCTCCA+AGG | 0.571973 | 3:-82826570 | MsaT015111.1:CDS |
| CTCCAAGGAATACAATATGT+GGG | 0.573481 | 3:-82826555 | MsaT015111.1:CDS |
| CTTATCAGCTGCTTGTGACA+AGG | 0.589102 | 3:+82825521 | None:intergenic |
| GATTTATGTGCAGGTATAGG+TGG | 0.598343 | 3:+82826162 | None:intergenic |
| TCCCACTGTAAAGAAACTCC+AGG | 0.600648 | 3:+82825580 | None:intergenic |
| ACTAATTGCACAGTTAAATG+TGG | 0.601341 | 3:+82825300 | None:intergenic |
| AGCATAATCAACATGATGAA+CGG | 0.614339 | 3:-82826511 | MsaT015111.1:CDS |
| CACTTGATGATTGTATAAGA+TGG | 0.631009 | 3:-82826308 | MsaT015111.1:CDS |
| TCCAAGGAATACAATATGTG+GGG | 0.661020 | 3:-82826554 | MsaT015111.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 82825254 | 82826620 | 82825254 | ID=MsaG015111 |
| Chr3 | mRNA | 82825254 | 82826620 | 82825254 | ID=MsaT015111.1;Parent=MsaG015111 |
| Chr3 | exon | 82825254 | 82825715 | 82825254 | ID=MsaT015111.1.exon3;Parent=MsaT015111.1 |
| Chr3 | CDS | 82825254 | 82825715 | 82825254 | ID=cds.MsaT015111.1;Parent=MsaT015111.1 |
| Chr3 | exon | 82826155 | 82826331 | 82826155 | ID=MsaT015111.1.exon2;Parent=MsaT015111.1 |
| Chr3 | CDS | 82826155 | 82826331 | 82826155 | ID=cds.MsaT015111.1;Parent=MsaT015111.1 |
| Chr3 | exon | 82826408 | 82826620 | 82826408 | ID=MsaT015111.1.exon1;Parent=MsaT015111.1 |
| Chr3 | CDS | 82826408 | 82826620 | 82826408 | ID=cds.MsaT015111.1;Parent=MsaT015111.1 |
| Gene Sequence |
| Protein sequence |
