Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015120 | XP_024637264.1 | 81.905 | 105 | 19 | 0 | 1 | 105 | 60 | 164 | 1.34e-57 | 185 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015120 | tr|G7JQ00|G7JQ00_MEDTR | 81.905 | 105 | 19 | 0 | 1 | 105 | 120 | 224 | 4.15e-57 | 185 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG015120 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015120 | MtrunA17_Chr5g0393501 | 35.354 | 99 | 61 | 1 | 10 | 105 | 143 | 241 | 1.50e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGACTACCTCATACTTT+TGG | 0.338565 | 3:+82886655 | MsaT015120.1:intron |
| GTCATGTGTTTATCAACTCT+TGG | 0.410006 | 3:-82886893 | None:intergenic |
| ACCTTTGAGGTTCCTATCGT+TGG | 0.413727 | 3:+82886517 | MsaT015120.1:CDS |
| TCCGAACATCACATTTGGTA+TGG | 0.417548 | 3:-82886770 | None:intergenic |
| CCGAACATCACATTTGGTAT+GGG | 0.451435 | 3:-82886769 | None:intergenic |
| AAGAATTTGCCAAAAGTATG+AGG | 0.469320 | 3:-82886664 | None:intergenic |
| CCAAATGTGATGTTCGGAAA+AGG | 0.475440 | 3:+82886775 | MsaT015120.1:CDS |
| CCCATACCAAATGTGATGTT+CGG | 0.477035 | 3:+82886769 | MsaT015120.1:CDS |
| TTGTGTTGCCAATGGTATTG+AGG | 0.514824 | 3:+82886810 | MsaT015120.1:CDS |
| TCTATATATTTGAGTCTCAC+AGG | 0.539594 | 3:-82886742 | None:intergenic |
| CGAACATCACATTTGGTATG+GGG | 0.552019 | 3:-82886768 | None:intergenic |
| GTCTCCGGCCTCAATACCAT+TGG | 0.575295 | 3:-82886818 | None:intergenic |
| TTTCAAGTGCAAGTGTGTCA+TGG | 0.589305 | 3:+82886849 | MsaT015120.1:CDS |
| GTTGCCAATGGTATTGAGGC+CGG | 0.597504 | 3:+82886814 | MsaT015120.1:CDS |
| ATGTGATGTTCGGAAAAGGT+TGG | 0.606400 | 3:+82886779 | MsaT015120.1:CDS |
| TCGATCGACGAAATCACCTA+AGG | 0.628108 | 3:+82886543 | MsaT015120.1:CDS |
| ACCCTATCATTCAATGAGTG+CGG | 0.636716 | 3:+82886477 | None:intergenic |
| GTCGATCGATATCCAACGAT+AGG | 0.637968 | 3:-82886529 | None:intergenic |
| CACGATTACGTAATGTTGTG+CGG | 0.649204 | 3:+82886700 | MsaT015120.1:CDS |
| TCCAACGATAGGAACCTCAA+AGG | 0.670850 | 3:-82886518 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 82886490 | 82886903 | 82886490 | ID=MsaG015120 |
| Chr3 | mRNA | 82886490 | 82886903 | 82886490 | ID=MsaT015120.1;Parent=MsaG015120 |
| Chr3 | exon | 82886490 | 82886564 | 82886490 | ID=MsaT015120.1.exon1;Parent=MsaT015120.1 |
| Chr3 | CDS | 82886490 | 82886564 | 82886490 | ID=cds.MsaT015120.1;Parent=MsaT015120.1 |
| Chr3 | exon | 82886658 | 82886903 | 82886658 | ID=MsaT015120.1.exon2;Parent=MsaT015120.1 |
| Chr3 | CDS | 82886658 | 82886903 | 82886658 | ID=cds.MsaT015120.1;Parent=MsaT015120.1 |
| Gene Sequence |
| Protein sequence |
