Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015166 | XP_003601097.2 | 90.000 | 470 | 24 | 1 | 1 | 447 | 1 | 470 | 0.0 | 865 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015166 | sp|Q7XJS0|ASHR1_ARATH | 64.399 | 441 | 153 | 1 | 12 | 448 | 35 | 475 | 0.0 | 611 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015166 | tr|G7J3L4|G7J3L4_MEDTR | 90.000 | 470 | 24 | 1 | 1 | 447 | 1 | 470 | 0.0 | 865 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000010 | MsaG015166 | 0.817742 | 4.905062e-52 | 1.016979e-49 |
| MsaG000062 | MsaG015166 | 0.822959 | 3.160737e-53 | 7.521978e-51 |
| MsaG000083 | MsaG015166 | 0.838927 | 3.960409e-57 | 1.495092e-54 |
| MsaG000113 | MsaG015166 | 0.829150 | 1.083948e-54 | 3.061477e-52 |
| MsaG000127 | MsaG015166 | 0.815047 | 1.954025e-51 | 3.781680e-49 |
| MsaG000140 | MsaG015166 | 0.828746 | 1.356520e-54 | 3.787915e-52 |
| MsaG000153 | MsaG015166 | 0.810467 | 1.944196e-50 | 3.358258e-48 |
| MsaG000262 | MsaG015166 | 0.819519 | 1.947319e-52 | 4.228615e-50 |
| MsaG000274 | MsaG015166 | 0.825739 | 7.069147e-54 | 1.814915e-51 |
| MsaG000292 | MsaG015166 | 0.825919 | 6.407891e-54 | 1.653488e-51 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015166 | MtrunA17_Chr3g0115061 | 90.000 | 470 | 24 | 1 | 1 | 447 | 1 | 470 | 0.0 | 865 |
| MsaG015166 | MtrunA17_Chr8g0375381 | 25.413 | 303 | 160 | 13 | 6 | 264 | 24 | 304 | 7.28e-14 | 73.2 |
| MsaG015166 | MtrunA17_Chr3g0128421 | 28.205 | 156 | 95 | 4 | 159 | 312 | 459 | 599 | 8.74e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015166 | AT2G17900.1 | 64.399 | 441 | 153 | 1 | 12 | 448 | 40 | 480 | 0.0 | 612 |
Find 81 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCAACACTGAATTGGGT+AGG | 0.637837 | 3:+83367602 | None:intergenic |
| AAAGGATACAAAACCTGACA+TGG | 0.639706 | 3:+83368764 | None:intergenic |
| AAGCACTGTGACTCGACAAA+AGG | 0.521126 | 3:-83366855 | MsaT015166.1:CDS |
| AAGGAAATTGCAAGATGGCA+AGG | 0.594988 | 3:-83368432 | MsaT015166.1:intron |
| AAGTACCACTTTAGAACAGA+GGG | 0.532968 | 3:+83366796 | None:intergenic |
| AAGTTTGAGCATCAACCGTA+TGG | 0.550663 | 3:+83368463 | None:intergenic |
| AATATTGCAAGCCAAGCAAA+GGG | 0.578542 | 3:+83364476 | None:intergenic |
| AATCTGTTACACCATCCATA+CGG | 0.635943 | 3:-83368478 | MsaT015166.1:CDS |
| ACATGTGCCTAAAGGGACTG+AGG | 0.674349 | 3:-83367545 | MsaT015166.1:intron |
| AGACATGTCTGACATCAAAG+AGG | 0.653286 | 3:-83368012 | MsaT015166.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 83363836 | 83369104 | 83363836 | ID=MsaG015166 |
| Chr3 | mRNA | 83363836 | 83369104 | 83363836 | ID=MsaT015166.1;Parent=MsaG015166 |
| Chr3 | exon | 83363836 | 83363998 | 83363836 | ID=MsaT015166.1.exon14;Parent=MsaT015166.1 |
| Chr3 | CDS | 83363836 | 83363998 | 83363836 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83364454 | 83364517 | 83364454 | ID=MsaT015166.1.exon13;Parent=MsaT015166.1 |
| Chr3 | CDS | 83364454 | 83364517 | 83364454 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83365334 | 83365403 | 83365334 | ID=MsaT015166.1.exon12;Parent=MsaT015166.1 |
| Chr3 | CDS | 83365334 | 83365403 | 83365334 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83366041 | 83366147 | 83366041 | ID=MsaT015166.1.exon11;Parent=MsaT015166.1 |
| Chr3 | CDS | 83366041 | 83366147 | 83366041 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83366324 | 83366443 | 83366324 | ID=MsaT015166.1.exon10;Parent=MsaT015166.1 |
| Chr3 | CDS | 83366324 | 83366443 | 83366324 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83366599 | 83366689 | 83366599 | ID=MsaT015166.1.exon9;Parent=MsaT015166.1 |
| Chr3 | CDS | 83366599 | 83366689 | 83366599 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83366802 | 83366906 | 83366802 | ID=MsaT015166.1.exon8;Parent=MsaT015166.1 |
| Chr3 | CDS | 83366802 | 83366906 | 83366802 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83367546 | 83367627 | 83367546 | ID=MsaT015166.1.exon7;Parent=MsaT015166.1 |
| Chr3 | CDS | 83367546 | 83367627 | 83367546 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83367717 | 83367805 | 83367717 | ID=MsaT015166.1.exon6;Parent=MsaT015166.1 |
| Chr3 | CDS | 83367717 | 83367805 | 83367717 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83367914 | 83368032 | 83367914 | ID=MsaT015166.1.exon5;Parent=MsaT015166.1 |
| Chr3 | CDS | 83367914 | 83368032 | 83367914 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83368119 | 83368173 | 83368119 | ID=MsaT015166.1.exon4;Parent=MsaT015166.1 |
| Chr3 | CDS | 83368119 | 83368173 | 83368119 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83368433 | 83368567 | 83368433 | ID=MsaT015166.1.exon3;Parent=MsaT015166.1 |
| Chr3 | CDS | 83368433 | 83368567 | 83368433 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83368778 | 83368911 | 83368778 | ID=MsaT015166.1.exon2;Parent=MsaT015166.1 |
| Chr3 | CDS | 83368778 | 83368911 | 83368778 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Chr3 | exon | 83369074 | 83369104 | 83369074 | ID=MsaT015166.1.exon1;Parent=MsaT015166.1 |
| Chr3 | CDS | 83369074 | 83369104 | 83369074 | ID=cds.MsaT015166.1;Parent=MsaT015166.1 |
| Gene Sequence |
>MsaT015166.1 ATGGAGGAATTGCAACAAGCACTGAAGAATTGGGAAGTGATTATTAGCCAAGAGCCCTATGTTTGTGTTCCAACTCAAAAAAGGTGTGATGGATGTTTCTCAACAACTAACCTTAGCAAATGCTCACGTTGTCAAGTTGTTTGGTATTGCGGAACACCATGTCAGAAATCGGAGTGGAAGTTGCATCGTCTTGAATGTCAAGCACTCTCAAGGCTAGACAACCATAAGAGAAAATCTGTTACACCATCCATACGGTTGATGCTCAAACTTTATCTTCGAAGGAAATTGCAAGATGGCAAGATCATTCCAAGCACTGCTATGGACAACTACAAGTTAGTGGACGCATTAGTGGCTCACATGTCTGACATCAAAGAGGAGCAGCTGGTGTTGTATGCACAGATGGCTAATCTTGTACACTTAATACTGCAATGGCCTGAAATCAATATTAAAGAGATTGCAGAAATTTTTTCCAAGTTTGCATGTAATGCGCATACCATTTGTGACAGTGAGTTAAGACCTCTGGGAACAGGATTGTATCCTGTTGTTTCCATTATCAATCACAGCTGCCTACCCAATTCAGTGTTGGTTTTTGAAGGAAGGGTAGCATCGGTACGTGCTGTGCAACATGTGCCTAAAGGGACTGAGGTATTGATAAGTTATATAGAGACAGCTGGAAGCACTGTGACTCGACAAAAGGCTCTCAGAGAGCAGTACCTATTCACTTGTGTTTGTCCCCTCTGTTCTAAAGTGGGTCAGTATGAAGATGTTCGAGAAAATGCAATTCTAGAAGGATACAGATGCAAAAATGAAACATGTGATGGTTTCTTGCTCCGCACAACTGATGGAAAAGCGTTTCAATGCCAAGAATGTGGGTTAGTAAGAGACAAGGAAGAGATAAAGCAAATTGCAACTGAAATAAAATTTCTATCAGAAGAAGCTTCTAAGCCTTCTTCTAGTGGCAATTATCATGAAGCTATTTCCATATATAAAATGATCGAGAAACTGCAAACAAAACTATATCACCCTTTCTCGATCAATTTGATGCAAACACGAGAGGCGATTTTGAAGTCATTAATGAAGCTGGAGTGTTGGAGAGAAGCTTTAGCATATTGCAAATTGACCATACCAATCTATCAAAGAGTGTATCCAGCTGTTCACCCTTTGCTTGGCTTGCAATATTATACTTGTGGAAAACTTGAATGGTATCTTGGAGACACAGAGGAAGCTATTAAATCATTGACCAAGGCAGTGGATATACTAAGGATTACTCATGGTACAAAGACTCCATTAGTGAAGGAACTATTGATGATGTTGGAGGAAGCCCGTGCAGAAGCCTCTTACAAATTGTCATCAAAAGAAGAGTAG |
| Protein sequence |
>MsaT015166.1 MEELQQALKNWEVIISQEPYVCVPTQKRCDGCFSTTNLSKCSRCQVVWYCGTPCQKSEWKLHRLECQALSRLDNHKRKSVTPSIRLMLKLYLRRKLQDGKIIPSTAMDNYKLVDALVAHMSDIKEEQLVLYAQMANLVHLILQWPEINIKEIAEIFSKFACNAHTICDSELRPLGTGLYPVVSIINHSCLPNSVLVFEGRVASVRAVQHVPKGTEVLISYIETAGSTVTRQKALREQYLFTCVCPLCSKVGQYEDVRENAILEGYRCKNETCDGFLLRTTDGKAFQCQECGLVRDKEEIKQIATEIKFLSEEASKPSSSGNYHEAISIYKMIEKLQTKLYHPFSINLMQTREAILKSLMKLECWREALAYCKLTIPIYQRVYPAVHPLLGLQYYTCGKLEWYLGDTEEAIKSLTKAVDILRITHGTKTPLVKELLMMLEEARAEASYKLSSKEE* |
