AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015196

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015196 MtrunA17_Chr3g0115271 97.311 595 14 1 1 595 1 593 0.0 1197
MsaG015196 MtrunA17_Chr5g0424931 44.846 553 250 14 19 560 19 527 2.99e-146 433
MsaG015196 MtrunA17_Chr2g0326761 43.494 561 281 14 15 556 7 550 2.01e-129 395
MsaG015196 MtrunA17_Chr5g0417281 40.598 569 261 14 16 564 8 519 7.63e-128 385
MsaG015196 MtrunA17_Chr7g0264171 42.857 525 249 16 15 503 27 536 1.26e-124 380
MsaG015196 MtrunA17_Chr4g0028971 47.991 448 215 10 16 450 9 451 1.99e-124 382
MsaG015196 MtrunA17_Chr1g0181211 41.426 589 288 18 16 575 24 584 6.36e-122 375
MsaG015196 MtrunA17_Chr1g0199681 43.340 473 200 13 16 450 91 533 1.71e-118 367
MsaG015196 MtrunA17_Chr1g0178011 37.085 542 221 12 7 541 3 431 2.51e-107 330
MsaG015196 MtrunA17_Chr5g0436761 39.696 461 220 9 10 462 3 413 6.84e-100 311
MsaG015196 MtrunA17_Chr2g0277461 35.856 555 240 15 11 558 5 450 8.17e-99 308
MsaG015196 MtrunA17_Chr2g0289221 44.475 362 143 8 9 362 10 321 1.54e-91 286
MsaG015196 MtrunA17_Chr5g0417211 44.011 359 150 8 9 362 2 314 6.69e-90 283
MsaG015196 MtrunA17_Chr7g0264161 43.658 339 147 7 29 362 2 301 1.49e-85 268
MsaG015196 MtrunA17_Chr5g0424741 33.099 568 249 19 16 510 9 518 5.59e-75 248
MsaG015196 MtrunA17_Chr2g0277531 39.665 358 149 14 16 362 17 318 3.39e-72 234
MsaG015196 MtrunA17_Chr5g0424801 35.897 390 182 11 16 393 9 342 6.16e-70 230
MsaG015196 MtrunA17_Chr8g0376771 37.778 405 199 8 7 399 11 374 6.52e-69 240
MsaG015196 MtrunA17_Chr5g0425261 36.579 380 177 10 16 383 9 336 7.08e-69 228
MsaG015196 MtrunA17_Chr2g0276781 37.755 392 201 8 16 401 20 374 1.11e-68 235
MsaG015196 MtrunA17_Chr2g0282961 34.804 408 216 5 5 401 39 407 2.69e-68 233
MsaG015196 MtrunA17_Chr2g0285461 37.656 401 206 7 7 399 11 375 1.18e-67 236
MsaG015196 MtrunA17_Chr4g0009751 36.387 393 201 8 17 401 17 368 5.42e-66 226
MsaG015196 MtrunA17_Chr1g0156321 38.832 394 185 11 15 397 39 387 2.99e-65 229
MsaG015196 MtrunA17_Chr3g0109391 36.990 392 203 7 16 399 23 378 4.25e-65 228
MsaG015196 MtrunA17_Chr5g0433301 36.735 392 204 7 16 399 21 376 2.05e-64 226
MsaG015196 MtrunA17_Chr8g0385791 34.491 432 219 9 19 427 59 449 3.17e-64 226
MsaG015196 MtrunA17_Chr4g0046371 35.750 400 199 9 19 401 38 396 9.64e-63 219
MsaG015196 MtrunA17_Chr7g0239061 34.910 444 237 15 16 447 11 414 3.90e-62 217
MsaG015196 MtrunA17_Chr4g0070181 35.696 381 196 6 15 386 19 359 1.02e-60 218
MsaG015196 MtrunA17_Chr2g0326281 35.468 406 193 10 19 397 56 419 2.02e-59 212
MsaG015196 MtrunA17_Chr4g0029671 33.920 398 213 7 19 401 54 416 7.07e-58 207
MsaG015196 MtrunA17_Chr8g0346711 35.459 392 207 9 16 401 26 377 9.76e-58 205
MsaG015196 MtrunA17_Chr5g0425661 48.515 202 96 4 11 207 29 227 2.55e-54 184
MsaG015196 MtrunA17_Chr2g0301251 45.161 217 106 3 16 223 20 232 5.06e-54 198
MsaG015196 MtrunA17_Chr8g0389081 45.370 216 112 4 11 220 24 239 1.02e-50 189
MsaG015196 MtrunA17_Chr8g0356211 28.626 262 148 6 125 386 2 224 4.52e-30 120
MsaG015196 MtrunA17_Chr5g0425651 40.625 128 59 4 277 395 2 121 2.68e-24 98.2
MsaG015196 MtrunA17_Chr5g0424791 39.865 148 77 4 68 213 14 151 8.52e-22 92.0
MsaG015196 MtrunA17_Chr5g0419861 41.379 116 57 5 56 164 11 122 1.88e-14 70.5
MsaG015196 MtrunA17_Chr1g0178071 36.752 117 68 4 105 217 65 179 3.78e-12 67.8
MsaG015196 MtrunA17_Chr4g0072581 70.213 47 13 1 187 232 5 51 1.04e-11 60.8
MsaG015196 MtrunA17_Chr8g0345771 36.364 110 65 2 112 217 167 275 1.96e-11 66.6
MsaG015196 MtrunA17_Chr5g0422501 33.051 118 71 3 97 207 217 333 8.12e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015196 AT1G77850.2 47.950 561 269 11 12 562 12 559 1.55e-164 482
MsaG015196 AT1G77850.1 47.950 561 269 11 12 562 12 559 2.03e-164 482
MsaG015196 AT4G30080.1 42.376 564 284 14 16 561 16 556 1.21e-121 375
MsaG015196 AT2G28350.2 46.389 457 209 10 15 447 6 450 7.04e-120 370
MsaG015196 AT2G28350.1 46.389 457 209 10 15 447 6 450 8.44e-120 370
MsaG015196 AT5G37020.2 37.101 407 211 8 16 413 19 389 7.81e-70 240
MsaG015196 AT5G37020.1 37.101 407 211 8 16 413 19 389 1.84e-69 240
MsaG015196 AT1G30330.3 38.366 404 201 8 8 399 10 377 7.54e-69 240
MsaG015196 AT1G30330.2 38.366 404 201 8 8 399 10 377 7.54e-69 240
MsaG015196 AT1G30330.1 38.250 400 203 7 8 399 12 375 1.03e-68 240
MsaG015196 AT2G33860.1 33.983 462 244 8 19 460 54 474 1.34e-68 234
MsaG015196 AT1G59750.2 37.692 390 198 10 19 401 22 373 5.55e-66 228
MsaG015196 AT1G59750.4 37.692 390 198 10 19 401 22 373 5.87e-66 228
MsaG015196 AT1G59750.1 37.692 390 198 10 19 401 22 373 6.42e-66 228
MsaG015196 AT1G59750.3 37.692 390 198 10 19 401 22 373 6.42e-66 228
MsaG015196 AT2G46530.1 35.309 405 211 8 12 406 14 377 2.29e-63 219
MsaG015196 AT4G23980.2 36.702 376 187 10 16 382 9 342 3.31e-63 220
MsaG015196 AT4G23980.1 36.702 376 187 10 16 382 9 342 4.52e-63 219
MsaG015196 AT2G46530.3 35.309 405 211 8 12 406 35 398 5.03e-63 219
MsaG015196 AT5G60450.2 34.670 424 222 9 12 419 34 418 1.99e-62 220
MsaG015196 AT5G60450.1 35.012 417 216 9 19 419 65 442 2.47e-61 217
MsaG015196 AT1G19850.2 37.468 395 188 12 16 397 40 388 2.67e-61 219
MsaG015196 AT1G19850.1 37.468 395 188 12 16 397 51 399 3.06e-61 218
MsaG015196 AT3G61830.1 34.726 383 206 6 16 393 22 365 1.01e-60 212
MsaG015196 AT5G62000.2 33.487 433 222 10 19 427 61 451 3.56e-60 215
MsaG015196 AT5G62000.5 33.487 433 222 10 19 427 61 451 3.56e-60 215
MsaG015196 AT5G62000.3 33.487 433 222 10 19 427 61 451 3.56e-60 215
MsaG015196 AT5G62000.1 33.487 433 222 10 19 427 61 451 3.56e-60 215
MsaG015196 AT5G62000.4 33.487 433 222 10 19 427 61 451 5.61e-60 214
MsaG015196 AT1G35520.1 31.481 486 257 15 11 465 19 459 9.54e-57 201
MsaG015196 AT1G34410.1 32.083 480 246 18 19 465 27 459 7.53e-56 199
MsaG015196 AT1G34310.1 34.761 397 204 11 19 406 27 377 2.89e-55 197
MsaG015196 AT1G35240.1 34.596 396 202 12 19 404 27 375 8.28e-55 196
MsaG015196 AT1G19220.1 45.161 217 106 3 16 223 20 232 4.88e-54 199
MsaG015196 AT5G20730.1 45.161 217 106 3 16 223 21 233 3.15e-53 196
MsaG015196 AT5G20730.2 45.161 217 106 3 16 223 21 233 3.27e-53 196
MsaG015196 AT5G20730.3 45.161 217 106 3 16 223 21 233 3.57e-53 196
MsaG015196 AT1G34390.1 33.753 397 206 12 19 406 27 375 2.29e-52 189
MsaG015196 AT2G33860.2 35.028 354 182 6 117 460 2 317 5.13e-52 185
MsaG015196 AT1G35540.1 33.165 395 213 12 19 406 27 377 2.55e-51 186
MsaG015196 AT5G37020.3 38.000 300 147 6 117 413 31 294 5.11e-51 187
MsaG015196 AT3G61830.2 34.545 330 177 5 64 393 24 314 1.30e-48 178
MsaG015196 AT1G34170.1 30.548 383 206 11 14 384 20 354 4.35e-45 167
MsaG015196 AT2G46530.2 35.786 299 144 8 114 406 34 290 1.01e-42 161
MsaG015196 ATMG00940.1 50.000 154 71 3 204 357 1 148 3.82e-39 141
MsaG015196 AT1G34170.2 28.982 383 186 11 14 384 20 328 7.59e-39 149
MsaG015196 AT1G34170.3 28.982 383 186 11 14 384 20 328 2.78e-38 149
MsaG015196 AT1G43950.1 38.725 204 111 7 19 216 27 222 1.16e-34 131
MsaG015196 AT3G61970.1 34.454 119 73 3 104 217 9 127 1.86e-12 68.9
MsaG015196 AT2G36080.3 37.273 110 65 2 112 217 33 142 2.73e-12 67.4
MsaG015196 AT2G36080.1 37.273 110 65 2 112 217 33 142 3.20e-12 67.4
MsaG015196 AT2G36080.4 37.273 110 65 2 112 217 33 142 3.48e-12 65.5
MsaG015196 AT2G36080.2 37.273 110 65 2 112 217 33 142 3.48e-12 65.5
MsaG015196 AT2G46870.1 33.071 127 79 2 111 232 29 154 1.96e-11 65.9
MsaG015196 AT1G01030.2 33.613 119 73 2 103 216 42 159 2.31e-11 65.9
MsaG015196 AT1G01030.1 33.613 119 73 2 103 216 42 159 2.45e-11 66.2
MsaG015196 AT4G01500.1 38.542 96 50 2 113 205 32 121 2.47e-11 65.9
MsaG015196 AT3G25730.1 37.500 104 57 4 117 214 183 284 8.58e-11 64.3

Find 186 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCAACTGTAGAGGCTATT+TGG 0.262417 3:-83767019 None:intergenic
CTGTTCCTGATCGTGGGGCT+TGG 0.289614 3:+83765343 MsaT015196.1:CDS
GCGATAGCTCGCCAGAGTTT+GGG 0.291734 3:-83764361 None:intergenic
AAAAGGACTAGAGGGGTATC+TGG 0.298766 3:+83766459 MsaT015196.1:CDS
GGCGATAGCTCGCCAGAGTT+TGG 0.304479 3:-83764362 None:intergenic
AAATCCCCTCCGTCAATTCT+AGG 0.308448 3:+83764398 MsaT015196.1:CDS
TCCTCCGTTGGATTATAGTA+TGG 0.314074 3:+83764738 MsaT015196.1:CDS
TCCGGTGGCGGTTGCGGAGG+CGG 0.344489 3:+83765122 MsaT015196.1:CDS
CAGAGTAGTTTGCCTTCCTT+TGG 0.347823 3:+83766768 MsaT015196.1:CDS
TGATAACTTCCGTCTTTGTA+TGG 0.363524 3:+83766647 MsaT015196.1:CDS
TTGGAAGTTTGCAACACTTC+AGG 0.367022 3:-83766366 None:intergenic
TCCAGGACTACTTGGTTTCA+AGG 0.368052 3:+83765303 MsaT015196.1:CDS
CCAAATAGCTGAAAAGTTAC+AGG 0.369551 3:-83766837 None:intergenic
GGCGGCGGAAATGGCAGCAC+AGG 0.371956 3:+83765140 MsaT015196.1:CDS
GGTTGCGGAGGCGGCGGAAA+TGG 0.372996 3:+83765131 MsaT015196.1:CDS
TGGAGACTGATGATTCTTCC+AGG 0.375310 3:+83765286 MsaT015196.1:CDS
CTGCTGGCATGCAGGGAGCC+AGG 0.375982 3:+83766583 MsaT015196.1:CDS
TTGGAGACGGTGGTAGTACT+TGG 0.385584 3:-83764318 None:intergenic
TTTGGCAGCAACACAGTGCC+GGG 0.386977 3:+83766678 MsaT015196.1:CDS
CTTTGTATGGGTAACTCCTT+TGG 0.387691 3:+83766660 MsaT015196.1:CDS
TTGTTGATTACTGATGTTCA+TGG 0.389456 3:+83764778 MsaT015196.1:CDS
GTTTCAAGGTGTAGTGTCTC+AGG 0.398107 3:+83765317 MsaT015196.1:CDS
CTGGCTCCCTGCATGCCAGC+AGG 0.411242 3:-83766582 None:intergenic
AGGTTTCTGTTCCTGATCGT+GGG 0.413423 3:+83765337 MsaT015196.1:CDS
GTGAACCCTGTGATAGAAAA+GGG 0.417152 3:-83766507 None:intergenic
CGCCGGCATTTGTTGACCAC+CGG 0.418386 3:+83764841 MsaT015196.1:CDS
AAATTGCTCGACAGGCTTGA+TGG 0.418640 3:+83766990 MsaT015196.1:CDS
ATGATTCTTCCAGGACTACT+TGG 0.424084 3:+83765295 MsaT015196.1:CDS
AGAAGAGGTGAGAGATTGTT+CGG 0.425048 3:-83764466 None:intergenic
GTTGTCTCCGGTGGCGGTTG+CGG 0.431234 3:+83765116 MsaT015196.1:CDS
CTGCCAAGGGCTGACCCACT+TGG 0.431622 3:-83766385 None:intergenic
CAAGTGGGTCAGCCCTTGGC+AGG 0.434174 3:+83766386 MsaT015196.1:CDS
CAGGTTTCTGTTCCTGATCG+TGG 0.441155 3:+83765336 MsaT015196.1:CDS
GGCAAACTACTCTGGCTCTC+TGG 0.444672 3:-83766759 None:intergenic
AGCTGAAAAGTTACAGGCCC+GGG 0.451430 3:-83766831 None:intergenic
AAATGGCAGCACAGGGGATG+GGG 0.452139 3:+83765148 MsaT015196.1:CDS
AACGCTAAGAAGCTTGTTGC+TGG 0.453003 3:+83764880 MsaT015196.1:CDS
AGTGAACCCTGTGATAGAAA+AGG 0.454541 3:-83766508 None:intergenic
GCCGCCTCCGCAACCGCCAC+CGG 0.456465 3:-83765123 None:intergenic
GGGGAAGTTGTCTCCGGTGG+CGG 0.456900 3:+83765110 MsaT015196.1:CDS
GGTGGCGGTTGCGGAGGCGG+CGG 0.459780 3:+83765125 MsaT015196.1:CDS
GAAATGGCAGCACAGGGGAT+GGG 0.460169 3:+83765147 MsaT015196.1:CDS
GGAAATGGCAGCACAGGGGA+TGG 0.461216 3:+83765146 MsaT015196.1:CDS
CAAAGGAAGGCAAACTACTC+TGG 0.464413 3:-83766767 None:intergenic
TCCTGGAATGAGAATCAAAA+TGG 0.464829 3:+83765260 MsaT015196.1:CDS
GGGGATTTGCACGGCAGCGC+CGG 0.466220 3:-83764383 None:intergenic
GGTGGATGAAGAAGAGAAGC+TGG 0.468766 3:+83765056 MsaT015196.1:CDS
CTTACTCAAAATTGCTCGAC+AGG 0.469093 3:+83766982 MsaT015196.1:CDS
AAAGGACTAGAGGGGTATCT+GGG 0.471476 3:+83766460 MsaT015196.1:CDS
CGATCTGTGGAGTGAGAAGT+AGG 0.471915 3:+83765034 MsaT015196.1:CDS
CCTCTCCAAGCCCCACGATC+AGG 0.472164 3:-83765348 None:intergenic
CACAGGAGATGACAGCAGTA+AGG 0.472700 3:+83766914 MsaT015196.1:CDS
ACCATTTGAACCACTTTCAA+CGG 0.473718 3:-83766883 None:intergenic
ATCGGAGCAAACACCGCCAC+CGG 0.479208 3:-83764992 None:intergenic
CCATTTGAACCACTTTCAAC+GGG 0.480213 3:-83766882 None:intergenic
CTGATGTTCATGGTTTGACT+TGG 0.481505 3:+83764788 MsaT015196.1:CDS
CTTTGGCAGCAACACAGTGC+CGG 0.482301 3:+83766677 MsaT015196.1:CDS
CCACCGGCGCTACTCCGATT+CGG 0.482322 3:-83764976 None:intergenic
CCCGTTGAAAGTGGTTCAAA+TGG 0.482554 3:+83766882 MsaT015196.1:CDS
ATTGTTTCTGATAGATTACA+TGG 0.486179 3:+83766337 MsaT015196.1:intron
AATCCCCTCCGTCAATTCTA+GGG 0.486899 3:+83764399 MsaT015196.1:CDS
CGGGAAGGGGAAGTTGTCTC+CGG 0.490275 3:+83765104 MsaT015196.1:CDS
ACCTTGAAACCAAGTAGTCC+TGG 0.491621 3:-83765304 None:intergenic
GAGGAAATGAACGGCGGAGA+CGG 0.491674 3:-83764515 None:intergenic
GAAGTTATCACCTGGAAAGT+TGG 0.498091 3:-83766634 None:intergenic
AGAGAAGCTGGTGGATGAGA+AGG 0.506899 3:+83765068 MsaT015196.1:CDS
CTTCCAAGTGGGTCAGCCCT+TGG 0.507741 3:+83766382 MsaT015196.1:CDS
GGGTTCACTAATTCAACAAT+GGG 0.507819 3:+83766522 MsaT015196.1:CDS
CTTGGTCCATGTGACCTTGA+GGG 0.511485 3:-83764432 None:intergenic
AATCGGAGTAGCGCCGGTGG+CGG 0.511889 3:+83764979 MsaT015196.1:CDS
ACAGGAGATGACAGCAGTAA+GGG 0.514015 3:+83766915 MsaT015196.1:CDS
CACCGTTGTTCGCATCAGAC+GGG 0.518986 3:-83764675 None:intergenic
ATGAGTGTTACTTGGTGTCC+TGG 0.519012 3:+83765243 MsaT015196.1:CDS
GAGACGGTGGTAGTACTTGG+CGG 0.520685 3:-83764315 None:intergenic
CTGTTCGATTAGTTCCGAAT+CGG 0.520734 3:+83764962 MsaT015196.1:CDS
TAGGGACGAGAGAGAAGAAG+AGG 0.523309 3:-83764481 None:intergenic
GAAAGAAGTTCCACCTGCCA+AGG 0.525099 3:-83766399 None:intergenic
GTGGGTCAGCCCTTGGCAGG+TGG 0.528173 3:+83766389 MsaT015196.1:CDS
GCGGCGGAAATGGCAGCACA+GGG 0.528509 3:+83765141 MsaT015196.1:CDS
GTGGTGTATTATCCGAGAGC+GGG 0.529625 3:+83765177 MsaT015196.1:CDS
GTCTCCGGTGGCGGTTGCGG+AGG 0.531959 3:+83765119 MsaT015196.1:CDS
CTACTTCCCTCAAGGTCACA+TGG 0.532844 3:+83764426 MsaT015196.1:CDS
AACCGGTGGTCAACAAATGC+CGG 0.533689 3:-83764843 None:intergenic
CCACCGTTGTTCGCATCAGA+CGG 0.534591 3:-83764676 None:intergenic
AACTGCAACTCAATGAAACC+CGG 0.535247 3:+83766813 MsaT015196.1:CDS
ACAGAACAGCCCGTTGAAAG+TGG 0.538177 3:+83766873 MsaT015196.1:CDS
AACGGAAAATAGATCATGCC+TGG 0.538278 3:-83766601 None:intergenic
GAGACGGAGCAGAGAATGTA+GGG 0.539234 3:-83764499 None:intergenic
GATAACTTCCGTCTTTGTAT+GGG 0.539535 3:+83766648 MsaT015196.1:CDS
GTAGTAAACCCTAGAATTGA+CGG 0.541390 3:-83764407 None:intergenic
GAAAGCGGAGGTTGTGGATG+CGG 0.543290 3:+83765218 MsaT015196.1:CDS
CCCCATTGAAAAGGACTAGA+GGG 0.543634 3:+83766451 MsaT015196.1:CDS
TCTGATGCGAACAACGGTGG+TGG 0.543727 3:+83764679 MsaT015196.1:CDS
TGTTGACCACCGGTTGGAGT+AGG 0.544509 3:+83764851 MsaT015196.1:CDS
TGATGTTCATGGTTTGACTT+GGG 0.545373 3:+83764789 MsaT015196.1:CDS
AAACTCTGGCGAGCTATCGC+CGG 0.546085 3:+83764364 MsaT015196.1:CDS
AAATCCGACCACCCCGCTCT+CGG 0.548356 3:-83765189 None:intergenic
TAGCTGAAAAGTTACAGGCC+CGG 0.548450 3:-83766832 None:intergenic
TTATCCGAGAGCGGGGTGGT+CGG 0.550098 3:+83765185 MsaT015196.1:CDS
ATGCGGCGATGAGTGTTACT+TGG 0.550397 3:+83765235 MsaT015196.1:CDS
ACAAAATCCGTGCCAAAGGA+AGG 0.551308 3:-83766780 None:intergenic
CCACACTCAACCCCAAACTC+TGG 0.551845 3:+83764350 MsaT015196.1:CDS
TGTTCGGAAGTGAAGTAGCT+TGG 0.552094 3:-83764450 None:intergenic
CTGTAGAGGCTATTTGGCAC+TGG 0.552669 3:-83767013 None:intergenic
GGAGACGGAGCAGAGAATGT+AGG 0.553295 3:-83764500 None:intergenic
GTTCACAAACCTACTCCAAC+CGG 0.553748 3:-83764860 None:intergenic
AGGGTTTACTACTTCCCTCA+AGG 0.555346 3:+83764418 MsaT015196.1:CDS
CGGCACTGTGTTGCTGCCAA+AGG 0.556216 3:-83766676 None:intergenic
GGCATTTGTTGACCACCGGT+TGG 0.557182 3:+83764845 MsaT015196.1:CDS
CCGTCGTCAGCTTCAATCAC+CGG 0.562074 3:-83764619 None:intergenic
TAGTTTGCCTTCCTTTGGCA+CGG 0.562608 3:+83766773 MsaT015196.1:CDS
TGTGTTGAAAGCGGAGGTTG+TGG 0.562873 3:+83765212 MsaT015196.1:CDS
ATCAACAAATTCTGCAACGG+AGG 0.565435 3:-83764763 None:intergenic
CAAAGACGGAAGTTATCACC+TGG 0.565893 3:-83766642 None:intergenic
CCTGATCGTGGGGCTTGGAG+AGG 0.567198 3:+83765348 MsaT015196.1:CDS
AATGCCGGCGCGGCGTTCCA+CGG 0.569609 3:-83764828 None:intergenic
AGGCATTAACAACCCTCTTG+AGG 0.570796 3:+83766953 MsaT015196.1:CDS
TTTACCGTGGAACGCCGCGC+CGG 0.571717 3:+83764824 MsaT015196.1:CDS
AGATTGTGTCTTCCGATCTG+TGG 0.572758 3:+83765021 MsaT015196.1:CDS
ACTAGAGGTGCAATGTTCAT+CGG 0.573435 3:+83764928 MsaT015196.1:CDS
GGGCTTGGAGAGGTTCACCT+TGG 0.573691 3:+83765358 MsaT015196.1:CDS
GGAAAGGGCGTATGAAGGGA+AGG 0.577013 3:-83766429 None:intergenic
ACAGGGGATGGGGTTTGAAG+TGG 0.577463 3:+83765158 MsaT015196.1:CDS
GTTCCGAATCGGAGTAGCGC+CGG 0.582911 3:+83764973 MsaT015196.1:CDS
CCAGAGTTTGGGGTTGAGTG+TGG 0.583941 3:-83764350 None:intergenic
TCAAATGGTACTGTTAGCAC+AGG 0.585129 3:+83766897 MsaT015196.1:CDS
CCGAACAAAATCCGTGCCAA+AGG 0.585733 3:-83766784 None:intergenic
AACGTTGGCAGTTCTCACTC+TGG 0.586514 3:+83766726 MsaT015196.1:CDS
GCTTGGTCCATGTGACCTTG+AGG 0.587306 3:-83764433 None:intergenic
TTGAAGCTGACGACGGTGAG+AGG 0.587325 3:+83764626 MsaT015196.1:CDS
CGATAGCTCGCCAGAGTTTG+GGG 0.589326 3:-83764360 None:intergenic
GTATCTGGGGTGTTAACTGA+TGG 0.592887 3:+83766474 MsaT015196.1:CDS
GCTTCAATCACCGGAATCCG+TGG 0.594157 3:-83764610 None:intergenic
AAGCTTAGCGAAAACCTCAT+CGG 0.601270 3:-83764551 None:intergenic
CCGAATCGGAGTAGCGCCGG+TGG 0.601878 3:+83764976 MsaT015196.1:CDS
AATGAGAATCAAAATGGCTG+TGG 0.607672 3:+83765266 MsaT015196.1:CDS
GAGTAAGTAGAATCCTCAAG+AGG 0.608623 3:-83766966 None:intergenic
GGTTTCTGTTCCTGATCGTG+GGG 0.610340 3:+83765338 MsaT015196.1:CDS
AGGGTTCACTAATTCAACAA+TGG 0.612370 3:+83766521 MsaT015196.1:CDS
ATCGAATCAGCACAAAACCT+CGG 0.612548 3:-83764712 None:intergenic
GTAATCAACAAATTCTGCAA+CGG 0.612614 3:-83764766 None:intergenic
CCAAATAGCCTCTACAGTTG+AGG 0.612889 3:+83767019 MsaT015196.1:CDS
AAGGACTAGAGGGGTATCTG+GGG 0.618552 3:+83766461 MsaT015196.1:CDS
CTACTTCTCACTCCACAGAT+CGG 0.620895 3:-83765033 None:intergenic
ACTGCAACTCAATGAAACCC+GGG 0.624047 3:+83766814 MsaT015196.1:CDS
CGGATCGGCGAGGAAATGAA+CGG 0.627023 3:-83764524 None:intergenic
TTGGCAGCAACACAGTGCCG+GGG 0.627502 3:+83766679 MsaT015196.1:CDS
TTAACAGTGAAATCATTCAA+CGG 0.636083 3:-83764583 None:intergenic
ATTGACGGAGGGGATTTGCA+CGG 0.638032 3:-83764392 None:intergenic
AAGGGCTGCAACGAAACTGA+AGG 0.639246 3:+83766933 MsaT015196.1:CDS
GGATGAAGAAGAGAAGCTGG+TGG 0.642912 3:+83765059 MsaT015196.1:CDS
AGTAAGTAGAATCCTCAAGA+GGG 0.643282 3:-83766965 None:intergenic
ATAGCACGCCTCAACTGTAG+AGG 0.648425 3:-83767027 None:intergenic
AGGAGTTACCCATACAAAGA+CGG 0.649086 3:-83766656 None:intergenic
AAAGTACTGTAACTCAACAA+TGG 0.650302 3:-83766558 None:intergenic
GATCTGTGGAGTGAGAAGTA+GGG 0.650471 3:+83765035 MsaT015196.1:CDS
TTGTTTCTGATAGATTACAT+GGG 0.652931 3:+83766338 MsaT015196.1:intron
AAAGAAGTTCCACCTGCCAA+GGG 0.653496 3:-83766398 None:intergenic
GAGTTTCGTCACATTTACCG+TGG 0.654474 3:+83764811 MsaT015196.1:CDS
GAAGGGGAAGTTGTCTCCGG+TGG 0.654859 3:+83765107 MsaT015196.1:CDS
ACCCCGTCTGATGCGAACAA+CGG 0.656934 3:+83764673 MsaT015196.1:CDS
TAAACCCTAGAATTGACGGA+GGG 0.662879 3:-83764403 None:intergenic
TGGTGTATTATCCGAGAGCG+GGG 0.665380 3:+83765178 MsaT015196.1:CDS
GGGGTGTTAACTGATGGAGA+TGG 0.666346 3:+83766480 MsaT015196.1:CDS
CGCCGATCCGAAAACCGATG+AGG 0.668414 3:+83764537 MsaT015196.1:CDS
ACCGTTGTTCGCATCAGACG+GGG 0.668514 3:-83764674 None:intergenic
GTGGTCAACAAATGCCGGCG+CGG 0.669526 3:-83764838 None:intergenic
AAGACACAATCTCGACACAT+CGG 0.674917 3:-83765010 None:intergenic
CACAAACCTACTCCAACCGG+TGG 0.675418 3:-83764857 None:intergenic
CGGCGGAAATGGCAGCACAG+GGG 0.678622 3:+83765142 MsaT015196.1:CDS
TGAACCCTGTGATAGAAAAG+GGG 0.678709 3:-83766506 None:intergenic
AGTGTTGCAAACTTCCAAGT+GGG 0.678749 3:+83766371 MsaT015196.1:CDS
CCGTCTGATGCGAACAACGG+TGG 0.680837 3:+83764676 MsaT015196.1:CDS
CTGTGGAGTGAGAAGTAGGG+TGG 0.681702 3:+83765038 MsaT015196.1:CDS
CCCATTGAAAAGGACTAGAG+GGG 0.682404 3:+83766452 MsaT015196.1:CDS
TGTATTATCCGAGAGCGGGG+TGG 0.682628 3:+83765181 MsaT015196.1:CDS
AGTGGTGTATTATCCGAGAG+CGG 0.685012 3:+83765176 MsaT015196.1:CDS
AAGTGTTGCAAACTTCCAAG+TGG 0.685015 3:+83766370 MsaT015196.1:CDS
AAACCCTAGAATTGACGGAG+GGG 0.688106 3:-83764402 None:intergenic
GGATCCATACTATAATCCAA+CGG 0.690465 3:-83764742 None:intergenic
GAACTAATCGAACAGCACGA+CGG 0.693501 3:-83764954 None:intergenic
CATACATGAAGCATACGCCA+AGG 0.694470 3:-83765375 None:intergenic
ATCGGCGAGGAAATGAACGG+CGG 0.707383 3:-83764521 None:intergenic
CCGGTGATTGAAGCTGACGA+CGG 0.709175 3:+83764619 MsaT015196.1:CDS
TCCATACTATAATCCAACGG+AGG 0.710511 3:-83764739 None:intergenic
GTAAACCCTAGAATTGACGG+AGG 0.732924 3:-83764404 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 83764310 83767055 83764310 ID=MsaG015196
Chr3 mRNA 83764310 83767055 83764310 ID=MsaT015196.1;Parent=MsaG015196
Chr3 exon 83764310 83765392 83764310 ID=MsaT015196.1.exon1;Parent=MsaT015196.1
Chr3 CDS 83764310 83765392 83764310 ID=cds.MsaT015196.1;Parent=MsaT015196.1
Chr3 exon 83766351 83767055 83766351 ID=MsaT015196.1.exon2;Parent=MsaT015196.1
Chr3 CDS 83766351 83767055 83766351 ID=cds.MsaT015196.1;Parent=MsaT015196.1
Gene Sequence

>MsaT015196.1

ATGAACCGCCAAGTACTACCACCGTCTCCAAAAAACGCAACCACACTCAACCCCAAACTCTGGCGAGCTATCGCCGGCGCTGCCGTGCAAATCCCCTCCGTCAATTCTAGGGTTTACTACTTCCCTCAAGGTCACATGGACCAAGCTACTTCACTTCCGAACAATCTCTCACCTCTTCTTCTCTCTCGTCCCTACATTCTCTGCTCCGTCTCCGCCGTTCATTTCCTCGCCGATCCGAAAACCGATGAGGTTTTCGCTAAGCTTTTTCTTCAACCGTTGAATGATTTCACTGTTAATTTTCCACGGATTCCGGTGATTGAAGCTGACGACGGTGAGAGGATTTCTTCGTTTGCGAAGATTCTTACCCCGTCTGATGCGAACAACGGTGGTGGATTTTCTGTTCCGAGGTTTTGTGCTGATTCGATTTTTCCTCCGTTGGATTATAGTATGGATCCTCCGTTGCAGAATTTGTTGATTACTGATGTTCATGGTTTGACTTGGGAGTTTCGTCACATTTACCGTGGAACGCCGCGCCGGCATTTGTTGACCACCGGTTGGAGTAGGTTTGTGAACGCTAAGAAGCTTGTTGCTGGTGATTCCGTTGTTTTTATGAAGAATACTAGAGGTGCAATGTTCATCGGAATCCGTCGTGCTGTTCGATTAGTTCCGAATCGGAGTAGCGCCGGTGGCGGTGTTTGCTCCGATGTGTCGAGATTGTGTCTTCCGATCTGTGGAGTGAGAAGTAGGGTGGATGAAGAAGAGAAGCTGGTGGATGAGAAGGCGTTTTCGAGGCACGGGAAGGGGAAGTTGTCTCCGGTGGCGGTTGCGGAGGCGGCGGAAATGGCAGCACAGGGGATGGGGTTTGAAGTGGTGTATTATCCGAGAGCGGGGTGGTCGGATTTTGTGTTGAAAGCGGAGGTTGTGGATGCGGCGATGAGTGTTACTTGGTGTCCTGGAATGAGAATCAAAATGGCTGTGGAGACTGATGATTCTTCCAGGACTACTTGGTTTCAAGGTGTAGTGTCTCAGGTTTCTGTTCCTGATCGTGGGGCTTGGAGAGGTTCACCTTGGCGTATGCTTCATATTACATGGGACGAACCTGAAGTGTTGCAAACTTCCAAGTGGGTCAGCCCTTGGCAGGTGGAACTTCTTTCTACTACACCTTCCCTTCATACGCCCTTTCCCCCATTGAAAAGGACTAGAGGGGTATCTGGGGTGTTAACTGATGGAGATGGAGACCCCTTTTCTATCACAGGGTTCACTAATTCAACAATGGGACAATTGAATCAACCATTGTTGAGTTACAGTACTTTTCCTGCTGGCATGCAGGGAGCCAGGCATGATCTATTTTCCGTTTCAAGTTTTTCCAACTTTCCAGGTGATAACTTCCGTCTTTGTATGGGTAACTCCTTTGGCAGCAACACAGTGCCGGGGTTAAAATCTTTTTCAACGGATCTAAACGTTGGCAGTTCTCACTCTGGTGATTTTTCACCAGAGAGCCAGAGTAGTTTGCCTTCCTTTGGCACGGATTTTGTTCGGAACTACAACTGCAACTCAATGAAACCCGGGCCTGTAACTTTTCAGCTATTTGGTGCAGTCATTCAGACAGAACAGCCCGTTGAAAGTGGTTCAAATGGTACTGTTAGCACAGGAGATGACAGCAGTAAGGGCTGCAACGAAACTGAAGGCATTAACAACCCTCTTGAGGATTCTACTTACTCAAAATTGCTCGACAGGCTTGATGGCCAGTGCCAAATAGCCTCTACAGTTGAGGCGTGCTATCTGTGA
Protein sequence

>MsaT015196.1

MNRQVLPPSPKNATTLNPKLWRAIAGAAVQIPSVNSRVYYFPQGHMDQATSLPNNLSPLLLSRPYILCSVSAVHFLADPKTDEVFAKLFLQPLNDFTVNFPRIPVIEADDGERISSFAKILTPSDANNGGGFSVPRFCADSIFPPLDYSMDPPLQNLLITDVHGLTWEFRHIYRGTPRRHLLTTGWSRFVNAKKLVAGDSVVFMKNTRGAMFIGIRRAVRLVPNRSSAGGGVCSDVSRLCLPICGVRSRVDEEEKLVDEKAFSRHGKGKLSPVAVAEAAEMAAQGMGFEVVYYPRAGWSDFVLKAEVVDAAMSVTWCPGMRIKMAVETDDSSRTTWFQGVVSQVSVPDRGAWRGSPWRMLHITWDEPEVLQTSKWVSPWQVELLSTTPSLHTPFPPLKRTRGVSGVLTDGDGDPFSITGFTNSTMGQLNQPLLSYSTFPAGMQGARHDLFSVSSFSNFPGDNFRLCMGNSFGSNTVPGLKSFSTDLNVGSSHSGDFSPESQSSLPSFGTDFVRNYNCNSMKPGPVTFQLFGAVIQTEQPVESGSNGTVSTGDDSSKGCNETEGINNPLEDSTYSKLLDRLDGQCQIASTVEACYL*