Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015287 | XP_024635817.1 | 71.717 | 495 | 91 | 7 | 1 | 480 | 111 | 571 | 0.0 | 618 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015287 | tr|A0A1S3E9P8|A0A1S3E9P8_CICAR | 50.800 | 500 | 175 | 11 | 1 | 480 | 1 | 449 | 1.17e-133 | 403 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG015287 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG003934 | MsaG015287 | 0.818914 | 2.669377e-52 | 5.705355e-50 |
| MsaG005598 | MsaG015287 | 0.807701 | 7.558370e-50 | 1.221209e-47 |
| MsaG007663 | MsaG015287 | 0.804001 | 4.489917e-49 | 6.651142e-47 |
| MsaG009864 | MsaG015287 | 0.801378 | 1.551878e-48 | 2.164567e-46 |
| MsaG012458 | MsaG015287 | 0.802108 | 1.101195e-48 | 1.561580e-46 |
| MsaG015287 | MsaG016688 | 0.813479 | 4.321793e-51 | 8.041040e-49 |
| MsaG015287 | MsaG017905 | 0.809209 | 3.614327e-50 | 6.055290e-48 |
| MsaG015287 | MsaG017958 | 0.801748 | 1.304744e-48 | 1.835196e-46 |
| MsaG015287 | MsaG018538 | 0.806272 | 1.510527e-49 | 2.359427e-47 |
| MsaG015287 | MsaG018860 | 0.800632 | 2.200912e-48 | 3.018639e-46 |
| MsaG015287 | MsaG019218 | 0.802908 | 7.547490e-49 | 1.090109e-46 |
| MsaG015287 | MsaG019740 | 0.809743 | 2.779271e-50 | 4.716918e-48 |
| MsaG015287 | MsaG020529 | 0.816219 | 1.074172e-51 | 2.141663e-49 |
| MsaG015287 | MsaG020894 | 0.844355 | 1.489043e-58 | 6.679670e-56 |
| MsaG015287 | MsaG022461 | 0.834617 | 4.918349e-56 | 1.628713e-53 |
| MsaG015287 | MsaG027285 | 0.819881 | 1.610709e-52 | 3.531259e-50 |
| MsaG015287 | MsaG027969 | 0.804498 | 3.542109e-49 | 5.307854e-47 |
| MsaG015287 | MsaG028813 | 0.831174 | 3.494589e-55 | 1.045942e-52 |
| MsaG015287 | MsaG028950 | 0.813555 | 4.158036e-51 | 7.751362e-49 |
| MsaG015287 | MsaG029743 | 0.811214 | 1.342518e-50 | 2.361585e-48 |
| MsaG015287 | MsaG032814 | 0.831065 | 3.716239e-55 | 1.108805e-52 |
| MsaG015287 | MsaG045265 | 0.833974 | 7.116058e-56 | 2.311588e-53 |
| MsaG015287 | MsaG046804 | 0.804179 | 4.125495e-49 | 6.136532e-47 |
| MsaG015287 | MsaG019459 | 0.823182 | 2.806035e-53 | 6.718024e-51 |
| MsaG015287 | MsaG041722 | 0.816413 | 9.724072e-52 | 1.948339e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015287 | MtrunA17_Chr3g0116411 | 73.500 | 200 | 40 | 4 | 1 | 197 | 111 | 300 | 1.40e-69 | 225 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 79 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATTGCAAATGAACAAAT+TGG | 0.173912 | 3:+85105669 | None:intergenic |
| GCCAAAGCCAAATGTTATTA+AGG | 0.179207 | 3:-85106121 | MsaT015287.1:CDS |
| GAAACAACTTCTACGCTATT+TGG | 0.184746 | 3:+85108278 | None:intergenic |
| AACTCTCCCTTAATAACATT+TGG | 0.298918 | 3:+85106114 | None:intergenic |
| AAGATGGTAATGTAGATAGA+AGG | 0.318676 | 3:-85108253 | MsaT015287.1:CDS |
| TCGGCTCACAAGCTTGAAAA+AGG | 0.326977 | 3:-85108476 | MsaT015287.1:CDS |
| ACTTGACGTGCCAATGCATA+TGG | 0.351764 | 3:-85108321 | MsaT015287.1:CDS |
| CCCTTAATAACATTTGGCTT+TGG | 0.353606 | 3:+85106120 | None:intergenic |
| AATTTCCTCTTTGAACATGA+TGG | 0.372502 | 3:+85106676 | None:intergenic |
| AGCCAAGTCACCGAGAGTTT+TGG | 0.375722 | 3:-85108056 | MsaT015287.1:intron |
| TGTTACAGCCCATCTCTTAC+CGG | 0.383658 | 3:+85108553 | None:intergenic |
| CAATGAGATGACAAATGATA+TGG | 0.387382 | 3:-85106460 | MsaT015287.1:CDS |
| AATGAAAATTTGAATGCAAC+AGG | 0.392051 | 3:-85105652 | MsaT015287.1:intron |
| CCAAAGCCAAATGTTATTAA+GGG | 0.399077 | 3:-85106120 | MsaT015287.1:CDS |
| CGTAATCCAGCGCGAGATAG+TGG | 0.405348 | 3:+85105146 | None:intergenic |
| TACCGAAGCCTTCAGAAAAC+TGG | 0.412567 | 3:+85106645 | None:intergenic |
| TCATTGCAAATGAACAAATT+GGG | 0.414322 | 3:+85105670 | None:intergenic |
| TTTGAAGAAGTGTTGGGCTC+AGG | 0.414789 | 3:-85108140 | MsaT015287.1:CDS |
| AGCCCATCTCTTACCGGTTG+AGG | 0.418637 | 3:+85108559 | None:intergenic |
| CTCATACGCTTCTAAATCGT+TGG | 0.425403 | 3:-85108111 | MsaT015287.1:CDS |
| ATCTGAGTCATGATTTACTT+TGG | 0.428076 | 3:+85106808 | None:intergenic |
| ATAGCAAATAATATTAAATC+AGG | 0.430447 | 3:-85105020 | MsaT015287.1:CDS |
| CTCGAGGATTCAACCTCAAC+CGG | 0.431548 | 3:-85108572 | MsaT015287.1:CDS |
| CAACGATTTAGAAGCGTATG+AGG | 0.431571 | 3:+85108112 | None:intergenic |
| AAATCCTACCATGTCATTGT+TGG | 0.435100 | 3:-85108612 | None:intergenic |
| ACTTTGATCATCTGAGAGAT+TGG | 0.438569 | 3:+85105819 | None:intergenic |
| GTAGAAGTTGTTTCAGAAGA+TGG | 0.439449 | 3:-85108269 | MsaT015287.1:CDS |
| AATTTCATTGAACCTCTGTT+TGG | 0.446936 | 3:+85105843 | None:intergenic |
| TTGTCAACGAAGTTTGTTCA+AGG | 0.455984 | 3:+85105063 | None:intergenic |
| ATTTCAGAAGTCATGTTCAT+AGG | 0.463203 | 3:+85106282 | None:intergenic |
| GAGGATGGTGGTGATACTTA+AGG | 0.478584 | 3:-85105117 | MsaT015287.1:CDS |
| CTTGACGTGCCAATGCATAT+GGG | 0.487224 | 3:-85108320 | MsaT015287.1:CDS |
| TCTTTCTTAATAATAATCTC+TGG | 0.487369 | 3:+85105543 | None:intergenic |
| GAAACTTGAAGAAAATAAAG+AGG | 0.495114 | 3:-85106705 | MsaT015287.1:CDS |
| TACCAAAACTCTCGGTGACT+TGG | 0.498675 | 3:+85108054 | None:intergenic |
| GAACACCTCACAGGAGATAG+TGG | 0.505357 | 3:+85106056 | None:intergenic |
| ATGATAAGTCTATGTGTGAA+AGG | 0.505569 | 3:-85108400 | MsaT015287.1:CDS |
| AGATGGTAATGTAGATAGAA+GGG | 0.509570 | 3:-85108252 | MsaT015287.1:CDS |
| AAATTGGGAGTCATTTCCAT+TGG | 0.525358 | 3:+85105685 | None:intergenic |
| CAACCTCAACCGGTAAGAGA+TGG | 0.526023 | 3:-85108562 | MsaT015287.1:CDS |
| TTGAAGAAGTGTTGGGCTCA+GGG | 0.526257 | 3:-85108139 | MsaT015287.1:CDS |
| TCCTATGTTTGAAGAAGTGT+TGG | 0.533675 | 3:-85108147 | MsaT015287.1:CDS |
| ACGAAGTTTGTTCAAGGCAA+AGG | 0.538459 | 3:+85105069 | None:intergenic |
| TTGACGTGCCAATGCATATG+GGG | 0.554500 | 3:-85108319 | MsaT015287.1:CDS |
| AACCTCAACCGGTAAGAGAT+GGG | 0.557870 | 3:-85108561 | MsaT015287.1:CDS |
| GAACAAACTTCGTTGACAAG+TGG | 0.558965 | 3:-85105059 | MsaT015287.1:CDS |
| CCTACCATGTCATTGTTGGT+TGG | 0.564330 | 3:-85108608 | None:intergenic |
| GGTGATACTTAAGGATGCAC+TGG | 0.567579 | 3:-85105108 | MsaT015287.1:CDS |
| AAGAGAAGTAGATGCAGCAG+TGG | 0.569759 | 3:-85108347 | MsaT015287.1:CDS |
| TGCACTGGAGAGATTGTGGC+CGG | 0.570410 | 3:-85105093 | MsaT015287.1:CDS |
| GTTTGTTCAAGGCAAAGGAC+CGG | 0.572102 | 3:+85105074 | None:intergenic |
| AATTATGGTACCAAAACTCT+CGG | 0.575030 | 3:+85108046 | None:intergenic |
| AGGATGCACTGGAGAGATTG+TGG | 0.575956 | 3:-85105097 | MsaT015287.1:CDS |
| TGAAAAGAAGAAAATGCCAA+TGG | 0.577507 | 3:-85105701 | MsaT015287.1:CDS |
| AAACTTGAAGAAAATAAAGA+GGG | 0.577987 | 3:-85106704 | MsaT015287.1:CDS |
| TGGATTACGTTCAAAGAGGA+TGG | 0.586400 | 3:-85105132 | MsaT015287.1:CDS |
| CCCAACACTTCTTCAAACAT+AGG | 0.592689 | 3:+85108146 | None:intergenic |
| ATGCATCATTCAGATCTACA+TGG | 0.593325 | 3:+85108186 | None:intergenic |
| TGCTTGCCACTATCTCGCGC+TGG | 0.598241 | 3:-85105152 | MsaT015287.1:CDS |
| CCTATGTTTGAAGAAGTGTT+GGG | 0.601870 | 3:-85108146 | MsaT015287.1:CDS |
| TGATAAGTCTATGTGTGAAA+GGG | 0.602703 | 3:-85108399 | MsaT015287.1:CDS |
| TTCTTAATAATAATCTCTGG+CGG | 0.603010 | 3:+85105546 | None:intergenic |
| GTAACAGTATCAAAAGCTGA+TGG | 0.606610 | 3:-85108536 | MsaT015287.1:CDS |
| CTTACCAACCAACAATGACA+TGG | 0.609549 | 3:+85108604 | None:intergenic |
| ATTATGAATGAACACCTCAC+AGG | 0.618032 | 3:+85106047 | None:intergenic |
| AATGATAAATTGAATGAACC+AGG | 0.618698 | 3:-85106249 | MsaT015287.1:intron |
| GACTCTACTAACATCACTTG+TGG | 0.623454 | 3:+85104937 | None:intergenic |
| TCAGATGATCAAAGTCACAT+TGG | 0.632225 | 3:-85105811 | MsaT015287.1:intron |
| TGAAGAAGTGTTGGGCTCAG+GGG | 0.642137 | 3:-85108138 | MsaT015287.1:CDS |
| CAGATCCATCATGTTCAAAG+AGG | 0.644431 | 3:-85106681 | MsaT015287.1:CDS |
| AAACTTCGTTGACAAGTGGA+TGG | 0.647156 | 3:-85105055 | MsaT015287.1:CDS |
| TGGTAAGAAAATCTATCTCG+AGG | 0.647585 | 3:-85108588 | MsaT015287.1:CDS |
| GCGCTGGATTACGTTCAAAG+AGG | 0.684747 | 3:-85105136 | MsaT015287.1:CDS |
| ATGGATGCACGAATGCACAT+TGG | 0.687374 | 3:-85106441 | MsaT015287.1:intron |
| ATTACGTTCAAAGAGGATGG+TGG | 0.691182 | 3:-85105129 | MsaT015287.1:CDS |
| TGCAGCCACTATCTCCTGTG+AGG | 0.708925 | 3:-85106061 | MsaT015287.1:intron |
| AGATGAAACTGTGAGCTCTG+TGG | 0.734871 | 3:-85105511 | MsaT015287.1:CDS |
| TGACGTGCCAATGCATATGG+GGG | 0.743356 | 3:-85108318 | MsaT015287.1:CDS |
| AACATGACTTCTGAAATGCA+CGG | 0.757981 | 3:-85106276 | MsaT015287.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 85104950 | 85108624 | 85104950 | ID=MsaG015287 |
| Chr3 | mRNA | 85104950 | 85108624 | 85104950 | ID=MsaT015287.1;Parent=MsaG015287 |
| Chr3 | exon | 85104950 | 85105186 | 85104950 | ID=MsaT015287.1.exon9;Parent=MsaT015287.1 |
| Chr3 | CDS | 85104950 | 85105186 | 85104950 | ID=cds.MsaT015287.1;Parent=MsaT015287.1 |
| Chr3 | exon | 85105488 | 85105570 | 85105488 | ID=MsaT015287.1.exon8;Parent=MsaT015287.1 |
| Chr3 | CDS | 85105488 | 85105570 | 85105488 | ID=cds.MsaT015287.1;Parent=MsaT015287.1 |
| Chr3 | exon | 85105653 | 85105724 | 85105653 | ID=MsaT015287.1.exon7;Parent=MsaT015287.1 |
| Chr3 | CDS | 85105653 | 85105724 | 85105653 | ID=cds.MsaT015287.1;Parent=MsaT015287.1 |
| Chr3 | exon | 85105812 | 85105869 | 85105812 | ID=MsaT015287.1.exon6;Parent=MsaT015287.1 |
| Chr3 | CDS | 85105812 | 85105869 | 85105812 | ID=cds.MsaT015287.1;Parent=MsaT015287.1 |
| Chr3 | exon | 85106062 | 85106147 | 85106062 | ID=MsaT015287.1.exon5;Parent=MsaT015287.1 |
| Chr3 | CDS | 85106062 | 85106147 | 85106062 | ID=cds.MsaT015287.1;Parent=MsaT015287.1 |
| Chr3 | exon | 85106250 | 85106321 | 85106250 | ID=MsaT015287.1.exon4;Parent=MsaT015287.1 |
| Chr3 | CDS | 85106250 | 85106321 | 85106250 | ID=cds.MsaT015287.1;Parent=MsaT015287.1 |
| Chr3 | exon | 85106442 | 85106504 | 85106442 | ID=MsaT015287.1.exon3;Parent=MsaT015287.1 |
| Chr3 | CDS | 85106442 | 85106504 | 85106442 | ID=cds.MsaT015287.1;Parent=MsaT015287.1 |
| Chr3 | exon | 85106648 | 85106851 | 85106648 | ID=MsaT015287.1.exon2;Parent=MsaT015287.1 |
| Chr3 | CDS | 85106648 | 85106851 | 85106648 | ID=cds.MsaT015287.1;Parent=MsaT015287.1 |
| Chr3 | exon | 85108057 | 85108624 | 85108057 | ID=MsaT015287.1.exon1;Parent=MsaT015287.1 |
| Chr3 | CDS | 85108057 | 85108624 | 85108057 | ID=cds.MsaT015287.1;Parent=MsaT015287.1 |
| Gene Sequence |
| Protein sequence |
