AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015364


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015364 MtrunA17_Chr3g0117121 100.000 191 0 0 1 191 1 191 9.55e-142 392
MsaG015364 MtrunA17_Chr4g0058651 75.000 176 34 4 7 177 8 178 3.53e-86 252
MsaG015364 MtrunA17_Chr7g0227511 59.278 194 56 3 1 193 1 172 1.68e-71 214
MsaG015364 MtrunA17_Chr6g0449641 64.151 159 30 2 1 159 1 132 1.01e-65 198
MsaG015364 MtrunA17_Chr6g0449651 78.261 115 25 0 1 115 58 172 9.28e-64 195
MsaG015364 MtrunA17_Chr8g0353981 75.221 113 25 1 1 113 1 110 3.03e-52 167
MsaG015364 MtrunA17_Chr5g0402611 55.556 153 58 1 4 156 2 144 1.80e-51 167
MsaG015364 MtrunA17_Chr5g0437571 71.154 104 30 0 8 111 4 107 2.73e-50 160
MsaG015364 MtrunA17_Chr2g0311601 56.934 137 59 0 9 145 12 148 4.52e-50 159
MsaG015364 MtrunA17_Chr3g0115431 54.140 157 68 2 4 157 3 158 8.84e-48 155
MsaG015364 MtrunA17_Chr4g0043421 65.714 105 36 0 9 113 14 118 2.18e-47 153
MsaG015364 MtrunA17_Chr3g0097221 55.245 143 64 0 5 147 2 144 6.40e-47 155
MsaG015364 MtrunA17_Chr5g0437401 53.571 140 54 1 8 136 9 148 7.15e-47 152
MsaG015364 MtrunA17_Chr3g0106101 64.423 104 37 0 8 111 5 108 2.05e-46 150
MsaG015364 MtrunA17_Chr3g0114061 66.346 104 35 0 8 111 6 109 6.56e-46 149
MsaG015364 MtrunA17_Chr5g0438091 51.408 142 60 2 8 140 37 178 6.40e-44 145
MsaG015364 MtrunA17_Chr3g0129071 60.550 109 43 0 9 117 46 154 1.57e-43 145
MsaG015364 MtrunA17_Chr3g0113891 65.625 96 33 0 8 103 12 107 1.80e-42 140
MsaG015364 MtrunA17_Chr4g0030001 55.455 110 48 1 9 117 23 132 8.42e-40 134
MsaG015364 MtrunA17_Chr1g0200201 58.252 103 43 0 11 113 73 175 3.37e-39 134
MsaG015364 MtrunA17_Chr5g0432451 57.692 130 45 2 9 128 42 171 3.81e-39 134
MsaG015364 MtrunA17_Chr7g0260971 57.798 109 45 1 3 110 7 115 2.27e-38 131
MsaG015364 MtrunA17_Chr8g0351701 57.143 105 44 1 10 113 23 127 2.00e-37 129
MsaG015364 MtrunA17_Chr8g0351721 56.410 117 50 1 10 125 18 134 8.14e-37 127
MsaG015364 MtrunA17_Chr1g0184281 54.701 117 47 2 1 111 1 117 6.43e-36 124
MsaG015364 MtrunA17_Chr7g0225111 50.909 110 51 1 9 118 4 110 1.48e-34 118
MsaG015364 MtrunA17_Chr7g0245871 61.053 95 36 1 10 103 27 121 1.65e-34 122
MsaG015364 MtrunA17_Chr6g0460951 51.818 110 50 2 9 115 39 148 1.67e-33 119
MsaG015364 MtrunA17_Chr5g0435641 51.485 101 48 1 5 104 2 102 2.96e-32 115
MsaG015364 MtrunA17_Chr7g0261031 57.292 96 40 1 9 103 7 102 4.65e-32 115
MsaG015364 MtrunA17_Chr8g0374601 46.789 109 58 0 10 118 26 134 6.23e-32 116
MsaG015364 MtrunA17_Chr1g0184271 54.737 95 42 1 10 103 12 106 1.20e-31 114
MsaG015364 MtrunA17_Chr5g0444351 44.628 121 65 1 11 129 6 126 1.42e-30 109
MsaG015364 MtrunA17_Chr8g0374591 47.115 104 55 0 10 113 8 111 1.63e-30 108
MsaG015364 MtrunA17_Chr7g0245861 47.458 118 59 2 10 124 12 129 9.22e-30 107
MsaG015364 MtrunA17_Chr4g0059901 48.000 100 52 0 11 110 11 110 1.88e-29 109
MsaG015364 MtrunA17_Chr8g0374581 46.364 110 58 1 10 118 8 117 4.04e-28 105
MsaG015364 MtrunA17_Chr6g0452661 45.918 98 52 1 8 104 7 104 7.15e-28 105
MsaG015364 MtrunA17_Chr8g0342351 43.689 103 58 0 11 113 13 115 1.92e-26 101
MsaG015364 MtrunA17_Chr6g0460941 48.315 89 45 1 10 97 7 95 5.70e-26 95.9
MsaG015364 MtrunA17_Chr6g0452671 35.252 139 85 2 11 145 12 149 3.11e-25 97.1
MsaG015364 MtrunA17_Chr6g0452691 39.370 127 72 2 8 129 7 133 4.91e-24 93.6
MsaG015364 MtrunA17_Chr4g0055441 41.053 95 55 1 11 104 3 97 2.47e-23 90.9
MsaG015364 MtrunA17_Chr2g0330201 43.011 93 53 0 11 103 20 112 4.33e-23 93.6
MsaG015364 MtrunA17_Chr4g0040831 30.488 164 103 3 1 161 6 161 3.02e-22 89.4
MsaG015364 MtrunA17_Chr5g0439111 39.423 104 62 1 8 110 7 110 6.01e-20 84.0
MsaG015364 MtrunA17_Chr2g0326041 60.784 51 20 0 9 59 32 82 8.64e-19 77.0
MsaG015364 MtrunA17_Chr4g0046201 29.878 164 98 4 9 163 5 160 2.40e-18 80.5
MsaG015364 MtrunA17_Chr2g0326051 55.932 59 26 0 55 113 5 63 3.38e-16 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015364 AT5G63090.4 75.000 148 19 2 1 130 1 148 3.55e-73 219
MsaG015364 AT5G63090.3 75.000 148 19 2 1 130 1 148 3.55e-73 219
MsaG015364 AT5G63090.2 75.000 148 19 2 1 130 1 148 3.55e-73 219
MsaG015364 AT5G63090.1 75.000 148 19 2 1 130 1 148 3.55e-73 219
MsaG015364 AT3G27650.1 79.279 111 23 0 4 114 33 143 4.19e-63 192
MsaG015364 AT1G65620.1 73.451 113 29 1 1 113 1 112 3.95e-51 164
MsaG015364 AT1G65620.4 73.451 113 29 1 1 113 1 112 3.95e-51 164
MsaG015364 AT1G65620.3 73.451 113 29 1 1 113 1 112 3.95e-51 164
MsaG015364 AT1G65620.5 73.451 113 29 1 1 113 1 112 3.95e-51 164
MsaG015364 AT1G65620.2 73.451 113 29 1 1 113 1 112 3.95e-51 164
MsaG015364 AT5G66870.1 62.791 129 41 2 1 129 1 122 2.17e-50 165
MsaG015364 AT2G23660.2 60.504 119 43 1 8 126 3 117 7.37e-49 161
MsaG015364 AT2G23660.3 60.504 119 43 1 8 126 3 117 7.37e-49 161
MsaG015364 AT2G23660.1 60.504 119 43 1 8 126 3 117 7.37e-49 161
MsaG015364 AT2G30130.1 69.231 104 32 0 8 111 6 109 8.45e-49 157
MsaG015364 AT2G30340.2 57.759 116 49 0 9 124 52 167 4.48e-44 147
MsaG015364 AT2G30340.1 57.759 116 49 0 9 124 51 166 4.51e-44 147
MsaG015364 AT1G31320.1 72.727 88 24 0 9 96 12 99 1.42e-43 143
MsaG015364 AT1G07900.1 58.333 108 45 0 9 116 32 139 2.42e-43 143
MsaG015364 AT1G16530.1 62.264 106 39 1 9 113 13 118 1.36e-42 140
MsaG015364 AT2G28500.1 60.194 103 41 0 9 111 54 156 2.47e-41 139
MsaG015364 AT3G11090.1 54.167 120 52 2 8 127 9 125 1.58e-39 133
MsaG015364 AT3G26660.1 56.000 100 44 0 11 110 6 105 2.83e-38 128
MsaG015364 AT3G26620.1 56.000 100 44 0 11 110 6 105 3.05e-38 128
MsaG015364 AT2G40470.2 53.676 136 50 2 9 139 19 146 3.72e-38 130
MsaG015364 AT2G40470.1 53.676 136 50 2 9 139 44 171 7.48e-38 130
MsaG015364 AT3G03760.1 55.769 104 45 1 9 111 50 153 3.61e-36 127
MsaG015364 AT2G45420.1 59.596 99 39 1 13 110 40 138 2.96e-35 125
MsaG015364 AT4G00220.1 59.596 99 39 1 13 110 20 118 3.79e-35 124
MsaG015364 AT5G06080.1 42.405 158 87 3 5 161 2 156 6.13e-34 119
MsaG015364 AT2G42430.1 55.340 103 45 1 9 110 14 116 1.09e-33 120
MsaG015364 AT2G45410.1 55.085 118 47 3 13 126 19 134 2.67e-33 117
MsaG015364 AT4G00210.1 49.600 125 54 2 3 126 4 120 2.67e-33 119
MsaG015364 AT2G31310.1 55.208 96 42 1 9 103 6 101 1.19e-32 116
MsaG015364 AT3G58190.1 55.208 96 42 1 10 104 11 106 2.00e-32 116
MsaG015364 AT2G42440.1 52.577 97 45 1 9 104 6 102 8.29e-32 115
MsaG015364 AT5G35900.1 46.610 118 63 0 11 128 6 123 3.28e-31 113
MsaG015364 AT3G50510.1 44.915 118 62 1 9 126 11 125 4.70e-30 109
MsaG015364 AT3G50510.2 44.915 118 62 1 9 126 11 125 4.70e-30 109
MsaG015364 AT3G13850.1 44.660 103 57 0 11 113 37 139 1.55e-28 107
MsaG015364 AT3G47870.1 42.000 100 58 0 11 110 37 136 9.28e-25 99.0
MsaG015364 AT1G72980.1 44.792 96 53 0 9 104 12 107 8.75e-23 91.3
MsaG015364 AT4G22700.1 37.984 129 76 3 11 136 6 133 2.42e-21 87.0
MsaG015364 AT1G06280.1 42.500 80 46 0 8 87 22 101 5.44e-18 78.6
MsaG015364 AT5G15060.1 40.260 77 44 1 7 81 14 90 5.53e-15 68.9
MsaG015364 AT2G19820.1 36.667 90 56 1 9 97 11 100 5.93e-15 68.6
MsaG015364 AT3G27940.3 39.394 66 40 0 10 75 5 70 2.45e-11 59.7
MsaG015364 AT3G27940.1 39.394 66 40 0 10 75 5 70 2.45e-11 59.7
MsaG015364 AT3G27940.2 39.394 66 40 0 10 75 5 70 2.45e-11 59.7

Find 49 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGGTGGAAGAGAGGAATT+AGG 0.310902 3:-85959366 None:intergenic
AGACAGTTTAGTTCAAGATT+TGG 0.321989 3:+85959429 MsaT015364.1:CDS
TCTCCCTCCTCTTGTTGTTC+CGG 0.367027 3:+85959386 MsaT015364.1:CDS
GGTTCTTCTGGTGGAAAGTA+TGG 0.385993 3:-85959093 None:intergenic
TTTGGTAATGGAAATGTTGA+TGG 0.387117 3:+85959447 MsaT015364.1:CDS
AATGGAAATGTTGATGGAAA+TGG 0.392662 3:+85959453 MsaT015364.1:CDS
GGAGGAAGAGGAGGAGGTGT+AGG 0.401282 3:+85959549 MsaT015364.1:CDS
GGTGTAGGAGGAAGAGGAGG+AGG 0.413675 3:+85959543 MsaT015364.1:CDS
GGAAGAGGAGGAGGTGTAGG+AGG 0.430574 3:+85959552 MsaT015364.1:CDS
AGTGGAGGTGTAGGAGGAAG+AGG 0.435749 3:+85959537 MsaT015364.1:CDS
GATATAAGTGGAGGTGTAGG+AGG 0.445011 3:+85959531 MsaT015364.1:CDS
AGGAGGGAGAGGTGGAAGAG+AGG 0.445135 3:-85959373 None:intergenic
AGAGGAGGAGGTGTAGGAGG+AGG 0.445819 3:+85959555 MsaT015364.1:CDS
TTTAGTTCAAGATTTGGTAA+TGG 0.446442 3:+85959435 MsaT015364.1:CDS
CGTGTGAGAGATCCTGTTTA+TGG 0.449251 3:+85959237 MsaT015364.1:CDS
AATTCACTGCATCTTCTCTC+TGG 0.451599 3:-85959191 None:intergenic
TTAATCCATGGAATAGCATA+AGG 0.469520 3:-85959492 None:intergenic
GCACCAACACAACCATAAAC+AGG 0.472967 3:-85959249 None:intergenic
GAGGATATAAGTGGAGGTGT+AGG 0.488241 3:+85959528 MsaT015364.1:CDS
GCCAACGTTCACAAGATCTT+CGG 0.491748 3:+85959126 MsaT015364.1:CDS
CTGCATCTTCTCTCTGGTGA+GGG 0.502538 3:-85959185 None:intergenic
CTTCGTCGAAAATGCATGCC+AGG 0.503647 3:+85959057 MsaT015364.1:CDS
ACAAGTTCAGAGGCTTCAAA+AGG 0.506762 3:+85959290 MsaT015364.1:CDS
GCCTCTGAACTTGTCTCTGA+AGG 0.518925 3:-85959281 None:intergenic
GATCCTGTTTATGGTTGTGT+TGG 0.519160 3:+85959246 MsaT015364.1:CDS
GGAGGTGTAGGAGGAAGAGG+AGG 0.524745 3:+85959540 MsaT015364.1:CDS
ACCTCTTCTGAGGATATAAG+TGG 0.541161 3:+85959519 MsaT015364.1:CDS
ACAACAAGAGGAGGGAGAGG+TGG 0.543814 3:-85959381 None:intergenic
CATCTTCTCTCTGGTGAGGG+AGG 0.545055 3:-85959182 None:intergenic
AGGAATTTGCAGGCTGCACA+TGG 0.547297 3:-85959036 None:intergenic
ACTGCATCTTCTCTCTGGTG+AGG 0.556115 3:-85959186 None:intergenic
TGAGATTGATGAAACGAAGC+CGG 0.556305 3:-85959405 None:intergenic
GGAACAACAAGAGGAGGGAG+AGG 0.557580 3:-85959384 None:intergenic
TCCACTTATATCCTCAGAAG+AGG 0.570244 3:-85959520 None:intergenic
TCCTTCAGAGACAAGTTCAG+AGG 0.577279 3:+85959280 MsaT015364.1:CDS
GATTAATGATACCTCTTCTG+AGG 0.582743 3:+85959509 MsaT015364.1:CDS
AACGAAGCCGGAACAACAAG+AGG 0.585009 3:-85959393 None:intergenic
TATGGTGCAAAGATGCAGCC+TGG 0.588900 3:-85959075 None:intergenic
TTCCCTTGCTTATGAAGCCG+AGG 0.588970 3:+85959212 MsaT015364.1:CDS
AGGATCTCTCACACGTGCCT+CGG 0.589439 3:-85959229 None:intergenic
GAAGAGGTATCATTAATCCA+TGG 0.590052 3:-85959504 None:intergenic
GTGCCTCGGCTTCATAAGCA+AGG 0.591344 3:-85959215 None:intergenic
GAAGCCGGAACAACAAGAGG+AGG 0.593525 3:-85959390 None:intergenic
GCCGAAGATCTTGTGAACGT+TGG 0.617944 3:-85959127 None:intergenic
TTGTCTCTGAAGGAAAGAGA+TGG 0.626573 3:-85959271 None:intergenic
AAGCCGGAACAACAAGAGGA+GGG 0.639232 3:-85959389 None:intergenic
TGCCTCGGCTTCATAAGCAA+GGG 0.648971 3:-85959214 None:intergenic
GGTGAGGGAGGAGTTCGTTG+AGG 0.665155 3:-85959170 None:intergenic
TCTTCTGAGGATATAAGTGG+AGG 0.670110 3:+85959522 MsaT015364.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 85959009 85959590 85959009 ID=MsaG015364
Chr3 mRNA 85959009 85959590 85959009 ID=MsaT015364.1;Parent=MsaG015364
Chr3 exon 85959009 85959590 85959009 ID=MsaT015364.1.exon1;Parent=MsaT015364.1
Chr3 CDS 85959009 85959590 85959009 ID=cds.MsaT015364.1;Parent=MsaT015364.1
Gene Sequence

>MsaT015364.1

ATGGCATCATCAAGCTCATACAATTCACCATGTGCAGCCTGCAAATTCCTTCGTCGAAAATGCATGCCAGGCTGCATCTTTGCACCATACTTTCCACCAGAAGAACCTCAAAAATTCGCCAACGTTCACAAGATCTTCGGCGCAAGCAACGTCACGAAGATCCTCAACGAACTCCTCCCTCACCAGAGAGAAGATGCAGTGAATTCCCTTGCTTATGAAGCCGAGGCACGTGTGAGAGATCCTGTTTATGGTTGTGTTGGTGCCATCTCTTTCCTTCAGAGACAAGTTCAGAGGCTTCAAAAGGAACTTGATTCTGCTAATGCTGATCTTCTTCGCTTTGCTTACAATGAAATCTCTCCTAATTCCTCTCTTCCACCTCTCCCTCCTCTTGTTGTTCCGGCTTCGTTTCATCAATCTCAAAGACAGTTTAGTTCAAGATTTGGTAATGGAAATGTTGATGGAAATGGATTTTACTCTTCTTTTCCTTATGCTATTCCATGGATTAATGATACCTCTTCTGAGGATATAAGTGGAGGTGTAGGAGGAAGAGGAGGAGGTGTAGGAGGAGGTAATAATTTGTGA

Protein sequence

>MsaT015364.1

MASSSSYNSPCAACKFLRRKCMPGCIFAPYFPPEEPQKFANVHKIFGASNVTKILNELLPHQREDAVNSLAYEAEARVRDPVYGCVGAISFLQRQVQRLQKELDSANADLLRFAYNEISPNSSLPPLPPLVVPASFHQSQRQFSSRFGNGNVDGNGFYSSFPYAIPWINDTSSEDISGGVGGRGGGVGGGNNL*