AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015371


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015371 MtrunA17_Chr3g0117261 93.542 480 31 0 15 494 16 495 0.0 939
MsaG015371 MtrunA17_Chr7g0268031 28.165 316 148 14 7 303 197 452 1.17e-19 92.0
MsaG015371 MtrunA17_Chr3g0132281 25.000 296 165 13 12 299 132 378 3.93e-15 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015371 AT5G63080.1 56.356 472 180 6 8 476 3 451 0.0 533
MsaG015371 AT5G06550.1 25.000 324 157 13 14 319 194 449 3.24e-14 75.5
MsaG015371 AT1G78280.1 23.411 299 166 10 12 299 125 371 5.34e-13 72.0

Find 89 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAACTTATGCAGACTATT+TGG 0.218231 3:+86075419 MsaT015371.1:intron
GTATCATCAAGTTCATAATT+TGG 0.246758 3:+86075492 MsaT015371.1:CDS
CACTTGTTCTGGCAGGATCT+TGG 0.254470 3:+86074807 MsaT015371.1:intron
GTGCTGCTTTGCTATATATA+TGG 0.271036 3:+86076352 MsaT015371.1:CDS
CTCAAAATCAGAGGACAAAT+TGG 0.271585 3:-86076593 None:intergenic
TCAATGCCTATAACCTTTCT+TGG 0.293305 3:+86075719 MsaT015371.1:CDS
ACTGATTGGGTTACACCTAA+TGG 0.293546 3:+86073417 MsaT015371.1:CDS
AGTGGTGTTAACTGGATTAA+TGG 0.294120 3:+86073374 MsaT015371.1:CDS
GTTATGTATGCTACATATTC+TGG 0.294875 3:-86074540 None:intergenic
CATCAACAACATCAAAATAA+AGG 0.300767 3:+86073275 MsaT015371.1:CDS
TTTGTTTATATGGGAGTTAA+AGG 0.310574 3:+86074675 MsaT015371.1:CDS
AACAAACCAGTGGTGTTAAC+TGG 0.321042 3:+86073366 MsaT015371.1:CDS
TGAATTTGTTGATAGATATA+TGG 0.323277 3:+86073338 MsaT015371.1:CDS
CTTTAAATATTGCGTCTATT+AGG 0.325382 3:+86076425 MsaT015371.1:CDS
ATAAATTTGATGAGGTCTTC+AGG 0.341372 3:-86076685 None:intergenic
GATGTCTTCAATGTATTCCT+TGG 0.357631 3:-86075879 None:intergenic
GTTCAGTGCAGTAATGAAAA+TGG 0.363737 3:+86073875 MsaT015371.1:CDS
CTGGATTAATGGATCATCAT+TGG 0.366707 3:+86073385 MsaT015371.1:CDS
GAGTGCTTCATTGGTTGATT+TGG 0.369774 3:+86076615 MsaT015371.1:CDS
TACCACCCAAGAAAGGTTAT+AGG 0.377980 3:-86075725 None:intergenic
ATTGGAGAGCTTCCACTGAT+TGG 0.381303 3:+86073403 MsaT015371.1:CDS
CAATGCCTATAACCTTTCTT+GGG 0.382210 3:+86075720 MsaT015371.1:CDS
TGACAGTGACCAGCAAAATA+AGG 0.385417 3:+86074629 MsaT015371.1:CDS
AGACAATACCGTCGCAGAGC+TGG 0.401710 3:+86076708 MsaT015371.1:CDS
AAACACGTGCGTGCTCTAGA+AGG 0.404523 3:+86076466 MsaT015371.1:CDS
TTGGAGAGCTTCCACTGATT+GGG 0.421960 3:+86073404 MsaT015371.1:CDS
ATCAACAACATCAAAATAAA+GGG 0.427273 3:+86073276 MsaT015371.1:CDS
CTGCAGAACCCTCAACTTCA+AGG 0.428740 3:-86073853 None:intergenic
TTTCATTTCATACTTTGCCT+TGG 0.431384 3:+86076321 MsaT015371.1:CDS
CTGTACCGTATTTGAAGGAT+TGG 0.436080 3:+86073921 MsaT015371.1:CDS
GAGAAATTGAGAAGGTGAAC+GGG 0.436671 3:+86073298 MsaT015371.1:CDS
CAATATCTATAAACCACAAT+TGG 0.441545 3:+86075695 MsaT015371.1:CDS
CAGCAAGGTTGCGCTGGCAG+AGG 0.448536 3:-86075928 None:intergenic
TTGGTTGATTTGGTGGGACT+CGG 0.453555 3:+86076625 MsaT015371.1:CDS
CATGCCTGTCAAACACGAAA+TGG 0.455876 3:-86074922 None:intergenic
CTCAATTTACCACCCAAGAA+AGG 0.458313 3:-86075732 None:intergenic
ATACTTTGCCTTGGCCAACT+TGG 0.465254 3:+86076330 MsaT015371.1:CDS
TGTGTCTGTACCGTATTTGA+AGG 0.465358 3:+86073916 MsaT015371.1:CDS
AGTGCCATTTCGTGTTTGAC+AGG 0.469316 3:+86074918 MsaT015371.1:CDS
TGCTTCATTGGTTGATTTGG+TGG 0.472657 3:+86076618 MsaT015371.1:CDS
TGAACGTTGCCTTGAAGTTG+AGG 0.484615 3:+86073844 MsaT015371.1:CDS
GACAATACCGTCGCAGAGCT+GGG 0.485971 3:+86076709 MsaT015371.1:CDS
TTCTAAATTCTGCATGCAGG+TGG 0.497798 3:+86076540 MsaT015371.1:CDS
GCTTCATTGGTTGATTTGGT+GGG 0.500949 3:+86076619 MsaT015371.1:CDS
AATGAAAATGGTTCAAGTAA+TGG 0.504999 3:+86073887 MsaT015371.1:CDS
CGCAACCTTGCTGCCAACAC+AGG 0.505053 3:+86075938 MsaT015371.1:CDS
AAATGCCAATCCTTCAAATA+CGG 0.505177 3:-86073926 None:intergenic
TGTTGGCAGCAAGGTTGCGC+TGG 0.509302 3:-86075934 None:intergenic
TGTCAAACACGAAATGGCAC+TGG 0.510078 3:-86074916 None:intergenic
GAACGTTGCCTTGAAGTTGA+GGG 0.513815 3:+86073845 MsaT015371.1:CDS
TTAGAATGCACACAAGAAGC+AGG 0.515116 3:+86075442 MsaT015371.1:CDS
ATACAGATGACTCCATGTTA+AGG 0.518181 3:-86076735 None:intergenic
TATAGGCATTGAACCAATTG+TGG 0.520435 3:-86075708 None:intergenic
TCATATTTGTTCCCAGTGGA+TGG 0.526700 3:+86075470 MsaT015371.1:CDS
ATTAATCCAGTTAACACCAC+TGG 0.527498 3:-86073372 None:intergenic
AACTTGATGATACCATCCAC+TGG 0.531578 3:-86075482 None:intergenic
GGAATACATTGAAGACATCA+AGG 0.541622 3:+86075883 MsaT015371.1:CDS
AAATATTGCGTCTATTAGGA+AGG 0.550115 3:+86076429 MsaT015371.1:CDS
AAGCAGCACCAAGTTGGCCA+AGG 0.555336 3:-86076338 None:intergenic
TACTCTTGACAAACTTTCAG+TGG 0.558191 3:-86076389 None:intergenic
GTCAAACACGAAATGGCACT+GGG 0.569616 3:-86074915 None:intergenic
TGCCTATAACCTTTCTTGGG+TGG 0.577403 3:+86075723 MsaT015371.1:CDS
ATGTCTGTGGAAAGAAGAGA+TGG 0.577930 3:+86074876 MsaT015371.1:CDS
GGAGAAATTGAGAAGGTGAA+CGG 0.580825 3:+86073297 MsaT015371.1:CDS
TCTAAATTCTGCATGCAGGT+GGG 0.581693 3:+86076541 MsaT015371.1:CDS
AACACGTGCGTGCTCTAGAA+GGG 0.582042 3:+86076467 MsaT015371.1:CDS
CTGCACCTGTGTTGGCAGCA+AGG 0.594405 3:-86075943 None:intergenic
AACACGAAATGGCACTGGGA+AGG 0.599724 3:-86074911 None:intergenic
GAGCGACTACAATGAGGCCA+AGG 0.603057 3:+86075862 MsaT015371.1:CDS
TAGAATGCACACAAGAAGCA+GGG 0.604713 3:+86075443 MsaT015371.1:CDS
AATTGAGAAGGTGAACGGGA+AGG 0.605761 3:+86073302 MsaT015371.1:CDS
AAGGTCGCCCAGCTCTGCGA+CGG 0.606124 3:-86076716 None:intergenic
ACACAGTACTGCACCTGTGT+TGG 0.611154 3:-86075951 None:intergenic
AGAGCTGGGCGACCTTAACA+TGG 0.618534 3:+86076723 MsaT015371.1:CDS
ATAGCAAAGCAGCACCAAGT+TGG 0.619753 3:-86076344 None:intergenic
ATTAGGTGTAACCCAATCAG+TGG 0.622628 3:-86073415 None:intergenic
AGCTGGTCAGCAAATGTCTG+TGG 0.623034 3:+86074863 MsaT015371.1:CDS
TATTGTCTATAAATTTGATG+AGG 0.630189 3:-86076693 None:intergenic
ATGCAGAATTTAGAAAACCT+AGG 0.631755 3:-86076532 None:intergenic
CTGAAAACATCAGCATGTAG+AGG 0.641625 3:-86074833 None:intergenic
TGAAGCACTCTCAAAATCAG+AGG 0.642152 3:-86076602 None:intergenic
GTATCTGACTCAAAATACCC+TGG 0.643979 3:+86075311 MsaT015371.1:CDS
AATAAAGGGAGAAATTGAGA+AGG 0.646492 3:+86073290 MsaT015371.1:CDS
ACTTGATGATACCATCCACT+GGG 0.651707 3:-86075481 None:intergenic
AGAATGCACACAAGAAGCAG+GGG 0.665619 3:+86075444 MsaT015371.1:CDS
GAAATCATATTTGTTCCCAG+TGG 0.671903 3:+86075466 MsaT015371.1:CDS
CAATCATCACAAAAGTACGT+TGG 0.708793 3:-86074564 None:intergenic
TATGGACAAGAACAAACCAG+TGG 0.727874 3:+86073356 MsaT015371.1:CDS
AGGGACTCATGATTGCATCG+TGG 0.769193 3:+86076486 MsaT015371.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 86073264 86076771 86073264 ID=MsaG015371
Chr3 mRNA 86073264 86076771 86073264 ID=MsaT015371.1;Parent=MsaG015371
Chr3 exon 86073264 86073491 86073264 ID=MsaT015371.1.exon1;Parent=MsaT015371.1
Chr3 CDS 86073264 86073491 86073264 ID=cds.MsaT015371.1;Parent=MsaT015371.1
Chr3 exon 86073776 86073955 86073776 ID=MsaT015371.1.exon2;Parent=MsaT015371.1
Chr3 CDS 86073776 86073955 86073776 ID=cds.MsaT015371.1;Parent=MsaT015371.1
Chr3 exon 86074534 86074696 86074534 ID=MsaT015371.1.exon3;Parent=MsaT015371.1
Chr3 CDS 86074534 86074696 86074534 ID=cds.MsaT015371.1;Parent=MsaT015371.1
Chr3 exon 86074822 86074939 86074822 ID=MsaT015371.1.exon4;Parent=MsaT015371.1
Chr3 CDS 86074822 86074939 86074822 ID=cds.MsaT015371.1;Parent=MsaT015371.1
Chr3 exon 86075274 86075343 86075274 ID=MsaT015371.1.exon5;Parent=MsaT015371.1
Chr3 CDS 86075274 86075343 86075274 ID=cds.MsaT015371.1;Parent=MsaT015371.1
Chr3 exon 86075433 86075513 86075433 ID=MsaT015371.1.exon6;Parent=MsaT015371.1
Chr3 CDS 86075433 86075513 86075433 ID=cds.MsaT015371.1;Parent=MsaT015371.1
Chr3 exon 86075688 86075744 86075688 ID=MsaT015371.1.exon7;Parent=MsaT015371.1
Chr3 CDS 86075688 86075744 86075688 ID=cds.MsaT015371.1;Parent=MsaT015371.1
Chr3 exon 86075848 86075959 86075848 ID=MsaT015371.1.exon8;Parent=MsaT015371.1
Chr3 CDS 86075848 86075959 86075848 ID=cds.MsaT015371.1;Parent=MsaT015371.1
Chr3 exon 86076296 86076771 86076296 ID=MsaT015371.1.exon9;Parent=MsaT015371.1
Chr3 CDS 86076296 86076771 86076296 ID=cds.MsaT015371.1;Parent=MsaT015371.1
Gene Sequence

>MsaT015371.1

ATGGGTATCATCATCAACAACATCAAAATAAAGGGAGAAATTGAGAAGGTGAACGGGAAGGAATTGAGTTACAGTGAATTTGTTGATAGATATATGGACAAGAACAAACCAGTGGTGTTAACTGGATTAATGGATCATCATTGGAGAGCTTCCACTGATTGGGTTACACCTAATGGAAAACCTAATCTTCAATTTTTCTTCAATCATTTTGGTGCTTCCAAAGTTCAGGTTGCAGATTGTGATACTAGAGATTTCACTGATCAAAAAAGAGAGGAGATGTTGGTCTCTGATTTCGTTGAACGTTGCCTTGAAGTTGAGGGTTCTGCAGTTCAGTGCAGTAATGAAAATGGTTCAAGTAATGGTGACTCTGTGTCTGTACCGTATTTGAAGGATTGGCATTTTGTCAAGGAGTATCCAGAATATGTAGCATACATAACTCCAACGTACTTTTGTGATGATTGGCTTAACTTGTATCTTGACAACTTCAGAATGAATACTAGTTCTGACAGTGACCAGCAAAATAAGGAAATCTGCTGCTCTGACTATCGTTTTGTTTATATGGGAGTTAAAGGATCTTGGACTCCTCTACATGCTGATGTTTTCAGGTCGTACAGCTGGTCAGCAAATGTCTGTGGAAAGAAGAGATGGTTTTTTCTAGATCCTTCCCAGTGCCATTTCGTGTTTGACAGGAATGCGAAAAGCTCTGTATATAATATCTTTGATGAAGTATCTGACTCAAAATACCCTGGTTTTGAAAAGACTATTTGGTTAGAATGCACACAAGAAGCAGGGGAAATCATATTTGTTCCCAGTGGATGGTATCATCAAGTTCATAATTTGGAGGATACAATATCTATAAACCACAATTGGTTCAATGCCTATAACCTTTCTTGGGTGTGGAATTTACTTTTGAGCGACTACAATGAGGCCAAGGAATACATTGAAGACATCAAGGATATATGTGATGATTTTGAAGGCCTCTGCCAGCGCAACCTTGCTGCCAACACAGGGATGAATTTTTTTGACTTCTTTACTTTCATTTCATACTTTGCCTTGGCCAACTTGGTGCTGCTTTGCTATATATATGGAAGAGATAGAAGCACCACTGAAAGTTTGTCAAGAGTAGCTCAGCATTTATCTTTAAATATTGCGTCTATTAGGAAGGTTGCATCAGATATGAAACACGTGCGTGCTCTAGAAGGGACTCATGATTGCATCGTGGATATGAGAAAAACATTTGAAGATCCTAGGTTTTCTAAATTCTGCATGCAGGTGGGAAAAGCATATTTAATGATACATGAGCAATCCAATTTGTCCTCTGATTTTGAGAGTGCTTCATTGGTTGATTTGGTGGGACTCGGTGTTTTGAAAGCGTGCACTTCTTCTCAGATTTACACACCTGAAGACCTCATCAAATTTATAGACAATACCGTCGCAGAGCTGGGCGACCTTAACATGGAGTCATCTGTATTGCACGTTGCTTGA

Protein sequence

>MsaT015371.1

MGIIINNIKIKGEIEKVNGKELSYSEFVDRYMDKNKPVVLTGLMDHHWRASTDWVTPNGKPNLQFFFNHFGASKVQVADCDTRDFTDQKREEMLVSDFVERCLEVEGSAVQCSNENGSSNGDSVSVPYLKDWHFVKEYPEYVAYITPTYFCDDWLNLYLDNFRMNTSSDSDQQNKEICCSDYRFVYMGVKGSWTPLHADVFRSYSWSANVCGKKRWFFLDPSQCHFVFDRNAKSSVYNIFDEVSDSKYPGFEKTIWLECTQEAGEIIFVPSGWYHQVHNLEDTISINHNWFNAYNLSWVWNLLLSDYNEAKEYIEDIKDICDDFEGLCQRNLAANTGMNFFDFFTFISYFALANLVLLCYIYGRDRSTTESLSRVAQHLSLNIASIRKVASDMKHVRALEGTHDCIVDMRKTFEDPRFSKFCMQVGKAYLMIHEQSNLSSDFESASLVDLVGLGVLKACTSSQIYTPEDLIKFIDNTVAELGDLNMESSVLHVA*