AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015432


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015432 MtrunA17_Chr3g0118211 97.500 200 5 0 1 200 1 200 2.02e-140 390
MsaG015432 MtrunA17_Chr4g0059571 68.837 215 51 5 1 200 1 214 2.95e-89 261
MsaG015432 MtrunA17_Chr1g0170741 61.688 154 53 2 22 172 7 157 3.30e-60 186
MsaG015432 MtrunA17_Chr5g0414931 65.493 142 45 1 17 154 10 151 2.46e-59 186
MsaG015432 MtrunA17_Chr7g0256551 53.086 162 67 2 18 170 4 165 1.76e-54 173
MsaG015432 MtrunA17_Chr3g0082861 52.147 163 69 2 20 173 1 163 3.52e-52 165
MsaG015432 MtrunA17_Chr8g0352871 55.034 149 57 2 18 157 4 151 1.19e-50 161
MsaG015432 MtrunA17_Chr3g0128691 59.091 132 50 2 24 152 19 149 4.87e-50 159
MsaG015432 MtrunA17_Chr3g0124861 51.678 149 53 2 22 152 21 168 5.91e-47 154
MsaG015432 MtrunA17_Chr4g0021731 54.651 86 39 0 15 100 3 88 1.57e-25 94.7
MsaG015432 MtrunA17_Chr3g0127941 29.054 148 96 2 23 170 56 194 3.53e-15 73.2
MsaG015432 MtrunA17_Chr8g0345901 28.906 128 82 1 25 152 38 156 2.16e-13 68.2
MsaG015432 MtrunA17_Chr4g0030271 30.827 133 83 1 20 152 86 209 3.16e-13 67.8
MsaG015432 MtrunA17_Chr1g0149851 28.662 157 97 4 23 179 9 150 6.93e-12 63.5
MsaG015432 MtrunA17_Chr1g0181811 29.323 133 85 2 20 152 82 205 1.09e-11 63.2
MsaG015432 MtrunA17_Chr7g0275401 29.054 148 86 3 23 170 9 137 3.51e-11 60.8
MsaG015432 MtrunA17_Chr7g0276361 25.385 130 88 1 23 152 14 134 4.91e-11 61.2
MsaG015432 MtrunA17_Chr7g0275891 30.405 148 85 4 23 170 9 138 6.48e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015432 AT3G48100.1 64.088 181 59 2 5 179 2 182 3.30e-77 229
MsaG015432 AT1G74890.1 75.839 149 30 2 22 166 17 163 1.99e-73 221
MsaG015432 AT5G62920.1 75.000 144 35 1 23 166 25 167 2.44e-72 217
MsaG015432 AT1G19050.1 67.516 157 49 1 3 157 2 158 4.34e-71 215
MsaG015432 AT1G59940.1 63.218 174 45 4 1 157 1 172 3.03e-68 208
MsaG015432 AT1G59940.2 63.218 174 45 4 1 157 93 264 8.56e-67 208
MsaG015432 AT1G10470.3 66.892 148 49 0 14 161 11 158 3.01e-65 201
MsaG015432 AT1G10470.1 73.282 131 35 0 22 152 33 163 7.29e-65 201
MsaG015432 AT3G57040.2 57.931 145 51 1 18 152 4 148 5.69e-55 175
MsaG015432 AT3G57040.1 57.931 145 51 1 18 152 4 148 5.69e-55 175
MsaG015432 AT2G41310.1 57.047 149 56 2 21 161 7 155 5.89e-55 174
MsaG015432 AT2G40670.1 59.259 135 50 2 22 152 28 161 4.42e-52 165
MsaG015432 AT3G56380.1 58.333 132 54 1 22 152 19 150 8.55e-52 164
MsaG015432 AT2G40670.2 58.824 136 50 3 22 152 28 162 2.80e-50 160
MsaG015432 AT3G56380.2 57.895 133 54 2 22 152 19 151 5.08e-50 159
MsaG015432 AT1G10470.2 66.129 62 21 0 100 161 35 96 5.69e-21 86.3
MsaG015432 AT2G07440.1 57.377 61 26 0 101 161 9 69 1.17e-15 70.9
MsaG015432 AT5G07210.2 29.545 176 99 6 1 174 1 153 8.25e-14 69.7
MsaG015432 AT5G24470.1 30.000 130 82 2 23 152 50 170 9.53e-14 69.3
MsaG015432 AT2G46790.3 27.692 130 85 1 23 152 37 157 6.88e-13 67.0
MsaG015432 AT2G46790.1 27.692 130 85 1 23 152 37 157 8.61e-13 66.6
MsaG015432 AT5G60100.7 31.579 133 82 2 20 152 61 184 3.07e-12 64.7
MsaG015432 AT5G60100.6 31.579 133 82 2 20 152 61 184 3.07e-12 64.7
MsaG015432 AT5G60100.4 31.579 133 82 2 20 152 61 184 3.07e-12 64.7
MsaG015432 AT5G60100.2 31.579 133 82 2 20 152 61 184 3.20e-12 65.1
MsaG015432 AT5G60100.5 31.579 133 82 2 20 152 61 184 4.82e-12 64.3
MsaG015432 AT5G60100.3 31.579 133 82 2 20 152 61 184 4.82e-12 64.3
MsaG015432 AT5G60100.1 31.579 133 82 2 20 152 61 184 4.82e-12 64.3
MsaG015432 AT1G67710.1 29.655 145 90 3 14 158 2 134 2.17e-11 62.4
MsaG015432 AT5G07210.1 28.261 184 99 7 1 174 1 161 2.66e-11 62.4

Find 41 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGATCTTACCTATCAATT+CGG 0.347926 3:-86862697 None:intergenic
TGAATTGCTCAAGAAGATTA+AGG 0.356380 3:+86862458 MsaT015432.1:CDS
ACTAGAGCTTTACAGTATCT+TGG 0.363036 3:+86862225 MsaT015432.1:CDS
AGCTTTACAGTATCTTGGTT+TGG 0.366392 3:+86862230 MsaT015432.1:CDS
CTACTGCTGGTGATGTTCTC+AGG 0.387823 3:+86861956 MsaT015432.1:CDS
TGTTCTCAGGCAGAGTTTAC+CGG 0.414947 3:+86861969 MsaT015432.1:CDS
TTCACATCTGCCAACTTGAC+AGG 0.430476 3:-86864007 None:intergenic
ATGAATCAATGGTTATAGTC+TGG 0.434730 3:-86864191 None:intergenic
ACATCTTGACCCGAATTGAT+AGG 0.448548 3:+86862687 MsaT015432.1:CDS
ATGACAGATTATTCTATGCC+TGG 0.452458 3:+86862423 MsaT015432.1:CDS
TATTCTATGCCTGGGATGAC+AGG 0.453139 3:+86862432 MsaT015432.1:CDS
CTTAAATTCACTTCTTGCAA+AGG 0.463478 3:+86862075 MsaT015432.1:CDS
ATTGACAGTTGCTTGGAAGA+AGG 0.475916 3:+86863962 MsaT015432.1:intron
ACACCAGCACCACTTACTTC+CGG 0.479536 3:-86861988 None:intergenic
TTACAGTATCTTGGTTTGGA+TGG 0.481557 3:+86862234 MsaT015432.1:CDS
GATGGTGACACGAGTTCCAT+TGG 0.497003 3:+86862252 MsaT015432.1:CDS
AAGATCTTACCTATCAATTC+GGG 0.507592 3:-86862696 None:intergenic
TTTGTTCATTGACAGTTGCT+TGG 0.513588 3:+86863955 MsaT015432.1:intron
AGAGGTGAAGGAAAGAAAGT+CGG 0.518178 3:+86864052 MsaT015432.1:CDS
TTTGCTTACATCAAAACCAA+TGG 0.528283 3:-86862268 None:intergenic
TTACCGGAAGTAAGTGGTGC+TGG 0.532656 3:+86861985 MsaT015432.1:CDS
GAAGTAAGTGGTGCTGGTGT+TGG 0.533059 3:+86861991 MsaT015432.1:CDS
TTGATCGCAAAGTCATTGAA+AGG 0.535022 3:+86862049 MsaT015432.1:CDS
GCAGTTACTGTTGTTGAGAG+TGG 0.535906 3:+86862201 MsaT015432.1:intron
AATTCATATCCTGTCATCCC+AGG 0.536075 3:-86862441 None:intergenic
TTGTATTTCATCTCTATCAA+CGG 0.543618 3:+86864111 MsaT015432.1:CDS
TGACAGATTATTCTATGCCT+GGG 0.547632 3:+86862424 MsaT015432.1:CDS
TCAGAGGACATAACCACCAC+TGG 0.556667 3:-86862662 None:intergenic
TCTATTGAAACCTGTCAAGT+TGG 0.561378 3:+86863997 MsaT015432.1:CDS
TTCTTTGACGACATTGCAGA+CGG 0.571495 3:-86864166 None:intergenic
GGTGAAGGAAAGAAAGTCGG+AGG 0.571633 3:+86864055 MsaT015432.1:CDS
AGAGTTGCACGTTCTTGCAG+TGG 0.580799 3:+86862014 MsaT015432.1:CDS
ATTTAGAGAAATTCCAGTGG+TGG 0.593101 3:+86862649 MsaT015432.1:CDS
GATTTCATAATGAGAGGTGA+AGG 0.593144 3:+86864040 MsaT015432.1:CDS
TTAACAGATTTCATAATGAG+AGG 0.593540 3:+86864034 MsaT015432.1:CDS
GACATTGCAGACGGTGACAA+TGG 0.595350 3:-86864157 None:intergenic
CAGAGTTTACCGGAAGTAAG+TGG 0.596307 3:+86861979 MsaT015432.1:CDS
TTGCTTGGAAGAAGGAGCAG+AGG 0.596704 3:+86863970 MsaT015432.1:CDS
AATATGGTTACAGGGTGTGA+AGG 0.609461 3:+86862389 MsaT015432.1:intron
TGTATTTAGAGAAATTCCAG+TGG 0.707997 3:+86862646 MsaT015432.1:CDS
TCGGGTCAAGATGTTCTCAG+AGG 0.723155 3:-86862678 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 86861952 86864201 86861952 ID=MsaG015432
Chr3 mRNA 86861952 86864201 86861952 ID=MsaT015432.1;Parent=MsaG015432
Chr3 exon 86861952 86862096 86861952 ID=MsaT015432.1.exon1;Parent=MsaT015432.1
Chr3 CDS 86861952 86862096 86861952 ID=cds.MsaT015432.1;Parent=MsaT015432.1
Chr3 exon 86862205 86862281 86862205 ID=MsaT015432.1.exon2;Parent=MsaT015432.1
Chr3 CDS 86862205 86862281 86862205 ID=cds.MsaT015432.1;Parent=MsaT015432.1
Chr3 exon 86862402 86862479 86862402 ID=MsaT015432.1.exon3;Parent=MsaT015432.1
Chr3 CDS 86862402 86862479 86862402 ID=cds.MsaT015432.1;Parent=MsaT015432.1
Chr3 exon 86862638 86862708 86862638 ID=MsaT015432.1.exon4;Parent=MsaT015432.1
Chr3 CDS 86862638 86862708 86862638 ID=cds.MsaT015432.1;Parent=MsaT015432.1
Chr3 exon 86863970 86864201 86863970 ID=MsaT015432.1.exon5;Parent=MsaT015432.1
Chr3 CDS 86863970 86864201 86863970 ID=cds.MsaT015432.1;Parent=MsaT015432.1
Gene Sequence

>MsaT015432.1

ATGGCTACTGCTGGTGATGTTCTCAGGCAGAGTTTACCGGAAGTAAGTGGTGCTGGTGTTGGAGAGTTGCACGTTCTTGCAGTGGATGATAGTTTTGTTGATCGCAAAGTCATTGAAAGGTTGCTTAAATTCACTTCTTGCAAAGTTACTGTTGTTGAGAGTGGAACTAGAGCTTTACAGTATCTTGGTTTGGATGGTGACACGAGTTCCATTGGTTTTGATGGTGTGAAGGTTAATATGATAATGACAGATTATTCTATGCCTGGGATGACAGGATATGAATTGCTCAAGAAGATTAAGGAATCATCTGTATTTAGAGAAATTCCAGTGGTGGTTATGTCCTCTGAGAACATCTTGACCCGAATTGATAGTTGCTTGGAAGAAGGAGCAGAGGAGTTTCTATTGAAACCTGTCAAGTTGGCAGATGTGAAACGCTTAACAGATTTCATAATGAGAGGTGAAGGAAAGAAAGTCGGAGGAAAAATATCTCAAAAAAGAAGTCGGTCAGATGATTGTATTTCATCTCTATCAACGGCTTGTTCAACATGTGATCTGTCGCCATTGTCACCGTCTGCAATGTCGTCAAAGAAATCCAGACTATAA

Protein sequence

>MsaT015432.1

MATAGDVLRQSLPEVSGAGVGELHVLAVDDSFVDRKVIERLLKFTSCKVTVVESGTRALQYLGLDGDTSSIGFDGVKVNMIMTDYSMPGMTGYELLKKIKESSVFREIPVVVMSSENILTRIDSCLEEGAEEFLLKPVKLADVKRLTDFIMRGEGKKVGGKISQKRSRSDDCISSLSTACSTCDLSPLSPSAMSSKKSRL*