AlfalfaGEDB | MsaG015517

M. sativa ssp. caerulea / MsaG015517

KEGG: K09338
GO: -
PFAM: PF00046.32

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG015517	XP_003601381.1	94.902	255	13	0	1	255	1	255	4.17e-176	494

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG015517	sp\|P46667\|ATHB5_ARATH	53.571	168	65	4	1	155	1	168	4.05e-48	164

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG015517	tr\|G7J6Q0\|G7J6Q0_MEDTR	94.902	255	13	0	1	255	1	255	1.99e-176	494

TFs/TRs:

Gene ID	Type	Classification
MsaG015517	TF	HB-HD-ZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000904	MsaG015517	0.801242	1.654659e-48	2.300726e-46
MsaG004443	MsaG015517	0.813019	5.446985e-51	1.001878e-48
MsaG004734	MsaG015517	0.810364	2.045692e-50	3.524698e-48
MsaG007204	MsaG015517	0.809571	3.025704e-50	5.113957e-48
MsaG011811	MsaG015517	0.800454	2.392677e-48	3.268357e-46
MsaG012081	MsaG015517	0.806031	1.697158e-49	2.635647e-47
MsaG015517	MsaG015466	0.934805	6.450821e-96	3.898445e-91
MsaG015517	MsaG017371	0.820512	1.156473e-52	2.578208e-50
MsaG015517	MsaG018676	0.809051	3.906629e-50	6.520470e-48
MsaG015517	MsaG019543	0.804840	3.007076e-49	4.542104e-47
MsaG015517	MsaG023150	0.806662	1.251262e-49	1.972438e-47
MsaG015517	MsaG024877	0.825838	6.696755e-54	1.724085e-51
MsaG015517	MsaG029306	0.805130	2.616955e-49	3.979519e-47
MsaG015517	MsaG035833	0.817651	5.141314e-52	1.063444e-49
MsaG015517	MsaG042772	0.814980	2.021726e-51	3.906179e-49
MsaG015517	MsaG044608	0.806188	1.573288e-49	2.452473e-47
MsaG015517	MsaG045052	0.819200	2.299467e-52	4.952013e-50
MsaG015517	MsaG045980	0.805356	2.347849e-49	3.589065e-47
MsaG015517	MsaG046255	0.801859	1.238189e-48	1.746024e-46
MsaG015517	MsaG013380	0.817664	5.107482e-52	1.056799e-49
MsaG015517	MsaG031490	0.822435	4.180808e-53	9.809427e-51
MsaG015517	MsaG030125	0.801276	1.628106e-48	2.265584e-46
MsaG015517	MsaG032389	0.823771	2.046614e-53	4.979009e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG015517	MtrunA17_Chr3g0119291	94.902	255	13	0	1	255	1	255	3.84e-180	494
MsaG015517	MtrunA17_Chr8g0381741	37.987	308	132	9	1	255	1	302	1.82e-54	177
MsaG015517	MtrunA17_Chr3g0123691	40.067	297	132	6	1	255	1	293	1.63e-51	170
MsaG015517	MtrunA17_Chr6g0453021	46.465	198	91	4	42	238	82	265	7.15e-41	141
MsaG015517	MtrunA17_Chr8g0362151	57.724	123	51	1	46	168	64	185	1.06e-38	136
MsaG015517	MtrunA17_Chr5g0416391	57.600	125	52	1	44	168	62	185	2.28e-38	135
MsaG015517	MtrunA17_Chr5g0415941	60.000	110	44	0	47	156	75	184	5.55e-37	132
MsaG015517	MtrunA17_Chr8g0362751	60.577	104	41	0	59	162	89	192	7.75e-35	126
MsaG015517	MtrunA17_Chr6g0450991	64.286	98	35	0	58	155	66	163	1.79e-34	125
MsaG015517	MtrunA17_Chr4g0060031	61.905	105	40	0	58	162	94	198	3.39e-34	125
MsaG015517	MtrunA17_Chr7g0217111	51.938	129	58	2	47	174	71	196	4.75e-33	121
MsaG015517	MtrunA17_Chr7g0265521	47.761	134	68	2	52	185	56	187	4.03e-30	112
MsaG015517	MtrunA17_Chr1g0179581	57.831	83	35	0	58	140	27	109	7.43e-26	97.8
MsaG015517	MtrunA17_Chr8g0346251	51.163	86	42	0	66	151	48	133	3.21e-24	97.4
MsaG015517	MtrunA17_Chr3g0127511	48.352	91	47	0	61	151	40	130	2.88e-23	94.7
MsaG015517	MtrunA17_Chr4g0054421	47.748	111	49	2	43	153	113	214	9.82e-21	88.6
MsaG015517	MtrunA17_Chr4g0055851	46.667	105	49	1	49	153	110	207	3.07e-20	87.4
MsaG015517	MtrunA17_Chr5g0403701	44.915	118	53	1	51	168	45	150	3.44e-20	85.9
MsaG015517	MtrunA17_Chr5g0403691	45.000	120	57	3	51	168	45	157	1.85e-19	84.0
MsaG015517	MtrunA17_Chr1g0175651	48.113	106	46	2	48	153	145	241	1.57e-18	82.8
MsaG015517	MtrunA17_Chr7g0258831	47.170	106	47	2	48	153	151	247	1.67e-18	82.8
MsaG015517	MtrunA17_Chr2g0308611	42.222	135	58	3	19	153	173	287	1.89e-18	83.2
MsaG015517	MtrunA17_Chr4g0071411	39.130	138	74	2	19	153	45	175	6.07e-18	80.1
MsaG015517	MtrunA17_Chr5g0399491	42.857	133	56	3	21	152	112	225	1.27e-17	80.5
MsaG015517	MtrunA17_Chr5g0400781	46.226	106	49	2	48	153	113	210	3.04e-17	79.0
MsaG015517	MtrunA17_Chr1g0151971	33.333	168	71	5	59	215	130	267	2.30e-16	76.6
MsaG015517	MtrunA17_Chr8g0335131	46.316	95	44	1	59	153	146	233	4.77e-16	76.3
MsaG015517	MtrunA17_Chr4g0044091	44.211	95	46	1	59	153	65	152	1.26e-14	70.5
MsaG015517	MtrunA17_Chr3g0135331	38.406	138	68	4	57	186	143	271	7.20e-14	70.1
MsaG015517	MtrunA17_Chr2g0299031	44.211	95	46	1	59	153	82	169	2.66e-13	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG015517	AT5G65310.1	53.571	168	65	4	1	155	1	168	4.11e-49	164
MsaG015517	AT5G65310.2	59.441	143	47	2	24	155	8	150	9.33e-49	163
MsaG015517	AT4G40060.1	35.065	308	123	9	1	255	1	284	1.77e-44	152
MsaG015517	AT2G22430.1	49.057	159	75	2	1	155	2	158	1.78e-42	147
MsaG015517	AT1G69780.1	60.163	123	48	1	46	168	72	193	2.17e-40	141
MsaG015517	AT5G15150.1	58.594	128	49	3	42	166	95	221	5.43e-39	138
MsaG015517	AT1G26960.1	55.303	132	58	1	41	172	53	183	3.33e-37	132
MsaG015517	AT3G01220.1	50.000	146	66	3	44	187	70	210	3.83e-35	127
MsaG015517	AT3G01470.1	60.000	100	40	0	56	155	65	164	3.30e-33	122
MsaG015517	AT3G61890.1	39.161	143	82	2	62	202	33	172	1.50e-27	106
MsaG015517	AT1G27045.1	39.766	171	89	3	57	224	66	225	4.11e-27	104
MsaG015517	AT1G27045.2	39.766	171	89	3	57	224	15	174	5.31e-27	103
MsaG015517	AT1G27045.5	39.766	171	89	3	57	224	23	182	5.51e-27	103
MsaG015517	AT1G27045.3	39.766	171	89	3	57	224	23	182	5.51e-27	103
MsaG015517	AT2G46680.1	50.538	93	46	0	60	152	33	125	2.61e-26	103
MsaG015517	AT1G27045.4	53.684	95	44	0	57	151	66	160	2.04e-25	100
MsaG015517	AT2G46680.2	48.958	96	48	1	60	154	33	128	6.09e-25	99.8
MsaG015517	AT2G18550.1	39.521	167	78	4	59	217	61	212	1.49e-22	92.8
MsaG015517	AT5G03790.1	50.000	92	46	0	59	150	77	168	9.91e-22	90.9
MsaG015517	AT4G36740.1	47.368	114	56	1	52	161	48	161	2.20e-21	89.4
MsaG015517	AT4G36740.2	47.368	114	56	1	52	161	49	162	2.42e-21	89.4
MsaG015517	AT5G03790.2	52.439	82	39	0	59	140	77	158	6.53e-21	87.8
MsaG015517	AT2G36610.1	45.545	101	47	1	58	150	70	170	1.02e-20	87.0
MsaG015517	AT5G66700.1	35.429	175	101	5	46	217	60	225	2.76e-20	86.7
MsaG015517	AT5G53980.1	42.268	97	56	0	59	155	11	107	4.09e-19	82.0
MsaG015517	AT2G01430.1	43.119	109	55	1	45	153	125	226	2.29e-18	82.4
MsaG015517	AT5G47370.1	42.424	132	63	2	21	152	101	219	3.71e-18	82.0
MsaG015517	AT5G06710.1	39.850	133	75	2	20	152	152	279	1.39e-17	81.3
MsaG015517	AT4G17460.1	42.063	126	62	3	32	153	104	222	1.42e-17	80.5
MsaG015517	AT4G37790.1	42.241	116	59	2	38	153	106	213	7.55e-17	78.6
MsaG015517	AT2G44910.1	46.316	95	44	1	59	153	163	250	6.17e-16	76.3
MsaG015517	AT2G22800.1	40.650	123	71	2	48	169	101	222	1.40e-15	74.7
MsaG015517	AT3G60390.1	42.593	108	58	1	45	152	148	251	2.66e-15	74.7
MsaG015517	AT4G16780.1	43.689	103	50	2	51	153	122	216	7.25e-15	72.8
MsaG015517	AT2G01430.2	43.529	85	44	1	45	125	125	209	1.02e-12	65.9
MsaG015517	AT1G70920.1	38.525	122	73	1	23	144	35	154	4.03e-12	63.9
MsaG015517	AT1G70920.2	38.525	122	73	1	23	144	5	124	4.14e-12	63.5
MsaG015517	AT5G06710.4	39.560	91	54	1	20	110	152	241	4.76e-12	64.7

Find 36 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCAAGTTCAATGATGAATT+TGG	0.158147	3:-87742488	MsaT015517.1:CDS
TCGTTCGTGCTCGACGATTT+TGG	0.160811	3:+87742886	None:intergenic
CTTTCTATACCTCTTGAAAT+TGG	0.202435	3:+87742592	None:intergenic
AACCTTAAGAACATCAAAAT+TGG	0.244715	3:+87742824	None:intergenic
TTGAAGTTAGCTGAAGAATT+AGG	0.251335	3:-87742939	MsaT015517.1:CDS
AACCTAGACAAGTTTCAATT+TGG	0.262579	3:-87742911	MsaT015517.1:CDS
TAATTCAAGTGGTGTGATTA+AGG	0.320954	3:-87742554	MsaT015517.1:CDS
ATAATCTCTTTCCAATTGTT+TGG	0.328373	3:+87742860	None:intergenic
TTTAGAAAAGAGCTTTGAAT+TGG	0.355867	3:-87742988	MsaT015517.1:CDS
GATTCTCCTATATGTTTCAA+TGG	0.381447	3:-87742517	MsaT015517.1:CDS
GTTCAATAACTCAGAAACTA+TGG	0.387793	3:-87743301	MsaT015517.1:CDS
TCAGAATCAATTTATGAGAA+TGG	0.408136	3:-87742425	MsaT015517.1:CDS
CATACCTTTGGATTGGTACA+TGG	0.415046	3:+87743266	None:intergenic
ACCAAATTCATCATTGAACT+TGG	0.453246	3:+87742487	None:intergenic
GTTCATTATTATGATTCAAA+AGG	0.461757	3:-87742466	MsaT015517.1:CDS
ACTTATACATACCTTTGGAT+TGG	0.465929	3:+87743259	None:intergenic
CTCAGTTGGTATTACAAAGT+AGG	0.474989	3:+87742343	None:intergenic
AAAGAACCATTGAAACATAT+AGG	0.475600	3:+87742511	None:intergenic
CTTTGAATTGGACAACAAGC+TGG	0.480175	3:-87742976	MsaT015517.1:CDS
TCAGTTGGTATTACAAAGTA+GGG	0.482035	3:+87742344	None:intergenic
ACGAACGAAAACCAAACAAT+TGG	0.484787	3:-87742871	MsaT015517.1:CDS
GAATGAGACTCTTACTAGAA+AGG	0.510168	3:-87742778	MsaT015517.1:intron
TGCAAATGCAGGTGTGGATG+TGG	0.525574	3:-87743147	MsaT015517.1:intron
CAAGAAGATAGCTATGAAGA+TGG	0.532967	3:-87743062	MsaT015517.1:CDS
TGAAGATGGTAGTTCAATGT+TGG	0.535009	3:-87743048	MsaT015517.1:CDS
AACCAAATTGAAACTTGTCT+AGG	0.545141	3:+87742909	None:intergenic
ATTTCAAGAGGTATAGAAAG+AGG	0.548836	3:-87742589	MsaT015517.1:CDS
GATCAGAGGAATGAGACAAA+AGG	0.552396	3:-87743125	MsaT015517.1:CDS
CTTTCCATGTACCAATCCAA+AGG	0.553684	3:-87743270	MsaT015517.1:intron
TTGGAGAAGAAAAGAAGACT+TGG	0.569876	3:-87743029	MsaT015517.1:CDS
GTAGAATGTCCAATTTCAAG+AGG	0.608484	3:-87742601	MsaT015517.1:CDS
GAATCAATTTATGAGAATGG+AGG	0.629666	3:-87742422	MsaT015517.1:CDS
TCAAGAGGTATAGAAAGAGG+AGG	0.641487	3:-87742586	MsaT015517.1:CDS
CAGTTGGTATTACAAAGTAG+GGG	0.656897	3:+87742345	None:intergenic
GGTTCAGAAAGTAATTCAAG+TGG	0.665455	3:-87742565	MsaT015517.1:CDS
CAGGTGTGGATGTGGATCAG+AGG	0.671722	3:-87743139	MsaT015517.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	87742354	87743331	87742354	ID=MsaG015517
Chr3	mRNA	87742354	87743331	87742354	ID=MsaT015517.1;Parent=MsaG015517
Chr3	exon	87742354	87742680	87742354	ID=MsaT015517.1.exon3;Parent=MsaT015517.1
Chr3	CDS	87742354	87742680	87742354	ID=cds.MsaT015517.1;Parent=MsaT015517.1
Chr3	exon	87742779	87743158	87742779	ID=MsaT015517.1.exon2;Parent=MsaT015517.1
Chr3	CDS	87742779	87743158	87742779	ID=cds.MsaT015517.1;Parent=MsaT015517.1
Chr3	exon	87743271	87743331	87743271	ID=MsaT015517.1.exon1;Parent=MsaT015517.1
Chr3	CDS	87743271	87743331	87743271	ID=cds.MsaT015517.1;Parent=MsaT015517.1

Gene Sequence

>MsaT015517.1

ATGAAGAGGTTCAATAACTCAGAAACTATGGCTTCTTTGCTTTCCATGTACCAATCCAAAGGTGTGGATGTGGATCAGAGGAATGAGACAAAAGGTTACAGTGAAGAATTTCAAGCAATGTTAGATAGATTAGAGCAAGAAGATAGCTATGAAGATGGTAGTTCAATGTTGGAGAAGAAAAGAAGACTTGGTTATGATCAAGTAAAAGCTTTAGAAAAGAGCTTTGAATTGGACAACAAGCTGGAGCCAGAGAGAAAATTGAAGTTAGCTGAAGAATTAGGCTTGCAACCTAGACAAGTTTCAATTTGGTTCCAAAATCGTCGAGCACGAACGAAAACCAAACAATTGGAAAGAGATTATAGTGTTCTTAAGTCCAATTTTGATGTTCTTAAGGTTGAGTATAGTACTCTTCAACAAGAGAATGAGACTCTTACTAGAAAGTTAAGAGAGTTGAAAGCGAAATTGTTAAGAGTTGAAAGCAGTGAGTTAGAGAAAGAAGTAGAATGTCCAATTTCAAGAGGTATAGAAAGAGGAGGTTCAGAAAGTAATTCAAGTGGTGTGATTAAGGAAGAAAGTAATGTTGATTCTCCTATATGTTTCAATGGTTCTTTGCCAAGTTCAATGATGAATTTGGTTCATTATTATGATTCAAAAGGTTCTCAAGCAAATGTGTTTCAGAATCAATTTATGAGAATGGAGGAGCAGAATTTCTTAAGTAATAGTGAATTATTCAGTTTCTTTTCAGTTGATCAATCCCCTACTTTGTAA

Protein sequence

>MsaT015517.1

MKRFNNSETMASLLSMYQSKGVDVDQRNETKGYSEEFQAMLDRLEQEDSYEDGSSMLEKKRRLGYDQVKALEKSFELDNKLEPERKLKLAEELGLQPRQVSIWFQNRRARTKTKQLERDYSVLKSNFDVLKVEYSTLQQENETLTRKLRELKAKLLRVESSELEKEVECPISRGIERGGSESNSSGVIKEESNVDSPICFNGSLPSSMMNLVHYYDSKGSQANVFQNQFMRMEEQNFLSNSELFSFFSVDQSPTL*