Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015576 | XP_024633849.1 | 86.885 | 122 | 7 | 2 | 1 | 113 | 1 | 122 | 2.08e-65 | 203 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015576 | tr|A0A396IWL4|A0A396IWL4_MEDTR | 86.885 | 122 | 7 | 2 | 1 | 113 | 1 | 122 | 9.93e-66 | 203 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG015576 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015576 | MtrunA17_Chr3g0120121 | 86.885 | 122 | 7 | 2 | 1 | 113 | 1 | 122 | 1.91e-69 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAAACGAAATTGTTGTT+TGG | 0.350812 | 3:-88585469 | MsaT015576.1:CDS |
| TGCAGGCTAGAGCGATAATA+TGG | 0.383290 | 3:-88584950 | MsaT015576.1:intron |
| ATGGTTGGCAAGTCAAGAAA+TGG | 0.426643 | 3:-88584931 | MsaT015576.1:CDS |
| CAAGAAATGGAAGGAAAGAA+TGG | 0.466725 | 3:-88584918 | MsaT015576.1:CDS |
| ATGCACTAGGTTGCATTGAA+AGG | 0.491779 | 3:+88584865 | None:intergenic |
| TGCACTAGGTTGCATTGAAA+GGG | 0.509370 | 3:+88584866 | None:intergenic |
| ACACCACAGATTTGACGAAA+AGG | 0.545745 | 3:-88585278 | MsaT015576.1:CDS |
| TGCATTGAAAGGGATAGTGT+TGG | 0.550068 | 3:+88584876 | None:intergenic |
| GGCTAGAGCGATAATATGGT+TGG | 0.554001 | 3:-88584946 | MsaT015576.1:CDS |
| TCCTTGAGAAGAGAAAGAAA+AGG | 0.554506 | 3:-88584791 | MsaT015576.1:CDS |
| TTACAAATGCATGCCTCACA+TGG | 0.582863 | 3:-88584843 | MsaT015576.1:CDS |
| CTTGTTGCAAATCCTCGCAA+TGG | 0.611244 | 3:-88585301 | MsaT015576.1:CDS |
| AATCTGTGGTGTCCATTGCG+AGG | 0.627615 | 3:+88585289 | None:intergenic |
| GCATGCATTTGTAATGCACT+AGG | 0.632578 | 3:+88584852 | None:intergenic |
| TTGGCAAGTCAAGAAATGGA+AGG | 0.668042 | 3:-88584927 | MsaT015576.1:CDS |
| CTTTATGGAAAAGCCATGTG+AGG | 0.679093 | 3:+88584830 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 88584752 | 88585490 | 88584752 | ID=MsaG015576 |
| Chr3 | mRNA | 88584752 | 88585490 | 88584752 | ID=MsaT015576.1;Parent=MsaG015576 |
| Chr3 | exon | 88584752 | 88584967 | 88584752 | ID=MsaT015576.1.exon3;Parent=MsaT015576.1 |
| Chr3 | CDS | 88584752 | 88584967 | 88584752 | ID=cds.MsaT015576.1;Parent=MsaT015576.1 |
| Chr3 | exon | 88585270 | 88585351 | 88585270 | ID=MsaT015576.1.exon2;Parent=MsaT015576.1 |
| Chr3 | CDS | 88585270 | 88585351 | 88585270 | ID=cds.MsaT015576.1;Parent=MsaT015576.1 |
| Chr3 | exon | 88585447 | 88585490 | 88585447 | ID=MsaT015576.1.exon1;Parent=MsaT015576.1 |
| Chr3 | CDS | 88585447 | 88585490 | 88585447 | ID=cds.MsaT015576.1;Parent=MsaT015576.1 |
| Gene Sequence |
| Protein sequence |
