AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015582

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015582 MtrunA17_Chr3g0120841 79.255 564 86 7 1 534 1 563 0.0 868
MsaG015582 MtrunA17_Chr3g0120201 97.736 265 6 0 271 535 1 265 0.0 527
MsaG015582 MtrunA17_Chr2g0290321 49.455 550 258 8 1 532 1 548 1.72e-172 498
MsaG015582 MtrunA17_Chr7g0239471 46.408 515 227 7 17 531 18 483 1.20e-144 425
MsaG015582 MtrunA17_Chr2g0290341 44.576 507 244 9 37 532 23 503 1.01e-136 405
MsaG015582 MtrunA17_Chr3g0120221 71.351 185 48 3 1 182 1 183 4.67e-89 271
MsaG015582 MtrunA17_Chr3g0120191 97.403 77 2 0 106 182 11 87 1.75e-49 166
MsaG015582 MtrunA17_Chr3g0120231 38.462 208 119 2 331 532 2 206 3.68e-41 147
MsaG015582 MtrunA17_Chr3g0120181 98.305 59 1 0 36 94 1 59 4.61e-39 136
MsaG015582 MtrunA17_Chr7g0238851 58.442 77 32 0 18 94 5 81 2.03e-24 97.8
MsaG015582 MtrunA17_Chr3g0124951 38.000 100 59 3 47 145 338 435 1.33e-11 67.4
MsaG015582 MtrunA17_Chr7g0233581 51.562 64 13 1 119 182 117 162 3.48e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015582 AT3G07740.1 50.719 556 238 11 1 532 1 544 1.26e-172 499
MsaG015582 AT3G07740.4 50.089 563 238 12 1 532 1 551 8.29e-170 492
MsaG015582 AT3G07740.3 51.544 518 219 9 36 532 17 523 2.33e-166 482
MsaG015582 AT4G16420.2 48.790 537 217 10 1 534 1 482 4.51e-166 480
MsaG015582 AT4G16420.1 48.704 540 217 10 1 534 1 486 3.29e-165 478
MsaG015582 AT4G16420.3 47.963 540 220 9 1 534 1 485 7.45e-164 474
MsaG015582 AT3G07740.2 51.553 483 205 8 68 532 2 473 2.92e-153 447

Find 98 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTAACTCCATCATTATTT+TGG 0.150007 3:-88662210 None:intergenic
TTTGGCAGAAATGGAATTTA+AGG 0.163949 3:+88663615 MsaT015582.1:CDS
CAAAAGAATAAAAGACTTAA+AGG 0.230732 3:+88664210 MsaT015582.1:CDS
TAGATATGCATCAGATTTCT+TGG 0.239365 3:-88665346 None:intergenic
GAAAAGAAGCCTAAATTATC+TGG 0.247074 3:+88663502 MsaT015582.1:CDS
GTAGGATTTGTCTTATTCTA+TGG 0.253298 3:+88660920 MsaT015582.1:intron
TATGATATGCTTGTGGAAAA+AGG 0.255246 3:+88665405 MsaT015582.1:CDS
GCCATTGACATGCATGGATT+TGG 0.256788 3:+88660711 MsaT015582.1:CDS
GTTCACATTGGAAGCCTTCT+TGG 0.280186 3:-88662188 None:intergenic
TGCATAGAGTGCTTCTTTGT+TGG 0.282569 3:+88659219 MsaT015582.1:CDS
TCACATATTTGAAGCTCTTC+AGG 0.296633 3:-88664103 None:intergenic
ATAAAAGCAATCATCTATAT+CGG 0.303117 3:+88659259 MsaT015582.1:CDS
ACATGCATGGATTTGGGAAT+TGG 0.309773 3:+88660718 MsaT015582.1:CDS
CAATGATGCTGAGCAAGTTT+TGG 0.319202 3:+88663597 MsaT015582.1:CDS
TTATATTCCCCAGATAATTT+AGG 0.358245 3:-88663511 None:intergenic
CCATTGACATGCATGGATTT+GGG 0.362070 3:+88660712 MsaT015582.1:CDS
CCAAAATAATGATGGAGTTA+AGG 0.371137 3:+88662210 MsaT015582.1:CDS
TGTCTTTGTGATAGCTTGTA+TGG 0.382689 3:-88665007 None:intergenic
AGAGTCAGCTTTGCATTTGA+AGG 0.391333 3:-88661877 None:intergenic
TTGCAGGAAGCTAGAATTGC+TGG 0.400705 3:+88664841 MsaT015582.1:intron
TATGGCTGGCGGTGCATCCT+TGG 0.406764 3:-88664989 None:intergenic
GGTGAGAGAAATTTCTCAAA+AGG 0.408187 3:-88664082 None:intergenic
ATCAATTTATTGAACAAAAG+AGG 0.414539 3:+88664887 MsaT015582.1:CDS
CAATCATCTATATCGGGTTA+TGG 0.418521 3:+88659266 MsaT015582.1:CDS
TATAAAGAAAGTGGTCAGAT+TGG 0.427378 3:+88664934 MsaT015582.1:CDS
GAAGCCTTCTTGGTTATACT+TGG 0.427656 3:-88662178 None:intergenic
CACAAAGACACTAGAAGAAT+GGG 0.442742 3:+88665020 MsaT015582.1:CDS
TAAATTCTGTCGATCTTGCT+TGG 0.443629 3:-88665384 None:intergenic
CCTCTCAACACTTCAATAGA+TGG 0.448042 3:-88663535 None:intergenic
GCTCATCTGCTCACGACAAC+AGG 0.449400 3:+88656591 MsaT015582.1:CDS
TAGGATTTGTCTTATTCTAT+GGG 0.455897 3:+88660921 MsaT015582.1:intron
TTCCGCTTACATCTTCAGAT+TGG 0.461749 3:+88660554 MsaT015582.1:CDS
TGAAGAGAAGTTACTTATTG+AGG 0.462048 3:+88660585 MsaT015582.1:CDS
TCACAAAGACACTAGAAGAA+TGG 0.467289 3:+88665019 MsaT015582.1:CDS
AGAAATTTCTCAAAAGGATC+GGG 0.467873 3:-88664076 None:intergenic
TCAGATATACAACATTATAA+TGG 0.471496 3:-88660791 None:intergenic
AATGATGGAGTTAAGGTTGA+AGG 0.474514 3:+88662217 MsaT015582.1:CDS
TCTCAAGCAGGTTGGAGCAC+TGG 0.475857 3:+88663478 MsaT015582.1:CDS
CCGCGTAATGTTGAGGAAGA+TGG 0.476953 3:-88665289 None:intergenic
AAAAGAAGCCTAAATTATCT+GGG 0.481337 3:+88663503 MsaT015582.1:CDS
ACATCTTCAGATTGGAGTGC+AGG 0.490115 3:+88660562 MsaT015582.1:CDS
CCCAAATCCATGCATGTCAA+TGG 0.493456 3:-88660712 None:intergenic
GGCGGTGCATCCTTGGTACT+AGG 0.496097 3:-88664982 None:intergenic
TTTACTTTATTCCATCAGAA+AGG 0.497295 3:-88661912 None:intergenic
AAAGAACCTCACCTTTCTGA+TGG 0.498619 3:+88661901 MsaT015582.1:CDS
TAAAAGCAATCATCTATATC+GGG 0.500162 3:+88659260 MsaT015582.1:CDS
GAGAAATTTCTCAAAAGGAT+CGG 0.500929 3:-88664077 None:intergenic
GATTTAGAAGCTTCATATGC+AGG 0.502552 3:+88659007 MsaT015582.1:CDS
TTTGTGATAGCTTGTATGGC+TGG 0.504362 3:-88665003 None:intergenic
AATGTGAACCAAAATAATGA+TGG 0.504833 3:+88662202 MsaT015582.1:CDS
GAACTCCTTGCCATGGCAAA+GGG 0.508221 3:+88660957 MsaT015582.1:CDS
CGCGTAATGTTGAGGAAGAT+GGG 0.509064 3:-88665288 None:intergenic
TCAAATAGCCTTACAGGTAG+AGG 0.513546 3:-88660828 None:intergenic
AGATGAGCGTGACGCGGCGC+GGG 0.514459 3:-88656576 None:intergenic
TTGTAGGCCATTGACATGCA+TGG 0.517859 3:+88660705 MsaT015582.1:intron
AAGATGTAAGCGGAAAAGAT+AGG 0.520508 3:-88660546 None:intergenic
TCAAATATGTGAACAGTACA+AGG 0.524557 3:+88664114 MsaT015582.1:CDS
CATGCAGTTAGAAATTTCAA+AGG 0.524634 3:+88665314 MsaT015582.1:CDS
CTATAGATTGTACCTGAGGT+TGG 0.533420 3:-88662055 None:intergenic
GGAACTCCTTGCCATGGCAA+AGG 0.542105 3:+88660956 MsaT015582.1:CDS
ATGCAGTTAGAAATTTCAAA+GGG 0.542710 3:+88665315 MsaT015582.1:CDS
CAGCTCTGAAGAATCTAAGA+AGG 0.544590 3:+88662003 MsaT015582.1:intron
CAAGGTAACTATAAAGAAAG+TGG 0.546753 3:+88664925 MsaT015582.1:CDS
CAGAATTTATGATATGCTTG+TGG 0.548783 3:+88665398 MsaT015582.1:CDS
TCTTCCAAGTATAACCAAGA+AGG 0.569053 3:+88662174 MsaT015582.1:CDS
GAGTTGATGTCCCTTTGCCA+TGG 0.571402 3:-88660967 None:intergenic
ATGGTGTTGCTGAAAACGTT+GGG 0.572743 3:+88660742 MsaT015582.1:CDS
AACATGTCAAGGCCAACCTC+AGG 0.574063 3:+88662043 MsaT015582.1:CDS
TTGTTAGTAGAATCTCAAGC+AGG 0.574478 3:+88663466 MsaT015582.1:intron
GATGTCCCTTTGCCATGGCA+AGG 0.577679 3:-88660962 None:intergenic
AAGGGACATCAACTCAAGAA+AGG 0.579536 3:+88660975 MsaT015582.1:CDS
AGTGGTCAGATTGGAACAAG+TGG 0.587270 3:+88664943 MsaT015582.1:CDS
CATCTATTGAAGTGTTGAGA+GGG 0.593154 3:+88663536 MsaT015582.1:CDS
GCATGATCCGCGTAATGTTG+AGG 0.597253 3:-88665296 None:intergenic
CATGGATTTGGGAATTGGAA+TGG 0.599035 3:+88660723 MsaT015582.1:CDS
TAGTAGAATCTCAAGCAGGT+TGG 0.601506 3:+88663470 MsaT015582.1:intron
CAGATGAGCGTGACGCGGCG+CGG 0.601734 3:-88656577 None:intergenic
CCATCTATTGAAGTGTTGAG+AGG 0.602727 3:+88663535 MsaT015582.1:CDS
CCACTAATCAAAACATGTCA+AGG 0.604753 3:+88662032 MsaT015582.1:CDS
CTTACAGGTAGAGGAAAACA+TGG 0.611599 3:-88660819 None:intergenic
CCATCTTCCTCAACATTACG+CGG 0.613654 3:+88665289 MsaT015582.1:CDS
TGAACGTGAAATGAAACTGC+AGG 0.617265 3:+88663654 MsaT015582.1:CDS
CAAAGAGGAACTCCTTGCCA+TGG 0.618565 3:+88660950 MsaT015582.1:CDS
AGAATCATTTCCTAGTACCA+AGG 0.625963 3:+88664972 MsaT015582.1:CDS
TTTATTCCATCAGAAAGGTG+AGG 0.628847 3:-88661907 None:intergenic
AGGACAAAAGAAGCTGAACA+AGG 0.637958 3:+88664907 MsaT015582.1:CDS
GTGAGCAGATGAGCGTGACG+CGG 0.650772 3:-88656582 None:intergenic
TCACTCAAAAGAAGAGCATG+AGG 0.651691 3:+88664150 MsaT015582.1:CDS
TTCTATGGGAAAGAACAAAG+AGG 0.651784 3:+88660935 MsaT015582.1:CDS
CTCCAATCTGAAGATGTAAG+CGG 0.651949 3:-88660556 None:intergenic
GTGATAGCTTGTATGGCTGG+CGG 0.658411 3:-88665000 None:intergenic
AATGGTGTTGCTGAAAACGT+TGG 0.659458 3:+88660741 MsaT015582.1:CDS
AACAGTACAAGGTCTTCATG+CGG 0.668099 3:+88664125 MsaT015582.1:CDS
GCAGGCATGGGAATAACTGA+TGG 0.676294 3:+88659105 MsaT015582.1:intron
AAAGAAGCCTAAATTATCTG+GGG 0.686550 3:+88663504 MsaT015582.1:CDS
AGAGGGTACACTTCTAACAG+AGG 0.710101 3:+88663553 MsaT015582.1:CDS
ATGAAACTGCAGGTGCTCCG+TGG 0.711759 3:+88663664 MsaT015582.1:CDS
GTAATGTTGAGGAAGATGGG+TGG 0.746783 3:-88665285 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 88656566 88665443 88656566 ID=MsaG015582
Chr3 mRNA 88656566 88665443 88656566 ID=MsaT015582.1;Parent=MsaG015582
Chr3 exon 88656566 88656612 88656566 ID=MsaT015582.1.exon1;Parent=MsaT015582.1
Chr3 CDS 88656566 88656612 88656566 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88658973 88659028 88658973 ID=MsaT015582.1.exon2;Parent=MsaT015582.1
Chr3 CDS 88658973 88659028 88658973 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88659109 88659287 88659109 ID=MsaT015582.1.exon3;Parent=MsaT015582.1
Chr3 CDS 88659109 88659287 88659109 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88660541 88660606 88660541 ID=MsaT015582.1.exon4;Parent=MsaT015582.1
Chr3 CDS 88660541 88660606 88660541 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88660711 88660836 88660711 ID=MsaT015582.1.exon5;Parent=MsaT015582.1
Chr3 CDS 88660711 88660836 88660711 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88660924 88660996 88660924 ID=MsaT015582.1.exon6;Parent=MsaT015582.1
Chr3 CDS 88660924 88660996 88660924 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88661872 88661929 88661872 ID=MsaT015582.1.exon7;Parent=MsaT015582.1
Chr3 CDS 88661872 88661929 88661872 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88662006 88662064 88662006 ID=MsaT015582.1.exon8;Parent=MsaT015582.1
Chr3 CDS 88662006 88662064 88662006 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88662161 88662238 88662161 ID=MsaT015582.1.exon9;Parent=MsaT015582.1
Chr3 CDS 88662161 88662238 88662161 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88663473 88663698 88663473 ID=MsaT015582.1.exon10;Parent=MsaT015582.1
Chr3 CDS 88663473 88663698 88663473 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88664021 88664231 88664021 ID=MsaT015582.1.exon11;Parent=MsaT015582.1
Chr3 CDS 88664021 88664231 88664021 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88664847 88665083 88664847 ID=MsaT015582.1.exon12;Parent=MsaT015582.1
Chr3 CDS 88664847 88665083 88664847 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Chr3 exon 88665252 88665443 88665252 ID=MsaT015582.1.exon13;Parent=MsaT015582.1
Chr3 CDS 88665252 88665443 88665252 ID=cds.MsaT015582.1;Parent=MsaT015582.1
Gene Sequence

>MsaT015582.1

ATGGGTCAGTCCCGCGCCGCGTCACGCTCATCTGCTCACGACAACAGGTCCAAGAGAAAAAAGGCTGCTTTAAATGCTGATGATTTAGAAGCTTCATATGCAGGCATGGGAATAACTGATGGCAAAGTATCACTATATCACTGTAATTACTGCAAAAAAAATATTTCAGGGAAGATTCACATCAAATGCGCTGTGTGTCAAGATTTTGATCTCTGCATAGAGTGCTTCTTTGTTGGTGCTGAGTTGACTCCTCATAAAAGCAATCATCTATATCGGGTTATGGATAGCCTATCTTTTCCGCTTACATCTTCAGATTGGAGTGCAGGTGAAGAGAAGTTACTTATTGAGGCCATTGACATGCATGGATTTGGGAATTGGAATGGTGTTGCTGAAAACGTTGGGACAAAAAGCAAATCTCAGTGTATTGACCATTATAATGTTGTATATCTGAATTCGCCATGTTTTCCTCTACCTGATTTGTCTTATTCTATGGGAAAGAACAAAGAGGAACTCCTTGCCATGGCAAAGGGACATCAACTCAAGAAAGATTTTCCTTCAAATGCAAAGCTGACTCTGAAAGAACCTCACCTTTCTGATGGAATAAACTCTGAAGAATCTAAGAAGGCAGAGACCACTAATCAAAACATGTCAAGGCCAACCTCAGCACGCGATGAAGCTCTTCCAAGTATAACCAAGAAGGCTTCCAATGTGAACCAAAATAATGATGGAGTTAAGGTTGAAGTAGAATCTCAAGCAGGTTGGAGCACTGGTGAAAAGAAGCCTAAATTATCTGGGGAATATAAGCCATCTATTGAAGTGTTGAGAGGGTACACTTCTAACAGAGGTGAATTTGAGATTGAATACGACAATGATGCTGAGCAAGTTTTGGCAGAAATGGAATTTAAGGACACTGATACTGAAGCTGAACGTGAAATGAAACTGCAGGTGCTCCGTGGCTACAGAAAAAAGTTAGATGAACGAAAGCGCAGAAAAGAATTTATACTTGAAAGAAATTTACTTTGTCCCGATCCTTTTGAGAAATTTCTCTCACCTGAAGAGCTTCAAATATGTGAACAGTACAAGGTCTTCATGCGGTTTCACTCAAAAGAAGAGCATGAGGAGTTGCTGCAAACTGCTATTCGAGAACACCGACTTGCCAAAAGAATAAAAGACTTAAAGGAAGCTAGAATTGCTGGCTGTGTAACTTCTGATGAAGCTTATCAATTTATTGAACAAAAGAGGACAAAAGAAGCTGAACAAGGTAACTATAAAGAAAGTGGTCAGATTGGAACAAGTGGCAAGACAGAATCATTTCCTAGTACCAAGGATGCACCGCCAGCCATACAAGCTATCACAAAGACACTAGAAGAATGGGATATTAGTGATTTTGAAGGGGCTGAATTGCTATCTGAATCTGAGATAAAGCTTTGTAATGAGATCAGAATGCTACCACCCATCTTCCTCAACATTACGCGGATCATGCAGTTAGAAATTTCAAAGGGCAGAGTAACCAAGAAATCTGATGCATATCTACTGTTCAAGTTCTCTCCAAGCAAGATCGACAGAATTTATGATATGCTTGTGGAAAAAGGAGTTGTTCAAGCATAA
Protein sequence

>MsaT015582.1

MGQSRAASRSSAHDNRSKRKKAALNADDLEASYAGMGITDGKVSLYHCNYCKKNISGKIHIKCAVCQDFDLCIECFFVGAELTPHKSNHLYRVMDSLSFPLTSSDWSAGEEKLLIEAIDMHGFGNWNGVAENVGTKSKSQCIDHYNVVYLNSPCFPLPDLSYSMGKNKEELLAMAKGHQLKKDFPSNAKLTLKEPHLSDGINSEESKKAETTNQNMSRPTSARDEALPSITKKASNVNQNNDGVKVEVESQAGWSTGEKKPKLSGEYKPSIEVLRGYTSNRGEFEIEYDNDAEQVLAEMEFKDTDTEAEREMKLQVLRGYRKKLDERKRRKEFILERNLLCPDPFEKFLSPEELQICEQYKVFMRFHSKEEHEELLQTAIREHRLAKRIKDLKEARIAGCVTSDEAYQFIEQKRTKEAEQGNYKESGQIGTSGKTESFPSTKDAPPAIQAITKTLEEWDISDFEGAELLSESEIKLCNEIRMLPPIFLNITRIMQLEISKGRVTKKSDAYLLFKFSPSKIDRIYDMLVEKGVVQA*