Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015754 | XP_024633575.1 | 71.815 | 259 | 58 | 4 | 21 | 267 | 1 | 256 | 1.74e-105 | 317 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015754 | tr|G7JBB9|G7JBB9_MEDTR | 75.097 | 257 | 23 | 5 | 21 | 264 | 1 | 229 | 1.39e-96 | 292 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG015754 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015754 | MtrunA17_Chr3g0122071 | 72.374 | 257 | 23 | 4 | 21 | 264 | 1 | 222 | 4.16e-95 | 279 |
| MsaG015754 | MtrunA17_Chr4g0064011 | 56.048 | 248 | 92 | 7 | 21 | 267 | 1 | 232 | 1.57e-70 | 217 |
| MsaG015754 | MtrunA17_Chr6g0474091 | 45.455 | 132 | 59 | 6 | 21 | 152 | 1 | 119 | 4.23e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015754 | AT2G18490.1 | 58.462 | 65 | 27 | 0 | 148 | 212 | 147 | 211 | 1.42e-19 | 85.9 |
| MsaG015754 | AT2G18490.2 | 58.462 | 65 | 27 | 0 | 148 | 212 | 197 | 261 | 1.99e-19 | 86.3 |
| MsaG015754 | AT2G15740.1 | 53.247 | 77 | 32 | 1 | 145 | 221 | 219 | 291 | 8.11e-19 | 84.7 |
| MsaG015754 | AT5G22990.1 | 50.602 | 83 | 40 | 1 | 156 | 237 | 232 | 314 | 3.33e-18 | 83.2 |
| MsaG015754 | AT5G42640.1 | 51.471 | 68 | 29 | 1 | 149 | 212 | 218 | 285 | 1.07e-16 | 78.6 |
Find 37 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGTCTTGAAGCACAATT+TGG | 0.219378 | 3:-90612868 | MsaT015754.1:CDS |
| TCAGTTGGTACCAATAATTT+TGG | 0.290051 | 3:-90612922 | MsaT015754.1:CDS |
| AGCATGACTACCAAAATTAT+TGG | 0.316188 | 3:+90612912 | None:intergenic |
| CAATCTATGATTGTTCATAT+TGG | 0.336228 | 3:+90612987 | None:intergenic |
| AGCCTACCACACAAGAAATA+TGG | 0.358104 | 3:-90612562 | MsaT015754.1:CDS |
| TATTAATAATAATTATTTGA+AGG | 0.406891 | 3:+90612253 | None:intergenic |
| ACAATCATAGATTGGTTCAA+TGG | 0.452424 | 3:-90612978 | MsaT015754.1:CDS |
| TTCTAGGATTCGAAAGAGAC+CGG | 0.465129 | 3:-90612425 | MsaT015754.1:CDS |
| AGCAATGAAAATATTGATGA+TGG | 0.472887 | 3:-90612595 | MsaT015754.1:CDS |
| ATTCAAGATGAGAAGGAAAA+GGG | 0.478078 | 3:+90613016 | None:intergenic |
| CAATATGAACAATCATAGAT+TGG | 0.479701 | 3:-90612986 | MsaT015754.1:CDS |
| TTATCAGCACAAGCAATAAG+AGG | 0.487551 | 3:+90612358 | None:intergenic |
| AGAGTCTTGAAGCACAATTT+GGG | 0.488266 | 3:-90612867 | MsaT015754.1:CDS |
| ATTTCTTGTGTGGTAGGCTG+TGG | 0.491007 | 3:+90612566 | None:intergenic |
| GATTCAAGATGAGAAGGAAA+AGG | 0.497647 | 3:+90613015 | None:intergenic |
| GATAAGCCTACATGGAGATC+AGG | 0.504896 | 3:+90612715 | None:intergenic |
| ATGATTGTTCATATTGGTGG+TGG | 0.517024 | 3:+90612993 | None:intergenic |
| TCACATCCTGATCTCCATGT+AGG | 0.522755 | 3:-90612721 | MsaT015754.1:CDS |
| TGAAGTGTGTTTAGAATCCA+TGG | 0.537305 | 3:+90613044 | None:intergenic |
| TTCAAGATGAGAAGGAAAAG+GGG | 0.537449 | 3:+90613017 | None:intergenic |
| TCTATGATTGTTCATATTGG+TGG | 0.538024 | 3:+90612990 | None:intergenic |
| ACCAACTGATGATGATGAAT+GGG | 0.541850 | 3:+90612936 | None:intergenic |
| GTCCATATTTCTTGTGTGGT+AGG | 0.551362 | 3:+90612560 | None:intergenic |
| ATGTTAACAAGAACGATATG+AGG | 0.552379 | 3:-90612633 | MsaT015754.1:CDS |
| CTCTCACCCCTCTTCCAAGT+GGG | 0.558684 | 3:-90612294 | MsaT015754.1:CDS |
| TACCAACTGATGATGATGAA+TGG | 0.562352 | 3:+90612935 | None:intergenic |
| GTGGTGGATTCAAGATGAGA+AGG | 0.572558 | 3:+90613009 | None:intergenic |
| AGATTGGTTCAATGGATGCA+TGG | 0.584866 | 3:-90612970 | MsaT015754.1:CDS |
| GCTCTCACCCCTCTTCCAAG+TGG | 0.584935 | 3:-90612295 | MsaT015754.1:CDS |
| TATGGTCCATATTTCTTGTG+TGG | 0.586966 | 3:+90612556 | None:intergenic |
| ACCCATTCATCATCATCAGT+TGG | 0.590463 | 3:-90612937 | MsaT015754.1:CDS |
| CAGATTCAAAAGCTCAATGA+CGG | 0.593887 | 3:-90612397 | MsaT015754.1:CDS |
| TCTAGGATTCGAAAGAGACC+GGG | 0.623769 | 3:-90612424 | MsaT015754.1:CDS |
| TCCGGGAATCATAAAAGCGA+TGG | 0.628214 | 3:-90612890 | MsaT015754.1:CDS |
| AAGTGGGATAAAAGTCAAGT+CGG | 0.648453 | 3:-90612278 | MsaT015754.1:CDS |
| GACGACGACGATGTTGATGT+AGG | 0.650410 | 3:+90612838 | None:intergenic |
| ATTGTGACGATAAGCCTACA+TGG | 0.663326 | 3:+90612707 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 90612255 | 90613064 | 90612255 | ID=MsaG015754 |
| Chr3 | mRNA | 90612255 | 90613064 | 90612255 | ID=MsaT015754.1;Parent=MsaG015754 |
| Chr3 | exon | 90612255 | 90613064 | 90612255 | ID=MsaT015754.1.exon1;Parent=MsaT015754.1 |
| Chr3 | CDS | 90612255 | 90613064 | 90612255 | ID=cds.MsaT015754.1;Parent=MsaT015754.1 |
| Gene Sequence |
| Protein sequence |
