Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015788 | PNY06680.1 | 86.806 | 144 | 12 | 2 | 1 | 138 | 5 | 147 | 1.18e-76 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015788 | tr|A0A2K3NUG6|A0A2K3NUG6_TRIPR | 86.806 | 144 | 12 | 2 | 1 | 138 | 5 | 147 | 5.65e-77 | 236 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG015788 | TF | NF-YC |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015788 | MtrunA17_Chr3g0122571 | 92.424 | 132 | 9 | 1 | 1 | 132 | 18 | 148 | 1.61e-80 | 235 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015788 | AT5G43250.1 | 57.143 | 133 | 51 | 3 | 3 | 134 | 2 | 129 | 4.93e-44 | 142 |
| MsaG015788 | AT3G12480.1 | 38.961 | 77 | 46 | 1 | 11 | 87 | 5 | 80 | 3.59e-11 | 60.1 |
| MsaG015788 | AT1G08970.3 | 34.400 | 125 | 72 | 4 | 3 | 118 | 68 | 191 | 3.86e-11 | 59.7 |
| MsaG015788 | AT1G08970.4 | 34.400 | 125 | 72 | 4 | 3 | 118 | 68 | 191 | 3.86e-11 | 59.7 |
| MsaG015788 | AT1G08970.2 | 34.400 | 125 | 72 | 4 | 3 | 118 | 68 | 191 | 3.86e-11 | 59.7 |
| MsaG015788 | AT1G08970.1 | 34.400 | 125 | 72 | 4 | 3 | 118 | 68 | 191 | 3.86e-11 | 59.7 |
Find 45 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCCATGGGTTCCGGTTC+AGG | 0.246040 | 3:+90989045 | None:intergenic |
| TCGAGTAGAAAATCACTTAT+TGG | 0.284969 | 3:+90989246 | None:intergenic |
| CAACTTCGGCTTCAGACTTT+CGG | 0.357492 | 3:+90989115 | None:intergenic |
| ATGGGTTCCGGTTCAGGTTC+AGG | 0.371834 | 3:+90989051 | None:intergenic |
| GAGTTCAGAAGCGTTGTTCT+TGG | 0.377847 | 3:-90989391 | MsaT015788.1:CDS |
| TCAGGTTCCGACTCAGGTTC+CGG | 0.385853 | 3:+90989069 | None:intergenic |
| TATGCCTCCTCCATGGGTTC+CGG | 0.409906 | 3:+90989039 | None:intergenic |
| ATCTTCTTCACTCTACTCTT+CGG | 0.421448 | 3:+90989444 | None:intergenic |
| CGATCCGCAACCGCCGCCGC+TGG | 0.421993 | 3:+90989183 | None:intergenic |
| GTACCAACCGGAACAGATTT+AGG | 0.424572 | 3:+90989159 | None:intergenic |
| GTCGGAACCTGAACCTGAAC+CGG | 0.433867 | 3:-90989058 | MsaT015788.1:CDS |
| GGATCGGCCTAAATCTGTTC+CGG | 0.447912 | 3:-90989166 | MsaT015788.1:CDS |
| GTTCTTGGTGTCACGATCTA+CGG | 0.459375 | 3:-90989376 | MsaT015788.1:CDS |
| TCCGACTCAGGTTCCGGCGG+TGG | 0.464476 | 3:+90989075 | None:intergenic |
| CAGCGGCGGCGGTTGCGGAT+CGG | 0.466428 | 3:-90989182 | MsaT015788.1:CDS |
| TAAACCAGCGGCGGCGGTTG+CGG | 0.474107 | 3:-90989187 | MsaT015788.1:CDS |
| ATCTGATAAACCAGCGGCGG+CGG | 0.487271 | 3:-90989193 | MsaT015788.1:CDS |
| TCAGGTTCAGGTTCCGACTC+AGG | 0.489777 | 3:+90989063 | None:intergenic |
| CACAACCTATGCCTCCTCCA+TGG | 0.514005 | 3:+90989032 | None:intergenic |
| ACTCTACTCTTCGGAAACTC+TGG | 0.522689 | 3:+90989453 | None:intergenic |
| TCAATGCGGCGAGTACCAAC+CGG | 0.541474 | 3:+90989147 | None:intergenic |
| ACAACCTATGCCTCCTCCAT+GGG | 0.542610 | 3:+90989033 | None:intergenic |
| CTCTGGACAAAGACGTGAAG+AGG | 0.546039 | 3:-90989416 | MsaT015788.1:CDS |
| TTCGCAGTTTCCGACGGCAT+CGG | 0.549112 | 3:+90989216 | None:intergenic |
| AATTGCAATTGTTATGGCAG+AGG | 0.550560 | 3:-90989502 | None:intergenic |
| TTGCGCTTGAGCATCAACTT+CGG | 0.551263 | 3:+90989101 | None:intergenic |
| GGAACCCATGGAGGAGGCAT+AGG | 0.556067 | 3:-90989037 | None:intergenic |
| TCAGATTTCGCAGTTTCCGA+CGG | 0.560923 | 3:+90989210 | None:intergenic |
| GTAGAAAATCACTTATTGGA+CGG | 0.569952 | 3:+90989250 | None:intergenic |
| TGAACCTGAACCGGAACCCA+TGG | 0.579146 | 3:-90989049 | MsaT015788.1:CDS |
| TCAAGCGCAAGCGCCACCGC+CGG | 0.588095 | 3:-90989088 | MsaT015788.1:CDS |
| CGGCCTAAATCTGTTCCGGT+TGG | 0.590306 | 3:-90989162 | MsaT015788.1:CDS |
| AGTGAAGAAGATAATCACTC+TGG | 0.599750 | 3:-90989433 | MsaT015788.1:CDS |
| TGAACCGGAACCCATGGAGG+AGG | 0.608400 | 3:-90989043 | MsaT015788.1:CDS |
| GCCACCGCCGGAACCTGAGT+CGG | 0.613247 | 3:-90989076 | MsaT015788.1:CDS |
| TCTGGACAAAGACGTGAAGA+GGG | 0.629567 | 3:-90989415 | MsaT015788.1:CDS |
| CGATTCGCTTCCGATGCCGT+CGG | 0.664511 | 3:-90989226 | MsaT015788.1:CDS |
| ACCTGAACCGGAACCCATGG+AGG | 0.681479 | 3:-90989046 | MsaT015788.1:CDS |
| GGTTCCGACTCAGGTTCCGG+CGG | 0.689237 | 3:+90989072 | None:intergenic |
| TATTGAGAAGAAGCGCAAGA+CGG | 0.692763 | 3:-90989310 | MsaT015788.1:CDS |
| GTTACTTGCTGAAAAGTCTG+CGG | 0.696867 | 3:-90989340 | MsaT015788.1:CDS |
| GCTTGAACATATAAGAATCG+CGG | 0.698334 | 3:-90989283 | MsaT015788.1:CDS |
| GAAATCTGATAAACCAGCGG+CGG | 0.702085 | 3:-90989196 | MsaT015788.1:CDS |
| TGCGAAATCTGATAAACCAG+CGG | 0.703898 | 3:-90989199 | MsaT015788.1:CDS |
| TTCGGAAGATCTGATCAATG+CGG | 0.759168 | 3:+90989133 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 90989038 | 90989511 | 90989038 | ID=MsaG015788 |
| Chr3 | mRNA | 90989038 | 90989511 | 90989038 | ID=MsaT015788.1;Parent=MsaG015788 |
| Chr3 | exon | 90989038 | 90989511 | 90989038 | ID=MsaT015788.1.exon1;Parent=MsaT015788.1 |
| Chr3 | CDS | 90989038 | 90989511 | 90989038 | ID=cds.MsaT015788.1;Parent=MsaT015788.1 |
| Gene Sequence |
| Protein sequence |
