AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015805

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015805 MtrunA17_Chr3g0122391 94.456 992 29 2 1 966 36 1027 0.0 1927
MsaG015805 MtrunA17_Chr8g0377481 77.835 979 182 6 14 966 1 970 0.0 1529
MsaG015805 MtrunA17_Chr5g0447641 53.894 963 389 13 47 963 259 1212 0.0 1000
MsaG015805 MtrunA17_Chr8g0392301 50.501 998 398 17 38 965 25 996 0.0 924
MsaG015805 MtrunA17_Chr2g0311191 39.809 314 89 3 208 521 21 234 4.59e-60 205
MsaG015805 MtrunA17_Chr1g0150511 33.123 317 163 7 408 676 379 694 3.58e-34 142
MsaG015805 MtrunA17_Chr1g0150511 32.867 143 76 3 840 963 870 1011 4.76e-13 73.6
MsaG015805 MtrunA17_Chr8g0371601 31.329 316 190 5 361 663 427 728 5.37e-33 138
MsaG015805 MtrunA17_Chr8g0371601 35.172 145 69 4 824 951 1018 1154 1.81e-15 81.6
MsaG015805 MtrunA17_Chr5g0405701 28.608 388 233 11 309 670 404 773 6.47e-33 138
MsaG015805 MtrunA17_Chr5g0405701 34.932 146 64 2 832 951 1044 1184 1.41e-16 85.1
MsaG015805 MtrunA17_Chr4g0013831 28.530 347 218 6 396 738 335 655 1.69e-32 135
MsaG015805 MtrunA17_Chr4g0013831 33.613 119 71 3 837 955 614 724 3.08e-11 67.4
MsaG015805 MtrunA17_Chr3g0136601 40.972 144 84 1 408 551 373 515 4.56e-25 112
MsaG015805 MtrunA17_Chr3g0136601 30.822 146 80 3 839 964 805 949 8.34e-14 75.9
MsaG015805 MtrunA17_Chr7g0269631 27.778 288 173 7 396 677 335 593 2.49e-22 102
MsaG015805 MtrunA17_Chr1g0194741 30.909 165 94 4 397 544 387 548 3.04e-17 86.7
MsaG015805 MtrunA17_Chr1g0197591 37.313 134 64 4 835 959 923 1045 1.27e-16 85.1
MsaG015805 MtrunA17_Chr1g0197591 26.568 271 179 7 398 654 371 635 4.92e-16 83.2
MsaG015805 MtrunA17_Chr7g0274831 36.220 127 67 4 837 959 638 754 1.58e-15 81.3
MsaG015805 MtrunA17_Chr7g0274831 22.222 369 244 9 396 757 366 698 4.66e-14 76.6
MsaG015805 MtrunA17_Chr8g0343551 46.914 81 32 3 879 959 13 82 4.22e-15 71.6
MsaG015805 MtrunA17_Chr1g0194731 30.380 158 87 6 814 960 304 449 2.53e-14 76.6
MsaG015805 MtrunA17_Chr1g0186431 31.298 131 82 3 834 963 631 754 4.08e-14 77.0
MsaG015805 MtrunA17_Chr1g0186431 25.503 149 111 0 384 532 344 492 8.43e-12 69.3
MsaG015805 MtrunA17_Chr1g0190021 35.200 125 72 3 837 959 634 751 6.78e-13 72.8
MsaG015805 MtrunA17_Chr1g0190021 22.751 378 256 11 384 757 350 695 1.30e-12 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015805 AT1G27320.1 65.691 991 280 12 14 965 61 1030 0.0 1268
MsaG015805 AT5G35750.1 54.348 966 372 15 47 964 230 1174 0.0 1004
MsaG015805 AT2G01830.1 51.643 974 393 15 44 964 101 1049 0.0 946
MsaG015805 AT2G01830.4 51.643 974 393 15 44 964 101 1049 0.0 946
MsaG015805 AT2G01830.6 51.643 974 393 15 44 964 101 1049 0.0 946
MsaG015805 AT2G01830.3 51.643 974 393 15 44 964 101 1049 0.0 946
MsaG015805 AT2G01830.5 51.643 974 393 15 44 964 113 1061 0.0 946
MsaG015805 AT2G01830.2 51.643 974 393 15 44 964 124 1072 0.0 944
MsaG015805 AT2G17820.1 30.675 326 193 8 361 670 454 762 6.94e-35 144
MsaG015805 AT2G17820.1 32.215 149 64 2 835 951 1041 1184 3.00e-14 78.2
MsaG015805 AT5G10720.1 33.835 266 156 4 408 672 369 615 4.21e-33 138
MsaG015805 AT5G10720.1 31.944 144 78 3 839 963 779 921 8.55e-15 79.7
MsaG015805 AT1G66340.1 29.107 347 212 8 396 738 334 650 8.07e-33 137
MsaG015805 AT2G40940.1 29.528 254 156 4 396 649 334 564 9.86e-24 107
MsaG015805 AT2G47430.1 26.557 305 180 8 393 670 383 670 3.08e-21 100
MsaG015805 AT2G47430.1 36.364 143 69 6 833 959 981 1117 1.38e-14 79.3
MsaG015805 AT3G04580.2 34.074 135 79 3 834 966 638 764 7.26e-15 79.7
MsaG015805 AT3G04580.2 22.222 369 255 8 396 757 358 701 6.84e-14 76.6
MsaG015805 AT3G04580.1 34.074 135 79 3 834 966 638 764 7.26e-15 79.7
MsaG015805 AT3G04580.1 22.222 369 255 8 396 757 358 701 6.84e-14 76.6
MsaG015805 AT3G23150.1 31.111 135 78 2 834 963 642 766 1.73e-11 68.9

Find 223 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCAAGCATCTTCTCTT+TGG 0.198734 3:+91205123 MsaT015805.1:CDS
ATATCTATTGATGTGCTTTC+TGG 0.199915 3:+91201946 MsaT015805.1:CDS
ATTGTTGCTGGCATGGGTTT+TGG 0.201381 3:+91201063 MsaT015805.1:CDS
GTTTGTTCATCTACCCATTT+TGG 0.201868 3:+91205766 MsaT015805.1:CDS
TTAACTTTGGAGATCCTTTC+AGG 0.201914 3:+91202802 MsaT015805.1:CDS
TTGCTGATGGTCGCCGCAGC+TGG 0.223040 3:+91204284 MsaT015805.1:CDS
GTTCGATATACGAGCAATTA+TGG 0.223643 3:+91203774 MsaT015805.1:CDS
CTCTCAAGCATCTTCTCTTT+GGG 0.233041 3:+91205124 MsaT015805.1:CDS
GTCAATTATAAATGAAGTTT+TGG 0.241047 3:+91203705 MsaT015805.1:CDS
GTTGCTTGAATCCTTTCATT+TGG 0.253731 3:-91202191 None:intergenic
TTGCTTGAATCCTTTCATTT+GGG 0.258270 3:-91202190 None:intergenic
GTCTGCGGTCATAGCCAAAA+TGG 0.274932 3:-91205780 None:intergenic
GTTGGTTTATAGTGTCTCTA+TGG 0.276176 3:+91201086 MsaT015805.1:CDS
GTTTGAAGCTCGTTTGAATT+AGG 0.284510 3:-91204616 None:intergenic
TTAACGAGAATAAATAGTTT+TGG 0.294927 3:-91204940 None:intergenic
TCAATTTGATGCATGTTTCA+TGG 0.295254 3:+91205278 MsaT015805.1:CDS
CCTGCAACCTCAGAGTTTAA+TGG 0.298082 3:+91204664 MsaT015805.1:CDS
CTTGTTATTGCACTCCTTGT+TGG 0.299522 3:+91203249 MsaT015805.1:CDS
AATGCATACCCATCCATTTC+AGG 0.300024 3:-91205312 None:intergenic
TCTCATGGGTAACTCGATTA+AGG 0.309745 3:+91204038 MsaT015805.1:CDS
CCCAAACCTCTTGTTCAATC+AGG 0.316912 3:-91204837 None:intergenic
ATGTGAGGATAACTCCTAAA+TGG 0.333003 3:-91202130 None:intergenic
TACACTGATAATACTGTTGA+TGG 0.335179 3:-91201003 None:intergenic
TCAGAACCATACATGGGAAT+TGG 0.339185 3:-91202744 None:intergenic
CGCACTTTGTGAATAACATT+AGG 0.352181 3:+91204881 MsaT015805.1:CDS
CTGCGGCGACCATCAGCAAC+TGG 0.353028 3:-91204280 None:intergenic
CCATTAAACTCTGAGGTTGC+AGG 0.355278 3:-91204664 None:intergenic
TCCCACACTTAGAGCATTCC+TGG 0.359931 3:-91205820 None:intergenic
CATGTGAGCTTTCTTAACTT+TGG 0.362535 3:+91202789 MsaT015805.1:CDS
CTGTTGAAAACAAACCATTT+AGG 0.364791 3:+91202116 MsaT015805.1:CDS
CTCTCTCAACTTCTATTGAA+TGG 0.365237 3:-91204216 None:intergenic
AAAGTTGTTGAAGTTGATAA+AGG 0.366112 3:+91204904 MsaT015805.1:CDS
CGGTTCTGACATATTCTTGT+TGG 0.374228 3:-91203657 None:intergenic
TCCTGATTGAACAAGAGGTT+TGG 0.378231 3:+91204836 MsaT015805.1:CDS
GGATATTCAAATGCCTGAAA+TGG 0.382771 3:+91205299 MsaT015805.1:CDS
TGATTATGTACCAGATGAAT+TGG 0.385215 3:+91201858 MsaT015805.1:CDS
ATTATTTGTCGAAACCTTCC+AGG 0.393734 3:-91204009 None:intergenic
CAGGTTCCTCAACAGCTTAT+AGG 0.395280 3:+91203982 MsaT015805.1:CDS
GATGCAGAACATACATTAAG+TGG 0.399490 3:+91204253 MsaT015805.1:CDS
GACCAGGCAAAGATTGAATC+TGG 0.399819 3:+91203727 MsaT015805.1:CDS
TGACAGGAGCATATTCTTCA+TGG 0.406008 3:-91201894 None:intergenic
TCATTTGGGGTAGCATTTGA+TGG 0.407780 3:-91202176 None:intergenic
CAACAGTATTATCAGTGTAT+TGG 0.408995 3:+91201007 MsaT015805.1:CDS
TGAAACATGCATCAAATTGA+TGG 0.409814 3:-91205275 None:intergenic
GTTAAAGTGCTTCCAATCTT+CGG 0.411695 3:-91204565 None:intergenic
ACATAATCATCCTCATGAAC+CGG 0.414182 3:-91201844 None:intergenic
TAACCTAAAACACCGTTCAT+TGG 0.415965 3:-91203506 None:intergenic
CCTGATTGAACAAGAGGTTT+GGG 0.417274 3:+91204837 MsaT015805.1:CDS
ATGTTGCGAGCAAGAGAATC+AGG 0.425892 3:+91202065 MsaT015805.1:CDS
TTTGTGGGCGAACCGAAGAT+TGG 0.428927 3:+91204553 MsaT015805.1:CDS
CTCGACACTTCAGCACGAAC+GGG 0.429876 3:-91204715 None:intergenic
GTATTGGTTCTAAAAGAGTT+AGG 0.431830 3:+91201023 MsaT015805.1:CDS
TGCTGATGGTCGCCGCAGCT+GGG 0.438301 3:+91204285 MsaT015805.1:CDS
TCTGAGAAATCTCAAGGAAA+AGG 0.441122 3:+91203817 MsaT015805.1:CDS
AATCAAACTGATGAGATCAT+TGG 0.444384 3:-91204365 None:intergenic
AGATGCATCCCCATCGGTTA+CGG 0.446690 3:-91204803 None:intergenic
AGCAGTTTATGTCTCGGATC+AGG 0.446805 3:+91203963 MsaT015805.1:CDS
CCCGTCGCCAACCTTGGAAA+TGG 0.448066 3:-91203210 None:intergenic
TAAATGTTGGGAGAAGATCT+TGG 0.452217 3:+91200964 MsaT015805.1:CDS
TCGAGAAAACACGGAGGAAC+TGG 0.453005 3:+91204475 MsaT015805.1:CDS
CAGAACCATACATGGGAATT+GGG 0.453538 3:-91202743 None:intergenic
TTGGCGACGGGTACATCAAT+TGG 0.453701 3:+91203222 MsaT015805.1:CDS
TAAAGTTGTTCAGCTTCAAA+TGG 0.453871 3:-91205863 None:intergenic
AAATGTTGGGAGAAGATCTT+GGG 0.457357 3:+91200965 MsaT015805.1:CDS
TTCAGTCACGAGGGACCTCT+TGG 0.458368 3:+91204322 MsaT015805.1:CDS
TTGGTAATCATCCTCTACTT+TGG 0.458958 3:-91203304 None:intergenic
TGGGGATGCATCTATCAATA+TGG 0.459409 3:+91204813 MsaT015805.1:CDS
TACCAGATTCAATCTTTGCC+TGG 0.460164 3:-91203729 None:intergenic
ACAAATAATTACAAATCTCA+TGG 0.460319 3:+91204023 MsaT015805.1:CDS
ATGTATGGTTCTGATGTATC+AGG 0.460892 3:+91202753 MsaT015805.1:CDS
GGTTCTGACATATTCTTGTT+GGG 0.465067 3:-91203656 None:intergenic
TGCTATGCAGGTATCTTGGT+GGG 0.467017 3:+91202622 MsaT015805.1:intron
TAACTGCTATGCAGGTATCT+TGG 0.469122 3:+91202618 MsaT015805.1:intron
TGTTTAGTGGGCCTCATGAA+TGG 0.472002 3:+91204520 MsaT015805.1:CDS
TCATGTCTTCATAATCCAAC+AGG 0.473191 3:+91204997 MsaT015805.1:CDS
GGGGATGGTTCCAATTCATC+TGG 0.475795 3:-91201868 None:intergenic
AACTCTGAGGTTGCAGGATG+AGG 0.480482 3:-91204658 None:intergenic
GGAGGAAATTGTTGCTGGCA+TGG 0.482367 3:+91201056 MsaT015805.1:CDS
CATTAGTATGGACACAAAGA+TGG 0.482671 3:+91200910 None:intergenic
AATGGAGAAATCAGATTTGT+GGG 0.482820 3:+91204538 MsaT015805.1:CDS
AAGCATTAACAAGTGTTTAG+TGG 0.483264 3:+91204507 MsaT015805.1:CDS
ATTGTGGAGGAAATTGTTGC+TGG 0.485555 3:+91201051 MsaT015805.1:CDS
GTCAGAACCGCACAAGGCAG+TGG 0.486290 3:+91203670 MsaT015805.1:CDS
GACTATCCATCTTGTTGAGG+AGG 0.486988 3:+91204189 MsaT015805.1:CDS
CAACAGCTTATAGGTGATCC+TGG 0.488540 3:+91203991 MsaT015805.1:CDS
GATGGACCTACCTGCATGAA+TGG 0.490106 3:-91204448 None:intergenic
GAGCATATTCTTCATGGATA+GGG 0.491204 3:-91201888 None:intergenic
AACAAGGGTGGAGTATTAAG+AGG 0.492013 3:+91201800 MsaT015805.1:CDS
TATTGATGTGCTTTCTGGAA+AGG 0.494599 3:+91201951 MsaT015805.1:CDS
GTTTGAAATAGAGTCATTAG+TGG 0.495045 3:+91202647 MsaT015805.1:CDS
TGTGACTATCCATCTTGTTG+AGG 0.496121 3:+91204186 MsaT015805.1:CDS
GATGGTCGCCGCAGCTGGGA+AGG 0.497475 3:+91204289 MsaT015805.1:CDS
TGCTCTAAGTGTGGGATGGA+TGG 0.499675 3:+91205827 MsaT015805.1:CDS
GATGTACCCGTCGCCAACCT+TGG 0.499801 3:-91203216 None:intergenic
GAAAGATGTGGCTTAAATGT+TGG 0.505804 3:+91200951 MsaT015805.1:CDS
GAAACCACCATTTCCAAGGT+TGG 0.506338 3:+91203203 MsaT015805.1:CDS
CTGCTATGCAGGTATCTTGG+TGG 0.507522 3:+91202621 MsaT015805.1:intron
ACACACCCAATTCCCATGTA+TGG 0.507762 3:+91202738 MsaT015805.1:CDS
GAGGAAATTGTTGCTGGCAT+GGG 0.509124 3:+91201057 MsaT015805.1:CDS
AAACACGGAGGAACTGGTAT+TGG 0.510837 3:+91204481 MsaT015805.1:CDS
GGAGCATATTCTTCATGGAT+AGG 0.512983 3:-91201889 None:intergenic
GTGGTGTTGCATATGCTGTA+AGG 0.513848 3:+91201743 MsaT015805.1:CDS
AGCTGATGTTGCTAAATCCC+AGG 0.514509 3:+91203359 MsaT015805.1:CDS
ACCATTTCCAAGGTTGGCGA+CGG 0.515570 3:+91203209 MsaT015805.1:CDS
TGATACATCAGAACCATACA+TGG 0.519775 3:-91202751 None:intergenic
ATTCAAATGCCTGAAATGGA+TGG 0.520727 3:+91205303 MsaT015805.1:CDS
CGTGATGCAGGCTACACACC+AGG 0.521945 3:+91205802 MsaT015805.1:CDS
ACAAGGCAGTGGAAAAGCTC+TGG 0.522119 3:+91203681 MsaT015805.1:CDS
AACAGAAACCACCATTTCCA+AGG 0.522429 3:+91203199 MsaT015805.1:CDS
GAATGGAGAAATCAGATTTG+TGG 0.523897 3:+91204537 MsaT015805.1:CDS
ACTTCAGCACGAACGGGTCT+CGG 0.525496 3:-91204709 None:intergenic
GAGCAAGAGAATCAGGCAAA+GGG 0.525841 3:+91202072 MsaT015805.1:CDS
GTGAATCGCACGGTAGCAGC+TGG 0.527329 3:+91205177 MsaT015805.1:CDS
TCTGCGGTCATAGCCAAAAT+GGG 0.530084 3:-91205779 None:intergenic
GGAAGCATGAGATGCACTGC+AGG 0.532148 3:+91202823 MsaT015805.1:CDS
AAGATTAGGTACCTGGTCAA+TGG 0.533997 3:-91201278 None:intergenic
TATGGTCCTGATTGAACAAG+AGG 0.534402 3:+91204831 MsaT015805.1:CDS
AAAGATGTGGCTTAAATGTT+GGG 0.536867 3:+91200952 MsaT015805.1:CDS
TGCTTGAATCCTTTCATTTG+GGG 0.537514 3:-91202189 None:intergenic
AGCATTAACAAGTGTTTAGT+GGG 0.537951 3:+91204508 MsaT015805.1:CDS
TCTCATCAGTTTGATTGTAT+CGG 0.538336 3:+91204372 MsaT015805.1:CDS
TATGACCGCAGACGTGATGC+AGG 0.539120 3:+91205790 MsaT015805.1:CDS
GGAAAACCTCCTCAACAAGA+TGG 0.540597 3:-91204195 None:intergenic
ACTTACACAACACTGAAAAG+AGG 0.541604 3:+91201125 MsaT015805.1:CDS
CAGAAGGATTCTTAGCATGA+TGG 0.541718 3:-91201255 None:intergenic
TAGGAGATGGTGGAAAGATG+TGG 0.542063 3:+91200939 MsaT015805.1:CDS
AAAGAGTTAGGAGAAAATTG+TGG 0.543247 3:+91201035 MsaT015805.1:CDS
AAAGTTAGCAGTTTATGTCT+CGG 0.543839 3:+91203957 MsaT015805.1:intron
AGTGGTTTCCCAGTTGCTGA+TGG 0.544028 3:+91204271 MsaT015805.1:CDS
GAAAGGATTCAAGCAACTGA+TGG 0.545412 3:+91202197 MsaT015805.1:CDS
TAAGGGTGCTTCAATCTGAA+AGG 0.545666 3:+91201761 MsaT015805.1:CDS
GGATGATTACCAAGAGATGA+TGG 0.546326 3:+91203314 MsaT015805.1:CDS
AAGGGTGCTTCAATCTGAAA+GGG 0.547300 3:+91201762 MsaT015805.1:CDS
AGGGATGATGAATATGCTCA+TGG 0.547816 3:+91203615 MsaT015805.1:intron
AGGGTGGAGTATTAAGAGGA+TGG 0.548363 3:+91201804 MsaT015805.1:CDS
GTATCTTGTGCAAAGATGAC+AGG 0.548377 3:-91201910 None:intergenic
TTTACAGCTGTGTTCACCAA+TGG 0.548416 3:+91204589 MsaT015805.1:CDS
TATGCAACACCACTTGTAAG+TGG 0.549161 3:-91201733 None:intergenic
ATGAACCGGGTTCTGCTCCA+TGG 0.549463 3:-91201830 None:intergenic
ATTAGTATGGACACAAAGAT+GGG 0.549576 3:+91200911 None:intergenic
GAAACATGCATCAAATTGAT+GGG 0.550648 3:-91205274 None:intergenic
AGCTTATAGGTGATCCTGGA+AGG 0.551023 3:+91203995 MsaT015805.1:CDS
GTTTGTCCACCGTAACCGAT+GGG 0.553657 3:+91204794 MsaT015805.1:CDS
GTTTGAATTAGGACATCCAT+TGG 0.553942 3:-91204605 None:intergenic
GATGAGATCATTGGAGGAGG+TGG 0.554361 3:-91204356 None:intergenic
CCATTTCCAAGGTTGGCGAC+GGG 0.554660 3:+91203210 MsaT015805.1:CDS
TGCATCTCATGCTTCCTGAA+AGG 0.558589 3:-91202816 None:intergenic
GGGTGGCCTCAGCATAGTGA+TGG 0.560543 3:-91205253 None:intergenic
TTCAAATGCCTGAAATGGAT+GGG 0.561638 3:+91205304 MsaT015805.1:CDS
AGTTTGTCCACCGTAACCGA+TGG 0.562427 3:+91204793 MsaT015805.1:CDS
ATCATACACATTAACAAGCA+CGG 0.567202 3:-91202703 None:intergenic
AGATATCACATACAAAGGCT+TGG 0.567391 3:+91204736 MsaT015805.1:CDS
GAATCCTTCTGCCATTGACC+AGG 0.567998 3:+91201267 MsaT015805.1:CDS
CAAATAATTACAAATCTCAT+GGG 0.572811 3:+91204024 MsaT015805.1:CDS
TATTCTTCATGGATAGGGGA+TGG 0.574785 3:-91201883 None:intergenic
TCTCGACACTTCAGCACGAA+CGG 0.575293 3:-91204716 None:intergenic
GCAAGCATACTTGCTCTCAA+AGG 0.577203 3:-91205030 None:intergenic
AAAGGATTCAAGCAACTGAT+GGG 0.577305 3:+91202198 MsaT015805.1:CDS
GGAGCAGAACCCGGTTCATG+AGG 0.577743 3:+91201834 MsaT015805.1:CDS
GATATTCACTCCATTCATGC+AGG 0.578156 3:+91204438 MsaT015805.1:CDS
AAGAGGTCCCTCGTGACTGA+AGG 0.581373 3:-91204320 None:intergenic
AGCTGAACAACTTTATAGAG+AGG 0.581697 3:+91205871 MsaT015805.1:CDS
CAAATGCTACCCCAAATGAA+AGG 0.586250 3:+91202180 MsaT015805.1:CDS
GGATCACCTATAAGCTGTTG+AGG 0.588527 3:-91203988 None:intergenic
AACAATTTGAGAAGCAACAA+GGG 0.589239 3:+91201785 MsaT015805.1:CDS
AGCATATTCTTCATGGATAG+GGG 0.589325 3:-91201887 None:intergenic
CACCAGGAATGCTCTAAGTG+TGG 0.589718 3:+91205818 MsaT015805.1:CDS
CAAACTGATGAGATCATTGG+AGG 0.589840 3:-91204362 None:intergenic
CGAGCAAGAGAATCAGGCAA+AGG 0.590048 3:+91202071 MsaT015805.1:CDS
CATGCATCAAATTGATGGGG+TGG 0.590211 3:-91205270 None:intergenic
CCATCCATCTCGAGAAAACA+CGG 0.590517 3:+91204466 MsaT015805.1:CDS
CAACAAGGAGTGCAATAACA+AGG 0.591437 3:-91203248 None:intergenic
TGCGGCGACCATCAGCAACT+GGG 0.592339 3:-91204279 None:intergenic
GTTTGGCAGTTCACAGACAA+AGG 0.593004 3:+91204154 MsaT015805.1:intron
TTTGAGAGCAAGTATGCTTG+CGG 0.593125 3:+91205032 MsaT015805.1:CDS
CTCTGAATCCTTCCCAGCTG+CGG 0.594301 3:-91204297 None:intergenic
GGATACCATGGAGCAGAACC+CGG 0.595639 3:+91201825 MsaT015805.1:CDS
CTGAGGTTGCAGGATGAGGT+AGG 0.598010 3:-91204654 None:intergenic
GTCGCCAACCTTGGAAATGG+TGG 0.598690 3:-91203207 None:intergenic
CATAATCATCCTCATGAACC+GGG 0.599931 3:-91201843 None:intergenic
ATCTGATTTCTCCATTCATG+AGG 0.602555 3:-91204531 None:intergenic
GAACAATTTGAGAAGCAACA+AGG 0.603982 3:+91201784 MsaT015805.1:CDS
TGTAGCCTGCATCACGTCTG+CGG 0.606500 3:-91205795 None:intergenic
GCTATGCAGGTATCTTGGTG+GGG 0.608136 3:+91202623 MsaT015805.1:intron
ATGAATGGAGTGAATATCAT+CGG 0.611011 3:-91204433 None:intergenic
TATTAAGAGGATGGATACCA+TGG 0.611537 3:+91201813 MsaT015805.1:CDS
TGGTGTTGCATATGCTGTAA+GGG 0.613148 3:+91201744 MsaT015805.1:CDS
ATCTCAAGGAAAAGGAGTGG+AGG 0.616379 3:+91203825 MsaT015805.1:CDS
TGCATCCCCATCGGTTACGG+TGG 0.616687 3:-91204800 None:intergenic
ACCAGGAATGCTCTAAGTGT+GGG 0.617004 3:+91205819 MsaT015805.1:CDS
TTTGAGAGGCCACTTACAAG+TGG 0.618617 3:+91201724 MsaT015805.1:CDS
ACTGATGAGATCATTGGAGG+AGG 0.619983 3:-91204359 None:intergenic
GAGTCGAATTGCCAAAGTAG+AGG 0.627075 3:+91203293 MsaT015805.1:CDS
TGTCGAGATATCACATACAA+AGG 0.627297 3:+91204731 MsaT015805.1:CDS
GGCTTCACTACAAAGAGCAG+TGG 0.630799 3:+91205053 MsaT015805.1:CDS
ATTGATAGATGCATCCCCAT+CGG 0.631985 3:-91204809 None:intergenic
GCATGTGTGATGAAAGAGCT+AGG 0.632094 3:+91201164 MsaT015805.1:CDS
GAATATGTCAGAACCGCACA+AGG 0.636751 3:+91203664 MsaT015805.1:CDS
GGTACCTGGTCAATGGCAGA+AGG 0.637480 3:-91201271 None:intergenic
TGATAATAGTGTGAATCGCA+CGG 0.638695 3:+91205167 MsaT015805.1:CDS
CATGACAAATATGTCCAACA+AGG 0.639906 3:-91203263 None:intergenic
GAGTTAGGAGAAAATTGTGG+AGG 0.641276 3:+91201038 MsaT015805.1:CDS
TCTTGTAAACAGCAAATGTG+AGG 0.641788 3:-91202145 None:intergenic
ATTCAGAGCCTTCAGTCACG+AGG 0.647455 3:+91204312 MsaT015805.1:CDS
ATCTGGTAAGATAGAGCTCG+AGG 0.650296 3:+91203744 MsaT015805.1:CDS
GGAATGCTCTAAGTGTGGGA+TGG 0.650848 3:+91205823 MsaT015805.1:CDS
AGTTGAGATCAGAATAGACA+TGG 0.657095 3:-91201230 None:intergenic
GCTTCACTACAAAGAGCAGT+GGG 0.658145 3:+91205054 MsaT015805.1:CDS
TTCAGAGCCTTCAGTCACGA+GGG 0.661654 3:+91204313 MsaT015805.1:CDS
AATTTGAGAAGCAACAAGGG+TGG 0.662837 3:+91201788 MsaT015805.1:CDS
GAAATCTCAAGGAAAAGGAG+TGG 0.667475 3:+91203822 MsaT015805.1:CDS
GAGATCAGAACTCCAATGAA+CGG 0.671093 3:+91203494 MsaT015805.1:CDS
AGCTATACCATTAAACTCTG+AGG 0.672242 3:-91204671 None:intergenic
TAGAAGCCATCACTATGCTG+AGG 0.678727 3:+91205247 MsaT015805.1:CDS
AGCAAGAGAATCAGGCAAAG+GGG 0.689158 3:+91202073 MsaT015805.1:CDS
TTTGTCCACCGTAACCGATG+GGG 0.692151 3:+91204795 MsaT015805.1:CDS
GATACATCAGAACCATACAT+GGG 0.696609 3:-91202750 None:intergenic
TCTCGTGCGAAACAGTAGCA+AGG 0.699858 3:-91203475 None:intergenic
GGTGTGGTTTGTGTAAGCAG+TGG 0.702118 3:+91205222 MsaT015805.1:CDS
AAACATGCATCAAATTGATG+GGG 0.720884 3:-91205273 None:intergenic
TTCACTCCATTCATGCAGGT+AGG 0.724284 3:+91204442 MsaT015805.1:CDS
GTGGACGAGAAAGAACCAAG+AGG 0.763430 3:-91204337 None:intergenic
TCCATCTCGAGAAAACACGG+AGG 0.824559 3:+91204469 MsaT015805.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 91200917 91205919 91200917 ID=MsaG015805
Chr3 mRNA 91200917 91205919 91200917 ID=MsaT015805.1;Parent=MsaG015805
Chr3 exon 91200917 91201288 91200917 ID=MsaT015805.1.exon1;Parent=MsaT015805.1
Chr3 CDS 91200917 91201288 91200917 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91201691 91201972 91201691 ID=MsaT015805.1.exon2;Parent=MsaT015805.1
Chr3 CDS 91201691 91201972 91201691 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91202050 91202219 91202050 ID=MsaT015805.1.exon3;Parent=MsaT015805.1
Chr3 CDS 91202050 91202219 91202050 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91202632 91202844 91202632 ID=MsaT015805.1.exon4;Parent=MsaT015805.1
Chr3 CDS 91202632 91202844 91202632 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91203194 91203380 91203194 ID=MsaT015805.1.exon5;Parent=MsaT015805.1
Chr3 CDS 91203194 91203380 91203194 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91203473 91203524 91203473 ID=MsaT015805.1.exon6;Parent=MsaT015805.1
Chr3 CDS 91203473 91203524 91203473 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91203617 91203846 91203617 ID=MsaT015805.1.exon7;Parent=MsaT015805.1
Chr3 CDS 91203617 91203846 91203617 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91203961 91204059 91203961 ID=MsaT015805.1.exon8;Parent=MsaT015805.1
Chr3 CDS 91203961 91204059 91203961 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91204163 91204393 91204163 ID=MsaT015805.1.exon9;Parent=MsaT015805.1
Chr3 CDS 91204163 91204393 91204163 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91204433 91205077 91204433 ID=MsaT015805.1.exon10;Parent=MsaT015805.1
Chr3 CDS 91204433 91205077 91204433 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91205117 91205325 91205117 ID=MsaT015805.1.exon11;Parent=MsaT015805.1
Chr3 CDS 91205117 91205325 91205117 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Chr3 exon 91205709 91205919 91205709 ID=MsaT015805.1.exon12;Parent=MsaT015805.1
Chr3 CDS 91205709 91205919 91205709 ID=cds.MsaT015805.1;Parent=MsaT015805.1
Gene Sequence

>MsaT015805.1

ATGGACACAAAGATGGGATTTTTAGGAGATGGTGGAAAGATGTGGCTTAAATGTTGGGAGAAGATCTTGGGAAGTACATGTAAAATCCATCAACAGTATTATCAGTGTATTGGTTCTAAAAGAGTTAGGAGAAAATTGTGGAGGAAATTGTTGCTGGCATGGGTTTTGGGTTGGTTTATAGTGTCTCTATGGATCTTTTGTGGTATTAACTTACACAACACTGAAAAGAGGAAAGAGACTTTAGCTAGCATGTGTGATGAAAGAGCTAGGATGTTACAAGATCAGTTTAATGTTAGTATGAATCATATTCAAGCCATGTCTATTCTGATCTCAACTTTCCATCATGCTAAGAATCCTTCTGCCATTGACCAGAAAACTTTTGCAAAGTATACAGAAAGAACTGCTTTTGAGAGGCCACTTACAAGTGGTGTTGCATATGCTGTAAGGGTGCTTCAATCTGAAAGGGAACAATTTGAGAAGCAACAAGGGTGGAGTATTAAGAGGATGGATACCATGGAGCAGAACCCGGTTCATGAGGATGATTATGTACCAGATGAATTGGAACCATCCCCTATCCATGAAGAATATGCTCCTGTCATCTTTGCACAAGATACAATTTCACATGTCATATCTATTGATGTGCTTTCTGGAAAGGAAGACCGTAAAAATATGTTGCGAGCAAGAGAATCAGGCAAAGGGGTTTTAACTGCCCCTTTTAGGCTGTTGAAAACAAACCATTTAGGAGTTATCCTCACATTTGCTGTTTACAAGAGAGATCTTCCATCAAATGCTACCCCAAATGAAAGGATTCAAGCAACTGATGGGTATCTTGGTGGGGTGTTTGAAATAGAGTCATTAGTGGAGAAATTGCTTCAGCAACTTGCTAGCAAGCAAACCGTGCTTGTTAATGTGTATGATACAACAAATCAGACACACCCAATTCCCATGTATGGTTCTGATGTATCAGGTGACGAGTTCTATCATGTGAGCTTTCTTAACTTTGGAGATCCTTTCAGGAAGCATGAGATGCACTGCAGGTTCAAACAGAAACCACCATTTCCAAGGTTGGCGACGGGTACATCAATTGGCATCCTTGTTATTGCACTCCTTGTTGGACATATTTGTCATGCAACTGTGAGTCGAATTGCCAAAGTAGAGGATGATTACCAAGAGATGATGGTGCTTAAAAAACGAGCTGAAGCAGCTGATGTTGCTAAATCCCAGTTCCTTGCTACTGTTTCGCACGAGATCAGAACTCCAATGAACGGTGTTTTAGGGATGATGAATATGCTCATGGACACTGATCTAGATGTAACCCAACAAGAATATGTCAGAACCGCACAAGGCAGTGGAAAAGCTCTGGTGTCAATTATAAATGAAGTTTTGGACCAGGCAAAGATTGAATCTGGTAAGATAGAGCTCGAGGCTGTGCTGTTCGATATACGAGCAATTATGGACGATGTGTTATCGCTATTTTCTGAGAAATCTCAAGGAAAAGGAGTGGAGTTAGCAGTTTATGTCTCGGATCAGGTTCCTCAACAGCTTATAGGTGATCCTGGAAGGTTTCGACAAATAATTACAAATCTCATGGGTAACTCGATTAAGTTCACAGACAAAGGACATATCTTTGTGACTATCCATCTTGTTGAGGAGGTTTTCCATTCAATAGAAGTTGAGAGAGAATCTACTTCAAAAGATGCAGAACATACATTAAGTGGTTTCCCAGTTGCTGATGGTCGCCGCAGCTGGGAAGGATTCAGAGCCTTCAGTCACGAGGGACCTCTTGGTTCTTTCTCGTCCACCTCCTCCAATGATCTCATCAGTTTGATTGTATCGCCGATGATATTCACTCCATTCATGCAGGTAGGTCCATCCATCTCGAGAAAACACGGAGGAACTGGTATTGGTCTAAGCATTAACAAGTGTTTAGTGGGCCTCATGAATGGAGAAATCAGATTTGTGGGCGAACCGAAGATTGGAAGCACTTTAACATTTACAGCTGTGTTCACCAATGGATGTCCTAATTCAAACGAGCTTCAAACTCAACAAATCAATAACCTACCTCATCCTGCAACCTCAGAGTTTAATGGTATAGCTGCACTAGTTATTGATCCGAGACCCGTTCGTGCTGAAGTGTCGAGATATCACATACAAAGGCTTGGCGTTCGTGTTGAAATAGTTTCAGATTTGAAGCAAAGTTTGTCCACCGTAACCGATGGGGATGCATCTATCAATATGGTCCTGATTGAACAAGAGGTTTGGGAAAGAGACTCAAGTATTTCATCGCACTTTGTGAATAACATTAGGAAAGTTGTTGAAGTTGATAAAGGAAAAGAGATTCCTCCAAAACTATTTATTCTCGTTAATTCTAGTATTTCTTTTAGAGCGAGTTCTACGGCATCATGTCTTCATAATCCAACAGGTGTCACAAAACCTTTGAGAGCAAGTATGCTTGCGGCTTCACTACAAAGAGCAGTGGGTGGTTTGTCTCTCAAGCATCTTCTCTTTGGGAGAAAGATTCTTATTGTAGATGATAATAGTGTGAATCGCACGGTAGCAGCTGGTGCTTTGAAAAAATATGGAGCTGGTGTGGTTTGTGTAAGCAGTGGAATAGAAGCCATCACTATGCTGAGGCCACCCCATCAATTTGATGCATGTTTCATGGATATTCAAATGCCTGAAATGGATGGGTTTGAAGCTACGCGTCGAATTCGAGAGATCGAAAACAATGTGAAAGACAGAGAACTGTTTGTTCATCTACCCATTTTGGCTATGACCGCAGACGTGATGCAGGCTACACACCAGGAATGCTCTAAGTGTGGGATGGATGGATATGTTTCAAAACCATTTGAAGCTGAACAACTTTATAGAGAGGTTTCAAAGTTTTTCCAATCATCTTGA
Protein sequence

>MsaT015805.1

MDTKMGFLGDGGKMWLKCWEKILGSTCKIHQQYYQCIGSKRVRRKLWRKLLLAWVLGWFIVSLWIFCGINLHNTEKRKETLASMCDERARMLQDQFNVSMNHIQAMSILISTFHHAKNPSAIDQKTFAKYTERTAFERPLTSGVAYAVRVLQSEREQFEKQQGWSIKRMDTMEQNPVHEDDYVPDELEPSPIHEEYAPVIFAQDTISHVISIDVLSGKEDRKNMLRARESGKGVLTAPFRLLKTNHLGVILTFAVYKRDLPSNATPNERIQATDGYLGGVFEIESLVEKLLQQLASKQTVLVNVYDTTNQTHPIPMYGSDVSGDEFYHVSFLNFGDPFRKHEMHCRFKQKPPFPRLATGTSIGILVIALLVGHICHATVSRIAKVEDDYQEMMVLKKRAEAADVAKSQFLATVSHEIRTPMNGVLGMMNMLMDTDLDVTQQEYVRTAQGSGKALVSIINEVLDQAKIESGKIELEAVLFDIRAIMDDVLSLFSEKSQGKGVELAVYVSDQVPQQLIGDPGRFRQIITNLMGNSIKFTDKGHIFVTIHLVEEVFHSIEVERESTSKDAEHTLSGFPVADGRRSWEGFRAFSHEGPLGSFSSTSSNDLISLIVSPMIFTPFMQVGPSISRKHGGTGIGLSINKCLVGLMNGEIRFVGEPKIGSTLTFTAVFTNGCPNSNELQTQQINNLPHPATSEFNGIAALVIDPRPVRAEVSRYHIQRLGVRVEIVSDLKQSLSTVTDGDASINMVLIEQEVWERDSSISSHFVNNIRKVVEVDKGKEIPPKLFILVNSSISFRASSTASCLHNPTGVTKPLRASMLAASLQRAVGGLSLKHLLFGRKILIVDDNSVNRTVAAGALKKYGAGVVCVSSGIEAITMLRPPHQFDACFMDIQMPEMDGFEATRRIREIENNVKDRELFVHLPILAMTADVMQATHQECSKCGMDGYVSKPFEAEQLYREVSKFFQSS*