AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015832


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015832 MtrunA17_Chr3g0122091 90.885 373 18 3 1 371 53 411 0.0 664
MsaG015832 MtrunA17_Chr1g0151261 46.185 249 130 3 44 292 50 294 2.67e-63 206
MsaG015832 MtrunA17_Chr1g0153141 33.203 256 138 8 37 284 43 273 1.42e-26 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015832 AT4G12240.1 56.977 344 126 5 38 371 30 361 3.51e-135 390
MsaG015832 AT5G52010.1 46.400 250 128 4 42 291 71 314 6.52e-63 206

Find 91 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGCTATTCTGAGCTTAT+TGG 0.177466 3:-91455036 None:intergenic
AGCTGAAACGGGCAGGGTTT+TGG 0.228799 3:+91455432 MsaT015832.1:CDS
TATGTAGAGTAGGGCTGTTC+TGG 0.246065 3:+91454967 MsaT015832.1:CDS
AGAATAGCTGCAGCTTCTTT+TGG 0.246225 3:+91455047 MsaT015832.1:CDS
TGTCTCAAGACTGTTGTTAT+TGG 0.251511 3:+91455605 MsaT015832.1:CDS
GTCTCAAGACTGTTGTTATT+GGG 0.278322 3:+91455606 MsaT015832.1:CDS
AGGGAGTAGAAAGGTGAAAT+TGG 0.313386 3:+91455322 MsaT015832.1:CDS
TGAGACGACACTGTCATTCT+TGG 0.314624 3:+91454904 MsaT015832.1:CDS
GAGACGACACTGTCATTCTT+GGG 0.319372 3:+91454905 MsaT015832.1:CDS
GGGTAAGTATGCTATGAAAA+TGG 0.326589 3:+91455346 MsaT015832.1:CDS
TTCATAAGGTGGGATTGAAT+TGG 0.333130 3:-91455009 None:intergenic
TTGGGGTCACGGAAGGATTT+CGG 0.339709 3:+91454923 MsaT015832.1:CDS
TCATAGGCGGGTTGAGTGTT+TGG 0.351038 3:+91455526 MsaT015832.1:CDS
AGCTTATTGGCAACTTCATA+AGG 0.365446 3:-91455023 None:intergenic
ACAGGAACTGTTGTATCGTT+TGG 0.373770 3:+91455151 MsaT015832.1:CDS
CTGTATCGGTTGTGGAAAAC+TGG 0.377175 3:+91455717 MsaT015832.1:CDS
GTTGTATCGTTTGGAGGATA+AGG 0.381598 3:+91455160 MsaT015832.1:CDS
CTCATGTTTGTCGTGTTTGT+GGG 0.391232 3:+91455204 MsaT015832.1:CDS
GGGTTCAAACTGTGTTAGAT+AGG 0.398462 3:+91455453 MsaT015832.1:CDS
CCTCATGTTTGTCGTGTTTG+TGG 0.413883 3:+91455203 MsaT015832.1:CDS
GCCAAACCATACCCAACTTT+AGG 0.418960 3:-91455404 None:intergenic
TCCTAAAGTTGGGTATGGTT+TGG 0.425982 3:+91455403 MsaT015832.1:CDS
TTGACTCCTAAAGTTGGGTA+TGG 0.429889 3:+91455398 MsaT015832.1:CDS
TCATAAGGTGGGATTGAATT+GGG 0.443856 3:-91455008 None:intergenic
TTTGATGGGGAAGGCGAAGA+AGG 0.453176 3:+91455691 MsaT015832.1:CDS
GATGCAGTTCCTAAAACATC+TGG 0.457052 3:+91455815 MsaT015832.1:CDS
AAATTAGACTCCGATGATAA+TGG 0.457209 3:+91455908 MsaT015832.1:CDS
TTGTATCGTTTGGAGGATAA+GGG 0.462961 3:+91455161 MsaT015832.1:CDS
GCTGATGAGCTGAAACGGGC+AGG 0.464292 3:+91455425 MsaT015832.1:CDS
TGGTGGATATGATGGATCAT+AGG 0.472905 3:+91455510 MsaT015832.1:CDS
TCGTGATATATTGAAAAGAT+TGG 0.474466 3:+91455745 MsaT015832.1:CDS
ATTGCTAAAAGTGTGGCTAT+TGG 0.475645 3:-91455096 None:intergenic
TGAGTGTTTGGTTCTTGTAT+CGG 0.489683 3:+91455538 MsaT015832.1:CDS
TCTCAAGACTGTTGTTATTG+GGG 0.494985 3:+91455607 MsaT015832.1:CDS
TATTGCATTGCAAAAGCATA+TGG 0.499781 3:+91455490 MsaT015832.1:CDS
CACTGTCATTCTTGGGGTCA+CGG 0.505218 3:+91454912 MsaT015832.1:CDS
ACTCTACATAGCTTCTCATT+CGG 0.506498 3:-91454954 None:intergenic
GAAGGAGGCTGTATCGGTTG+TGG 0.514904 3:+91455709 MsaT015832.1:CDS
GCAGTGATGGAGTCTTGAAA+AGG 0.516098 3:+91455636 MsaT015832.1:CDS
ATGTAGAGTAGGGCTGTTCT+GGG 0.518539 3:+91454968 MsaT015832.1:CDS
GTTTGGCTGATGAGCTGAAA+CGG 0.521781 3:+91455420 MsaT015832.1:CDS
GGCGAAGAAGGAGGCTGTAT+CGG 0.522314 3:+91455703 MsaT015832.1:CDS
AAAGCATATGGTGGATATGA+TGG 0.523680 3:+91455502 MsaT015832.1:CDS
GTCATTCTTGGGGTCACGGA+AGG 0.525218 3:+91454916 MsaT015832.1:CDS
GGAACTGTTGTATCGTTTGG+AGG 0.537669 3:+91455154 MsaT015832.1:CDS
GAAAATGGAAAAGTATAAGA+AGG 0.542297 3:+91455361 MsaT015832.1:CDS
TCGTGAGAGGAGGAAAGAAC+AGG 0.542932 3:+91455133 MsaT015832.1:CDS
CTCCTCTCACGAACAACGTG+GGG 0.546733 3:-91455122 None:intergenic
AATGAGAAGCTATGTAGAGT+AGG 0.549536 3:+91454957 MsaT015832.1:CDS
AAGGCGGAATCAGATAGAAT+CGG 0.553848 3:+91455295 MsaT015832.1:CDS
TATTGGCAACTTCATAAGGT+GGG 0.553988 3:-91455019 None:intergenic
CTATTGTGTAGAATATTCTA+CGG 0.555084 3:-91454851 None:intergenic
TTTGGCTGATGAGCTGAAAC+GGG 0.555204 3:+91455421 MsaT015832.1:CDS
ACAAGGATGACAGAGGTCCT+TGG 0.556458 3:+91455882 MsaT015832.1:CDS
TGGAGTTGTTCGACATATGG+TGG 0.559762 3:+91455067 MsaT015832.1:CDS
ATGAGAAGCTATGTAGAGTA+GGG 0.561703 3:+91454958 MsaT015832.1:CDS
CTGATGAGCTGAAACGGGCA+GGG 0.566688 3:+91455426 MsaT015832.1:CDS
ATCGGCTAGAGGGAGTAGAA+AGG 0.569230 3:+91455313 MsaT015832.1:CDS
AGAATATTCTACGGAACTTG+AGG 0.572133 3:-91454842 None:intergenic
GGATATGATGGATCATAGGC+GGG 0.572337 3:+91455514 MsaT015832.1:CDS
GAGTAGAAAGGTGAAATTGG+TGG 0.573655 3:+91455325 MsaT015832.1:CDS
ATCAGATAGAATCGGCTAGA+GGG 0.575206 3:+91455303 MsaT015832.1:CDS
ATTTGCAACACCATTATCAT+CGG 0.578456 3:-91455918 None:intergenic
ATGAGCGTGAGCATGCGAAA+AGG 0.580807 3:+91455276 MsaT015832.1:CDS
TAAGGTGGGATTGAATTGGG+TGG 0.598304 3:-91455005 None:intergenic
TCCTCCTCTCACGAACAACG+TGG 0.601028 3:-91455124 None:intergenic
AACAAATGTTTGAATGCCAG+TGG 0.602266 3:-91454877 None:intergenic
AGAATGACAGTGTCGTCTCA+AGG 0.604787 3:-91454901 None:intergenic
CCCACGTTGTTCGTGAGAGG+AGG 0.604916 3:+91455123 MsaT015832.1:CDS
AATGCAATATCCGCAGCTTG+CGG 0.606975 3:-91455476 None:intergenic
AGACGACACTGTCATTCTTG+GGG 0.607766 3:+91454906 MsaT015832.1:CDS
AGTAGAAAGGTGAAATTGGT+GGG 0.609374 3:+91455326 MsaT015832.1:CDS
TGCATTGCAAAAGCATATGG+TGG 0.610349 3:+91455493 MsaT015832.1:CDS
TTCTACACAATAGAAGCCAC+TGG 0.610989 3:+91454861 MsaT015832.1:CDS
GTGGACATATAACCCAGAAG+TGG 0.612100 3:+91455769 MsaT015832.1:CDS
TTATTGGCAACTTCATAAGG+TGG 0.612373 3:-91455020 None:intergenic
CCTCCTCTCACGAACAACGT+GGG 0.620111 3:-91455123 None:intergenic
GATGGGGAAGGCGAAGAAGG+AGG 0.621967 3:+91455694 MsaT015832.1:CDS
AATCAGATAGAATCGGCTAG+AGG 0.623094 3:+91455302 MsaT015832.1:CDS
ATGTTTGTCGTGTTTGTGGG+AGG 0.623967 3:+91455207 MsaT015832.1:CDS
AGCGTGAGCATGCGAAAAGG+CGG 0.626856 3:+91455279 MsaT015832.1:CDS
AGGATGACAGAGGTCCTTGG+TGG 0.631079 3:+91455885 MsaT015832.1:CDS
GTTAGATAGGCCGCAAGCTG+CGG 0.632475 3:+91455466 MsaT015832.1:CDS
TCATATTGATGATGACTACA+AGG 0.634259 3:+91455865 MsaT015832.1:CDS
TTATGAAGGAAGCAAAGCTA+CGG 0.640003 3:+91455582 MsaT015832.1:CDS
TGGATATGATGGATCATAGG+CGG 0.652070 3:+91455513 MsaT015832.1:CDS
ATATATTGAAAAGATTGGAG+TGG 0.659405 3:+91455750 MsaT015832.1:CDS
CCACAAACACGACAAACATG+AGG 0.661362 3:-91455203 None:intergenic
TCCTCTCACGAACAACGTGG+GGG 0.698129 3:-91455121 None:intergenic
TCGGAGTCTAATTTCCACCA+AGG 0.709650 3:-91455899 None:intergenic
GATGACTACAAGGATGACAG+AGG 0.730694 3:+91455875 MsaT015832.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 91454831 91455946 91454831 ID=MsaG015832
Chr3 mRNA 91454831 91455946 91454831 ID=MsaT015832.1;Parent=MsaG015832
Chr3 exon 91454831 91455946 91454831 ID=MsaT015832.1.exon1;Parent=MsaT015832.1
Chr3 CDS 91454831 91455946 91454831 ID=cds.MsaT015832.1;Parent=MsaT015832.1
Gene Sequence

>MsaT015832.1

ATGGTTTCAATCCTCAAGTTCCGTAGAATATTCTACACAATAGAAGCCACTGGCATTCAAACATTTGTTGCCTTGAGACGACACTGTCATTCTTGGGGTCACGGAAGGATTTCGGATAAAAATCCGAATGAGAAGCTATGTAGAGTAGGGCTGTTCTGGGACTTAGACAACAAACCACCCAATTCAATCCCACCTTATGAAGTTGCCAATAAGCTCAGAATAGCTGCAGCTTCTTTTGGAGTTGTTCGACATATGGTGGCTTATGCCAATAGCCACACTTTTAGCAATGTCCCCCACGTTGTTCGTGAGAGGAGGAAAGAACAGGAACTGTTGTATCGTTTGGAGGATAAGGGTGTGATTAAGCGTAATGAGCCTCATGTTTGTCGTGTTTGTGGGAGGAAATTTTACACGAATGAAAAGCTTGTTAATCATTTCAAGCAATTGCATGAGCGTGAGCATGCGAAAAGGCGGAATCAGATAGAATCGGCTAGAGGGAGTAGAAAGGTGAAATTGGTGGGTAAGTATGCTATGAAAATGGAAAAGTATAAGAAGGCTGCAAGTGCTGTTTTGACTCCTAAAGTTGGGTATGGTTTGGCTGATGAGCTGAAACGGGCAGGGTTTTGGGTTCAAACTGTGTTAGATAGGCCGCAAGCTGCGGATATTGCATTGCAAAAGCATATGGTGGATATGATGGATCATAGGCGGGTTGAGTGTTTGGTTCTTGTATCGGATGATTCTGATTTTGTCGATGTTATGAAGGAAGCAAAGCTACGGTGTCTCAAGACTGTTGTTATTGGGGATTTTAGCAGTGATGGAGTCTTGAAAAGGACTGCCGATACTGCTTTTTCTTGGGAGGATATTTTGATGGGGAAGGCGAAGAAGGAGGCTGTATCGGTTGTGGAAAACTGGAAAGATCGTGATATATTGAAAAGATTGGAGTGGACATATAACCCAGAAGTGGATAAAAAGAAACACAACTTGGATGATGCAGTTCCTAAAACATCTGGAGTTGATGATATTGAAGATATTTGTGATCATATTGATGATGACTACAAGGATGACAGAGGTCCTTGGTGGAAATTAGACTCCGATGATAATGGTGTTGCAAATCGATAA

Protein sequence

>MsaT015832.1

MVSILKFRRIFYTIEATGIQTFVALRRHCHSWGHGRISDKNPNEKLCRVGLFWDLDNKPPNSIPPYEVANKLRIAAASFGVVRHMVAYANSHTFSNVPHVVRERRKEQELLYRLEDKGVIKRNEPHVCRVCGRKFYTNEKLVNHFKQLHEREHAKRRNQIESARGSRKVKLVGKYAMKMEKYKKAASAVLTPKVGYGLADELKRAGFWVQTVLDRPQAADIALQKHMVDMMDHRRVECLVLVSDDSDFVDVMKEAKLRCLKTVVIGDFSSDGVLKRTADTAFSWEDILMGKAKKEAVSVVENWKDRDILKRLEWTYNPEVDKKKHNLDDAVPKTSGVDDIEDICDHIDDDYKDDRGPWWKLDSDDNGVANR*