Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015842 | XP_024633575.1 | 73.256 | 258 | 55 | 3 | 1 | 246 | 1 | 256 | 6.28e-108 | 322 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015842 | tr|G7JBB9|G7JBB9_MEDTR | 75.391 | 256 | 23 | 4 | 1 | 243 | 1 | 229 | 1.66e-97 | 293 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG015842 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015842 | MtrunA17_Chr3g0122071 | 73.047 | 256 | 22 | 4 | 1 | 243 | 1 | 222 | 6.46e-96 | 280 |
| MsaG015842 | MtrunA17_Chr4g0064011 | 56.855 | 248 | 89 | 8 | 1 | 246 | 1 | 232 | 1.53e-71 | 218 |
| MsaG015842 | MtrunA17_Chr6g0474091 | 45.113 | 133 | 60 | 6 | 1 | 133 | 1 | 120 | 1.91e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015842 | AT2G18490.1 | 55.714 | 70 | 31 | 0 | 122 | 191 | 142 | 211 | 2.42e-20 | 87.4 |
| MsaG015842 | AT2G18490.2 | 55.714 | 70 | 31 | 0 | 122 | 191 | 192 | 261 | 3.21e-20 | 87.8 |
| MsaG015842 | AT2G15740.1 | 51.899 | 79 | 34 | 1 | 122 | 200 | 217 | 291 | 2.83e-19 | 85.5 |
| MsaG015842 | AT5G22990.1 | 50.602 | 83 | 40 | 1 | 135 | 216 | 232 | 314 | 2.23e-18 | 83.2 |
| MsaG015842 | AT5G42640.1 | 42.424 | 99 | 53 | 1 | 93 | 191 | 191 | 285 | 3.11e-18 | 82.4 |
Find 29 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGTCTTGAAGCACAATT+TGG | 0.219378 | 3:+91595793 | MsaT015842.1:CDS |
| TCAGTTGGTACCAATAATTT+TGG | 0.290051 | 3:+91595739 | MsaT015842.1:CDS |
| AGCATGACTACCAAAATTAT+TGG | 0.316188 | 3:-91595749 | None:intergenic |
| AGCCTACCACACAAGAAATA+TGG | 0.358104 | 3:+91596096 | MsaT015842.1:CDS |
| TATTAATAATAATTATTTGA+AGG | 0.406891 | 3:-91596405 | None:intergenic |
| TTATCAGCACAAGCAATAAC+AGG | 0.430056 | 3:-91596300 | None:intergenic |
| ACAATCATAGATTGGTTCAA+TGG | 0.452424 | 3:+91595683 | MsaT015842.1:CDS |
| TTCTAGGATTCGAAAGAGAC+CGG | 0.465129 | 3:+91596233 | MsaT015842.1:CDS |
| GATAAGCCTACATGGAGATC+AGG | 0.469377 | 3:-91595946 | None:intergenic |
| AGCAATGAAAATATTGATGA+TGG | 0.472887 | 3:+91596063 | MsaT015842.1:CDS |
| AGAGTCTTGAAGCACAATTT+GGG | 0.488266 | 3:+91595794 | MsaT015842.1:CDS |
| ATTTCTTGTGTGGTAGGCTG+TGG | 0.491007 | 3:-91596092 | None:intergenic |
| TAATATGAACAATCATAGAT+TGG | 0.492110 | 3:+91595675 | None:intergenic |
| TCACATCCTGATCTCCATGT+AGG | 0.522755 | 3:+91595940 | MsaT015842.1:CDS |
| ACCAACTGATGATGATGAAT+GGG | 0.541850 | 3:-91595725 | None:intergenic |
| GTCCATATTTCTTGTGTGGT+AGG | 0.551362 | 3:-91596098 | None:intergenic |
| CTCTCACCCCTCTTCCAAGT+GGG | 0.558684 | 3:+91596364 | MsaT015842.1:CDS |
| TACCAACTGATGATGATGAA+TGG | 0.562352 | 3:-91595726 | None:intergenic |
| CAAATTCAAAAGCTCAATGA+CGG | 0.582932 | 3:+91596261 | MsaT015842.1:CDS |
| AGATTGGTTCAATGGATGCA+TGG | 0.584866 | 3:+91595691 | MsaT015842.1:CDS |
| GCTCTCACCCCTCTTCCAAG+TGG | 0.584935 | 3:+91596363 | MsaT015842.1:CDS |
| TATGGTCCATATTTCTTGTG+TGG | 0.586966 | 3:-91596102 | None:intergenic |
| ACCCATTCATCATCATCAGT+TGG | 0.590463 | 3:+91595724 | MsaT015842.1:CDS |
| TCTAGGATTCGAAAGAGACC+GGG | 0.623769 | 3:+91596234 | MsaT015842.1:CDS |
| TCCGGGAATCATAAAAGCGA+TGG | 0.628214 | 3:+91595771 | MsaT015842.1:CDS |
| ATTGTGATGATAAGCCTACA+TGG | 0.633345 | 3:-91595954 | None:intergenic |
| AAGTGGGATAAAAGTCAAGT+CGG | 0.648453 | 3:+91596380 | MsaT015842.1:CDS |
| GACGACGACGATGTTGATGT+AGG | 0.650410 | 3:-91595823 | None:intergenic |
| ATGTTAACAAGAACGATACG+AGG | 0.702248 | 3:+91596028 | MsaT015842.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 91595679 | 91596425 | 91595679 | ID=MsaG015842 |
| Chr3 | mRNA | 91595679 | 91596425 | 91595679 | ID=MsaT015842.1;Parent=MsaG015842 |
| Chr3 | exon | 91595679 | 91596425 | 91595679 | ID=MsaT015842.1.exon1;Parent=MsaT015842.1 |
| Chr3 | CDS | 91595679 | 91596425 | 91595679 | ID=cds.MsaT015842.1;Parent=MsaT015842.1 |
| Gene Sequence |
| Protein sequence |
