AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015867

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015867 MtrunA17_Chr3g0122351 94.561 956 29 1 1 933 1 956 0.0 1885
MsaG015867 MtrunA17_Chr8g0377501 58.435 984 318 14 1 919 8 965 0.0 1066
MsaG015867 MtrunA17_Chr4g0068161 30.353 962 545 29 8 912 26 919 1.18e-114 374
MsaG015867 MtrunA17_Chr2g0279141 36.705 613 300 13 375 919 439 1031 4.44e-111 367
MsaG015867 MtrunA17_Chr2g0279141 49.721 179 82 3 4 175 10 187 1.30e-43 171
MsaG015867 MtrunA17_Chr8g0382191 37.205 551 287 11 386 888 501 1040 5.76e-100 337
MsaG015867 MtrunA17_Chr8g0382191 48.521 169 83 2 6 171 8 175 1.80e-43 171
MsaG015867 MtrunA17_Chr4g0050401 35.875 577 289 15 375 888 426 984 4.93e-97 328
MsaG015867 MtrunA17_Chr4g0050401 46.497 157 81 1 4 157 10 166 4.16e-38 154
MsaG015867 MtrunA17_Chr2g0294711 30.511 567 318 15 375 885 340 886 7.43e-71 253
MsaG015867 MtrunA17_Chr2g0294711 52.703 148 68 1 8 155 26 171 1.05e-40 162
MsaG015867 MtrunA17_Chr8g0382181 29.630 486 261 15 48 476 7 468 1.21e-46 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015867 AT1G67310.1 46.450 1014 426 24 5 932 34 1016 0.0 806
MsaG015867 AT5G09410.4 35.831 614 316 15 379 928 281 880 8.00e-102 338
MsaG015867 AT5G64220.1 37.567 559 288 14 379 886 451 999 2.69e-101 340
MsaG015867 AT5G64220.1 47.879 165 84 1 6 168 12 176 1.69e-43 172
MsaG015867 AT5G64220.2 37.567 559 288 14 379 886 451 999 2.69e-101 340
MsaG015867 AT5G64220.2 47.879 165 84 1 6 168 12 176 1.69e-43 172
MsaG015867 AT5G09410.2 35.889 613 317 15 379 928 402 1001 4.06e-101 339
MsaG015867 AT5G09410.2 51.825 137 66 0 1 137 10 146 8.05e-42 166
MsaG015867 AT5G09410.1 35.889 613 317 15 379 928 384 983 6.06e-101 338
MsaG015867 AT5G09410.1 51.825 137 66 0 1 137 10 146 9.62e-42 166
MsaG015867 AT5G09410.3 35.889 613 317 15 379 928 461 1060 2.23e-100 338
MsaG015867 AT5G09410.3 53.488 129 55 1 1 124 69 197 6.52e-38 154
MsaG015867 AT2G22300.3 35.766 548 294 11 379 888 396 923 2.75e-98 331
MsaG015867 AT2G22300.3 51.240 121 58 1 53 172 1 121 4.65e-31 132
MsaG015867 AT2G22300.2 35.766 548 294 11 379 888 454 981 4.42e-98 331
MsaG015867 AT2G22300.2 45.614 171 92 1 3 172 9 179 3.78e-42 167
MsaG015867 AT2G22300.1 35.766 548 294 11 379 888 454 981 4.42e-98 331
MsaG015867 AT2G22300.1 45.614 171 92 1 3 172 9 179 3.78e-42 167
MsaG015867 AT3G16940.3 30.864 567 343 13 373 901 262 817 3.12e-72 255
MsaG015867 AT3G16940.3 52.414 145 66 2 12 156 1 142 1.57e-39 158
MsaG015867 AT3G16940.1 30.864 567 343 13 373 901 289 844 5.40e-72 255
MsaG015867 AT3G16940.1 51.007 149 70 2 8 156 24 169 6.84e-40 159
MsaG015867 AT3G16940.2 28.478 460 284 10 373 796 289 739 2.65e-48 184
MsaG015867 AT3G16940.2 51.007 149 70 2 8 156 24 169 3.88e-40 159

Find 228 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTGTAAATTTCTGTTTC+TGG 0.128293 3:-91836071 None:intergenic
CATCCCGAGGTTCTTGAATT+TGG 0.192741 3:-91836026 None:intergenic
TATAATCCCTTGCTCTTTCT+TGG 0.193263 3:-91837375 None:intergenic
GGGATTATACACATAGTTTC+TGG 0.215272 3:+91837389 MsaT015867.1:CDS
AATTTCATGCTCATTATAAA+AGG 0.218703 3:-91831537 None:intergenic
TGAGTTTCTATTGAAGAATA+TGG 0.231372 3:-91835961 None:intergenic
GCCTCCACCACTTGTCTAAA+TGG 0.241617 3:+91838501 MsaT015867.1:CDS
TCATTGGCATAACAATATTC+TGG 0.250713 3:-91836105 None:intergenic
TTCGAAAAGATGGTCATAAT+TGG 0.253911 3:+91829455 MsaT015867.1:CDS
AATGGATATGTCACCGGTTT+AGG 0.257839 3:+91838519 MsaT015867.1:CDS
TTGCATTCACTGTAATGTTT+TGG 0.270778 3:+91837058 MsaT015867.1:CDS
CAGTCCCTTGTGCCCATTGC+TGG 0.272292 3:-91838218 None:intergenic
GAAACGCCTCTATCTTCTTT+TGG 0.274448 3:+91835732 MsaT015867.1:CDS
TTGAAGAATATGGAGAAAAT+TGG 0.278554 3:-91835951 None:intergenic
TTCTCATCATTATTGCTGTT+GGG 0.280715 3:-91835798 None:intergenic
GTTTCCAGAGGCGAAGTTAT+TGG 0.289983 3:+91830206 MsaT015867.1:CDS
CAACCATATGATGGATATAA+TGG 0.292630 3:+91831593 MsaT015867.1:CDS
TCTACCTGGGCTTGTATGTT+TGG 0.292828 3:+91836879 MsaT015867.1:CDS
GTGCATTACTTCAGGAAATA+AGG 0.299775 3:+91836986 MsaT015867.1:CDS
CCATTTGGATCTTGTGAACT+TGG 0.310113 3:-91838172 None:intergenic
GAGGCTCCGTCCCATCTTCT+TGG 0.324091 3:+91836951 MsaT015867.1:CDS
TCAAGCTCAGAAGAATCTTT+AGG 0.325777 3:-91838310 None:intergenic
GGCAATACAAGCTAATGATA+TGG 0.326426 3:+91838909 MsaT015867.1:CDS
CTAGGAGAGCATCTATAATA+TGG 0.330922 3:-91837229 None:intergenic
TGCATCTTCATCTCGTTCAT+TGG 0.331556 3:-91837339 None:intergenic
AGTTCCTGCTGAAATAATTC+AGG 0.333245 3:+91836911 MsaT015867.1:CDS
ATAACAGTTCTTCTGGACTA+TGG 0.334037 3:-91837094 None:intergenic
CACAACTGAGAATGGGGTTC+AGG 0.337492 3:-91837430 None:intergenic
GCATCTATTGCGGCCAGCAA+TGG 0.343835 3:+91838205 MsaT015867.1:CDS
CTTCATTGGGCTGCACGGTT+TGG 0.345093 3:+91837494 MsaT015867.1:CDS
ACAAGTAATAACAGTTCTTC+TGG 0.345367 3:-91837101 None:intergenic
CCTCTCTGCTTCGATTATAA+AGG 0.347695 3:+91837142 MsaT015867.1:CDS
AACTTAATTGTTCCTCCAAT+TGG 0.352792 3:-91831484 None:intergenic
GCCTCCCCTCGGATTCTACC+TGG 0.353045 3:+91836865 MsaT015867.1:CDS
GTTGGGGTGTATCCGGTAAC+TGG 0.354213 3:-91835781 None:intergenic
CGCACAAGCTCTGCGCCAAT+TGG 0.355249 3:+91831469 MsaT015867.1:CDS
TCCTTGTATGCGTGTTGTTT+TGG 0.357909 3:-91835306 None:intergenic
GGGAAAATCGGTGAACTTGC+AGG 0.359647 3:-91829256 None:intergenic
TATGGTTGTTTATGATCATC+TGG 0.363053 3:-91831578 None:intergenic
TTCACATGAATCACCAACTA+TGG 0.363538 3:-91831354 None:intergenic
CATCTATTGCGGCCAGCAAT+GGG 0.366299 3:+91838206 MsaT015867.1:CDS
GTTCTCATCATTATTGCTGT+TGG 0.366506 3:-91835799 None:intergenic
CTCCAGTCCTGGGTTCTTAG+AGG 0.368654 3:+91831391 MsaT015867.1:CDS
ATTGCTGCTGGTGCATCTGC+TGG 0.368996 3:+91838136 MsaT015867.1:CDS
GATATGGGCAGTTGGAATCT+TGG 0.378012 3:+91838925 MsaT015867.1:CDS
GTGGCATTGGTGGATATGTT+AGG 0.378824 3:+91838541 MsaT015867.1:CDS
ATGATTTATATCAATTTCCT+TGG 0.379049 3:+91839636 MsaT015867.1:CDS
ATGGATGTTGTCTCAGGGTT+TGG 0.382049 3:-91831335 None:intergenic
TGTTACCATGAGGCTGGTTA+AGG 0.382153 3:-91829278 None:intergenic
GTATCCGGTAACTGGTTTCC+TGG 0.383094 3:-91835773 None:intergenic
CTTGACCATGATTGCCCTGC+TGG 0.384698 3:+91835216 MsaT015867.1:CDS
GTTGCTCTGTGCATTACTTC+AGG 0.384723 3:+91836978 MsaT015867.1:CDS
GTTCCATTATATCCATCATA+TGG 0.384932 3:-91831596 None:intergenic
ATGGATGGACTGCCCTTCAT+TGG 0.385897 3:+91837480 MsaT015867.1:CDS
CCAACTATGGATGTTGTCTC+AGG 0.386456 3:-91831341 None:intergenic
CTTGTTTGCAAATGAGGACT+TGG 0.388891 3:-91835280 None:intergenic
TGCTAAGGTGGCGGAGAAAA+CGG 0.391461 3:+91838957 MsaT015867.1:CDS
GAGGTTCTTGAATTTGGCTT+TGG 0.394026 3:-91836020 None:intergenic
CATGTGTAGGTGATCATTGT+TGG 0.398565 3:+91836831 MsaT015867.1:intron
TCCACCAATGCCACCTAAAC+CGG 0.398700 3:-91838532 None:intergenic
GTTAATTTCCGTGATATCAA+TGG 0.399308 3:+91837461 MsaT015867.1:CDS
AGAAGCGCATGAACGGCTTA+AGG 0.406373 3:+91829507 MsaT015867.1:CDS
GCTTGTATTGCCTCCGAGTC+TGG 0.406666 3:-91838898 None:intergenic
TGGTAACTTGTTTGCAAATG+AGG 0.409912 3:-91835286 None:intergenic
ATACACATAGTTTCTGGATT+GGG 0.416266 3:+91837395 MsaT015867.1:CDS
GTGAACCTTTGTTGCCTCAT+TGG 0.418858 3:-91836121 None:intergenic
AGAAAGCAGATGATGATTCA+TGG 0.418980 3:+91837204 MsaT015867.1:CDS
GATGGATATAATGGAACAAA+AGG 0.419267 3:+91831602 MsaT015867.1:CDS
AATCAGAATTTCTCCAGTCC+TGG 0.422608 3:+91831380 MsaT015867.1:CDS
ATGCGTTTCTTTCGAAAAGA+TGG 0.423560 3:+91829445 MsaT015867.1:CDS
AAGGATGATAGCTCAGAATC+TGG 0.430655 3:+91837161 MsaT015867.1:CDS
TATTGCTGTTGGGGTGTATC+CGG 0.432086 3:-91835788 None:intergenic
TTACAGGGGACATCCAATTC+TGG 0.432848 3:+91831260 MsaT015867.1:intron
CAGCATCTCTGTCCAAGATA+AGG 0.435726 3:-91835250 None:intergenic
TGGATGGACTGCCCTTCATT+GGG 0.436126 3:+91837481 MsaT015867.1:CDS
GGACTGGAGAAATTCTGATT+AGG 0.437165 3:-91831377 None:intergenic
TGGCAACACGAGTGATGAAA+TGG 0.437603 3:+91839547 MsaT015867.1:CDS
TGCAGCAATTCAAAAGGCTT+TGG 0.437608 3:+91839072 MsaT015867.1:CDS
CTGCCCTTCATTGGGCTGCA+CGG 0.438440 3:+91837489 MsaT015867.1:CDS
CTTATCTTGGACAGAGATGC+TGG 0.440239 3:+91835251 MsaT015867.1:CDS
CATTGGCATAACAATATTCT+GGG 0.440294 3:-91836104 None:intergenic
CAGCAATGGGCACAAGGGAC+TGG 0.440405 3:+91838219 MsaT015867.1:CDS
GTTGCTTCACTTATTGCTGC+TGG 0.440412 3:+91838124 MsaT015867.1:CDS
ATTACAGTGAATGCAACTAT+TGG 0.441063 3:-91837051 None:intergenic
AAAATGTAAAGCACTTCTAT+AGG 0.441324 3:-91829219 None:intergenic
ATTCATGCCACAACTGAGAA+TGG 0.444168 3:-91837438 None:intergenic
CATCAGTATAATCGCATGCT+TGG 0.446008 3:+91839133 MsaT015867.1:CDS
ATATTTCTTCTGAATTGATA+AGG 0.446959 3:-91838589 None:intergenic
ATGAATTTACGTTGCCAACT+AGG 0.447557 3:-91837247 None:intergenic
TTCATGCCACAACTGAGAAT+GGG 0.447886 3:-91837437 None:intergenic
TCTGGTTCAGCGAATGCAAC+AGG 0.449281 3:-91831560 None:intergenic
CCAACCAGGAAACCAGTTAC+CGG 0.462646 3:+91835769 MsaT015867.1:CDS
CAACTTCTGTGGATGATGCT+TGG 0.463627 3:+91839582 MsaT015867.1:CDS
TATACACATAGTTTCTGGAT+TGG 0.464836 3:+91837394 MsaT015867.1:CDS
GTTTGGGCAGGCTCATGTGA+GGG 0.465902 3:+91838871 MsaT015867.1:intron
CAAACCGTGCAGCCCAATGA+AGG 0.466086 3:-91837493 None:intergenic
TTCAGAAGAAATATAGAGGC+TGG 0.466525 3:+91838598 MsaT015867.1:CDS
AAGAGAAATGATTGCCAACC+AGG 0.467341 3:+91835755 MsaT015867.1:CDS
GAGCTACGACTCTGACTAAA+CGG 0.468229 3:-91831302 None:intergenic
ATCCTTTCTCTGCCTCCCCT+CGG 0.469219 3:+91836854 MsaT015867.1:CDS
TCTCTTCCAAAAGAAGATAG+AGG 0.474103 3:-91835738 None:intergenic
TTCTGGATTGGGTTTCGAGT+GGG 0.474526 3:+91837406 MsaT015867.1:CDS
GAGCAACCTTTCCAAGAAGA+TGG 0.475271 3:-91836962 None:intergenic
ACAACTGAGAATGGGGTTCA+GGG 0.477876 3:-91837429 None:intergenic
ATCAGTATAATCGCATGCTT+GGG 0.478091 3:+91839134 MsaT015867.1:CDS
GCTAAGGTGGCGGAGAAAAC+GGG 0.481096 3:+91838958 MsaT015867.1:CDS
TCTCATCATTATTGCTGTTG+GGG 0.481799 3:-91835797 None:intergenic
TTCAAGAAGCTAAGAAAAGA+TGG 0.482062 3:+91829190 MsaT015867.1:CDS
GTTACCATGAGGCTGGTTAA+GGG 0.482952 3:-91829277 None:intergenic
CTTGGAATATTGATGATGCT+TGG 0.484389 3:+91839600 MsaT015867.1:CDS
CATGCCCAGCAGGGCAATCA+TGG 0.486673 3:-91835221 None:intergenic
GTTGGCAACGTAAATTCATC+TGG 0.487682 3:+91837251 MsaT015867.1:CDS
AGGCTGGTTAAGGGGAAAAT+CGG 0.489276 3:-91829268 None:intergenic
ATTATAGATGCTCTCCTAGT+TGG 0.490367 3:+91837233 MsaT015867.1:CDS
GCAATACAAGCTAATGATAT+GGG 0.490598 3:+91838910 MsaT015867.1:CDS
CCGGTAACTGGTTTCCTGGT+TGG 0.491923 3:-91835769 None:intergenic
ATCCGAGGGGAGGCAGAGAA+AGG 0.494394 3:-91836856 None:intergenic
TGAAGGATAAGCTGCGGCAC+TGG 0.500111 3:+91837303 MsaT015867.1:CDS
TCAATTCAGAAGAAATATAG+AGG 0.500680 3:+91838594 MsaT015867.1:CDS
ACATCCAATAACTTCGCCTC+TGG 0.501649 3:-91830210 None:intergenic
TTGACCATGATTGCCCTGCT+GGG 0.501670 3:+91835217 MsaT015867.1:CDS
ACAAGCTCTGCGCCAATTGG+AGG 0.502276 3:+91831472 MsaT015867.1:CDS
ATCAGAATTTCTCCAGTCCT+GGG 0.502563 3:+91831381 MsaT015867.1:CDS
AACTCTTTGATTTCACTGCA+TGG 0.505283 3:-91837011 None:intergenic
GAAAAGATGGTCATAATTGG+CGG 0.506642 3:+91829458 MsaT015867.1:CDS
TAGTGAATCCTCATCATCAC+TGG 0.508175 3:-91838377 None:intergenic
ATTTCATGCTCATTATAAAA+GGG 0.509625 3:-91831536 None:intergenic
CCATCCTGAATTATTTCAGC+AGG 0.510184 3:-91836915 None:intergenic
AGTACATGCAGCAATTCAAA+AGG 0.511198 3:+91839066 MsaT015867.1:CDS
ACCGGTTTAGGTGGCATTGG+TGG 0.512373 3:+91838531 MsaT015867.1:CDS
TCCATTTAGACAAGTGGTGG+AGG 0.513397 3:-91838502 None:intergenic
TTGTGTGACAGGACCAGAAT+TGG 0.513446 3:-91831273 None:intergenic
TTTGGCACGGGTGATTTCCA+TGG 0.514213 3:+91839090 MsaT015867.1:CDS
AATGGGCACAAGGGACTGGC+AGG 0.514534 3:+91838223 MsaT015867.1:CDS
GATCATAAACAACCATATGA+TGG 0.514783 3:+91831584 MsaT015867.1:CDS
TGATTTATATCAATTTCCTT+GGG 0.515469 3:+91839637 MsaT015867.1:CDS
TAACAGTTCTTCTGGACTAT+GGG 0.516224 3:-91837093 None:intergenic
AATTCAAAAGGCTTTGGCAC+GGG 0.518782 3:+91839078 MsaT015867.1:CDS
GGAACAATTAAGTTTGAATG+AGG 0.519133 3:+91831493 MsaT015867.1:CDS
AAGCTAATGATATGGGCAGT+TGG 0.520016 3:+91838917 MsaT015867.1:CDS
GTCACCGGTTTAGGTGGCAT+TGG 0.523450 3:+91838528 MsaT015867.1:CDS
TCCGTCCCATCTTCTTGGAA+AGG 0.524865 3:+91836956 MsaT015867.1:CDS
CTGAACCCCATTCTCAGTTG+TGG 0.525541 3:+91837431 MsaT015867.1:CDS
ATTGGGCTGCACGGTTTGGA+AGG 0.532088 3:+91837498 MsaT015867.1:CDS
GACCTCTAAGAACCCAGGAC+TGG 0.539550 3:-91831393 None:intergenic
AAGCCAAATTCAAGAACCTC+GGG 0.541578 3:+91836023 MsaT015867.1:CDS
ACCAAAACAACACGCATACA+AGG 0.542431 3:+91835305 MsaT015867.1:CDS
CCAAGTTCACAAGATCCAAA+TGG 0.543852 3:+91838172 MsaT015867.1:CDS
AACGGTGATGATTGTGTGAC+AGG 0.544242 3:-91831284 None:intergenic
TTTGGGCAGGCTCATGTGAG+GGG 0.545911 3:+91838872 MsaT015867.1:intron
CTGTTTCAGAAGCGCATGAA+CGG 0.548045 3:+91829500 MsaT015867.1:CDS
CCTCCCCTCGGATTCTACCT+GGG 0.548614 3:+91836866 MsaT015867.1:CDS
TGTCTAAATGGATATGTCAC+CGG 0.549024 3:+91838513 MsaT015867.1:CDS
AGTCATCATGAATAATGAAA+CGG 0.549592 3:+91831427 MsaT015867.1:CDS
TGCATCTGCTGGAGCAGTGA+CGG 0.550109 3:+91838147 MsaT015867.1:CDS
TGATGACGAGCATGCACAGA+AGG 0.550115 3:-91839115 None:intergenic
GGATTCACTAAGAAATACCT+TGG 0.550666 3:+91838390 MsaT015867.1:CDS
TAAGAATGTTACCATGAGGC+TGG 0.551040 3:-91829284 None:intergenic
TGCGGCCAGCAATGGGCACA+AGG 0.551436 3:+91838213 MsaT015867.1:CDS
TTACTTCCTCAAGACACTGG+TGG 0.551472 3:+91835925 MsaT015867.1:CDS
TGTTTGGGCAGGCTCATGTG+AGG 0.551502 3:+91838870 MsaT015867.1:intron
AACTTCGCCTCTGGAAACTA+CGG 0.556762 3:-91830201 None:intergenic
TTTCCCCTTAACCAGCCTCA+TGG 0.561765 3:+91829273 MsaT015867.1:CDS
GGATATGTCACCGGTTTAGG+TGG 0.565101 3:+91838522 MsaT015867.1:CDS
CCTGAGACAACATCCATAGT+TGG 0.569426 3:+91831341 MsaT015867.1:CDS
CAATTCAAAAGGCTTTGGCA+CGG 0.569867 3:+91839077 MsaT015867.1:CDS
CTAAACTGCTACTATGCACA+TGG 0.570887 3:+91830169 MsaT015867.1:CDS
AGATCAATTACGATGCTACC+TGG 0.572627 3:+91828357 None:intergenic
CAGTCCATCCATTGATATCA+CGG 0.574632 3:-91837469 None:intergenic
ATGTGAGGGGTTACCAGACT+CGG 0.574934 3:+91838885 MsaT015867.1:CDS
GCATATTCAAGCTTGACTGT+TGG 0.580162 3:+91836048 MsaT015867.1:CDS
TTTCTGGATTGGGTTTCGAG+TGG 0.582020 3:+91837405 MsaT015867.1:CDS
AGCAACCTTTCCAAGAAGAT+GGG 0.582697 3:-91836961 None:intergenic
AGAATATTGTTATGCCAATG+AGG 0.584614 3:+91836107 MsaT015867.1:CDS
AATGAACGAGATGAAGATGC+AGG 0.584800 3:+91837341 MsaT015867.1:CDS
TCTTTGTCCAAGAAAGAGCA+AGG 0.586979 3:+91837368 MsaT015867.1:CDS
TGGACAAGGTTGTGCTAAGG+TGG 0.588436 3:+91838945 MsaT015867.1:CDS
TTACCATGAGGCTGGTTAAG+GGG 0.590627 3:-91829276 None:intergenic
CTTTGTCCAAGAAAGAGCAA+GGG 0.591267 3:+91837369 MsaT015867.1:CDS
TCTTGAAGCCAGTGATGATG+AGG 0.591725 3:+91838369 MsaT015867.1:CDS
TGGGATGCGTCGTCGAGCCG+AGG 0.592717 3:+91839153 MsaT015867.1:CDS
TCTTCTTCAAGAGCTGCTGA+AGG 0.595191 3:+91837286 MsaT015867.1:CDS
ACCTTTCCAAGAAGATGGGA+CGG 0.596282 3:-91836957 None:intergenic
AAAGCCAAATTCAAGAACCT+CGG 0.596609 3:+91836022 MsaT015867.1:CDS
AACACGGTTATCAACTTCTG+TGG 0.597925 3:+91839571 MsaT015867.1:CDS
TTATGCCAATGAGGCAACAA+AGG 0.601144 3:+91836116 MsaT015867.1:CDS
GATTCACTAAGAAATACCTT+GGG 0.601371 3:+91838391 MsaT015867.1:CDS
AAACCGTGCAGCCCAATGAA+GGG 0.601471 3:-91837492 None:intergenic
GACATATCCATTTAGACAAG+TGG 0.602811 3:-91838508 None:intergenic
TCATCTGTCATCCCTCACAT+TGG 0.603114 3:+91838273 MsaT015867.1:CDS
CAACTATGGATGTTGTCTCA+GGG 0.603209 3:-91831340 None:intergenic
ATTTCCGTGATATCAATGGA+TGG 0.609222 3:+91837465 MsaT015867.1:CDS
CCCAGGTAGAATCCGAGGGG+AGG 0.609248 3:-91836866 None:intergenic
ACAAGGTTGTGCTAAGGTGG+CGG 0.609415 3:+91838948 MsaT015867.1:CDS
GTCACCAAACATACAAGCCC+AGG 0.609877 3:-91836883 None:intergenic
TGGACTCCACCAGTGTCTTG+AGG 0.610261 3:-91835931 None:intergenic
TAAAACTGCTGCATCTATTG+CGG 0.610279 3:+91838195 MsaT015867.1:CDS
ACAAGCCCAGGTAGAATCCG+AGG 0.613314 3:-91836871 None:intergenic
ATATCCATTTAGACAAGTGG+TGG 0.614907 3:-91838505 None:intergenic
ACATTCTTTCAGAAAACGGA+TGG 0.616326 3:+91838469 MsaT015867.1:intron
TCATGCTCATTATAAAAGGG+TGG 0.618544 3:-91831533 None:intergenic
ATTCTCTTACAGGCTGAACA+TGG 0.626364 3:+91839527 MsaT015867.1:intron
TCTTGGACAAGGTTGTGCTA+AGG 0.628079 3:+91838942 MsaT015867.1:CDS
GAAGGCTAGAGCGCTTCCCA+AGG 0.628496 3:-91839653 None:intergenic
CAAGCCCAGGTAGAATCCGA+GGG 0.628679 3:-91836870 None:intergenic
AGCATGCACAGAAGGGACCA+TGG 0.630235 3:-91839107 None:intergenic
TATTCTTCAATAGAAACTCA+AGG 0.630493 3:+91835964 MsaT015867.1:CDS
GGCAGTTGGAATCTTGGACA+AGG 0.634216 3:+91838931 MsaT015867.1:CDS
TCATGCCACAACTGAGAATG+GGG 0.637586 3:-91837436 None:intergenic
GCGGCCAGCAATGGGCACAA+GGG 0.639651 3:+91838214 MsaT015867.1:CDS
GGATGGCGTAATCTGTTGTG+AGG 0.646267 3:+91836932 MsaT015867.1:CDS
CGAGTGATGAAATGGAAACA+CGG 0.646958 3:+91839555 MsaT015867.1:CDS
CTGCTACTATGCACATGGAG+AGG 0.651839 3:+91830174 MsaT015867.1:CDS
AGGAGAACCGTAGTTTCCAG+AGG 0.651888 3:+91830194 MsaT015867.1:CDS
AGCTGCTGAAGGATAAGCTG+CGG 0.660445 3:+91837297 MsaT015867.1:CDS
GATGACGAGCATGCACAGAA+GGG 0.661662 3:-91839114 None:intergenic
ATTCATGATGACTATATCCG+AGG 0.671055 3:-91831417 None:intergenic
AAGCTTGAATATGCATCCCG+AGG 0.674899 3:-91836039 None:intergenic
GAGGTGACCTCTAAGAACCC+AGG 0.676062 3:-91831398 None:intergenic
CCTGCTGAAATAATTCAGGA+TGG 0.681187 3:+91836915 MsaT015867.1:CDS
CCTTTATAATCGAAGCAGAG+AGG 0.688981 3:-91837142 None:intergenic
GCATTACAGAGAAACAAATG+AGG 0.696255 3:+91830598 MsaT015867.1:CDS
AGGTGGCGGAGAAAACGGGT+TGG 0.696971 3:+91838962 MsaT015867.1:CDS
TGGGTTCTTAGAGGTCACCT+CGG 0.705452 3:+91831400 MsaT015867.1:CDS
GATGATAGCTCAGAATCTGG+AGG 0.706195 3:+91837164 MsaT015867.1:CDS
ATTGGCATAACAATATTCTG+GGG 0.709888 3:-91836103 None:intergenic
TGAGGGGTTACCAGACTCGG+AGG 0.712583 3:+91838888 MsaT015867.1:CDS
AAGCCCAGGTAGAATCCGAG+GGG 0.750615 3:-91836869 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 91828369 91839670 91828369 ID=MsaG015867
Chr3 mRNA 91828369 91839670 91828369 ID=MsaT015867.1;Parent=MsaG015867
Chr3 exon 91828369 91828378 91828369 ID=MsaT015867.1.exon1;Parent=MsaT015867.1
Chr3 CDS 91828369 91828378 91828369 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91829163 91829294 91829163 ID=MsaT015867.1.exon2;Parent=MsaT015867.1
Chr3 CDS 91829163 91829294 91829163 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91829413 91829528 91829413 ID=MsaT015867.1.exon3;Parent=MsaT015867.1
Chr3 CDS 91829413 91829528 91829413 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91830151 91830239 91830151 ID=MsaT015867.1.exon4;Parent=MsaT015867.1
Chr3 CDS 91830151 91830239 91830151 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91830574 91830619 91830574 ID=MsaT015867.1.exon5;Parent=MsaT015867.1
Chr3 CDS 91830574 91830619 91830574 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91831266 91831623 91831266 ID=MsaT015867.1.exon6;Parent=MsaT015867.1
Chr3 CDS 91831266 91831623 91831266 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91835187 91835338 91835187 ID=MsaT015867.1.exon7;Parent=MsaT015867.1
Chr3 CDS 91835187 91835338 91835187 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91835732 91835822 91835732 ID=MsaT015867.1.exon8;Parent=MsaT015867.1
Chr3 CDS 91835732 91835822 91835732 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91835917 91836137 91835917 ID=MsaT015867.1.exon9;Parent=MsaT015867.1
Chr3 CDS 91835917 91836137 91835917 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91836840 91837519 91836840 ID=MsaT015867.1.exon10;Parent=MsaT015867.1
Chr3 CDS 91836840 91837519 91836840 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91838114 91838412 91838114 ID=MsaT015867.1.exon11;Parent=MsaT015867.1
Chr3 CDS 91838114 91838412 91838114 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91838481 91838671 91838481 ID=MsaT015867.1.exon12;Parent=MsaT015867.1
Chr3 CDS 91838481 91838671 91838481 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91838881 91839174 91838881 ID=MsaT015867.1.exon13;Parent=MsaT015867.1
Chr3 CDS 91838881 91839174 91838881 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Chr3 exon 91839539 91839670 91839539 ID=MsaT015867.1.exon14;Parent=MsaT015867.1
Chr3 CDS 91839539 91839670 91839539 ID=cds.MsaT015867.1;Parent=MsaT015867.1
Gene Sequence

>MsaT015867.1

ATGCTACCTGGTTTGCAATACAACATCAACGATCTGTTTCAAGAAGCTAAGAAAAGATGGCTCAAGCCTATAGAAGTGCTTTACATTTTGCAGAATCATGATACCTGCAAGTTCACCGATTTTCCCCTTAACCAGCCTCATGGTGGATCTTTGTATCTGTTTAATAGAAGAGTCATGCGTTTCTTTCGAAAAGATGGTCATAATTGGCGGAAGAAAAAAGATGGAAGAACTGTTTCAGAAGCGCATGAACGGCTTAAGGTTGGAAATGTTGAAGCTCTAAACTGCTACTATGCACATGGAGAGGAGAACCGTAGTTTCCAGAGGCGAAGTTATTGGATGTTGAACCCGGAATATGAGCATGTTGTTCTCGTGCATTACAGAGAAACAAATGAGGGGACATCCAATTCTGGTCCTGTCACACAATCATCACCGTTTAGTCAGAGTCGTAGCTCATATACTACTCCAAACCCTGAGACAACATCCATAGTTGGTGATTCATGTGAACCTAATCAGAATTTCTCCAGTCCTGGGTTCTTAGAGGTCACCTCGGATATAGTCATCATGAATAATGAAACGGATCACTTAGAGAAAACAAACGCACAAGCTCTGCGCCAATTGGAGGAACAATTAAGTTTGAATGAGGACAGCTTCACAGAAATTCCACCCTTTTATAATGAGCATGAAATTCCTGTTGCATTCGCTGAACCAGATGATCATAAACAACCATATGATGGATATAATGGAACAAAAGATTGCAGTGGTAATCGTTATCATGAATTACTTGACCATGATTGCCCTGCTGGGCATGAAAAAACCTTATCTTGGACAGAGATGCTGGAATCATCCAAGTCCTCATTTGCAAACAAGTTACCAAAACAACACGCATACAAGGAATTTGAAAATGAAACGCCTCTATCTTCTTTTGGAAGAGAAATGATTGCCAACCAGGAAACCAGTTACCGGATACACCCCAACAGCAATAATGATGAGAACTCATACTTTTTACTTCCTCAAGACACTGGTGGAGTCCAATTTTCTCCATATTCTTCAATAGAAACTCAAGGAACTAATTCTGACTACTATGAAACATTATTTGACCAAAGCCAAATTCAAGAACCTCGGGATGCATATTCAAGCTTGACTGTTGGCCAGAAACAGAAATTTACAATTACAGCTGTCTCCCCAGAATATTGTTATGCCAATGAGGCAACAAAGGTGATCATTGTTGGATCCTTTCTCTGCCTCCCCTCGGATTCTACCTGGGCTTGTATGTTTGGTGACGTTGAAGTTCCTGCTGAAATAATTCAGGATGGCGTAATCTGTTGTGAGGCTCCGTCCCATCTTCTTGGAAAGGTTGCTCTGTGCATTACTTCAGGAAATAAGGAACCATGCAGTGAAATCAAAGAGTTTGAGTTTCGTAATAAGACCAATAGTTGCATTCACTGTAATGTTTTGGAAACAGAAGTTGCCCATAGTCCAGAAGAACTGTTATTACTTGTTCGATTTGCAGAAATGCTCCTCTCTGCTTCGATTATAAAGGATGATAGCTCAGAATCTGGAGGTCAATTTTCAACAGAACAGAAAGCAGATGATGATTCATGGAGCCATATTATAGATGCTCTCCTAGTTGGCAACGTAAATTCATCTGGTACTATTGATTGTCTTCTTCAAGAGCTGCTGAAGGATAAGCTGCGGCACTGGCTTTCTTGCAGATCCAATGAACGAGATGAAGATGCAGGTTGTTCTTTGTCCAAGAAAGAGCAAGGGATTATACACATAGTTTCTGGATTGGGTTTCGAGTGGGCCCTGAACCCCATTCTCAGTTGTGGCATGAATGTTAATTTCCGTGATATCAATGGATGGACTGCCCTTCATTGGGCTGCACGGTTTGGAAGGGAAAAAATGGTTGCTTCACTTATTGCTGCTGGTGCATCTGCTGGAGCAGTGACGGATCCAAGTTCACAAGATCCAAATGGTAAAACTGCTGCATCTATTGCGGCCAGCAATGGGCACAAGGGACTGGCAGGTTATCTTGCAGAAGTAGATCTAACAAGTCATCTGTCATCCCTCACATTGGAAAAGTGTGAAGTTCCTAAAGATTCTTCTGAGCTTGAAGCTGAGTTAACTGTCAGTAGTGTGTCTAAGAAAAATCTTGAAGCCAGTGATGATGAGGATTCACTAAGAAATACCTTGGAAAACGGATGGAGAGAAGCTGCCTCCACCACTTGTCTAAATGGATATGTCACCGGTTTAGGTGGCATTGGTGGATATGTTAGGAGCTCACGTGATTACAATTCAGCTGCCTTATCAATTCAGAAGAAATATAGAGGCTGGAAAGTTCGCAAAGAGTACTTAGCTTTTCGCCAAAAAGTTGTAACGATACAGGCTCATGTGAGGGGTTACCAGACTCGGAGGCAATACAAGCTAATGATATGGGCAGTTGGAATCTTGGACAAGGTTGTGCTAAGGTGGCGGAGAAAACGGGTTGGTTTGCGAAGTTCTCCACAAGAAATAGATTCAAAAGAAGAAAGTGATGATGAAGATTTTCTCAAGGTGTTCAGGCAAGAAAAAGTACATGCAGCAATTCAAAAGGCTTTGGCACGGGTGATTTCCATGGTCCCTTCTGTGCATGCTCGTCATCAGTATAATCGCATGCTTGGGATGCGTCGTCGAGCCGAGGCTGAACATGGCAACACGAGTGATGAAATGGAAACACGGTTATCAACTTCTGTGGATGATGCTTGGAATATTGATGATGCTTGGAATGTTGAAGATGATGATTTATATCAATTTCCTTGGGAAGCGCTCTAG
Protein sequence

>MsaT015867.1

MLPGLQYNINDLFQEAKKRWLKPIEVLYILQNHDTCKFTDFPLNQPHGGSLYLFNRRVMRFFRKDGHNWRKKKDGRTVSEAHERLKVGNVEALNCYYAHGEENRSFQRRSYWMLNPEYEHVVLVHYRETNEGTSNSGPVTQSSPFSQSRSSYTTPNPETTSIVGDSCEPNQNFSSPGFLEVTSDIVIMNNETDHLEKTNAQALRQLEEQLSLNEDSFTEIPPFYNEHEIPVAFAEPDDHKQPYDGYNGTKDCSGNRYHELLDHDCPAGHEKTLSWTEMLESSKSSFANKLPKQHAYKEFENETPLSSFGREMIANQETSYRIHPNSNNDENSYFLLPQDTGGVQFSPYSSIETQGTNSDYYETLFDQSQIQEPRDAYSSLTVGQKQKFTITAVSPEYCYANEATKVIIVGSFLCLPSDSTWACMFGDVEVPAEIIQDGVICCEAPSHLLGKVALCITSGNKEPCSEIKEFEFRNKTNSCIHCNVLETEVAHSPEELLLLVRFAEMLLSASIIKDDSSESGGQFSTEQKADDDSWSHIIDALLVGNVNSSGTIDCLLQELLKDKLRHWLSCRSNERDEDAGCSLSKKEQGIIHIVSGLGFEWALNPILSCGMNVNFRDINGWTALHWAARFGREKMVASLIAAGASAGAVTDPSSQDPNGKTAASIAASNGHKGLAGYLAEVDLTSHLSSLTLEKCEVPKDSSELEAELTVSSVSKKNLEASDDEDSLRNTLENGWREAASTTCLNGYVTGLGGIGGYVRSSRDYNSAALSIQKKYRGWKVRKEYLAFRQKVVTIQAHVRGYQTRRQYKLMIWAVGILDKVVLRWRRKRVGLRSSPQEIDSKEESDDEDFLKVFRQEKVHAAIQKALARVISMVPSVHARHQYNRMLGMRRRAEAEHGNTSDEMETRLSTSVDDAWNIDDAWNVEDDDLYQFPWEAL*