AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG015870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015870 MtrunA17_Chr3g0122391 95.363 992 23 1 1 969 36 1027 0.0 1957
MsaG015870 MtrunA17_Chr8g0377481 77.755 980 184 6 14 969 1 970 0.0 1528
MsaG015870 MtrunA17_Chr5g0447641 53.531 977 374 14 47 966 259 1212 0.0 996
MsaG015870 MtrunA17_Chr8g0392301 50.352 995 407 16 38 968 25 996 0.0 928
MsaG015870 MtrunA17_Chr2g0311191 42.268 291 91 3 208 498 21 234 1.40e-61 209
MsaG015870 MtrunA17_Chr1g0150511 33.542 319 149 9 408 666 379 694 1.30e-34 143
MsaG015870 MtrunA17_Chr1g0150511 32.867 143 76 3 843 966 870 1011 3.43e-13 73.9
MsaG015870 MtrunA17_Chr5g0405701 29.101 378 216 8 309 651 404 764 2.93e-31 132
MsaG015870 MtrunA17_Chr5g0405701 34.932 146 64 2 835 954 1044 1184 6.70e-17 86.3
MsaG015870 MtrunA17_Chr3g0136601 34.138 290 159 7 408 667 373 660 1.11e-30 130
MsaG015870 MtrunA17_Chr3g0136601 30.667 150 83 3 838 967 801 949 3.79e-14 77.0
MsaG015870 MtrunA17_Chr8g0371601 31.329 316 176 5 361 651 427 726 1.51e-30 130
MsaG015870 MtrunA17_Chr8g0371601 34.161 161 81 4 814 954 999 1154 4.60e-17 86.7
MsaG015870 MtrunA17_Chr4g0013831 28.412 359 193 7 396 728 335 655 4.80e-29 124
MsaG015870 MtrunA17_Chr4g0013831 33.884 121 72 3 838 958 612 724 7.93e-12 69.3
MsaG015870 MtrunA17_Chr7g0269631 27.365 296 154 6 396 667 335 593 1.95e-24 109
MsaG015870 MtrunA17_Chr1g0197591 27.027 296 168 8 398 659 371 652 1.42e-20 98.2
MsaG015870 MtrunA17_Chr1g0197591 37.313 134 64 4 838 962 923 1045 1.13e-16 85.5
MsaG015870 MtrunA17_Chr1g0194741 26.690 281 149 9 397 636 387 651 9.65e-19 91.7
MsaG015870 MtrunA17_Chr7g0274831 36.220 127 67 4 840 962 638 754 1.64e-15 81.3
MsaG015870 MtrunA17_Chr8g0343551 46.914 81 32 3 882 962 13 82 4.27e-15 71.6
MsaG015870 MtrunA17_Chr1g0194731 33.577 137 68 6 838 963 325 449 1.23e-14 77.8
MsaG015870 MtrunA17_Chr1g0186431 31.298 131 82 3 837 966 631 754 2.49e-14 77.4
MsaG015870 MtrunA17_Chr1g0190021 35.200 125 72 3 840 962 634 751 6.58e-13 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG015870 AT1G27320.1 65.388 991 286 10 14 968 61 1030 0.0 1264
MsaG015870 AT5G35750.1 54.180 969 372 13 47 967 230 1174 0.0 1007
MsaG015870 AT2G01830.1 51.484 977 393 15 44 967 101 1049 0.0 937
MsaG015870 AT2G01830.4 51.484 977 393 15 44 967 101 1049 0.0 937
MsaG015870 AT2G01830.6 51.484 977 393 15 44 967 101 1049 0.0 937
MsaG015870 AT2G01830.3 51.484 977 393 15 44 967 101 1049 0.0 937
MsaG015870 AT2G01830.5 51.484 977 393 15 44 967 113 1061 0.0 937
MsaG015870 AT2G01830.2 51.484 977 393 15 44 967 124 1072 0.0 937
MsaG015870 AT2G17820.1 31.288 326 181 7 361 660 454 762 9.59e-34 141
MsaG015870 AT2G17820.1 32.215 149 64 2 838 954 1041 1184 1.38e-14 79.3
MsaG015870 AT5G10720.1 33.210 271 127 5 408 655 369 608 2.69e-32 136
MsaG015870 AT5G10720.1 31.944 144 78 3 842 966 779 921 6.21e-15 80.1
MsaG015870 AT1G66340.1 28.889 360 186 7 396 728 334 650 2.88e-29 125
MsaG015870 AT2G40940.1 29.927 274 133 4 396 646 334 571 1.65e-25 113
MsaG015870 AT2G47430.1 26.814 317 181 8 393 673 383 684 3.63e-24 110
MsaG015870 AT2G47430.1 36.076 158 78 7 822 962 966 1117 2.64e-15 81.6
MsaG015870 AT3G04580.2 32.298 161 94 4 811 969 617 764 2.27e-15 81.3
MsaG015870 AT3G04580.1 32.298 161 94 4 811 969 617 764 2.27e-15 81.3
MsaG015870 AT3G23150.1 29.412 153 93 2 819 966 624 766 3.82e-12 70.9

Find 226 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATCTATTGATGTGCTTTC+TGG 0.199915 3:-91870074 MsaT015870.1:CDS
ATTGTTGCTGGCATGGGTTT+TGG 0.201381 3:-91870958 MsaT015870.1:CDS
GTTTGTTCATCTACCCATTT+TGG 0.201868 3:-91866253 MsaT015870.1:CDS
TTAACTTTGGAGATCCTTTC+AGG 0.201914 3:-91869218 MsaT015870.1:CDS
GTTCGATATACGAGCAATTA+TGG 0.223643 3:-91868245 MsaT015870.1:CDS
GTCAATTATAAATGAAGTTT+TGG 0.241047 3:-91868314 MsaT015870.1:CDS
CCTGCAACTTCAGAGTTTAA+TGG 0.244003 3:-91867355 MsaT015870.1:CDS
GTTGCTTGAATCCTTTCATT+TGG 0.253731 3:+91869829 None:intergenic
TTGCTTGAATCCTTTCATTT+GGG 0.258270 3:+91869830 None:intergenic
GTTTGAAGCTCATTTGAATT+AGG 0.258603 3:+91867403 None:intergenic
GTCTGCGGTCATAGCCAAAA+TGG 0.274932 3:+91866239 None:intergenic
GTTGGTTTATAGTGTCTCTA+TGG 0.276176 3:-91870935 MsaT015870.1:CDS
TCTCTCAAGCATCTTCTCTC+TGG 0.280566 3:-91866896 MsaT015870.1:CDS
TTAACGAGAATAAATAGTTT+TGG 0.294927 3:+91867079 None:intergenic
TCAATTTGATGCATGTTTCA+TGG 0.295254 3:-91866741 MsaT015870.1:CDS
CTTGTTATTGCACTCCTTGT+TGG 0.299522 3:-91868770 MsaT015870.1:CDS
AATGCATACCCATCCATTTC+AGG 0.300024 3:+91866707 None:intergenic
TCTCATGGGTAACTCGATTA+AGG 0.309745 3:-91867981 MsaT015870.1:intron
CCCAAACCTCTTGTTCAATC+AGG 0.316912 3:+91867182 None:intergenic
ATGCTGATGGTCGTCGCAGC+TGG 0.318940 3:-91867735 MsaT015870.1:CDS
TACACTGATAATACTGTTGA+TGG 0.335179 3:+91871018 None:intergenic
TCAGAACCATACATGGGAAT+TGG 0.339185 3:+91869276 None:intergenic
CGCACTTTGTGAATAACATT+AGG 0.352181 3:-91867138 MsaT015870.1:CDS
GACCATGCAAAGATTGAATC+TGG 0.357737 3:-91868292 MsaT015870.1:CDS
TCCCACACTTAGAGCATTCC+TGG 0.359931 3:+91866199 None:intergenic
CTGCGACGACCATCAGCATC+TGG 0.361711 3:+91867739 None:intergenic
CATGTGAGCTTTCTTAACTT+TGG 0.362535 3:-91869231 MsaT015870.1:CDS
AAAGTTGTTGAAGTTGATAA+AGG 0.366112 3:-91867115 MsaT015870.1:CDS
TCCTCGAAACGGAGAACATC+AGG 0.366537 3:-91866924 MsaT015870.1:CDS
CCCTGATGTTCTCCGTTTCG+AGG 0.366643 3:+91866923 None:intergenic
CCATTAAACTCTGAAGTTGC+AGG 0.374363 3:+91867355 None:intergenic
TTTGTGAGCGAACCGAAGAT+TGG 0.374889 3:-91867466 MsaT015870.1:CDS
TCCTGATTGAACAAGAGGTT+TGG 0.378231 3:-91867183 MsaT015870.1:CDS
GGATATTCAAATGCCTGAAA+TGG 0.382771 3:-91866720 MsaT015870.1:CDS
CAGGTTCCTCACCAGCTTAT+AGG 0.385173 3:-91868037 MsaT015870.1:CDS
TGATTATGTACCAGATGAAT+TGG 0.385215 3:-91870162 MsaT015870.1:CDS
ATTATTTGTCGAAACCTTCC+AGG 0.393734 3:+91868010 None:intergenic
ACATAATCATCCTCGTGAAC+CGG 0.396359 3:+91870176 None:intergenic
GATGCAGAACATACATTAAG+TGG 0.399490 3:-91867766 MsaT015870.1:CDS
TGACAGGAGCATATTCTTCA+TGG 0.406008 3:+91870126 None:intergenic
TCATTTGGGGTAGCATTTGA+TGG 0.407780 3:+91869844 None:intergenic
CAACAGTATTATCAGTGTAT+TGG 0.408995 3:-91871014 MsaT015870.1:CDS
TGAAACATGCATCAAATTGA+TGG 0.409814 3:+91866744 None:intergenic
TAACCTAAAACACCGTTCAT+TGG 0.415965 3:+91868513 None:intergenic
CCTGATTGAACAAGAGGTTT+GGG 0.417274 3:-91867182 MsaT015870.1:CDS
GTGTTGCGAGCAAGAGAATC+AGG 0.418715 3:-91869955 MsaT015870.1:CDS
GTGAAAGTGCTTCCAATCTT+CGG 0.418916 3:+91867454 None:intergenic
CTCGACACTTCAGCACGAAC+GGG 0.429876 3:+91867304 None:intergenic
GTATTGGTTCTAAAAGAGTT+AGG 0.431830 3:-91870998 MsaT015870.1:CDS
AGCAATTTATGTCTCGGATC+AGG 0.436945 3:-91868056 MsaT015870.1:CDS
TCTGAGAAATCTCAAGGAAA+AGG 0.440712 3:-91868202 MsaT015870.1:CDS
AATCAAACTGATGAGATCAT+TGG 0.444384 3:+91867654 None:intergenic
CTCTCAAGCATCTTCTCTCT+GGG 0.446499 3:-91866895 MsaT015870.1:CDS
AGATGCATCCCCATCGGTTA+CGG 0.446690 3:+91867216 None:intergenic
GGCCTCATGAATGGAGAAAT+CGG 0.447591 3:-91867490 MsaT015870.1:CDS
CCCGTCGCCAACCTTGGAAA+TGG 0.448066 3:+91868809 None:intergenic
ATTGTATCGGTAGAGGATAC+AGG 0.449292 3:-91867634 MsaT015870.1:CDS
TACAAAGAGCAATGGGTGTT+GGG 0.450440 3:-91866958 MsaT015870.1:CDS
TAAATGTTGGGAGAAGATCT+TGG 0.452217 3:-91871057 MsaT015870.1:CDS
TCGAGAAAACACGGAGGAAC+TGG 0.453005 3:-91867544 MsaT015870.1:CDS
CAGAACCATACATGGGAATT+GGG 0.453538 3:+91869277 None:intergenic
TTGGCGACGGGTACATCAAT+TGG 0.453701 3:-91868797 MsaT015870.1:CDS
TAAAGTTGTTCAGCTTCAAA+TGG 0.453871 3:+91866156 None:intergenic
AAATGTTGGGAGAAGATCTT+GGG 0.457357 3:-91871056 MsaT015870.1:CDS
TTCAGTCACGAGGGACCTCT+TGG 0.458368 3:-91867697 MsaT015870.1:CDS
TTGGTAATCATCCTCTACTT+TGG 0.458958 3:+91868715 None:intergenic
TGGGGATGCATCTATCAATA+TGG 0.459409 3:-91867206 MsaT015870.1:CDS
ACAAATAATTACAAATCTCA+TGG 0.460319 3:-91867996 MsaT015870.1:CDS
ATGTATGGTTCTGATGTATC+AGG 0.460892 3:-91869267 MsaT015870.1:CDS
AACTCTGAAGTTGCAGGATG+AGG 0.466527 3:+91867361 None:intergenic
TGCTATGCAGGTATCTTGGT+GGG 0.467017 3:-91869398 MsaT015870.1:intron
CACCAGCTTATAGGTGATCC+TGG 0.468817 3:-91868028 MsaT015870.1:CDS
TAACTGCTATGCAGGTATCT+TGG 0.469122 3:-91869402 MsaT015870.1:intron
TGTTTAGTGGGCCTCATGAA+TGG 0.472002 3:-91867499 MsaT015870.1:CDS
CTGTTGAAAACAAACCGTTT+AGG 0.473410 3:-91869904 MsaT015870.1:CDS
GGGGATGGTTCCAATTCATC+TGG 0.475795 3:+91870152 None:intergenic
AACTTCAGAGTTTAATGGTA+TGG 0.482042 3:-91867350 MsaT015870.1:CDS
GGAGGAAATTGTTGCTGGCA+TGG 0.482367 3:-91870965 MsaT015870.1:CDS
CATTAGTATGGACACAAAGA+TGG 0.482671 3:-91871111 None:intergenic
ATTGTGGAGGAAATTGTTGC+TGG 0.485555 3:-91870970 MsaT015870.1:CDS
AAAGTTAGCAATTTATGTCT+CGG 0.486930 3:-91868062 MsaT015870.1:intron
GACTATCCATCTTGTTGAGG+AGG 0.486988 3:-91867830 MsaT015870.1:CDS
AAGCATTAGCAAGTGTTTAG+TGG 0.490010 3:-91867512 MsaT015870.1:CDS
GATGGACCTACCTGCATGAA+TGG 0.490106 3:+91867571 None:intergenic
GAGCATATTCTTCATGGATA+GGG 0.491204 3:+91870132 None:intergenic
AACAAGGGTGGAGTATTAAG+AGG 0.492013 3:-91870220 MsaT015870.1:CDS
TATTGATGTGCTTTCTGGAA+AGG 0.494599 3:-91870069 MsaT015870.1:intron
GTTTGAAATAGAGTCATTAG+TGG 0.495045 3:-91869373 MsaT015870.1:CDS
TGTGACTATCCATCTTGTTG+AGG 0.496121 3:-91867833 MsaT015870.1:CDS
ATGTGAGGATAACTCCTAAA+CGG 0.497426 3:+91869890 None:intergenic
TGCTCTAAGTGTGGGATGGA+TGG 0.499675 3:-91866192 MsaT015870.1:CDS
GATGTACCCGTCGCCAACCT+TGG 0.499801 3:+91868803 None:intergenic
CAGTTTGATTGTATCGGTAG+AGG 0.505448 3:-91867641 MsaT015870.1:CDS
GAAAGATGTGGCTTAAATGT+TGG 0.505804 3:-91871070 MsaT015870.1:CDS
CAATGGGTGTTGGGAACAAA+GGG 0.506109 3:-91866949 MsaT015870.1:CDS
GAAACCACCATTTCCAAGGT+TGG 0.506338 3:-91868816 MsaT015870.1:CDS
CTGCTATGCAGGTATCTTGG+TGG 0.507522 3:-91869399 MsaT015870.1:intron
ACACACCCAATTCCCATGTA+TGG 0.507762 3:-91869282 MsaT015870.1:CDS
GAGGAAATTGTTGCTGGCAT+GGG 0.509124 3:-91870964 MsaT015870.1:CDS
AAACACGGAGGAACTGGTAT+TGG 0.510837 3:-91867538 MsaT015870.1:CDS
ATTTGAATTAGGACATGCAT+TGG 0.511417 3:+91867414 None:intergenic
GGAGCATATTCTTCATGGAT+AGG 0.512983 3:+91870131 None:intergenic
GTGGTGTTGCATATGCTGTA+AGG 0.513848 3:-91870277 MsaT015870.1:CDS
AGCTGATGTTGCTAAATCCC+AGG 0.514509 3:-91868660 MsaT015870.1:intron
CTACAAAGAGCAATGGGTGT+TGG 0.514952 3:-91866959 MsaT015870.1:CDS
ACCATTTCCAAGGTTGGCGA+CGG 0.515570 3:-91868810 MsaT015870.1:CDS
ATAGTTTCAGATTTGAAGCA+AGG 0.519061 3:-91867247 MsaT015870.1:CDS
CATAATCATCCTCGTGAACC+GGG 0.519384 3:+91870177 None:intergenic
TGATACATCAGAACCATACA+TGG 0.519775 3:+91869269 None:intergenic
ATTCAAATGCCTGAAATGGA+TGG 0.520727 3:-91866716 MsaT015870.1:CDS
CGTGATGCAGGCTACACACC+AGG 0.521945 3:-91866217 MsaT015870.1:CDS
AACAGAAACCACCATTTCCA+AGG 0.522429 3:-91868820 MsaT015870.1:CDS
TGCTGATGGTCGTCGCAGCT+GGG 0.525255 3:-91867734 MsaT015870.1:CDS
ACTTCAGCACGAACGGGTCT+CGG 0.525496 3:+91867310 None:intergenic
GAGCAAGAGAATCAGGCAAA+GGG 0.525841 3:-91869948 MsaT015870.1:CDS
ATAAGGCAGTGGAAAAGCTC+TGG 0.526856 3:-91868338 MsaT015870.1:intron
GTGAATCGCACGGTAGCAGC+TGG 0.527329 3:-91866842 MsaT015870.1:CDS
TCTGCGGTCATAGCCAAAAT+GGG 0.530084 3:+91866240 None:intergenic
GGAAGCATGAGATGCACTGC+AGG 0.532148 3:-91869197 MsaT015870.1:intron
AAGATTAGGTACCTGGTCAA+TGG 0.533997 3:+91870743 None:intergenic
GTGAACCGGGTTCTGCTCCA+TGG 0.534066 3:+91870190 None:intergenic
TATGGTCCTGATTGAACAAG+AGG 0.534402 3:-91867188 MsaT015870.1:CDS
AGCTTATAGGTGATCCTGGA+AGG 0.535437 3:-91868024 MsaT015870.1:CDS
GCTTCACTACAAAGAGCAAT+GGG 0.535937 3:-91866965 MsaT015870.1:CDS
AAAGATGTGGCTTAAATGTT+GGG 0.536867 3:-91871069 MsaT015870.1:CDS
GATGGTCGTCGCAGCTGGGA+AGG 0.537082 3:-91867730 MsaT015870.1:CDS
TGCTTGAATCCTTTCATTTG+GGG 0.537514 3:+91869831 None:intergenic
TCTCATCAGTTTGATTGTAT+CGG 0.538336 3:-91867647 MsaT015870.1:CDS
TATGACCGCAGACGTGATGC+AGG 0.539120 3:-91866229 MsaT015870.1:CDS
GGAAAACCTCCTCAACAAGA+TGG 0.540597 3:+91867824 None:intergenic
ACTTACACAACACTGAAAAG+AGG 0.541604 3:-91870896 MsaT015870.1:CDS
CAGAAGGATTCTTAGCATGA+TGG 0.541718 3:+91870766 None:intergenic
TAGGAGATGGTGGAAAGATG+TGG 0.542063 3:-91871082 MsaT015870.1:CDS
TACCAGATTCAATCTTTGCA+TGG 0.542356 3:+91868290 None:intergenic
CTGAAGTTGCAGGATGAGGT+TGG 0.542365 3:+91867365 None:intergenic
AAAGAGTTAGGAGAAAATTG+TGG 0.543247 3:-91870986 MsaT015870.1:CDS
GAAAGGATTCAAGCAACTGA+TGG 0.545412 3:-91869823 MsaT015870.1:CDS
TAAGGGTGCTTCAATCTGAA+AGG 0.545666 3:-91870259 MsaT015870.1:CDS
GGATGATTACCAAGAGATGA+TGG 0.546326 3:-91868705 MsaT015870.1:CDS
AAGGGTGCTTCAATCTGAAA+GGG 0.547300 3:-91870258 MsaT015870.1:CDS
AGGGTGGAGTATTAAGAGGA+TGG 0.548363 3:-91870216 MsaT015870.1:CDS
GTATCTTGTGCAAAGATGAC+AGG 0.548377 3:+91870110 None:intergenic
TATGCAACACCACTTGTAAG+TGG 0.549161 3:+91870287 None:intergenic
ATTAGTATGGACACAAAGAT+GGG 0.549576 3:-91871110 None:intergenic
TTCCAGGATCACCTATAAGC+TGG 0.549721 3:+91868026 None:intergenic
GCAATGGGTGTTGGGAACAA+AGG 0.550130 3:-91866950 MsaT015870.1:CDS
AGCATTAGCAAGTGTTTAGT+GGG 0.550350 3:-91867511 MsaT015870.1:CDS
GAAACATGCATCAAATTGAT+GGG 0.550648 3:+91866745 None:intergenic
GATGAGATCATTGGAGGAGG+TGG 0.554361 3:+91867663 None:intergenic
CCATTTCCAAGGTTGGCGAC+GGG 0.554660 3:-91868809 MsaT015870.1:CDS
TGCATCTCATGCTTCCTGAA+AGG 0.558589 3:+91869204 None:intergenic
AACAAAGGGAATCCTCGAAA+CGG 0.558711 3:-91866935 MsaT015870.1:CDS
GTTTGTCCACCGTAACCGAT+GGG 0.559704 3:-91867225 MsaT015870.1:CDS
GGGTGGCCTCAGCATAGTGA+TGG 0.560543 3:+91866766 None:intergenic
TTCAAATGCCTGAAATGGAT+GGG 0.561638 3:-91866715 MsaT015870.1:intron
GGTTTGTCCACCGTAACCGA+TGG 0.562427 3:-91867226 MsaT015870.1:CDS
GGCTTCACTACAAAGAGCAA+TGG 0.563329 3:-91866966 MsaT015870.1:CDS
ATCATACACATTAACAAGCA+CGG 0.567202 3:+91869317 None:intergenic
AGATATCACATACAAAGGCT+TGG 0.567391 3:-91867283 MsaT015870.1:CDS
GAATCCTTCTGCCATTGACC+AGG 0.567998 3:-91870754 MsaT015870.1:intron
CAAATAATTACAAATCTCAT+GGG 0.572811 3:-91867995 MsaT015870.1:CDS
TATTCTTCATGGATAGGGGA+TGG 0.574785 3:+91870137 None:intergenic
TCTCGACACTTCAGCACGAA+CGG 0.575293 3:+91867303 None:intergenic
GCAAGCATACTTGCTCTCAA+AGG 0.577203 3:+91866989 None:intergenic
AAAGGATTCAAGCAACTGAT+GGG 0.577305 3:-91869822 MsaT015870.1:intron
GATATTCACTCCATTCATGC+AGG 0.578156 3:-91867581 MsaT015870.1:CDS
AAGAGGTCCCTCGTGACTGA+AGG 0.581373 3:+91867699 None:intergenic
AGCTGAACAACTTTATAGAG+AGG 0.581697 3:-91866148 MsaT015870.1:CDS
CAAATGCTACCCCAAATGAA+AGG 0.586250 3:-91869840 MsaT015870.1:CDS
AGTGGTTTCCCAGATGCTGA+TGG 0.586487 3:-91867748 MsaT015870.1:CDS
AACAATTTGAGAAGCAACAA+GGG 0.589239 3:-91870235 MsaT015870.1:CDS
AGCATATTCTTCATGGATAG+GGG 0.589325 3:+91870133 None:intergenic
CACCAGGAATGCTCTAAGTG+TGG 0.589718 3:-91866201 MsaT015870.1:CDS
CAAACTGATGAGATCATTGG+AGG 0.589840 3:+91867657 None:intergenic
CGAGCAAGAGAATCAGGCAA+AGG 0.590048 3:-91869949 MsaT015870.1:CDS
CATGCATCAAATTGATGGGG+TGG 0.590211 3:+91866749 None:intergenic
CCATCCATCTCGAGAAAACA+CGG 0.590517 3:-91867553 MsaT015870.1:CDS
CAACAAGGAGTGCAATAACA+AGG 0.591437 3:+91868771 None:intergenic
GTTTGGCAGTTCACAGACAA+AGG 0.593004 3:-91867865 MsaT015870.1:intron
TTTGAGAGCAAGTATGCTTG+CGG 0.593125 3:-91866987 MsaT015870.1:CDS
TGCGACGACCATCAGCATCT+GGG 0.593941 3:+91867740 None:intergenic
GGATACCATGGAGCAGAACC+CGG 0.595639 3:-91870195 MsaT015870.1:CDS
GTCGCCAACCTTGGAAATGG+TGG 0.598690 3:+91868812 None:intergenic
GAACAATTTGAGAAGCAACA+AGG 0.603982 3:-91870236 MsaT015870.1:CDS
TGTAGCCTGCATCACGTCTG+CGG 0.606500 3:+91866224 None:intergenic
GCTATGCAGGTATCTTGGTG+GGG 0.608136 3:-91869397 MsaT015870.1:intron
ATCCGATTTCTCCATTCATG+AGG 0.608906 3:+91867488 None:intergenic
TATTAAGAGGATGGATACCA+TGG 0.611537 3:-91870207 MsaT015870.1:CDS
TGGTGTTGCATATGCTGTAA+GGG 0.613148 3:-91870276 MsaT015870.1:CDS
ATCTCAAGGAAAAGGAGCTG+AGG 0.613896 3:-91868194 MsaT015870.1:intron
TGCATCCCCATCGGTTACGG+TGG 0.616687 3:+91867219 None:intergenic
ACCAGGAATGCTCTAAGTGT+GGG 0.617004 3:-91866200 MsaT015870.1:CDS
GGAGCAGAACCCGGTTCACG+AGG 0.618481 3:-91870186 MsaT015870.1:CDS
TTTGAGAGGCCACTTACAAG+TGG 0.618617 3:-91870296 MsaT015870.1:CDS
ACTGATGAGATCATTGGAGG+AGG 0.619983 3:+91867660 None:intergenic
GGATCACCTATAAGCTGGTG+AGG 0.621748 3:+91868031 None:intergenic
GAGTCGAATTGCCAAAGTAG+AGG 0.627075 3:-91868726 MsaT015870.1:CDS
TGTCGAGATATCACATACAA+AGG 0.627297 3:-91867288 MsaT015870.1:CDS
ATTGATAGATGCATCCCCAT+CGG 0.631985 3:+91867210 None:intergenic
GCATGTGTGATGAAAGAGCT+AGG 0.632094 3:-91870857 MsaT015870.1:CDS
GGTACCTGGTCAATGGCAGA+AGG 0.637480 3:+91870750 None:intergenic
TGATAATAGTGTGAATCGCA+CGG 0.638695 3:-91866852 MsaT015870.1:CDS
CATGACAAATATGTCCAACA+AGG 0.639906 3:+91868756 None:intergenic
GAGTTAGGAGAAAATTGTGG+AGG 0.641276 3:-91870983 MsaT015870.1:CDS
TCTTGTAAACAGCAAATGTG+AGG 0.641788 3:+91869875 None:intergenic
ATCATCGGCTGCGACTCTAG+AGG 0.643962 3:+91867601 None:intergenic
ATTCAGAGCCTTCAGTCACG+AGG 0.647455 3:-91867707 MsaT015870.1:CDS
ATCTGGTAAGATAGAGCTCG+AGG 0.648865 3:-91868275 MsaT015870.1:CDS
GGAATGCTCTAAGTGTGGGA+TGG 0.650848 3:-91866196 MsaT015870.1:CDS
CCTCGAAACGGAGAACATCA+GGG 0.651041 3:-91866923 MsaT015870.1:CDS
TCGGTAGAGGATACAGGCGA+TGG 0.653480 3:-91867628 MsaT015870.1:CDS
AGTTGAGATCAGAATAGACA+TGG 0.657095 3:+91870791 None:intergenic
ATGAATGGAGTGAATATCAT+CGG 0.658951 3:+91867586 None:intergenic
TTCAGAGCCTTCAGTCACGA+GGG 0.661654 3:-91867706 MsaT015870.1:CDS
AATTTGAGAAGCAACAAGGG+TGG 0.662837 3:-91870232 MsaT015870.1:CDS
GAGATCAGAACTCCAATGAA+CGG 0.671093 3:-91868525 MsaT015870.1:CDS
TAGAAGCCATCACTATGCTG+AGG 0.678727 3:-91866772 MsaT015870.1:CDS
AGCAAGAGAATCAGGCAAAG+GGG 0.689158 3:-91869947 MsaT015870.1:CDS
TTTGTCCACCGTAACCGATG+GGG 0.692151 3:-91867224 MsaT015870.1:CDS
GATACATCAGAACCATACAT+GGG 0.696609 3:+91869270 None:intergenic
TCTCGTGCGAAACAGTAGCA+AGG 0.699858 3:+91868544 None:intergenic
GGTGTGGTTTGTGTAAGCAG+TGG 0.702118 3:-91866797 MsaT015870.1:CDS
AAACATGCATCAAATTGATG+GGG 0.720884 3:+91866746 None:intergenic
TTCACTCCATTCATGCAGGT+AGG 0.724284 3:-91867577 MsaT015870.1:CDS
GTGGACGAGAAAGAACCAAG+AGG 0.763430 3:+91867682 None:intergenic
TCCATCTCGAGAAAACACGG+AGG 0.824559 3:-91867550 MsaT015870.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 91866122 91871126 91866122 ID=MsaG015870
Chr3 mRNA 91866122 91871126 91866122 ID=MsaT015870.1;Parent=MsaG015870
Chr3 exon 91866122 91866332 91866122 ID=MsaT015870.1.exon10;Parent=MsaT015870.1
Chr3 CDS 91866122 91866332 91866122 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Chr3 exon 91866716 91867878 91866716 ID=MsaT015870.1.exon9;Parent=MsaT015870.1
Chr3 CDS 91866716 91867878 91866716 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Chr3 exon 91867982 91868080 91867982 ID=MsaT015870.1.exon8;Parent=MsaT015870.1
Chr3 CDS 91867982 91868080 91867982 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Chr3 exon 91868195 91868355 91868195 ID=MsaT015870.1.exon7;Parent=MsaT015870.1
Chr3 CDS 91868195 91868355 91868195 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Chr3 exon 91868517 91868568 91868517 ID=MsaT015870.1.exon6;Parent=MsaT015870.1
Chr3 CDS 91868517 91868568 91868517 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Chr3 exon 91868661 91868847 91868661 ID=MsaT015870.1.exon5;Parent=MsaT015870.1
Chr3 CDS 91868661 91868847 91868661 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Chr3 exon 91869198 91869410 91869198 ID=MsaT015870.1.exon4;Parent=MsaT015870.1
Chr3 CDS 91869198 91869410 91869198 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Chr3 exon 91869823 91869992 91869823 ID=MsaT015870.1.exon3;Parent=MsaT015870.1
Chr3 CDS 91869823 91869992 91869823 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Chr3 exon 91870070 91870351 91870070 ID=MsaT015870.1.exon2;Parent=MsaT015870.1
Chr3 CDS 91870070 91870351 91870070 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Chr3 exon 91870755 91871126 91870755 ID=MsaT015870.1.exon1;Parent=MsaT015870.1
Chr3 CDS 91870755 91871126 91870755 ID=cds.MsaT015870.1;Parent=MsaT015870.1
Gene Sequence

>MsaT015870.1

ATGGACACAAAGATGGGATTTTTAGGAGATGGTGGAAAGATGTGGCTTAAATGTTGGGAGAAGATCTTGGGAAGTACATGTAAAATCCATCAACAGTATTATCAGTGTATTGGTTCTAAAAGAGTTAGGAGAAAATTGTGGAGGAAATTGTTGCTGGCATGGGTTTTGGGTTGGTTTATAGTGTCTCTATGGATCTTTTGTGGTATTAACTTACACAACACTGAAAAGAGGAAAGAGACTTTAGCTAGCATGTGTGATGAAAGAGCTAGGATGTTACAAGATCAGTTTAATGTTAGTATGAATCATATTCAAGCCATGTCTATTCTGATCTCAACTTTCCATCATGCTAAGAATCCTTCTGCCATTGACCAGAAAACTTTTGCAAAGTATACAGAAAGAACTGCTTTTGAGAGGCCACTTACAAGTGGTGTTGCATATGCTGTAAGGGTGCTTCAATCTGAAAGGGAACAATTTGAGAAGCAACAAGGGTGGAGTATTAAGAGGATGGATACCATGGAGCAGAACCCGGTTCACGAGGATGATTATGTACCAGATGAATTGGAACCATCCCCTATCCATGAAGAATATGCTCCTGTCATCTTTGCACAAGATACAATTTCACATGTCATATCTATTGATGTGCTTTCTGGAAAGGAAGACCGTGAAAATGTGTTGCGAGCAAGAGAATCAGGCAAAGGGGTTTTAACTGCCCCTTTTAGGCTGTTGAAAACAAACCGTTTAGGAGTTATCCTCACATTTGCTGTTTACAAGAGAGATCTTCCATCAAATGCTACCCCAAATGAAAGGATTCAAGCAACTGATGGGTATCTTGGTGGGGTGTTTGAAATAGAGTCATTAGTGGAGAAATTGCTTCAGCAACTTGCTAGCAAGCAAACCGTGCTTGTTAATGTGTATGATACAACAAATCAGACACACCCAATTCCCATGTATGGTTCTGATGTATCAGGTGACGAGTTCTATCATGTGAGCTTTCTTAACTTTGGAGATCCTTTCAGGAAGCATGAGATGCACTGCAGGTTCAAACAGAAACCACCATTTCCAAGGTTGGCGACGGGTACATCAATTGGCATCCTTGTTATTGCACTCCTTGTTGGACATATTTGTCATGCAACTGTGAGTCGAATTGCCAAAGTAGAGGATGATTACCAAGAGATGATGGTGCTTAAAAAACGAGCTGAAGCAGCTGATGTTGCTAAATCCCAGTTCCTTGCTACTGTTTCGCACGAGATCAGAACTCCAATGAACGGTGTTTTAGGCAGTGGAAAAGCTCTGGTGTCAATTATAAATGAAGTTTTGGACCATGCAAAGATTGAATCTGGTAAGATAGAGCTCGAGGTTGTGCTGTTCGATATACGAGCAATTATGGACGATGTGTTATCACTATTTTCTGAGAAATCTCAAGGAAAAGGAGCTGAGTTAGCAATTTATGTCTCGGATCAGGTTCCTCACCAGCTTATAGGTGATCCTGGAAGGTTTCGACAAATAATTACAAATCTCATGGGTAACTCGATTAAGTTCACAGACAAAGGACATATCTTTGTGACTATCCATCTTGTTGAGGAGGTTTTCCATTCAATAGAAGTTGAGAAAAAATCTATTTCAAAAGATGCAGAACATACATTAAGTGGTTTCCCAGATGCTGATGGTCGTCGCAGCTGGGAAGGATTCAGAGCCTTCAGTCACGAGGGACCTCTTGGTTCTTTCTCGTCCACCTCCTCCAATGATCTCATCAGTTTGATTGTATCGGTAGAGGATACAGGCGATGGTATTCCTCTAGAGTCGCAGCCGATGATATTCACTCCATTCATGCAGGTAGGTCCATCCATCTCGAGAAAACACGGAGGAACTGGTATTGGTCTAAGCATTAGCAAGTGTTTAGTGGGCCTCATGAATGGAGAAATCGGATTTGTGAGCGAACCGAAGATTGGAAGCACTTTCACATTTACAGCTGTGTTCACCAATGCATGTCCTAATTCAAATGAGCTTCAAACTCAACAAATCAATAACCAACCTCATCCTGCAACTTCAGAGTTTAATGGTATGGCTGCACTAGTTATTGATCCGAGACCCGTTCGTGCTGAAGTGTCGAGATATCACATACAAAGGCTTGGCGTTCGTGTTGAAATAGTTTCAGATTTGAAGCAAGGTTTGTCCACCGTAACCGATGGGGATGCATCTATCAATATGGTCCTGATTGAACAAGAGGTTTGGGAAAGAGACTCGAGTATTTCATCGCACTTTGTGAATAACATTAGGAAAGTTGTTGAAGTTGATAAAGGAAAAGAGATTCCTCCAAAACTATTTATTCTCGTTAATTCTAGTAGTTCTTTTAGAGCGAGTTCTACGGCATCATGTCTTCATAATCCAACAGTTGTCACAAAACCTTTGAGAGCAAGTATGCTTGCGGCTTCACTACAAAGAGCAATGGGTGTTGGGAACAAAGGGAATCCTCGAAACGGAGAACATCAGGGTTTGTCTCTCAAGCATCTTCTCTCTGGGAGAAAGATTCTTATTGTAGATGATAATAGTGTGAATCGCACGGTAGCAGCTGGTGCTTTGAAAAAATATGGAGCTGGTGTGGTTTGTGTAAGCAGTGGAATAGAAGCCATCACTATGCTGAGGCCACCCCATCAATTTGATGCATGTTTCATGGATATTCAAATGCCTGAAATGGATGGATTTGAAGCTACGCGTCGAATTCGAGAGATCGAAAACAATGTGAAAGACAGAGAACTGTTTGTTCATCTACCCATTTTGGCTATGACCGCAGACGTGATGCAGGCTACACACCAGGAATGCTCTAAGTGTGGGATGGATGGATATGTTTCAAAACCATTTGAAGCTGAACAACTTTATAGAGAGGTTTCAAAGTTTTTCCAATCATCTTGA
Protein sequence

>MsaT015870.1

MDTKMGFLGDGGKMWLKCWEKILGSTCKIHQQYYQCIGSKRVRRKLWRKLLLAWVLGWFIVSLWIFCGINLHNTEKRKETLASMCDERARMLQDQFNVSMNHIQAMSILISTFHHAKNPSAIDQKTFAKYTERTAFERPLTSGVAYAVRVLQSEREQFEKQQGWSIKRMDTMEQNPVHEDDYVPDELEPSPIHEEYAPVIFAQDTISHVISIDVLSGKEDRENVLRARESGKGVLTAPFRLLKTNRLGVILTFAVYKRDLPSNATPNERIQATDGYLGGVFEIESLVEKLLQQLASKQTVLVNVYDTTNQTHPIPMYGSDVSGDEFYHVSFLNFGDPFRKHEMHCRFKQKPPFPRLATGTSIGILVIALLVGHICHATVSRIAKVEDDYQEMMVLKKRAEAADVAKSQFLATVSHEIRTPMNGVLGSGKALVSIINEVLDHAKIESGKIELEVVLFDIRAIMDDVLSLFSEKSQGKGAELAIYVSDQVPHQLIGDPGRFRQIITNLMGNSIKFTDKGHIFVTIHLVEEVFHSIEVEKKSISKDAEHTLSGFPDADGRRSWEGFRAFSHEGPLGSFSSTSSNDLISLIVSVEDTGDGIPLESQPMIFTPFMQVGPSISRKHGGTGIGLSISKCLVGLMNGEIGFVSEPKIGSTFTFTAVFTNACPNSNELQTQQINNQPHPATSEFNGMAALVIDPRPVRAEVSRYHIQRLGVRVEIVSDLKQGLSTVTDGDASINMVLIEQEVWERDSSISSHFVNNIRKVVEVDKGKEIPPKLFILVNSSSSFRASSTASCLHNPTVVTKPLRASMLAASLQRAMGVGNKGNPRNGEHQGLSLKHLLSGRKILIVDDNSVNRTVAAGALKKYGAGVVCVSSGIEAITMLRPPHQFDACFMDIQMPEMDGFEATRRIREIENNVKDRELFVHLPILAMTADVMQATHQECSKCGMDGYVSKPFEAEQLYREVSKFFQSS*