Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015888 | PNY06680.1 | 86.111 | 144 | 13 | 2 | 1 | 138 | 5 | 147 | 2.39e-75 | 233 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015888 | tr|A0A2K3NUG6|A0A2K3NUG6_TRIPR | 86.111 | 144 | 13 | 2 | 1 | 138 | 5 | 147 | 1.14e-75 | 233 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG015888 | TF | NF-YC |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015888 | MtrunA17_Chr3g0122571 | 91.667 | 132 | 10 | 1 | 1 | 132 | 18 | 148 | 3.19e-79 | 232 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG015888 | AT5G43250.1 | 57.463 | 134 | 49 | 4 | 3 | 134 | 2 | 129 | 8.39e-44 | 141 |
| MsaG015888 | AT1G08970.3 | 34.400 | 125 | 72 | 4 | 3 | 118 | 68 | 191 | 3.35e-11 | 59.7 |
| MsaG015888 | AT1G08970.4 | 34.400 | 125 | 72 | 4 | 3 | 118 | 68 | 191 | 3.35e-11 | 59.7 |
| MsaG015888 | AT1G08970.2 | 34.400 | 125 | 72 | 4 | 3 | 118 | 68 | 191 | 3.35e-11 | 59.7 |
| MsaG015888 | AT1G08970.1 | 34.400 | 125 | 72 | 4 | 3 | 118 | 68 | 191 | 3.35e-11 | 59.7 |
| MsaG015888 | AT3G12480.1 | 38.961 | 77 | 46 | 1 | 11 | 87 | 5 | 80 | 4.57e-11 | 59.7 |
Find 42 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCCATGGGTTCCGGTTC+AGG | 0.246040 | 3:-92056906 | None:intergenic |
| TCGAGTAGAAAATCACTTAT+TGG | 0.284969 | 3:-92056717 | None:intergenic |
| TCCGGTTCAGGTTCAGGTTC+CGG | 0.296003 | 3:-92056894 | None:intergenic |
| CAACTTCGGCTTCAGACTTT+CGG | 0.357492 | 3:-92056848 | None:intergenic |
| ATGGGTTCCGGTTCAGGTTC+AGG | 0.371834 | 3:-92056900 | None:intergenic |
| GAGTTCAGAAGCGTTGTTCT+TGG | 0.377847 | 3:+92056572 | MsaT015888.1:CDS |
| TATGCCTCCTCCATGGGTTC+CGG | 0.409906 | 3:-92056912 | None:intergenic |
| GGATCTGCCTAAATCTGTTC+CGG | 0.416357 | 3:+92056797 | MsaT015888.1:CDS |
| GCCGGAACCTGAACCTGAAC+CGG | 0.418474 | 3:+92056893 | MsaT015888.1:CDS |
| ATCTTCTTCACTCTACTCTT+CGG | 0.421448 | 3:-92056519 | None:intergenic |
| GTACCAACCGGAACAGATTT+AGG | 0.436259 | 3:-92056804 | None:intergenic |
| TCAGGTTCAGGTTCCGGCGG+TGG | 0.450466 | 3:-92056888 | None:intergenic |
| AGATCCGCAACCGCCGCCGC+TGG | 0.453492 | 3:-92056780 | None:intergenic |
| GTTCTTGGTGTCACGATCTA+CGG | 0.459375 | 3:+92056587 | MsaT015888.1:CDS |
| TAAACCAGCGGCGGCGGTTG+CGG | 0.474107 | 3:+92056776 | MsaT015888.1:CDS |
| ATCTGATAAACCAGCGGCGG+CGG | 0.487271 | 3:+92056770 | MsaT015888.1:CDS |
| CACAACCTATGCCTCCTCCA+TGG | 0.514005 | 3:-92056919 | None:intergenic |
| ACTCTACTCTTCGGAAACTC+TGG | 0.522689 | 3:-92056510 | None:intergenic |
| TCAATGCGGCGAGTACCAAC+CGG | 0.541474 | 3:-92056816 | None:intergenic |
| ACAACCTATGCCTCCTCCAT+GGG | 0.542610 | 3:-92056918 | None:intergenic |
| CTCTGGACAAAGACGTGAAG+AGG | 0.546039 | 3:+92056547 | MsaT015888.1:CDS |
| TTCGCAGTTTCCGACGGCAT+CGG | 0.549112 | 3:-92056747 | None:intergenic |
| AATTGCAATTGTTATGGCAG+AGG | 0.550560 | 3:+92056461 | None:intergenic |
| TTGCGCTTGAGCATCAACTT+CGG | 0.551263 | 3:-92056862 | None:intergenic |
| GGAACCCATGGAGGAGGCAT+AGG | 0.556067 | 3:+92056914 | MsaT015888.1:CDS |
| TCAGATTTCGCAGTTTCCGA+CGG | 0.560923 | 3:-92056753 | None:intergenic |
| GTAGAAAATCACTTATTGGA+CGG | 0.569952 | 3:-92056713 | None:intergenic |
| TGAACCTGAACCGGAACCCA+TGG | 0.579146 | 3:+92056902 | MsaT015888.1:CDS |
| TCAAGCGCAAGCGCCACCGC+CGG | 0.588095 | 3:+92056875 | MsaT015888.1:CDS |
| CTGCCTAAATCTGTTCCGGT+TGG | 0.595205 | 3:+92056801 | MsaT015888.1:CDS |
| AGTGAAGAAGATAATCACTC+TGG | 0.599750 | 3:+92056530 | MsaT015888.1:CDS |
| TGAACCGGAACCCATGGAGG+AGG | 0.608400 | 3:+92056908 | MsaT015888.1:CDS |
| TCTGGACAAAGACGTGAAGA+GGG | 0.629567 | 3:+92056548 | MsaT015888.1:CDS |
| CGATTCGCTTCCGATGCCGT+CGG | 0.664511 | 3:+92056737 | MsaT015888.1:CDS |
| ACCTGAACCGGAACCCATGG+AGG | 0.681479 | 3:+92056905 | MsaT015888.1:CDS |
| GGTTCAGGTTCAGGTTCCGG+CGG | 0.690810 | 3:-92056891 | None:intergenic |
| TATTGAGAAGAAGCGCAAGA+CGG | 0.692763 | 3:+92056653 | MsaT015888.1:CDS |
| GTTACTTGCTGAAAAGTCTG+CGG | 0.696867 | 3:+92056623 | MsaT015888.1:CDS |
| GCTTGAACATATAAGAATCG+CGG | 0.698334 | 3:+92056680 | MsaT015888.1:CDS |
| GAAATCTGATAAACCAGCGG+CGG | 0.702085 | 3:+92056767 | MsaT015888.1:CDS |
| TGCGAAATCTGATAAACCAG+CGG | 0.703898 | 3:+92056764 | MsaT015888.1:CDS |
| TTCGGAAGATCTGATCAATG+CGG | 0.759168 | 3:-92056830 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 92056474 | 92056935 | 92056474 | ID=MsaG015888 |
| Chr3 | mRNA | 92056474 | 92056935 | 92056474 | ID=MsaT015888.1;Parent=MsaG015888 |
| Chr3 | exon | 92056474 | 92056935 | 92056474 | ID=MsaT015888.1.exon1;Parent=MsaT015888.1 |
| Chr3 | CDS | 92056474 | 92056935 | 92056474 | ID=cds.MsaT015888.1;Parent=MsaT015888.1 |
| Gene Sequence |
| Protein sequence |
