AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016074

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016074 MtrunA17_Chr3g0124601 99.449 363 2 0 1 363 1 363 0.0 751
MsaG016074 MtrunA17_Chr8g0382861 68.839 353 102 4 12 363 13 358 1.69e-176 494
MsaG016074 MtrunA17_Chr8g0353501 56.269 327 125 4 43 361 150 466 3.41e-117 347
MsaG016074 MtrunA17_Chr3g0115371 49.429 350 170 3 14 359 1 347 4.49e-116 341
MsaG016074 MtrunA17_Chr7g0256211 58.667 300 111 3 70 361 158 452 9.80e-113 335
MsaG016074 MtrunA17_Chr5g0424861 49.716 352 165 4 14 358 1 347 2.04e-111 329
MsaG016074 MtrunA17_Chr5g0422771 55.556 306 124 3 64 361 163 464 6.36e-110 329
MsaG016074 MtrunA17_Chr2g0321431 54.795 292 120 4 73 361 187 469 6.63e-109 327
MsaG016074 MtrunA17_Chr1g0210151 55.401 287 124 2 79 361 54 340 1.27e-105 313
MsaG016074 MtrunA17_Chr1g0174701 52.117 307 138 3 64 361 200 506 1.18e-103 314
MsaG016074 MtrunA17_Chr4g0026401 46.991 349 155 6 30 361 126 461 1.79e-103 313
MsaG016074 MtrunA17_Chr7g0258321 50.321 312 148 2 57 361 152 463 6.77e-103 311
MsaG016074 MtrunA17_Chr5g0424851 53.704 216 99 1 144 358 7 222 2.16e-75 239
MsaG016074 MtrunA17_Chr5g0424851 53.960 202 93 0 148 349 225 426 6.67e-72 230
MsaG016074 MtrunA17_Chr3g0093451 57.669 163 58 3 82 235 49 209 1.32e-59 191
MsaG016074 MtrunA17_Chr1g0196951 38.806 201 98 5 160 354 59 240 7.16e-34 125
MsaG016074 MtrunA17_Chr8g0384821 25.957 235 139 5 150 349 14 248 7.80e-16 76.6
MsaG016074 MtrunA17_Chr7g0242651 24.651 215 152 5 147 351 5 219 1.24e-14 72.8
MsaG016074 MtrunA17_Chr2g0315631 23.182 220 135 3 151 350 4 209 2.09e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016074 AT5G65210.5 67.663 368 114 2 1 363 1 368 0.0 515
MsaG016074 AT5G65210.7 67.663 368 114 2 1 363 1 368 0.0 515
MsaG016074 AT5G65210.3 67.663 368 114 2 1 363 1 368 0.0 515
MsaG016074 AT5G65210.1 67.663 368 114 2 1 363 1 368 0.0 515
MsaG016074 AT5G65210.2 67.663 368 114 2 1 363 1 368 0.0 515
MsaG016074 AT5G65210.4 67.663 368 114 2 1 363 1 368 0.0 515
MsaG016074 AT5G10030.5 68.767 365 111 2 1 363 1 364 0.0 512
MsaG016074 AT5G10030.3 68.767 365 111 2 1 363 1 364 0.0 512
MsaG016074 AT5G10030.1 68.767 365 111 2 1 363 1 364 0.0 512
MsaG016074 AT5G10030.4 68.767 365 111 2 1 363 1 364 0.0 512
MsaG016074 AT5G10030.2 68.767 365 111 2 1 363 1 364 0.0 512
MsaG016074 AT5G65210.6 73.064 297 78 1 69 363 2 298 1.67e-160 452
MsaG016074 AT1G77920.1 56.764 377 136 7 2 363 4 368 8.89e-143 409
MsaG016074 AT1G22070.1 56.557 366 143 4 12 361 17 382 1.93e-135 391
MsaG016074 AT5G06950.2 59.516 289 107 2 79 361 46 330 2.16e-113 333
MsaG016074 AT5G06950.4 59.516 289 107 2 79 361 46 330 2.16e-113 333
MsaG016074 AT5G06950.1 59.516 289 107 2 79 361 46 330 2.16e-113 333
MsaG016074 AT5G06950.5 59.516 289 107 2 79 361 46 330 2.16e-113 333
MsaG016074 AT5G06950.3 59.516 289 107 2 79 361 46 330 2.16e-113 333
MsaG016074 AT3G12250.6 54.268 328 131 4 49 361 7 330 2.13e-112 331
MsaG016074 AT3G12250.2 54.268 328 131 4 49 361 7 330 2.13e-112 331
MsaG016074 AT3G12250.7 54.268 328 131 4 49 361 7 330 2.13e-112 331
MsaG016074 AT3G12250.1 54.268 328 131 4 49 361 7 330 2.13e-112 331
MsaG016074 AT3G12250.3 55.305 311 129 2 57 361 18 324 1.93e-111 328
MsaG016074 AT3G12250.5 57.191 299 114 3 73 361 9 303 3.57e-111 327
MsaG016074 AT5G06960.3 58.824 289 109 2 79 361 26 310 8.14e-111 326
MsaG016074 AT5G06960.2 58.824 289 109 2 79 361 46 330 9.62e-111 327
MsaG016074 AT5G06960.1 58.824 289 109 2 79 361 46 330 9.62e-111 327
MsaG016074 AT3G12250.4 55.305 311 129 2 57 361 49 355 1.38e-110 327
MsaG016074 AT1G08320.2 56.098 287 111 3 79 361 54 329 9.75e-110 325
MsaG016074 AT1G08320.5 56.098 287 111 3 79 361 54 329 9.75e-110 325
MsaG016074 AT1G08320.1 56.098 287 111 3 79 361 178 453 3.36e-108 325
MsaG016074 AT1G08320.4 56.098 287 111 3 79 361 178 453 3.36e-108 325
MsaG016074 AT1G08320.3 56.098 287 111 3 79 361 178 453 3.36e-108 325
MsaG016074 AT5G06839.1 49.538 325 147 4 52 361 93 415 4.68e-102 307
MsaG016074 AT5G06839.2 49.387 326 147 4 52 361 93 416 7.20e-102 307
MsaG016074 AT5G06839.3 52.685 298 126 3 79 361 161 458 1.13e-101 308
MsaG016074 AT1G68640.1 52.922 308 136 3 61 361 147 452 3.67e-99 301
MsaG016074 AT1G58330.1 26.126 222 151 4 143 351 2 223 1.04e-18 84.3
MsaG016074 AT3G14880.2 26.126 222 129 7 147 343 6 217 1.04e-12 67.4
MsaG016074 AT3G14880.1 25.328 229 129 7 147 343 6 224 7.70e-12 65.1

Find 84 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTCGATGTTTGCTTGCTT+TGG 0.213054 3:+93927560 MsaT016074.1:CDS
TTGATTTAATTGCAGATTCT+TGG 0.226068 3:+93926958 MsaT016074.1:intron
AAAACTGCAGGGAATGTATA+TGG 0.249625 3:+93926311 MsaT016074.1:intron
CAAGTCCGCCTCATCAATTA+AGG 0.315142 3:-93925653 None:intergenic
TTCTCATTTCCGAGATTTGC+CGG 0.317139 3:-93926537 None:intergenic
GCAGCCTTTGAGATGGAGTA+TGG 0.346430 3:+93926493 MsaT016074.1:CDS
GCTCGATGTTTGCTTGCTTT+GGG 0.348084 3:+93927561 MsaT016074.1:CDS
CATGTTCTAACCTTATCAAT+AGG 0.384759 3:-93925851 None:intergenic
TCTGTCAATGGTTCAATCAT+TGG 0.390810 3:-93926982 None:intergenic
CATACTTGTTAGATGTTCCT+AGG 0.414801 3:-93925811 None:intergenic
GGCTCTTACGAGCAGCTTCT+CGG 0.427961 3:-93925997 None:intergenic
CTTCTCCCCACATATTAATC+TGG 0.429966 3:-93925594 None:intergenic
CACATATTAATCTGGTGAAT+AGG 0.436108 3:-93925586 None:intergenic
TTTAACAGGCTGACCATCTT+CGG 0.437689 3:+93927490 MsaT016074.1:intron
AACAAGACTAAGCAGGTTCT+CGG 0.440007 3:-93927635 None:intergenic
ATTGGGTTGATGAGCAAAAC+CGG 0.440031 3:+93926518 MsaT016074.1:CDS
AATGTATATGGGTGGTGGGT+TGG 0.443309 3:+93926323 MsaT016074.1:CDS
ACAAGACTAAGCAGGTTCTC+GGG 0.443620 3:-93927634 None:intergenic
CTTGAAGTTTGATAGTAGTC+AGG 0.454381 3:+93925672 MsaT016074.1:CDS
TTCGATAGATTTCAGCATAG+TGG 0.456188 3:-93926621 None:intergenic
AATCGTTGTTGCTCTGTCAA+TGG 0.456266 3:-93926994 None:intergenic
TCTTGTGAGCTTTGTGAACC+AGG 0.458406 3:+93927179 MsaT016074.1:CDS
AGCTCAAAGCCCTCAGGCGT+TGG 0.459290 3:-93927593 None:intergenic
GAGCTCAGGATGCTGGTTGA+TGG 0.463581 3:+93926589 MsaT016074.1:CDS
TGAGATGGAGTATGGACATT+GGG 0.465713 3:+93926501 MsaT016074.1:CDS
CACAGTTTGTTTCCTCGAGA+AGG 0.467650 3:+93925548 MsaT016074.1:CDS
TGAGGGCTTTGAGCTCACTA+TGG 0.474812 3:+93927601 MsaT016074.1:CDS
TCGTTTGAAGTTAGTGCAGT+TGG 0.476876 3:+93926153 MsaT016074.1:CDS
TTTGCTGGACCTGTGAATCC+AGG 0.482156 3:+93926363 MsaT016074.1:CDS
CGCCTCATCAATTAAGGGAA+TGG 0.487369 3:-93925647 None:intergenic
GTTTCTCCATGCCTTGTGAA+AGG 0.488025 3:-93927062 None:intergenic
GATGCTTCTCATGGTATCCT+AGG 0.490549 3:+93925794 MsaT016074.1:CDS
GCAAGGTTTCCTGCCGAAGA+TGG 0.491320 3:-93927503 None:intergenic
TTTCTCCATGCCTTGTGAAA+GGG 0.491745 3:-93927061 None:intergenic
AGCTAATAGACCTATTGATA+AGG 0.501170 3:+93925841 MsaT016074.1:CDS
CTCGCTGATAGCGTAGCAGC+TGG 0.502070 3:+93927096 MsaT016074.1:CDS
CAGGGAATGTATATGGGTGG+TGG 0.504131 3:+93926318 MsaT016074.1:intron
AAGTCCGCCTCATCAATTAA+GGG 0.508625 3:-93925652 None:intergenic
ATTGACAGAGCAACAACGAT+TGG 0.518816 3:+93926996 MsaT016074.1:CDS
ACAAACCAACCTGGATTCAC+AGG 0.524884 3:-93926372 None:intergenic
TCGCCCTTCTGAGCTTCTCA+AGG 0.526161 3:+93926738 MsaT016074.1:CDS
TGACATAGAGCTCAGGATGC+TGG 0.526532 3:+93926582 MsaT016074.1:CDS
ACAGGCTGACCATCTTCGGC+AGG 0.532331 3:+93927494 MsaT016074.1:intron
CAGATTAATATGTGGGGAGA+AGG 0.534968 3:+93925595 MsaT016074.1:CDS
ATCACCTTGAGAAGCTCAGA+AGG 0.537726 3:-93926742 None:intergenic
TCCACAGATGGCTACTGCAA+TGG 0.537758 3:+93927143 MsaT016074.1:CDS
AGAATATTTCCAACGCCTGA+GGG 0.537877 3:+93927584 MsaT016074.1:CDS
AGGAATTGCAGCCTTTGAGA+TGG 0.538267 3:+93926486 MsaT016074.1:intron
AAACTGCAGGGAATGTATAT+GGG 0.538541 3:+93926312 MsaT016074.1:intron
TGAGCAAAACCGGCAAATCT+CGG 0.539595 3:+93926528 MsaT016074.1:CDS
AGATGCCCTTTCACAAGGCA+TGG 0.540096 3:+93927056 MsaT016074.1:CDS
GCTCGAACGTGTTAGACAGC+AGG 0.550918 3:+93926183 MsaT016074.1:CDS
TATTCACCAGATTAATATGT+GGG 0.557449 3:+93925588 MsaT016074.1:CDS
TGTTCGCGACATTTGTTGCA+AGG 0.557667 3:-93927520 None:intergenic
TTGAGATGGAGTATGGACAT+TGG 0.559206 3:+93926500 MsaT016074.1:CDS
CTATTCACCAGATTAATATG+TGG 0.559375 3:+93925587 MsaT016074.1:CDS
ATAGTGAGCTCAAAGCCCTC+AGG 0.562814 3:-93927599 None:intergenic
GCAGCTGGACAATTCATTGA+AGG 0.563520 3:+93927111 MsaT016074.1:CDS
AGGGAATGTATATGGGTGGT+GGG 0.568452 3:+93926319 MsaT016074.1:intron
GAGAATATTTCCAACGCCTG+AGG 0.568747 3:+93927583 MsaT016074.1:CDS
GGAGAAGGCTTCAAAAGTAA+TGG 0.571903 3:+93925610 MsaT016074.1:CDS
CTTGTTGACATGATTGTCCA+AGG 0.580404 3:-93927029 None:intergenic
TCCATTGCAGTAGCCATCTG+TGG 0.581181 3:-93927144 None:intergenic
ATCCATTCCCTTAATTGATG+AGG 0.582525 3:+93925645 MsaT016074.1:CDS
TAGTCAGAGGATGCTTCTCA+TGG 0.584672 3:+93925785 MsaT016074.1:intron
CGATTGGATATCGATAACCT+TGG 0.584683 3:+93927012 MsaT016074.1:CDS
AGGAACTTATATTCCACAGA+TGG 0.586141 3:+93927131 MsaT016074.1:CDS
CATTCCCTTAATTGATGAGG+CGG 0.591538 3:+93925648 MsaT016074.1:CDS
GTATTAGTGACATAGAGCTC+AGG 0.591773 3:+93926575 MsaT016074.1:CDS
GCAGAAGATGCCCTTTCACA+AGG 0.607214 3:+93927051 MsaT016074.1:CDS
GTCTTGCAACAAGACTAAGC+AGG 0.618652 3:-93927642 None:intergenic
CAAGACTAAGCAGGTTCTCG+GGG 0.624393 3:-93927633 None:intergenic
GCAAACATCGAGCTGACTGA+CGG 0.633030 3:-93927551 None:intergenic
GATTAAATATGCAGATACAA+AGG 0.638966 3:+93925960 MsaT016074.1:intron
GAGGAAACAAACTGTGCGGA+CGG 0.640938 3:-93925541 None:intergenic
ATGTCCATACTCCATCTCAA+AGG 0.641308 3:-93926497 None:intergenic
TTTATGTGATGTCCGGCATG+TGG 0.642596 3:+93926674 MsaT016074.1:CDS
ATACACACTCATCCTTCTCG+AGG 0.650671 3:-93925560 None:intergenic
CACAAGGCATGGAGAAACTG+CGG 0.659798 3:+93927067 MsaT016074.1:CDS
TCACCTTGAGAAGCTCAGAA+GGG 0.664994 3:-93926741 None:intergenic
CTGCAGGGAATGTATATGGG+TGG 0.681880 3:+93926315 MsaT016074.1:intron
ATTCACCAGATTAATATGTG+GGG 0.681960 3:+93925589 MsaT016074.1:CDS
GTCCGGCATGTGGAAAACAA+CGG 0.755517 3:+93926684 MsaT016074.1:CDS
TCTCGAGGAAACAAACTGTG+CGG 0.757278 3:-93925545 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 93925532 93927650 93925532 ID=MsaG016074
Chr3 mRNA 93925532 93927650 93925532 ID=MsaT016074.1;Parent=MsaG016074
Chr3 exon 93925532 93925693 93925532 ID=MsaT016074.1.exon1;Parent=MsaT016074.1
Chr3 CDS 93925532 93925693 93925532 ID=cds.MsaT016074.1;Parent=MsaT016074.1
Chr3 exon 93925788 93925862 93925788 ID=MsaT016074.1.exon2;Parent=MsaT016074.1
Chr3 CDS 93925788 93925862 93925788 ID=cds.MsaT016074.1;Parent=MsaT016074.1
Chr3 exon 93925974 93926033 93925974 ID=MsaT016074.1.exon3;Parent=MsaT016074.1
Chr3 CDS 93925974 93926033 93925974 ID=cds.MsaT016074.1;Parent=MsaT016074.1
Chr3 exon 93926127 93926204 93926127 ID=MsaT016074.1.exon4;Parent=MsaT016074.1
Chr3 CDS 93926127 93926204 93926127 ID=cds.MsaT016074.1;Parent=MsaT016074.1
Chr3 exon 93926321 93926384 93926321 ID=MsaT016074.1.exon5;Parent=MsaT016074.1
Chr3 CDS 93926321 93926384 93926321 ID=cds.MsaT016074.1;Parent=MsaT016074.1
Chr3 exon 93926488 93926759 93926488 ID=MsaT016074.1.exon6;Parent=MsaT016074.1
Chr3 CDS 93926488 93926759 93926488 ID=cds.MsaT016074.1;Parent=MsaT016074.1
Chr3 exon 93926973 93927200 93926973 ID=MsaT016074.1.exon7;Parent=MsaT016074.1
Chr3 CDS 93926973 93927200 93926973 ID=cds.MsaT016074.1;Parent=MsaT016074.1
Chr3 exon 93927498 93927650 93927498 ID=MsaT016074.1.exon8;Parent=MsaT016074.1
Chr3 CDS 93927498 93927650 93927498 ID=cds.MsaT016074.1;Parent=MsaT016074.1
Gene Sequence

>MsaT016074.1

ATGAATTCGCCGTCCGCACAGTTTGTTTCCTCGAGAAGGATGAGTGTGTATGATCCTATTCACCAGATTAATATGTGGGGAGAAGGCTTCAAAAGTAATGGAAATTTAAGTGCATCCATTCCCTTAATTGATGAGGCGGACTTGAAGTTTGATAGTAGTCAGTCAGAGGATGCTTCTCATGGTATCCTAGGAACATCTAACAAGTATGAGCAAGAAGCTAATAGACCTATTGATAAGATACAAAGGCGTCTTGCACAAAACCGAGAAGCTGCTCGTAAGAGCCGTCTGCGAAAAAAGGCTTATGTGCAGCAGCTAGAATCAAGTCGTTTGAAGTTAGTGCAGTTGGAGCAAGAGCTCGAACGTGTTAGACAGCAGGGAATGTATATGGGTGGTGGGTTGGATTCTAATAATATGTGTTTTGCTGGACCTGTGAATCCAGGAATTGCAGCCTTTGAGATGGAGTATGGACATTGGGTTGATGAGCAAAACCGGCAAATCTCGGAAATGAGAAATGCTTTAAACTCGCGTATTAGTGACATAGAGCTCAGGATGCTGGTTGATGGTATGATGAACCACTATGCTGAAATCTATCGAATGAAATCTGCAGCTGCAAAAACAGATGTTTTTTATGTGATGTCCGGCATGTGGAAAACAACGGCTGAAAGATTTTTCTTGTGGATCGGTGGATTTCGCCCTTCTGAGCTTCTCAAGATTCTTGGTCCAATGATTGAACCATTGACAGAGCAACAACGATTGGATATCGATAACCTTGGACAATCATGTCAACAAGCAGAAGATGCCCTTTCACAAGGCATGGAGAAACTGCGGCAAACACTCGCTGATAGCGTAGCAGCTGGACAATTCATTGAAGGAACTTATATTCCACAGATGGCTACTGCAATGGAGAAGTTAGAAGCTCTTGTGAGCTTTGTGAACCAGGCTGACCATCTTCGGCAGGAAACCTTGCAACAAATGTCGCGAACACTTACAATCCGTCAGTCAGCTCGATGTTTGCTTGCTTTGGGAGAATATTTCCAACGCCTGAGGGCTTTGAGCTCACTATGGTCTAATCGACCCCGAGAACCTGCTTAG
Protein sequence

>MsaT016074.1

MNSPSAQFVSSRRMSVYDPIHQINMWGEGFKSNGNLSASIPLIDEADLKFDSSQSEDASHGILGTSNKYEQEANRPIDKIQRRLAQNREAARKSRLRKKAYVQQLESSRLKLVQLEQELERVRQQGMYMGGGLDSNNMCFAGPVNPGIAAFEMEYGHWVDEQNRQISEMRNALNSRISDIELRMLVDGMMNHYAEIYRMKSAAAKTDVFYVMSGMWKTTAERFFLWIGGFRPSELLKILGPMIEPLTEQQRLDIDNLGQSCQQAEDALSQGMEKLRQTLADSVAAGQFIEGTYIPQMATAMEKLEALVSFVNQADHLRQETLQQMSRTLTIRQSARCLLALGEYFQRLRALSSLWSNRPREPA*