AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016083

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016083 MtrunA17_Chr3g0124761 95.299 234 5 2 1 228 55 288 1.10e-166 461
MsaG016083 MtrunA17_Chr8g0384721 60.082 243 68 7 1 226 55 285 3.57e-86 256
MsaG016083 MtrunA17_Chr3g0130731 56.917 253 69 6 1 228 55 292 6.84e-78 236
MsaG016083 MtrunA17_Chr2g0318101 61.224 147 37 5 2 137 64 201 3.37e-51 169
MsaG016083 MtrunA17_Chr7g0252971 61.364 132 40 2 2 122 57 188 3.31e-49 164
MsaG016083 MtrunA17_Chr8g0359921 71.818 110 29 2 2 110 63 171 4.14e-48 160
MsaG016083 MtrunA17_Chr2g0318081 70.642 109 28 2 2 109 65 170 1.06e-47 160
MsaG016083 MtrunA17_Chr4g0047841 63.566 129 22 3 2 106 64 191 1.66e-45 154
MsaG016083 MtrunA17_Chr2g0326361 61.207 116 41 2 4 115 65 180 6.35e-45 152
MsaG016083 MtrunA17_Chr5g0417791 56.618 136 51 3 2 129 52 187 3.68e-44 149
MsaG016083 MtrunA17_Chr7g0263931 63.303 109 36 2 2 106 60 168 4.79e-42 145
MsaG016083 MtrunA17_Chr4g0042561 64.762 105 35 2 2 104 58 162 1.86e-41 142
MsaG016083 MtrunA17_Chr6g0453451 53.636 110 49 2 2 110 69 177 3.21e-35 127
MsaG016083 MtrunA17_Chr3g0139331 46.309 149 64 4 2 141 60 201 3.75e-35 127
MsaG016083 MtrunA17_Chr6g0484441 55.963 109 42 2 2 109 54 157 4.66e-34 124
MsaG016083 MtrunA17_Chr5g0417001 44.286 140 70 2 1 132 62 201 8.64e-34 125
MsaG016083 MtrunA17_Chr2g0309331 39.535 172 77 5 6 162 66 225 1.04e-33 124
MsaG016083 MtrunA17_Chr3g0113001 50.450 111 54 1 1 110 13 123 1.60e-33 122
MsaG016083 MtrunA17_Chr1g0147421 53.448 116 42 3 2 112 77 185 3.61e-33 122
MsaG016083 MtrunA17_Chr4g0060931 54.128 109 48 2 2 109 66 173 8.57e-33 121
MsaG016083 MtrunA17_Chr3g0091921 52.066 121 50 3 2 121 65 178 9.80e-33 120
MsaG016083 MtrunA17_Chr7g0253011 53.982 113 46 2 2 109 66 177 9.80e-33 122
MsaG016083 MtrunA17_Chr6g0453131 49.565 115 50 2 1 107 63 177 1.34e-32 121
MsaG016083 MtrunA17_Chr4g0076731 53.509 114 45 3 1 112 102 209 1.69e-32 122
MsaG016083 MtrunA17_Chr6g0481091 45.185 135 58 4 8 129 63 194 5.01e-32 117
MsaG016083 MtrunA17_Chr2g0316971 55.963 109 42 3 2 106 72 178 5.15e-32 120
MsaG016083 MtrunA17_Chr2g0309891 50.000 126 56 5 6 130 61 180 5.58e-32 119
MsaG016083 MtrunA17_Chr8g0361731 41.892 148 67 3 1 134 62 204 8.57e-32 119
MsaG016083 MtrunA17_Chr5g0433781 56.311 103 41 3 6 107 61 160 1.38e-31 121
MsaG016083 MtrunA17_Chr4g0017921 53.333 105 42 2 1 104 59 157 2.35e-31 117
MsaG016083 MtrunA17_Chr3g0109821 56.436 101 41 2 8 107 63 161 2.59e-31 120
MsaG016083 MtrunA17_Chr6g0461661 56.190 105 42 2 2 103 67 170 3.26e-31 117
MsaG016083 MtrunA17_Chr2g0312321 37.436 195 96 6 2 195 58 227 4.06e-31 116
MsaG016083 MtrunA17_Chr8g0372121 46.364 110 53 2 6 114 62 166 5.71e-30 113
MsaG016083 MtrunA17_Chr5g0428991 56.000 100 36 3 8 104 82 176 1.48e-29 115
MsaG016083 MtrunA17_Chr4g0038751 46.825 126 54 4 1 122 63 179 1.50e-29 112
MsaG016083 MtrunA17_Chr7g0217721 45.161 124 51 3 1 109 67 188 1.72e-29 112
MsaG016083 MtrunA17_Chr7g0217711 59.259 81 32 1 1 80 67 147 4.75e-29 107
MsaG016083 MtrunA17_Chr7g0214071 51.786 112 46 2 6 114 65 171 1.18e-28 108
MsaG016083 MtrunA17_Chr4g0056541 47.664 107 50 2 1 106 58 159 1.27e-28 110
MsaG016083 MtrunA17_Chr4g0018391 48.039 102 47 2 6 106 63 159 1.96e-28 109
MsaG016083 MtrunA17_Chr8g0346001 45.161 124 57 3 6 118 71 194 2.16e-28 110
MsaG016083 MtrunA17_Chr5g0398971 47.170 106 50 2 1 104 58 159 3.54e-28 109
MsaG016083 MtrunA17_Chr1g0183741 49.541 109 45 2 1 106 58 159 6.19e-28 107
MsaG016083 MtrunA17_Chr5g0400461 51.887 106 37 4 6 103 61 160 6.73e-28 108
MsaG016083 MtrunA17_Chr3g0144271 50.962 104 46 2 1 101 72 173 7.43e-28 109
MsaG016083 MtrunA17_Chr3g0128151 54.545 99 37 3 8 103 79 172 8.69e-28 110
MsaG016083 MtrunA17_Chr8g0364791 48.039 102 49 2 5 105 60 158 9.40e-28 109
MsaG016083 MtrunA17_Chr3g0130101 48.039 102 49 2 5 105 61 159 3.33e-27 106
MsaG016083 MtrunA17_Chr5g0405211 44.915 118 53 3 6 122 64 170 3.66e-27 106
MsaG016083 MtrunA17_Chr5g0442771 44.444 126 60 3 2 117 67 192 4.35e-27 105
MsaG016083 MtrunA17_Chr7g0261411 49.524 105 43 2 1 102 58 155 1.16e-26 103
MsaG016083 MtrunA17_Chr1g0197361 50.000 100 45 3 8 106 66 161 1.18e-26 107
MsaG016083 MtrunA17_Chr1g0200561 47.475 99 46 2 6 103 75 168 4.66e-26 103
MsaG016083 MtrunA17_Chr1g0201021 48.544 103 47 3 5 105 73 171 1.08e-25 104
MsaG016083 MtrunA17_Chr3g0092671 49.000 100 46 3 8 106 63 158 1.25e-25 104
MsaG016083 MtrunA17_Chr3g0128111 48.485 99 43 3 8 103 99 192 2.29e-25 99.8
MsaG016083 MtrunA17_Chr1g0197351 50.000 102 47 2 6 103 97 198 2.49e-25 103
MsaG016083 MtrunA17_Chr8g0384201 45.098 102 52 3 5 105 60 158 4.63e-25 102
MsaG016083 MtrunA17_Chr2g0311651 33.649 211 109 7 5 187 75 282 5.94e-25 99.8
MsaG016083 MtrunA17_Chr3g0131911 48.182 110 45 3 5 104 64 171 1.06e-24 97.8
MsaG016083 MtrunA17_Chr2g0321741 48.958 96 38 3 6 100 46 131 1.56e-24 99.4
MsaG016083 MtrunA17_Chr4g0054791 49.000 100 44 2 8 103 63 159 3.15e-24 98.2
MsaG016083 MtrunA17_Chr3g0113031 48.485 99 45 3 16 110 3 99 4.07e-24 97.1
MsaG016083 MtrunA17_Chr8g0385991 44.231 104 53 2 6 104 65 168 5.14e-24 95.5
MsaG016083 MtrunA17_Chr2g0311661 44.538 119 53 3 5 110 74 192 3.50e-23 94.7
MsaG016083 MtrunA17_Chr7g0243371 57.333 75 29 1 6 77 17 91 4.13e-23 90.1
MsaG016083 MtrunA17_Chr4g0026141 34.756 164 83 5 6 146 63 225 3.16e-21 89.0
MsaG016083 MtrunA17_Chr2g0321631 43.269 104 55 3 7 107 64 166 8.00e-21 87.8
MsaG016083 MtrunA17_Chr8g0345671 47.917 96 38 3 8 100 69 155 3.10e-20 88.2
MsaG016083 MtrunA17_Chr7g0266701 34.959 123 70 4 7 120 64 185 2.23e-19 84.0
MsaG016083 MtrunA17_Chr8g0345691 39.583 96 46 3 8 100 70 156 2.10e-15 74.3
MsaG016083 MtrunA17_Chr8g0345721 45.205 73 36 2 6 75 120 191 1.34e-14 70.1
MsaG016083 MtrunA17_Chr8g0345741 44.304 79 40 2 6 81 47 124 1.53e-14 68.6
MsaG016083 MtrunA17_Chr4g0050701 37.255 102 50 3 8 103 66 159 4.91e-13 65.5
MsaG016083 MtrunA17_Chr8g0345711 41.096 73 39 2 6 75 68 139 3.48e-12 62.8
MsaG016083 MtrunA17_Chr8g0345731 43.421 76 38 2 8 78 82 157 4.36e-12 62.4
MsaG016083 MtrunA17_Chr8g0384351 30.469 128 66 5 2 128 58 163 8.64e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016083 AT1G01720.1 66.529 242 60 7 1 228 55 289 3.24e-106 308
MsaG016083 AT5G08790.1 63.547 203 41 6 1 195 55 232 2.47e-75 229
MsaG016083 AT1G77450.1 59.809 209 40 5 1 206 58 225 1.07e-74 227
MsaG016083 AT5G63790.1 52.340 235 63 6 1 228 98 290 3.28e-68 212
MsaG016083 AT5G63790.2 52.340 235 63 6 1 228 108 300 4.16e-68 212
MsaG016083 AT3G15500.1 70.796 113 29 2 2 113 63 172 1.22e-48 162
MsaG016083 AT1G52890.1 71.818 110 27 2 2 110 63 169 2.33e-48 161
MsaG016083 AT4G27410.2 71.818 110 27 2 2 110 63 169 1.36e-47 159
MsaG016083 AT1G61110.1 68.966 116 25 2 2 106 65 180 1.53e-47 159
MsaG016083 AT4G27410.3 71.818 110 27 2 2 110 80 186 1.88e-47 159
MsaG016083 AT3G04070.2 59.333 150 43 6 2 136 59 205 7.05e-46 155
MsaG016083 AT3G04070.1 54.545 165 43 6 2 136 59 221 1.73e-45 155
MsaG016083 AT3G15510.1 63.158 133 34 5 2 122 66 195 2.58e-45 155
MsaG016083 AT1G69490.1 68.571 105 31 2 4 106 60 164 9.15e-45 150
MsaG016083 AT1G52880.1 69.565 115 25 2 2 106 66 180 7.03e-42 145
MsaG016083 AT5G61430.2 53.390 118 41 3 1 111 1 111 1.22e-34 125
MsaG016083 AT2G02450.1 55.263 114 43 3 1 112 99 206 2.59e-34 126
MsaG016083 AT2G02450.2 55.263 114 43 3 1 112 99 206 2.76e-34 127
MsaG016083 AT5G61430.1 55.357 112 48 2 1 111 64 174 5.80e-34 124
MsaG016083 AT1G76420.1 55.556 108 41 3 1 105 70 173 5.83e-34 124
MsaG016083 AT5G18270.2 56.034 116 47 3 2 114 70 184 6.46e-34 124
MsaG016083 AT5G18270.1 56.034 116 47 3 2 114 70 184 6.67e-34 124
MsaG016083 AT5G39610.1 53.390 118 53 2 2 118 69 185 7.77e-34 123
MsaG016083 AT5G07680.2 55.856 111 47 2 2 111 52 161 1.25e-33 123
MsaG016083 AT5G07680.1 55.856 111 47 2 2 111 66 175 1.42e-33 123
MsaG016083 AT3G18400.1 55.046 109 44 2 2 109 54 158 1.53e-33 123
MsaG016083 AT3G04060.1 58.095 105 41 2 1 103 68 171 3.27e-33 122
MsaG016083 AT2G33480.3 57.000 100 39 2 6 104 8 104 3.86e-33 119
MsaG016083 AT2G33480.2 59.000 100 35 2 6 104 65 159 5.24e-33 120
MsaG016083 AT3G15170.1 52.679 112 49 2 2 110 69 179 5.91e-33 121
MsaG016083 AT5G39820.1 44.361 133 53 3 1 112 68 200 5.91e-32 119
MsaG016083 AT3G17730.1 47.826 115 56 3 8 121 63 174 7.11e-32 117
MsaG016083 AT4G10350.2 54.286 105 44 2 1 104 13 114 8.43e-32 118
MsaG016083 AT1G56010.1 52.885 104 48 1 1 103 1 104 8.70e-32 117
MsaG016083 AT2G33480.1 57.000 100 39 2 6 104 65 161 8.83e-32 117
MsaG016083 AT2G24430.2 52.632 114 41 3 2 109 65 171 1.06e-31 118
MsaG016083 AT2G24430.1 52.632 114 41 3 2 109 65 171 1.06e-31 118
MsaG016083 AT1G26870.1 45.324 139 63 3 1 130 64 198 1.56e-31 119
MsaG016083 AT1G79580.3 53.465 101 39 2 6 104 73 167 2.09e-31 118
MsaG016083 AT1G79580.5 53.465 101 39 2 6 104 73 167 2.09e-31 118
MsaG016083 AT1G79580.4 53.465 101 39 2 6 104 73 167 2.09e-31 118
MsaG016083 AT1G79580.2 53.465 101 39 2 6 104 73 167 2.09e-31 118
MsaG016083 AT1G79580.1 53.465 101 39 2 6 104 73 167 2.09e-31 118
MsaG016083 AT4G10350.1 54.286 105 44 2 1 104 60 161 2.77e-31 117
MsaG016083 AT1G56010.2 52.885 104 48 1 1 103 68 171 3.41e-31 117
MsaG016083 AT1G33280.1 53.333 105 42 2 1 104 59 157 7.69e-31 115
MsaG016083 AT5G53950.1 53.153 111 36 3 2 103 66 169 9.89e-31 117
MsaG016083 AT4G35580.3 58.586 99 33 3 8 103 66 159 1.22e-30 118
MsaG016083 AT4G35580.2 58.586 99 33 3 8 103 66 159 1.23e-30 118
MsaG016083 AT2G43000.1 50.442 113 52 3 1 112 66 175 1.37e-30 114
MsaG016083 AT1G65910.1 52.427 103 45 3 8 109 63 162 1.70e-30 118
MsaG016083 AT4G35580.1 58.586 99 33 3 8 103 66 159 1.83e-30 117
MsaG016083 AT3G03200.1 51.351 111 48 4 6 114 61 167 5.59e-30 116
MsaG016083 AT3G12977.2 52.427 103 46 3 2 103 14 114 7.06e-30 111
MsaG016083 AT3G29035.1 70.130 77 22 1 2 77 73 149 9.64e-30 113
MsaG016083 AT3G10500.1 54.000 100 42 2 8 106 66 162 1.59e-29 115
MsaG016083 AT5G62380.2 52.941 102 42 2 6 106 63 159 2.07e-29 112
MsaG016083 AT5G62380.1 52.941 102 42 2 6 106 63 159 2.07e-29 112
MsaG016083 AT5G17260.1 47.009 117 56 4 6 120 61 173 2.12e-29 114
MsaG016083 AT5G04410.1 54.000 100 42 2 8 106 66 162 3.85e-29 114
MsaG016083 AT5G24590.2 45.455 121 57 3 6 122 68 183 4.72e-29 113
MsaG016083 AT1G33060.2 54.455 101 38 3 6 103 79 174 4.79e-29 114
MsaG016083 AT5G13180.1 55.882 102 38 2 6 104 64 161 4.98e-29 109
MsaG016083 AT1G33060.1 54.455 101 38 3 6 103 79 174 5.09e-29 114
MsaG016083 AT3G12977.1 52.427 103 46 3 2 103 67 167 6.22e-29 110
MsaG016083 AT4G17980.1 52.525 99 43 2 8 103 63 160 6.45e-29 109
MsaG016083 AT4G17980.2 52.525 99 43 2 8 103 63 160 8.65e-29 110
MsaG016083 AT4G28530.1 52.830 106 40 2 1 103 74 172 1.39e-28 110
MsaG016083 AT2G46770.1 48.182 110 46 3 6 104 72 181 1.87e-28 110
MsaG016083 AT3G10480.1 52.525 99 44 2 6 103 82 178 2.22e-28 111
MsaG016083 AT3G10480.2 51.515 99 44 2 6 103 82 177 2.74e-28 111
MsaG016083 AT3G10480.3 52.525 99 44 2 6 103 115 211 2.76e-28 112
MsaG016083 AT1G32770.1 34.158 202 106 4 1 175 67 268 2.77e-28 110
MsaG016083 AT2G17040.1 48.214 112 46 3 1 108 54 157 3.57e-28 108
MsaG016083 AT5G46590.1 53.000 100 43 3 6 103 61 158 6.81e-28 107
MsaG016083 AT1G62700.1 49.000 100 45 2 6 104 63 157 6.94e-28 109
MsaG016083 AT1G62700.2 49.000 100 45 2 6 104 63 157 6.94e-28 109
MsaG016083 AT3G10490.1 51.515 99 45 2 6 103 82 178 7.93e-28 106
MsaG016083 AT4G36160.1 51.020 98 42 2 8 104 68 160 2.01e-27 108
MsaG016083 AT4G36160.3 51.020 98 42 2 8 104 68 160 2.01e-27 108
MsaG016083 AT4G36160.2 51.020 98 42 2 8 104 72 164 2.07e-27 108
MsaG016083 AT2G18060.3 48.039 102 47 2 6 106 65 161 2.28e-27 107
MsaG016083 AT2G18060.1 48.039 102 47 2 6 106 65 161 2.28e-27 107
MsaG016083 AT1G71930.2 48.485 99 45 2 6 103 65 158 3.22e-27 106
MsaG016083 AT1G71930.1 48.485 99 45 2 6 103 65 158 3.22e-27 106
MsaG016083 AT3G10490.2 51.515 99 45 2 6 103 82 178 4.75e-27 107
MsaG016083 AT1G34180.4 45.794 107 54 3 1 106 78 181 8.10e-27 107
MsaG016083 AT1G34180.3 46.226 106 53 3 1 105 66 168 8.46e-27 107
MsaG016083 AT1G12260.2 48.515 101 44 3 6 104 63 157 1.41e-26 105
MsaG016083 AT1G12260.1 48.515 101 44 3 6 104 63 157 1.67e-26 105
MsaG016083 AT1G34180.1 46.226 106 53 3 1 105 66 168 1.89e-26 107
MsaG016083 AT1G34180.2 46.226 106 53 3 1 105 78 180 1.90e-26 107
MsaG016083 AT5G46590.2 55.294 85 36 1 6 88 61 145 2.11e-26 103
MsaG016083 AT3G12910.1 49.550 111 43 3 6 103 75 185 2.65e-26 103
MsaG016083 AT5G66300.1 45.045 111 53 3 6 115 68 171 3.76e-26 103
MsaG016083 AT3G49530.1 41.880 117 63 2 6 120 68 181 4.21e-26 105
MsaG016083 AT3G49530.2 41.880 117 63 2 6 120 68 181 4.36e-26 104
MsaG016083 AT3G61910.1 42.017 119 58 3 6 113 67 185 4.69e-26 103
MsaG016083 AT1G34190.1 45.794 107 54 3 1 106 66 169 7.68e-26 105
MsaG016083 AT1G32510.1 48.598 107 43 3 6 103 61 164 1.59e-25 101
MsaG016083 AT1G54330.1 48.113 106 48 2 6 110 61 160 7.55e-25 99.8
MsaG016083 AT5G64060.1 43.137 102 54 3 5 105 60 158 5.33e-24 98.6
MsaG016083 AT2G43000.2 48.485 99 47 3 15 112 2 97 9.92e-24 94.4
MsaG016083 AT5G09330.5 42.157 102 55 2 5 105 60 158 1.83e-23 98.2
MsaG016083 AT5G09330.2 42.157 102 55 2 5 105 60 158 1.83e-23 98.2
MsaG016083 AT5G09330.4 42.157 102 55 2 5 105 60 158 1.83e-23 98.2
MsaG016083 AT5G09330.3 42.157 102 55 2 5 105 60 158 1.83e-23 98.2
MsaG016083 AT5G09330.1 42.157 102 55 2 5 105 60 158 1.83e-23 98.2
MsaG016083 AT3G44350.2 41.803 122 66 4 7 126 64 182 3.72e-23 94.0
MsaG016083 AT5G22380.1 35.233 193 92 9 7 192 65 231 4.48e-23 93.6
MsaG016083 AT5G22290.1 38.889 126 59 5 6 123 75 190 3.51e-22 93.2
MsaG016083 AT1G32870.2 49.020 102 48 3 6 106 65 163 3.66e-22 94.7
MsaG016083 AT1G32870.3 49.020 102 48 3 6 106 101 199 3.75e-22 94.7
MsaG016083 AT1G32870.1 49.020 102 48 3 6 106 65 163 3.82e-22 94.7
MsaG016083 AT3G44290.2 40.541 111 51 3 6 111 68 168 2.22e-21 89.0
MsaG016083 AT2G27300.1 33.721 172 87 6 6 176 68 213 3.20e-21 90.5
MsaG016083 AT3G44290.1 40.179 112 52 3 5 111 67 168 7.62e-21 89.4
MsaG016083 AT3G44350.1 49.333 75 36 1 7 79 64 138 4.36e-20 84.0
MsaG016083 AT3G10490.4 50.633 79 36 2 26 103 2 78 1.80e-18 83.2
MsaG016083 AT3G10490.3 50.633 79 36 2 26 103 2 78 1.80e-18 83.2
MsaG016083 AT4G01540.4 41.237 97 45 2 8 100 63 151 8.74e-18 81.6
MsaG016083 AT4G01540.3 41.237 97 45 2 8 100 63 151 9.14e-18 81.6
MsaG016083 AT4G01540.1 41.237 97 45 2 8 100 63 151 1.09e-17 81.6
MsaG016083 AT4G01540.2 41.237 97 45 2 8 100 63 151 1.22e-17 81.3
MsaG016083 AT4G01520.1 40.206 97 46 2 8 100 63 151 1.66e-17 79.7
MsaG016083 AT4G01550.1 38.384 99 49 2 9 103 63 153 1.04e-15 75.9
MsaG016083 AT5G04395.1 44.578 83 42 2 6 84 94 176 1.81e-15 72.4
MsaG016083 AT2G18060.2 44.156 77 37 2 31 106 2 73 1.96e-15 73.9
MsaG016083 AT3G04420.2 46.479 71 37 1 6 75 60 130 1.02e-14 72.0
MsaG016083 AT3G04420.1 46.479 71 37 1 6 75 60 130 1.75e-14 71.6
MsaG016083 AT4G28530.2 35.922 103 25 1 1 103 74 135 6.71e-13 67.0
MsaG016083 AT5G22290.2 37.000 100 45 5 32 123 2 91 7.43e-13 66.2
MsaG016083 AT1G02230.1 42.254 71 34 2 9 75 63 130 2.72e-11 62.8
MsaG016083 AT1G02220.1 44.118 68 34 2 9 75 63 127 3.06e-11 62.8

Find 76 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCAATGGATTAGTATTT+TGG 0.127740 3:-94015780 None:intergenic
CGCAAACTACGGCTGGAATT+AGG 0.167335 3:+94015569 MsaT016083.1:CDS
TTTCAATGCTCAAAAGGTTT+TGG 0.220852 3:-94015821 None:intergenic
GCCACCCTGAACACTGGTTT+TGG 0.242265 3:+94015754 MsaT016083.1:CDS
TTCTTGATTCCAACCGGTTT+AGG 0.266059 3:-94014771 None:intergenic
GGGCGGCTGGGTCTGGATAT+TGG 0.274315 3:+94014715 MsaT016083.1:CDS
TTTATAGGTATGGCTTCTTA+TGG 0.275721 3:+94014627 None:intergenic
TCAAGGCCGAACCGGGCGGC+TGG 0.280890 3:+94014702 MsaT016083.1:CDS
CCGAACCGGGCGGCTGGGTC+TGG 0.306529 3:+94014708 MsaT016083.1:CDS
TCTTGATTCCAACCGGTTTA+GGG 0.332888 3:-94014770 None:intergenic
GCAAACTACGGCTGGAATTA+GGG 0.333355 3:+94015570 MsaT016083.1:CDS
CGGTTCGGCCTTGAACCGTT+TGG 0.337242 3:-94014693 None:intergenic
TGCCCTTCTTGTTGTAAATA+CGG 0.356578 3:-94015441 None:intergenic
GGAAGCCAAAACCAGTGTTC+AGG 0.356962 3:-94015759 None:intergenic
GGTTCGGCCTTGAACCGTTT+GGG 0.375212 3:-94014692 None:intergenic
GTGGCATCCACGTAATTATA+AGG 0.393502 3:-94015736 None:intergenic
TCTGGATATTGGAAGGCAAC+CGG 0.400133 3:+94014726 MsaT016083.1:CDS
CTCGAATCGGTGTGCAGCTT+AGG 0.410548 3:-94015625 None:intergenic
ACCGGTTTAGGGTGACCTAT+TGG 0.422320 3:-94014759 None:intergenic
CCAGACCCAGCCGCCCGGTT+CGG 0.426418 3:-94014708 None:intergenic
CGTAATTATAAGGAAATTCA+AGG 0.432406 3:-94015726 None:intergenic
CCAGCCGTAGTTTGCGGCGG+AGG 0.437220 3:-94015562 None:intergenic
CAAACGGTTCAAGGCCGAAC+CGG 0.447727 3:+94014694 MsaT016083.1:CDS
CTGGATATTGGAAGGCAACC+GGG 0.449962 3:+94014727 MsaT016083.1:CDS
TTTGTCTTTCAATGCTCAAA+AGG 0.450438 3:-94015827 None:intergenic
CAAGGCCGAACCGGGCGGCT+GGG 0.451424 3:+94014703 MsaT016083.1:CDS
AAAGCTTTCTTGATTCCAAC+CGG 0.462419 3:-94014777 None:intergenic
GATCATGCACGAGTATCGTT+TGG 0.466085 3:+94014845 MsaT016083.1:CDS
CTAAAGGTGATAAAACCAAT+TGG 0.468401 3:+94014823 MsaT016083.1:CDS
ACCTATTGGTTTATCCGCCC+CGG 0.468728 3:-94014745 None:intergenic
GTAGTTTGCGGCGGAGGATG+TGG 0.472297 3:-94015556 None:intergenic
ACCAATAGGTCACCCTAAAC+CGG 0.472837 3:+94014758 MsaT016083.1:CDS
CTAATTCCAGCCGTAGTTTG+CGG 0.474275 3:-94015568 None:intergenic
TATGGCTTCTTATGGAGAAA+AGG 0.474341 3:+94014635 None:intergenic
GTGAGCCTAAGTGGAATGAA+TGG 0.482300 3:+94015695 MsaT016083.1:CDS
GAAGCCAAAACCAGTGTTCA+GGG 0.483454 3:-94015758 None:intergenic
GGTTGGAATCAAGAAAGCTT+TGG 0.483861 3:+94014779 MsaT016083.1:CDS
GAAAGTACCCAAACGGTTCA+AGG 0.487674 3:+94014685 MsaT016083.1:CDS
AGGTGCAGAGTGAGCCTAAG+TGG 0.496331 3:+94015686 MsaT016083.1:CDS
CCTCCGCCGCAAACTACGGC+TGG 0.497456 3:+94015562 MsaT016083.1:CDS
ATACTCGTGCATGATCCAAT+TGG 0.497532 3:-94014838 None:intergenic
TTCTCCCATTCATTCCACTT+AGG 0.500482 3:-94015700 None:intergenic
TTGCCGTATTTACAACAAGA+AGG 0.503330 3:+94015438 MsaT016083.1:CDS
GCACACCGATTCGAGCTGTT+CGG 0.523824 3:+94015633 MsaT016083.1:CDS
CCTCCACGTGTCACGATCTC+CGG 0.524239 3:-94015523 None:intergenic
ACATCCTCCGCCGCAAACTA+CGG 0.532859 3:+94015558 MsaT016083.1:CDS
TTCGGAGCACGTGGTATCAC+CGG 0.534411 3:+94015651 MsaT016083.1:CDS
AAACGGTTCAAGGCCGAACC+GGG 0.545704 3:+94014695 MsaT016083.1:CDS
TGAGCCTAAGTGGAATGAAT+GGG 0.547748 3:+94015696 MsaT016083.1:CDS
ACCGGGGCGGATAAACCAAT+AGG 0.550145 3:+94014744 MsaT016083.1:CDS
TGCACCTCACTTGCAAACTC+CGG 0.557338 3:-94015670 None:intergenic
TACGCGGGTAAAGCACCTAA+AGG 0.558141 3:+94014807 MsaT016083.1:CDS
GAGATCGTGACACGTGGAGG+TGG 0.558979 3:+94015526 MsaT016083.1:CDS
CTTCTTATGGAGAAAAGGAA+TGG 0.562020 3:+94014640 MsaT016083.1:CDS
TTTGGGTACTTTCTGTCTCG+TGG 0.562928 3:-94014675 None:intergenic
GTGCTCCGAACAGCTCGAAT+CGG 0.563123 3:-94015638 None:intergenic
TGCCGTATTTACAACAAGAA+GGG 0.564535 3:+94015439 MsaT016083.1:CDS
ATCACCGGAGTTTGCAAGTG+AGG 0.564595 3:+94015666 MsaT016083.1:CDS
AACATTCTGAAATCGAAGAC+AGG 0.566257 3:+94015497 MsaT016083.1:CDS
GTGGATGCCACCCTGAACAC+TGG 0.570340 3:+94015748 MsaT016083.1:CDS
TGAAATCGAAGACAGGAAGC+CGG 0.575677 3:+94015504 MsaT016083.1:CDS
AATATCCAGACCCAGCCGCC+CGG 0.582754 3:-94014713 None:intergenic
CGAGACAGAAAGTACCCAAA+CGG 0.588955 3:+94014678 MsaT016083.1:CDS
TACATGAACATATCTTGCAA+TGG 0.597049 3:-94015793 None:intergenic
TGGATATTGGAAGGCAACCG+GGG 0.604916 3:+94014728 MsaT016083.1:CDS
ATAGGTCACCCTAAACCGGT+TGG 0.608554 3:+94014762 MsaT016083.1:CDS
GTTTGCGGCGGAGGATGTGG+CGG 0.614306 3:-94015553 None:intergenic
TGAATTTCCTTATAATTACG+TGG 0.635048 3:+94015729 MsaT016083.1:CDS
AAGCCGGAGATCGTGACACG+TGG 0.637296 3:+94015520 MsaT016083.1:CDS
TTCGAGCTGTTCGGAGCACG+TGG 0.644987 3:+94015642 MsaT016083.1:CDS
ATTCCAGCCGTAGTTTGCGG+CGG 0.647744 3:-94015565 None:intergenic
CCGGAGATCGTGACACGTGG+AGG 0.654065 3:+94015523 MsaT016083.1:CDS
GGCTGGGTCTGGATATTGGA+AGG 0.659634 3:+94014719 MsaT016083.1:CDS
CGGTTCAAGGCCGAACCGGG+CGG 0.700064 3:+94014698 MsaT016083.1:CDS
ATATTGGAAGGCAACCGGGG+CGG 0.704098 3:+94014731 MsaT016083.1:CDS
GCCAAAACCAGTGTTCAGGG+TGG 0.722298 3:-94015755 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 94014636 94015834 94014636 ID=MsaG016083
Chr3 mRNA 94014636 94015834 94014636 ID=MsaT016083.1;Parent=MsaG016083
Chr3 exon 94014636 94014908 94014636 ID=MsaT016083.1.exon1;Parent=MsaT016083.1
Chr3 CDS 94014636 94014908 94014636 ID=cds.MsaT016083.1;Parent=MsaT016083.1
Chr3 exon 94015421 94015834 94015421 ID=MsaT016083.1.exon2;Parent=MsaT016083.1
Chr3 CDS 94015421 94015834 94015421 ID=cds.MsaT016083.1;Parent=MsaT016083.1
Gene Sequence

>MsaT016083.1

ATGGCTTCTTATGGAGAAAAGGAATGGTACTTTTTCTCGCCACGAGACAGAAAGTACCCAAACGGTTCAAGGCCGAACCGGGCGGCTGGGTCTGGATATTGGAAGGCAACCGGGGCGGATAAACCAATAGGTCACCCTAAACCGGTTGGAATCAAGAAAGCTTTGGTGTTTTACGCGGGTAAAGCACCTAAAGGTGATAAAACCAATTGGATCATGCACGAGTATCGTTTGGCTGATGTAGATCGCACCATTCGCAAAAAGAACAGCTTAAGGTTGGATGATTGGGTGCTTTGCCGTATTTACAACAAGAAGGGCACAATTGAGAAACAACCAAGCAACCGAAAAACAGAACATTCTGAAATCGAAGACAGGAAGCCGGAGATCGTGACACGTGGAGGTGGTCTTCCGCCACATCCTCCGCCGCAAACTACGGCTGGAATTAGGGATTACATGTACTTTGACACATCAGATTCGATTCCTAAGCTGCACACCGATTCGAGCTGTTCGGAGCACGTGGTATCACCGGAGTTTGCAAGTGAGGTGCAGAGTGAGCCTAAGTGGAATGAATGGGAGAAGAACCTTGAATTTCCTTATAATTACGTGGATGCCACCCTGAACACTGGTTTTGGCTTCCAAAATACTAATCCATTGCAAGATATGTTCATGTACTTGCCCAAAACCTTTTGA
Protein sequence

>MsaT016083.1

MASYGEKEWYFFSPRDRKYPNGSRPNRAAGSGYWKATGADKPIGHPKPVGIKKALVFYAGKAPKGDKTNWIMHEYRLADVDRTIRKKNSLRLDDWVLCRIYNKKGTIEKQPSNRKTEHSEIEDRKPEIVTRGGGLPPHPPPQTTAGIRDYMYFDTSDSIPKLHTDSSCSEHVVSPEFASEVQSEPKWNEWEKNLEFPYNYVDATLNTGFGFQNTNPLQDMFMYLPKTF*