AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016117


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016117 MtrunA17_Chr1g0169171 71.533 274 58 4 34 305 1 256 3.32e-119 348
MsaG016117 MtrunA17_Chr1g0169161 61.564 307 97 3 1 305 1 288 2.93e-105 314
MsaG016117 MtrunA17_Chr3g0125161 88.430 121 14 0 2 122 13 133 1.18e-70 219
MsaG016117 MtrunA17_Chr3g0125151 92.405 79 6 0 227 305 1 79 5.74e-49 162
MsaG016117 MtrunA17_Chr1g0169081 72.222 108 30 0 5 112 2 109 1.24e-43 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 85 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCATCATCTTCAAGTTT+TGG 0.269280 3:+94313434 None:intergenic
GCATAGAAGTATGGATTTCC+TGG 0.326303 3:+94313389 None:intergenic
AGAGAGGAGAAATACGTAAA+TGG 0.332365 3:-94312832 MsaT016117.1:CDS
ACTTTCCCGGCGGTGGAAAC+GGG 0.333345 3:-94311438 MsaT016117.1:CDS
AACTTTCCCGGCGGTGGAAA+CGG 0.347641 3:-94311439 MsaT016117.1:CDS
CAGAGAAGAAAATTCATTAG+AGG 0.353395 3:-94313961 MsaT016117.1:CDS
TGCCATATTGCGTGTCCGCC+TGG 0.359320 3:+94311598 None:intergenic
ATGAGCTTCACAAAAGCAAT+TGG 0.382357 3:+94314104 None:intergenic
AGCTCAGACGGAGGCGGTGG+TGG 0.386990 3:-94311562 MsaT016117.1:CDS
CGCGTCAAAGCACATTCAAT+TGG 0.401347 3:-94314020 MsaT016117.1:CDS
TATGTTGGAGGATGAGGAAG+AGG 0.406388 3:-94313823 MsaT016117.1:CDS
ACTTTCCTAGTGGTGGTAAA+GGG 0.412711 3:-94311528 MsaT016117.1:CDS
GGCCAGGCGGACACGCAATA+TGG 0.417457 3:-94311600 MsaT016117.1:CDS
GTCATAAACTTTCCCGGCGG+TGG 0.417743 3:-94311445 MsaT016117.1:CDS
TAATAATATGTTGGAGGATG+AGG 0.419640 3:-94313829 MsaT016117.1:CDS
ACATTACAGACCCAACGAAT+TGG 0.430909 3:-94313363 MsaT016117.1:intron
AACTTTCCTAGTGGTGGTAA+AGG 0.439112 3:-94311529 MsaT016117.1:CDS
GTGTTGATGGCTATATAGTT+TGG 0.439892 3:-94311621 MsaT016117.1:CDS
GTATGTGTTGTGTTGATTTG+TGG 0.448016 3:+94312860 None:intergenic
GCAAATGTCATAAACTTTCC+CGG 0.460290 3:-94311451 MsaT016117.1:CDS
CCTTGCAGTGGCAACATACA+AGG 0.465539 3:+94313039 None:intergenic
GATTGTGGTTTAGGGAGCAT+TGG 0.466163 3:-94313473 MsaT016117.1:intron
GCCTCCGTCTGAGCTTCCAC+TGG 0.480136 3:+94311570 None:intergenic
AAGAACTAAACCAATTCGTT+GGG 0.487807 3:+94313353 None:intergenic
ATGAAAGCAGAAAAGAAGAA+AGG 0.488071 3:-94313645 MsaT016117.1:CDS
AAAGAACTAAACCAATTCGT+TGG 0.488811 3:+94313352 None:intergenic
CTCTTCCTCTATGATCCCTT+AGG 0.492258 3:+94314048 None:intergenic
TTGTGGCAATATGTGATGAT+AGG 0.496982 3:+94312877 None:intergenic
GGAGGAAAATAATAATATGT+TGG 0.500575 3:-94313838 MsaT016117.1:CDS
TCTCATAATGCTTCTCTATG+AGG 0.504327 3:+94314318 None:intergenic
GGAAGCTCAGACGGAGGCGG+TGG 0.504416 3:-94311565 MsaT016117.1:CDS
GGGATCATAGAGGAAGAGAT+TGG 0.510517 3:-94314043 MsaT016117.1:CDS
CTTTCCCGGCGGTGGAAACG+GGG 0.511813 3:-94311437 MsaT016117.1:CDS
ATCATAAACTTTCCTAGTGG+TGG 0.514983 3:-94311535 MsaT016117.1:CDS
AAGCACATTCAATTGGTGGC+AGG 0.516729 3:-94314013 MsaT016117.1:CDS
AATGGTAGAGGAAGAAGAAG+AGG 0.518113 3:-94313745 MsaT016117.1:CDS
AAGAAGAAAGGAGTTGACAA+GGG 0.522584 3:-94313633 MsaT016117.1:CDS
GAAATACGTAAATGGAAAGA+TGG 0.523151 3:-94312824 MsaT016117.1:intron
AGAGAAGCATTATGAGAGAA+TGG 0.527328 3:-94314312 MsaT016117.1:intron
GGAAAATAATAATATGTTGG+AGG 0.527415 3:-94313835 MsaT016117.1:CDS
GATGGCTATATAGTTTGGCC+AGG 0.530422 3:-94311616 MsaT016117.1:CDS
GAAGAGGAAGAAGATGAAGA+TGG 0.532114 3:-94313729 MsaT016117.1:CDS
GTTGAAATGTATATTCAGCC+AGG 0.532204 3:-94313407 MsaT016117.1:CDS
ACCAGTGGAAGCTCAGACGG+AGG 0.532231 3:-94311571 MsaT016117.1:CDS
TTGGAGGATGAGGAAGAGGA+AGG 0.540476 3:-94313819 MsaT016117.1:CDS
TGAAGACGATGATGAGGATG+AGG 0.541376 3:-94313694 MsaT016117.1:CDS
AAAGAAGAAAGGAGTTGACA+AGG 0.542225 3:-94313634 MsaT016117.1:CDS
GAGGGACATAAGTCAAGCGT+TGG 0.544112 3:-94311394 MsaT016117.1:CDS
TGAGCTTCACAAAAGCAATT+GGG 0.545679 3:+94314105 None:intergenic
GTCAAAGCACATTCAATTGG+TGG 0.546694 3:-94314017 MsaT016117.1:CDS
AGCACCAGTGGAAGCTCAGA+CGG 0.551410 3:-94311574 MsaT016117.1:CDS
AAAGACAAAGGTAATGGATG+AGG 0.553323 3:-94313859 MsaT016117.1:CDS
TTTCAATATGTGTGAAAACA+AGG 0.554136 3:+94313928 None:intergenic
GACAAAGGTAATGGATGAGG+AGG 0.556485 3:-94313856 MsaT016117.1:CDS
GAGGAACACCTTGGCAGTGT+TGG 0.557953 3:+94314358 None:intergenic
ATTTGTAGTTGGTGAGACAC+AGG 0.559962 3:-94311662 MsaT016117.1:intron
AGCTCATGCTTTCGAAACAA+AGG 0.561664 3:-94314088 MsaT016117.1:CDS
GGAAGACTTTGAAGAAGTTG+AGG 0.564880 3:+94314339 None:intergenic
GCTCATGCTTTCGAAACAAA+GGG 0.566004 3:-94314087 MsaT016117.1:CDS
CCTTGTATGTTGCCACTGCA+AGG 0.576526 3:-94313039 MsaT016117.1:intron
AGTGGAAGCTCAGACGGAGG+CGG 0.576659 3:-94311568 MsaT016117.1:CDS
CTTTCCTAGTGGTGGTAAAG+GGG 0.585504 3:-94311527 MsaT016117.1:CDS
GATGATGATGATGGTGATGG+TGG 0.586203 3:-94313795 MsaT016117.1:CDS
TTTCCTAGTGGTGGTAAAGG+GGG 0.588254 3:-94311526 MsaT016117.1:CDS
GGCTATATAGTTTGGCCAGG+CGG 0.594243 3:-94311613 MsaT016117.1:CDS
GAAGAAGTTGAGGAACACCT+TGG 0.598409 3:+94314349 None:intergenic
CGACGGTTATACAATGGTAG+AGG 0.603192 3:-94313757 MsaT016117.1:CDS
TTTCCCGGCGGTGGAAACGG+GGG 0.606258 3:-94311436 MsaT016117.1:CDS
TGATATGCTTTGTAAAGCAA+AGG 0.607441 3:+94313063 None:intergenic
TTTATCCTAAGGGATCATAG+AGG 0.616153 3:-94314053 MsaT016117.1:CDS
AATTATGACCAACACTGCCA+AGG 0.625272 3:-94314366 None:intergenic
TGATGATGAAGACGATGATG+AGG 0.632639 3:-94313700 MsaT016117.1:CDS
GCAATATGGCAAAGCACCAG+TGG 0.640117 3:-94311586 MsaT016117.1:CDS
GAAGAAGATGAAGATGGTGA+TGG 0.640201 3:-94313723 MsaT016117.1:CDS
GAGGAAGGTGATGATGATGA+TGG 0.640863 3:-94313804 MsaT016117.1:CDS
ATAACAATTATACCTTGCAG+TGG 0.648271 3:+94313027 None:intergenic
CCCGGAAGTGGAAAACGGGG+TGG 0.655848 3:-94311478 MsaT016117.1:CDS
GGTGATGATGATGATGGTGA+TGG 0.656321 3:-94313798 MsaT016117.1:CDS
ACAACACATACCGAAAAGAG+AGG 0.656927 3:-94312848 MsaT016117.1:CDS
ATTATCATAAACTTTCCTAG+TGG 0.664481 3:-94311538 MsaT016117.1:CDS
CTTGCAGTGGCAACATACAA+GGG 0.670891 3:+94313040 None:intergenic
TTTCCCGGAAGTGGAAAACG+GGG 0.695053 3:-94311481 MsaT016117.1:CDS
TGACGACGACGGTTATACAA+TGG 0.701620 3:-94313763 MsaT016117.1:CDS
GGAGATGATGATGACGACGA+CGG 0.751131 3:-94313774 MsaT016117.1:CDS
AATGTCATAAACTTTCCCGG+CGG 0.795660 3:-94311448 MsaT016117.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 94311372 94314384 94311372 ID=MsaG016117
Chr3 mRNA 94311372 94314384 94311372 ID=MsaT016117.1;Parent=MsaG016117
Chr3 exon 94311372 94311676 94311372 ID=MsaT016117.1.exon6;Parent=MsaT016117.1
Chr3 CDS 94311372 94311676 94311372 ID=cds.MsaT016117.1;Parent=MsaT016117.1
Chr3 exon 94312825 94312924 94312825 ID=MsaT016117.1.exon5;Parent=MsaT016117.1
Chr3 CDS 94312825 94312924 94312825 ID=cds.MsaT016117.1;Parent=MsaT016117.1
Chr3 exon 94313040 94313117 94313040 ID=MsaT016117.1.exon4;Parent=MsaT016117.1
Chr3 CDS 94313040 94313117 94313040 ID=cds.MsaT016117.1;Parent=MsaT016117.1
Chr3 exon 94313364 94313482 94313364 ID=MsaT016117.1.exon3;Parent=MsaT016117.1
Chr3 CDS 94313364 94313482 94313364 ID=cds.MsaT016117.1;Parent=MsaT016117.1
Chr3 exon 94313586 94314132 94313586 ID=MsaT016117.1.exon2;Parent=MsaT016117.1
Chr3 CDS 94313586 94314132 94313586 ID=cds.MsaT016117.1;Parent=MsaT016117.1
Chr3 exon 94314313 94314384 94314313 ID=MsaT016117.1.exon1;Parent=MsaT016117.1
Chr3 CDS 94314313 94314384 94314313 ID=cds.MsaT016117.1;Parent=MsaT016117.1
Gene Sequence

>MsaT016117.1

ATGACCAACACTGCCAAGGTGTTCCTCAACTTCTTCAAAGTCTTCCTCATAGAGAAGCATTATGAGAGAATGCTTATCCCAATTGCTTTTGTGAAGCTCATGCTTTCGAAACAAAGGGTATTGCAATATTTTATCCTAAGGGATCATAGAGGAAGAGATTGGCGCGTCAAAGCACATTCAATTGGTGGCAGGCTTTATTTTGATGATGGTTGGAAACTATTCAGAGAAGAAAATTCATTAGAGGACAGTGACTTCCTTGTTTTCACACATATTGAAAACAATGTATTCAAGTTCAAAATTCTTGAACTATCATCAATGTGTAAAAAGACAAAGGTAATGGATGAGGAGGAAAATAATAATATGTTGGAGGATGAGGAAGAGGAAGGTGATGATGATGATGGTGATGGTGGAGATGATGATGACGACGACGGTTATACAATGGTAGAGGAAGAAGAAGAGGAAGAAGATGAAGATGGTGATGGTGATGATGAAGACGATGATGAGGATGAGGATGCTGATGATGATGTGCATATAATAATGAAAGCAGAAAAGAAGAAAGGAGTTGACAAGGGAATCAGTAGAAGCTGTGAACATCAACATTGTAGAACCTTCAAAGCATGGAGCATTGGAAGCAGCTCTGCAGCTCCAAAACTTGAAGATGATGAAATTGATGTTGAAATGTATATTCAGCCAGGAAATCCATACTTCTATGCAAAACATTACAGACCCAACGAATTGTATATTTTAAAGAATGTTGTTAAGGATTTCTGCCTTTGCTTTACAAAGCATATCACCCTTGTATGTTGCCACTGCAAGGATGTTCGAAGTAATGAAAGAGCAGCCTATCATCACATATTGCCACAAATCAACACAACACATACCGAAAAGAGAGGAGAAATACGTAAATGGAAAGATGTTGGTGAGACACAGGAATCAAAGATAAAAATTGGTGTTGATGGCTATATAGTTTGGCCAGGCGGACACGCAATATGGCAAAGCACCAGTGGAAGCTCAGACGGAGGCGGTGGTGGAATTATCATAAACTTTCCTAGTGGTGGTAAAGGGGGTGGAGGTGGAAACATCATAATTTTTCCCGGAAGTGGAAAACGGGGTGGTCGTGCAAATGTCATAAACTTTCCCGGCGGTGGAAACGGGGGAGGCGGAGGAAAAATAAATGAGGGACATAAGTCAAGCGTTGGAAAAGCCTTCATAAAAACCTAA

Protein sequence

>MsaT016117.1

MTNTAKVFLNFFKVFLIEKHYERMLIPIAFVKLMLSKQRVLQYFILRDHRGRDWRVKAHSIGGRLYFDDGWKLFREENSLEDSDFLVFTHIENNVFKFKILELSSMCKKTKVMDEEENNNMLEDEEEEGDDDDGDGGDDDDDDGYTMVEEEEEEEDEDGDGDDEDDDEDEDADDDVHIIMKAEKKKGVDKGISRSCEHQHCRTFKAWSIGSSSAAPKLEDDEIDVEMYIQPGNPYFYAKHYRPNELYILKNVVKDFCLCFTKHITLVCCHCKDVRSNERAAYHHILPQINTTHTEKRGEIRKWKDVGETQESKIKIGVDGYIVWPGGHAIWQSTSGSSDGGGGGIIINFPSGGKGGGGGNIIIFPGSGKRGGRANVINFPGGGNGGGGGKINEGHKSSVGKAFIKT*