Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016157 | XP_039688021.1 | 69.744 | 704 | 196 | 9 | 22 | 718 | 722 | 1415 | 0.0 | 981 |
| MsaG016157 | XP_039688021.1 | 79.862 | 725 | 136 | 6 | 1 | 723 | 1 | 717 | 0.0 | 1169 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016157 | sp|Q9LMN8|WAK3_ARATH | 44.832 | 745 | 347 | 24 | 3 | 719 | 1 | 709 | 0.0 | 575 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016157 | A0A072V215 | 74.967 | 763 | 171 | 9 | 1 | 749 | 1 | 757 | 0.0 | 1162 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016157 | MtrunA17_Chr3g0125431 | 74.967 | 763 | 171 | 9 | 1 | 749 | 1 | 757 | 0.0 | 1162 |
| MsaG016157 | MtrunA17_Chr2g0279661 | 72.200 | 759 | 198 | 8 | 1 | 749 | 3 | 758 | 0.0 | 1092 |
| MsaG016157 | MtrunA17_Chr3g0125441 | 69.330 | 776 | 201 | 8 | 5 | 749 | 18 | 787 | 0.0 | 1085 |
| MsaG016157 | MtrunA17_Chr3g0125451 | 71.260 | 762 | 192 | 10 | 3 | 749 | 2 | 751 | 0.0 | 1067 |
| MsaG016157 | MtrunA17_Chr3g0125461 | 72.599 | 708 | 179 | 5 | 1 | 702 | 23 | 721 | 0.0 | 1043 |
| MsaG016157 | MtrunA17_Chr3g0125471 | 70.676 | 740 | 142 | 8 | 1 | 738 | 1 | 667 | 0.0 | 1005 |
| MsaG016157 | MtrunA17_Chr1g0158201 | 46.291 | 728 | 340 | 16 | 9 | 719 | 14 | 707 | 0.0 | 607 |
| MsaG016157 | MtrunA17_Chr1g0158261 | 46.612 | 738 | 346 | 19 | 9 | 733 | 15 | 717 | 0.0 | 605 |
| MsaG016157 | MtrunA17_Chr1g0158251 | 45.418 | 742 | 364 | 17 | 9 | 737 | 15 | 728 | 0.0 | 605 |
| MsaG016157 | MtrunA17_Chr1g0148501 | 45.280 | 731 | 363 | 16 | 10 | 720 | 13 | 726 | 0.0 | 600 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016157 | AT1G21240.1 | 44.832 | 745 | 347 | 24 | 3 | 719 | 1 | 709 | 0.0 | 575 |
| MsaG016157 | AT1G21270.1 | 42.970 | 761 | 379 | 22 | 9 | 749 | 7 | 732 | 0.0 | 565 |
| MsaG016157 | AT1G21230.1 | 43.533 | 719 | 364 | 19 | 9 | 713 | 7 | 697 | 0.0 | 561 |
| MsaG016157 | AT1G21250.1 | 44.154 | 727 | 357 | 19 | 12 | 719 | 7 | 703 | 0.0 | 556 |
| MsaG016157 | AT1G21210.1 | 40.828 | 725 | 383 | 16 | 8 | 714 | 4 | 700 | 1.42e-175 | 521 |
| MsaG016157 | AT1G16130.1 | 41.737 | 714 | 359 | 19 | 10 | 700 | 13 | 692 | 1.17e-162 | 488 |
| MsaG016157 | AT1G79670.1 | 41.241 | 725 | 356 | 23 | 9 | 702 | 14 | 699 | 9.64e-158 | 476 |
| MsaG016157 | AT1G16150.1 | 39.835 | 728 | 361 | 21 | 10 | 700 | 13 | 700 | 1.67e-156 | 473 |
| MsaG016157 | AT1G16120.1 | 40.671 | 745 | 384 | 21 | 8 | 720 | 9 | 727 | 2.00e-152 | 461 |
| MsaG016157 | AT1G79670.2 | 40.361 | 721 | 331 | 19 | 9 | 702 | 14 | 662 | 5.57e-151 | 457 |
Find 178 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAGTTGCTTCTCATGG+TGG | 0.595893 | 3:+94760343 | MsaT016157.1:CDS |
| AAAGATCCTTCTGAGCTTGC+TGG | 0.316429 | 3:-94761072 | None:intergenic |
| AAAGCTTTACCACATTCCGA+TGG | 0.621964 | 3:-94762690 | None:intergenic |
| AAAGGAAACTGCTGGAGTCC+TGG | 0.471596 | 3:+94762843 | MsaT016157.1:CDS |
| AAAGGAAATACATCCACCAC+TGG | 0.586799 | 3:+94760767 | MsaT016157.1:CDS |
| AACAGCTAGCGAAGCATGGA+GGG | 0.579900 | 3:+94762452 | MsaT016157.1:CDS |
| AACCAGACTGAGAATAGCAA+AGG | 0.621122 | 3:+94762825 | MsaT016157.1:CDS |
| AACCATGAATGAAGTGGCAA+TGG | 0.531497 | 3:+94763314 | MsaT016157.1:CDS |
| AACGTGCCAGCAAGCTCAGA+AGG | 0.562874 | 3:+94761066 | MsaT016157.1:CDS |
| AAGACCAACCATGAATGAAG+TGG | 0.628866 | 3:+94763308 | MsaT016157.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 94760326 | 94763509 | 94760326 | ID=MsaG016157 |
| Chr3 | mRNA | 94760326 | 94763509 | 94760326 | ID=MsaT016157.1;Parent=MsaG016157 |
| Chr3 | exon | 94760326 | 94761220 | 94760326 | ID=MsaT016157.1.exon1;Parent=MsaT016157.1 |
| Chr3 | CDS | 94760326 | 94761220 | 94760326 | ID=cds.MsaT016157.1;Parent=MsaT016157.1 |
| Chr3 | exon | 94761676 | 94761846 | 94761676 | ID=MsaT016157.1.exon2;Parent=MsaT016157.1 |
| Chr3 | CDS | 94761676 | 94761846 | 94761676 | ID=cds.MsaT016157.1;Parent=MsaT016157.1 |
| Chr3 | exon | 94762326 | 94763509 | 94762326 | ID=MsaT016157.1.exon3;Parent=MsaT016157.1 |
| Chr3 | CDS | 94762326 | 94763509 | 94762326 | ID=cds.MsaT016157.1;Parent=MsaT016157.1 |
| Gene Sequence |
>MsaT016157.1 ATGGCTGTGGAGTATTCAAAACAGTTGCTTCTCATGGTGGTTGTTGCTCTCCTTTATACAAAAGCAGCAACTCAGCCGATGTCACTACCAAACTGTACAGCAAAGTGTGGTTCCATCAAAATTCCCTTCCCATTTGGAATCACCAAAGATTGCTCCCTAGACAACACTTTCCTCATCAATTGCAAGAAAATATCTTCAACTACATCAAATTCAACAACACACATTCCTTTCTTGCCAAAAACTAATCAAAGGGTTCTAAATATCTCACTCAACGGTGAACTGCACGTTGGATGGCCAGTAGCCAGTGATTGCTATGCAAAAAATAACAAACCATTGAGGAAAAGATTTCAGGATATGACACATTTTCACATCTCGCCGACTCAAAACAAGTTGGTAGCAGTTGGCTGTGGGACAATTGGATATCTTGTAGCAATTGATTCTAAAGGAAATACATCCACCACTGGATGTGTGGCTTTGTGCAACAGCATTAGTGATATTGTGGCCAATGAATCTTGTTCCGGCTCGGGTTGTTGTGAGATTTCAATACCCAAAGGTCACGTGTTTACAGAAGTAACCTATATTTCTGAAGTCCGAGTGAATGATCACCTAGTATATGACTTCAATCCATGTGGTTATGCGTTTTTGGTGGAAAATGGAGCCTACAGCTTTGCAATCACAGACCTCCGCAAGTTGAAGAAGAAAGAATTTCCTGTGTTGTTGGACTGGACAGTAGGGAACAAAACGTGCCAGCAAGCTCAGAAGGATCTTTCAAGTAATTATGCATGTAAGGCAGACAAAAGTATATGTTACGATGCAGCAACCAACAAATCTGGTTACCTTTGCAGATGCTTGGATGGATATCGGGGAAATCCTTACCTCATCCATGGTTGCCAAGATGTTGATGAATGCAAGGAATCCAATTACTGTTTTAAGGGGGCGACATGCAACAATCTTCCTGGAAGCTACCATTGTTTCTGCCCAGAAGGATATGAAGGAGACGGAAAAAGATGCAGTCCAAAATCCTGTACCAAACCAAGGAAAGAGTTCATATTGGTTATTGCATTGAGTGTCAGCGTAAGCCTCCTAGGAGTACTTGTGGGAAGCTTTTATGCGCATTGGGCATTGAGGAAAATAAAGCTCAATAAACGTAAAGAAAATTTTTTTCGACAAAATGGTGGCTTAGTGTTACAACAACAGCTAGCGAAGCATGGAGGGTCAACTGAAATAGTTAAAATCTTCACTATTGAGGAGTTAAATAAGGCAACCAACAACTTCGATGAAGGAAAGATCCTAGGCGAAGGTGGTCAAGGAACAGTTTACAAAGGAATGTTACAAGATGAAAGAGTTGTAGCGATAAAGAAGTCCAAAATCAATGACCCAAACCAGATTGAGCCATTCATCAATGAAGTGATTGTGCTTTCCCAGATCAACCATCGGAATGTGGTAAAGCTTTTGGGATGTTGTTTAGAGATAGAAGTTCCCTTACTTGTTTATGAATTTATTCCCAATGGTACTGTTTATGATCATCTTCATCATCAAAACCAATCTTTAAAGCTTGCATGGAAAACCAGACTGAGAATAGCAAAGGAAACTGCTGGAGTCCTGGCATACTTACATTCCGCTGCTTCTACACCAATCATACATAGAGATGTGAAATCTACAAATATACTCCTAGATCACAATCTCACTGCAAAGGTTTCTGACTTTGGAGCTTCAAAGATTGTTCCTCTTGATCATAGTCAGATAACCACTCTAGTGAAGGGGACATTGGGGTATCTTGATCCTGAATACTTCCACACAAGCCAATTAACAGAGAAGAGTGATGTTTATAGCTTTGGGGTTGTCCTAGCAAAGCTACTGACAGGAAAGAAAGCGCTATCTGTTGGCAGGCCTGAGGTTGATAGAAATCTTGCAATGCACTTTGTTTCTTCAGTGAAAGAAGGTCGGTTACTTCATATTCTGGACAACAATATTTCTGAGGAAAATATTGAGCATCTAAAGGAAGTTTCCCTAATTGCAGAATGGTGTTTAAGGGTGAAGAGCGAGGAAAGACCAACCATGAATGAAGTGGCAATGGGACTAGAGGGAATATTAGCTACTGAAAAGCAGCGTTTGGGAGGTGACAATCTGTCTTCAGAAGAGGCACCATCCATTATCAATGTTGGAGATTGTGTTAGAGAAAATGGCATTAATTCGTCTGACACCTATAGCACGAACCAGATTACGATGTCAACGATTGGTGGTAGATGA |
| Protein sequence |
>MsaT016157.1 MAVEYSKQLLLMVVVALLYTKAATQPMSLPNCTAKCGSIKIPFPFGITKDCSLDNTFLINCKKISSTTSNSTTHIPFLPKTNQRVLNISLNGELHVGWPVASDCYAKNNKPLRKRFQDMTHFHISPTQNKLVAVGCGTIGYLVAIDSKGNTSTTGCVALCNSISDIVANESCSGSGCCEISIPKGHVFTEVTYISEVRVNDHLVYDFNPCGYAFLVENGAYSFAITDLRKLKKKEFPVLLDWTVGNKTCQQAQKDLSSNYACKADKSICYDAATNKSGYLCRCLDGYRGNPYLIHGCQDVDECKESNYCFKGATCNNLPGSYHCFCPEGYEGDGKRCSPKSCTKPRKEFILVIALSVSVSLLGVLVGSFYAHWALRKIKLNKRKENFFRQNGGLVLQQQLAKHGGSTEIVKIFTIEELNKATNNFDEGKILGEGGQGTVYKGMLQDERVVAIKKSKINDPNQIEPFINEVIVLSQINHRNVVKLLGCCLEIEVPLLVYEFIPNGTVYDHLHHQNQSLKLAWKTRLRIAKETAGVLAYLHSAASTPIIHRDVKSTNILLDHNLTAKVSDFGASKIVPLDHSQITTLVKGTLGYLDPEYFHTSQLTEKSDVYSFGVVLAKLLTGKKALSVGRPEVDRNLAMHFVSSVKEGRLLHILDNNISEENIEHLKEVSLIAEWCLRVKSEERPTMNEVAMGLEGILATEKQRLGGDNLSSEEAPSIINVGDCVRENGINSSDTYSTNQITMSTIGGR* |
