AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016216

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016216 MtrunA17_Chr3g0126201 100.000 81 0 0 1 81 29 109 7.27e-58 180
MsaG016216 MtrunA17_Chr1g0177251 87.879 66 7 1 13 78 3 67 6.77e-38 127
MsaG016216 MtrunA17_Chr2g0326001 76.056 71 17 0 7 77 24 94 2.61e-33 115
MsaG016216 MtrunA17_Chr3g0127201 74.324 74 17 1 4 77 10 81 7.00e-33 114
MsaG016216 MtrunA17_Chr4g0030051 74.648 71 18 0 5 75 23 93 9.72e-31 109
MsaG016216 MtrunA17_Chr8g0346551 76.562 64 13 1 14 77 17 78 2.05e-30 107
MsaG016216 MtrunA17_Chr4g0047001 85.455 55 8 0 25 79 57 111 3.04e-30 109
MsaG016216 MtrunA17_Chr2g0328021 62.667 75 28 0 5 79 44 118 7.45e-30 107
MsaG016216 MtrunA17_Chr7g0237921 64.865 74 26 0 4 77 57 130 1.24e-29 107
MsaG016216 MtrunA17_Chr4g0010151 64.865 74 26 0 4 77 63 136 1.66e-29 107
MsaG016216 MtrunA17_Chr1g0188851 58.140 86 28 1 2 79 14 99 2.95e-29 105
MsaG016216 MtrunA17_Chr8g0374251 84.615 52 8 0 25 76 70 121 3.81e-29 105
MsaG016216 MtrunA17_Chr8g0367161 66.667 69 23 0 11 79 20 88 4.60e-29 105
MsaG016216 MtrunA17_Chr7g0223311 76.190 63 12 1 22 81 76 138 5.69e-29 105
MsaG016216 MtrunA17_Chr4g0031411 63.514 74 27 0 4 77 56 129 5.87e-29 105
MsaG016216 MtrunA17_Chr3g0117601 73.333 60 16 0 14 73 52 111 1.82e-28 103
MsaG016216 MtrunA17_Chr4g0061761 87.755 49 6 0 25 73 42 90 2.55e-28 103
MsaG016216 MtrunA17_Chr2g0282441 86.000 50 7 0 25 74 27 76 1.56e-27 101
MsaG016216 MtrunA17_Chr5g0411951 85.714 49 7 0 25 73 45 93 4.65e-27 100
MsaG016216 MtrunA17_Chr8g0341671 82.000 50 9 0 25 74 22 71 9.34e-27 97.4
MsaG016216 MtrunA17_Chr2g0308011 82.353 51 9 0 21 71 35 85 2.30e-26 96.3
MsaG016216 MtrunA17_Chr2g0282051 70.968 62 17 1 18 79 41 101 3.24e-26 97.1
MsaG016216 MtrunA17_Chr8g0374111 70.492 61 17 1 17 77 24 83 4.03e-26 95.9
MsaG016216 MtrunA17_Chr2g0282511 84.000 50 8 0 22 71 44 93 4.70e-26 97.4
MsaG016216 MtrunA17_Chr4g0047761 76.786 56 12 1 24 79 73 127 6.60e-26 97.1
MsaG016216 MtrunA17_Chr3g0092111 64.407 59 21 0 25 83 138 196 6.61e-25 95.9
MsaG016216 MtrunA17_Chr7g0217581 69.643 56 16 1 21 76 231 285 6.59e-24 93.6
MsaG016216 MtrunA17_Chr6g0453321 71.154 52 15 0 25 76 138 189 2.09e-23 92.0
MsaG016216 MtrunA17_Chr2g0283811 65.517 58 20 0 19 76 43 100 2.50e-23 87.0
MsaG016216 MtrunA17_Chr4g0042481 59.155 71 27 2 6 76 107 175 3.83e-23 90.9
MsaG016216 MtrunA17_Chr5g0417571 56.338 71 29 2 6 76 123 191 4.28e-23 89.7
MsaG016216 MtrunA17_Chr7g0254161 66.038 53 18 0 25 77 103 155 1.61e-22 89.0
MsaG016216 MtrunA17_Chr8g0355261 62.963 54 20 0 25 78 120 173 1.78e-22 89.0
MsaG016216 MtrunA17_Chr6g0460731 52.000 75 34 1 3 77 78 150 4.28e-22 87.4
MsaG016216 MtrunA17_Chr6g0460721 52.000 75 34 1 3 77 71 143 5.35e-22 87.0
MsaG016216 MtrunA17_Chr5g0417601 54.667 75 32 2 6 80 125 197 5.79e-22 87.4
MsaG016216 MtrunA17_Chr5g0417561 55.714 70 24 1 7 76 110 172 6.78e-21 84.3
MsaG016216 MtrunA17_Chr5g0417551 62.712 59 18 1 22 76 122 180 1.27e-20 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016216 AT3G52440.1 80.000 70 10 1 15 80 13 82 6.70e-36 121
MsaG016216 AT3G52440.2 80.000 70 10 1 15 80 32 101 8.26e-36 122
MsaG016216 AT1G21340.1 72.840 81 17 2 3 79 15 94 3.95e-35 120
MsaG016216 AT5G60200.1 82.759 58 10 0 20 77 52 109 2.27e-32 113
MsaG016216 AT4G24060.1 78.125 64 14 0 14 77 44 107 3.03e-32 114
MsaG016216 AT2G28510.1 80.645 62 12 0 22 83 47 108 7.78e-32 112
MsaG016216 AT3G61850.2 76.562 64 13 1 14 77 55 116 6.48e-31 109
MsaG016216 AT3G61850.3 76.562 64 13 1 14 77 55 116 6.48e-31 109
MsaG016216 AT3G61850.1 76.562 64 13 1 14 77 67 128 9.19e-31 109
MsaG016216 AT3G61850.5 76.562 64 13 1 14 77 55 116 1.13e-30 109
MsaG016216 AT3G61850.4 87.037 54 7 0 24 77 75 128 1.45e-30 109
MsaG016216 AT2G37590.1 78.333 60 13 0 20 79 86 145 2.78e-30 109
MsaG016216 AT3G55370.1 75.000 64 16 0 17 80 70 133 2.97e-30 108
MsaG016216 AT3G55370.2 75.000 64 16 0 17 80 70 133 3.24e-30 109
MsaG016216 AT3G55370.3 75.000 64 16 0 17 80 115 178 3.39e-30 109
MsaG016216 AT1G07640.2 82.143 56 9 1 25 79 79 134 1.66e-29 107
MsaG016216 AT1G07640.1 82.143 56 9 1 25 79 23 78 2.18e-29 105
MsaG016216 AT1G07640.3 82.143 56 9 1 25 79 87 142 2.21e-29 107
MsaG016216 AT4G00940.1 85.185 54 8 0 24 77 69 122 3.33e-29 105
MsaG016216 AT4G00940.3 85.185 54 8 0 24 77 69 122 3.33e-29 105
MsaG016216 AT4G00940.2 85.185 54 8 0 24 77 69 122 3.33e-29 105
MsaG016216 AT5G60850.1 82.143 56 10 0 25 80 53 108 1.19e-28 104
MsaG016216 AT5G02460.1 73.770 61 16 0 17 77 89 149 1.37e-28 105
MsaG016216 AT3G45610.1 70.968 62 18 0 20 81 37 98 3.35e-28 102
MsaG016216 AT5G65590.1 81.132 53 10 0 25 77 43 95 4.16e-28 103
MsaG016216 AT1G28310.1 81.818 55 10 0 25 79 29 83 4.26e-28 103
MsaG016216 AT1G64620.1 67.606 71 20 1 2 72 31 98 5.22e-28 103
MsaG016216 AT1G28310.2 81.818 55 10 0 25 79 53 107 5.33e-28 103
MsaG016216 AT4G21050.1 73.333 60 16 0 25 84 26 85 1.08e-27 99.8
MsaG016216 AT2G28810.2 76.364 55 13 0 20 74 74 128 1.16e-27 102
MsaG016216 AT3G21270.1 86.275 51 7 0 25 75 31 81 1.17e-27 99.8
MsaG016216 AT2G46590.1 91.489 47 4 0 24 70 69 115 1.24e-27 102
MsaG016216 AT1G51700.1 79.245 53 11 0 25 77 35 87 1.30e-27 99.4
MsaG016216 AT2G46590.2 91.489 47 4 0 24 70 81 127 1.49e-27 102
MsaG016216 AT5G62940.1 87.755 49 6 0 25 73 75 123 1.63e-27 102
MsaG016216 AT2G28810.1 76.364 55 13 0 20 74 91 145 1.85e-27 102
MsaG016216 AT3G50410.1 77.358 53 12 0 25 77 32 84 4.26e-26 96.7
MsaG016216 AT4G38000.1 65.079 63 22 0 11 73 29 91 2.38e-25 94.7
MsaG016216 AT5G66940.1 70.370 54 16 0 24 77 33 86 1.05e-24 92.4
MsaG016216 AT1G69570.1 64.706 68 22 1 9 76 120 185 1.10e-24 95.1
MsaG016216 AT1G29160.1 71.154 52 15 0 25 76 64 115 5.59e-24 89.4
MsaG016216 AT2G34140.1 71.154 52 15 0 25 76 60 111 5.90e-24 89.4
MsaG016216 AT3G47500.1 71.154 52 15 0 25 76 112 163 1.15e-23 92.8
MsaG016216 AT4G21040.1 75.000 52 13 0 25 76 27 78 1.25e-23 89.7
MsaG016216 AT1G47655.1 69.091 55 17 0 17 71 23 77 1.52e-23 89.0
MsaG016216 AT1G26790.1 57.746 71 29 1 6 76 95 164 3.06e-23 90.9
MsaG016216 AT5G62430.1 54.930 71 31 1 6 76 38 107 6.18e-23 89.4
MsaG016216 AT5G39660.3 71.154 52 15 0 25 76 140 191 1.32e-22 90.1
MsaG016216 AT5G39660.1 71.154 52 15 0 25 76 140 191 1.32e-22 90.1
MsaG016216 AT5G39660.2 71.154 52 15 0 25 76 140 191 1.32e-22 90.1
MsaG016216 AT4G21080.1 71.154 52 15 0 25 76 27 78 3.10e-22 86.7
MsaG016216 AT4G21030.1 62.000 50 19 0 25 74 23 72 2.99e-15 67.4

Find 24 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTGTAGTAACAGAATT+TGG 0.230505 3:+95419828 None:intergenic
GCAAAGGTTGTAGACGTTAT+TGG 0.239568 3:-95419777 MsaT016216.1:CDS
GAAGCACAACGAGGACAATT+AGG 0.359511 3:+95419859 None:intergenic
ATTTGTCCATTGAGGTTGTT+GGG 0.398492 3:+95419917 None:intergenic
TCCATTGAGGTTGTTGGGAA+TGG 0.426025 3:+95419922 None:intergenic
TTGCGGCAGCCACCGCCAAC+GGG 0.427583 3:+95419727 None:intergenic
GGACCAAAGGTGGCTCTCTT+CGG 0.446025 3:-95419756 MsaT016216.1:CDS
CTTCGGAATGTTCCCGTTGG+CGG 0.470284 3:-95419739 MsaT016216.1:CDS
CGGAATGTTCCCGTTGGCGG+TGG 0.477735 3:-95419736 MsaT016216.1:CDS
TTTGCGGCAGCCACCGCCAA+CGG 0.485880 3:+95419726 None:intergenic
ATTCCGAAGAGAGCCACCTT+TGG 0.486871 3:+95419753 None:intergenic
GCCATTCCCAACAACCTCAA+TGG 0.494342 3:-95419923 MsaT016216.1:CDS
TGAATTTGATTTGTCCATTG+AGG 0.513430 3:+95419909 None:intergenic
GATTTGTCCATTGAGGTTGT+TGG 0.532932 3:+95419916 None:intergenic
TCTCTTCGGAATGTTCCCGT+TGG 0.557776 3:-95419742 MsaT016216.1:CDS
CAACCACGGTATTTCTGCAA+AGG 0.575852 3:-95419793 MsaT016216.1:CDS
TGTAGACGTTATTGGACCAA+AGG 0.588786 3:-95419769 MsaT016216.1:CDS
TGGACAAATCAAATTCAAAG+TGG 0.607924 3:-95419903 MsaT016216.1:CDS
AGACGTTATTGGACCAAAGG+TGG 0.608980 3:-95419766 MsaT016216.1:CDS
TTAGGAGCTATCTCAATGTG+TGG 0.628107 3:+95419877 None:intergenic
ATTCGACCGCCTTTCCACGA+CGG 0.650458 3:+95419701 None:intergenic
CAACCTTTGCAGAAATACCG+TGG 0.687599 3:+95419790 None:intergenic
ATTATAGCTTGTCACAACCA+CGG 0.706519 3:-95419807 MsaT016216.1:CDS
GTGTTTGATGAAGCACAACG+AGG 0.730421 3:+95419850 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 95419693 95419947 95419693 ID=MsaG016216
Chr3 mRNA 95419693 95419947 95419693 ID=MsaT016216.1;Parent=MsaG016216
Chr3 exon 95419693 95419947 95419693 ID=MsaT016216.1.exon1;Parent=MsaT016216.1
Chr3 CDS 95419693 95419947 95419693 ID=cds.MsaT016216.1;Parent=MsaT016216.1
Gene Sequence

>MsaT016216.1

ATGCCATTCCCAACAACCTCAATGGACAAATCAAATTCAAAGTGGAAACCACACATTGAGATAGCTCCTAATTGTCCTCGTTGTGCTTCATCAAACACCAAATTCTGTTACTACAATAATTATAGCTTGTCACAACCACGGTATTTCTGCAAAGGTTGTAGACGTTATTGGACCAAAGGTGGCTCTCTTCGGAATGTTCCCGTTGGCGGTGGCTGCCGCAAAAACCGTCGTGGAAAGGCGGTCGAATTCTCGTGA
Protein sequence

>MsaT016216.1

MPFPTTSMDKSNSKWKPHIEIAPNCPRCASSNTKFCYYNNYSLSQPRYFCKGCRRYWTKGGSLRNVPVGGGCRKNRRGKAVEFS*