AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016232

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016232 MtrunA17_Chr3g0126391 96.765 340 11 0 1 340 1 340 0.0 683
MsaG016232 MtrunA17_Chr8g0387981 37.330 367 180 15 7 340 12 361 3.71e-55 184
MsaG016232 MtrunA17_Chr7g0275531 34.746 354 173 12 7 340 12 327 1.64e-50 171
MsaG016232 MtrunA17_Chr8g0347251 35.833 360 170 15 7 334 12 342 9.57e-46 159
MsaG016232 MtrunA17_Chr7g0244981 33.333 204 102 4 2 197 9 186 7.07e-26 104
MsaG016232 MtrunA17_Chr7g0244971 32.487 197 97 5 7 194 15 184 8.07e-25 102
MsaG016232 MtrunA17_Chr8g0334541 32.273 220 120 6 8 227 6 196 4.24e-21 91.7
MsaG016232 MtrunA17_Chr3g0128671 32.579 221 109 10 13 221 23 215 1.15e-20 90.9
MsaG016232 MtrunA17_Chr8g0350621 29.000 200 116 4 1 198 1 176 4.55e-20 88.2
MsaG016232 MtrunA17_Chr2g0315371 31.220 205 106 7 5 192 14 200 7.06e-19 85.5
MsaG016232 MtrunA17_Chr1g0147881 40.441 136 54 8 84 200 207 334 2.33e-18 84.7
MsaG016232 MtrunA17_Chr5g0428351 42.697 89 51 0 104 192 154 242 3.80e-18 84.0
MsaG016232 MtrunA17_Chr5g0431811 42.683 82 45 1 125 206 664 743 1.22e-17 84.3
MsaG016232 MtrunA17_Chr2g0315361 31.034 203 116 5 5 192 14 207 3.99e-17 80.9
MsaG016232 MtrunA17_Chr2g0315381 39.773 88 45 1 105 192 124 203 4.82e-17 80.5
MsaG016232 MtrunA17_Chr8g0348311 42.105 95 50 2 134 225 156 248 1.08e-16 79.7
MsaG016232 MtrunA17_Chr5g0401821 44.828 87 44 3 104 190 172 254 1.56e-16 78.6
MsaG016232 MtrunA17_Chr7g0248691 31.746 189 120 4 103 284 124 310 3.78e-16 78.2
MsaG016232 MtrunA17_Chr8g0387501 51.562 64 29 1 127 190 123 184 4.11e-16 77.4
MsaG016232 MtrunA17_Chr4g0053281 41.509 106 48 6 101 199 236 334 4.94e-16 77.8
MsaG016232 MtrunA17_Chr1g0184691 56.140 57 23 1 134 190 294 348 3.45e-15 75.9
MsaG016232 MtrunA17_Chr7g0270051 54.839 62 26 1 129 190 49 108 5.82e-15 73.9
MsaG016232 MtrunA17_Chr8g0379461 55.000 60 25 1 131 190 74 131 1.18e-14 72.8
MsaG016232 MtrunA17_Chr7g0218081 47.692 65 30 2 126 190 221 281 1.56e-14 74.3
MsaG016232 MtrunA17_Chr7g0218081 50.847 59 26 1 132 190 409 464 1.99e-13 71.2
MsaG016232 MtrunA17_Chr2g0319961 39.823 113 54 3 86 190 29 135 1.74e-14 72.4
MsaG016232 MtrunA17_Chr4g0071521 36.522 115 65 3 81 190 189 300 1.86e-14 73.2
MsaG016232 MtrunA17_Chr2g0284321 51.515 66 27 2 128 190 27 90 2.41e-14 71.2
MsaG016232 MtrunA17_Chr8g0335811 52.542 59 25 1 132 190 153 208 2.49e-14 71.2
MsaG016232 MtrunA17_Chr2g0293481 43.689 103 50 4 134 231 268 367 2.68e-14 73.6
MsaG016232 MtrunA17_Chr8g0383351 52.542 59 25 1 132 190 159 214 2.82e-14 71.2
MsaG016232 MtrunA17_Chr7g0224801 53.333 60 25 1 132 191 92 148 3.26e-14 69.7
MsaG016232 MtrunA17_Chr7g0238921 55.738 61 24 1 130 190 121 178 3.31e-14 72.4
MsaG016232 MtrunA17_Chr7g0270831 51.351 74 33 2 118 190 200 271 4.73e-14 72.8
MsaG016232 MtrunA17_Chr3g0129361 52.542 59 25 1 132 190 143 198 9.11e-14 69.7
MsaG016232 MtrunA17_Chr1g0202461 41.176 85 42 2 106 190 240 316 1.40e-13 71.2
MsaG016232 MtrunA17_Chr7g0270191 51.667 60 26 1 132 191 144 200 1.69e-13 70.1
MsaG016232 MtrunA17_Chr7g0249311 53.448 58 23 2 133 190 179 232 1.81e-13 71.2
MsaG016232 MtrunA17_Chr4g0051881 44.928 69 35 1 132 200 177 242 1.96e-13 70.5
MsaG016232 MtrunA17_Chr5g0418781 52.542 59 25 1 132 190 123 178 2.54e-13 69.3
MsaG016232 MtrunA17_Chr5g0443131 49.206 63 27 2 132 192 156 215 3.74e-13 69.3
MsaG016232 MtrunA17_Chr1g0150801 50.000 60 27 1 132 191 101 157 4.79e-13 66.2
MsaG016232 MtrunA17_Chr2g0322391 54.237 59 25 1 132 190 348 404 5.50e-13 69.7
MsaG016232 MtrunA17_Chr4g0042831 50.000 58 26 1 133 190 145 199 5.72e-13 69.3
MsaG016232 MtrunA17_Chr4g0042831 47.458 59 28 1 132 190 277 332 3.53e-11 63.9
MsaG016232 MtrunA17_Chr3g0079691 51.667 60 25 2 131 190 278 333 5.95e-13 69.7
MsaG016232 MtrunA17_Chr3g0079691 33.628 113 63 3 132 240 494 598 4.93e-12 67.0
MsaG016232 MtrunA17_Chr7g0228941 50.877 57 26 1 134 190 53 107 5.99e-13 65.1
MsaG016232 MtrunA17_Chr1g0194481 50.000 60 27 1 132 191 157 213 1.23e-12 67.8
MsaG016232 MtrunA17_Chr5g0445311 49.153 59 27 1 132 190 363 418 1.37e-12 68.6
MsaG016232 MtrunA17_Chr5g0445311 47.059 68 30 3 125 190 190 253 2.62e-11 64.7
MsaG016232 MtrunA17_Chr5g0418191 46.479 71 33 3 121 190 138 204 1.51e-12 68.2
MsaG016232 MtrunA17_Chr5g0418191 49.153 59 27 1 132 190 319 374 2.29e-12 67.8
MsaG016232 MtrunA17_Chr2g0288871 52.542 59 26 1 132 190 304 360 1.61e-12 68.2
MsaG016232 MtrunA17_Chr2g0294231 50.000 60 27 1 132 191 202 258 1.62e-12 67.8
MsaG016232 MtrunA17_Chr7g0263531 34.454 119 58 5 87 190 302 415 1.78e-12 67.8
MsaG016232 MtrunA17_Chr7g0263531 50.000 60 26 2 131 190 187 242 8.83e-12 65.9
MsaG016232 MtrunA17_Chr3g0101131 49.153 59 27 1 132 190 414 469 2.27e-12 67.8
MsaG016232 MtrunA17_Chr3g0101131 47.692 65 30 2 126 190 236 296 2.12e-11 65.1
MsaG016232 MtrunA17_Chr4g0075201 51.667 60 27 1 132 191 266 323 2.53e-12 67.8
MsaG016232 MtrunA17_Chr3g0139021 50.877 57 26 1 134 190 315 369 2.99e-12 67.0
MsaG016232 MtrunA17_Chr8g0386241 44.068 59 30 1 132 190 102 157 3.64e-12 64.3
MsaG016232 MtrunA17_Chr7g0249301 52.542 59 24 2 132 190 61 115 4.29e-12 66.6
MsaG016232 MtrunA17_Chr1g0150281 52.632 57 25 1 134 190 141 195 4.35e-12 65.9
MsaG016232 MtrunA17_Chr2g0302391 34.247 146 70 5 134 260 212 350 6.71e-12 66.2
MsaG016232 MtrunA17_Chr5g0432161 50.000 58 25 2 133 190 244 297 1.04e-11 65.9
MsaG016232 MtrunA17_Chr5g0432161 49.153 59 27 1 132 190 420 475 1.99e-11 65.1
MsaG016232 MtrunA17_Chr3g0106491 46.032 63 31 1 132 194 364 423 1.41e-11 65.5
MsaG016232 MtrunA17_Chr3g0106491 50.000 58 25 2 133 190 198 251 1.68e-11 65.1
MsaG016232 MtrunA17_Chr7g0243891 50.000 58 25 2 133 190 216 269 1.70e-11 65.1
MsaG016232 MtrunA17_Chr4g0068541 47.458 59 28 1 132 190 207 262 1.86e-11 64.7
MsaG016232 MtrunA17_Chr4g0004911 40.909 88 48 2 104 190 306 390 2.10e-11 64.7
MsaG016232 MtrunA17_Chr4g0004911 46.552 58 28 2 133 190 184 238 6.56e-11 63.2
MsaG016232 MtrunA17_Chr5g0447701 47.541 61 29 1 130 190 249 306 2.28e-11 64.3
MsaG016232 MtrunA17_Chr1g0150271 32.381 105 66 2 132 236 100 199 6.90e-11 62.0
MsaG016232 MtrunA17_Chr7g0216891 43.243 74 39 2 118 190 203 274 7.69e-11 63.2
MsaG016232 MtrunA17_Chr3g0136961 47.458 59 29 1 132 190 137 193 8.11e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016232 AT4G11070.1 35.043 351 166 11 3 336 7 312 8.38e-47 161
MsaG016232 AT4G23810.1 35.688 269 137 8 3 248 10 265 4.33e-46 159
MsaG016232 AT5G24110.1 40.179 224 98 7 5 223 14 206 1.59e-42 149
MsaG016232 AT4G11070.2 32.647 340 157 10 3 336 7 280 2.24e-40 143
MsaG016232 AT2G46400.1 30.631 333 162 12 1 319 2 279 7.16e-32 121
MsaG016232 AT2G40750.1 32.301 226 116 9 5 207 13 224 3.39e-25 104
MsaG016232 AT3G56400.1 33.333 186 107 3 7 192 11 179 4.36e-24 100
MsaG016232 AT2G40740.1 59.677 62 25 0 131 192 171 232 5.39e-22 94.7
MsaG016232 AT2G40740.3 59.677 62 25 0 131 192 180 241 6.73e-22 94.4
MsaG016232 AT5G01900.1 28.846 208 123 5 5 211 6 189 3.31e-17 80.5
MsaG016232 AT5G45260.1 59.649 57 23 0 134 190 1209 1265 9.55e-17 82.0
MsaG016232 AT1G66550.1 28.125 192 97 4 8 192 10 167 2.13e-16 78.2
MsaG016232 AT1G64000.1 40.777 103 50 4 104 203 89 183 4.16e-16 76.3
MsaG016232 AT2G23320.1 46.667 90 38 5 104 190 213 295 5.38e-16 78.2
MsaG016232 AT5G52830.1 48.837 86 36 2 106 191 144 221 5.51e-16 78.2
MsaG016232 AT1G66600.1 29.557 203 104 5 8 205 10 178 7.14e-16 76.6
MsaG016232 AT1G66550.2 28.649 185 100 3 8 192 10 162 8.56e-16 76.3
MsaG016232 AT1G29280.1 56.667 60 24 1 131 190 72 129 1.07e-15 76.3
MsaG016232 AT5G41570.1 47.297 74 34 2 132 203 97 167 1.30e-15 74.3
MsaG016232 AT5G49520.1 53.333 60 25 1 132 191 220 276 1.66e-15 77.4
MsaG016232 AT4G24240.1 40.187 107 60 3 84 190 234 336 1.81e-15 77.0
MsaG016232 AT4G01250.1 33.577 137 70 4 126 261 121 237 2.12e-15 76.3
MsaG016232 AT2G46130.1 45.946 74 35 2 132 203 29 99 3.74e-15 71.2
MsaG016232 AT4G31550.2 56.140 57 23 1 134 190 246 300 4.61e-15 75.5
MsaG016232 AT4G31550.1 56.140 57 23 1 134 190 247 301 4.70e-15 75.5
MsaG016232 AT2G03340.1 33.548 155 85 7 45 190 158 303 5.81e-15 76.3
MsaG016232 AT2G03340.1 38.202 89 52 1 132 220 414 499 6.66e-12 66.6
MsaG016232 AT5G07100.2 41.414 99 49 3 111 203 113 208 6.71e-15 73.2
MsaG016232 AT5G07100.2 46.552 58 28 1 133 190 24 78 1.46e-11 63.5
MsaG016232 AT5G07100.4 41.414 99 49 3 111 203 113 208 6.71e-15 73.2
MsaG016232 AT5G07100.4 46.552 58 28 1 133 190 24 78 1.46e-11 63.5
MsaG016232 AT5G07100.5 41.414 99 49 3 111 203 113 208 6.71e-15 73.2
MsaG016232 AT5G07100.5 46.552 58 28 1 133 190 24 78 1.46e-11 63.5
MsaG016232 AT5G07100.3 41.414 99 49 3 111 203 113 208 6.71e-15 73.2
MsaG016232 AT5G07100.3 46.552 58 28 1 133 190 24 78 1.46e-11 63.5
MsaG016232 AT5G07100.1 41.414 99 49 3 111 203 206 301 7.83e-15 74.7
MsaG016232 AT5G07100.1 46.552 58 28 1 133 190 117 171 1.26e-11 65.1
MsaG016232 AT2G30590.1 43.617 94 42 5 100 190 283 368 1.85e-14 73.9
MsaG016232 AT4G26440.1 53.846 65 26 2 126 190 171 231 1.98e-14 74.7
MsaG016232 AT4G26440.1 47.619 63 30 1 132 194 371 430 1.63e-11 65.9
MsaG016232 AT4G23550.1 50.000 70 33 1 121 190 128 195 2.20e-14 73.2
MsaG016232 AT5G28650.1 40.179 112 54 3 79 190 219 317 2.39e-14 73.6
MsaG016232 AT2G24570.1 56.140 57 23 1 134 190 244 298 2.68e-14 73.2
MsaG016232 AT2G44745.1 52.542 59 25 1 132 190 144 199 2.81e-14 71.6
MsaG016232 AT4G04450.2 45.745 94 46 3 100 190 162 253 8.62e-14 72.4
MsaG016232 AT5G22570.1 46.154 65 35 0 134 198 111 175 9.55e-14 71.2
MsaG016232 AT4G18170.1 46.377 69 34 1 132 200 171 236 9.98e-14 71.2
MsaG016232 AT3G04670.1 54.386 57 24 1 134 190 263 317 1.18e-13 71.2
MsaG016232 AT3G58710.1 55.000 60 25 1 131 190 69 126 1.27e-13 70.5
MsaG016232 AT3G58710.2 55.000 60 25 1 131 190 68 125 1.30e-13 70.5
MsaG016232 AT2G34830.1 56.140 57 23 1 134 190 216 270 1.32e-13 71.6
MsaG016232 AT2G34830.2 56.140 57 23 1 134 190 225 279 1.42e-13 71.6
MsaG016232 AT5G26170.1 49.153 59 27 1 132 190 112 167 1.90e-13 68.2
MsaG016232 AT1G13960.1 42.169 83 43 3 108 190 205 282 2.08e-13 71.2
MsaG016232 AT1G13960.1 49.153 59 27 1 132 190 408 463 2.23e-12 68.2
MsaG016232 AT4G04450.1 45.745 94 46 3 100 190 256 347 2.09e-13 71.2
MsaG016232 AT2G37260.1 46.377 69 34 1 122 190 338 403 2.33e-13 70.9
MsaG016232 AT2G37260.3 46.377 69 34 1 122 190 258 323 2.33e-13 70.5
MsaG016232 AT2G37260.2 46.377 69 34 1 122 190 256 321 2.35e-13 70.5
MsaG016232 AT1G13960.2 42.169 83 43 3 108 190 52 129 3.23e-13 70.1
MsaG016232 AT1G13960.2 49.153 59 27 1 132 190 255 310 2.46e-12 67.8
MsaG016232 AT3G01080.3 53.333 60 24 2 131 190 203 258 3.52e-13 69.7
MsaG016232 AT1G29860.1 46.377 69 34 1 132 200 135 200 4.50e-13 68.9
MsaG016232 AT3G01080.2 53.333 60 24 2 131 190 203 258 6.73e-13 69.7
MsaG016232 AT3G01080.2 39.560 91 47 2 105 190 311 398 5.49e-12 67.0
MsaG016232 AT3G01080.1 53.333 60 24 2 131 190 165 220 7.58e-13 69.3
MsaG016232 AT3G01080.1 39.560 91 47 2 105 190 273 360 6.06e-12 66.6
MsaG016232 AT1G80590.1 28.646 192 87 5 8 199 10 151 7.71e-13 67.8
MsaG016232 AT4G01720.1 54.237 59 25 1 132 190 238 294 1.01e-12 69.3
MsaG016232 AT1G30650.1 56.140 57 23 1 134 190 218 272 1.02e-12 68.9
MsaG016232 AT1G69310.4 49.153 59 27 1 132 190 146 201 1.12e-12 68.2
MsaG016232 AT1G69310.2 49.153 59 27 1 132 190 146 201 1.12e-12 68.2
MsaG016232 AT1G69310.1 49.153 59 27 1 132 190 146 201 1.12e-12 68.2
MsaG016232 AT1G66560.1 45.000 60 33 0 133 192 103 162 1.14e-12 67.4
MsaG016232 AT5G46350.1 45.455 66 33 1 132 197 182 244 2.01e-12 67.8
MsaG016232 AT2G25000.2 50.000 62 29 1 132 193 102 161 2.58e-12 66.2
MsaG016232 AT2G25000.4 50.000 62 29 1 132 193 102 161 2.58e-12 66.2
MsaG016232 AT5G56270.1 49.231 65 29 2 126 190 266 326 2.81e-12 68.2
MsaG016232 AT1G62300.1 47.297 74 36 2 118 190 296 367 3.37e-12 67.8
MsaG016232 AT4G26640.7 49.153 59 27 1 132 190 340 395 3.52e-12 67.8
MsaG016232 AT4G26640.7 50.000 58 25 2 133 190 171 224 9.18e-11 63.2
MsaG016232 AT2G25000.3 50.000 62 29 1 132 193 124 183 3.53e-12 65.9
MsaG016232 AT2G25000.1 50.000 62 29 1 132 193 145 204 3.78e-12 66.2
MsaG016232 AT2G04880.2 49.153 59 27 1 132 190 282 337 3.84e-12 67.4
MsaG016232 AT4G26640.2 49.153 59 27 1 132 190 380 435 4.20e-12 67.4
MsaG016232 AT4G26640.1 49.153 59 27 1 132 190 308 363 4.45e-12 67.4
MsaG016232 AT2G04880.1 49.153 59 27 1 132 190 306 361 4.46e-12 67.4
MsaG016232 AT4G26640.5 49.153 59 27 1 132 190 204 259 5.37e-12 66.6
MsaG016232 AT4G26640.6 49.153 59 27 1 132 190 204 259 5.37e-12 66.6
MsaG016232 AT4G26640.4 49.153 59 27 1 132 190 204 259 5.37e-12 66.6
MsaG016232 AT4G26640.3 49.153 59 27 1 132 190 204 259 5.37e-12 66.6
MsaG016232 AT5G13080.1 50.000 60 27 1 132 191 66 122 6.65e-12 63.2
MsaG016232 AT4G39410.1 49.153 59 27 1 132 190 222 277 6.69e-12 65.9
MsaG016232 AT3G01970.1 50.000 60 27 1 132 191 64 120 7.29e-12 63.2
MsaG016232 AT4G22070.4 52.542 59 26 1 132 190 296 352 1.16e-11 66.2
MsaG016232 AT4G22070.3 52.542 59 26 1 132 190 296 352 1.16e-11 66.2
MsaG016232 AT4G22070.2 52.542 59 26 1 132 190 296 352 1.16e-11 66.2
MsaG016232 AT4G22070.1 52.542 59 26 1 132 190 296 352 1.16e-11 66.2
MsaG016232 AT2G47260.1 48.333 60 28 1 132 191 173 229 1.20e-11 65.5
MsaG016232 AT3G62340.1 50.000 60 27 1 132 191 117 173 1.77e-11 64.3
MsaG016232 AT5G43290.1 49.180 61 28 1 130 190 111 168 2.22e-11 63.9
MsaG016232 AT2G38470.1 47.458 59 28 1 132 190 361 416 2.83e-11 65.1
MsaG016232 AT1G68150.1 44.304 79 39 3 132 207 234 310 4.01e-11 63.9
MsaG016232 AT4G12020.3 37.079 89 51 3 102 190 438 521 6.06e-11 64.3
MsaG016232 AT4G12020.10 37.079 89 51 3 102 190 438 521 6.11e-11 64.3
MsaG016232 AT4G12020.6 37.079 89 51 3 102 190 438 521 6.23e-11 64.3
MsaG016232 AT4G12020.8 37.079 89 51 3 102 190 438 521 6.23e-11 64.3
MsaG016232 AT4G12020.1 37.079 89 51 3 102 190 438 521 6.36e-11 64.3
MsaG016232 AT4G12020.9 37.079 89 51 3 102 190 438 521 6.36e-11 64.3
MsaG016232 AT4G12020.7 37.079 89 51 3 102 190 438 521 6.76e-11 64.3
MsaG016232 AT4G12020.4 37.079 89 51 3 102 190 438 521 6.87e-11 64.3
MsaG016232 AT5G64810.1 40.299 67 37 1 132 198 109 172 6.88e-11 61.2
MsaG016232 AT4G12020.2 37.079 89 51 3 102 190 438 521 7.06e-11 64.3
MsaG016232 AT4G12020.5 37.079 89 51 3 102 190 371 454 7.75e-11 63.9
MsaG016232 AT2G40740.2 57.500 40 17 0 153 192 149 188 9.37e-11 62.0
MsaG016232 AT4G12020.11 37.079 89 51 3 102 190 438 521 9.91e-11 63.5

Find 75 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATCTCACTTGCCATATT+TGG 0.088426 3:+95643618 None:intergenic
CAAATTTACATACCTCTTTC+TGG 0.143241 3:+95644560 None:intergenic
CTGTAAAAGACATGTTAATA+TGG 0.191166 3:-95644715 MsaT016232.1:CDS
TTTGATCAATTTGAATTTGA+TGG 0.254177 3:-95643543 MsaT016232.1:CDS
TGTTAACAATATTTGTTTCT+TGG 0.254224 3:+95643877 None:intergenic
TATGTTTCCCCTGCAAATTC+AGG 0.272093 3:-95643681 MsaT016232.1:CDS
ATGGAACAAATGATTGGTTT+AGG 0.306745 3:+95643757 None:intergenic
AAACACACCAACTGCTTCTA+TGG 0.316690 3:-95643574 MsaT016232.1:CDS
AATCTCACGTGTTTCATTAG+AGG 0.318717 3:+95644788 None:intergenic
GTTAACAATATTTGTTTCTT+GGG 0.321184 3:+95643878 None:intergenic
ATGATTGGTTTAGGAAATCT+AGG 0.323422 3:+95643766 None:intergenic
GGACCTTCCACTGCCATTCC+TGG 0.341337 3:+95644233 None:intergenic
TGACTTATTCCTGAATTTGC+AGG 0.343271 3:+95643672 None:intergenic
GGAACACTCTAGCAATTAGA+TGG 0.350756 3:-95644277 MsaT016232.1:CDS
TAATCTCACTTGCCATATTT+GGG 0.350967 3:+95643619 None:intergenic
GGGTATAGTTGGCGAAAATA+TGG 0.351304 3:-95644200 MsaT016232.1:CDS
TTTGTTTCTTGGGTTTGAAA+TGG 0.354628 3:+95643888 None:intergenic
TTTCAAATGGAACAAATGAT+TGG 0.363750 3:+95643751 None:intergenic
ATTTGAAAAGGCACTTGAAA+TGG 0.370230 3:-95644742 MsaT016232.1:CDS
TCAAATTCAAATTGATCAAA+TGG 0.396441 3:+95643546 None:intergenic
TAGAAACGTTCTAGGATGTT+TGG 0.398748 3:-95644024 MsaT016232.1:CDS
TTCAAAAGTTGTTCATTTGG+TGG 0.409814 3:+95643825 None:intergenic
CAAAGCTACTTTCAAGTACA+TGG 0.413007 3:+95643727 None:intergenic
TTGTTCAAAAGTTGTTCATT+TGG 0.416222 3:+95643822 None:intergenic
TGCAGTCATAGAAACGTTCT+AGG 0.419203 3:-95644032 MsaT016232.1:CDS
AGTTGTGGAATGTGAAGTTT+TGG 0.442534 3:+95643520 None:intergenic
CTGAGGCTGAAGGGTATTGT+TGG 0.449383 3:+95644679 None:intergenic
TATGTGATTTCAAAGATAGT+TGG 0.452782 3:+95643969 None:intergenic
TGAAATGGATTAGTTAATGT+TGG 0.458156 3:+95643903 None:intergenic
AAGGTGGCGAATCTGATATT+CGG 0.465518 3:+95644643 None:intergenic
TTTGTTGATCAAGGCTTTGA+GGG 0.467796 3:+95643850 None:intergenic
TTTGGGAAAACACTTGGAGT+TGG 0.470835 3:+95643636 None:intergenic
AATTATATCCACATTTGAAA+AGG 0.478087 3:-95644754 MsaT016232.1:CDS
AGATAAGCTACTACAAGGAT+TGG 0.478321 3:-95644853 MsaT016232.1:CDS
TGTAAAAGACATGTTAATAT+GGG 0.479565 3:-95644714 MsaT016232.1:CDS
GTGGAAGGTCCTCTTGATGA+TGG 0.481430 3:-95644221 MsaT016232.1:CDS
TTCTGGAAGCATTGTGATCA+CGG 0.489343 3:+95644577 None:intergenic
ATGTGATTTCAAAGATAGTT+GGG 0.493028 3:+95643970 None:intergenic
CAACAATACCCTTCAGCCTC+AGG 0.495514 3:-95644678 MsaT016232.1:CDS
GCCATATTTGGGAAAACACT+TGG 0.500966 3:+95643630 None:intergenic
ATCACAATGCTTCCAGAAAG+AGG 0.506811 3:-95644572 MsaT016232.1:intron
GACTTATTCCTGAATTTGCA+GGG 0.514420 3:+95643673 None:intergenic
ACCTTGGATGCACGGCTCCA+AGG 0.515275 3:+95644165 None:intergenic
CTCTTGATGATGGGTATAGT+TGG 0.516255 3:-95644211 MsaT016232.1:CDS
TCCTTGGAGCCGTGCATCCA+AGG 0.519224 3:-95644166 MsaT016232.1:intron
CTGTCGGAGTCTTCACTTCG+CGG 0.524147 3:+95644609 None:intergenic
TTGTGCATGTGTGGTTTCCT+CGG 0.524687 3:+95643946 None:intergenic
TATGGTCAGAAAGACATCCT+TGG 0.530226 3:-95644182 MsaT016232.1:CDS
GCAAGTGTTCCTGAGGCTGA+AGG 0.534387 3:+95644669 None:intergenic
GTGTTTGCAGCAGAAGTAGC+TGG 0.543085 3:+95643591 None:intergenic
CAAGTGTTCCTGAGGCTGAA+GGG 0.573717 3:+95644670 None:intergenic
GCTACTACAAGGATTGGAGT+TGG 0.576309 3:-95644847 MsaT016232.1:CDS
AATTATATACCTTGGATGCA+CGG 0.579367 3:+95644157 None:intergenic
TGGAAGGTCCTCTTGATGAT+GGG 0.583275 3:-95644220 MsaT016232.1:CDS
TATTCGAGTTGAACCAGGAA+TGG 0.589228 3:-95644246 MsaT016232.1:CDS
AGTTGAACCAGGAATGGCAG+TGG 0.591297 3:-95644240 MsaT016232.1:CDS
TTTGCAGGGGAAACATATGA+AGG 0.596062 3:+95643687 None:intergenic
ATCACGGTTAAAATCTCTGT+CGG 0.596477 3:+95644593 None:intergenic
GAACCAGGAATGGCAGTGGA+AGG 0.596663 3:-95644236 MsaT016232.1:CDS
TTAATAGATAAGCTACTACA+AGG 0.612960 3:-95644858 MsaT016232.1:CDS
AAACATATTCGAGTTGAACC+AGG 0.613692 3:-95644251 MsaT016232.1:CDS
GAACTCTTAGACCTGTTCGT+AGG 0.617613 3:+95643799 None:intergenic
CTTCGCGGACTACAGCTCAA+AGG 0.617709 3:+95644624 None:intergenic
TAATGTTGGACCAACCACAT+TGG 0.618042 3:+95643917 None:intergenic
GGAAACTCCATAGAAGCAGT+TGG 0.622336 3:+95643567 None:intergenic
TGGAAGCAATTGTGCATGTG+TGG 0.623042 3:+95643937 None:intergenic
CAACTATACCCATCATCAAG+AGG 0.624580 3:+95644212 None:intergenic
ACAATTGCTTCCAATGTGGT+TGG 0.627124 3:-95643927 MsaT016232.1:CDS
TGGCGAATCTGATATTCGGA+TGG 0.634201 3:+95644647 None:intergenic
ATCTTTGAAATCACATACCG+AGG 0.643767 3:-95643963 MsaT016232.1:CDS
CGCGGACTACAGCTCAAAGG+TGG 0.645554 3:+95644627 None:intergenic
TCGGATGGCAAGTGTTCCTG+AGG 0.646934 3:+95644662 None:intergenic
ACTTATTCCTGAATTTGCAG+GGG 0.668793 3:+95643674 None:intergenic
ATGCACAATTGCTTCCAATG+TGG 0.717502 3:-95643931 MsaT016232.1:CDS
TTTGCAGCAGAAGTAGCTGG+TGG 0.728180 3:+95643594 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 95643503 95644898 95643503 ID=MsaG016232
Chr3 mRNA 95643503 95644898 95643503 ID=MsaT016232.1;Parent=MsaG016232
Chr3 exon 95643503 95644067 95643503 ID=MsaT016232.1.exon3;Parent=MsaT016232.1
Chr3 CDS 95643503 95644067 95643503 ID=cds.MsaT016232.1;Parent=MsaT016232.1
Chr3 exon 95644167 95644298 95644167 ID=MsaT016232.1.exon2;Parent=MsaT016232.1
Chr3 CDS 95644167 95644298 95644167 ID=cds.MsaT016232.1;Parent=MsaT016232.1
Chr3 exon 95644573 95644898 95644573 ID=MsaT016232.1.exon1;Parent=MsaT016232.1
Chr3 CDS 95644573 95644898 95644573 ID=cds.MsaT016232.1;Parent=MsaT016232.1
Gene Sequence

>MsaT016232.1

ATGGGAGATTCAAAGAACTTAATAGATAAGCTACTACAAGGATTGGAGTTGGCAAGACAGCTTCAAATCTATCTCAACGTGTCATCTTCCTCTAATGAAACACGTGAGATTTTGATCCAGAAAATTATATCCACATTTGAAAAGGCACTTGAAATGGTTAACTGTAAAAGACATGTTAATATGGGTGAATCTTCACACCAACAATACCCTTCAGCCTCAGGAACACTTGCCATCCGAATATCAGATTCGCCACCTTTGAGCTGTAGTCCGCGAAGTGAAGACTCCGACAGAGATTTTAACCGTGATCACAATGCTTCCAGAAAGAGGAACACTCTAGCAATTAGATGGACAAAACATATTCGAGTTGAACCAGGAATGGCAGTGGAAGGTCCTCTTGATGATGGGTATAGTTGGCGAAAATATGGTCAGAAAGACATCCTTGGAGCCGTGCATCCAAGAGGCTATTACAGATGCAGTCATAGAAACGTTCTAGGATGTTTGGCAACAAAGCAAGTACAAAGATCTGATGAAGACCCAACTATCTTTGAAATCACATACCGAGGAAACCACACATGCACAATTGCTTCCAATGTGGTTGGTCCAACATTAACTAATCCATTTCAAACCCAAGAAACAAATATTGTTAACACAAACCCTCAAAGCCTTGATCAACAAAAACCACCAAATGAACAACTTTTGAACAACCTACGAACAGGTCTAAGAGTTCAAACTGAGAACCTAGATTTCCTAAACCAATCATTTGTTCCATTTGAAAACCATGTACTTGAAAGTAGCTTTGCTGAAAATTTCAATTCTCCTTCATATGTTTCCCCTGCAAATTCAGGAATAAGTCACTTCTCAATGTCTCCAACTCCAAGTGTTTTCCCAAATATGGCAAGTGAGATTATTCCACCAGCTACTTCTGCTGCAAACACACCAACTGCTTCTATGGAGTTTCCATTTGATCAATTTGAATTTGATGGCCAAAACTTCACATTCCACAACTCACGATTTTTCTCTTAA
Protein sequence

>MsaT016232.1

MGDSKNLIDKLLQGLELARQLQIYLNVSSSSNETREILIQKIISTFEKALEMVNCKRHVNMGESSHQQYPSASGTLAIRISDSPPLSCSPRSEDSDRDFNRDHNASRKRNTLAIRWTKHIRVEPGMAVEGPLDDGYSWRKYGQKDILGAVHPRGYYRCSHRNVLGCLATKQVQRSDEDPTIFEITYRGNHTCTIASNVVGPTLTNPFQTQETNIVNTNPQSLDQQKPPNEQLLNNLRTGLRVQTENLDFLNQSFVPFENHVLESSFAENFNSPSYVSPANSGISHFSMSPTPSVFPNMASEIIPPATSAANTPTASMEFPFDQFEFDGQNFTFHNSRFFS*