AlfalfaGEDB | MsaG016290

M. sativa ssp. caerulea / MsaG016290

KEGG: -
GO: -
PFAM: PF02042.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016290	XP_024633826.2	86.667	270	23	4	1	266	1	261	3.86e-167	473

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016290	sp\|Q9LVU8\|RKD4_ARATH	57.143	105	45	0	159	263	148	252	1.23e-33	125

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016290	G7J7G0	86.667	270	23	4	1	266	1	261	1.84e-167	473

TFs/TRs:

Gene ID	Type	Classification
MsaG016290	TF	RWP-RK

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000722	MsaG016290	0.811085	1.431402e-50	2.510004e-48
MsaG002052	MsaG016290	0.803719	5.135824e-49	7.558378e-47
MsaG003214	MsaG016290	0.815775	1.347604e-51	2.656584e-49
MsaG003381	MsaG016290	0.813212	4.943552e-51	9.136713e-49
MsaG003726	MsaG016290	0.818310	3.654517e-52	7.688789e-50
MsaG003998	MsaG016290	0.809857	2.627865e-50	4.472190e-48
MsaG004353	MsaG016290	0.824207	1.618832e-53	3.984967e-51
MsaG004534	MsaG016290	0.825322	8.861865e-54	2.249090e-51
MsaG005105	MsaG016290	0.887586	2.727264e-72	7.101573e-69
MsaG005813	MsaG016290	0.814752	2.269330e-51	4.359466e-49
MsaG006334	MsaG016290	0.821820	5.795347e-53	1.337521e-50
MsaG006338	MsaG016290	0.814733	2.291515e-51	4.399854e-49
MsaG006431	MsaG016290	0.801892	1.219065e-48	1.720367e-46
MsaG006757	MsaG016290	0.842570	4.439013e-58	1.880106e-55
MsaG006944	MsaG016290	0.816940	7.415492e-52	1.506121e-49
MsaG006994	MsaG016290	0.861219	2.360114e-63	1.922120e-60
MsaG007737	MsaG016290	0.803899	4.713609e-49	6.965858e-47
MsaG007908	MsaG016290	0.834809	4.402331e-56	1.466240e-53
MsaG008995	MsaG016290	0.802772	8.047036e-49	1.158634e-46
MsaG009078	MsaG016290	0.843040	3.333496e-58	1.433230e-55
MsaG009345	MsaG016290	0.806410	1.413174e-49	2.214534e-47
MsaG009728	MsaG016290	0.805754	1.939620e-49	2.992637e-47
MsaG010065	MsaG016290	0.839826	2.318838e-57	9.003112e-55
MsaG010816	MsaG016290	0.828293	1.743621e-54	4.807382e-52
MsaG011177	MsaG016290	0.879880	1.831278e-69	3.286317e-66
MsaG011132	MsaG016290	0.816833	7.836306e-52	1.587155e-49
MsaG011386	MsaG016290	0.803224	6.495286e-49	9.450370e-47
MsaG012287	MsaG016290	0.808846	4.318528e-50	7.172645e-48
MsaG014214	MsaG016290	0.804199	4.085470e-49	6.079918e-47
MsaG015333	MsaG016290	0.810943	1.535575e-50	2.683325e-48
MsaG015721	MsaG016290	0.822259	4.592214e-53	1.072353e-50
MsaG015809	MsaG016290	0.800366	2.492408e-48	3.397871e-46
MsaG016058	MsaG016290	0.808339	5.535123e-50	9.081290e-48
MsaG016248	MsaG016290	0.811966	9.228210e-51	1.653593e-48
MsaG016290	MsaG016347	0.818201	3.866263e-52	8.111832e-50
MsaG016290	MsaG017318	0.806373	1.438694e-49	2.252533e-47
MsaG016290	MsaG017341	0.803206	6.551208e-49	9.527800e-47
MsaG016290	MsaG017722	0.841085	1.090739e-57	4.405866e-55
MsaG016290	MsaG018078	0.845594	6.918293e-59	3.232823e-56
MsaG016290	MsaG018249	0.839672	2.542799e-57	9.824561e-55
MsaG016290	MsaG019594	0.820825	9.807429e-53	2.204557e-50
MsaG016290	MsaG020336	0.827776	2.318725e-54	6.301080e-52
MsaG016290	MsaG020486	0.859543	7.545523e-63	5.765218e-60
MsaG016290	MsaG020497	0.808636	4.788229e-50	7.912237e-48
MsaG016290	MsaG020713	0.834849	4.301351e-56	1.434340e-53
MsaG016290	MsaG020815	0.851003	2.247940e-60	1.261158e-57
MsaG016290	MsaG020838	0.824043	1.768116e-53	4.333339e-51
MsaG016290	MsaG020889	0.820556	1.130348e-52	2.522841e-50
MsaG016290	MsaG021520	0.803908	4.693859e-49	6.938073e-47
MsaG016290	MsaG021523	0.813344	4.623841e-51	8.574018e-49
MsaG016290	MsaG021859	0.841983	6.341570e-58	2.635797e-55
MsaG016290	MsaG022894	0.803724	5.123355e-49	7.540944e-47
MsaG016290	MsaG023120	0.806785	1.179145e-49	1.864145e-47
MsaG016290	MsaG023405	0.818579	3.177147e-52	6.731508e-50
MsaG016290	MsaG023762	0.824894	1.117599e-53	2.803208e-51
MsaG016290	MsaG026487	0.805573	2.115577e-49	3.250276e-47
MsaG016290	MsaG026940	0.806738	1.206328e-49	1.905030e-47
MsaG016290	MsaG027138	0.809697	2.843025e-50	4.819675e-48
MsaG016290	MsaG027519	0.801476	1.482282e-48	2.072074e-46
MsaG016290	MsaG027591	0.829759	7.723208e-55	2.219767e-52
MsaG016290	MsaG028119	0.804301	3.891028e-49	5.804381e-47
MsaG016290	MsaG029963	0.806856	1.139010e-49	1.803670e-47
MsaG016290	MsaG032636	0.805381	2.320640e-49	3.549434e-47
MsaG016290	MsaG033534	0.807399	8.752137e-50	1.403881e-47
MsaG016290	MsaG033991	0.802179	1.064678e-48	1.512300e-46
MsaG016290	MsaG034824	0.829854	7.323535e-55	2.110698e-52
MsaG016290	MsaG035539	0.818959	2.607925e-52	5.580693e-50
MsaG016290	MsaG036045	0.834251	6.070650e-56	1.988338e-53
MsaG016290	MsaG036346	0.839863	2.268127e-57	8.816546e-55
MsaG016290	MsaG036417	0.817910	4.495986e-52	9.362335e-50
MsaG016290	MsaG037420	0.840464	1.583760e-57	6.273256e-55
MsaG016290	MsaG037871	0.812943	5.657170e-51	1.038573e-48
MsaG016290	MsaG038518	0.839126	3.517848e-57	1.336207e-54
MsaG016290	MsaG039679	0.818917	2.665827e-52	5.698186e-50
MsaG016290	MsaG040670	0.812656	6.535330e-51	1.191192e-48
MsaG016290	MsaG040713	0.865212	1.390964e-64	1.325452e-61
MsaG016290	MsaG041237	0.807335	9.028019e-50	1.445938e-47
MsaG016290	MsaG043772	0.817146	6.670382e-52	1.362010e-49
MsaG016290	MsaG043755	0.865926	8.300517e-65	8.137644e-62
MsaG016290	MsaG043926	0.834016	6.946018e-56	2.259122e-53
MsaG016290	MsaG044113	0.814202	2.999880e-51	5.683756e-49
MsaG016290	MsaG045016	0.814102	3.154976e-51	5.962637e-49
MsaG016290	MsaG045539	0.869377	6.576376e-66	7.424565e-63
MsaG016290	MsaG045689	0.814490	2.591781e-51	4.946118e-49
MsaG016290	MsaG046135	0.821875	5.630236e-53	1.301340e-50
MsaG016290	MsaG046538	0.860648	3.512719e-63	2.799800e-60
MsaG016290	MsaG046446	0.848758	9.471493e-60	4.920426e-57
MsaG016290	MsaG046733	0.804177	4.128010e-49	6.140092e-47
MsaG016290	MsaG001555	0.835420	3.092020e-56	1.048838e-53
MsaG016290	MsaG008209	0.853540	4.297783e-61	2.635523e-58
MsaG016290	MsaG013399	0.800166	2.736003e-48	3.713024e-46
MsaG016290	MsaG022889	0.803412	5.940623e-49	8.680951e-47
MsaG016290	MsaG023189	0.823367	2.541201e-53	6.114794e-51
MsaG016290	MsaG032663	0.815789	1.337964e-51	2.638593e-49
MsaG016290	MsaG034263	0.800739	2.093391e-48	2.878097e-46
MsaG016290	MsaG031242	0.817941	4.425193e-52	9.222389e-50
MsaG016290	MsaG030699	0.831839	2.401065e-55	7.328030e-53
MsaG016290	MsaG030436	0.810632	1.791853e-50	3.107470e-48
MsaG016290	MsaG031710	0.804219	4.045829e-49	6.023812e-47
MsaG016290	MsaG037087	0.895409	2.218289e-75	8.733247e-72
MsaG016290	MsaG036646	0.844613	1.270048e-58	5.745976e-56
MsaG016290	MsaG039900	0.824893	1.118371e-53	2.805057e-51
MsaG016290	MsaG039635	0.859718	6.687885e-63	5.144394e-60

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016290	MtrunA17_Chr3g0127001	86.667	270	23	4	1	266	1	261	3.55e-171	473
MsaG016290	MtrunA17_Chr1g0182631	85.833	240	25	3	30	266	1	234	2.78e-150	419
MsaG016290	MtrunA17_Chr4g0060761	52.778	108	42	2	164	262	160	267	5.55e-30	113
MsaG016290	MtrunA17_Chr3g0092141	47.321	112	53	1	159	264	59	170	9.98e-30	110
MsaG016290	MtrunA17_Chr5g0406901	39.200	125	58	2	153	259	190	314	9.35e-22	92.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016290	AT5G53040.1	57.143	105	45	0	159	263	148	252	1.25e-34	125
MsaG016290	AT5G66990.1	53.153	111	41	2	164	263	164	274	1.06e-31	118
MsaG016290	AT1G18790.1	50.435	115	47	2	156	260	125	239	4.77e-30	114
MsaG016290	AT1G74480.1	45.082	122	57	1	145	256	121	242	5.90e-29	111
MsaG016290	AT4G35590.2	33.333	135	70	2	143	257	169	303	1.43e-17	81.3
MsaG016290	AT4G35590.1	33.333	135	70	2	143	257	219	353	2.87e-17	80.9
MsaG016290	AT5G16100.2	36.082	97	54	1	147	235	342	438	2.54e-12	66.6

Find 45 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTAGCAGCCATTGTTAA+AGG	0.208533	3:+96395087	None:intergenic
TTGGAGCCTTGAGTGATTTC+AGG	0.214370	3:+96395964	None:intergenic
AATGTTGTCTTGCCTGAGTT+GGG	0.325768	3:-96395339	MsaT016290.1:CDS
CAATGTTGTCTTGCCTGAGT+TGG	0.335905	3:-96395340	MsaT016290.1:CDS
ATGATTTCTTTGAGTTTGAT+TGG	0.342407	3:-96395491	MsaT016290.1:CDS
GAAGATGTTGGAGAAACTTC+CGG	0.360968	3:-96394161	MsaT016290.1:CDS
GCCTTGAGTGATTTCAGGTT+GGG	0.378542	3:+96395969	None:intergenic
CAGCAATAGTCTCACCTTTG+TGG	0.390633	3:+96395307	None:intergenic
AAGATGTTGGAGAAACTTCC+GGG	0.393818	3:-96394160	MsaT016290.1:CDS
AGAATTGTCATTAGTTCCAT+TGG	0.417793	3:-96395205	MsaT016290.1:CDS
TCATCATTCAAATCCATTCC+CGG	0.427588	3:+96394142	None:intergenic
TTGTGAAGAAAGAGAAGGAA+TGG	0.429732	3:-96395236	MsaT016290.1:CDS
CAGAAAACTGAGGAGCTTGA+TGG	0.447810	3:-96394998	MsaT016290.1:CDS
CTCGTACTGCTACTTGTACT+TGG	0.454025	3:+96395177	None:intergenic
GGTACTCATCGACAACCTTA+AGG	0.457457	3:-96394977	MsaT016290.1:intron
GTTGGAGAAACTTCCGGGAA+TGG	0.462988	3:-96394155	MsaT016290.1:CDS
CCTCAAAATTATTCTGATCA+AGG	0.463476	3:+96395379	None:intergenic
TGATTTCTTTGAGTTTGATT+GGG	0.465378	3:-96395490	MsaT016290.1:CDS
TGTGAAGAAAGAGAAGGAAT+GGG	0.479018	3:-96395235	MsaT016290.1:CDS
AACTCAAAGAAATCATCAAA+TGG	0.481692	3:+96395498	None:intergenic
TGTTGTGTGATGCAGGAAAA+GGG	0.482015	3:-96394220	MsaT016290.1:intron
AAATCACTCAAGGCTCCAAC+TGG	0.487286	3:-96395960	MsaT016290.1:CDS
ATGATGAAATCAAGAAGCTT+AGG	0.499327	3:-96394126	MsaT016290.1:CDS
CTTGTACTTGGCAATGCCAA+TGG	0.515278	3:+96395189	None:intergenic
GGAGAAGCAAAAGAAGATGT+TGG	0.518512	3:-96394173	MsaT016290.1:CDS
GCTTCTGTGAAAACTGAAAG+TGG	0.520217	3:-96395261	MsaT016290.1:CDS
GACTAAGGAAGTTGCCATGT+TGG	0.527747	3:-96394194	MsaT016290.1:CDS
ATGTTGTCTTGCCTGAGTTG+GGG	0.533975	3:-96395338	MsaT016290.1:CDS
GCAGGAAAAGGGTTTGACTA+AGG	0.541768	3:-96394209	MsaT016290.1:intron
AGCCTTGAGTGATTTCAGGT+TGG	0.554392	3:+96395968	None:intergenic
TTGAACATTGTGATACCAGT+TGG	0.557847	3:+96395945	None:intergenic
TGGTTGTAATGCCCCAACTC+AGG	0.557980	3:+96395327	None:intergenic
AATTATTCTGATCAAGGTGT+GGG	0.571228	3:+96395385	None:intergenic
CTCAGGCAAGACAACATTGA+GGG	0.577274	3:+96395344	None:intergenic
GTACTAGCATCATTAAGAAG+AGG	0.607800	3:-96395155	MsaT016290.1:CDS
TATTCTGATCAAGGTGTGGG+AGG	0.607927	3:+96395388	None:intergenic
ACCCAACCTGAAATCACTCA+AGG	0.608828	3:-96395970	MsaT016290.1:CDS
AAATTATTCTGATCAAGGTG+TGG	0.609905	3:+96395384	None:intergenic
GCAGGGAGCTCAATATCACT+AGG	0.629741	3:-96395029	MsaT016290.1:CDS
GCTGCTAAGAAAATGAATGT+TGG	0.631864	3:-96395075	MsaT016290.1:CDS
TCAGGCAAGACAACATTGAG+GGG	0.654617	3:+96395345	None:intergenic
ACTCAGGCAAGACAACATTG+AGG	0.692460	3:+96395343	None:intergenic
GGGAGCTCAATATCACTAGG+TGG	0.701034	3:-96395026	MsaT016290.1:CDS
TTGGGGCATTACAACCACAA+AGG	0.718163	3:-96395321	MsaT016290.1:CDS
GGTGGCCTCACAGAAAACTG+AGG	0.738213	3:-96395008	MsaT016290.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	96394069	96396004	96394069	ID=MsaG016290
Chr3	mRNA	96394069	96396004	96394069	ID=MsaT016290.1;Parent=MsaG016290
Chr3	exon	96394069	96394227	96394069	ID=MsaT016290.1.exon3;Parent=MsaT016290.1
Chr3	CDS	96394069	96394227	96394069	ID=cds.MsaT016290.1;Parent=MsaT016290.1
Chr3	exon	96394978	96395536	96394978	ID=MsaT016290.1.exon2;Parent=MsaT016290.1
Chr3	CDS	96394978	96395536	96394978	ID=cds.MsaT016290.1;Parent=MsaT016290.1
Chr3	exon	96395922	96396004	96395922	ID=MsaT016290.1.exon1;Parent=MsaT016290.1
Chr3	CDS	96395922	96396004	96395922	ID=cds.MsaT016290.1;Parent=MsaT016290.1

Gene Sequence

>MsaT016290.1

ATGGACTTCTTCACCCAACCTGAAATCACTCAAGGCTCCAACTGGTATCACAATGTTCAACCTCAACAAAACGACACAGACAAAATCATGGATGTTGCGCCATTTGATGATTTCTTTGAGTTTGATTGGGCAACTCAATTTTCATACCAAAGTAATCATTTTTACATGAATGACTTCAATGATCTTGAAGATTTCAACTTTGATTTCAACCTCCCACACCTTGATCAGAATAATTTTGAGGTTGATCAAAAACCCCTCAATGTTGTCTTGCCTGAGTTGGGGCATTACAACCACAAAGGTGAGACTATTGCTGATGCTGAGAGTAGTTATGTTGAAGCTTCTGTGAAAACTGAAAGTGGTTTTGTGAAGAAAGAGAAGGAATGGGAGAATCTAGAATTGTCATTAGTTCCATTGGCATTGCCAAGTACAAGTAGCAGTACGAGTACTAGCATCATTAAGAAGAGGTCATCTTCATTGCAGTATGATGACATAAAGAAACATTTTGATGTTCCTTTAACAATGGCTGCTAAGAAAATGAATGTTGGTGTGACCCTTTTGAAAAAAAGATGCAGGGAGCTCAATATCACTAGGTGGCCTCACAGAAAACTGAGGAGCTTGATGGTACTCATCGACAACCTTAAGGAAAAGGGTTTGACTAAGGAAGTTGCCATGTTGGAGAAGCAAAAGAAGATGTTGGAGAAACTTCCGGGAATGGATTTGAATGATGAAATCAAGAAGCTTAGGCAAGCATGCTTCAAAGCTAATTACAAGAACAGAAAACTAATTGCTCTCTGTCCTTGA

Protein sequence

>MsaT016290.1

MDFFTQPEITQGSNWYHNVQPQQNDTDKIMDVAPFDDFFEFDWATQFSYQSNHFYMNDFNDLEDFNFDFNLPHLDQNNFEVDQKPLNVVLPELGHYNHKGETIADAESSYVEASVKTESGFVKKEKEWENLELSLVPLALPSTSSSTSTSIIKKRSSSLQYDDIKKHFDVPLTMAAKKMNVGVTLLKKRCRELNITRWPHRKLRSLMVLIDNLKEKGLTKEVAMLEKQKKMLEKLPGMDLNDEIKKLRQACFKANYKNRKLIALCP*