AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016416


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016416 MtrunA17_Chr3g0128471 91.829 257 16 3 1 254 1 255 7.45e-167 461
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016416 AT4G19985.6 48.052 231 89 5 17 223 14 237 3.25e-67 208
MsaG016416 AT4G19985.1 48.052 231 89 5 17 223 14 237 3.25e-67 208
MsaG016416 AT4G19985.4 42.205 263 89 6 17 223 14 269 5.05e-60 191
MsaG016416 AT4G19985.2 39.881 168 70 5 17 160 14 174 1.60e-30 112
MsaG016416 AT4G19985.5 39.881 168 70 5 17 160 14 174 1.60e-30 112
MsaG016416 AT4G19985.3 39.881 168 70 5 17 160 14 174 1.60e-30 112
MsaG016416 AT4G19985.7 39.181 171 73 5 17 163 14 177 1.90e-30 112

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAATCAATGGAATTAAAA+TGG 0.164701 3:+97681593 MsaT016416.1:CDS
GGTCGTGCTGTTTCTGATTT+TGG 0.179788 3:+97681973 MsaT016416.1:CDS
TGGATTTGGTGGTGACATTT+TGG 0.201084 3:+97683655 MsaT016416.1:CDS
TGACGGTTTGCTCACTTTCC+TGG 0.239344 3:-97683721 None:intergenic
TTTGGAGATGTTGAGGATTA+AGG 0.248880 3:-97681714 None:intergenic
CAGGAGTGTCGGAGATATTA+AGG 0.273878 3:-97681780 None:intergenic
TGCAAATGGAGCAGAGGTTT+TGG 0.278048 3:-97681732 None:intergenic
CCTCACCACATTGATGTTGA+TGG 0.296205 3:+97681874 MsaT016416.1:CDS
CCTGAACGTGATTTGTTGAT+TGG 0.298630 3:+97681946 MsaT016416.1:CDS
ATTAAGGTGGTGTGGGTTAG+TGG 0.360151 3:-97681698 None:intergenic
TTGTTGATATCAACTGGTTC+AGG 0.374194 3:-97681799 None:intergenic
CGTGATTTGTTGATTGGGTT+TGG 0.384811 3:+97681952 MsaT016416.1:CDS
CGAAGCTTGTTGATATCAAC+TGG 0.398400 3:-97681805 None:intergenic
CTGAACGTGATTTGTTGATT+GGG 0.419104 3:+97681947 MsaT016416.1:CDS
TGCTTCTATATACGATGTTA+TGG 0.428860 3:+97682002 MsaT016416.1:CDS
TATTCCATCCTTGAGGAGAA+TGG 0.433471 3:+97682621 MsaT016416.1:CDS
TCCTTGAGGAGAATGGGAAT+TGG 0.440922 3:+97682628 MsaT016416.1:CDS
ATTCCATCCTTGAGGAGAAT+GGG 0.454926 3:+97682622 MsaT016416.1:CDS
GTGTCGGAGATATTAAGGTT+GGG 0.458757 3:-97681775 None:intergenic
CCAATCAACAAATCACGTTC+AGG 0.463909 3:-97681946 None:intergenic
CCTCTCATCCTCTGAGCAAA+GGG 0.464970 3:-97683539 None:intergenic
GGAAATGAAAAGTGGGAGAA+GGG 0.466176 3:-97681677 None:intergenic
GAAATCAATGGAATTAAAAT+GGG 0.468872 3:+97681594 MsaT016416.1:CDS
AACAATAGCTTTCGGCCTGC+AGG 0.469985 3:-97683746 None:intergenic
AAATCACGTTCAGGAGAAAC+CGG 0.482910 3:-97681937 None:intergenic
TTTAAGGCTTGTGGATTTGG+TGG 0.483526 3:+97683644 MsaT016416.1:CDS
GAGCAAACCGTCACACCTGC+AGG 0.493036 3:+97683731 MsaT016416.1:CDS
TCCAATTCCCATTCTCCTCA+AGG 0.504293 3:-97682629 None:intergenic
GTGAAAAGAGTGGTTGCAAA+TGG 0.508457 3:-97681746 None:intergenic
GCGGAACCAACAATAGCTTT+CGG 0.512055 3:-97683754 None:intergenic
TGCAGGCCGAAAGCTATTGT+TGG 0.512613 3:+97683748 MsaT016416.1:CDS
AACTGTTTCATCAACAATCC+AGG 0.512965 3:+97683703 MsaT016416.1:CDS
TGTCGGAGATATTAAGGTTG+GGG 0.519931 3:-97681774 None:intergenic
CTTTCGGCCTGCAGGTGTGA+CGG 0.523798 3:-97683738 None:intergenic
ACCTCTCATCCTCTGAGCAA+AGG 0.534812 3:-97683540 None:intergenic
CCCTTTGCTCAGAGGATGAG+AGG 0.537381 3:+97683539 MsaT016416.1:CDS
AGTGTCGGAGATATTAAGGT+TGG 0.549755 3:-97681776 None:intergenic
ATCAACTGGTTCAGGAGTGT+CGG 0.554412 3:-97681791 None:intergenic
TGTTGAGGATTAAGGTGGTG+TGG 0.555356 3:-97681706 None:intergenic
AACAGTTCTCGTGTACATCA+TGG 0.561270 3:-97683687 None:intergenic
GTTGAGGATTAAGGTGGTGT+GGG 0.570563 3:-97681705 None:intergenic
TGGAAATGAAAAGTGGGAGA+AGG 0.570598 3:-97681678 None:intergenic
ATTCCCATTCTCCTCAAGGA+TGG 0.579989 3:-97682625 None:intergenic
GGAGATGTTGAGGATTAAGG+TGG 0.587846 3:-97681711 None:intergenic
TGCAGGTTATTCCATCCTTG+AGG 0.587883 3:+97682614 MsaT016416.1:intron
ACTCAATATTATCATCCTTG+AGG 0.593636 3:-97683800 None:intergenic
GGGTTAGTGGAAATGAAAAG+TGG 0.602812 3:-97681685 None:intergenic
GACAGATTCAAGAATCCTCA+AGG 0.611144 3:+97683785 MsaT016416.1:CDS
GAAATGAAAAGTGGGAGAAG+GGG 0.622002 3:-97681676 None:intergenic
CATAGCAGCCCTTTGCTCAG+AGG 0.625766 3:+97683531 MsaT016416.1:CDS
AGTGGTTGCAAATGGAGCAG+AGG 0.626606 3:-97681738 None:intergenic
TTTCGAAGCGAAAACTTCAG+TGG 0.633639 3:-97681575 None:intergenic
TTCTTGAATCTGTCAATCAG+AGG 0.641603 3:-97683776 None:intergenic
CCATCAACATCAATGTGGTG+AGG 0.651681 3:-97681874 None:intergenic
GGTTAGTGGAAATGAAAAGT+GGG 0.652172 3:-97681684 None:intergenic
TGGGGAATCTGTGAAAAGAG+TGG 0.656361 3:-97681756 None:intergenic
TTGAATCTGTCAATCAGAGG+CGG 0.671155 3:-97683773 None:intergenic
TGATTGTTAGAAAAGTTGTG+AGG 0.694957 3:+97682656 MsaT016416.1:CDS
TTAAACCATCAACATCAATG+TGG 0.739906 3:-97681879 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 97681550 97683811 97681550 ID=MsaG016416
Chr3 mRNA 97681550 97683811 97681550 ID=MsaT016416.1;Parent=MsaG016416
Chr3 exon 97681550 97682023 97681550 ID=MsaT016416.1.exon1;Parent=MsaT016416.1
Chr3 CDS 97681550 97682023 97681550 ID=cds.MsaT016416.1;Parent=MsaT016416.1
Chr3 exon 97682619 97682677 97682619 ID=MsaT016416.1.exon2;Parent=MsaT016416.1
Chr3 CDS 97682619 97682677 97682619 ID=cds.MsaT016416.1;Parent=MsaT016416.1
Chr3 exon 97683504 97683560 97683504 ID=MsaT016416.1.exon3;Parent=MsaT016416.1
Chr3 CDS 97683504 97683560 97683504 ID=cds.MsaT016416.1;Parent=MsaT016416.1
Chr3 exon 97683637 97683811 97683637 ID=MsaT016416.1.exon4;Parent=MsaT016416.1
Chr3 CDS 97683637 97683811 97683637 ID=cds.MsaT016416.1;Parent=MsaT016416.1
Gene Sequence

>MsaT016416.1

ATGGCGGCAACAGGAGTTGCAGCAGCCACTGAAGTTTTCGCTTCGAAATCAATGGAATTAAAATGGGTAAGAACAAGAACAAGAAGAATCACCAAAACCACACACAAACAAAAAACGCTATCAACACCCCTTCTCCCACTTTTCATTTCCACTAACCCACACCACCTTAATCCTCAACATCTCCAAAACCTCTGCTCCATTTGCAACCACTCTTTTCACAGATTCCCCAACCTTAATATCTCCGACACTCCTGAACCAGTTGATATCAACAAGCTTCGCATTGCTCTTTCTCATAGTGACGTTCTTGTCTCTGTCTTCTGTAAACCTCACCACATTGATGTTGATGGTTTAACTGAATCTTCATCTTCCATTGTTGATTTTTTCACGCCGGTTTCTCCTGAACGTGATTTGTTGATTGGGTTTGGTCGTGCTGTTTCTGATTTTGGTCTCACTGCTTCTATATACGATGTTATGGTTATTCCATCCTTGAGGAGAATGGGAATTGGAAAAATGATTGTTAGAAAAGTTGTGAGAATGCTTACAAATAGAGACATCTATGACATAGCAGCCCTTTGCTCAGAGGATGAGAGGTTGTTTTTTAAGGCTTGTGGATTTGGTGGTGACATTTTGGACTCTACTACCATGATGTACACGAGAACTGTTTCATCAACAATCCAGGAAAGTGAGCAAACCGTCACACCTGCAGGCCGAAAGCTATTGTTGGTTCCGCCTCTGATTGACAGATTCAAGAATCCTCAAGGATGA

Protein sequence

>MsaT016416.1

MAATGVAAATEVFASKSMELKWVRTRTRRITKTTHKQKTLSTPLLPLFISTNPHHLNPQHLQNLCSICNHSFHRFPNLNISDTPEPVDINKLRIALSHSDVLVSVFCKPHHIDVDGLTESSSSIVDFFTPVSPERDLLIGFGRAVSDFGLTASIYDVMVIPSLRRMGIGKMIVRKVVRMLTNRDIYDIAALCSEDERLFFKACGFGGDILDSTTMMYTRTVSSTIQESEQTVTPAGRKLLLVPPLIDRFKNPQG*