AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016470 MtrunA17_Chr3g0129071 100.000 115 0 0 1 115 30 144 2.49e-83 243
MsaG016470 MtrunA17_Chr5g0438091 88.496 113 13 0 6 118 27 139 8.04e-71 210
MsaG016470 MtrunA17_Chr5g0432451 82.051 117 19 1 1 115 24 140 1.98e-60 185
MsaG016470 MtrunA17_Chr2g0311601 70.103 97 29 0 15 111 10 106 1.31e-47 150
MsaG016470 MtrunA17_Chr5g0437571 72.632 95 26 0 17 111 5 99 6.49e-47 148
MsaG016470 MtrunA17_Chr4g0043421 69.474 95 29 0 17 111 14 108 3.99e-46 147
MsaG016470 MtrunA17_Chr3g0114061 69.388 98 30 0 14 111 4 101 5.34e-46 146
MsaG016470 MtrunA17_Chr7g0227511 66.667 96 32 0 17 112 10 105 5.57e-43 139
MsaG016470 MtrunA17_Chr4g0058651 66.667 96 32 0 17 112 10 105 1.08e-42 138
MsaG016470 MtrunA17_Chr6g0449641 64.583 96 34 0 17 112 8 103 1.68e-42 136
MsaG016470 MtrunA17_Chr3g0117121 66.667 96 32 0 17 112 9 104 2.21e-42 138
MsaG016470 MtrunA17_Chr3g0106101 62.745 102 38 0 17 118 6 107 2.24e-42 137
MsaG016470 MtrunA17_Chr3g0115431 61.000 100 39 0 17 116 8 107 5.14e-41 135
MsaG016470 MtrunA17_Chr6g0449651 62.500 96 36 0 17 112 66 161 9.56e-41 134
MsaG016470 MtrunA17_Chr5g0402611 63.542 96 35 0 17 112 7 102 3.09e-40 136
MsaG016470 MtrunA17_Chr3g0113891 60.000 100 40 0 17 116 13 112 2.44e-39 130
MsaG016470 MtrunA17_Chr7g0245871 63.107 103 37 1 18 119 27 129 3.36e-39 131
MsaG016470 MtrunA17_Chr3g0097221 58.000 100 42 0 17 116 6 105 8.82e-39 131
MsaG016470 MtrunA17_Chr4g0030001 60.825 97 37 1 16 111 22 118 7.59e-38 126
MsaG016470 MtrunA17_Chr1g0200201 61.290 93 36 0 19 111 73 165 2.42e-37 126
MsaG016470 MtrunA17_Chr5g0437401 58.000 100 42 0 17 116 10 109 3.58e-37 124
MsaG016470 MtrunA17_Chr8g0353981 64.634 82 29 0 17 98 6 87 3.85e-36 123
MsaG016470 MtrunA17_Chr7g0225111 53.000 100 47 0 19 118 6 105 4.26e-35 117
MsaG016470 MtrunA17_Chr7g0260971 57.143 98 41 1 15 111 11 108 5.43e-34 117
MsaG016470 MtrunA17_Chr6g0460951 54.455 101 45 1 16 115 38 138 1.72e-33 116
MsaG016470 MtrunA17_Chr1g0184281 58.947 95 38 1 18 111 15 109 2.34e-33 115
MsaG016470 MtrunA17_Chr8g0351721 55.882 102 44 1 18 118 18 119 4.73e-33 115
MsaG016470 MtrunA17_Chr5g0444351 52.000 100 48 0 19 118 6 105 1.20e-32 111
MsaG016470 MtrunA17_Chr7g0261031 55.882 102 42 2 17 116 7 107 1.25e-32 114
MsaG016470 MtrunA17_Chr8g0351701 57.576 99 41 1 18 115 23 121 3.00e-32 113
MsaG016470 MtrunA17_Chr1g0184271 55.446 101 42 2 18 116 12 111 3.11e-32 113
MsaG016470 MtrunA17_Chr5g0435641 49.515 103 51 1 17 118 6 108 7.93e-32 111
MsaG016470 MtrunA17_Chr8g0374601 49.000 100 51 0 16 115 24 123 3.16e-29 106
MsaG016470 MtrunA17_Chr8g0374591 43.000 100 57 0 16 115 6 105 1.27e-25 93.6
MsaG016470 MtrunA17_Chr8g0374581 47.525 101 52 1 16 115 6 106 5.19e-25 94.7
MsaG016470 MtrunA17_Chr7g0245861 53.333 90 39 2 18 104 12 101 6.51e-25 92.0
MsaG016470 MtrunA17_Chr6g0452691 38.614 101 61 1 19 118 10 110 6.68e-25 93.2
MsaG016470 MtrunA17_Chr6g0460941 46.067 89 47 1 18 105 7 95 1.18e-24 90.1
MsaG016470 MtrunA17_Chr6g0452671 37.736 106 66 0 14 119 7 112 1.40e-24 92.8
MsaG016470 MtrunA17_Chr6g0452661 36.364 110 66 2 13 118 1 110 9.30e-23 89.4
MsaG016470 MtrunA17_Chr4g0059901 37.736 106 66 0 15 120 7 112 2.72e-22 88.2
MsaG016470 MtrunA17_Chr4g0040831 36.735 98 62 0 19 116 16 113 7.37e-22 85.9
MsaG016470 MtrunA17_Chr2g0330201 40.187 107 62 2 15 120 16 121 2.28e-21 86.3
MsaG016470 MtrunA17_Chr8g0342351 37.864 103 62 2 15 116 9 110 1.21e-20 84.0
MsaG016470 MtrunA17_Chr5g0439111 34.545 110 68 2 13 118 1 110 5.97e-20 81.6
MsaG016470 MtrunA17_Chr4g0055441 35.644 101 64 1 19 118 3 103 1.61e-19 78.6
MsaG016470 MtrunA17_Chr2g0326041 60.784 51 20 0 16 66 31 81 1.51e-18 74.3
MsaG016470 MtrunA17_Chr4g0046201 34.091 88 58 0 17 104 5 92 2.34e-15 69.7
MsaG016470 MtrunA17_Chr2g0326051 61.224 49 19 0 63 111 5 53 3.50e-14 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016470 AT2G30340.1 79.167 120 23 2 1 118 33 152 3.55e-66 201
MsaG016470 AT2G30340.2 79.167 120 23 2 1 118 34 153 3.78e-66 201
MsaG016470 AT2G40470.2 89.655 116 12 0 3 118 5 120 2.53e-64 194
MsaG016470 AT2G40470.1 89.655 116 12 0 3 118 30 145 4.81e-64 194
MsaG016470 AT2G30130.1 66.667 105 31 1 7 111 1 101 2.61e-45 145
MsaG016470 AT3G27650.1 62.617 107 40 0 5 111 26 132 1.28e-43 140
MsaG016470 AT1G31320.1 72.727 88 24 0 17 104 12 99 2.50e-43 140
MsaG016470 AT1G16530.1 56.481 108 45 2 15 120 11 118 2.05e-41 135
MsaG016470 AT5G63090.4 64.583 96 34 0 17 112 10 105 4.08e-41 135
MsaG016470 AT5G63090.3 64.583 96 34 0 17 112 10 105 4.08e-41 135
MsaG016470 AT5G63090.2 64.583 96 34 0 17 112 10 105 4.08e-41 135
MsaG016470 AT5G63090.1 64.583 96 34 0 17 112 10 105 4.08e-41 135
MsaG016470 AT1G07900.1 61.458 96 37 0 16 111 31 126 3.54e-40 132
MsaG016470 AT5G66870.1 59.434 106 42 1 17 121 6 111 5.98e-40 135
MsaG016470 AT2G28500.1 61.458 96 37 0 16 111 53 148 6.41e-40 133
MsaG016470 AT2G23660.2 59.000 100 41 0 17 116 4 103 1.94e-38 131
MsaG016470 AT2G23660.3 59.000 100 41 0 17 116 4 103 1.94e-38 131
MsaG016470 AT2G23660.1 59.000 100 41 0 17 116 4 103 1.94e-38 131
MsaG016470 AT1G65620.1 67.059 85 28 0 14 98 5 89 6.20e-38 127
MsaG016470 AT1G65620.4 67.059 85 28 0 14 98 5 89 6.20e-38 127
MsaG016470 AT1G65620.3 67.059 85 28 0 14 98 5 89 6.20e-38 127
MsaG016470 AT1G65620.5 67.059 85 28 0 14 98 5 89 6.20e-38 127
MsaG016470 AT1G65620.2 67.059 85 28 0 14 98 5 89 6.20e-38 127
MsaG016470 AT3G26620.1 58.065 93 39 0 19 111 6 98 1.06e-36 121
MsaG016470 AT3G26660.1 58.065 93 39 0 19 111 6 98 1.18e-36 121
MsaG016470 AT3G11090.1 58.586 99 41 0 17 115 10 108 3.95e-36 121
MsaG016470 AT4G00210.1 60.000 95 37 1 18 111 11 105 1.38e-33 116
MsaG016470 AT2G42430.1 50.893 112 54 1 8 118 5 116 4.78e-33 116
MsaG016470 AT3G58190.1 55.446 101 42 2 18 116 11 110 8.52e-33 114
MsaG016470 AT2G31310.1 52.475 101 47 1 17 116 6 106 1.58e-32 113
MsaG016470 AT3G03760.1 56.000 100 43 1 17 115 50 149 2.70e-32 114
MsaG016470 AT5G06080.1 54.545 99 44 1 17 114 6 104 2.14e-31 110
MsaG016470 AT4G00220.1 58.333 96 39 1 21 115 20 115 3.46e-31 110
MsaG016470 AT2G45420.1 54.167 96 43 1 21 115 40 135 5.57e-30 108
MsaG016470 AT2G42440.1 50.000 102 48 2 17 116 6 106 1.31e-29 107
MsaG016470 AT2G45410.1 50.980 102 49 1 21 121 19 120 1.12e-28 103
MsaG016470 AT3G50510.1 43.434 99 56 0 17 115 11 109 1.22e-26 98.6
MsaG016470 AT3G50510.2 43.434 99 56 0 17 115 11 109 1.22e-26 98.6
MsaG016470 AT1G72980.1 41.509 106 62 0 15 120 10 115 4.67e-24 92.0
MsaG016470 AT3G47870.1 40.206 97 58 0 15 111 33 129 6.42e-23 91.3
MsaG016470 AT5G35900.1 38.542 96 59 0 17 112 4 99 1.05e-20 83.2
MsaG016470 AT3G13850.1 36.458 96 61 0 19 114 37 132 4.93e-20 82.8
MsaG016470 AT1G06280.1 48.052 77 40 0 19 95 25 101 5.37e-20 81.3
MsaG016470 AT2G19820.1 33.333 96 63 1 18 112 12 107 2.01e-16 70.1
MsaG016470 AT1G36000.1 30.526 95 64 2 18 111 9 102 7.11e-11 55.8

Find 24 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAGGGGTTATAGTATTT+AGG 0.181231 3:+98270944 None:intergenic
GGGTTCATGAGGAGAGAAAT+AGG 0.290029 3:+98270870 None:intergenic
AGGCTAAGAGATCCAGTTTA+TGG 0.306813 3:-98270641 MsaT016470.1:CDS
ACAAGGGGTTATAGTATTTA+GGG 0.313905 3:+98270945 None:intergenic
TGTGAACAGAAGCAAACTTC+TGG 0.320592 3:+98270848 None:intergenic
GTTATAGTATTTAGGGTTCC+TGG 0.351578 3:+98270952 None:intergenic
GCACCCATGCATCCATAAAC+TGG 0.381798 3:+98270629 None:intergenic
GGAGAGAAATAGGGAGAAAA+GGG 0.384566 3:+98270880 None:intergenic
AGGAGAGAAATAGGGAGAAA+AGG 0.419838 3:+98270879 None:intergenic
GTGAACAGAAGCAAACTTCT+GGG 0.421575 3:+98270849 None:intergenic
GCAGAACTCAATCTATTAAG+AGG 0.440766 3:-98270569 MsaT016470.1:intron
AATTTCTGCATTGCAACAAC+AGG 0.457176 3:-98270606 MsaT016470.1:CDS
ATGGGAAGAAGACATATGTT+AGG 0.472989 3:-98270979 MsaT016470.1:CDS
GGTTCATGAGGAGAGAAATA+GGG 0.495022 3:+98270871 None:intergenic
AGATCCAGTTTATGGATGCA+TGG 0.526865 3:-98270633 MsaT016470.1:CDS
AATAGCTTACATGCTGCACA+AGG 0.566069 3:+98270928 None:intergenic
GATCCAGTTTATGGATGCAT+GGG 0.578062 3:-98270632 MsaT016470.1:CDS
GCAAACTTCTGGGGTTCATG+AGG 0.589878 3:+98270859 None:intergenic
TAGCTTACATGCTGCACAAG+GGG 0.599040 3:+98270930 None:intergenic
ATAGTATTTAGGGTTCCTGG+TGG 0.604329 3:+98270955 None:intergenic
TGAACAGAAGCAAACTTCTG+GGG 0.623533 3:+98270850 None:intergenic
AGACATATGTTAGGTCCACC+AGG 0.627473 3:-98270970 MsaT016470.1:CDS
TTGTTTATGAAGCAAATGTG+AGG 0.672469 3:-98270661 MsaT016470.1:CDS
ATAGCTTACATGCTGCACAA+GGG 0.680325 3:+98270929 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 98269381 98271001 98269381 ID=MsaG016470
Chr3 mRNA 98269381 98271001 98269381 ID=MsaT016470.1;Parent=MsaG016470
Chr3 exon 98269381 98269403 98269381 ID=MsaT016470.1.exon3;Parent=MsaT016470.1
Chr3 CDS 98269381 98269403 98269381 ID=cds.MsaT016470.1;Parent=MsaT016470.1
Chr3 exon 98270570 98270723 98270570 ID=MsaT016470.1.exon2;Parent=MsaT016470.1
Chr3 CDS 98270570 98270723 98270570 ID=cds.MsaT016470.1;Parent=MsaT016470.1
Chr3 exon 98270810 98271001 98270810 ID=MsaT016470.1.exon1;Parent=MsaT016470.1
Chr3 CDS 98270810 98271001 98270810 ID=cds.MsaT016470.1;Parent=MsaT016470.1
Gene Sequence

>MsaT016470.1

ATGGGAAGAAGACATATGTTAGGTCCACCAGGAACCCTAAATACTATAACCCCTTGTGCAGCATGTAAGCTATTAAGAAGAAGATGTGCTCAAGAGTGTCCCTTTTCTCCCTATTTCTCTCCTCATGAACCCCAGAAGTTTGCTTCTGTTCACAAAGTTTTTGGTGCCAGCAACGTCTCAAAAATGCTAATGGAGGTAGCAGAATGTTCAAGAGCAGATGCAGCAAATAGTCTTGTTTATGAAGCAAATGTGAGGCTAAGAGATCCAGTTTATGGATGCATGGGTGCAATTTCTGCATTGCAACAACAGGTTCAATCTTTACAAGCAGAACTCAATCTATTAAGAGAAATTATAGATGTCATCTTTTGA
Protein sequence

>MsaT016470.1

MGRRHMLGPPGTLNTITPCAACKLLRRRCAQECPFSPYFSPHEPQKFASVHKVFGASNVSKMLMEVAECSRADAANSLVYEANVRLRDPVYGCMGAISALQQQVQSLQAELNLLREIIDVIF*