Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016533 | AES72826.1 | 95.890 | 73 | 3 | 0 | 1 | 73 | 45 | 117 | 5.83e-44 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016533 | sp|B9RU15|ATXR5_RICCO | 69.697 | 66 | 20 | 0 | 1 | 66 | 44 | 109 | 1.30e-29 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016533 | G7JB04 | 95.890 | 73 | 3 | 0 | 1 | 73 | 45 | 117 | 2.78e-44 | 147 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG016533 | TR | PHD |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016533 | MtrunA17_Chr3g0129871 | 95.890 | 73 | 3 | 0 | 1 | 73 | 5 | 77 | 1.31e-47 | 145 |
| MsaG016533 | MtrunA17_Chr3g0129881 | 77.049 | 61 | 14 | 0 | 3 | 63 | 60 | 120 | 1.72e-31 | 112 |
| MsaG016533 | MtrunA17_Chr1g0146731 | 54.167 | 48 | 22 | 0 | 16 | 63 | 26 | 73 | 3.36e-16 | 70.9 |
| MsaG016533 | MtrunA17_Chr5g0397501 | 49.091 | 55 | 28 | 0 | 11 | 65 | 237 | 291 | 4.57e-14 | 65.1 |
| MsaG016533 | MtrunA17_Chr1g0146761 | 50.000 | 48 | 20 | 1 | 16 | 63 | 73 | 116 | 1.14e-12 | 60.8 |
| MsaG016533 | MtrunA17_Chr6g0451001 | 41.818 | 55 | 32 | 0 | 9 | 63 | 1238 | 1292 | 9.76e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016533 | AT5G09790.2 | 66.038 | 53 | 18 | 0 | 14 | 66 | 62 | 114 | 7.39e-22 | 87.0 |
| MsaG016533 | AT5G09790.1 | 66.038 | 53 | 18 | 0 | 14 | 66 | 62 | 114 | 7.64e-22 | 86.7 |
| MsaG016533 | AT5G24330.1 | 66.667 | 48 | 16 | 0 | 17 | 64 | 33 | 80 | 8.54e-19 | 78.2 |
| MsaG016533 | AT1G63490.2 | 43.750 | 64 | 36 | 0 | 2 | 65 | 225 | 288 | 8.57e-13 | 61.6 |
| MsaG016533 | AT1G63490.4 | 43.750 | 64 | 36 | 0 | 2 | 65 | 225 | 288 | 8.74e-13 | 61.6 |
Find 20 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGAGTTCCTATTGGTTCA+TGG | 0.294500 | 3:+99031518 | MsaT016533.1:CDS |
| GAAGTGTCTGAGACCGGTTC+TGG | 0.362102 | 3:+99031495 | MsaT016533.1:CDS |
| CCCCAAGTGTTGTTCTGAGC+TGG | 0.393884 | 3:+99031546 | MsaT016533.1:CDS |
| GGGCAAATCCATGAACCAAT+AGG | 0.417784 | 3:-99031526 | None:intergenic |
| CTCCAGCTCAGAACAACACT+TGG | 0.460665 | 3:-99031548 | None:intergenic |
| GTTCTGGCTAGAGTTCCTAT+TGG | 0.469825 | 3:+99031511 | MsaT016533.1:CDS |
| ATGTGTGAGCGATGTGGATC+TGG | 0.479905 | 3:+99031418 | MsaT016533.1:CDS |
| ATAGGAACTCTAGCCAGAAC+CGG | 0.497595 | 3:-99031508 | None:intergenic |
| TCACACAACAAAAGCTCCTC+AGG | 0.503103 | 3:-99031448 | None:intergenic |
| TCATATGAAGTGTCTGAGAC+CGG | 0.525057 | 3:+99031489 | MsaT016533.1:CDS |
| TCCAGCTCAGAACAACACTT+GGG | 0.544872 | 3:-99031547 | None:intergenic |
| ATATGCAGTGGTTGAGAAGG+AGG | 0.563550 | 3:+99031381 | MsaT016533.1:CDS |
| TAAATATGCAGTGGTTGAGA+AGG | 0.574113 | 3:+99031378 | MsaT016533.1:CDS |
| TTGTGTGACAAATGCGACAA+AGG | 0.628248 | 3:+99031463 | MsaT016533.1:CDS |
| CGATGACTAATATAATGGCG+AGG | 0.630890 | 3:+99031353 | None:intergenic |
| GATGACTAATATAATGGCGA+GGG | 0.645664 | 3:+99031354 | None:intergenic |
| GGCGAGGGCTAAATATGCAG+TGG | 0.659115 | 3:+99031369 | MsaT016533.1:CDS |
| CCAGCTCAGAACAACACTTG+GGG | 0.668343 | 3:-99031546 | None:intergenic |
| GACTGCATGTGTGAGCGATG+TGG | 0.685024 | 3:+99031412 | MsaT016533.1:CDS |
| TGGATCTGGTGAACAACCTG+AGG | 0.697540 | 3:+99031432 | MsaT016533.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 99031367 | 99031588 | 99031367 | ID=MsaG016533 |
| Chr3 | mRNA | 99031367 | 99031588 | 99031367 | ID=MsaT016533.1;Parent=MsaG016533 |
| Chr3 | exon | 99031367 | 99031588 | 99031367 | ID=MsaT016533.1.exon1;Parent=MsaT016533.1 |
| Chr3 | CDS | 99031367 | 99031588 | 99031367 | ID=cds.MsaT016533.1;Parent=MsaT016533.1 |
| Gene Sequence |
| Protein sequence |
