AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016539

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016539 MtrunA17_Chr3g0129941 92.901 986 29 3 1 969 1 962 0.0 1853
MsaG016539 MtrunA17_Chr8g0384131 46.286 700 295 17 292 969 131 771 6.43e-171 518
MsaG016539 MtrunA17_Chr8g0384131 53.150 254 91 6 1 244 1 236 1.62e-57 212
MsaG016539 MtrunA17_Chr2g0308451 45.833 96 52 0 874 969 1345 1440 1.20e-19 95.5
MsaG016539 MtrunA17_Chr2g0282581 45.238 84 46 0 886 969 800 883 1.01e-15 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016539 AT3G18640.3 34.192 427 182 15 579 969 305 668 7.57e-42 164
MsaG016539 AT3G18640.1 34.192 427 182 15 579 969 305 668 7.57e-42 164
MsaG016539 AT3G18640.2 34.192 427 182 15 579 969 305 668 7.57e-42 164
MsaG016539 AT2G10553.1 73.973 73 19 0 897 969 2 74 6.45e-30 114
MsaG016539 AT3G26850.2 48.235 85 41 1 888 969 173 257 3.10e-18 86.3
MsaG016539 AT3G26850.1 48.235 85 41 1 888 969 173 257 3.10e-18 86.3
MsaG016539 AT2G33835.1 44.872 78 42 1 893 969 502 579 3.96e-14 77.0

Find 239 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAACTATTCTCACGATTT+TGG 0.170219 3:-99119072 None:intergenic
GGAGCCCTCCTCGTGGTTTC+AGG 0.197192 3:+99118927 MsaT016539.1:CDS
AAACTTGGGTAATTCAATTA+AGG 0.210347 3:+99121234 MsaT016539.1:CDS
TTCATTGGATCATACTGTTT+TGG 0.221297 3:-99121097 None:intergenic
TCATCCCTTAATCTTCTAAT+AGG 0.222977 3:-99119937 None:intergenic
TAGTTCAGAATGGGCTTATT+TGG 0.226634 3:+99116505 MsaT016539.1:CDS
CGTGGTTTCAGGTGGGATTC+AGG 0.255538 3:+99118938 MsaT016539.1:CDS
AAGCAGAATGATACAGTTTC+TGG 0.256951 3:+99120108 MsaT016539.1:CDS
CATCATCAGGTCCAGCATTT+TGG 0.268653 3:-99121417 None:intergenic
CTCATGGGTAGCAGAGTATC+TGG 0.271167 3:-99119122 None:intergenic
TTTCATTTCCTTTGTTTGCT+TGG 0.279484 3:-99121440 None:intergenic
TGTCTCCTGATTGGAATTAT+AGG 0.280617 3:+99120375 MsaT016539.1:CDS
TTCTTGTGTCTGCGGAATAT+TGG 0.281117 3:-99121608 None:intergenic
GGAGCCCTATTAGAAGATTA+AGG 0.281152 3:+99119932 MsaT016539.1:CDS
CTCATGGGTAGTAGGATATC+TGG 0.284857 3:-99119584 None:intergenic
TCATTAGATCTACCACTCTT+TGG 0.290780 3:-99119532 None:intergenic
GAACCAAGTATTATGGATTC+AGG 0.293291 3:+99120830 MsaT016539.1:CDS
CTCATAGGTAGCAGAATATC+TGG 0.294314 3:-99119494 None:intergenic
ATGCCCCATCCATCCGTTAT+AGG 0.294462 3:-99120204 None:intergenic
TCATCAAAGGAATCTTATTA+TGG 0.303424 3:+99116563 MsaT016539.1:CDS
TCTGTTTATGTTCTTCCATA+TGG 0.306253 3:-99120412 None:intergenic
ACTCCATTAGTCCAAATAAA+AGG 0.309217 3:+99120019 MsaT016539.1:CDS
TTCACTAGAGAAAGGGAAAA+TGG 0.309831 3:+99119466 MsaT016539.1:CDS
TTTGATGAAAATCCCGTAAC+AGG 0.314545 3:-99116548 None:intergenic
CTCGTGGTTAGTAGGATATC+TGG 0.333203 3:-99119674 None:intergenic
TTTGCTCAGTAGATACTTCC+TGG 0.335470 3:-99120796 None:intergenic
TTTAAATTCTCATGAATTGA+AGG 0.339340 3:+99120991 MsaT016539.1:CDS
CACCGGAGAACCAAGTATTA+TGG 0.341165 3:+99120823 MsaT016539.1:CDS
TCAAAACACGGTGGTCAATA+AGG 0.346403 3:+99121309 MsaT016539.1:CDS
TTTGTTTGCTTGGCATCATC+AGG 0.350045 3:-99121430 None:intergenic
TTGATGCATGTGATTGAAAT+AGG 0.355075 3:-99121052 None:intergenic
GTCGAAATGGTAAACATTGT+AGG 0.360118 3:+99119884 MsaT016539.1:CDS
TGCTCAATACTATCATTCAT+TGG 0.363648 3:-99121112 None:intergenic
CAGGAGTTGATGGTAGAAAA+AGG 0.366286 3:+99118957 MsaT016539.1:CDS
GGTAGATCTAATGACGAATA+TGG 0.374619 3:+99119721 MsaT016539.1:CDS
CACGAGGAGGGCTCCGACTC+CGG 0.375893 3:-99118919 None:intergenic
ACCAAAATCGTGAGAATAGT+TGG 0.381560 3:+99119071 MsaT016539.1:CDS
TGGATATGGTAAAGTTTCTA+TGG 0.385533 3:+99119258 MsaT016539.1:CDS
TGATAAATTAAGACCTGTTA+CGG 0.389039 3:+99116535 MsaT016539.1:CDS
ATAAGAAGGAAGCTGATCTT+TGG 0.389807 3:+99120273 MsaT016539.1:intron
GAAGATTAAGGGATGATAGA+TGG 0.392675 3:+99119944 MsaT016539.1:CDS
GCCTCTCTGGCTCATATCCT+TGG 0.394980 3:+99120935 MsaT016539.1:CDS
TGTACAAGTTTGTTAAGCTT+TGG 0.396843 3:-99121661 None:intergenic
GGGAGTTCAACGGCAGGATC+TGG 0.399881 3:-99121076 None:intergenic
GTGATTGAAATAGGCATTGC+AGG 0.400850 3:-99121043 None:intergenic
GCCTTCTCTTGAAGAAAGAA+TGG 0.400963 3:-99120753 None:intergenic
ATAGGCAAATCAGTAAATTC+TGG 0.403354 3:-99120186 None:intergenic
GCTAGTGGAAATTGTCGAAA+TGG 0.404283 3:+99119871 MsaT016539.1:CDS
GGACTGTCCTCGACTCTATC+AGG 0.404783 3:-99120084 None:intergenic
ATTTACTGATTTGCCTATAA+CGG 0.405639 3:+99120191 MsaT016539.1:CDS
TGCATTGGCTGAGTTTGTTA+AGG 0.407254 3:+99121483 MsaT016539.1:CDS
CATCCCTTAATCTTCTAATA+GGG 0.409509 3:-99119936 None:intergenic
GATTACGATTCTGATGGATA+TGG 0.409902 3:+99119244 MsaT016539.1:CDS
GAAACTGTATCATTCTGCTT+TGG 0.421384 3:-99120105 None:intergenic
CGGTCCTCTGCAGATGTTGC+TGG 0.427388 3:+99116476 MsaT016539.1:CDS
TTTATGTTCTTCCATATGGC+TGG 0.428708 3:-99120408 None:intergenic
TGCAAAAGGCAGCTGTAGAA+TGG 0.429741 3:+99119198 MsaT016539.1:CDS
AACTTGGGTAATTCAATTAA+GGG 0.430799 3:+99121235 MsaT016539.1:CDS
CACGAATCTCTCAGCCTCTC+TGG 0.435442 3:+99120922 MsaT016539.1:CDS
ATCTACCACTCTTTGGAGAA+TGG 0.438405 3:-99119525 None:intergenic
CAATAGGTACAACGGACATT+TGG 0.441250 3:+99120336 MsaT016539.1:CDS
ATGCATGGTTCCCAGTCAAT+AGG 0.446023 3:+99120320 MsaT016539.1:CDS
AACATTTGATGATCTCTACT+TGG 0.448605 3:-99119976 None:intergenic
GATAAATTAAGACCTGTTAC+GGG 0.449771 3:+99116536 MsaT016539.1:CDS
TTCACTAGAGAAAGAGAAGT+TGG 0.452745 3:+99119763 MsaT016539.1:CDS
AGGTGGGATTCAGGAGTTGA+TGG 0.452955 3:+99118947 MsaT016539.1:CDS
AAAGTTGTTGATAAAGTAAC+GGG 0.454132 3:+99121571 MsaT016539.1:CDS
TTCATTAGAGAAAGAGAAGA+TGG 0.454567 3:+99119376 MsaT016539.1:CDS
CCCATTCTTTCTTCAAGAGA+AGG 0.459974 3:+99120752 MsaT016539.1:CDS
TTGTCCAACTGCATGGCAGC+TGG 0.462211 3:-99120714 None:intergenic
AGAATGGTCCCTGCTCTCAT+AGG 0.464550 3:-99119509 None:intergenic
GAAGGTAGGCACAGGGAAGA+TGG 0.467005 3:+99119094 MsaT016539.1:CDS
GATGGGGTCTTCCAGCCATA+TGG 0.468810 3:+99120397 MsaT016539.1:CDS
GGTAGCAAGCAAATGCGGTC+TGG 0.469238 3:+99116452 MsaT016539.1:CDS
CTAATGAATTCACTAGAGAA+AGG 0.471067 3:+99119458 MsaT016539.1:CDS
GCAAAAGGCAGCTGTAGAAT+GGG 0.474363 3:+99119199 MsaT016539.1:CDS
ATAAAAGGCTAAGAGATGAT+AGG 0.475061 3:+99120034 MsaT016539.1:CDS
CGGAATATTGGCCTCCTGGA+TGG 0.475692 3:-99121596 None:intergenic
ATCCTGCTACCCATGAGAGC+AGG 0.476316 3:+99119320 MsaT016539.1:CDS
ATCCTGCTACCCATGAGAGC+AGG 0.476316 3:+99119410 MsaT016539.1:CDS
ATCCATAATACTTGGTTCTC+CGG 0.477069 3:-99120825 None:intergenic
ATCCTACTAACCACGAGAGC+AGG 0.477279 3:+99119680 MsaT016539.1:CDS
TCCCTGCTCTCATGGGTAGC+AGG 0.477919 3:-99119322 None:intergenic
TCCCTGCTCTCATGGGTAGC+AGG 0.477919 3:-99119412 None:intergenic
CCCATCCTATAATTCCAATC+AGG 0.478103 3:-99120380 None:intergenic
AGGAGCTTCTGAAACCAACT+TGG 0.478431 3:+99121503 MsaT016539.1:CDS
TTCACTAGTGAAAGGGAAGA+TGG 0.479255 3:+99119556 MsaT016539.1:CDS
TTCACTAGTGAAAGGGAAGA+TGG 0.479255 3:+99119646 MsaT016539.1:CDS
TAACCCAGGAAGAGAAAACT+TGG 0.485595 3:+99121219 MsaT016539.1:CDS
CCTTCTCTTGAAGAAAGAAT+GGG 0.486829 3:-99120752 None:intergenic
GAGCCCTATTAGAAGATTAA+GGG 0.488698 3:+99119933 MsaT016539.1:CDS
GACCGCATTTGCTTGCTACC+AGG 0.490417 3:-99116449 None:intergenic
CAAAACATGGACCAAAATGC+TGG 0.493199 3:+99121406 MsaT016539.1:CDS
AGGTACAACGGACATTTGGC+CGG 0.493224 3:+99120340 MsaT016539.1:CDS
GAGAATAAAAGTGGAAATGT+AGG 0.496432 3:+99120153 MsaT016539.1:CDS
CTGAAACCAACTTGGAAAGA+AGG 0.496600 3:+99121511 MsaT016539.1:CDS
GAGAATGGTCCCTGCTCTCA+TGG 0.498339 3:-99119330 None:intergenic
GAGAATGGTCCCTGCTCTCA+TGG 0.498339 3:-99119420 None:intergenic
TATTCTGTTGTGTTCAGAGA+TGG 0.499211 3:-99120471 None:intergenic
TGTCCGTTGTACCTATTGAC+TGG 0.499259 3:-99120331 None:intergenic
GCCCTCCTCGTGGTTTCAGG+TGG 0.499663 3:+99118930 MsaT016539.1:CDS
ATCCTACTACCCATGAGAGC+AGG 0.503657 3:+99119590 MsaT016539.1:CDS
TCTGCGGAATATTGGCCTCC+TGG 0.504700 3:-99121600 None:intergenic
CATAAAAGTACCAAAGAGAA+TGG 0.505662 3:+99121379 MsaT016539.1:CDS
CTTTGGATAGCAGAAAATAC+TGG 0.505714 3:+99120290 MsaT016539.1:CDS
ATGCAGAAATGTCTCCTGAT+TGG 0.505794 3:+99120366 MsaT016539.1:CDS
CCAAAATCGTGAGAATAGTT+GGG 0.505813 3:+99119072 MsaT016539.1:CDS
TGGTAGCAATAGTTCAGAAT+GGG 0.505951 3:+99116496 MsaT016539.1:CDS
GAGAATAGTCCCTGCTCTCA+TGG 0.506671 3:-99119600 None:intergenic
TCCCTGCTCTCATGGGTAGT+AGG 0.506862 3:-99119592 None:intergenic
GAGAATGGTCAACACTCTCA+TGG 0.506959 3:-99119138 None:intergenic
TCAGAATGGGCTTATTTGGA+AGG 0.508080 3:+99116509 MsaT016539.1:CDS
CCCTCCTCGTGGTTTCAGGT+GGG 0.508118 3:+99118931 MsaT016539.1:CDS
TCCCTGCTCTCGTGGTTAGT+AGG 0.508353 3:-99119682 None:intergenic
GAAGGAACATGATGTAAGCC+AGG 0.509135 3:+99120433 MsaT016539.1:CDS
ATCTACCACTCTTAAGAGAA+TGG 0.511468 3:-99119153 None:intergenic
ATCTACCACTCTTAAGAGAA+TGG 0.511468 3:-99119345 None:intergenic
ATCTACCACTCTTAAGAGAA+TGG 0.511468 3:-99119435 None:intergenic
GCATCTAGCATACAGGGAAA+AGG 0.516914 3:+99122102 MsaT016539.1:intron
AAGCCTGAATCCATAATACT+TGG 0.517448 3:-99120833 None:intergenic
ATTTGCCTATAACGGATGGA+TGG 0.518017 3:+99120199 MsaT016539.1:CDS
TGGATGAGTTCACTAGAGAA+AGG 0.519337 3:+99119278 MsaT016539.1:CDS
AAAGCTTAACAAACTTGTAC+AGG 0.519903 3:+99121663 MsaT016539.1:CDS
AATCTAATCCATTTGCAGCC+TGG 0.521014 3:+99121286 MsaT016539.1:CDS
TCCTGATTGGAATTATAGGA+TGG 0.523046 3:+99120379 MsaT016539.1:CDS
GAATCTTATTATGGAGGCAG+AGG 0.523186 3:+99116572 MsaT016539.1:CDS
CTAATGAATTCACTAGTGAA+AGG 0.525451 3:+99119548 MsaT016539.1:CDS
CTAATGAATTCACTAGTGAA+AGG 0.525451 3:+99119638 MsaT016539.1:CDS
TAAAGTAACGGGTACCATCC+AGG 0.528168 3:+99121582 MsaT016539.1:CDS
AAGCTGATCGAGAATAAAAG+TGG 0.529647 3:+99120144 MsaT016539.1:CDS
ATTGTTGACATGAGTGGAGC+TGG 0.529969 3:+99116377 None:intergenic
ATTCTGCTACCTATGAGAGC+AGG 0.529969 3:+99119500 MsaT016539.1:CDS
TCATGGGTAGTAGGATATCT+GGG 0.532080 3:-99119583 None:intergenic
ACGTGCATTTAAATTTGCAT+TGG 0.534604 3:+99121468 MsaT016539.1:CDS
TAATGAATTCACTAGAGAAA+GGG 0.537037 3:+99119459 MsaT016539.1:CDS
TAATGAATTCACTAGTGAAA+GGG 0.537448 3:+99119549 MsaT016539.1:CDS
TAATGAATTCACTAGTGAAA+GGG 0.537448 3:+99119639 MsaT016539.1:CDS
TCGTGGTTAGTAGGATATCT+GGG 0.538819 3:-99119673 None:intergenic
TTGCCAGGAACAGTCGGAGC+CGG 0.539425 3:+99118900 MsaT016539.1:intron
CTGGCTCATATCCTTGGAGC+AGG 0.539793 3:+99120941 MsaT016539.1:CDS
AGAAGAGAATGGGACACTGA+TGG 0.540407 3:+99116644 MsaT016539.1:CDS
ACTGATTTGCCTATAACGGA+TGG 0.544476 3:+99120195 MsaT016539.1:CDS
GAAAGTTGTTGATAAAGTAA+CGG 0.545410 3:+99121570 MsaT016539.1:CDS
CTGGTAGCAATAGTTCAGAA+TGG 0.546984 3:+99116495 MsaT016539.1:CDS
GGATGAGTTCACTAGAGAAA+GGG 0.551833 3:+99119279 MsaT016539.1:CDS
GTGCATGATTACGATTCTGA+TGG 0.551916 3:+99119238 MsaT016539.1:CDS
TTTGCCTATAACGGATGGAT+GGG 0.554623 3:+99120200 MsaT016539.1:CDS
ATAGTTGGGAAGGTAGGCAC+AGG 0.554939 3:+99119086 MsaT016539.1:CDS
TAACAATGAAGAGGTAGCTA+AGG 0.555412 3:+99121333 MsaT016539.1:CDS
AAGGCTAAGAGATGATAGGT+GGG 0.557111 3:+99120038 MsaT016539.1:CDS
CTTGACTTTGTCCAACTGCA+TGG 0.557475 3:-99120721 None:intergenic
CCTGATTGGAATTATAGGAT+GGG 0.560325 3:+99120380 MsaT016539.1:CDS
GGTACAACGGACATTTGGCC+GGG 0.563103 3:+99120341 MsaT016539.1:CDS
ACTTCCAGCTGCCATGCAGT+TGG 0.563914 3:+99120710 MsaT016539.1:CDS
TCCTACTACCCATGAGAGCA+GGG 0.565414 3:+99119591 MsaT016539.1:CDS
TGTTGATGCTATACACAGCC+AGG 0.565431 3:+99120778 MsaT016539.1:CDS
AGAATAGTCCCTGCTCTCAT+GGG 0.565639 3:-99119599 None:intergenic
AGAATGGTCAACACTCTCAT+GGG 0.572749 3:-99119137 None:intergenic
GGAACAGTCGGAGCCGGAGT+CGG 0.575027 3:+99118906 MsaT016539.1:intron
GTGAGAATAGTTGGGAAGGT+AGG 0.576320 3:+99119080 MsaT016539.1:CDS
AGCTCTATTGTTGACATGAG+TGG 0.576612 3:+99116371 None:intergenic
GCTGTTGGAAAATGTAGAAG+AGG 0.576715 3:+99119019 MsaT016539.1:CDS
CTGATTGGAATTATAGGATG+GGG 0.577469 3:+99120381 MsaT016539.1:CDS
AGAATGGTCCCTGCTCTCAT+GGG 0.581139 3:-99119329 None:intergenic
AGAATGGTCCCTGCTCTCAT+GGG 0.581139 3:-99119419 None:intergenic
TCCTGCTACCCATGAGAGCA+GGG 0.581326 3:+99119321 MsaT016539.1:CDS
TCCTGCTACCCATGAGAGCA+GGG 0.581326 3:+99119411 MsaT016539.1:CDS
TCATGGGTAGCAGAGTATCT+GGG 0.581368 3:-99119121 None:intergenic
GGAGACATTTCTGCATCACC+CGG 0.581677 3:-99120359 None:intergenic
GCAGCTGCTGACCTGCTCCA+AGG 0.582262 3:-99120952 None:intergenic
TCAAAGGAATCTTATTATGG+AGG 0.583027 3:+99116566 MsaT016539.1:CDS
CAGAAAGCACTCTTCAAAGT+GGG 0.584050 3:+99116400 MsaT016539.1:CDS
GTCCGTTGTACCTATTGACT+GGG 0.584696 3:-99120330 None:intergenic
TGATCATTGCCAGGAACAGT+CGG 0.587729 3:+99118894 MsaT016539.1:intron
AAAGGCTAAGAGATGATAGG+TGG 0.588164 3:+99120037 MsaT016539.1:CDS
ATGAAGAGGTAGCTAAGGAA+AGG 0.588800 3:+99121338 MsaT016539.1:CDS
TCCTACTAACCACGAGAGCA+GGG 0.589630 3:+99119681 MsaT016539.1:CDS
TTTACTTGGAGGAATGGCTG+GGG 0.589692 3:-99121161 None:intergenic
AACAGAATACAACTTGCTCC+AGG 0.591084 3:+99120485 MsaT016539.1:CDS
AAGCAAACAAAGGAAATGAA+AGG 0.591168 3:+99121442 MsaT016539.1:CDS
AATACTGGTGCCAACATGCA+TGG 0.593621 3:+99120305 MsaT016539.1:CDS
TTCTGCTACCTATGAGAGCA+GGG 0.594783 3:+99119501 MsaT016539.1:CDS
ATCACTCTTGACATGTTCTG+AGG 0.596680 3:-99121263 None:intergenic
TCATAGGTAGCAGAATATCT+GGG 0.598574 3:-99119493 None:intergenic
CTGCGACAGAATTTCTACTT+CGG 0.600476 3:-99121191 None:intergenic
TAGTGCGGTTAGGCACATGC+CGG 0.601071 3:-99119825 None:intergenic
AGAGAATGGTCCTCAAAACA+TGG 0.604039 3:+99121393 MsaT016539.1:CDS
GGAGCATCAATAGTGCGGTT+AGG 0.607218 3:-99119835 None:intergenic
AATCGTGAGAATAGTTGGGA+AGG 0.607787 3:+99119076 MsaT016539.1:CDS
TTACTTGGAGGAATGGCTGG+GGG 0.607949 3:-99121160 None:intergenic
GCAGAAAGCACTCTTCAAAG+TGG 0.608415 3:+99116399 MsaT016539.1:CDS
TTGCCTATAACGGATGGATG+GGG 0.608657 3:+99120201 MsaT016539.1:CDS
GCAAGCAAATGCGGTCTGGA+CGG 0.609119 3:+99116456 MsaT016539.1:CDS
TATGGAAGAACATAAACAGA+AGG 0.610346 3:+99120415 MsaT016539.1:CDS
TGCAGTTGGACAAAGTCAAG+TGG 0.611494 3:+99120724 MsaT016539.1:CDS
CTCACGATACGCAAGCATGT+AGG 0.613211 3:+99119911 MsaT016539.1:CDS
GTTGACCATTCTCTTAAGAG+TGG 0.613457 3:+99119148 MsaT016539.1:CDS
ATCAGTAAATTCTGGCTCTG+TGG 0.613647 3:-99120178 None:intergenic
ACACTGATGGAAGTTACAGC+AGG 0.614680 3:+99116657 MsaT016539.1:CDS
GAGAAAGAGAAGTTGGCAGA+AGG 0.615126 3:+99119770 MsaT016539.1:CDS
AAGTAGAGATCATCAAATGT+TGG 0.616100 3:+99119978 MsaT016539.1:CDS
CAATAAGGATAACAATGAAG+AGG 0.623098 3:+99121324 MsaT016539.1:CDS
AAGAATAGTCCCTGCTCTCG+TGG 0.625384 3:-99119690 None:intergenic
CTGTCGCAGTTGTCTAACCC+AGG 0.633065 3:+99121205 MsaT016539.1:CDS
TGATGATGCCAAGCAAACAA+AGG 0.634153 3:+99121432 MsaT016539.1:CDS
TGCAAGGAGCATCAATAGTG+CGG 0.638490 3:-99119840 None:intergenic
GATGAGCATAAATTCTCACC+TGG 0.639966 3:+99116431 MsaT016539.1:CDS
AGGGACTATTCTCTTAAGAG+TGG 0.644286 3:+99119610 MsaT016539.1:CDS
TATAACGGATGGATGGGGCA+TGG 0.644822 3:+99120206 MsaT016539.1:CDS
TGGCAGCTGGAAGTATGCAA+AGG 0.646432 3:-99120701 None:intergenic
CAAAAGAATGCTGATGCAAG+TGG 0.647309 3:+99121133 MsaT016539.1:CDS
GGCTGATCCTGATAGAGTCG+AGG 0.649107 3:+99120077 MsaT016539.1:CDS
CACCTGGTAGCAAGCAAATG+CGG 0.651152 3:+99116447 MsaT016539.1:CDS
CCAACAGCAAAATCTCTGCA+CGG 0.656607 3:-99119004 None:intergenic
AAGGAACATGATGTAAGCCA+GGG 0.656718 3:+99120434 MsaT016539.1:CDS
AGGGACCATTCTCTTAAGAG+TGG 0.657090 3:+99119340 MsaT016539.1:CDS
AGGGACCATTCTCTTAAGAG+TGG 0.657090 3:+99119430 MsaT016539.1:CDS
CCCACCTGAAACCACGAGGA+GGG 0.658576 3:-99118931 None:intergenic
AGGGACCATTCTCCAAAGAG+TGG 0.659573 3:+99119520 MsaT016539.1:CDS
TTTGCAGCCTGGTCAAAACA+CGG 0.660051 3:+99121297 MsaT016539.1:CDS
AACCCAGGAAGAGAAAACTT+GGG 0.660535 3:+99121220 MsaT016539.1:CDS
TGACTGGGAACCATGCATGT+TGG 0.671378 3:-99120315 None:intergenic
TCCCACCTGAAACCACGAGG+AGG 0.671552 3:-99118932 None:intergenic
TGGCTAGATATGTACCACCC+TGG 0.672330 3:-99120451 None:intergenic
TTTAAAGCAGCATATAGCTG+CGG 0.673413 3:-99120974 None:intergenic
GCTACCAGCAACATCTGCAG+AGG 0.681868 3:-99116480 None:intergenic
TTCCCAGTCAATAGGTACAA+CGG 0.683573 3:+99120328 MsaT016539.1:CDS
ACTCTTGACATGTTCTGAGG+AGG 0.684272 3:-99121260 None:intergenic
TAGTTGGGAAGGTAGGCACA+GGG 0.685040 3:+99119087 MsaT016539.1:CDS
ACTAGAGAAAGGGAAAATGG+AGG 0.685470 3:+99119469 MsaT016539.1:CDS
GAACATGATGTAAGCCAGGG+TGG 0.694356 3:+99120437 MsaT016539.1:CDS
TCCAAGGATATGAGCCAGAG+AGG 0.701534 3:-99120936 None:intergenic
TCTACTGAGCAAAAGTACAC+CGG 0.702328 3:+99120806 MsaT016539.1:CDS
CGGAGTCGGAGCCCTCCTCG+TGG 0.703718 3:+99118920 MsaT016539.1:CDS
GTGGGGTTCAGATAGTGACA+TGG 0.704274 3:+99120056 MsaT016539.1:CDS
AGGCTAAGAGATGATAGGTG+GGG 0.711837 3:+99120039 MsaT016539.1:CDS
GAATCCCACCTGAAACCACG+AGG 0.757642 3:-99118935 None:intergenic
AGTAACGGGTACCATCCAGG+AGG 0.795570 3:+99121585 MsaT016539.1:CDS
GCAGCCTGGTCAAAACACGG+TGG 0.812953 3:+99121300 MsaT016539.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 99116386 99122146 99116386 ID=MsaG016539
Chr3 mRNA 99116386 99122146 99116386 ID=MsaT016539.1;Parent=MsaG016539
Chr3 exon 99116386 99116720 99116386 ID=MsaT016539.1.exon1;Parent=MsaT016539.1
Chr3 CDS 99116386 99116720 99116386 ID=cds.MsaT016539.1;Parent=MsaT016539.1
Chr3 exon 99118907 99120227 99118907 ID=MsaT016539.1.exon2;Parent=MsaT016539.1
Chr3 CDS 99118907 99120227 99118907 ID=cds.MsaT016539.1;Parent=MsaT016539.1
Chr3 exon 99120278 99120506 99120278 ID=MsaT016539.1.exon3;Parent=MsaT016539.1
Chr3 CDS 99120278 99120506 99120278 ID=cds.MsaT016539.1;Parent=MsaT016539.1
Chr3 exon 99120666 99121684 99120666 ID=MsaT016539.1.exon4;Parent=MsaT016539.1
Chr3 CDS 99120666 99121684 99120666 ID=cds.MsaT016539.1;Parent=MsaT016539.1
Chr3 exon 99122117 99122146 99122117 ID=MsaT016539.1.exon5;Parent=MsaT016539.1
Chr3 CDS 99122117 99122146 99122117 ID=cds.MsaT016539.1;Parent=MsaT016539.1
Gene Sequence

>MsaT016539.1

ATGAGTGGAGCTGGCAGAAAGCACTCTTCAAAGTGGGATTTGAGTGATGAGCATAAATTCTCACCTGGTAGCAAGCAAATGCGGTCTGGACGGTCCTCTGCAGATGTTGCTGGTAGCAATAGTTCAGAATGGGCTTATTTGGAAGGAAATGATAAATTAAGACCTGTTACGGGATTTTCATCAAAGGAATCTTATTATGGAGGCAGAGGTTCAAATGAGAACGATGCTATGAATAAAGACCATAGAGTTTTTAATTCAAGAAGAGAATGGGACACTGATGGAAGTTACAGCAGGAACATGAGACACAGTCAGTCCCCTAAAAATGACTGGAGCAGGAACAGTCGGAGCCGGAGTCGGAGCCCTCCTCGTGGTTTCAGGTGGGATTCAGGAGTTGATGGTAGAAAAAGGACGAGGGTTGGAGGATCAACACGACCGTGCAGAGATTTTGCTGTTGGAAAATGTAGAAGAGGTAGTCATTGCAATTTTCTGCATCATGATAACCAAAATCGTGAGAATAGTTGGGAAGGTAGGCACAGGGAAGATGGAGCTCCCAGATACTCTGCTACCCATGAGAGTGTTGACCATTCTCTTAAGAGTGGTAGATCTAATGAAGCTTGTATAAATTTTGCAAAAGGCAGCTGTAGAATGGGAGCATCTTGCAAGTTTGTGCATGATTACGATTCTGATGGATATGGTAAAGTTTCTATGGATGAGTTCACTAGAGAAAGGGAAGATGAAGCTCTCAGATATCCTGCTACCCATGAGAGCAGGGACCATTCTCTTAAGAGTGGTAGATCTAATGAATTCATTAGAGAAAGAGAAGATGGAGCTCGCAGATATCCTGCTACCCATGAGAGCAGGGACCATTCTCTTAAGAGTGGTAGATCTAATGAATTCACTAGAGAAAGGGAAAATGGAGGTCCCAGATATTCTGCTACCTATGAGAGCAGGGACCATTCTCCAAAGAGTGGTAGATCTAATGAATTCACTAGTGAAAGGGAAGATGGAGCTCCCAGATATCCTACTACCCATGAGAGCAGGGACTATTCTCTTAAGAGTGGTAGATCTAATGAATTCACTAGTGAAAGGGAAGATGGAGCTCCCAGATATCCTACTAACCACGAGAGCAGGGACTATTCTTTTAAGGGCGGTAGATCTAATGACGAATATGGCAAAGTTTTTATGGATGAATTCACTAGAGAAAGAGAAGTTGGCAGAAGGCACAGAGATAATTCTTTTCAGCAGGGTGGTGGCCGGCATGTGCCTAACCGCACTATTGATGCTCCTTGCAAATTTTTTGCTAGTGGAAATTGTCGAAATGGTAAACATTGTAGGTTTTCTCACGATACGCAAGCATGTAGGAGCCCTATTAGAAGATTAAGGGATGATAGATGGACAAGAAATCCAAGTAGAGATCATCAAATGTTGGATAGACGAAAATTGAGTGACTCCATTAGTCCAAATAAAAGGCTAAGAGATGATAGGTGGGGTTCAGATAGTGACATGGCTGATCCTGATAGAGTCGAGGACAGTCCAAAGCAGAATGATACAGTTTCTGGCTCTGATACAGCAAAGCTGATCGAGAATAAAAGTGGAAATGTAGGTACCACAGAGCCAGAATTTACTGATTTGCCTATAACGGATGGATGGGGCATGAAGGAAGCTGATCTTTGGATAGCAGAAAATACTGGTGCCAACATGCATGGTTCCCAGTCAATAGGTACAACGGACATTTGGCCGGGTGATGCAGAAATGTCTCCTGATTGGAATTATAGGATGGGGTCTTCCAGCCATATGGAAGAACATAAACAGAAGGAACATGATGTAAGCCAGGGTGGTACATATCTAGCCATCTCTGAACACAACAGAATACAACTTGCTCCAGGACAAAACATCAATCAGAATACAGAGAATGTAAATCCTTTGCATACTTCCAGCTGCCATGCAGTTGGACAAAGTCAAGTGGATGTTCCCATTCTTTCTTCAAGAGAAGGCATTGTTGATGCTATACACAGCCAGGAAGTATCTACTGAGCAAAAGTACACCGGAGAACCAAGTATTATGGATTCAGGCTTATCACAAGTTAGTTCAATAAATCCTCCAGTTCAAAACGTAGTAAGCAATGAACAGCTTGCTCAACTCACGAATCTCTCAGCCTCTCTGGCTCATATCCTTGGAGCAGGTCAGCAGCTGCCGCAGCTATATGCTGCTTTAAATTCTCATGAATTGAAGGACAGTCCTTCCCAAGCAAAAATTCAAGTGCCTGCAATGCCTATTTCAATCACATGCATCAAGCCAGATCCTGCCGTTGAACTCCCAAAACAGTATGATCCAATGAATGATAGTATTGAGCAAAAGAATGCTGATGCAAGTGGCGTACCCCCAGCCATTCCTCCAAGTAAAACTATTGCCGAAGTAGAAATTCTGTCGCAGTTGTCTAACCCAGGAAGAGAAAACTTGGGTAATTCAATTAAGGGTGCCTCCTCAGAACATGTCAAGAGTGATAATCTAATCCATTTGCAGCCTGGTCAAAACACGGTGGTCAATAAGGATAACAATGAAGAGGTAGCTAAGGAAAGGAAAAATTCTCAAGATGGTCATAAAAGTACCAAAGAGAATGGTCCTCAAAACATGGACCAAAATGCTGGACCTGATGATGCCAAGCAAACAAAGGAAATGAAAGGATCACGTGCATTTAAATTTGCATTGGCTGAGTTTGTTAAGGAGCTTCTGAAACCAACTTGGAAAGAAGGTAAAATCACTAAAGAAGATTATAAAACGATTGTGAAGAAAGTTGTTGATAAAGTAACGGGTACCATCCAGGAGGCCAATATTCCGCAGACACAAGAAAAAATTGACCAATATTTGTCATTTTCAAAACCAAAGCTTAACAAACTTGTACAGGGAAAAGGCTTTGCTGCTGCTGTTTGCTAA
Protein sequence

>MsaT016539.1

MSGAGRKHSSKWDLSDEHKFSPGSKQMRSGRSSADVAGSNSSEWAYLEGNDKLRPVTGFSSKESYYGGRGSNENDAMNKDHRVFNSRREWDTDGSYSRNMRHSQSPKNDWSRNSRSRSRSPPRGFRWDSGVDGRKRTRVGGSTRPCRDFAVGKCRRGSHCNFLHHDNQNRENSWEGRHREDGAPRYSATHESVDHSLKSGRSNEACINFAKGSCRMGASCKFVHDYDSDGYGKVSMDEFTREREDEALRYPATHESRDHSLKSGRSNEFIREREDGARRYPATHESRDHSLKSGRSNEFTRERENGGPRYSATYESRDHSPKSGRSNEFTSEREDGAPRYPTTHESRDYSLKSGRSNEFTSEREDGAPRYPTNHESRDYSFKGGRSNDEYGKVFMDEFTREREVGRRHRDNSFQQGGGRHVPNRTIDAPCKFFASGNCRNGKHCRFSHDTQACRSPIRRLRDDRWTRNPSRDHQMLDRRKLSDSISPNKRLRDDRWGSDSDMADPDRVEDSPKQNDTVSGSDTAKLIENKSGNVGTTEPEFTDLPITDGWGMKEADLWIAENTGANMHGSQSIGTTDIWPGDAEMSPDWNYRMGSSSHMEEHKQKEHDVSQGGTYLAISEHNRIQLAPGQNINQNTENVNPLHTSSCHAVGQSQVDVPILSSREGIVDAIHSQEVSTEQKYTGEPSIMDSGLSQVSSINPPVQNVVSNEQLAQLTNLSASLAHILGAGQQLPQLYAALNSHELKDSPSQAKIQVPAMPISITCIKPDPAVELPKQYDPMNDSIEQKNADASGVPPAIPPSKTIAEVEILSQLSNPGRENLGNSIKGASSEHVKSDNLIHLQPGQNTVVNKDNNEEVAKERKNSQDGHKSTKENGPQNMDQNAGPDDAKQTKEMKGSRAFKFALAEFVKELLKPTWKEGKITKEDYKTIVKKVVDKVTGTIQEANIPQTQEKIDQYLSFSKPKLNKLVQGKGFAAAVC*