AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016549

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016549 MtrunA17_Chr3g0130101 89.085 284 31 0 4 287 36 319 0.0 521
MsaG016549 MtrunA17_Chr8g0364791 62.778 180 62 2 24 203 55 229 6.34e-77 241
MsaG016549 MtrunA17_Chr8g0384201 61.667 180 64 2 24 203 55 229 8.73e-77 242
MsaG016549 MtrunA17_Chr1g0197361 54.110 146 62 2 24 169 58 198 3.76e-48 169
MsaG016549 MtrunA17_Chr3g0092671 56.693 127 54 1 24 150 55 180 7.38e-47 164
MsaG016549 MtrunA17_Chr1g0201021 46.897 145 72 1 23 162 67 211 2.84e-42 152
MsaG016549 MtrunA17_Chr6g0481091 57.143 105 45 0 23 127 54 158 1.09e-38 136
MsaG016549 MtrunA17_Chr1g0197351 53.077 130 54 2 23 146 90 218 4.36e-38 140
MsaG016549 MtrunA17_Chr5g0433781 49.138 116 58 1 23 137 54 169 1.09e-33 129
MsaG016549 MtrunA17_Chr3g0109821 51.282 117 53 3 23 136 54 169 1.60e-33 129
MsaG016549 MtrunA17_Chr4g0060931 54.206 107 47 1 27 131 67 173 3.62e-33 124
MsaG016549 MtrunA17_Chr5g0428991 36.190 210 123 5 24 227 74 278 6.70e-33 127
MsaG016549 MtrunA17_Chr3g0128151 41.615 161 74 3 24 177 71 218 9.61e-33 126
MsaG016549 MtrunA17_Chr3g0091921 54.206 107 47 1 27 131 66 172 3.84e-32 120
MsaG016549 MtrunA17_Chr2g0309331 55.208 96 40 2 32 126 68 161 1.82e-31 119
MsaG016549 MtrunA17_Chr6g0484441 54.639 97 42 1 29 125 57 151 3.03e-31 119
MsaG016549 MtrunA17_Chr1g0200561 49.515 103 48 2 32 133 77 176 5.48e-31 118
MsaG016549 MtrunA17_Chr4g0018391 54.737 95 39 2 32 125 65 156 5.57e-31 118
MsaG016549 MtrunA17_Chr2g0309891 50.962 104 50 1 23 125 54 157 5.63e-31 118
MsaG016549 MtrunA17_Chr7g0217721 43.077 130 58 3 25 138 67 196 1.07e-30 117
MsaG016549 MtrunA17_Chr8g0372121 40.789 152 78 5 32 182 64 204 2.69e-30 115
MsaG016549 MtrunA17_Chr3g0113001 44.167 120 64 1 25 141 13 132 3.51e-30 115
MsaG016549 MtrunA17_Chr3g0139331 49.533 107 50 2 27 131 61 165 6.54e-30 115
MsaG016549 MtrunA17_Chr2g0316971 54.630 108 43 3 27 129 73 179 7.31e-30 116
MsaG016549 MtrunA17_Chr7g0253011 52.830 106 42 3 27 125 67 171 9.14e-30 115
MsaG016549 MtrunA17_Chr6g0453451 46.154 117 57 2 29 141 72 186 1.07e-29 114
MsaG016549 MtrunA17_Chr3g0128111 47.059 102 54 0 24 125 91 192 1.50e-29 112
MsaG016549 MtrunA17_Chr4g0056541 54.255 94 39 2 33 125 66 156 3.83e-29 114
MsaG016549 MtrunA17_Chr5g0405211 51.579 95 42 2 32 125 66 157 4.68e-29 113
MsaG016549 MtrunA17_Chr2g0312321 52.941 102 39 2 29 125 61 158 5.65e-29 112
MsaG016549 MtrunA17_Chr4g0017921 50.526 95 44 1 32 126 66 157 1.03e-28 112
MsaG016549 MtrunA17_Chr3g0124761 49.020 102 48 2 29 127 59 159 4.33e-28 109
MsaG016549 MtrunA17_Chr2g0321741 48.000 100 44 2 23 122 40 131 1.21e-27 109
MsaG016549 MtrunA17_Chr5g0400461 43.902 123 59 2 23 138 54 173 2.91e-27 108
MsaG016549 MtrunA17_Chr2g0318081 46.392 97 52 0 29 125 68 164 4.55e-27 108
MsaG016549 MtrunA17_Chr7g0217711 51.136 88 43 0 25 112 67 154 5.44e-27 103
MsaG016549 MtrunA17_Chr1g0147421 49.505 101 47 2 27 125 78 176 6.49e-27 107
MsaG016549 MtrunA17_Chr6g0461661 49.038 104 47 3 27 125 68 170 1.43e-26 107
MsaG016549 MtrunA17_Chr8g0346001 45.631 103 47 1 33 126 74 176 2.06e-26 107
MsaG016549 MtrunA17_Chr3g0113031 45.631 103 51 2 41 138 4 106 2.66e-26 104
MsaG016549 MtrunA17_Chr4g0054791 44.643 112 59 1 23 131 54 165 2.88e-26 105
MsaG016549 MtrunA17_Chr1g0183741 50.000 96 42 2 32 125 65 156 4.97e-26 103
MsaG016549 MtrunA17_Chr4g0038751 46.154 104 48 3 25 125 63 161 1.02e-25 103
MsaG016549 MtrunA17_Chr5g0398971 48.454 97 44 4 32 125 65 158 1.04e-25 104
MsaG016549 MtrunA17_Chr8g0384721 44.660 103 52 2 29 127 59 160 2.64e-25 102
MsaG016549 MtrunA17_Chr7g0252971 40.909 110 52 2 29 125 60 169 2.84e-25 103
MsaG016549 MtrunA17_Chr8g0359921 45.455 99 52 1 29 125 66 164 8.07e-25 101
MsaG016549 MtrunA17_Chr7g0261411 49.474 95 42 2 32 124 65 155 8.46e-25 100
MsaG016549 MtrunA17_Chr7g0263931 33.146 178 94 5 29 193 63 228 9.84e-25 101
MsaG016549 MtrunA17_Chr7g0214071 46.429 112 52 4 29 135 62 170 1.06e-24 99.8
MsaG016549 MtrunA17_Chr5g0417791 45.098 102 52 1 29 126 55 156 1.83e-24 99.4
MsaG016549 MtrunA17_Chr3g0130731 46.465 99 49 2 32 127 62 159 3.60e-24 99.0
MsaG016549 MtrunA17_Chr4g0042561 43.137 102 54 2 32 129 64 165 4.83e-24 98.2
MsaG016549 MtrunA17_Chr8g0361731 41.071 112 55 3 25 125 62 173 8.66e-24 99.8
MsaG016549 MtrunA17_Chr6g0453131 41.441 111 55 3 25 125 63 173 2.18e-23 98.6
MsaG016549 MtrunA17_Chr4g0076731 43.810 105 56 2 24 127 101 203 2.64e-23 99.0
MsaG016549 MtrunA17_Chr2g0318101 41.818 110 51 3 29 125 67 176 3.28e-23 97.4
MsaG016549 MtrunA17_Chr3g0144271 45.000 100 46 3 25 122 72 164 5.35e-22 95.1
MsaG016549 MtrunA17_Chr4g0047841 39.344 122 49 3 29 125 67 188 6.38e-22 94.0
MsaG016549 MtrunA17_Chr5g0417001 39.815 108 55 3 28 125 65 172 1.33e-21 93.6
MsaG016549 MtrunA17_Chr8g0385991 40.179 112 54 4 34 137 69 175 2.35e-20 87.4
MsaG016549 MtrunA17_Chr2g0326361 43.119 109 54 4 23 125 62 168 2.53e-20 89.0
MsaG016549 MtrunA17_Chr8g0345671 43.564 101 49 4 24 122 61 155 4.32e-20 89.4
MsaG016549 MtrunA17_Chr2g0321631 40.625 96 53 3 32 123 65 160 8.64e-20 86.3
MsaG016549 MtrunA17_Chr7g0266701 31.902 163 92 6 32 179 65 223 2.48e-19 85.1
MsaG016549 MtrunA17_Chr8g0345691 38.614 101 54 4 24 122 62 156 4.03e-19 86.7
MsaG016549 MtrunA17_Chr4g0026141 37.864 103 60 3 25 123 58 160 6.05e-19 84.3
MsaG016549 MtrunA17_Chr7g0243371 48.750 80 39 1 25 102 12 91 3.15e-18 78.6
MsaG016549 MtrunA17_Chr3g0131911 39.130 115 60 2 29 133 64 178 6.33e-18 81.3
MsaG016549 MtrunA17_Chr8g0345741 38.462 104 56 4 23 124 40 137 1.10e-17 78.2
MsaG016549 MtrunA17_Chr8g0345721 42.529 87 44 3 23 104 113 198 2.04e-17 79.0
MsaG016549 MtrunA17_Chr2g0311651 38.318 107 51 2 34 125 80 186 7.35e-17 79.3
MsaG016549 MtrunA17_Chr5g0442771 35.780 109 58 2 29 125 70 178 7.61e-16 76.3
MsaG016549 MtrunA17_Chr2g0311661 37.383 107 52 3 34 125 79 185 3.22e-15 74.3
MsaG016549 MtrunA17_Chr8g0345711 39.241 79 45 2 24 100 62 139 1.80e-13 67.4
MsaG016549 MtrunA17_Chr8g0345731 40.964 83 43 3 23 100 73 154 6.31e-13 65.9
MsaG016549 MtrunA17_Chr4g0050701 33.981 103 52 3 32 126 66 160 4.85e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016549 AT5G09330.5 43.956 273 118 8 24 273 55 315 1.34e-64 211
MsaG016549 AT5G09330.2 43.956 273 118 8 24 273 55 315 1.34e-64 211
MsaG016549 AT5G09330.4 43.956 273 118 8 24 273 55 315 1.34e-64 211
MsaG016549 AT5G09330.3 43.956 273 118 8 24 273 55 315 1.34e-64 211
MsaG016549 AT5G09330.1 43.956 273 118 8 24 273 55 315 1.34e-64 211
MsaG016549 AT5G64060.1 51.630 184 82 2 23 206 54 230 1.85e-60 196
MsaG016549 AT5G04410.1 61.194 134 51 1 23 156 57 189 3.48e-54 185
MsaG016549 AT3G10500.1 63.559 118 43 0 23 140 57 174 5.58e-50 173
MsaG016549 AT1G34190.1 45.342 161 87 1 23 183 64 223 1.91e-46 164
MsaG016549 AT1G34180.3 49.265 136 68 1 24 158 65 200 2.42e-42 151
MsaG016549 AT1G34180.4 49.265 136 68 1 24 158 77 212 3.19e-42 151
MsaG016549 AT3G10480.3 48.344 151 70 4 2 145 81 230 6.19e-42 151
MsaG016549 AT1G34180.1 49.265 136 68 1 24 158 65 200 1.01e-41 151
MsaG016549 AT1G34180.2 49.265 136 68 1 24 158 77 212 1.31e-41 151
MsaG016549 AT3G10480.2 54.918 122 54 1 24 145 76 196 5.78e-41 147
MsaG016549 AT3G10480.1 55.285 123 53 2 24 145 76 197 8.53e-41 147
MsaG016549 AT3G10490.1 52.756 127 58 2 24 149 76 201 9.68e-41 141
MsaG016549 AT3G10490.2 52.756 127 58 2 24 149 76 201 1.46e-39 144
MsaG016549 AT3G17730.1 57.143 105 45 0 23 127 54 158 8.75e-38 134
MsaG016549 AT1G33060.2 54.902 102 46 0 24 125 73 174 1.09e-36 138
MsaG016549 AT1G33060.1 54.902 102 46 0 24 125 73 174 1.15e-36 138
MsaG016549 AT5G24590.2 43.478 161 73 5 24 183 62 205 6.36e-35 131
MsaG016549 AT5G61430.2 55.046 109 47 1 25 131 1 109 1.57e-34 126
MsaG016549 AT1G65910.1 54.369 103 47 0 23 125 54 156 1.97e-34 132
MsaG016549 AT4G35580.1 54.902 102 46 0 24 125 58 159 7.04e-34 129
MsaG016549 AT4G35580.2 54.902 102 46 0 24 125 58 159 7.66e-34 129
MsaG016549 AT4G35580.3 54.902 102 46 0 24 125 58 159 7.75e-34 129
MsaG016549 AT5G61430.1 55.046 109 47 1 25 131 64 172 8.63e-34 126
MsaG016549 AT5G39610.1 56.481 108 43 2 27 131 70 176 2.47e-33 124
MsaG016549 AT1G32870.2 50.000 120 56 2 23 140 58 175 9.35e-33 126
MsaG016549 AT1G32870.1 50.000 120 56 2 23 140 58 175 1.02e-32 126
MsaG016549 AT1G32870.3 50.000 120 56 2 23 140 94 211 1.64e-32 126
MsaG016549 AT3G49530.2 50.000 104 51 1 24 126 62 165 1.64e-32 124
MsaG016549 AT3G18400.1 55.670 97 42 1 29 125 57 152 1.78e-32 122
MsaG016549 AT3G49530.1 50.000 104 51 1 24 126 62 165 3.55e-32 124
MsaG016549 AT5G07680.2 53.271 107 48 1 27 131 53 159 1.74e-31 119
MsaG016549 AT5G07680.1 53.271 107 48 1 27 131 67 173 2.58e-31 119
MsaG016549 AT1G56010.1 47.863 117 58 1 29 142 5 121 2.79e-31 117
MsaG016549 AT3G12977.2 47.706 109 56 1 22 129 10 118 4.19e-31 116
MsaG016549 AT1G71930.2 49.000 100 49 1 32 131 67 164 4.75e-31 118
MsaG016549 AT1G71930.1 49.000 100 49 1 32 131 67 164 4.75e-31 118
MsaG016549 AT3G03200.1 50.962 104 50 1 23 125 54 157 1.30e-30 120
MsaG016549 AT1G56010.2 47.863 117 58 1 29 142 72 188 1.35e-30 117
MsaG016549 AT5G18270.2 43.636 165 83 5 24 183 68 227 1.36e-30 117
MsaG016549 AT1G12260.2 49.524 105 49 2 33 136 66 167 1.37e-30 117
MsaG016549 AT4G10350.2 50.526 95 47 0 32 126 20 114 1.50e-30 116
MsaG016549 AT5G18270.1 43.827 162 81 5 27 183 71 227 1.64e-30 117
MsaG016549 AT1G12260.1 49.524 105 49 2 33 136 66 167 1.85e-30 118
MsaG016549 AT4G10350.1 41.429 140 68 2 1 126 22 161 1.90e-30 117
MsaG016549 AT1G62700.1 52.083 96 42 2 32 126 65 157 1.98e-30 118
MsaG016549 AT1G62700.2 52.083 96 42 2 32 126 65 157 1.98e-30 118
MsaG016549 AT4G17980.1 51.402 107 48 2 23 125 54 160 3.17e-30 115
MsaG016549 AT5G17260.1 49.038 104 52 1 23 125 54 157 4.02e-30 118
MsaG016549 AT3G12977.1 50.000 104 51 1 27 129 68 171 5.35e-30 114
MsaG016549 AT3G29035.1 50.435 115 55 1 27 139 74 188 5.61e-30 115
MsaG016549 AT4G17980.2 51.402 107 48 2 23 125 54 160 6.11e-30 115
MsaG016549 AT3G10490.4 53.922 102 45 2 49 149 1 101 6.78e-30 116
MsaG016549 AT3G10490.3 53.922 102 45 2 49 149 1 101 6.78e-30 116
MsaG016549 AT2G18060.3 40.278 144 74 3 32 174 67 199 2.30e-29 115
MsaG016549 AT2G18060.1 40.278 144 74 3 32 174 67 199 2.30e-29 115
MsaG016549 AT2G24430.2 52.475 101 45 2 27 125 66 165 6.61e-29 112
MsaG016549 AT2G24430.1 52.475 101 45 2 27 125 66 165 6.61e-29 112
MsaG016549 AT4G36160.1 51.042 96 43 2 32 126 68 160 6.82e-29 114
MsaG016549 AT4G36160.3 51.042 96 43 2 32 126 68 160 6.82e-29 114
MsaG016549 AT4G36160.2 51.042 96 43 2 32 126 72 164 6.93e-29 114
MsaG016549 AT2G27300.1 50.000 100 42 2 23 122 62 153 1.24e-28 112
MsaG016549 AT5G62380.2 52.083 96 42 2 32 126 65 157 1.56e-28 112
MsaG016549 AT5G62380.1 52.083 96 42 2 32 126 65 157 1.56e-28 112
MsaG016549 AT1G54330.1 50.000 110 52 1 23 132 54 160 1.57e-28 111
MsaG016549 AT1G79580.3 50.000 96 44 2 32 126 75 167 1.68e-28 112
MsaG016549 AT1G79580.5 50.000 96 44 2 32 126 75 167 1.68e-28 112
MsaG016549 AT1G79580.4 50.000 96 44 2 32 126 75 167 1.68e-28 112
MsaG016549 AT1G79580.2 50.000 96 44 2 32 126 75 167 1.68e-28 112
MsaG016549 AT1G79580.1 50.000 96 44 2 32 126 75 167 1.68e-28 112
MsaG016549 AT3G04060.1 51.402 107 49 2 25 128 68 174 2.42e-28 111
MsaG016549 AT1G33280.1 48.421 95 46 1 32 126 66 157 5.63e-28 110
MsaG016549 AT1G76420.1 50.476 105 49 2 25 127 70 173 6.20e-28 110
MsaG016549 AT5G46590.1 49.533 107 48 3 23 125 54 158 8.54e-28 109
MsaG016549 AT5G22290.1 44.262 122 52 3 23 135 69 183 9.20e-28 110
MsaG016549 AT5G53950.1 53.846 104 42 3 27 125 67 169 1.29e-27 110
MsaG016549 AT3G44290.2 50.000 100 42 2 23 122 62 153 4.12e-27 105
MsaG016549 AT3G15170.1 49.524 105 49 2 27 127 70 174 1.50e-26 106
MsaG016549 AT1G52890.1 44.898 98 54 0 29 126 66 163 2.18e-26 105
MsaG016549 AT3G44290.1 50.000 100 42 2 23 122 62 153 2.45e-26 106
MsaG016549 AT1G01720.1 44.954 109 55 2 29 133 59 166 2.51e-26 105
MsaG016549 AT3G15500.1 43.878 98 55 0 29 126 66 163 3.72e-26 105
MsaG016549 AT5G08790.1 45.192 104 51 2 29 127 59 161 5.80e-26 104
MsaG016549 AT5G46590.2 52.874 87 40 1 23 108 54 140 6.73e-26 103
MsaG016549 AT1G77450.1 45.098 102 52 2 29 127 62 162 6.96e-26 103
MsaG016549 AT4G28530.1 51.042 96 41 2 32 125 81 172 9.00e-26 105
MsaG016549 AT1G32510.1 44.144 111 54 2 23 125 54 164 1.28e-25 103
MsaG016549 AT1G69490.1 35.669 157 85 2 29 169 61 217 1.83e-25 102
MsaG016549 AT4G27410.2 44.330 97 54 0 29 125 66 162 2.70e-25 102
MsaG016549 AT2G46770.1 42.991 107 48 1 33 126 75 181 3.39e-25 103
MsaG016549 AT4G27410.3 44.330 97 54 0 29 125 83 179 5.20e-25 102
MsaG016549 AT5G66300.1 43.636 110 57 3 32 139 70 176 5.23e-25 101
MsaG016549 AT5G13180.1 49.505 101 48 2 34 132 68 167 1.44e-24 99.8
MsaG016549 AT3G61910.1 45.283 106 45 2 33 125 70 175 2.07e-24 100
MsaG016549 AT2G33480.3 49.524 105 49 3 25 126 1 104 4.03e-24 97.4
MsaG016549 AT5G63790.2 44.231 104 52 2 29 127 112 214 5.08e-24 99.8
MsaG016549 AT5G63790.1 44.231 104 52 2 29 127 102 204 5.24e-24 99.4
MsaG016549 AT2G17040.1 43.269 104 51 3 25 125 54 152 5.65e-24 98.6
MsaG016549 AT1G32770.1 44.340 106 47 2 33 127 75 179 8.32e-24 99.8
MsaG016549 AT1G61110.1 45.455 110 47 3 29 125 68 177 2.13e-23 98.2
MsaG016549 AT2G33480.1 53.191 94 42 2 34 126 69 161 2.47e-23 96.7
MsaG016549 AT3G15510.1 38.816 152 69 8 29 166 69 210 2.66e-23 98.2
MsaG016549 AT2G33480.2 50.538 93 44 1 34 126 69 159 5.12e-23 95.9
MsaG016549 AT2G02450.1 45.000 100 52 2 29 127 103 200 8.25e-23 97.1
MsaG016549 AT3G04070.2 41.284 109 52 2 29 125 62 170 1.19e-22 96.3
MsaG016549 AT2G02450.2 45.000 100 52 2 29 127 103 200 1.21e-22 97.1
MsaG016549 AT1G26870.1 41.284 109 53 3 28 125 67 175 8.24e-22 94.7
MsaG016549 AT3G04070.1 32.143 224 132 6 29 245 62 272 1.86e-21 93.2
MsaG016549 AT1G52880.1 43.119 109 50 3 29 125 69 177 4.53e-21 91.7
MsaG016549 AT5G04395.1 40.909 110 59 2 17 123 81 187 6.44e-21 88.2
MsaG016549 AT2G18060.2 36.364 121 65 3 55 174 2 111 1.01e-19 87.0
MsaG016549 AT2G43000.1 39.796 98 58 1 29 125 70 167 1.68e-19 86.3
MsaG016549 AT4G01520.1 40.952 105 50 3 26 125 57 154 6.95e-19 85.1
MsaG016549 AT4G01540.2 41.905 105 49 3 26 125 57 154 8.17e-19 86.3
MsaG016549 AT4G01540.1 41.905 105 49 3 26 125 57 154 8.40e-19 86.7
MsaG016549 AT4G01540.4 41.905 105 49 3 26 125 57 154 8.48e-19 85.9
MsaG016549 AT4G01540.3 41.905 105 49 3 26 125 57 154 8.48e-19 86.3
MsaG016549 AT5G39820.1 36.290 124 56 4 25 125 68 191 1.21e-18 85.1
MsaG016549 AT5G22380.1 39.394 99 57 2 32 127 66 164 2.50e-18 82.4
MsaG016549 AT5G22290.2 42.222 90 37 2 55 135 1 84 3.95e-18 82.0
MsaG016549 AT3G44350.2 39.796 98 57 2 32 127 65 162 9.66e-18 80.9
MsaG016549 AT4G01550.1 40.000 125 57 6 24 139 54 169 8.60e-17 80.5
MsaG016549 AT2G43000.2 39.080 87 52 1 40 125 3 89 8.14e-16 74.7
MsaG016549 AT3G12910.1 36.449 107 53 2 34 125 79 185 2.31e-15 75.1
MsaG016549 AT3G44350.1 38.542 96 57 2 32 125 65 160 2.93e-15 72.4
MsaG016549 AT3G04420.2 36.893 103 60 1 23 125 53 150 4.33e-14 71.6
MsaG016549 AT3G04420.1 36.893 103 60 1 23 125 53 150 7.78e-14 71.2
MsaG016549 AT1G02230.1 39.048 105 50 4 23 122 53 148 3.28e-13 69.7
MsaG016549 AT1G02220.1 34.286 105 53 3 23 123 53 145 1.62e-12 67.8

Find 90 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCAGTCTTTCTCATTAAA+TGG 0.189856 3:-99221520 None:intergenic
TGTCATTTACTTCAGAAATA+TGG 0.230925 3:-99221624 None:intergenic
TCCATACTGTGCACCATTCC+TGG 0.233076 3:-99221494 None:intergenic
ATCTCATTTGGAATTAAATA+AGG 0.245537 3:-99223564 None:intergenic
GAATTTGATAATGCAATGTT+AGG 0.257176 3:+99219295 None:intergenic
CAATCAGTTCGGTCTCCTTT+AGG 0.274643 3:-99220179 None:intergenic
GTGCAATATTTCTTTCCAAC+AGG 0.361824 3:-99220029 None:intergenic
GGTGCAGACACAGCAGCAAC+AGG 0.376155 3:-99221577 None:intergenic
CCCAGGAATGGTGCACAGTA+TGG 0.387270 3:+99221493 MsaT016549.1:CDS
AGAACTCCTATGTGATTTGT+AGG 0.387741 3:+99221446 MsaT016549.1:intron
AACCATTTAATGAGAAAGAC+TGG 0.390374 3:+99221518 MsaT016549.1:CDS
CCCTCTGCAAGTGGATGTAT+TGG 0.400344 3:+99221652 MsaT016549.1:CDS
GCACGGGAGGGAGGATGAAT+CGG 0.411671 3:+99220048 MsaT016549.1:CDS
TATTAACAGCTTGGTTGGAT+TGG 0.414108 3:+99223605 MsaT016549.1:CDS
CCATACTGTGCACCATTCCT+GGG 0.415740 3:-99221493 None:intergenic
GGAGATATTAACAGCTTGGT+TGG 0.445872 3:+99223600 MsaT016549.1:CDS
GACGATGAAAATGCATTGGC+CGG 0.464243 3:+99221784 MsaT016549.1:CDS
ATGAATCGGGCAACAGAAGT+AGG 0.466475 3:+99220062 MsaT016549.1:CDS
CTAAAGGAGACCGAACTGAT+TGG 0.467195 3:+99220180 MsaT016549.1:CDS
TGGTACTTCTTTACCCCTGT+TGG 0.468244 3:+99220014 MsaT016549.1:CDS
AAAAGGAAGGTCCTGGTCCC+AGG 0.470057 3:+99221476 MsaT016549.1:CDS
TAAAGGAGACCGAACTGATT+GGG 0.479184 3:+99220181 MsaT016549.1:CDS
CACGGGAGGGAGGATGAATC+GGG 0.479266 3:+99220049 MsaT016549.1:CDS
AATTTGATAATGCAATGTTA+GGG 0.487747 3:+99219296 None:intergenic
CGACAGAAGGTTGATAATCC+TGG 0.495670 3:+99223516 MsaT016549.1:intron
ACGACACACCCTTCAGCTCT+AGG 0.498439 3:+99221718 MsaT016549.1:CDS
TCAGCGTTGTTTGCTTGACC+AGG 0.500954 3:-99223534 None:intergenic
CTATCAAGATTGATGCCCTC+TGG 0.507191 3:+99221694 MsaT016549.1:CDS
CACACTGGCAAAGCTCCTAA+AGG 0.508822 3:+99220164 MsaT016549.1:CDS
GGGCAACAGAAGTAGGGTAC+TGG 0.511584 3:+99220069 MsaT016549.1:CDS
GTAGGGTACTGGAAGACTAC+AGG 0.511858 3:+99220080 MsaT016549.1:CDS
GCTTGATTGCCTAGAGCTGA+AGG 0.517653 3:-99221727 None:intergenic
CTTTGCCAGTGTGAAACACC+AGG 0.519578 3:-99220154 None:intergenic
CCTGGGAGATATTAACAGCT+TGG 0.523071 3:+99223596 MsaT016549.1:CDS
GTGGAACACCATTGTCAGCT+AGG 0.523981 3:-99220235 None:intergenic
GATTTGTAGGGTATTTCAAA+AGG 0.527496 3:+99221459 MsaT016549.1:CDS
AGCTTGGTTGGATTGGATGA+TGG 0.527918 3:+99223612 MsaT016549.1:CDS
TGTAGGGTATTTCAAAAGGA+AGG 0.527921 3:+99221463 MsaT016549.1:CDS
GCTACAACGGATGGTGATGA+TGG 0.530888 3:-99219356 None:intergenic
TCCAATACATCCACTTGCAG+AGG 0.532823 3:-99221653 None:intergenic
TTTCGACGATGAAAATGCAT+TGG 0.532966 3:+99221780 MsaT016549.1:CDS
ATCGTCGAAAATAGCAAGCA+TGG 0.533664 3:-99221767 None:intergenic
CATGCATAACCCAATCAGTT+CGG 0.542397 3:-99220190 None:intergenic
AGCTGACAATGGTGTTCCAC+AGG 0.546378 3:+99220238 MsaT016549.1:CDS
AGAAATATTGCACGGGAGGG+AGG 0.549227 3:+99220039 MsaT016549.1:CDS
TCTTGATAGACATGATAAAG+AGG 0.553266 3:-99221680 None:intergenic
GAACATAAGAATCAAGTGGT+GGG 0.560868 3:+99220119 MsaT016549.1:CDS
TGCAATATTTCTTTCCAACA+GGG 0.561122 3:-99220028 None:intergenic
GAAGGTCCTGGTCCCAGGAA+TGG 0.561485 3:+99221481 MsaT016549.1:CDS
GACTGGAAAGCTACAACGGA+TGG 0.563593 3:-99219365 None:intergenic
TCAGAAATATGGCTTGAGCT+AGG 0.568979 3:-99221613 None:intergenic
GATAAATCTTTGCTCAAGAG+TGG 0.570392 3:+99219981 MsaT016549.1:intron
GTTGGATTGGATGATGGAAG+CGG 0.572350 3:+99223618 MsaT016549.1:CDS
GGAAGACTACAGGGAAGGAT+AGG 0.572952 3:+99220090 MsaT016549.1:CDS
ACGATGAAAATGCATTGGCC+GGG 0.583391 3:+99221785 MsaT016549.1:CDS
GTATTTCAAAAGGAAGGTCC+TGG 0.583445 3:+99221469 MsaT016549.1:CDS
GAACTCCTATGTGATTTGTA+GGG 0.583857 3:+99221447 MsaT016549.1:intron
TGAAGGGTGTGTCGTGCCAG+AGG 0.584025 3:-99221710 None:intergenic
AACATAAGAATCAAGTGGTG+GGG 0.590194 3:+99220120 MsaT016549.1:CDS
GAAGGGTGTGTCGTGCCAGA+GGG 0.592236 3:-99221709 None:intergenic
TGAATCGGGCAACAGAAGTA+GGG 0.592463 3:+99220063 MsaT016549.1:CDS
GGTCAAGCAAACAACGCTGA+AGG 0.593547 3:+99223537 MsaT016549.1:CDS
GGAAAGAAATATTGCACGGG+AGG 0.596853 3:+99220035 MsaT016549.1:CDS
GACATGCATCCCTCTGCAAG+TGG 0.596887 3:+99221643 MsaT016549.1:CDS
AGAACCCTGGTGTTTCACAC+TGG 0.601634 3:+99220149 MsaT016549.1:CDS
TGTGCACCATTCCTGGGACC+AGG 0.603992 3:-99221487 None:intergenic
CCAAGCTGTTAATATCTCCC+AGG 0.614179 3:-99223596 None:intergenic
CTTGATTGCCTAGAGCTGAA+GGG 0.615214 3:-99221726 None:intergenic
GAATGAAAACAATGGATCTG+AGG 0.618267 3:+99221807 MsaT016549.1:CDS
GCAATATTTCTTTCCAACAG+GGG 0.618582 3:-99220027 None:intergenic
CCAATACATCCACTTGCAGA+GGG 0.625389 3:-99221652 None:intergenic
GAGCTAGGTAGAATGAGAGC+TGG 0.627457 3:-99221598 None:intergenic
GTTGGAAAGAAATATTGCAC+GGG 0.629534 3:+99220032 MsaT016549.1:CDS
TGAACATAAGAATCAAGTGG+TGG 0.630537 3:+99220118 MsaT016549.1:CDS
TGTTGGAAAGAAATATTGCA+CGG 0.632190 3:+99220031 MsaT016549.1:CDS
ACCATTTAATGAGAAAGACT+GGG 0.636505 3:+99221519 MsaT016549.1:CDS
TAGGGTACTGGAAGACTACA+GGG 0.648401 3:+99220081 MsaT016549.1:CDS
TTGGCCGGGAATGAAAACAA+TGG 0.650090 3:+99221799 MsaT016549.1:CDS
GACAAAGACCTAGCTGACAA+TGG 0.652509 3:+99220227 MsaT016549.1:CDS
GTGCAGACACAGCAGCAACA+GGG 0.657913 3:-99221576 None:intergenic
AAATACCCTACAAATCACAT+AGG 0.659266 3:-99221452 None:intergenic
TCAAGAGTGGTGATTTGCAA+TGG 0.660182 3:+99219994 MsaT016549.1:CDS
GTGATGATAAGAAAAGTCAT+CGG 0.671182 3:-99219329 None:intergenic
GAAAGAAATATTGCACGGGA+GGG 0.676449 3:+99220036 MsaT016549.1:CDS
GATTGAACATAAGAATCAAG+TGG 0.676975 3:+99220115 MsaT016549.1:CDS
GTACTGGAAGACTACAGGGA+AGG 0.680520 3:+99220085 MsaT016549.1:CDS
AAGAAAAGTCATCGGCACAG+TGG 0.690558 3:-99219321 None:intergenic
GAAAGACTGGGATAGCGAAG+AGG 0.695888 3:+99221531 MsaT016549.1:CDS
TTTGCCAGTGTGAAACACCA+GGG 0.703208 3:-99220153 None:intergenic
GGTGGGGATGATAAGAACCC+TGG 0.707991 3:+99220136 MsaT016549.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 99219310 99223674 99219310 ID=MsaG016549
Chr3 mRNA 99219310 99223674 99219310 ID=MsaT016549.1;Parent=MsaG016549
Chr3 exon 99219310 99219373 99219310 ID=MsaT016549.1.exon1;Parent=MsaT016549.1
Chr3 CDS 99219310 99219373 99219310 ID=cds.MsaT016549.1;Parent=MsaT016549.1
Chr3 exon 99219982 99220259 99219982 ID=MsaT016549.1.exon2;Parent=MsaT016549.1
Chr3 CDS 99219982 99220259 99219982 ID=cds.MsaT016549.1;Parent=MsaT016549.1
Chr3 exon 99221448 99221828 99221448 ID=MsaT016549.1.exon3;Parent=MsaT016549.1
Chr3 CDS 99221448 99221828 99221448 ID=cds.MsaT016549.1;Parent=MsaT016549.1
Chr3 exon 99223522 99223674 99223522 ID=MsaT016549.1.exon4;Parent=MsaT016549.1
Chr3 CDS 99223522 99223674 99223522 ID=cds.MsaT016549.1;Parent=MsaT016549.1
Gene Sequence

>MsaT016549.1

ATGTTAGGGTTCCACTGTGCCGATGACTTTTCTTATCATCACGGAACCATCATCACCATCCGTTATAAATCTTTGCTCAAGAGTGGTGATTTGCAATGGTACTTCTTTACCCCTGTTGGAAAGAAATATTGCACGGGAGGGAGGATGAATCGGGCAACAGAAGTAGGGTACTGGAAGACTACAGGGAAGGATAGGTCGATTGAACATAAGAATCAAGTGGTGGGGATGATAAGAACCCTGGTGTTTCACACTGGCAAAGCTCCTAAAGGAGACCGAACTGATTGGGTTATGCATGAATACAGACTTGAAGACAAAGACCTAGCTGACAATGGTGTTCCACAGAACTCCTATGTGATTTGTAGGGTATTTCAAAAGGAAGGTCCTGGTCCCAGGAATGGTGCACAGTATGGAAAACCATTTAATGAGAAAGACTGGGATAGCGAAGAGGAAATTGATTATGTACAAGCTGTCCCTGTTGCTGCTGTGTCTGCACCAGCTCTCATTCTACCTAGCTCAAGCCATATTTCTGAAGTAAATGACATGCATCCCTCTGCAAGTGGATGTATTGGACTGACCTCTTTATCATGTCTATCAAGATTGATGCCCTCTGGCACGACACACCCTTCAGCTCTAGGCAATCAAGCTGATGATGACATTTTATCCATGCTTGCTATTTTCGACGATGAAAATGCATTGGCCGGGAATGAAAACAATGGATCTGAGAAGGTTGATAATCCTGGTCAAGCAAACAACGCTGAAGGTGCACCTTATTTAATTCCAAATGAGATTTTTGAGGACCTGGGAGATATTAACAGCTTGGTTGGATTGGATGATGGAAGCGGTTTTTCCTATGGCCAAAAGATGAAAATACTATAA
Protein sequence

>MsaT016549.1

MLGFHCADDFSYHHGTIITIRYKSLLKSGDLQWYFFTPVGKKYCTGGRMNRATEVGYWKTTGKDRSIEHKNQVVGMIRTLVFHTGKAPKGDRTDWVMHEYRLEDKDLADNGVPQNSYVICRVFQKEGPGPRNGAQYGKPFNEKDWDSEEEIDYVQAVPVAAVSAPALILPSSSHISEVNDMHPSASGCIGLTSLSCLSRLMPSGTTHPSALGNQADDDILSMLAIFDDENALAGNENNGSEKVDNPGQANNAEGAPYLIPNEIFEDLGDINSLVGLDDGSGFSYGQKMKIL*