AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016569

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016569 MtrunA17_Chr3g0130321 94.712 208 9 2 1 206 1 208 5.24e-143 397
MsaG016569 MtrunA17_Chr8g0384351 57.843 204 58 9 5 206 8 185 3.82e-65 199
MsaG016569 MtrunA17_Chr4g0050701 42.500 200 94 6 7 205 11 190 1.29e-46 152
MsaG016569 MtrunA17_Chr5g0417791 32.864 213 119 7 5 201 1 205 6.53e-30 111
MsaG016569 MtrunA17_Chr4g0042561 37.888 161 84 6 6 158 8 160 2.39e-27 104
MsaG016569 MtrunA17_Chr2g0318101 36.810 163 90 5 7 158 15 175 4.07e-27 105
MsaG016569 MtrunA17_Chr7g0263931 36.810 163 87 6 5 158 9 164 5.82e-26 102
MsaG016569 MtrunA17_Chr4g0060931 40.373 161 76 7 7 158 17 166 1.04e-25 102
MsaG016569 MtrunA17_Chr4g0047841 34.286 175 90 5 7 158 15 187 2.52e-25 100
MsaG016569 MtrunA17_Chr3g0091921 37.654 162 87 4 3 158 12 165 3.59e-25 99.8
MsaG016569 MtrunA17_Chr3g0139331 40.373 161 74 6 7 158 11 158 1.44e-24 98.6
MsaG016569 MtrunA17_Chr1g0147421 40.606 165 69 8 7 158 27 175 3.47e-24 97.8
MsaG016569 MtrunA17_Chr2g0318081 33.125 160 91 3 5 158 14 163 3.95e-24 97.8
MsaG016569 MtrunA17_Chr2g0326361 36.420 162 81 7 7 156 14 165 4.59e-24 97.1
MsaG016569 MtrunA17_Chr3g0131911 32.515 163 96 4 4 157 11 168 7.44e-24 94.7
MsaG016569 MtrunA17_Chr2g0309331 32.317 164 89 5 5 158 8 159 1.09e-23 96.3
MsaG016569 MtrunA17_Chr3g0124761 32.515 163 96 4 1 157 1 155 1.23e-23 95.1
MsaG016569 MtrunA17_Chr8g0384721 32.121 165 95 4 1 157 1 156 2.04e-23 94.7
MsaG016569 MtrunA17_Chr7g0214071 35.185 162 80 5 5 157 13 158 4.48e-23 93.2
MsaG016569 MtrunA17_Chr6g0484441 37.500 160 78 6 7 158 5 150 1.12e-22 93.2
MsaG016569 MtrunA17_Chr3g0109821 32.022 178 104 4 5 174 4 172 1.37e-22 95.1
MsaG016569 MtrunA17_Chr8g0359921 32.022 178 105 4 5 176 12 179 1.52e-22 93.2
MsaG016569 MtrunA17_Chr8g0385991 30.625 160 98 4 4 156 11 164 6.22e-22 89.4
MsaG016569 MtrunA17_Chr7g0252971 32.370 173 94 7 5 161 6 171 1.27e-21 90.5
MsaG016569 MtrunA17_Chr4g0017921 32.222 180 97 7 7 175 8 173 1.67e-21 90.5
MsaG016569 MtrunA17_Chr2g0316971 36.646 161 84 6 7 158 23 174 2.11e-21 90.5
MsaG016569 MtrunA17_Chr3g0130731 31.447 159 95 4 5 157 5 155 2.98e-21 89.0
MsaG016569 MtrunA17_Chr2g0312321 34.161 161 85 6 5 157 9 156 3.20e-21 89.0
MsaG016569 MtrunA17_Chr6g0481091 36.641 131 74 3 5 127 4 133 4.93e-21 87.4
MsaG016569 MtrunA17_Chr8g0346001 30.247 162 103 3 6 158 14 174 5.05e-21 89.7
MsaG016569 MtrunA17_Chr7g0253011 31.132 212 127 5 7 206 17 221 8.54e-21 89.0
MsaG016569 MtrunA17_Chr5g0433781 32.787 183 105 5 5 179 4 176 1.09e-20 89.4
MsaG016569 MtrunA17_Chr8g0361731 31.214 173 105 5 9 171 16 184 1.35e-20 88.6
MsaG016569 MtrunA17_Chr5g0398971 29.341 167 99 4 1 158 1 157 1.84e-20 87.4
MsaG016569 MtrunA17_Chr1g0183741 29.714 175 98 4 5 167 8 169 4.28e-20 85.5
MsaG016569 MtrunA17_Chr8g0384201 34.146 164 88 5 4 158 3 155 2.01e-19 85.5
MsaG016569 MtrunA17_Chr3g0130101 30.846 201 115 8 1 189 1 189 2.01e-19 84.7
MsaG016569 MtrunA17_Chr4g0056541 31.319 182 102 6 1 173 1 168 2.35e-19 84.7
MsaG016569 MtrunA17_Chr7g0261411 30.675 163 87 4 5 157 8 154 3.78e-19 83.2
MsaG016569 MtrunA17_Chr6g0453131 29.114 158 106 1 7 158 15 172 8.70e-19 83.2
MsaG016569 MtrunA17_Chr2g0309891 29.630 162 97 3 5 158 4 156 1.03e-18 82.8
MsaG016569 MtrunA17_Chr8g0345731 34.000 150 87 4 7 144 24 173 1.48e-18 79.3
MsaG016569 MtrunA17_Chr6g0453451 32.704 159 93 4 6 158 19 169 1.50e-18 82.0
MsaG016569 MtrunA17_Chr4g0018391 30.178 169 93 6 1 158 1 155 1.84e-18 82.0
MsaG016569 MtrunA17_Chr5g0417001 31.214 173 104 6 9 171 16 183 2.09e-18 82.4
MsaG016569 MtrunA17_Chr1g0197351 32.353 170 102 5 7 168 42 206 3.03e-18 82.0
MsaG016569 MtrunA17_Chr1g0200561 29.630 162 91 6 7 158 19 167 4.75e-18 80.9
MsaG016569 MtrunA17_Chr2g0321631 31.395 172 95 7 1 159 1 162 6.81e-18 79.3
MsaG016569 MtrunA17_Chr2g0311651 29.878 164 99 5 9 158 24 185 7.34e-18 80.1
MsaG016569 MtrunA17_Chr5g0400461 27.835 194 114 3 5 184 4 185 9.46e-18 80.1
MsaG016569 MtrunA17_Chr8g0364791 33.742 163 90 5 4 158 3 155 1.01e-17 80.5
MsaG016569 MtrunA17_Chr3g0128151 33.750 160 88 4 7 158 22 171 1.12e-17 80.5
MsaG016569 MtrunA17_Chr7g0217721 28.378 222 131 5 5 205 17 231 1.70e-17 79.3
MsaG016569 MtrunA17_Chr5g0428991 32.919 161 90 5 6 158 24 174 1.77e-17 80.1
MsaG016569 MtrunA17_Chr4g0026141 30.000 170 100 5 1 159 1 162 2.04e-17 78.2
MsaG016569 MtrunA17_Chr2g0311661 29.193 161 100 4 9 156 23 182 2.17e-17 78.6
MsaG016569 MtrunA17_Chr5g0442771 27.673 159 105 4 9 158 20 177 2.82e-17 78.6
MsaG016569 MtrunA17_Chr3g0144271 35.252 139 82 3 7 137 23 161 3.67e-17 79.0
MsaG016569 MtrunA17_Chr8g0345671 33.129 163 78 8 7 156 11 155 4.71e-17 78.6
MsaG016569 MtrunA17_Chr5g0405211 28.221 163 92 6 7 158 8 156 6.80e-17 77.8
MsaG016569 MtrunA17_Chr8g0372121 30.534 131 79 4 7 127 6 134 1.53e-16 76.3
MsaG016569 MtrunA17_Chr1g0197361 30.000 160 90 6 9 158 10 157 2.17e-16 77.0
MsaG016569 MtrunA17_Chr7g0217711 33.333 129 79 2 5 127 17 144 2.54e-16 73.6
MsaG016569 MtrunA17_Chr6g0461661 33.594 128 78 2 7 127 18 145 2.67e-16 76.3
MsaG016569 MtrunA17_Chr4g0054791 30.357 168 96 5 5 162 4 160 5.49e-16 74.7
MsaG016569 MtrunA17_Chr4g0076731 30.128 156 92 3 9 158 56 200 5.54e-16 75.5
MsaG016569 MtrunA17_Chr3g0092671 30.435 161 88 7 9 158 7 154 1.09e-15 74.7
MsaG016569 MtrunA17_Chr3g0128111 31.210 157 90 4 10 158 45 191 2.17e-15 72.4
MsaG016569 MtrunA17_Chr1g0201021 30.709 127 79 2 9 127 21 146 2.48e-13 68.2
MsaG016569 MtrunA17_Chr3g0113031 37.500 96 54 2 76 171 15 104 3.98e-13 66.2
MsaG016569 MtrunA17_Chr7g0266701 24.762 210 130 7 7 195 4 206 5.17e-13 65.9
MsaG016569 MtrunA17_Chr8g0345711 30.769 130 79 4 7 126 12 140 7.95e-13 63.9
MsaG016569 MtrunA17_Chr3g0113001 34.711 121 62 5 60 172 17 128 2.11e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016569 AT5G64530.1 42.995 207 92 6 5 205 1 187 3.53e-44 146
MsaG016569 AT5G64530.2 50.806 124 54 3 5 127 1 118 5.41e-35 121
MsaG016569 AT1G61110.1 36.257 171 94 4 7 165 16 183 3.61e-29 110
MsaG016569 AT2G24430.2 40.000 160 77 5 7 158 16 164 1.56e-26 103
MsaG016569 AT2G24430.1 40.000 160 77 5 7 158 16 164 1.56e-26 103
MsaG016569 AT1G01720.1 36.364 165 88 6 1 157 1 156 5.92e-26 101
MsaG016569 AT5G07680.2 39.623 159 78 6 7 157 3 151 8.00e-26 102
MsaG016569 AT5G07680.1 39.623 159 78 6 7 157 17 165 8.95e-26 102
MsaG016569 AT3G04070.2 35.976 164 89 5 7 158 10 169 5.87e-25 100
MsaG016569 AT3G04060.1 37.805 164 83 6 3 157 16 169 7.39e-25 99.8
MsaG016569 AT1G52890.1 33.750 160 90 3 5 158 12 161 1.91e-24 98.2
MsaG016569 AT1G69490.1 34.591 159 90 5 7 158 9 160 1.96e-24 97.4
MsaG016569 AT3G18400.1 38.750 160 77 5 7 158 5 151 2.87e-24 97.8
MsaG016569 AT3G04070.1 34.270 178 89 6 7 158 10 185 4.40e-24 98.2
MsaG016569 AT5G39610.1 35.366 164 84 4 5 158 18 169 4.60e-24 96.7
MsaG016569 AT1G77450.1 33.951 162 93 4 2 157 5 158 5.90e-24 95.9
MsaG016569 AT5G61430.1 37.107 159 84 5 7 158 16 165 8.05e-24 97.1
MsaG016569 AT4G27410.2 33.125 160 91 4 5 158 12 161 8.94e-24 96.3
MsaG016569 AT5G18270.2 36.196 163 87 5 3 157 17 170 1.61e-23 96.3
MsaG016569 AT5G08790.1 28.155 206 133 4 1 197 1 200 1.79e-23 95.1
MsaG016569 AT5G18270.1 35.583 163 88 5 3 157 17 170 2.75e-23 95.5
MsaG016569 AT3G15500.1 32.500 160 92 3 5 158 12 161 3.76e-23 94.7
MsaG016569 AT5G22290.1 36.029 136 79 3 9 137 23 157 8.91e-23 94.4
MsaG016569 AT5G46590.1 34.177 158 88 3 7 156 6 155 1.02e-22 93.2
MsaG016569 AT5G53950.1 37.349 166 86 6 2 158 12 168 1.80e-22 94.0
MsaG016569 AT3G15510.1 33.962 159 92 5 11 158 21 177 3.93e-22 92.8
MsaG016569 AT4G10350.1 31.000 200 112 7 7 191 9 197 4.42e-22 92.4
MsaG016569 AT3G61910.1 32.571 175 99 6 6 163 10 182 6.99e-22 91.7
MsaG016569 AT1G76420.1 37.423 163 75 7 7 157 22 169 1.34e-21 90.9
MsaG016569 AT3G10500.1 33.333 165 92 4 2 158 4 158 1.39e-21 92.4
MsaG016569 AT5G63790.1 32.164 171 100 5 1 163 44 206 1.49e-21 90.5
MsaG016569 AT5G63790.2 32.164 171 100 5 1 163 54 216 1.76e-21 90.5
MsaG016569 AT1G79580.3 31.138 167 92 5 2 158 12 165 4.17e-21 90.1
MsaG016569 AT1G79580.5 31.138 167 92 5 2 158 12 165 4.17e-21 90.1
MsaG016569 AT1G79580.4 31.138 167 92 5 2 158 12 165 4.17e-21 90.1
MsaG016569 AT1G79580.2 31.138 167 92 5 2 158 12 165 4.17e-21 90.1
MsaG016569 AT1G79580.1 31.138 167 92 5 2 158 12 165 4.17e-21 90.1
MsaG016569 AT3G44290.2 34.848 132 78 3 9 133 16 146 5.25e-21 87.4
MsaG016569 AT5G46590.2 36.154 130 75 2 7 128 6 135 7.06e-21 88.2
MsaG016569 AT3G12977.1 31.098 164 98 4 5 162 16 170 8.16e-21 87.8
MsaG016569 AT2G33480.1 34.783 161 84 6 4 157 12 158 9.47e-21 87.4
MsaG016569 AT5G04410.1 32.727 165 93 4 2 158 4 158 1.06e-20 89.7
MsaG016569 AT5G17260.1 32.716 162 92 3 5 158 4 156 1.07e-20 89.7
MsaG016569 AT4G27410.3 32.203 177 87 6 5 158 12 178 1.18e-20 88.2
MsaG016569 AT3G17730.1 35.878 131 75 3 5 127 4 133 1.52e-20 86.7
MsaG016569 AT2G46770.1 30.814 172 104 6 6 164 15 184 1.55e-20 88.2
MsaG016569 AT3G44290.1 34.848 132 78 3 9 133 16 146 1.94e-20 87.8
MsaG016569 AT5G13180.1 32.927 164 84 6 4 157 11 158 4.65e-20 85.5
MsaG016569 AT3G15170.1 35.669 157 87 4 7 156 20 169 4.97e-20 86.3
MsaG016569 AT1G33280.1 31.138 167 92 5 7 164 8 160 5.34e-20 86.3
MsaG016569 AT3G29035.1 40.458 131 67 4 5 127 22 149 7.34e-20 85.9
MsaG016569 AT4G35580.1 31.633 196 100 6 7 183 9 189 1.27e-19 86.7
MsaG016569 AT2G33480.2 34.161 161 83 6 4 157 12 156 1.33e-19 84.3
MsaG016569 AT4G35580.3 31.633 196 100 6 7 183 9 189 1.78e-19 86.3
MsaG016569 AT4G35580.2 31.633 196 100 6 7 183 9 189 1.80e-19 86.3
MsaG016569 AT1G32510.1 30.488 164 92 3 7 156 6 161 2.67e-19 84.0
MsaG016569 AT1G56010.2 31.677 161 94 5 5 157 17 169 2.75e-19 84.3
MsaG016569 AT1G65910.1 32.716 162 91 4 5 158 4 155 4.45e-19 85.1
MsaG016569 AT3G03200.1 30.864 162 95 3 5 158 4 156 5.28e-19 84.7
MsaG016569 AT1G32770.1 30.120 166 99 5 6 158 15 176 6.03e-19 84.0
MsaG016569 AT2G02450.1 28.421 190 115 3 9 188 53 231 6.81e-19 84.0
MsaG016569 AT1G12260.2 27.650 217 124 8 1 205 1 196 7.56e-19 83.6
MsaG016569 AT2G02450.2 28.421 190 115 3 9 188 53 231 9.86e-19 83.6
MsaG016569 AT5G62380.2 29.825 171 91 7 1 158 1 155 1.18e-18 83.2
MsaG016569 AT5G62380.1 29.825 171 91 7 1 158 1 155 1.18e-18 83.2
MsaG016569 AT1G12260.1 27.650 217 124 8 1 205 1 196 1.45e-18 83.2
MsaG016569 AT2G43000.1 28.205 156 97 3 9 158 20 166 1.86e-18 81.6
MsaG016569 AT5G24590.2 30.061 163 97 4 4 158 10 163 2.36e-18 82.8
MsaG016569 AT4G17980.1 32.727 165 93 5 7 162 6 161 2.66e-18 80.9
MsaG016569 AT1G52880.1 31.138 167 85 6 11 158 21 176 3.19e-18 81.6
MsaG016569 AT4G17980.2 32.704 159 91 4 7 156 6 157 3.51e-18 80.9
MsaG016569 AT1G33060.1 32.704 159 89 4 7 157 24 172 7.03e-18 81.6
MsaG016569 AT1G33060.2 32.704 159 89 4 7 157 24 172 7.32e-18 81.6
MsaG016569 AT2G27300.1 34.351 131 78 3 4 127 11 140 9.77e-18 80.5
MsaG016569 AT2G18060.3 29.012 162 92 6 7 158 9 157 1.17e-17 80.5
MsaG016569 AT2G18060.1 29.012 162 92 6 7 158 9 157 1.17e-17 80.5
MsaG016569 AT4G36160.2 28.395 162 93 5 7 158 14 162 1.99e-17 79.7
MsaG016569 AT4G36160.1 28.395 162 93 5 7 158 10 158 2.21e-17 79.7
MsaG016569 AT4G36160.3 28.395 162 93 5 7 158 10 158 2.21e-17 79.7
MsaG016569 AT4G28530.1 28.571 175 89 4 6 157 9 170 2.62e-17 79.3
MsaG016569 AT1G26870.1 30.247 162 100 4 7 158 16 174 3.62e-17 79.3
MsaG016569 AT5G22380.1 31.361 169 95 5 1 157 1 160 5.23e-17 77.0
MsaG016569 AT1G62700.1 29.167 168 96 6 1 158 1 155 6.93e-17 78.6
MsaG016569 AT1G62700.2 29.167 168 96 6 1 158 1 155 6.93e-17 78.6
MsaG016569 AT5G39820.1 28.177 181 109 6 9 169 22 201 1.39e-16 77.0
MsaG016569 AT1G71930.2 27.647 170 98 7 1 158 1 157 1.50e-16 77.0
MsaG016569 AT1G71930.1 27.647 170 98 7 1 158 1 157 1.50e-16 77.0
MsaG016569 AT1G54330.1 29.143 175 97 4 5 168 4 162 1.96e-16 76.3
MsaG016569 AT3G10480.2 32.759 174 97 6 7 172 27 188 2.10e-16 77.0
MsaG016569 AT3G12910.1 26.036 169 110 6 3 158 18 184 7.07e-16 74.7
MsaG016569 AT5G66300.1 27.778 162 95 6 7 158 12 161 8.08e-16 74.7
MsaG016569 AT1G32870.2 36.220 127 74 2 7 127 10 135 1.26e-15 75.1
MsaG016569 AT1G32870.1 36.220 127 74 2 7 127 10 135 1.30e-15 75.1
MsaG016569 AT5G04395.1 33.103 145 76 4 7 130 28 172 1.31e-15 72.0
MsaG016569 AT1G32870.3 36.220 127 74 2 7 127 46 171 1.76e-15 74.7
MsaG016569 AT3G49530.2 28.571 161 96 5 7 158 13 163 2.05e-15 74.3
MsaG016569 AT3G49530.1 28.571 161 96 5 7 158 13 163 3.25e-15 73.9
MsaG016569 AT3G10480.1 32.571 175 97 7 7 172 27 189 4.20e-15 73.6
MsaG016569 AT1G34190.1 32.283 127 77 2 9 127 18 143 7.73e-15 72.8
MsaG016569 AT1G02230.1 32.090 134 76 4 8 129 4 134 1.05e-14 72.0
MsaG016569 AT3G10490.1 32.000 175 98 7 7 172 27 189 1.12e-14 70.5
MsaG016569 AT5G14000.1 32.934 167 79 7 5 158 13 159 1.28e-14 70.1
MsaG016569 AT5G64060.1 28.235 170 103 4 7 168 6 164 5.50e-14 70.1
MsaG016569 AT3G10490.2 32.000 175 98 7 7 172 27 189 6.94e-14 70.1
MsaG016569 AT2G17040.1 32.143 140 83 4 1 129 1 139 2.63e-13 67.4
MsaG016569 AT5G14000.2 33.099 142 80 5 5 137 13 148 2.68e-13 65.5
MsaG016569 AT1G34180.3 31.496 127 78 2 9 127 18 143 6.28e-13 67.0
MsaG016569 AT1G34180.1 31.496 127 78 2 9 127 18 143 6.62e-13 67.0
MsaG016569 AT3G44350.2 28.395 162 91 6 10 157 8 158 7.86e-13 65.5
MsaG016569 AT5G09330.5 27.879 165 99 5 5 160 4 157 3.57e-12 65.1
MsaG016569 AT5G09330.2 27.879 165 99 5 5 160 4 157 3.57e-12 65.1
MsaG016569 AT5G09330.4 27.879 165 99 5 5 160 4 157 3.57e-12 65.1
MsaG016569 AT5G09330.3 27.879 165 99 5 5 160 4 157 3.57e-12 65.1
MsaG016569 AT5G09330.1 27.879 165 99 5 5 160 4 157 3.57e-12 65.1
MsaG016569 AT3G44350.1 29.412 136 79 4 10 131 8 140 5.26e-12 62.0
MsaG016569 AT5G61430.2 36.111 108 50 6 60 158 5 102 2.51e-11 61.6

Find 46 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCTTCATTCCCACTTTC+TGG 0.138453 3:+99393121 None:intergenic
AATAAATCCAGTACCGAAAA+TGG 0.244913 3:-99393192 MsaT016569.1:CDS
ATTCGCCAATGTGGAATAAT+AGG 0.293651 3:+99393097 None:intergenic
CTTGTTCCTTGGGAATTAAA+TGG 0.298684 3:-99393636 MsaT016569.1:intron
CCAGTACCGAAAATGGATAT+TGG 0.313420 3:-99393185 MsaT016569.1:CDS
ATCTGTCTCTACTTGTTCCT+TGG 0.327451 3:-99393647 MsaT016569.1:CDS
AATGGATATTGGGAGGAAAT+TGG 0.352799 3:-99393174 MsaT016569.1:CDS
TCTGTCTCTACTTGTTCCTT+GGG 0.353376 3:-99393646 MsaT016569.1:CDS
TGGTCAGTGCCTGAAAATAT+TGG 0.355149 3:+99393141 None:intergenic
GAATATCATATTTGCTCCTC+TGG 0.364606 3:-99393039 MsaT016569.1:CDS
TCTAGATCAGGGATGAAGTT+TGG 0.373168 3:+99393669 None:intergenic
TGGCATGACAGGGTGAAAAT+TGG 0.385812 3:-99392847 MsaT016569.1:intron
GATATTGTCAAGCTTCCTCC+TGG 0.398901 3:-99393768 MsaT016569.1:CDS
CAGTACCGAAAATGGATATT+GGG 0.414415 3:-99393184 MsaT016569.1:CDS
CAAAGTACTGTTTCATATCA+TGG 0.427460 3:-99393009 MsaT016569.1:CDS
CACAAAGTTCTGAAACAAGT+TGG 0.433370 3:-99393071 MsaT016569.1:CDS
AGTAGAGACAGATCTAGATC+AGG 0.454456 3:+99393657 None:intergenic
AGGATAAGTTCTTCATCTGT+TGG 0.457202 3:+99393732 None:intergenic
ACAGTGGAACTGATCTTTCA+TGG 0.461699 3:-99392736 MsaT016569.1:CDS
AGGGTGAAAATTGGAGCGAA+TGG 0.500867 3:-99392838 MsaT016569.1:CDS
GGGTGAAAATTGGAGCGAAT+GGG 0.512033 3:-99392837 MsaT016569.1:CDS
ACAAAGTTCTGAAACAAGTT+GGG 0.512054 3:-99393070 MsaT016569.1:CDS
TTCATATCATGGTGATAAAG+TGG 0.528044 3:-99392998 MsaT016569.1:intron
TTCAGGCACTGACCAGAAAG+TGG 0.528880 3:-99393133 MsaT016569.1:CDS
ATATTGTCAAGCTTCCTCCT+GGG 0.550955 3:-99393767 MsaT016569.1:CDS
GGAGTACCATTTAATTCCCA+AGG 0.563304 3:+99393630 None:intergenic
CCTTAGAATAGAGAAAATGA+AGG 0.569373 3:+99393712 None:intergenic
CTATTTCTTCGCCAAAGTGA+AGG 0.570044 3:-99393217 MsaT016569.1:CDS
TAGTGATTTACACCTTCTTG+AGG 0.571144 3:+99392776 None:intergenic
GTAGAGACAGATCTAGATCA+GGG 0.575051 3:+99393658 None:intergenic
AAGAACATGTATCCTCAAGA+AGG 0.582925 3:-99392788 MsaT016569.1:CDS
TCAGGCACTGACCAGAAAGT+GGG 0.598459 3:-99393132 MsaT016569.1:CDS
TACATATAACTACTGAGGCA+TGG 0.622199 3:+99392661 None:intergenic
TACCGAAAATGGATATTGGG+AGG 0.624892 3:-99393181 MsaT016569.1:CDS
TTTGGAGTTGGATGATGACA+TGG 0.636188 3:-99392693 MsaT016569.1:CDS
TTTGTGCTAATTCGCCAATG+TGG 0.638216 3:+99393088 None:intergenic
ACAGATAAGGCATTGTGCGG+TGG 0.648627 3:-99393252 MsaT016569.1:intron
ATGCATACATATAACTACTG+AGG 0.671624 3:+99392656 None:intergenic
GAGTTGGATGATGACATGGA+GGG 0.672071 3:-99392689 MsaT016569.1:CDS
AAGTACCTATTATTCCACAT+TGG 0.679443 3:-99393102 MsaT016569.1:CDS
GTTGGAGAGAAGACAAACCC+AGG 0.681756 3:+99393750 None:intergenic
TGTACAGATAAGGCATTGTG+CGG 0.708392 3:-99393255 MsaT016569.1:intron
GGAGAGAAGACAAACCCAGG+AGG 0.709023 3:+99393753 None:intergenic
AGTACTTTGATCAAATCCAG+AGG 0.713491 3:+99393023 None:intergenic
GGAGTTGGATGATGACATGG+AGG 0.713793 3:-99392690 MsaT016569.1:CDS
AACAGCGATGATGATGACAG+TGG 0.723595 3:-99392752 MsaT016569.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 99392670 99393796 99392670 ID=MsaG016569
Chr3 mRNA 99392670 99393796 99392670 ID=MsaT016569.1;Parent=MsaG016569
Chr3 exon 99392670 99392858 99392670 ID=MsaT016569.1.exon3;Parent=MsaT016569.1
Chr3 CDS 99392670 99392858 99392670 ID=cds.MsaT016569.1;Parent=MsaT016569.1
Chr3 exon 99392999 99393270 99392999 ID=MsaT016569.1.exon2;Parent=MsaT016569.1
Chr3 CDS 99392999 99393270 99392999 ID=cds.MsaT016569.1;Parent=MsaT016569.1
Chr3 exon 99393637 99393796 99393637 ID=MsaT016569.1.exon1;Parent=MsaT016569.1
Chr3 CDS 99393637 99393796 99393637 ID=cds.MsaT016569.1;Parent=MsaT016569.1
Gene Sequence

>MsaT016569.1

ATGGGAGATATTGTCAAGCTTCCTCCTGGGTTTGTCTTCTCTCCAACAGATGAAGAACTTATCCTTCATTTTCTCTATTCTAAGGCTTCTCTTATTCAATCTCATCCAAACTTCATCCCTGATCTAGATCTGTCTCTACTTGTTCCTTGGGAATTAAATGATAAGGCATTGTGCGGTGGAAATGATGAATACTATTTCTTCGCCAAAGTGAAGGAAAATAAATCCAGTACCGAAAATGGATATTGGGAGGAAATTGGTGTTACAGTACCAATATTTTCAGGCACTGACCAGAAAGTGGGAATGAAGAAGTACCTATTATTCCACATTGGCGAATTAGCACAAAGTTCTGAAACAAGTTGGGTAATGCAAGAATATCATATTTGCTCCTCTGGATTTGATCAAAGTACTGTTTCATATCATGGTGATAAAGTGGGTGAAAATTGGAGCGAATGGGTTTTATGCAGAGTCTATGAGATTGATAAGAACATGTATCCTCAAGAAGGTGTAAATCACTACAACAGCGATGATGATGACAGTGGAACTGATCTTTCATGGCAAGACCAAGTTTTTCTGTCTTTGGAGTTGGATGATGACATGGAGGGAATAACCATGCCTCAGTAG
Protein sequence

>MsaT016569.1

MGDIVKLPPGFVFSPTDEELILHFLYSKASLIQSHPNFIPDLDLSLLVPWELNDKALCGGNDEYYFFAKVKENKSSTENGYWEEIGVTVPIFSGTDQKVGMKKYLLFHIGELAQSSETSWVMQEYHICSSGFDQSTVSYHGDKVGENWSEWVLCRVYEIDKNMYPQEGVNHYNSDDDDSGTDLSWQDQVFLSLELDDDMEGITMPQ*